FastQCFastQC Report
Thu 26 May 2016
SRR938731_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938731_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4698562
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT141400.30094313962442126No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121520.2586323219742551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92770.19744338799828542No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78890.16790243483006076No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61360.13059314743532172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59920.12752837995965574No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56910.1211221646112151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG1405.977781E-727.0558857
GTACATG169500.022.8494111
TACATGG169300.022.3602392
GAGTACT136300.022.0414512-13
ACATGGG169300.021.464713
GTACTTT142300.020.81284514-15
GTATCAA320800.020.6287881
AGAGTAC221050.020.17716810-11
AGTACTT141450.020.03390512-13
CAACGCA356900.018.359565
TCAACGC358300.018.314274
ATCAACG358750.018.2516773
TATCAAC360450.018.2181742
AACGCAG361900.018.1191526
CATGGGG113050.017.8955044
ACTTTTT176650.017.31595416-17
TACTTTT163050.017.26394814-15
GAGTACA139750.016.9266661
GGTATCA250550.016.7444761
GTGTAGC24500.016.6400661
Produced by FastQC (version 0.11.5)