##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938732_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 145241 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00204487713525 31.0 31.0 34.0 30.0 34.0 2 32.1955233026487 33.0 31.0 34.0 30.0 34.0 3 32.265579278578365 34.0 31.0 34.0 30.0 34.0 4 35.89418965719046 37.0 35.0 37.0 35.0 37.0 5 35.8123188355905 37.0 35.0 37.0 35.0 37.0 6 35.86496925799189 37.0 35.0 37.0 35.0 37.0 7 35.778444103249086 37.0 35.0 37.0 33.0 37.0 8 35.76781349618909 37.0 35.0 37.0 33.0 37.0 9 37.38161400706412 39.0 37.0 39.0 34.0 39.0 10-11 37.3019429775339 39.0 37.0 39.0 34.0 39.0 12-13 37.246486873541215 39.0 37.0 39.0 33.0 39.0 14-15 38.37635722695382 40.0 38.0 41.0 33.0 41.0 16-17 38.32655035423882 40.0 38.0 41.0 33.0 41.0 18-19 38.50494350768722 40.0 38.0 41.0 34.0 41.0 20-21 38.53212247230465 40.0 38.0 41.0 34.0 41.0 22-23 38.44735302015271 40.0 38.0 41.0 34.0 41.0 24-25 38.346610116977985 40.0 38.0 41.0 34.0 41.0 26-27 38.13871083234073 40.0 37.0 41.0 33.0 41.0 28-29 37.94656123270977 40.0 37.0 41.0 33.0 41.0 30-31 37.68257585667959 40.0 36.5 41.0 33.0 41.0 32-33 37.45826247409478 40.0 36.0 41.0 32.0 41.0 34-35 37.37851570837436 40.0 36.0 41.0 32.0 41.0 36-37 37.21171707713387 40.0 35.5 41.0 31.5 41.0 38-39 37.06087468414566 40.0 35.0 41.0 31.0 41.0 40-41 36.86206718488582 39.5 35.0 41.0 31.0 41.0 42-43 36.64238403756515 39.0 35.0 41.0 30.5 41.0 44-45 36.31409175095186 39.0 35.0 41.0 30.0 41.0 46-47 35.890509566857844 39.0 35.0 41.0 28.5 41.0 48-49 35.528821062923 39.0 35.0 41.0 27.0 41.0 50-51 35.29357068596333 39.0 35.0 40.5 27.0 41.0 52-53 35.06487493200956 38.5 35.0 40.0 26.0 41.0 54-55 34.801908551992895 38.0 34.0 40.0 25.5 41.0 56-57 34.5524920649128 38.0 34.0 40.0 24.5 41.0 58-59 34.27454024689998 38.0 34.0 40.0 23.5 41.0 60-61 33.90538484312281 37.5 33.0 40.0 23.0 41.0 62-63 33.53690762250329 37.0 33.0 40.0 22.0 41.0 64-65 33.1442739997659 36.0 33.0 39.0 21.5 41.0 66-67 32.745092639130824 36.0 32.0 39.0 20.0 40.0 68-69 32.436708642876326 35.5 32.0 39.0 19.5 40.0 70-71 31.995899918067213 35.0 31.5 38.0 17.5 40.0 72-73 31.47353020152712 35.0 31.0 37.0 13.0 39.5 74-75 30.966631323111244 35.0 31.0 37.0 8.0 39.0 76-77 29.006255120799224 32.5 28.0 35.0 6.5 37.5 78-79 29.959642938288773 34.0 29.5 36.0 2.0 38.0 80-81 29.997576441913786 34.0 30.0 35.5 2.0 37.0 82-83 29.651871716664026 34.0 30.0 35.0 2.0 37.0 84-85 29.235243491851474 34.0 29.0 35.0 2.0 36.5 86-87 28.653482831982707 34.0 29.0 35.0 2.0 36.0 88-89 28.038346610116978 34.0 28.0 35.0 2.0 35.5 90-91 27.49935968493745 33.0 26.5 35.0 2.0 35.0 92-93 27.19762670320364 33.0 26.0 35.0 2.0 35.0 94-95 26.837511446492382 33.0 25.5 35.0 2.0 35.0 96-97 26.237185092363728 33.0 25.0 35.0 2.0 35.0 98-99 25.4304431944148 32.5 22.0 34.5 2.0 35.0 100-101 21.825366115628505 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.005921994509108686 1101 2 0.1828480233983143 1101 3 -0.1442023923818283 1101 4 0.31189182400065363 1101 5 0.15369949089277668 1101 6 0.3785942444405066 1101 7 -0.11895501248746143 1101 8 0.279980537521908 1101 9 0.19563772458396045 1101 10-11 0.48425845305679616 1101 12-13 0.16744478170434007 1101 14-15 0.40259647849489255 1101 16-17 0.14106689962770247 1101 18-19 0.4477952874821014 1101 20-21 0.6077681286767955 1101 22-23 0.5410616828343393 1101 24-25 0.5950338754565152 1101 26-27 0.506222600248087 1101 28-29 0.5197203146129326 1101 30-31 1.0158261809655968 1101 32-33 0.6688672996179434 1101 34-35 0.5026371993611107 1101 36-37 0.4718018589334463 1101 38-39 0.6678449498755299 1101 40-41 0.7777759715740515 1101 42-43 0.8436927394809501 1101 44-45 0.34041176381352045 1101 46-47 0.10513493292991427 1101 48-49 -0.14257355081714707 1101 50-51 -0.5220356647068343 1101 52-53 -0.17479425841558083 1101 54-55 0.1023289845439237 1101 56-57 0.8015186844133595 1101 58-59 0.4140597385346467 1101 60-61 0.12415207929757344 1101 62-63 0.011035587739108621 1101 64-65 -0.2574278552777116 1101 66-67 -0.1119385290908852 1101 68-69 2.91842119743535E-4 1101 70-71 0.10453334146525606 1101 72-73 -0.27172348184371486 1101 74-75 -0.23127810388854186 1101 76-77 -0.7108832917902923 1101 78-79 -0.48263789928862266 1101 80-81 -0.02909441830182402 1101 82-83 0.16260702610190947 1101 84-85 0.15288161016071555 1101 86-87 0.4105648345161832 1101 88-89 0.26959444259889054 1101 90-91 0.4362933893890144 1101 92-93 1.037204103128385 1101 94-95 0.7780142680874143 1101 96-97 0.8530324121797754 1101 98-99 0.28259450750643467 1101 100-101 0.40269514367030723 1102 1 0.1443479204350382 1102 2 -0.14817049512020475 1102 3 0.13774205206261314 1102 4 0.2086510832599089 1102 5 0.26018097237425764 1102 6 -0.048257607411343884 1102 7 0.25558202454957524 1102 8 0.400813870855238 1102 9 0.09424883569507614 1102 10-11 -0.06643599138764245 1102 12-13 0.13596330022286196 1102 14-15 0.11880018219859778 1102 16-17 0.22972430703510582 1102 18-19 0.5306656578524738 1102 20-21 0.3436477583064246 1102 22-23 0.5306450161676679 1102 24-25 0.28461720878984664 1102 26-27 0.605759637285118 1102 28-29 0.3539795738721878 1102 30-31 0.08110395874337684 1102 32-33 0.3857654477660901 1102 34-35 0.3456927549166693 1102 36-37 0.3109222293038201 1102 38-39 0.45765976469034797 1102 40-41 0.40300745305552965 1102 42-43 0.18350755429576537 1102 44-45 -0.17139379174204095 1102 46-47 -0.3129206226256471 1102 48-49 -0.46826799526159135 1102 50-51 0.03676063158945908 1102 52-53 0.11478907491775203 1102 54-55 0.22848639195132847 1102 56-57 0.4575372029318743 1102 58-59 0.6166060348309514 1102 60-61 -0.035107179961684665 1102 62-63 -0.16141811596460087 1102 64-65 -0.09284452194437165 1102 66-67 0.09431147090911196 1102 68-69 -0.21336556528765982 1102 70-71 0.07675556368747749 1102 72-73 0.01670244408221322 1102 74-75 -0.08475032611076472 1102 76-77 0.0023111526541512717 1102 78-79 0.12453802663729974 1102 80-81 0.32646113725373027 1102 82-83 0.3072829520278333 1102 84-85 0.6714001286792346 1102 86-87 0.4175092789606296 1102 88-89 -0.06860000184555659 1102 90-91 0.01129338938901725 1102 92-93 0.02076891794319735 1102 94-95 -0.06365239857925431 1102 96-97 -0.3198842544868903 1102 98-99 -0.19772956656763796 1102 100-101 1.0327877362629039 1103 1 0.061894868791846136 1103 2 0.08529977092048568 1103 3 -0.21525090568386673 1103 4 -0.1842479777729551 1103 5 0.042849203986151 1103 6 -0.07153616766173343 1103 7 -0.44795866402633067 1103 8 -0.11062979111659388 1103 9 -0.21531689200445925 1103 10-11 0.02878114476258986 1103 12-13 -0.08352026785225775 1103 14-15 -0.1827452012129811 1103 16-17 -0.28274635024188655 1103 18-19 -0.059612119925304796 1103 20-21 -0.0027430867639992584 1103 22-23 -0.02857837976347355 1103 24-25 -0.15254045944176653 1103 26-27 -0.09093441592302298 1103 28-29 0.16129569280802514 1103 30-31 -0.1330001101142173 1103 32-33 -0.06694386365801108 1103 34-35 -0.10784793365922951 1103 36-37 -0.2302064874410945 1103 38-39 0.14212768331319126 1103 40-41 0.01700393192876959 1103 42-43 0.051856537081363285 1103 44-45 0.3494884461296337 1103 46-47 0.3513008171239562 1103 48-49 0.05163615184326176 1103 50-51 -0.12589586293323407 1103 52-53 -0.29615576076299277 1103 54-55 -0.17688332635852788 1103 56-57 -0.1740160052058357 1103 58-59 -0.17462049099064814 1103 60-61 -0.13017760249690014 1103 62-63 -0.8202111519583397 1103 64-65 -0.5759724562166753 1103 66-67 0.04447774633946722 1103 68-69 -0.4307462382141054 1103 70-71 -0.4627593032921773 1103 72-73 -0.2738883227409481 1103 74-75 -0.2682890585051325 1103 76-77 -0.27121193028794366 1103 78-79 -0.2842550093564391 1103 80-81 -0.4693135106335937 1103 82-83 -0.6031728382695043 1103 84-85 -1.0926843783630176 1103 86-87 -1.0941300011645652 1103 88-89 -1.2365870910474328 1103 90-91 -0.3914843883887613 1103 92-93 0.32588433265368266 1103 94-95 0.11103461237536294 1103 96-97 -0.17145898062147324 1103 98-99 -0.4354545274440085 1103 100-101 0.617196969439739 1104 1 0.3062068947940091 1104 2 0.22998607053636277 1104 3 0.00793435975491974 1104 4 0.013575325684151096 1104 5 0.11381345100673457 1104 6 0.26535933120559463 1104 7 0.2559219623895075 1104 8 0.2412858988272646 1104 9 0.3985903275365672 1104 10-11 0.37594454474288597 1104 12-13 0.6178980321575906 1104 14-15 0.273277649176066 1104 16-17 0.741015099575904 1104 18-19 0.7480801877670018 1104 20-21 0.6506844715931379 1104 22-23 0.6848068265794751 1104 24-25 0.443441023863663 1104 26-27 0.5518066458321229 1104 28-29 0.9261435210361313 1104 30-31 1.093681658821076 1104 32-33 1.0700846008352443 1104 34-35 0.7009277976517083 1104 36-37 0.7380541251857196 1104 38-39 0.6366613186918926 1104 40-41 1.1466700404681163 1104 42-43 1.0849255807137936 1104 44-45 1.1519113753131265 1104 46-47 0.5378466656416379 1104 48-49 0.12727881903522587 1104 50-51 0.34061649794532656 1104 52-53 0.6065577229364081 1104 54-55 0.4957503779653152 1104 56-57 0.4551576380523059 1104 58-59 0.26047258494749315 1104 60-61 0.33202568717118197 1104 62-63 0.39085946756298284 1104 64-65 -0.269782167632016 1104 66-67 -0.19266890982127194 1104 68-69 0.1699629117908188 1104 70-71 0.1165768535087679 1104 72-73 0.7273182171979826 1104 74-75 0.6982479256374887 1104 76-77 0.5588136779066737 1104 78-79 0.2484939318432069 1104 80-81 0.47681078760338025 1104 82-83 0.6891545526494376 1104 84-85 0.27979173707084115 1104 86-87 0.8522897762411255 1104 88-89 1.540301513305959 1104 90-91 1.5787953318909587 1104 92-93 1.2614206273449078 1104 94-95 1.1909124809856237 1104 96-97 1.7922709514183168 1104 98-99 2.13703636194829 1104 100-101 3.1271447681199334 1105 1 0.06101458710170249 1105 2 0.17127394932424522 1105 3 0.082186496507056 1105 4 -0.12468225007341971 1105 5 -0.07315236095907096 1105 6 0.03507572592199182 1105 7 0.14447091343846097 1105 8 0.4424805375219023 1105 9 0.25397105791729757 1105 10-11 0.12453623083457188 1105 12-13 0.10124107800063342 1105 14-15 0.22296684886526208 1105 16-17 -0.14180347074266564 1105 18-19 0.04455454674136661 1105 20-21 0.25684220275086744 1105 22-23 -0.05963276161010356 1105 24-25 -0.2813550134323819 1105 26-27 -0.13035147382598922 1105 28-29 0.0032851294277449483 1105 30-31 -0.28000715236773743 1105 32-33 0.048959892210532985 1105 34-35 0.390831643805555 1105 36-37 0.3977277848593772 1105 38-39 0.6486319869125623 1105 40-41 0.03495189749997252 1105 42-43 -0.4206591123708989 1105 44-45 0.07166176381351619 1105 46-47 -0.6150039559589828 1105 48-49 -0.2425735508171485 1105 50-51 -0.5083782572994267 1105 52-53 -0.07965536952669083 1105 54-55 0.3673752808402142 1105 56-57 0.25962053626521 1105 58-59 0.2798004792753943 1105 60-61 0.39197615337164393 1105 62-63 -0.2273903381868223 1105 64-65 -0.054650077499928784 1105 66-67 -0.26332741797977377 1105 68-69 -0.13697667639876698 1105 70-71 -0.10727221409030108 1105 72-73 -0.07704755591778678 1105 74-75 -0.16808365944409687 1105 76-77 -0.17477218067918088 1105 78-79 -0.10115641780714313 1105 80-81 0.06604447058706242 1105 82-83 -0.21007815908327387 1105 84-85 -0.09248876020965469 1105 86-87 0.37237039007174033 1105 88-89 -0.01998889073444232 1105 90-91 0.3272656116112387 1105 92-93 0.510352251276533 1105 94-95 0.79051426808741 1105 96-97 1.4058101899575561 1105 98-99 1.8057426556545835 1105 100-101 3.150843291818461 1106 1 -0.1625592384016521 1106 2 -0.14402247125741496 1106 3 -0.08499284726624268 1106 4 -0.24450887199736115 1106 5 0.07664256819827386 1106 6 -0.17735902426444028 1106 7 0.08616554431094414 1106 8 0.05895145474785579 1106 9 0.02979134128791827 1106 10-11 -0.0684633963093475 1106 12-13 0.048155693958875645 1106 14-15 -0.10570578349864235 1106 16-17 -0.1365368786472132 1106 18-19 -0.2475315830125595 1106 20-21 -0.4820653289418928 1106 22-23 -0.30644018144604956 1106 24-25 -0.7258460648865181 1106 26-27 -0.4474189607760266 1106 28-29 -0.6164715819816493 1106 30-31 -0.3181782933073336 1106 32-33 -0.619524447834209 1106 34-35 -0.47976399377833445 1106 36-37 -0.7622274724113183 1106 38-39 -0.6127168199479911 1106 40-41 -0.5279908690921218 1106 42-43 -0.6823574718041598 1106 44-45 -0.5080595262685108 1106 46-47 -0.4457972072639791 1106 48-49 0.11350400324698029 1106 50-51 -0.05556785461486413 1106 52-53 -0.2886645045005949 1106 54-55 -0.5305320644245128 1106 56-57 -0.07466824076686152 1106 58-59 -0.3153710345202825 1106 60-61 0.1393894643336182 1106 62-63 -0.03308571477145961 1106 64-65 -0.4283450812284926 1106 66-67 -0.1023934135055029 1106 68-69 0.343145433966626 1106 70-71 0.28163997830343845 1106 72-73 -0.17853431952703502 1106 74-75 0.10757349939258987 1106 76-77 0.2915775583215634 1106 78-79 0.29481673655527274 1106 80-81 0.23585711040809088 1106 82-83 0.3372279557563971 1106 84-85 0.9465679139141336 1106 86-87 0.8848377651649564 1106 88-89 1.0128457475951613 1106 90-91 0.6873746720139238 1106 92-93 0.8659637352437208 1106 94-95 1.0637711659248446 1106 96-97 1.353330697041823 1106 98-99 1.6991990314935101 1106 100-101 1.5531549994992915 1107 1 0.23692518312819288 1107 2 0.2456392914875991 1107 3 0.044612912989762776 1107 4 0.09123938348066218 1107 5 0.19735904448610597 1107 6 0.30981818361146907 1107 7 0.2599510458887906 1107 8 0.1669562549611996 1107 9 0.03699166130213882 1107 10-11 0.06056456048873571 1107 12-13 0.19942908388731695 1107 14-15 0.2868005501161903 1107 16-17 0.300571437277938 1107 18-19 0.4569441714654232 1107 20-21 0.5234904735382102 1107 22-23 0.7537180478085901 1107 24-25 0.38406993137998313 1107 26-27 0.5528254945330957 1107 28-29 0.5411576459840362 1107 30-31 0.3321617218044466 1107 32-33 0.564550399936806 1107 34-35 0.7457571404942911 1107 36-37 0.40655780693442267 1107 38-39 0.23792098617673219 1107 40-41 0.4102508305389705 1107 42-43 0.40324633280937405 1107 44-45 0.6456361935412573 1107 46-47 0.7359664266753114 1107 48-49 0.7062859046648313 1107 50-51 0.5059015440250789 1107 52-53 0.15611081884711808 1107 54-55 0.3541072528784852 1107 56-57 0.5428480932924344 1107 58-59 0.8317688383629616 1107 60-61 0.6701908332833426 1107 62-63 0.5118940621075083 1107 64-65 0.22248384450153935 1107 66-67 0.5464132001217692 1107 68-69 0.7686754575232371 1107 70-71 1.0478740331503147 1107 72-73 1.4185144381955297 1107 74-75 1.39020828316076 1107 76-77 1.3462911011456917 1107 78-79 1.2200356351729873 1107 80-81 0.7602359716908182 1107 82-83 1.3618957187680891 1107 84-85 1.1780226452355258 1107 86-87 1.1909180353991893 1107 88-89 0.9876224043354647 1107 90-91 1.2393103135979935 1107 92-93 1.286382420518624 1107 94-95 0.5832386978151511 1107 96-97 0.9697358706050885 1107 98-99 1.2618684834691543 1107 100-101 2.8145666177934388 1108 1 0.12438871297582921 1108 2 0.02718886773916296 1108 3 0.02192037374093303 1108 4 0.1674214795303044 1108 5 0.043883381076668115 1108 6 0.44018450603076786 1108 7 0.03913874560629438 1108 8 0.2412858988272646 1108 9 0.11187704082328054 1108 10-11 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-3.4043302884060047 2316 94-95 -3.4813111287379854 2316 96-97 -4.090221556074191 2316 98-99 -3.5067573443454165 2316 100-101 -2.4483630573878905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 8.0 6 14.0 7 47.0 8 88.0 9 146.0 10 252.0 11 318.0 12 479.0 13 701.0 14 783.0 15 978.0 16 1353.0 17 1756.0 18 1643.0 19 1109.0 20 937.0 21 936.0 22 1093.0 23 1227.0 24 1406.0 25 1637.0 26 1861.0 27 2195.0 28 2657.0 29 3221.0 30 3823.0 31 4718.0 32 5697.0 33 7031.0 34 9399.0 35 13631.0 36 18776.0 37 28343.0 38 24670.0 39 2305.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8154334621756 21.21065709552733 15.126311430149089 20.847598012147987 2 14.964782671559684 23.003835005267106 39.70297643227463 22.328405890898576 3 19.38639915726276 28.53877348682535 29.712684434835896 22.362142921076007 4 11.874746111635144 18.043803058365064 38.6915540377717 31.38989679222809 5 11.922782900944208 40.22492958037479 33.744946660146965 14.107340858534032 6 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32.13232028697625 19.794682539409315 28-29 19.7585392554444 27.85026266687781 32.39856514345123 19.992632934226563 30-31 19.92309334141186 28.078848259100393 31.962737794424438 20.03532060506331 32-33 19.628066455064342 28.571822006182828 31.701792193664325 20.09831934508851 34-35 20.437174649516624 27.744305505825324 31.696022805519597 20.12249703913846 36-37 20.599395370937863 27.526116807723827 31.622168814086894 20.25231900725142 38-39 20.331705906251937 28.074838691906706 31.928673245236507 19.66478215660485 40-41 19.87928992453038 28.104858701041152 32.04360160854955 19.972249765878917 42-43 19.509390439138592 28.56552650152347 31.297791396257598 20.62729166308034 44-45 19.931146073604847 28.33993183461287 30.737081282060107 20.991840809722174 46-47 20.32677060992361 28.477790132849535 30.297198764798832 20.898240492428023 48-49 20.367673638006714 28.565969532662017 30.181943368620363 20.884413460710903 50-51 20.248828666935186 28.901718184666127 30.164451375477224 20.685001772921467 52-53 20.265283218925788 29.36395370453247 29.756060616492586 20.61470246004916 54-55 19.967502289298476 28.73293353805055 30.034563243161365 21.26500092948961 56-57 20.479762597382926 29.05565673013698 29.04223053804604 21.42235013443405 58-59 20.595910217570918 28.390939135224457 29.661250344257777 21.351900302946845 60-61 20.49257929363 28.983271996171734 29.798155409662236 20.725993300536025 62-63 20.59447252096559 28.636100744984095 29.931560610859414 20.837866123190903 64-65 20.352041641851162 28.744788744031368 29.873277402341664 21.029892211775802 66-67 20.16951953399342 28.8128124268422 29.572965008193673 21.44470303097071 68-69 19.96970531534013 28.919719085651334 29.606857614982097 21.503717984026437 70-71 20.28738540558591 28.764359803222927 29.374382489732547 21.573872301458614 72-73 20.233955976618173 28.32636789887153 29.299577942867373 22.140098181642927 74-75 19.97335463126803 29.19802259692511 29.16910514248731 21.659517629319545 76-77 20.094037408287694 28.335572319244946 29.736144121800834 21.834246150666527 78-79 19.774817633999366 28.27052220797308 29.981108399178147 21.973551758849403 80-81 19.812584128863904 29.044640367395697 29.225033307284225 21.917742196456174 82-83 20.087304674724678 28.305511279722662 29.32211500394179 22.285069041610868 84-85 19.679016255740457 28.21689467849987 29.289594535978132 22.814494529781534 86-87 19.74146724746361 28.422061093956767 29.537935597706223 22.2985360608734 88-89 19.898156592875107 27.854586407981646 29.31107149766465 22.936185501478594 90-91 19.648275242419842 27.744717908312428 29.638460953644763 22.968545895622967 92-93 19.95311225174796 28.313039407052443 29.23427005552859 22.499578285671006 94-95 19.601558788496362 27.815148615060487 29.20490770512459 23.378384891318568 96-97 19.217369750965638 27.62098856383528 29.21351409037393 23.948127594825152 98-99 19.490708546484807 27.55213748184053 29.022452337838487 23.934701633836177 100-101 19.615457440399346 26.86737240726396 29.58085893794647 23.936311214390223 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 524.0 1 327.0 2 190.5 3 235.5 4 172.5 5 165.0 6 311.5 7 582.5 8 778.0 9 768.0 10 597.5 11 432.0 12 381.0 13 355.5 14 375.0 15 384.0 16 345.0 17 339.5 18 356.0 19 367.5 20 397.5 21 433.0 22 490.5 23 554.5 24 608.5 25 692.0 26 845.0 27 991.0 28 1113.5 29 1296.5 30 1669.0 31 2093.0 32 2575.5 33 3286.0 34 3801.5 35 4256.0 36 5200.0 37 5745.0 38 5515.0 39 5360.5 40 5065.5 41 4725.5 42 4380.5 43 4336.0 44 4613.0 45 4693.0 46 4611.5 47 4913.0 48 6925.0 49 6909.0 50 4897.0 51 4128.5 52 3483.0 53 3176.0 54 3046.0 55 2832.0 56 2593.0 57 2363.5 58 2241.0 59 2087.5 60 1879.0 61 1593.0 62 1289.5 63 990.0 64 707.0 65 527.5 66 362.5 67 275.0 68 237.5 69 166.5 70 115.5 71 93.0 72 68.5 73 58.0 74 56.5 75 42.5 76 30.5 77 22.5 78 17.5 79 13.5 80 7.0 81 3.0 82 5.0 83 6.5 84 3.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2485524060010603 2 0.0 3 0.0 4 0.0 5 0.027540432797901422 6 0.002754043279790142 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0037868095097114453 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010327662299213032 26-27 0.0017212770498688389 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00895064065931796 36-37 0.01927830295853099 38-39 0.015147238038845781 40-41 0.011704683939108102 42-43 0.009294896069291729 44-45 0.004131064919685213 46-47 0.0010327662299213032 48-49 0.002409787869816374 50-51 3.4425540997376773E-4 52-53 0.0 54-55 0.0 56-57 0.0017212770498688389 58-59 6.885108199475355E-4 60-61 0.0037868095097114453 62-63 0.0020655324598426064 64-65 0.0017212770498688389 66-67 0.004819575739632748 68-69 6.885108199475355E-4 70-71 3.4425540997376773E-4 72-73 0.0 74-75 0.0 76-77 0.05921193051548805 78-79 0.1397676964493497 80-81 0.0017212770498688389 82-83 0.0017212770498688389 84-85 0.0 86-87 0.10534215545197292 88-89 0.35045200735329557 90-91 0.38143499425093463 92-93 3.4425540997376773E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.002409787869816374 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 145241.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.22817248573061 #Duplication Level Percentage of deduplicated Percentage of total 1 84.08706388037862 50.64410187206092 2 8.755544377886507 10.546608739956348 3 2.625863093877178 4.7445280602584665 4 1.2117609401435823 2.9192858765775505 5 0.6859024189491975 2.0655324598426064 6 0.4721294983766976 1.7061298118299928 7 0.3372353559833554 1.4217748431916608 8 0.26407243129544106 1.2723679952630456 9 0.19776853079701862 1.0720113466583128 >10 1.2540582559787827 13.46796014899374 >50 0.06973341259316841 2.938564179536081 >100 0.03200877955096255 3.8005797261103957 >500 0.005715853491243312 2.482770016730813 >1k 0.0011431706982486624 0.9177849229900648 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1333 0.9177849229900648 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 964 0.6637244304294242 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 810 0.5576937641575037 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 686 0.47231842248400935 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 608 0.4186145785281016 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 538 0.3704188211317741 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 431 0.2967481633973878 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 326 0.22445452730289656 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 323 0.22238899484305397 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 301 0.20724175680420817 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 300 0.20655324598426064 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 293 0.20173367024462788 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 0.16042302104777578 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 231 0.1590459994078807 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 210 0.14458727218898246 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 209 0.1438987613690349 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 193 0.13288258824987434 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 193 0.13288258824987434 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 179 0.12324343677060885 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 175 0.12048939349081872 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 166 0.11429279611129088 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 161 0.11085024201155322 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 153 0.10534215545197292 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 150 0.10327662299213032 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 149 0.10258811217218279 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 147 0.1012110905322877 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.4425540997376773E-4 0.0 20-21 0.0 0.0 0.0 0.0017212770498688387 0.0 22-23 0.0 0.0 0.0 0.004131064919685213 0.0 24-25 0.0 0.0 0.0 0.00619659737952782 0.0 26-27 0.0 0.0 0.0 0.007917874429396659 0.0 28-29 0.0 0.0 0.0 0.019278302958530995 0.0 30-31 0.0 0.0 0.0 0.04544171411653734 0.0 32-33 0.0 0.0 0.0 0.07539193478425513 0.0 34-35 0.0 0.0 0.0 0.1184238610309761 0.0 36-37 0.0 0.0 0.0 0.18934047548557226 0.0 38-39 0.0 0.0 0.0 0.2850434794582797 0.0 40-41 0.0 0.0 0.0 0.40209031884936075 0.0 42-43 0.0 0.0 0.0 0.518792902830468 0.0 44-45 0.0 0.0 0.0 0.6502984694404472 0.0 46-47 0.0 0.0 0.0 0.8065904255685379 0.0 48-49 0.0 0.0 0.0 0.9838819617050281 0.0 50-51 0.0 0.0 0.0 1.1646160519412563 0.0 52-53 0.0 0.0 0.0 1.347415674637327 0.0 54-55 0.0 0.0 0.0 1.522297422904001 0.0 56-57 0.0 0.0 0.0 1.7584566341460057 0.0 58-59 0.0 0.0 0.0 2.0159596808063838 0.0 60-61 0.0 0.0 0.0 2.316494653713483 0.0 62-63 0.0 0.0 0.0 2.6373406958090344 0.0 64-65 0.0 0.0 0.0 2.9382199241261078 0.0 66-67 0.0 0.0 0.0 3.269737883930846 0.0 68-69 0.0 0.0 0.0 3.669762670320364 0.0 70-71 0.0 0.0 0.0 4.143802369854242 0.0 72-73 0.0 0.0 0.0 4.605104619219091 0.0 74-75 0.0 0.0 0.0 5.075701764653232 0.0 76-77 0.0 0.0 0.0 5.559724871076349 0.0 78-79 0.0 0.0 0.0 6.100894375555112 0.0 80-81 0.0 0.0 0.0 6.718488581048051 0.0 82-83 0.0 0.0 0.0 7.344000660970387 0.0 84-85 0.0 0.0 0.0 7.9715782733525655 0.0 86-87 0.0 0.0 0.0 8.595369076225033 0.0 88-89 0.0 0.0 0.0 9.252896909274929 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATCCC 25 0.00503902 56.923965 4 GTCGGGA 55 8.216157E-7 51.74906 2 GGTCGGG 65 2.5004556E-6 44.03026 1 CGGGAGT 70 4.323654E-6 40.659977 4 TCGGGAG 80 1.0809868E-5 35.57748 3 CCTACAC 75 3.089408E-4 31.624428 3 TACCTGG 75 3.089408E-4 31.624428 2 CGCTGCA 30 0.0039720023 31.624426 24-25 ACTATAC 65 0.005865624 29.191778 3 ACAGCAC 65 0.005865624 29.191778 8 CTACAGT 65 0.005865624 29.191778 4 AGTAGCT 85 6.430038E-4 27.903906 7 ATGGGGT 105 6.9277194E-5 27.106651 7 CTATGAC 70 0.008422528 27.106651 3 ACTGATA 105 6.9277194E-5 27.106651 8 AAGGTAG 35 0.008436175 27.106651 60-61 GCCGCAC 35 0.008436175 27.106651 46-47 GGACAAT 70 0.008422528 27.106651 8 GTGATCT 45 8.584031E-4 26.568691 84-85 GGAGTAA 95 0.0011918932 25.10497 1 >>END_MODULE