FastQCFastQC Report
Thu 26 May 2016
SRR938732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145241
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.9177849229900648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9640.6637244304294242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.5576937641575037No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6860.47231842248400935No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6080.4186145785281016No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5380.3704188211317741No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4310.2967481633973878No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3260.22445452730289656No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.22238899484305397No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.20724175680420817No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT3000.20655324598426064No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2930.20173367024462788No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.16042302104777578No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2310.1590459994078807No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2100.14458727218898246No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.1438987613690349No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT1930.13288258824987434No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1930.13288258824987434No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1790.12324343677060885No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1750.12048939349081872No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1660.11429279611129088No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.11085024201155322No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.10534215545197292No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1500.10327662299213032No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC1490.10258811217218279No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG1470.1012110905322877No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCC250.0050390256.9239654
GTCGGGA558.216157E-751.749062
GGTCGGG652.5004556E-644.030261
CGGGAGT704.323654E-640.6599774
TCGGGAG801.0809868E-535.577483
CCTACAC753.089408E-431.6244283
TACCTGG753.089408E-431.6244282
CGCTGCA300.003972002331.62442624-25
ACTATAC650.00586562429.1917783
ACAGCAC650.00586562429.1917788
CTACAGT650.00586562429.1917784
AGTAGCT856.430038E-427.9039067
ATGGGGT1056.9277194E-527.1066517
CTATGAC700.00842252827.1066513
ACTGATA1056.9277194E-527.1066518
AAGGTAG350.00843617527.10665160-61
GCCGCAC350.00843617527.10665146-47
GGACAAT700.00842252827.1066518
GTGATCT458.584031E-426.56869184-85
GGAGTAA950.001191893225.104971
Produced by FastQC (version 0.11.5)