FastQCFastQC Report
Thu 26 May 2016
SRR938732_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938732_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145241
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14821.0203730351622475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.6561508114100013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.5494316343181334No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6080.4186145785281016No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5990.41241798114857375No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5590.38487754835067234No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4390.3022562499569681No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3590.24717538436116523No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT3100.21343835418373597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.20035664860473282No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2870.19760260532494267No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.18452089974593952No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.1673081292472511No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2430.1673081292472511No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2280.15698046694803808No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT2080.14321025054908737No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1970.1356366315296645No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.13150556660997928No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1740.11980088267087116No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.11566981775118597No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1580.1087847095517106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTGG704.3511463E-640.6222468
AGTAGTG500.001629150637.9140977
CTGTTAG651.3265546E-436.519111
CTACCTC550.002578866234.527161
TCCCCGG550.002600895734.467366
CTATACA600.003976749331.6060314
TATTAAG600.003976749331.6060312
GGCTAGG650.005842449629.215291
CTCCCCG650.00588220729.1747955
TAAGTAG650.00588220729.1747955
AAGTAGT650.00589217929.164696
TAGACGA650.00589217929.164699
AGTATGA650.00589217929.164696
CATGGGT1004.988309E-528.4454274
CATGGGG2350.028.2436854
TACCTCC700.00844629827.0908832
AGTACTC700.00844629827.0908835
TAGTGGG700.00846059227.0814979
CACAGGG950.001237584324.9434877
GTACATG12150.024.6165871
Produced by FastQC (version 0.11.5)