FastQCFastQC Report
Thu 26 May 2016
SRR938731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4698562
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT156050.3321228920678284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118100.25135349921954847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96690.20578636612648718No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT71160.15145059275582615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65310.1389999748859332No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57910.12325047535820535No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53370.11358794456686962No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG170250.023.172161
TACATGG167350.022.9684732
TAAGGTG23650.022.673585
ACATGGG164550.021.975053
GTAAGGT20800.020.7611244
GAGTACT145750.019.87727412-13
GTACCGT5400.019.3331096
AGAGTAC231850.019.27039510-11
CATGGGG104550.019.2448544
GTACTTT151500.018.96634114-15
AGTACTT155250.018.9360512-13
GTATCAA329200.018.8006381
ATTACGC1559.693424E-418.3692873
TACCGTG4201.8189894E-1218.0777137
AGGTAAG23550.017.3292732
TAGCGCG2504.6086025E-617.0834394
TACTTTT171600.016.82772314-15
CAACGCA370650.016.4773485
TCAACGC373100.016.3691484
ATCAACG373200.016.3266143
Produced by FastQC (version 0.11.5)