FastQCFastQC Report
Thu 26 May 2016
SRR938733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145278
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14561.002216440204298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.657360371150484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.5293299742562535No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6560.4515480664656727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6340.4364046861878605No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5830.40129957736202315No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4170.28703588981125844No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.21820234309393025No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.2051239692176379No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2670.18378556973526616No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2660.18309723426809288No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2620.18034389239939977No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.16657718305593414No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2210.15212213824529522No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2190.15074546731094868No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.13629042250030976No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1970.13560208703313648No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1840.1266537259598838No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT1760.12114704222249756No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.11977037128815099No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC1720.11839370035380443No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1620.11151034568207162No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG1590.10944533928055178No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.10738033287903193No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1470.1011853136744724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG707.923154E-847.4493982
CTGGTCG554.973015E-543.1358159
GTAGGAT459.647759E-442.1772423
CATGGGT853.641817E-739.0759774
CATTCCG500.001619630137.959529
ACCCAGG702.0201928E-433.9977571
GTATATA1051.8567807E-631.731241
GTATAGG902.365369E-531.7312371
AGCGTTT300.0039667831.63292930-31
TTAGGCA600.003960306831.6329294
ACTACGT300.0039667831.63292916-17
CAGTCCC1102.7344558E-630.195079
CCATATC1004.959685E-528.4696399
AAGAGAG856.4199447E-427.9114115
ATAACCC856.4199447E-427.9114113
ACCTGGG1205.383785E-627.6788143
ATTTTCG350.00812518827.31610982-83
GAAGCGT350.0084251227.11394128-29
ATATAGG700.00841149627.1139412
GCCCGAA350.0084251227.11394124-25
Produced by FastQC (version 0.11.5)