##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938733_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 145278 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.441463951871583 33.0 31.0 34.0 30.0 34.0 2 31.494885667478904 33.0 31.0 34.0 28.0 34.0 3 31.492779360949353 33.0 31.0 34.0 28.0 34.0 4 35.021820234309395 37.0 35.0 37.0 32.0 37.0 5 35.05402056746376 37.0 35.0 37.0 33.0 37.0 6 35.17239361775355 37.0 35.0 37.0 33.0 37.0 7 35.09622241495615 37.0 35.0 37.0 32.0 37.0 8 35.069590715731216 37.0 35.0 37.0 32.0 37.0 9 36.60827516898636 39.0 37.0 39.0 32.0 39.0 10-11 36.49624168834923 39.0 37.0 39.0 32.0 39.0 12-13 36.402955024160576 39.0 37.0 39.0 32.0 39.0 14-15 37.49011206101406 40.0 37.0 41.0 32.0 41.0 16-17 37.42226971736946 40.0 37.0 41.0 32.0 41.0 18-19 37.44477828714602 40.0 37.0 41.0 32.0 41.0 20-21 37.35512947590138 40.0 37.0 41.0 32.0 41.0 22-23 37.27030589628161 40.0 37.0 41.0 32.0 41.0 24-25 37.261722353005965 40.0 37.0 41.0 31.5 41.0 26-27 37.14838103498121 40.0 37.0 41.0 31.0 41.0 28-29 37.00823937554206 40.0 36.5 41.0 31.0 41.0 30-31 36.777860378033836 39.5 36.0 41.0 30.5 41.0 32-33 36.525588870992166 39.0 35.0 41.0 30.0 41.0 34-35 36.168690372940155 39.0 35.0 41.0 30.0 41.0 36-37 35.90703685348092 39.0 35.0 41.0 29.0 41.0 38-39 35.70152053304699 39.0 35.0 41.0 28.0 41.0 40-41 35.4914887319484 39.0 35.0 41.0 27.0 41.0 42-43 35.21982681479646 38.0 35.0 40.0 25.5 41.0 44-45 35.06217734274976 39.0 35.0 41.0 24.5 41.0 46-47 34.76156403584851 39.0 35.0 41.0 23.5 41.0 48-49 34.47432508707443 39.0 34.5 41.0 21.5 41.0 50-51 33.32817425900687 38.0 32.5 40.0 17.5 40.5 52-53 33.43390947011936 38.0 33.0 39.5 18.5 40.5 54-55 33.95951210782087 38.0 34.0 40.0 18.5 41.0 56-57 33.88218794311595 38.0 34.0 40.0 17.0 41.0 58-59 33.675683861286636 38.0 34.0 40.0 14.0 41.0 60-61 33.40091066782307 38.0 33.5 40.0 12.0 41.0 62-63 33.04963587053786 37.0 33.0 40.0 8.0 41.0 64-65 32.67280661903385 37.0 33.0 40.0 7.0 41.0 66-67 32.277657319071025 36.0 32.0 39.0 4.0 41.0 68-69 31.902173763405333 36.0 32.0 39.0 2.0 41.0 70-71 31.504966340395654 35.0 31.0 38.5 2.0 40.0 72-73 31.072375032695934 35.0 31.0 37.5 2.0 40.0 74-75 30.615926017703988 35.0 31.0 37.0 2.0 39.0 76-77 30.193198557248863 35.0 30.0 36.5 2.0 39.0 78-79 29.77042291331103 35.0 30.0 36.0 2.0 39.0 80-81 29.336272525778163 34.0 29.0 36.0 2.0 37.5 82-83 28.877369594845746 34.0 29.0 35.0 2.0 37.0 84-85 28.44201806192266 34.0 29.0 35.0 2.0 36.5 86-87 28.092388386403996 34.0 29.0 35.0 2.0 36.0 88-89 27.711367171904897 34.0 27.0 35.0 2.0 36.0 90-91 27.28835749390823 34.0 26.0 35.0 2.0 35.5 92-93 26.85239334241936 34.0 25.5 35.0 2.0 35.0 94-95 26.3707925494569 33.5 25.0 35.0 2.0 35.0 96-97 25.827795674499924 33.0 22.0 35.0 2.0 35.0 98-99 25.219940390148544 33.0 17.0 35.0 2.0 35.0 100-101 23.10763157532455 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.321985005328127 1101 2 -0.3085379849591483 1101 3 0.41702183123387115 1101 4 0.4492932490804833 1101 5 0.04153210854110512 1101 6 0.2582882929164185 1101 7 0.5518201124474587 1101 8 0.20545087086801317 1101 9 0.6457291859877685 1101 10-11 0.2942020785249184 1101 12-13 0.5699362910253214 1101 14-15 0.2802336095677376 1101 16-17 0.48156390561410234 1101 18-19 -0.03277918641615685 1101 20-21 0.08599438768074208 1101 22-23 0.4024055724438469 1101 24-25 0.2800841616373333 1101 26-27 0.31161995703135403 1101 28-29 -0.04702515834833321 1101 30-31 0.3663347789518667 1101 32-33 0.15357628263232215 1101 34-35 0.10446364673973108 1101 36-37 0.38612570118458933 1101 38-39 -0.022356796411564517 1101 40-41 0.25689329111011716 1101 42-43 0.2892315590283161 1101 44-45 0.2505388359781904 1101 46-47 -0.31358996466899214 1101 48-49 -0.7495084314546929 1101 50-51 -0.8121726117781094 1101 52-53 -0.7582558948782392 1101 54-55 -0.4604761680369478 1101 56-57 -0.1675731557852984 1101 58-59 -0.3738716358520122 1101 60-61 -0.5421430585817006 1101 62-63 -0.5043773586187115 1101 64-65 -0.8859331070170384 1101 66-67 -0.3694214841448087 1101 68-69 0.18873190263783357 1101 70-71 -0.43751162841653013 1101 72-73 -0.3008366699237506 1101 74-75 -0.9464821579552769 1101 76-77 -0.2155544010120849 1101 78-79 -0.5075685027942285 1101 80-81 -0.3092651772010946 1101 82-83 -0.4920216471927219 1101 84-85 -0.5391946951314388 1101 86-87 -0.7763742888654406 1101 88-89 -1.5097523674514832 1101 90-91 -1.1587937931867458 1101 92-93 -1.0625704028767018 1101 94-95 -1.307642998377876 1101 96-97 -1.2774751551396832 1101 98-99 -0.7303618945594259 1101 100-101 -1.7072729154994164 1102 1 -0.12057004154346629 1102 2 0.24836194963862823 1102 3 0.39091544361669506 1102 4 0.07121389438242431 1102 5 0.5303047268101295 1102 6 0.04643959006052256 1102 7 -0.04066953220046088 1102 8 0.08794487566417075 1102 9 -0.1472727760789425 1102 10-11 0.30788204364372973 1102 12-13 0.40918307541598864 1102 14-15 0.25246491543214233 1102 16-17 -0.2597975506754082 1102 18-19 -5.958421824487914E-4 1102 20-21 -0.07606687240209453 1102 22-23 0.06247751488989195 1102 24-25 0.45326379974502373 1102 26-27 0.12909325112335068 1102 28-29 -0.08566697217000296 1102 30-31 0.31493953151345266 1102 32-33 0.2543774598723516 1102 34-35 0.5957978521027059 1102 36-37 0.5561714827411706 1102 38-39 0.5661269620362219 1102 40-41 0.7150903698107882 1102 42-43 0.3681229913370174 1102 44-45 0.7770539874933462 1102 46-47 1.2210187120260159 1102 48-49 1.0977228744097047 1102 50-51 0.9895223958521555 1102 52-53 0.4551733617164899 1102 54-55 0.38250399328853746 1102 56-57 0.6851628372384653 1102 58-59 0.7084806641261849 1102 60-61 0.6700599063191106 1102 62-63 0.41866602485632853 1102 64-65 0.15611507262106272 1102 66-67 -0.41375874161155934 1102 68-69 -0.47910655386969125 1102 70-71 0.026244639296571393 1102 72-73 0.25938787771087135 1102 74-75 0.4941380731325786 1102 76-77 0.41996445662907433 1102 78-79 -0.10918720783019964 1102 80-81 0.4068891720467853 1102 82-83 0.02898881716306434 1102 84-85 -0.09770243439457715 1102 86-87 0.173484006316599 1102 88-89 0.20937233278832323 1102 90-91 0.7994850382935255 1102 92-93 0.7582002533256116 1102 94-95 0.4547016713408958 1102 96-97 -0.32598180436575674 1102 98-99 0.34288859595725185 1102 100-101 -0.280169143971186 1103 1 0.10127039655381509 1103 2 0.47165197433529116 1103 3 0.03721631336865272 1103 4 0.47156823777338275 1103 5 0.1138975540408822 1103 6 0.1091476325817311 1103 7 0.271743224161618 1103 8 0.2452518658928895 1103 9 0.25292050213430883 1103 10-11 0.4938854797008503 1103 12-13 0.4796490906634929 1103 14-15 0.6744556810286184 1103 16-17 0.7586444121722238 1103 18-19 0.7432836810646251 1103 20-21 0.2501169566540611 1103 22-23 0.6524055724438469 1103 24-25 0.44601586674814797 1103 26-27 0.10933592265777037 1103 28-29 0.6499445386213623 1103 30-31 0.706113159775029 1103 32-33 0.10456000040708346 1103 34-35 0.6882492641074336 1103 36-37 0.4509153891086086 1103 38-39 0.1707119507616639 1103 40-41 0.560602020191979 1103 42-43 0.5332953310771629 1103 44-45 -0.01772213190963612 1103 46-47 0.520761007741676 1103 48-49 0.6950867743345341 1103 50-51 1.1882231367519722 1103 52-53 1.0651271896988774 1103 54-55 0.29042840003180004 1103 56-57 0.3820650169872053 1103 58-59 0.9021346961244703 1103 60-61 0.7179767966874095 1103 62-63 0.45469093627047386 1103 64-65 0.458085436628366 1103 66-67 0.5284188595910564 1103 68-69 -0.27039292775565826 1103 70-71 -0.20944740408364737 1103 72-73 0.15891005101699918 1103 74-75 0.7016973988063704 1103 76-77 0.1449725098879604 1103 78-79 0.7144464225789058 1103 80-81 0.8566099924054207 1103 82-83 0.8622863582346874 1103 84-85 0.18067188107842114 1103 86-87 0.6874429883846744 1103 88-89 0.6724615628967747 1103 90-91 0.34216731038629433 1103 92-93 0.7121356125009584 1103 94-95 0.8580625647157483 1103 96-97 0.8515388656653826 1103 98-99 0.8211984853591652 1103 100-101 0.9790115711582033 1104 1 0.5126776460207658 1104 2 0.5356178591966945 1104 3 0.3200521342641771 1104 4 0.38457463436186856 1104 5 0.5757312427402397 1104 6 0.5658640504921735 1104 7 0.1295257401530847 1104 8 0.329260394677533 1104 9 0.256118796377379 1104 10-11 0.3301328144556521 1104 12-13 0.3778367239257534 1104 14-15 0.4145409688751016 1104 16-17 0.39952927357946777 1104 18-19 0.38843293479596497 1104 20-21 0.0429207946071557 1104 22-23 0.36311985815813586 1104 24-25 0.2796512612044282 1104 26-27 -0.055696060284667226 1104 28-29 0.049944538621360834 1104 30-31 0.26005772267480864 1104 32-33 0.36948537354141564 1104 34-35 0.5538142960903798 1104 36-37 0.6770347920936857 1104 38-39 0.18467783562307005 1104 40-41 0.5768067110235364 1104 42-43 -0.08706714227038503 1104 44-45 0.8270539874933434 1104 46-47 0.7164966154175829 1104 48-49 0.8097989278526612 1104 50-51 1.1036282540227589 1104 52-53 1.0155536289312863 1104 54-55 0.4537013211405423 1104 56-57 0.026041562829419718 1104 58-59 0.1828383208579467 1104 60-61 -0.12482704126568223 1104 62-63 0.024626970385618563 1104 64-65 0.17045217436823634 1104 66-67 0.025167260443936357 1104 68-69 0.46665398055990437 1104 70-71 0.3820771161722192 1104 72-73 0.4986222045351276 1104 74-75 -0.572023283496403 1104 76-77 -0.48167994213762455 1104 78-79 -0.7789970742228007 1104 80-81 -1.0884859564218736 1104 82-83 -1.22838528355636 1104 84-85 -0.7545626604994062 1104 86-87 -0.5970452845364349 1104 88-89 -1.3549904626895817 1104 90-91 -2.0015427109356665 1104 92-93 -2.0144102297165247 1104 94-95 -2.105370271105148 1104 96-97 -2.165678618343147 1104 98-99 -2.4401021542996872 1104 100-101 -3.283355166581668 1105 1 0.016120605938546362 1105 2 -0.11854452518151604 1105 3 0.09595141483971403 1105 4 0.006465692943571355 1105 5 -0.15314851059994083 1105 6 -0.6585963811624964 1105 7 -0.5370724098983004 1105 8 -0.09910548404805297 1105 9 -0.24079795593505793 1105 10-11 -0.05183018657209715 1105 12-13 -0.23110469436817738 1105 14-15 0.18771671399328937 1105 16-17 -0.06195582405670308 1105 18-19 -0.38548792851338476 1105 20-21 -0.19477190837331904 1105 22-23 0.31067895373880816 1105 24-25 -0.3057290060103668 1105 26-27 -0.5723455977975149 1105 28-29 -0.4058108570620931 1105 30-31 -0.19944895769518212 1105 32-33 0.002578898721274925 1105 34-35 -0.21355466588290284 1105 36-37 0.5058117705109524 1105 38-39 0.1992204872160812 1105 40-41 0.01724864319208308 1105 42-43 -0.1750424762888798 1105 44-45 0.036046793248743825 1105 46-47 0.15267338828500243 1105 48-49 0.3171473348413585 1105 50-51 0.2665008131183413 1105 52-53 0.4443819948100156 1105 54-55 0.09833133141803785 1105 56-57 0.3506304631377475 1105 58-59 0.6653151965002877 1105 60-61 1.1592685394126292 1105 62-63 0.5517595500361878 1105 64-65 0.6812949287361718 1105 66-67 0.7912772296114596 1105 68-69 0.49571359001519966 1105 70-71 -0.002532339120691063 1105 72-73 0.2485965108043935 1105 74-75 0.5480949076649573 1105 76-77 0.1969428738952601 1105 78-79 0.736136533176996 1105 80-81 0.6335078770827529 1105 82-83 0.3311470905443592 1105 84-85 0.42747742172053194 1105 86-87 0.07995882646048358 1105 88-89 7.392392631437872E-4 1105 90-91 0.6016433116748168 1105 92-93 0.36971104469251515 1105 94-95 0.9511045490387353 1105 96-97 1.3286944546270512 1105 98-99 1.5587159340867451 1105 100-101 1.7629963236547148 1106 1 0.08340199209722599 1106 2 0.217710414528689 1106 3 0.19540424694023173 1106 4 0.18819636474416512 1106 5 0.29142540773018055 1106 6 0.4489626420414723 1106 7 0.4627249353241112 1106 8 0.42845556570369325 1106 9 -0.11611460402503582 1106 10-11 -0.3023621553632623 1106 12-13 -0.2117004994142846 1106 14-15 0.5135349326577909 1106 16-17 0.17976066190944806 1106 18-19 0.19230617423259133 1106 20-21 0.3075586215689228 1106 22-23 0.07846191047202211 1106 24-25 0.14046615054045475 1106 26-27 0.37478683709964145 1106 28-29 0.20628256679037804 1106 30-31 0.43078206875127023 1106 32-33 0.2532881904428237 1106 34-35 0.5314802920662345 1106 36-37 0.6601333836429788 1106 38-39 0.26184081349027366 1106 40-41 0.07016687198932914 1106 42-43 0.4865747088362582 1106 44-45 0.9601525790426422 1106 46-47 0.9717280037475646 1106 48-49 1.2355534550156406 1106 50-51 1.1580045115680306 1106 52-53 1.5774248562954725 1106 54-55 1.420552427780379 1106 56-57 1.862409772084952 1106 58-59 1.7382709164313823 1106 60-61 1.5020844275471958 1106 62-63 1.5067195257176067 1106 64-65 1.5400698806056639 1106 66-67 1.6027829546089265 1106 68-69 1.7970865761333457 1106 70-71 1.2794111201963574 1106 72-73 1.2486725063459936 1106 74-75 1.3054918070468595 1106 76-77 1.2179176433754542 1106 78-79 1.3674314972057715 1106 80-81 1.2233852709423125 1106 82-83 1.0881640122182894 1106 84-85 0.927781403886911 1106 86-87 0.8979748361879132 1106 88-89 0.9943556137691729 1106 90-91 0.62391000536212 1106 92-93 0.9976622048911175 1106 94-95 1.2418128274863989 1106 96-97 1.0178726895039354 1106 98-99 1.0586906022395475 1106 100-101 1.330125718730205 1107 1 0.21016255547750617 1107 2 0.25996393565544906 1107 3 0.24470002158811965 1107 4 0.32904143516669393 1107 5 0.2632563936456762 1107 6 0.06868095190062462 1107 7 0.5049784564508712 1107 8 0.44254007274594187 1107 9 0.6303642692143967 1107 10-11 0.19763784463673773 1107 12-13 0.7284403456561392 1107 14-15 0.5663518340662392 1107 16-17 0.13398601402212051 1107 18-19 0.6183625122607523 1107 20-21 0.5153051004421627 1107 22-23 0.5573351499086314 1107 24-25 0.8130013618080554 1107 26-27 0.7938009216066817 1107 28-29 0.5619163696072818 1107 30-31 1.2723313645259182 1107 32-33 0.946950162273815 1107 34-35 0.4716211371366512 1107 36-37 -0.07578210931476548 1107 38-39 0.15972813743393743 1107 40-41 0.35185701283440096 1107 42-43 0.8492507651742827 1107 44-45 0.9883215931271536 1107 46-47 0.9400378629024928 1107 48-49 1.0242858493818332 1107 50-51 0.8340608495961988 1107 52-53 0.6196783774222396 1107 54-55 1.0015383432733387 1107 56-57 0.6194520256060798 1107 58-59 0.8720737333327904 1107 60-61 0.6887041458570522 1107 62-63 0.8940434693795822 1107 64-65 1.0435910073662313 1107 66-67 1.0957407010877986 1107 68-69 0.7231429141615209 1107 70-71 0.9026505568160843 1107 72-73 0.9000809570502177 1107 74-75 0.4745058915538998 1107 76-77 0.41862186872756624 1107 78-79 0.6174314972057715 1107 80-81 0.040286679393016556 1107 82-83 0.45788232207744173 1107 84-85 0.4383447841686028 1107 86-87 0.7747353995681934 1107 88-89 0.3852006841917053 1107 90-91 0.7295438081790238 1107 92-93 1.0610424865812575 1107 94-95 0.9108269119934427 1107 96-97 0.5847740979546394 1107 98-99 -0.20187277804214077 1107 100-101 0.189280648307669 1108 1 -0.013191079847960197 1108 2 -0.04256746898863284 1108 3 0.40924132345336517 1108 4 -0.04476899498175868 1108 5 0.03866560567460198 1108 6 -0.21386567923754995 1108 7 0.009641570268918542 1108 8 0.27752294294008095 1108 9 0.11133115158973084 1108 10-11 -0.05673977241693251 1108 12-13 0.23691008299911687 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-1.0299870664952095 2316 56-57 -1.0625758139458483 2316 58-59 -1.4165351127260166 2316 60-61 -0.8304870245572431 2316 62-63 -1.790661250165762 2316 64-65 -1.763591267569936 2316 66-67 -1.2828319041341771 2316 68-69 -1.294853955948028 2316 70-71 -1.5811852063006313 2316 72-73 -1.5460519809285351 2316 74-75 -1.6544167672056744 2316 76-77 -1.5617133590131473 2316 78-79 -1.8913404326187901 2316 80-81 -1.8725293983600508 2316 82-83 -1.570636745544661 2316 84-85 -2.0230463697725867 2316 86-87 -1.8789792862072794 2316 88-89 -2.3832903791473896 2316 90-91 -2.6309495613951484 2316 92-93 -1.9891387176029092 2316 94-95 -2.42320652841509 2316 96-97 -3.140845702720757 2316 98-99 -3.5974538669145595 2316 100-101 -2.97182634452653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1375.0 3 272.0 4 218.0 5 228.0 6 250.0 7 283.0 8 318.0 9 338.0 10 451.0 11 565.0 12 701.0 13 975.0 14 1043.0 15 1111.0 16 1464.0 17 1858.0 18 1534.0 19 996.0 20 916.0 21 1030.0 22 1116.0 23 1328.0 24 1428.0 25 1513.0 26 1788.0 27 2049.0 28 2450.0 29 2953.0 30 3482.0 31 4168.0 32 5190.0 33 6489.0 34 8756.0 35 12994.0 36 17412.0 37 25867.0 38 25685.0 39 4682.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.567937836552055 20.434785606431465 14.350820825030855 20.64645573198563 2 14.78209942467358 22.124918179625865 40.67041030764461 22.422572088055947 3 19.41160259060218 28.852142758715722 29.402645721372465 22.33360892930963 4 11.698308238293768 17.61154946077249 39.41150122316783 31.27864107776591 5 12.15855751535328 39.50152671229572 33.76067492400902 14.579240848341984 6 28.03220208252989 38.173516610655064 18.626384221255027 15.167897085560025 7 26.727961432506888 34.833333333333336 22.00275482093664 16.43595041322314 8 25.603482117645438 33.39485809130917 21.07521401662546 19.926445774419935 9 27.509211749715902 17.74785633114088 21.828575364165435 32.914356554977786 10-11 23.83521925082129 27.800811300353306 27.371744984469593 20.992224464355814 12-13 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27.480155642023345 31.53065283182015 20.341720709035886 38-39 20.33775816819444 27.866431997123108 31.998506229231573 19.79730360545088 40-41 19.97573126000394 28.033506072370628 32.097655750343115 19.893106917282317 42-43 19.833324688348288 28.28224147172225 31.05244047927797 20.831993360651484 44-45 20.07193373796061 28.272379865470075 30.907644688834708 20.748041707734604 46-47 20.431799475726407 28.26650804739212 30.350460987266654 20.95123148961482 48-49 20.3895483978084 28.692096961647017 30.136213957606948 20.782140682937627 50-51 20.382372675017812 28.668266803162464 30.243963173803873 20.705397348015854 52-53 20.301875306959598 29.18104909278308 29.834397459930965 20.682678140326363 54-55 19.930692692644225 28.534274953570325 30.28840001798393 21.246632335801515 56-57 20.57178027814469 29.109411414858815 29.050974751211957 21.267833555784538 58-59 20.623364411672178 28.418876547435083 29.79856120082 21.159197840072736 60-61 20.64127286927793 28.669970486795087 29.95521181081137 20.733544833115612 62-63 20.70655199720588 28.550136766501026 30.28055287555459 20.462758360738505 64-65 20.38404062487028 28.478573701761427 30.25764137759267 20.879744295775623 66-67 20.216206866730086 28.882469947245525 29.570526679927355 21.330796506097034 68-69 20.161842751672744 28.90716356566083 29.94921223611467 20.981781446551757 70-71 20.681817395546116 28.35970122919485 29.862550207402844 21.09593116785619 72-73 20.357065023747833 28.391546947374245 29.672857592561204 21.57853043631672 74-75 20.19564029304536 29.08834944068184 29.545627494794225 21.17038277147858 76-77 20.359399479161265 28.249449245890528 29.958948500600037 21.432202774348173 78-79 20.06797546521357 28.33982200524165 30.097987013436047 21.494215516108735 80-81 20.38311261712942 29.027350368244527 29.28425711420767 21.30527990041838 82-83 20.69853894700441 28.53082043745137 29.19996541886401 21.570675196680213 84-85 20.142334971315144 28.230115119805514 29.507879257340658 22.119670651538677 86-87 20.500982182381584 28.383341633466134 29.61556551571492 21.50011066843736 88-89 20.766594148511185 27.87913794475016 29.463584702704388 21.89068320403427 90-91 20.335999003832477 28.02897187210316 29.845939925564146 21.789089198500214 92-93 20.87021490759285 28.439541890204463 29.359635559890833 21.330607642311854 94-95 20.591307837059997 27.870060327651096 29.43913272083241 22.099499114456496 96-97 20.521361933522204 27.708076059621778 29.425886145789015 22.344675861067007 98-99 20.805733596676617 27.883833789341516 28.879326745139277 22.431105868842593 100-101 21.2241735894474 27.17834143777106 29.49415857952148 22.10332639326006 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 559.0 1 341.0 2 191.5 3 239.5 4 174.5 5 168.5 6 314.5 7 589.0 8 827.0 9 859.5 10 676.0 11 493.5 12 445.0 13 442.0 14 432.0 15 405.0 16 394.5 17 410.5 18 414.0 19 408.5 20 418.0 21 455.0 22 508.5 23 595.5 24 665.0 25 717.5 26 865.5 27 1002.0 28 1118.5 29 1373.0 30 1746.5 31 2092.5 32 2571.0 33 3165.5 34 3670.5 35 4210.0 36 5054.5 37 5630.0 38 5512.5 39 5300.5 40 5017.0 41 4698.5 42 4283.5 43 4262.5 44 4594.0 45 4677.0 46 4526.0 47 4824.5 48 6869.5 49 6798.5 50 4810.0 51 4035.0 52 3407.5 53 3152.0 54 3036.5 55 2858.5 56 2622.0 57 2390.0 58 2232.0 59 2068.0 60 1848.0 61 1591.5 62 1294.0 63 977.0 64 732.5 65 553.5 66 427.0 67 340.5 68 253.5 69 189.5 70 150.5 71 114.0 72 84.0 73 76.0 74 76.0 75 70.0 76 47.5 77 28.0 78 23.5 79 17.0 80 10.5 81 6.0 82 5.0 83 6.0 84 6.5 85 4.0 86 1.0 87 1.5 88 1.0 89 0.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16588884758876088 2 0.09843197180577927 3 0.09499029446991286 4 0.11219868114924489 5 0.1342254160987899 6 0.04818348270212971 7 0.05369016643951596 8 0.05437850190668925 9 0.057131843775382364 10-11 0.05437850190668925 12-13 0.06126185657842206 14-15 0.05919685017690222 16-17 0.054034334173102605 18-19 0.061950192045595334 20-21 0.0633268629799419 22-23 0.0777819077905808 24-25 0.09223695260121972 26-27 0.0953344622034995 28-29 0.08776277206459339 30-31 0.07743774005699418 32-33 0.06435936618070183 34-35 0.31284846983025644 36-37 0.49250402676248295 38-39 0.4663472790098983 40-41 0.44569721499469983 42-43 0.4718539627472845 44-45 0.4821789947548837 46-47 0.4797698206197773 48-49 0.49973154916780244 50-51 0.48665317529151003 52-53 0.49215985902889636 54-55 0.4849323366235768 56-57 0.4660031112763116 58-59 0.4429438731260067 60-61 0.4109362739024491 62-63 0.4739189691488043 64-65 0.5066149038395352 66-67 0.509712413441815 68-69 0.5196932777158275 70-71 0.5179724390478944 72-73 0.5083357425074685 74-75 0.5004198846349758 76-77 0.4842440011564036 78-79 0.4591197566045788 80-81 0.4632497694076185 82-83 0.47529564008315095 84-85 0.47460730461597767 86-87 0.4832114979556437 88-89 0.4931923622296563 90-91 0.4983548782334558 92-93 0.5007640523685624 94-95 0.5052382329051887 96-97 0.5048940651716021 98-99 0.5000757169013891 100-101 0.48665317529151003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 145278.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.71959966409229 #Duplication Level Percentage of deduplicated Percentage of total 1 85.08894217364636 52.51655446798552 2 8.296436736742319 10.241055080604083 3 2.3453967545865164 4.342708462396233 4 1.1955612557854236 2.951582483239031 5 0.635699548318742 1.9617560814438526 6 0.41933864941727544 1.5528848139429232 7 0.30112083867729883 1.3009540329575022 8 0.2342050967490102 1.156403584851113 9 0.19963196341939443 1.1089084376161567 >10 1.175486533206937 12.762427896859812 >50 0.06914626665923158 2.9715442117870565 >100 0.03234260859867284 3.8312752102864853 >500 0.005576311827357386 2.323132201709825 >1k 0.0011152623654714772 0.9788130343204063 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1422 0.9788130343204063 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 974 0.6704387450267763 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 711 0.48940651716020317 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 595 0.4095596029681025 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 569 0.3916628808215973 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 526 0.3620644557331461 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 403 0.27739919327083246 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 315 0.21682567215958368 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 304 0.20925398202067758 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 299 0.2058123046848112 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 288 0.1982406145459051 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 275 0.18929225347265244 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 250 0.17208386679332038 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 237 0.16313550572006771 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 222 0.1528104737124685 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 213 0.14661545450790897 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 208 0.14317377717204255 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 183 0.12596539049271055 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 175 0.12045870675532427 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 170 0.11701702941945788 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 170 0.11701702941945788 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.10669199741185866 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 153 0.10531532647751207 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 149 0.10256198460881895 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0027533418686931264 0.0 24-25 0.0 0.0 0.0 0.004130012803039689 0.0 26-27 0.0 0.0 0.0 0.004130012803039689 0.0 28-29 0.0 0.0 0.0 0.013078373876292348 0.0 30-31 0.0 0.0 0.0 0.028910089621277828 0.0 32-33 0.0 0.0 0.0 0.06263852751276862 0.0 34-35 0.0 0.0 0.0 0.1139195198171781 0.0 36-37 0.0 0.0 0.0 0.18206473106733298 0.0 38-39 0.0 0.0 0.0 0.28875672847919165 0.0 40-41 0.0 0.0 0.0 0.4143779512383155 0.0 42-43 0.0 0.0 0.0 0.5276091355883203 0.0 44-45 0.0 0.0 0.0 0.6532303583474441 0.0 46-47 0.0 0.0 0.0 0.815333360866752 0.0 48-49 0.0 0.0 0.0 0.9863847244593125 0.0 50-51 0.0 0.0 0.0 1.1749886424647915 0.0 52-53 0.0 0.0 0.0 1.3608392186015776 0.0 54-55 0.0 0.0 0.0 1.5542614848772698 0.0 56-57 0.0 0.0 0.0 1.7962114015886783 0.0 58-59 0.0 0.0 0.0 2.0701689175236444 0.0 60-61 0.0 0.0 0.0 2.3978165998981265 0.0 62-63 0.0 0.0 0.0 2.6889825025124248 0.0 64-65 0.0 0.0 0.0 2.986687592064869 0.0 66-67 0.0 0.0 0.0 3.3188094549759772 0.0 68-69 0.0 0.0 0.0 3.72630405154256 0.0 70-71 0.0 0.0 0.0 4.175442943873126 0.0 72-73 0.0 0.0 0.0 4.627679345805972 0.0 74-75 0.0 0.0 0.0 5.095747463483804 0.0 76-77 0.0 0.0 0.0 5.568978097165434 0.0 78-79 0.0 0.0 0.0 6.124120651440686 0.0 80-81 0.0 0.0 0.0 6.74052506229436 0.0 82-83 0.0 0.0 0.0 7.36346866008618 0.0 84-85 0.0 0.0 0.0 8.001211470422225 0.0 86-87 0.0 0.0 0.0 8.645493467696417 0.0 88-89 0.0 0.0 0.0 9.301133000178968 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCACCG 20 5.355443E-4 47.546696 56-57 CTTACTA 105 1.0459189E-9 40.58585 9 CGCATCC 25 0.0016033163 38.050518 88-89 CACGCAT 25 0.0016033163 38.050518 86-87 TGCACGC 25 0.0016060445 38.037357 84-85 ACTGTAG 55 0.0026124027 34.436474 8 ATATAGG 130 8.409188E-9 32.79217 2 GATTCTA 45 2.368128E-5 31.741716 32-33 CCGGGTT 30 0.003920522 31.708763 60-61 ATAACTC 30 0.0039271694 31.697798 84-85 GATATAG 150 9.622454E-10 31.599426 1 GCCCGAC 30 0.004004259 31.572206 26-27 AGGCTTA 140 1.736953E-8 30.439386 6 CAATACT 95 3.5374043E-5 29.915667 4 CACCGGG 40 4.4506486E-4 29.726969 58-59 GCCGGAG 40 4.459679E-4 29.716686 38-39 GGGACAG 80 4.548903E-4 29.604044 5 ACTGGGC 80 4.558098E-4 29.593845 8 TACACTG 65 0.0059081926 29.148596 5 TATAGGC 150 3.385867E-8 28.419884 3 >>END_MODULE