##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938732_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 145241 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41707919939962 33.0 31.0 34.0 30.0 34.0 2 31.448599224736817 33.0 31.0 34.0 28.0 34.0 3 31.458018052753697 33.0 31.0 34.0 28.0 34.0 4 35.001473413154685 37.0 35.0 37.0 32.0 37.0 5 35.041503432226435 37.0 35.0 37.0 33.0 37.0 6 35.10918404582728 37.0 35.0 37.0 32.0 37.0 7 35.03117576992722 37.0 35.0 37.0 32.0 37.0 8 35.00722936360945 37.0 35.0 37.0 32.0 37.0 9 36.555869210484644 39.0 37.0 39.0 32.0 39.0 10-11 36.437703541011146 39.0 37.0 39.0 32.0 39.0 12-13 36.35196328860308 39.0 37.0 39.0 32.0 39.0 14-15 37.432088735274476 40.0 37.0 41.0 32.0 41.0 16-17 37.34441032490825 40.0 37.0 41.0 32.0 41.0 18-19 37.355598625732405 40.0 37.0 41.0 32.0 41.0 20-21 37.27204095262357 40.0 37.0 41.0 32.0 41.0 22-23 37.26019512396637 40.0 37.0 41.0 31.5 41.0 24-25 37.18285470356167 40.0 37.0 41.0 31.0 41.0 26-27 37.03310360022308 40.0 37.0 41.0 31.0 41.0 28-29 36.84712305753885 40.0 36.0 41.0 30.5 41.0 30-31 36.58356800077113 39.0 36.0 41.0 30.0 41.0 32-33 36.33134583209975 39.0 35.0 41.0 30.0 41.0 34-35 36.03521388588622 39.0 35.0 41.0 29.5 41.0 36-37 35.81559614709345 39.0 35.0 41.0 28.5 41.0 38-39 35.612953642566495 39.0 35.0 41.0 27.0 41.0 40-41 35.489692993025386 39.0 35.0 40.5 27.0 41.0 42-43 35.41541988832354 39.0 35.0 41.0 26.5 41.0 44-45 35.180241804999966 39.0 35.0 41.0 24.5 41.0 46-47 34.76965870518655 39.0 35.0 41.0 23.5 41.0 48-49 34.413003215345526 39.0 34.5 41.0 21.0 41.0 50-51 33.271944561108775 38.0 32.5 40.0 17.0 40.5 52-53 33.3688318036918 38.0 33.0 39.5 18.0 40.5 54-55 33.85755399646106 38.0 34.0 40.0 17.5 41.0 56-57 33.78295729167384 38.0 34.0 40.0 14.5 41.0 58-59 33.58478666492244 38.0 34.0 40.0 13.0 41.0 60-61 33.28859619528921 38.0 33.0 40.0 9.5 41.0 62-63 32.89560110437135 37.0 33.0 40.0 7.5 41.0 64-65 32.51261351822144 36.5 32.0 40.0 4.5 41.0 66-67 32.128765293546586 36.0 32.0 39.0 2.0 41.0 68-69 31.730509979964335 35.5 31.5 39.0 2.0 40.5 70-71 31.304648825056283 35.0 31.0 38.5 2.0 40.0 72-73 30.862786678692657 35.0 31.0 37.5 2.0 40.0 74-75 30.412648632273257 35.0 30.0 37.0 2.0 39.0 76-77 29.945074049338686 35.0 30.0 36.5 2.0 39.0 78-79 29.496020407460705 34.5 29.0 36.0 2.0 38.5 80-81 29.035358473158404 34.0 29.0 36.0 2.0 37.5 82-83 28.625553390571532 34.0 29.0 35.0 2.0 37.0 84-85 28.265796159486648 34.0 28.5 35.0 2.0 36.5 86-87 27.857144332523184 34.0 27.5 35.0 2.0 36.0 88-89 27.383132173422105 34.0 26.5 35.0 2.0 36.0 90-91 26.90382192356153 34.0 25.5 35.0 2.0 35.5 92-93 26.455697771290474 33.5 25.0 35.0 2.0 35.0 94-95 25.972383831011904 33.0 23.5 35.0 2.0 35.0 96-97 25.38244710515626 33.0 19.0 35.0 2.0 35.0 98-99 24.6758663187392 32.5 4.5 35.0 2.0 35.0 100-101 22.305857161545294 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8119763151897494 1101 2 1.2466351417329946 1101 3 0.9423098611587015 1101 4 0.9103382368806976 1101 5 0.9295735872623894 1101 6 1.0071099976375137 1101 7 1.0114885267347518 1101 8 0.9949738960344234 1101 9 1.1240996477366423 1101 10-11 0.8174246134783658 1101 12-13 0.7074801940513993 1101 14-15 0.741603434575218 1101 16-17 1.3876901414005687 1101 18-19 1.6357649642823162 1101 20-21 1.3110922095294484 1101 22-23 1.1186774085500772 1101 24-25 1.0384847250869527 1101 26-27 1.0214560484059874 1101 28-29 1.5222649107001658 1101 30-31 1.0341286694151464 1101 32-33 0.45719193442266004 1101 34-35 0.6543130208880683 1101 36-37 1.001106849876983 1101 38-39 0.8119258745056399 1101 40-41 0.39010504206429175 1101 42-43 0.7566359314831104 1101 44-45 0.8982680094014697 1101 46-47 0.3743889025259435 1101 48-49 -0.05394648236253374 1101 50-51 -0.39677500968793566 1101 52-53 -0.7629381284370922 1101 54-55 -0.9143946812736274 1101 56-57 -0.6678834477359032 1101 58-59 -0.9696731291830005 1101 60-61 -1.2084157294256457 1101 62-63 -0.9375761951719568 1101 64-65 -0.741297902869178 1101 66-67 -0.9668144853682676 1101 68-69 -1.0917974527491836 1101 70-71 -0.7916453273760169 1101 72-73 -1.185162556956005 1101 74-75 -1.5835899220503613 1101 76-77 -1.4253759304243339 1101 78-79 -1.506148707292855 1101 80-81 -0.3782692551991893 1101 82-83 -0.22994663381225777 1101 84-85 -0.39456781747291814 1101 86-87 -1.1005730994565077 1101 88-89 -1.140880097434792 1101 90-91 -1.1041279303241183 1101 92-93 -0.7842168742612259 1101 94-95 -0.8197319101173655 1101 96-97 -0.8118954382442354 1101 98-99 -1.367880181128026 1101 100-101 -1.0530656935032106 1102 1 0.7879022411156775 1102 2 0.229505512103362 1102 3 0.12934689819574174 1102 4 0.30848638502884995 1102 5 0.3031846983734994 1102 6 0.5302581457856661 1102 7 0.5966737119199337 1102 8 0.10886278492331058 1102 9 0.7296552032922037 1102 10-11 0.17182276162651533 1102 12-13 0.5672024162736236 1102 14-15 0.31729787901966233 1102 16-17 0.714773474733903 1102 18-19 0.65405200131935 1102 20-21 0.1791477650849984 1102 22-23 0.5469181492908248 1102 24-25 0.42228102138324886 1102 26-27 0.8582616039615445 1102 28-29 0.7280519477371996 1102 30-31 0.6068138546003325 1102 32-33 0.9166826751634005 1102 34-35 1.1052389468139978 1102 36-37 0.8971716646917898 1102 38-39 1.2191018004315666 1102 40-41 1.5449661531754018 1102 42-43 1.0617285240757113 1102 44-45 1.4464161575496206 1102 46-47 1.666518532155571 1102 48-49 1.3777664806004282 1102 50-51 0.9909564717935453 1102 52-53 0.22710816785920684 1102 54-55 0.6150034668745192 1102 56-57 1.0365147004122477 1102 58-59 1.2817157597058824 1102 60-61 0.9140379742780524 1102 62-63 0.6582571381613747 1102 64-65 1.0857854304641563 1102 66-67 1.01698181092803 1102 68-69 0.5007951398434081 1102 70-71 1.1657620800313921 1102 72-73 0.9720133689699182 1102 74-75 0.3895582260977868 1102 76-77 0.8468462917978918 1102 78-79 0.3813512927071443 1102 80-81 0.8997400040600674 1102 82-83 0.7638033661877408 1102 84-85 0.10543218252708542 1102 86-87 -0.09571198834539629 1102 88-89 -0.3519912085459005 1102 90-91 -0.14996126365745255 1102 92-93 -0.11685576315011659 1102 94-95 0.04161068247522692 1102 96-97 -0.058423216022013946 1102 98-99 -0.7396394403872861 1102 100-101 -0.6262138416513565 1103 1 -0.2156188856448864 1103 2 -0.1972354894615833 1103 3 0.10313406564488048 1103 4 0.21498090772055178 1103 5 -0.03631060679082054 1103 6 0.27967129132557034 1103 7 0.09197887623918177 1103 8 0.1677438490860652 1103 9 -0.27934323176257436 1103 10-11 0.10751329370788909 1103 12-13 0.12344263536595435 1103 14-15 0.2829179885658277 1103 16-17 0.1042003135445384 1103 18-19 0.35989120319728585 1103 20-21 0.6373832893416491 1103 22-23 0.4110613417790816 1103 24-25 0.5094289087071928 1103 26-27 0.526542120393465 1103 28-29 0.7123535909296876 1103 30-31 0.5186878765095599 1103 32-33 0.44871514777685206 1103 34-35 0.3605206369548455 1103 36-37 0.5834490512332664 1103 38-39 0.4597610336084017 1103 40-41 0.0916699872912119 1103 42-43 0.6255978511492586 1103 44-45 0.16003118102379688 1103 46-47 0.7855521784779498 1103 48-49 0.5897676543093553 1103 50-51 0.9943308849390817 1103 52-53 0.7249074636338548 1103 54-55 0.7480727157008147 1103 56-57 0.7769783154357199 1103 58-59 0.38940355313311414 1103 60-61 0.734020368644245 1103 62-63 0.807024743795175 1103 64-65 0.9427396558162684 1103 66-67 1.3146148312723227 1103 68-69 0.9123757345225947 1103 70-71 1.1829764775274825 1103 72-73 0.9102469370450343 1103 74-75 0.4543078348614813 1103 76-77 0.5060794686367025 1103 78-79 0.6019107606257705 1103 80-81 0.4446832747955938 1103 82-83 0.3993178419310901 1103 84-85 0.98336645482755 1103 86-87 0.7495051478048431 1103 88-89 1.1910447851943182 1103 90-91 1.6817093153722844 1103 92-93 1.2996641899015273 1103 94-95 0.10455082332029875 1103 96-97 0.8367743583128835 1103 98-99 1.3119059430242928 1103 100-101 0.584466909522348 1104 1 -0.1753844721710358 1104 2 -0.05230295720510725 1104 3 -0.3626888438400009 1104 4 -0.12255479601233077 1104 5 -0.12761366992486955 1104 6 -0.12912413859662308 1104 7 -0.2518111365649105 1104 8 -0.17658787552735333 1104 9 -0.323763600126604 1104 10-11 -0.5751420790883301 1104 12-13 0.1930279795991865 1104 14-15 0.22854982152160375 1104 16-17 0.20532805903848583 1104 18-19 0.17583230434966168 1104 20-21 -0.36256746413022967 1104 22-23 0.05638784626052029 1104 24-25 0.14736843397066224 1104 26-27 -0.08266205571212026 1104 28-29 -0.07869339025813105 1104 30-31 0.4266953619818423 1104 32-33 0.5934260706567969 1104 34-35 0.6655536321286846 1104 36-37 0.8428058915760133 1104 38-39 0.16551302809279633 1104 40-41 0.1363365882958405 1104 42-43 0.9291042039513897 1104 44-45 1.325204036337496 1104 46-47 1.6661785943156389 1104 48-49 1.5057530773370331 1104 50-51 1.5555689426560093 1104 52-53 1.0948966293976738 1104 54-55 0.7754784085994615 1104 56-57 1.2031085232560699 1104 58-59 1.4697985102886335 1104 60-61 1.7896935686836457 1104 62-63 1.690162732566968 1104 64-65 0.7437836822124098 1104 66-67 1.0283940098402269 1104 68-69 0.8170344560827196 1104 70-71 1.490742655011971 1104 72-73 0.9570803852869396 1104 74-75 1.3465318080713686 1104 76-77 1.4189131138647113 1104 78-79 1.3387619376177895 1104 80-81 0.867858690928756 1104 82-83 0.5426252387596122 1104 84-85 0.7720988491937497 1104 86-87 0.6998154009319961 1104 88-89 0.5819637254090324 1104 90-91 0.04793112173493341 1104 92-93 0.21757022752587218 1104 94-95 0.37006347967802355 1104 96-97 0.12205949571069752 1104 98-99 -0.10041061365846105 1104 100-101 0.5290891886516711 1105 1 -0.2537644255509903 1105 2 -0.3052167101188594 1105 3 -0.05120865735981539 1105 4 0.07931971836217855 1105 5 0.01151803170682797 1105 6 -0.0530751875476696 1105 7 0.12445148969771225 1105 8 0.16441834047886772 1105 9 0.2921552032922037 1105 10-11 0.2204338727376225 1105 12-13 0.20956352738473782 1105 14-15 0.21313121235299803 1105 16-17 0.4890790302894601 1105 18-19 0.0672464457637929 1105 20-21 0.20345332064055555 1105 22-23 -0.10585962848696084 1105 24-25 0.09241991027213459 1105 26-27 0.11173382618375882 1105 28-29 0.2870797255149782 1105 30-31 0.22139718793366114 1105 32-33 0.14237711960784338 1105 34-35 0.014961169036219246 1105 36-37 0.5499494424695683 1105 38-39 0.479518467098238 1105 40-41 0.7706605976198446 1105 42-43 0.9089507462979256 1105 44-45 0.34919393532739207 1105 46-47 0.5449907543777996 1105 48-49 0.9402664806004353 1105 50-51 1.0985953606824381 1105 52-53 1.1680803900814354 1105 54-55 1.0177812446522978 1105 56-57 0.8385980337455763 1105 58-59 0.8442157597058824 1105 60-61 0.24042686316693818 1105 62-63 0.4881182492724889 1105 64-65 0.2524520971308277 1105 66-67 0.49267625537247284 1105 68-69 0.3584340287323009 1105 70-71 0.36020652447583856 1105 72-73 0.4338189245254753 1105 74-75 0.3062248927644511 1105 76-77 0.18365184735344542 1105 78-79 0.29454573715158716 1105 80-81 0.26085111517118165 1105 82-83 0.21866447729884797 1105 84-85 0.6089044047493068 1105 86-87 0.9945657894323823 1105 88-89 0.24523101367632094 1105 90-91 0.35003873634254745 1105 92-93 0.6088386812943263 1105 94-95 0.5381384602530055 1105 96-97 0.10824345064465035 1105 98-99 0.05897167072382459 1105 100-101 0.995313936126422 1106 1 -0.04599037633398595 1106 2 -0.236984047926466 1106 3 0.054216398568598834 1106 4 -0.05933613547824024 1106 5 0.2496332442347935 1106 6 -0.11096109358793882 1106 7 -0.4256976527333052 1106 8 0.06253541728721501 1106 9 -0.04756329036924711 1106 10-11 -0.23032582152038827 1106 12-13 -0.3184705889463615 1106 14-15 -0.06555540211382294 1106 16-17 -0.49493476538540904 1106 18-19 -0.10482663402739689 1106 20-21 -0.358930161835211 1106 22-23 -0.028725027442966677 1106 24-25 -0.44642703230802994 1106 26-27 -0.2943530492823072 1106 28-29 -0.37716337664758015 1106 30-31 -0.3073826405525395 1106 32-33 -0.22339469247269506 1106 34-35 -0.33519729479674254 1106 36-37 -0.11711338377949687 1106 38-39 -0.38280442626493283 1106 40-41 0.21389325981223806 1106 42-43 -0.16426886593175283 1106 44-45 -0.02897534133181523 1106 46-47 -0.32513881310915593 1106 48-49 -0.32364571179330426 1106 50-51 -0.2586939756337472 1106 52-53 -0.4538901542884446 1106 54-55 -0.45707427361764985 1106 56-57 -0.5078784763215367 1106 58-59 -0.33223837899657127 1106 60-61 0.14495240380825436 1106 62-63 0.22662962138284826 1106 64-65 0.14304214187355058 1106 66-67 -0.06127135834862685 1106 68-69 0.10743197802387172 1106 70-71 0.29756670344675484 1106 72-73 0.6544099014083962 1106 74-75 0.7034098293789199 1106 76-77 0.08155919261817601 1106 78-79 0.27699819800169934 1106 80-81 0.2003785200928796 1106 82-83 0.03657089042338413 1106 84-85 0.1915619364420742 1106 86-87 0.2848109423033769 1106 88-89 0.5656075983146494 1106 90-91 0.25612561180861704 1106 92-93 0.590428912465093 1106 94-95 0.5829277965691837 1106 96-97 0.44798524781094784 1106 98-99 1.2197658541690188 1106 100-101 1.5245132276998703 1107 1 0.29286912853289593 1107 2 0.2632159609628175 1107 3 0.026881850366454074 1107 4 0.04009050570581252 1107 5 0.33781494119910604 1107 6 0.0735804415914032 1107 7 0.33398055518704695 1107 8 0.13360524261278783 1107 9 0.07053084714797819 1107 10-11 0.3454108778884688 1107 12-13 0.11057070177766803 1107 14-15 0.1076078495862518 1107 16-17 0.780699707257817 1107 18-19 0.410329595138343 1107 20-21 0.3960811720018498 1107 22-23 0.5271885687168663 1107 24-25 0.9301452597938464 1107 26-27 0.5361727518644059 1107 28-29 0.7482644201433786 1107 30-31 0.17170531891232343 1107 32-33 0.3940327487469162 1107 34-35 0.387523715025921 1107 36-37 0.10194079640628928 1107 38-39 -0.2700216359186882 1107 40-41 0.09157671241013077 1107 42-43 0.24223808993294682 1107 44-45 0.9121998220087733 1107 46-47 1.5330104379686702 1107 48-49 1.1087957300485556 1107 50-51 0.7017042642880256 1107 52-53 0.15322571752808045 1107 54-55 0.43198746245951014 1107 56-57 0.557968894672733 1107 58-59 0.9819089164387762 1107 60-61 0.7482681067283821 1107 62-63 1.0990683964395203 1107 64-65 0.8231612582787307 1107 66-67 0.7128519360200158 1107 68-69 1.159367619607945 1107 70-71 1.5312882021800363 1107 72-73 1.1173638104710228 1107 74-75 1.0092234210941093 1107 76-77 1.263145040878097 1107 78-79 1.1206356930014785 1107 80-81 1.1475095036921523 1107 82-83 1.210156383111947 1107 84-85 1.6230922266771906 1107 86-87 1.130626311875357 1107 88-89 1.6951022425210596 1107 90-91 1.9520898768870687 1107 92-93 2.1463202854150047 1107 94-95 2.2450461129388017 1107 96-97 1.8643370856704067 1107 98-99 1.436731052622278 1107 100-101 1.655519050180871 1108 1 0.020419723633160913 1108 2 -0.05929596419811389 1108 3 -0.25080073195188746 1108 4 -0.25542192887946413 1108 5 -0.04369758600878981 1108 6 0.12262411315163035 1108 7 0.2866504018966225 1108 8 0.12411142517194662 1108 9 0.6902224138594093 1108 10-11 0.24303973909348997 1108 12-13 0.5286923152635197 1108 14-15 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-2.617260415712149 2316 70-71 -2.8153887136194022 2316 72-73 -2.6485223453157936 2316 74-75 -2.5052830437434856 2316 76-77 -3.0301378351862347 2316 78-79 -2.486704262848413 2316 80-81 -2.6483750753050117 2316 82-83 -2.494629173494797 2316 84-85 -2.7219487698538707 2316 86-87 -2.207319131202535 2316 88-89 -2.3480229545776474 2316 90-91 -2.6241676128638005 2316 92-93 -2.7969152869596385 2316 94-95 -2.6116631270485833 2316 96-97 -2.6382843271331247 2316 98-99 -2.9023378530856974 2316 100-101 -2.837523365460882 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1568.0 3 281.0 4 241.0 5 247.0 6 255.0 7 307.0 8 343.0 9 407.0 10 462.0 11 541.0 12 722.0 13 891.0 14 1000.0 15 1129.0 16 1455.0 17 1870.0 18 1590.0 19 1052.0 20 994.0 21 1074.0 22 1170.0 23 1366.0 24 1432.0 25 1605.0 26 1904.0 27 2136.0 28 2516.0 29 2996.0 30 3507.0 31 4301.0 32 5098.0 33 6489.0 34 9102.0 35 13015.0 36 17521.0 37 25637.0 38 24841.0 39 4176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47574659071293 20.32867253581909 14.4360435007768 20.759537372691177 2 14.809757308671095 22.377395706177282 40.634073338436814 22.178773646714806 3 19.548540206413158 28.824024504663615 29.23174568132899 22.39568960759424 4 11.591404375051722 17.42641582301178 39.60718325011724 31.37499655181926 5 12.214462188237569 39.85383224524161 33.561303501676996 14.370402064843827 6 28.078114329468086 38.12729793677182 18.707016079521548 15.087571654238552 7 26.812619489671263 34.88787813951562 21.84184916521151 16.45765320560161 8 25.616052569819292 33.23025525629168 21.159076162734515 19.99461601115452 9 27.509739700051718 17.80865368039993 21.93759696604034 32.744009653508016 10-11 23.83915881273372 27.81706660779092 27.345140680842846 20.99863389863252 12-13 26.49626021196732 25.815232391256348 27.771031132700376 19.917476264075955 14-15 23.439930199916542 26.411261815836756 28.13591703997986 22.012890944266843 16-17 20.246434502211283 29.969515037061846 28.54435165077973 21.239698809947143 18-19 19.878674110932444 28.64236468160581 32.0267910751627 19.45217013229905 20-21 21.500075890331573 27.41572723635009 31.20575938625419 19.878437487064147 22-23 20.626188512284603 27.345359668407234 31.18725259099992 20.84119922830825 24-25 20.736643420830156 27.671402100061766 31.55406641108899 20.03788806801909 26-27 20.75551870660172 27.118872422012647 32.25031954952154 19.875289321864095 28-29 19.81931580365098 27.662432744175767 32.563560639025305 19.954690813147952 30-31 20.356597819256557 28.040990795025312 31.74473286238114 19.857678523336993 32-33 19.73433226059032 28.335247713953592 31.567619027908567 20.362800997547524 34-35 20.583245512499353 27.79054299189745 31.690133545255083 19.936077950348118 36-37 20.640758844311087 27.326622465276095 31.69675264966365 20.335866040749163 38-39 20.36628061698826 27.856750339564734 31.975074393190045 19.801894650256962 40-41 19.994541352708076 27.97591285511437 32.08440865230144 19.94513713987611 42-43 19.595082652768514 28.377047310631326 31.071081726532178 20.95678831006798 44-45 19.767317270436642 28.337728681982988 30.850162967776274 21.044791079804092 46-47 20.419555764484663 28.263845201966088 30.439396348502967 20.877202685046285 48-49 20.432921685497366 28.430321804428093 30.1793641354704 20.95739237460414 50-51 20.316032550453894 28.690100043557322 30.23154517861958 20.762322227369207 52-53 20.392232781478704 28.980492071338936 29.830917456882393 20.796357690299967 54-55 19.816235312825157 28.58757687109163 30.241320782762898 21.354867033320314 56-57 20.59043089981614 28.91639133500145 29.24367889185341 21.249498873329 58-59 20.65411551710437 28.322317390448088 29.781397164762343 21.2421699276852 60-61 20.527353801624063 28.598616335257198 30.019929469710316 20.854100393408423 62-63 20.7729902736781 28.52190323450321 30.219689061862727 20.485417429955966 64-65 20.320019914808874 28.516554184875808 30.354179343917686 20.809246556397632 66-67 20.359248310080737 28.612278063032694 29.662361910689278 21.36611171619729 68-69 20.116870094395075 28.805366342795892 29.927734172400676 21.150029390408353 70-71 20.56714291641886 28.410741206898937 29.65934081133041 21.362775065351794 72-73 20.38377761642983 28.224596342011548 29.586142516336476 21.80548352522214 74-75 20.151919041049947 29.040254742716083 29.37009262428561 21.43773359194836 76-77 20.397122521008985 28.112112444301562 29.951500444204772 21.539264590484684 78-79 20.118541558665974 28.222913426645928 30.228788664247453 21.42975635044064 80-81 20.27650088134656 29.153907303079528 29.29803338748142 21.27155842809249 82-83 20.45573173218847 28.448320560815503 29.364346873239246 21.73160083375678 84-85 20.244255704542546 28.21419559122947 29.348709067465872 22.19283963676211 86-87 20.4578448180511 28.466845481694502 29.571880876009292 21.503428824245105 88-89 20.82327970630734 27.880753036829116 29.368574608859177 21.92739264800437 90-91 20.462065603357964 27.914100884092896 29.653934853037278 21.969898659511863 92-93 20.952143862305988 28.431545427199268 29.239923795298406 21.37638691519634 94-95 20.637824081437888 27.93134210690106 29.35283988354161 22.077993928119447 96-97 20.3670086409892 27.867968174603995 29.31919793363139 22.445825250775407 98-99 20.83454351824959 27.930722100051174 28.90682267679073 22.32791170490851 100-101 21.32158498940506 27.002755030436692 29.66860129489887 22.007058685259377 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 619.0 1 389.5 2 208.5 3 253.5 4 207.5 5 184.5 6 321.5 7 605.0 8 843.5 9 825.0 10 622.0 11 484.0 12 459.0 13 442.5 14 399.5 15 363.0 16 362.5 17 369.5 18 383.0 19 405.5 20 427.5 21 449.0 22 500.0 23 555.5 24 608.5 25 721.0 26 845.0 27 1010.5 28 1155.0 29 1308.5 30 1703.0 31 2122.5 32 2629.0 33 3278.0 34 3691.0 35 4148.0 36 5023.5 37 5600.0 38 5486.0 39 5351.5 40 5043.0 41 4653.0 42 4251.0 43 4286.0 44 4604.5 45 4653.0 46 4605.5 47 4876.0 48 6844.0 49 6886.5 50 4867.0 51 3913.0 52 3311.0 53 3112.5 54 3052.5 55 2903.5 56 2623.0 57 2380.0 58 2213.5 59 2033.5 60 1798.0 61 1573.5 62 1307.5 63 986.5 64 769.0 65 609.0 66 452.5 67 362.5 68 272.5 69 185.0 70 148.5 71 123.0 72 93.0 73 91.0 74 78.0 75 49.0 76 43.0 77 34.0 78 25.5 79 17.0 80 13.0 81 12.0 82 9.5 83 7.5 84 6.5 85 3.0 86 1.0 87 2.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28642050109817474 2 0.16661961842730358 3 0.19897962696483776 4 0.1631770643275659 5 0.23340516796221453 6 0.1886519646656247 7 0.24373283026142756 8 0.2526834709207455 9 0.14871833710866766 10-11 0.20861877844410323 12-13 0.2155038866435786 14-15 0.17591451449659531 16-17 0.286076245688201 18-19 0.23547070042205714 20-21 0.20311069188452294 22-23 0.25096219387087665 24-25 0.23443793419213582 26-27 0.348386474893453 28-29 0.31533795553597127 30-31 0.2946826309375452 32-33 0.19656983909502138 34-35 0.36732052244201013 36-37 0.4131064919685213 38-39 0.39279542278006896 40-41 0.35561583850290207 42-43 0.39210691196012143 44-45 0.39968053097954437 46-47 0.4048443621291508 48-49 0.42618819754752446 50-51 0.4158605352483114 52-53 0.41827032311812784 54-55 0.4127622365585475 56-57 0.39038563491025263 58-59 0.3625009467023774 60-61 0.33082944898479083 62-63 0.40071329720946564 64-65 0.4303192624672097 66-67 0.4361716044367637 68-69 0.4378928814866325 70-71 0.4399584139464752 72-73 0.4310077732871572 74-75 0.42997500705723596 76-77 0.413450747378495 78-79 0.3896971240903051 80-81 0.39658223228978046 82-83 0.40931968245880984 84-85 0.41138521491865243 86-87 0.4027788296693082 88-89 0.4131064919685213 90-91 0.4334175611569736 92-93 0.43410607197692114 94-95 0.44064692476642275 96-97 0.44030266935644896 98-99 0.437204370666685 100-101 0.4106967040987049 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 145241.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.05272615859159 #Duplication Level Percentage of deduplicated Percentage of total 1 85.4581363868362 53.029103352359186 2 8.01433548587533 9.946227304962099 3 2.3156469831125315 4.3107662436915195 4 1.1073386148281295 2.7485351932305617 5 0.6579677340612032 2.0414345811444425 6 0.4327275148125957 1.611115318677233 7 0.3495106850409427 1.5181663579843159 8 0.2563078356966913 1.2723679952630456 9 0.16532410181301732 0.9232930095496451 >10 1.1417349044670795 12.600436515859847 >50 0.05991611743559017 2.5647028043045696 >100 0.03439628963894992 3.9919857340558105 >500 0.005547788651443534 2.421492553755482 >1k 0.0011095577302887068 1.0203730351622475 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1482 1.0203730351622475 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 953 0.6561508114100013 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 798 0.5494316343181334 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 608 0.4186145785281016 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 599 0.41241798114857375 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 559 0.38487754835067234 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 439 0.3022562499569681 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 359 0.24717538436116523 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 310 0.21343835418373597 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 291 0.20035664860473282 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 287 0.19760260532494267 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 268 0.18452089974593952 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 243 0.1673081292472511 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 243 0.1673081292472511 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 228 0.15698046694803808 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 208 0.14321025054908737 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 197 0.1356366315296645 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 191 0.13150556660997928 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 174 0.11980088267087116 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 168 0.11566981775118597 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 158 0.1087847095517106 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.4425540997376773E-4 0.0 20-21 0.0 0.0 0.0 0.0017212770498688387 0.0 22-23 0.0 0.0 0.0 0.004131064919685213 0.0 24-25 0.0 0.0 0.0 0.00619659737952782 0.0 26-27 0.0 0.0 0.0 0.007917874429396659 0.0 28-29 0.0 0.0 0.0 0.019278302958530995 0.0 30-31 0.0 0.0 0.0 0.04544171411653734 0.0 32-33 0.0 0.0 0.0 0.07573619019422889 0.0 34-35 0.0 0.0 0.0 0.11911237185092363 0.0 36-37 0.0 0.0 0.0 0.1876191984357034 0.0 38-39 0.0 0.0 0.0 0.2833222024084109 0.0 40-41 0.0 0.0 0.0 0.3993362755695706 0.0 42-43 0.0 0.0 0.0 0.514317582500809 0.0 44-45 0.0 0.0 0.0 0.6451346382908407 0.0 46-47 0.0 0.0 0.0 0.8010823390089575 0.0 48-49 0.0 0.0 0.0 0.9769968535055529 0.0 50-51 0.0 0.0 0.0 1.1577309437417809 0.0 52-53 0.0 0.0 0.0 1.3374322677480877 0.0 54-55 0.0 0.0 0.0 1.5099042281449453 0.0 56-57 0.0 0.0 0.0 1.7484732272567665 0.0 58-59 0.0 0.0 0.0 2.0063205293271182 0.0 60-61 0.0 0.0 0.0 2.303757203544454 0.0 62-63 0.0 0.0 0.0 2.6225377131801624 0.0 64-65 0.0 0.0 0.0 2.9251382185471044 0.0 66-67 0.0 0.0 0.0 3.2556234121219214 0.0 68-69 0.0 0.0 0.0 3.6573694755613086 0.0 70-71 0.0 0.0 0.0 4.126933854765527 0.0 72-73 0.0 0.0 0.0 4.586170571670534 0.0 74-75 0.0 0.0 0.0 5.0543579292348575 0.0 76-77 0.0 0.0 0.0 5.533905715328316 0.0 78-79 0.0 0.0 0.0 6.069567133247499 0.0 80-81 0.0 0.0 0.0 6.68819410497036 0.0 82-83 0.0 0.0 0.0 7.317492994402407 0.0 84-85 0.0 0.0 0.0 7.937152732355189 0.0 86-87 0.0 0.0 0.0 8.559566513587761 0.0 88-89 0.0 0.0 0.0 9.211586260078079 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGTGG 70 4.3511463E-6 40.622246 8 AGTAGTG 50 0.0016291506 37.914097 7 CTGTTAG 65 1.3265546E-4 36.51911 1 CTACCTC 55 0.0025788662 34.52716 1 TCCCCGG 55 0.0026008957 34.46736 6 CTATACA 60 0.0039767493 31.606031 4 TATTAAG 60 0.0039767493 31.606031 2 GGCTAGG 65 0.0058424496 29.21529 1 CTCCCCG 65 0.005882207 29.174795 5 TAAGTAG 65 0.005882207 29.174795 5 AAGTAGT 65 0.005892179 29.16469 6 TAGACGA 65 0.005892179 29.16469 9 AGTATGA 65 0.005892179 29.16469 6 CATGGGT 100 4.988309E-5 28.445427 4 CATGGGG 235 0.0 28.243685 4 TACCTCC 70 0.008446298 27.090883 2 AGTACTC 70 0.008446298 27.090883 5 TAGTGGG 70 0.008460592 27.081497 9 CACAGGG 95 0.0012375843 24.943487 7 GTACATG 1215 0.0 24.616587 1 >>END_MODULE