##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938731_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4698562 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01914521932455 33.0 31.0 34.0 30.0 34.0 2 32.24676805371516 34.0 31.0 34.0 30.0 34.0 3 32.3426801646972 34.0 31.0 34.0 30.0 34.0 4 35.96040873782234 37.0 35.0 37.0 35.0 37.0 5 35.865885349602706 37.0 35.0 37.0 35.0 37.0 6 35.8933605643599 37.0 35.0 37.0 35.0 37.0 7 35.82842537780708 37.0 35.0 37.0 33.0 37.0 8 35.79084089983276 37.0 35.0 37.0 33.0 37.0 9 37.454591213226514 39.0 37.0 39.0 34.0 39.0 10-11 37.37572878680754 39.0 37.0 39.0 34.0 39.0 12-13 37.307201863038095 39.0 37.0 39.0 34.0 39.0 14-15 38.54206084755293 40.0 38.0 41.0 34.0 41.0 16-17 38.47133782633921 40.0 38.0 41.0 33.5 41.0 18-19 38.48527198321529 40.0 38.0 41.0 33.5 41.0 20-21 38.55386882199277 40.0 38.0 41.0 34.0 41.0 22-23 38.51425425481243 40.0 38.0 41.0 34.0 41.0 24-25 38.43841860552229 40.0 38.0 41.0 34.0 41.0 26-27 38.27789066101501 40.0 38.0 41.0 33.5 41.0 28-29 38.126580430352945 40.0 38.0 41.0 33.0 41.0 30-31 37.918589879201335 40.0 37.0 41.0 33.0 41.0 32-33 37.70972863612313 40.0 37.0 41.0 32.5 41.0 34-35 37.68913999644998 40.0 37.0 41.0 32.0 41.0 36-37 37.615117242254115 40.0 37.0 41.0 32.0 41.0 38-39 37.52061683979056 40.0 37.0 41.0 32.0 41.0 40-41 37.37541283907714 40.0 37.0 41.0 31.5 41.0 42-43 37.208154218248055 40.0 36.0 41.0 31.0 41.0 44-45 36.97739329607654 40.0 36.0 41.0 31.0 41.0 46-47 36.7225168040775 39.5 36.0 41.0 30.0 41.0 48-49 36.46069701751301 39.0 35.0 41.0 30.0 41.0 50-51 36.22624145430027 39.0 35.0 41.0 29.5 41.0 52-53 36.016083431483935 39.0 35.0 40.5 29.0 41.0 54-55 35.768183861360136 38.5 35.0 40.0 28.0 41.0 56-57 35.492678504614815 38.0 34.5 40.0 28.0 41.0 58-59 35.172532681275676 38.0 34.0 40.0 27.5 41.0 60-61 34.80466225623925 37.5 34.0 40.0 27.0 41.0 62-63 34.43431735071283 37.0 34.0 40.0 26.0 41.0 64-65 34.05944339991683 36.5 33.0 39.0 26.0 41.0 66-67 33.65430518954523 36.0 33.0 39.0 26.0 40.5 68-69 33.200599140758385 35.0 32.5 38.5 25.0 40.0 70-71 32.715982358006556 35.0 32.0 38.0 24.0 40.0 72-73 32.35612087272659 35.0 32.0 37.0 23.0 39.5 74-75 31.938284628360762 35.0 31.0 37.0 22.0 39.0 76-77 29.915712828733557 33.0 28.5 35.0 19.5 37.0 78-79 30.98878763332271 34.0 30.5 36.0 20.0 37.5 80-81 31.052890863204528 34.0 31.0 35.0 20.0 37.0 82-83 30.761366456375377 34.0 31.0 35.0 19.5 37.0 84-85 30.386873047540927 34.0 30.5 35.0 18.0 36.0 86-87 29.886656385506885 34.0 30.0 35.0 13.0 36.0 88-89 29.372855035221413 34.0 29.5 35.0 7.0 35.5 90-91 28.964804444423635 34.0 29.0 35.0 2.0 35.0 92-93 28.691514127088244 34.0 29.0 35.0 2.0 35.0 94-95 28.302064759388088 34.0 29.0 35.0 2.0 35.0 96-97 27.775867275987842 33.5 28.0 35.0 2.0 35.0 98-99 27.032337021412083 33.0 27.0 35.0 2.0 35.0 100-101 23.783402560187564 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0826828726304214 1101 2 0.13945121883938327 1101 3 0.15658675924419896 1101 4 0.12565117069421916 1101 5 0.15226369246713034 1101 6 0.19802821441452068 1101 7 0.16094288367081333 1101 8 0.12622229243176264 1101 9 0.2306788547549985 1101 10-11 0.2049550560902773 1101 12-13 0.24367573301214662 1101 14-15 0.34074291420190406 1101 16-17 0.2611741150375124 1101 18-19 0.21006350909099325 1101 20-21 0.2125880512949152 1101 22-23 0.23794428340463725 1101 24-25 0.26171146004461576 1101 26-27 0.27853308579017977 1101 28-29 0.2968367975092363 1101 30-31 0.254520363626483 1101 32-33 0.25687809676789897 1101 34-35 0.32566431636819004 1101 36-37 0.21475558348623025 1101 38-39 0.2447953417080342 1101 40-41 0.36415938742038634 1101 42-43 0.2190001668158459 1101 44-45 0.29879391252924137 1101 46-47 0.2922050802522804 1101 48-49 0.18625909165705679 1101 50-51 0.23527368406921312 1101 52-53 0.2917730206653175 1101 54-55 0.19653972927383023 1101 56-57 0.10547606739133641 1101 58-59 0.10734598381560545 1101 60-61 0.13634439332565762 1101 62-63 0.09098610287040287 1101 64-65 0.053515084829257376 1101 66-67 0.040265482736778324 1101 68-69 0.02922690375670811 1101 70-71 -0.03926493338126846 1101 72-73 0.11540244068513061 1101 74-75 0.21133072638353667 1101 76-77 -0.01034114778348183 1101 78-79 0.08977819944296428 1101 80-81 0.2097670172225108 1101 82-83 0.18457508404624434 1101 84-85 0.11855579895057033 1101 86-87 0.2759414203535009 1101 88-89 0.3684160889068373 1101 90-91 0.43643354732088824 1101 92-93 0.4217107923376382 1101 94-95 0.4904239951655178 1101 96-97 0.4007950171261392 1101 98-99 0.5392580488800647 1101 100-101 1.2397865754171313 1102 1 0.0726600971031175 1102 2 0.0751935363399312 1102 3 0.11169096882156992 1102 4 0.04935672175757588 1102 5 0.07870664611272105 1102 6 0.09968135861239347 1102 7 0.09982246052740607 1102 8 0.06309370294910366 1102 9 0.08876654046233057 1102 10-11 0.16181508248431697 1102 12-13 0.12907206373650837 1102 14-15 0.21052779387865428 1102 16-17 0.2534785467057574 1102 18-19 0.1790430108251897 1102 20-21 0.16993974425733427 1102 22-23 0.15624951100046047 1102 24-25 0.06850200659174988 1102 26-27 0.04237048183433245 1102 28-29 0.007326659916863321 1102 30-31 0.09649270011949795 1102 32-33 0.15761518161981058 1102 34-35 0.054183336285163364 1102 36-37 0.1029188036816393 1102 38-39 0.1345043301417448 1102 40-41 0.15225131487128607 1102 42-43 0.11718980289521852 1102 44-45 -0.08875998477105185 1102 46-47 -0.005866957746057722 1102 48-49 0.004937150018967884 1102 50-51 0.1210033986550556 1102 52-53 0.10220552561365537 1102 54-55 -0.005285574670466531 1102 56-57 -0.01288794468357679 1102 58-59 0.00945002258743699 1102 60-61 -0.1322500751669793 1102 62-63 -0.22663450469347168 1102 64-65 -0.08650788436502665 1102 66-67 -0.050650604275894295 1102 68-69 0.008601516695783573 1102 70-71 -0.025530741447987282 1102 72-73 -0.016269768788561123 1102 74-75 -0.017874719182277232 1102 76-77 -0.06157043069583423 1102 78-79 -0.1259280028073846 1102 80-81 -0.013078943707142798 1102 82-83 -0.019141021139091663 1102 84-85 -0.04712462229402803 1102 86-87 -0.07767656265884781 1102 88-89 0.17565090457883414 1102 90-91 0.25717954667814524 1102 92-93 0.20110714548659914 1102 94-95 0.22697611728230171 1102 96-97 0.34799506593604335 1102 98-99 0.40729686789594766 1102 100-101 0.8871776992665801 1103 1 0.05908774747492629 1103 2 0.10809694681073267 1103 3 0.004845307370651142 1103 4 0.02388646652159565 1103 5 0.04282456698609849 1103 6 0.03694427942794931 1103 7 0.05300018896187453 1103 8 0.09822666967706084 1103 9 0.0016966078853357658 1103 10-11 -0.019431737185158227 1103 12-13 0.08541985890009585 1103 14-15 0.047494582554371334 1103 16-17 -0.001387503555029923 1103 18-19 0.002143630700523147 1103 20-21 0.05248616262691996 1103 22-23 -0.065488337245732 1103 24-25 -0.02519255446481594 1103 26-27 0.050365977329825284 1103 28-29 0.10231467585320786 1103 30-31 0.05212116234294939 1103 32-33 0.08831447847764196 1103 34-35 0.01625890672080743 1103 36-37 -0.054574488712155755 1103 38-39 0.012002624328104616 1103 40-41 0.07056481450698726 1103 42-43 0.10992043468233703 1103 44-45 0.18684666327622068 1103 46-47 0.1114132958708538 1103 48-49 0.17479643484936247 1103 50-51 0.17225326855062661 1103 52-53 0.1882385258796475 1103 54-55 0.24208313550312255 1103 56-57 0.3225423829482921 1103 58-59 0.14886819557428765 1103 60-61 0.24238584926836637 1103 62-63 0.16678365672854056 1103 64-65 0.0727121829423325 1103 66-67 0.08692528997521975 1103 68-69 0.1359010402244465 1103 70-71 0.06804173828463433 1103 72-73 0.08598515783253902 1103 74-75 0.2660112688596783 1103 76-77 0.25337625539976116 1103 78-79 0.21642380824628304 1103 80-81 0.25786483383162917 1103 82-83 0.25929460318869957 1103 84-85 0.21873048184734145 1103 86-87 0.31663577760182804 1103 88-89 0.39152415106023497 1103 90-91 0.42806911403754455 1103 92-93 0.4602486441739906 1103 94-95 0.44464646734995483 1103 96-97 0.562836728844136 1103 98-99 0.7348691047669682 1103 100-101 1.1383898388773055 1104 1 -0.04913818898785394 1104 2 0.020200787493521943 1104 3 0.012736013866614826 1104 4 0.0162352100019163 1104 5 0.09083760634123905 1104 6 0.03661829554933149 1104 7 0.06004570816528343 1104 8 -0.004167118848300788 1104 9 0.050186887638773214 1104 10-11 0.08062765115054304 1104 12-13 0.039918910583359946 1104 14-15 0.05571874092326112 1104 16-17 0.1486136818408923 1104 18-19 0.08387359750943091 1104 20-21 0.029578504969265396 1104 22-23 0.02157757076754052 1104 24-25 0.013792272467384237 1104 26-27 0.1009650764289276 1104 28-29 0.061567046645052415 1104 30-31 0.024222869313078377 1104 32-33 0.057859523522687084 1104 34-35 0.07406995461080612 1104 36-37 0.05149450133050948 1104 38-39 0.06375416534280731 1104 40-41 0.05494698615231641 1104 42-43 0.16186306151969632 1104 44-45 0.12341316398745761 1104 46-47 0.1404885685119055 1104 48-49 0.11087870132636368 1104 50-51 0.03805459619405838 1104 52-53 0.03263136608827466 1104 54-55 -0.024430378010386278 1104 56-57 -0.0307231457382926 1104 58-59 -0.014315303714475647 1104 60-61 -0.030552865762452086 1104 62-63 -0.12880809291583972 1104 64-65 0.11702892073275706 1104 66-67 -0.027080399925203835 1104 68-69 0.050101846293671315 1104 70-71 0.08173886962650556 1104 72-73 -0.030854695178369695 1104 74-75 -0.06732846561163441 1104 76-77 -0.00264887499379185 1104 78-79 0.023422030152403295 1104 80-81 -0.020988414153197965 1104 82-83 0.015413142780925426 1104 84-85 0.030860875398786902 1104 86-87 0.1845638240693468 1104 88-89 0.4235252179165627 1104 90-91 0.4908125943599728 1104 92-93 0.4654604744252957 1104 94-95 0.4708724038127343 1104 96-97 0.4321312997308091 1104 98-99 0.5068398254876847 1104 100-101 0.8086437506838529 1105 1 0.01676618758718007 1105 2 -0.003531300454135078 1105 3 -0.03363806423200799 1105 4 -0.035545984366102346 1105 5 0.00519212510294409 1105 6 -0.008130045606193903 1105 7 -0.0058636622830050555 1105 8 0.017489459886121494 1105 9 0.08876982986243576 1105 10-11 0.05119852484717313 1105 12-13 0.04848902132712141 1105 14-15 0.054567560658306036 1105 16-17 -0.07284852887281801 1105 18-19 -0.042743099598276046 1105 20-21 -0.043384595856110764 1105 22-23 -0.046073782895788895 1105 24-25 -0.0902732952389087 1105 26-27 -0.04215255703002185 1105 28-29 0.05088642333568316 1105 30-31 -0.1494743308811266 1105 32-33 -0.017270779577678752 1105 34-35 0.07293333980952355 1105 36-37 0.030606840359020282 1105 38-39 -0.040288282579886925 1105 40-41 -0.015907729127540904 1105 42-43 0.005507456128164279 1105 44-45 0.045145760570356686 1105 46-47 0.07944443284501546 1105 48-49 0.032805041695823434 1105 50-51 0.01132812635381697 1105 52-53 -0.027931577522565476 1105 54-55 0.05989750676061334 1105 56-57 -0.08487999035022398 1105 58-59 -0.06697192868846713 1105 60-61 -0.09558585769364925 1105 62-63 -0.15070655082627837 1105 64-65 -0.18603648513504112 1105 66-67 -0.0716143975669894 1105 68-69 -0.11275746982312285 1105 70-71 -0.21300547180646134 1105 72-73 -0.2618189519929217 1105 74-75 -0.13801507694495285 1105 76-77 -0.19051228331229098 1105 78-79 -0.13723794719020788 1105 80-81 -0.024176909748877762 1105 82-83 -0.1229499303007131 1105 84-85 -0.1009001816377193 1105 86-87 0.09709375262921327 1105 88-89 0.18707449773530627 1105 90-91 0.2783171142776659 1105 92-93 0.2737156012373063 1105 94-95 0.25908094426079487 1105 96-97 0.17191265593731586 1105 98-99 0.1590475336235393 1105 100-101 0.6124989514791714 1106 1 -0.1189189061211735 1106 2 -0.11416048707584281 1106 3 -0.10179997877342117 1106 4 -0.04803673824691401 1106 5 -0.020836009471103978 1106 6 -0.07310414365287699 1106 7 -0.00924642790141661 1106 8 -0.03279917286764089 1106 9 0.015509157885901459 1106 10-11 -0.11285271939340902 1106 12-13 -0.033324436810275415 1106 14-15 -0.022060356092829636 1106 16-17 -0.1267803248825956 1106 18-19 -0.17337595261872707 1106 20-21 -0.17791819404187947 1106 22-23 -0.1511067667491659 1106 24-25 -0.17273027319966872 1106 26-27 -0.16391094410027307 1106 28-29 -0.12584866964927954 1106 30-31 -0.06074475727304218 1106 32-33 -0.1415319141577669 1106 34-35 -0.16888923579347193 1106 36-37 -0.1099746904045773 1106 38-39 -0.1498966646779678 1106 40-41 -0.10787268901417946 1106 42-43 -0.07815971269960897 1106 44-45 -0.11654471296463242 1106 46-47 -0.15345004061745016 1106 48-49 -0.13094855946042827 1106 50-51 -0.045664961513942615 1106 52-53 -0.1481392079539745 1106 54-55 -0.17298323144185446 1106 56-57 -0.2362168706770973 1106 58-59 -0.1723900721245002 1106 60-61 -0.2033560041609661 1106 62-63 -0.13197435744626773 1106 64-65 -0.07760613050344745 1106 66-67 -0.1567061875625555 1106 68-69 -0.17023719795288628 1106 70-71 -0.1469451207222292 1106 72-73 -0.038727585409645826 1106 74-75 0.02492869328136038 1106 76-77 0.07360944112579659 1106 78-79 -0.019635964258192473 1106 80-81 -0.018991665959919857 1106 82-83 0.1587407941316954 1106 84-85 -0.012480292358564071 1106 86-87 0.028908098980092944 1106 88-89 0.22387613530794326 1106 90-91 0.3285958805363194 1106 92-93 0.3334420745224982 1106 94-95 0.2554191239432697 1106 96-97 0.23396661481467973 1106 98-99 0.251240063876363 1106 100-101 0.7773935177919995 1107 1 -0.052029850273711986 1107 2 -0.07115130179713702 1107 3 -0.11957613445918014 1107 4 -0.07739190004328123 1107 5 -0.039520801554708385 1107 6 -0.10237372548193946 1107 7 0.00905147331609868 1107 8 -0.023006340322339724 1107 9 -0.10016331068241158 1107 10-11 -0.10192006123760677 1107 12-13 -0.15691592937605492 1107 14-15 -0.2018041121345746 1107 16-17 -0.09791316070130307 1107 18-19 -0.06333897513146525 1107 20-21 -0.10742566899992312 1107 22-23 -0.22221248550032868 1107 24-25 -0.16182284606411912 1107 26-27 -0.08844943791476823 1107 28-29 -0.12185865209122682 1107 30-31 -0.12622220032994846 1107 32-33 -0.002890015265244017 1107 34-35 -0.09909571276305229 1107 36-37 -0.032005106639232395 1107 38-39 -0.06344233405761912 1107 40-41 -0.041427356620488354 1107 42-43 0.04670677981137317 1107 44-45 -0.04019662754468811 1107 46-47 -0.11455296363457279 1107 48-49 -0.24082051922524528 1107 50-51 -0.08809266881839051 1107 52-53 -0.08851698277253206 1107 54-55 -0.11642795664705119 1107 56-57 -0.11102184973238138 1107 58-59 -0.04103772323296795 1107 60-61 -0.1496928436242797 1107 62-63 -0.19850846649761422 1107 64-65 -0.21925910620203126 1107 66-67 -0.2084076990673509 1107 68-69 -0.0992611506623291 1107 70-71 -0.10297197346915254 1107 72-73 -0.07759187348295171 1107 74-75 0.024449760566760403 1107 76-77 -0.06144022265544535 1107 78-79 -0.1646196172970953 1107 80-81 -0.05031356865923797 1107 82-83 0.02191660187141764 1107 84-85 -0.03522661653000014 1107 86-87 0.03971074317275125 1107 88-89 0.2654744384681713 1107 90-91 0.3250865082055583 1107 92-93 0.3209729271510575 1107 94-95 0.34238949171975364 1107 96-97 0.3194916447174343 1107 98-99 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-2.73536653567945 2316 98-99 -3.1904365339555874 2316 100-101 -3.759306544671386 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 16.0 4 49.0 5 107.0 6 260.0 7 699.0 8 1600.0 9 3035.0 10 4779.0 11 7316.0 12 9958.0 13 13266.0 14 16243.0 15 21148.0 16 26292.0 17 34397.0 18 32995.0 19 25929.0 20 24849.0 21 26369.0 22 28681.0 23 31776.0 24 37422.0 25 44504.0 26 53974.0 27 66899.0 28 82667.0 29 99564.0 30 120855.0 31 147910.0 32 181289.0 33 229505.0 34 305873.0 35 429410.0 36 661614.0 37 1018253.0 38 825945.0 39 83097.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54692982437398 19.243439396652256 14.754974375497564 23.4546564034762 2 15.862832075005077 20.73855788217757 38.73127565412567 24.66733438869169 3 19.896938680387745 23.963544590876953 29.601354627224246 26.53816210151106 4 13.553700046950535 16.66912131839486 36.15084785515228 33.626330779502325 5 13.993409047278721 37.31284167368655 34.01457722596828 14.67917205306645 6 32.860479440305355 35.77988329195188 16.75099743283158 14.608639834911191 7 28.856041486735727 32.37162774482916 20.41313916896276 18.35919159947235 8 26.52520068906189 34.70974736525771 19.344003548319677 19.421048397360725 9 26.144339480887986 16.943758537186486 20.079569025586977 36.832332956338554 10-11 25.459715121349895 26.144531028855212 26.86627312782081 21.529480721974085 12-13 26.41093168505598 23.357731578299916 27.293925247767298 22.937411488876812 14-15 22.926576414028876 24.98230571698533 25.701080428723618 26.390037440262176 16-17 22.546840337719097 28.022665294018616 26.461941474027345 22.968552894234943 18-19 22.528157856175156 26.938013182987834 28.300219077969814 22.233609882867192 20-21 23.24794266841642 25.836245217153675 28.326166601611302 22.589645512818603 22-23 22.878829735565905 25.758732139748293 28.415842974935735 22.946595149750074 24-25 23.06724908599695 26.037870735769797 28.344289167621923 22.55059101061133 26-27 22.66014082636523 25.992688811361457 28.565715565135815 22.781454797137503 28-29 22.443866868203504 26.75182321740141 28.24409893920736 22.56021097518773 30-31 22.681396989121353 26.09988971093709 28.105428852487208 23.113284447454348 32-33 22.568117649612795 26.14306249443979 28.187698704412117 23.1011211515353 34-35 23.239919198850707 26.239833740599195 27.945252655777463 22.574994404772635 36-37 23.260438370254445 25.769014790866464 28.30187936218167 22.668667476697422 38-39 22.77554865693474 26.301337759859667 28.156079114260507 22.767034468945084 40-41 22.943127205018996 26.28540681433148 28.09352372132444 22.67794225932508 42-43 22.751197188067312 26.293569169370336 27.6654494806802 23.289784161882153 44-45 22.861420233311897 26.15124334504742 27.906919575704837 23.080416845935844 46-47 23.4192185009502 26.597627361852748 27.40723974099725 22.575914396199796 48-49 23.147498717426053 26.608857459397928 27.330344544961065 22.913299278214954 50-51 22.783471035683426 26.748904978993476 27.655260995968963 22.812362989354142 52-53 23.33259622837796 26.704723700570515 27.061162542922705 22.90151752812882 54-55 22.756451867613965 26.571193484304345 27.338172828197223 23.334181819884467 56-57 22.96702693292118 26.71484381817245 27.15619161777582 23.16193763113055 58-59 23.232596474014827 26.40013025308899 27.114298149746357 23.252975123149827 60-61 22.70355788597487 26.62569573555329 27.762773690392184 22.907972688079656 62-63 22.68803731865196 26.554656509800235 27.763962676240094 22.993343495307712 64-65 22.479590564091737 26.93749704696884 27.274004259175467 23.308908129763957 66-67 22.557372813438498 26.72675532826796 27.086646668091944 23.6292251902016 68-69 22.670379606527412 26.474372903995576 27.40798544563898 23.447262043838034 70-71 22.856876205244916 26.52033122665665 27.068519792966843 23.554272775131587 72-73 22.74973704720721 26.391340584629937 27.310089767890688 23.548832600272167 74-75 22.443536980037724 27.194437361899237 26.901017800765427 23.461007857297616 76-77 22.70449853203375 26.490841641795782 27.10189950422984 23.702760321940623 78-79 22.703486032577523 26.669552283675596 27.166661202020588 23.460300481726296 80-81 23.00434846041643 26.528247646964992 26.927976542597793 23.539427350020787 82-83 22.797421849493528 26.526052013360683 27.224627449845297 23.4518986873005 84-85 22.48213389543439 26.84030773670753 27.071750888888985 23.605807478969098 86-87 22.67061151383528 26.69530840850973 26.95954041343948 23.67453966421551 88-89 22.465854165547842 26.751509119524485 26.65458637916266 24.128050335765014 90-91 22.47526851909063 26.53753718085929 26.878160966642046 24.109033333408032 92-93 22.6762358355599 26.57426889333375 26.95163967188259 23.797855599223762 94-95 22.46249675831566 26.24433589819691 27.11845468415273 24.174712659334705 96-97 22.288060353804106 25.933857827306895 27.423037332391488 24.355044486497512 98-99 22.180528539262138 26.361549357690617 26.893340684531953 24.564581418515292 100-101 22.21855677826489 25.608431476482092 27.476374824373508 24.696636920879513 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 773.0 1 555.5 2 442.0 3 559.0 4 523.5 5 523.5 6 895.5 7 1919.5 8 3118.5 9 3627.0 10 3235.5 11 2881.0 12 3065.0 13 3315.0 14 3582.0 15 3931.0 16 4242.0 17 4539.5 18 5035.5 19 5590.0 20 6314.0 21 7584.0 22 9322.5 23 11521.5 24 14087.0 25 17640.5 26 22983.5 27 29237.0 28 34618.5 29 40648.5 30 47896.0 31 57897.5 32 69956.0 33 76394.0 34 80171.5 35 87615.5 36 97981.5 37 106538.0 38 113756.0 39 119482.0 40 123602.0 41 129761.0 42 134131.5 43 138671.0 44 145795.5 45 154548.0 46 161733.5 47 176574.5 48 201810.5 49 199773.5 50 179175.5 51 172982.0 52 173450.0 53 176700.0 54 178670.5 55 169136.5 56 154458.5 57 140762.0 58 131312.0 59 119780.5 60 99067.0 61 81222.5 62 66785.0 63 49939.5 64 35036.0 65 25396.5 66 18709.0 67 14510.0 68 11106.5 69 8904.0 70 6937.0 71 4408.5 72 2921.0 73 2254.5 74 1698.5 75 1166.0 76 779.5 77 477.0 78 271.5 79 169.5 80 121.5 81 81.5 82 58.0 83 40.5 84 22.5 85 12.0 86 8.0 87 5.5 88 6.0 89 4.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38897007211993795 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.234726284339762E-4 16-17 0.002149593854460152 18-19 1.383401985543662E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.1283107469902496E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007693843350369751 36-37 0.01698391976098219 38-39 0.01109914054555415 40-41 0.0070553501262726765 42-43 0.00333080631903974 44-45 0.001862271903616468 46-47 0.001681365490122297 48-49 5.320776867475624E-5 50-51 4.6822836433785487E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.6181282698834243E-4 60-61 5.852854554223186E-4 62-63 0.0 64-65 0.0 66-67 0.0012876280019291008 68-69 8.300411913261972E-4 70-71 0.001862271903616468 72-73 0.0 74-75 0.0 76-77 0.0990302990574563 78-79 0.03626641512871385 80-81 5.320776867475624E-5 82-83 0.0 84-85 0.0 86-87 0.14985435969558344 88-89 0.32883465196372846 90-91 0.28067097975933913 92-93 0.0 94-95 3.192466120485374E-5 96-97 3.192466120485374E-5 98-99 1.8090641349417121E-4 100-101 5.427192404825136E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4698562.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.96032135998773 #Duplication Level Percentage of deduplicated Percentage of total 1 63.508287279149926 23.47286706859802 2 16.03751955087661 11.85503752834971 3 7.0605997805476 7.828861106498944 4 3.8447073886161904 5.6840648247349455 5 2.412606457730263 4.458535499644609 6 1.5336771249136842 3.4011119639563083 7 1.081009074430042 2.796810994879807 8 0.7782215424010124 2.301065463712539 9 0.563339462976966 1.8739086827752418 >10 2.870692633276388 18.76093955963881 >50 0.19181484243744149 4.897694106881738 >100 0.10772230381301894 7.168322351723891 >500 0.005189590495105566 1.312250092999217 >1k 0.0042093336064415975 2.870185622671711 >5k 2.8831052098910685E-4 0.7340739384535696 >10k+ 1.1532420839564274E-4 0.5842711944810304 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15605 0.3321228920678284 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11810 0.25135349921954847 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9669 0.20578636612648718 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7116 0.15145059275582615 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6531 0.1389999748859332 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5791 0.12325047535820535 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5337 0.11358794456686962 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.1283107469902494E-5 2 0.0 0.0 0.0 0.0 2.1283107469902494E-5 3 0.0 0.0 0.0 0.0 2.1283107469902494E-5 4 0.0 0.0 0.0 0.0 2.1283107469902494E-5 5 0.0 0.0 0.0 0.0 2.1283107469902494E-5 6 2.1283107469902494E-5 0.0 0.0 0.0 4.256621493980499E-5 7 4.256621493980499E-5 0.0 0.0 0.0 4.256621493980499E-5 8 4.256621493980499E-5 0.0 0.0 0.0 4.256621493980499E-5 9 4.256621493980499E-5 0.0 0.0 0.0 4.256621493980499E-5 10-11 4.256621493980499E-5 0.0 0.0 2.1283107469902494E-5 6.384932240970748E-5 12-13 5.320776867475623E-5 0.0 0.0 2.1283107469902494E-5 1.383401985543662E-4 14-15 6.384932240970748E-5 0.0 0.0 2.1283107469902494E-5 1.596233060242687E-4 16-17 6.384932240970748E-5 0.0 0.0 4.256621493980499E-5 1.7026485975921995E-4 18-19 7.449087614465872E-5 0.0 0.0 7.449087614465872E-5 1.7026485975921995E-4 20-21 8.513242987960997E-5 0.0 0.0 1.7026485975921995E-4 2.1283107469902493E-4 22-23 8.513242987960997E-5 0.0 0.0 2.979635045786349E-4 2.5539728963882994E-4 24-25 1.2769864481941497E-4 0.0 0.0 5.852854554223186E-4 2.5539728963882994E-4 26-27 1.2769864481941497E-4 0.0 0.0 0.0010109476048203684 2.5539728963882994E-4 28-29 1.383401985543662E-4 0.0 0.0 0.0033520894265096426 2.5539728963882994E-4 30-31 1.4898175228931744E-4 0.0 0.0 0.011280046959048322 2.5539728963882994E-4 32-33 1.4898175228931744E-4 0.0 0.0 0.02656131812243831 2.979635045786349E-4 34-35 1.7026485975921995E-4 0.0 0.0 0.05038775693499416 3.192466120485374E-4 36-37 2.1283107469902493E-4 0.0 0.0 0.08939969292732541 3.192466120485374E-4 38-39 2.1283107469902493E-4 0.0 0.0 0.14782182293220775 3.192466120485374E-4 40-41 2.1283107469902493E-4 0.0 0.0 0.21352277569179678 3.192466120485374E-4 42-43 2.4475573590387867E-4 0.0 0.0 0.2808412446190984 3.192466120485374E-4 44-45 2.979635045786349E-4 0.0 0.0 0.3652819735059365 3.405297195184399E-4 46-47 4.150205956630986E-4 0.0 0.0 0.468622101826048 3.618128269883424E-4 48-49 5.107945792776599E-4 0.0 0.0 0.5783258792796605 3.7245438072329364E-4 50-51 5.107945792776599E-4 0.0 0.0 0.7003525759583464 4.043790419281474E-4 52-53 5.107945792776599E-4 0.0 0.0 0.8198891490630538 4.043790419281474E-4 54-55 5.107945792776599E-4 0.0 0.0 0.9531107602709084 4.469452568679524E-4 56-57 5.533607942174648E-4 0.0 0.0 1.115373171621445 4.6822836433785487E-4 58-59 5.533607942174648E-4 0.0 0.0 1.2885857417652464 4.895114718077573E-4 60-61 5.533607942174648E-4 0.0 0.0 1.479026987405934 4.895114718077573E-4 62-63 5.533607942174648E-4 0.0 0.0 1.670223783361803 5.320776867475623E-4 64-65 5.533607942174648E-4 0.0 0.0 1.8709234868029836 5.640023479524161E-4 66-67 5.533607942174648E-4 0.0 0.0 2.105899634824442 5.746439016873674E-4 68-69 5.533607942174648E-4 0.0 0.0 2.3706614917500293 5.746439016873674E-4 70-71 5.640023479524161E-4 0.0 0.0 2.656185020012506 5.746439016873674E-4 72-73 6.065685628922212E-4 0.0 0.0 2.94885967238487 5.852854554223186E-4 74-75 6.384932240970748E-4 0.0 0.0 3.253271958526885 5.959270091572698E-4 76-77 6.384932240970748E-4 0.0 0.0 3.5817447976636254 5.959270091572698E-4 78-79 6.384932240970748E-4 0.0 0.0 3.9621909852418673 6.06568562892221E-4 80-81 7.555503151815385E-4 0.0 0.0 4.369400680463512 6.172101166271723E-4 82-83 7.874749763863922E-4 0.0 0.0 4.781888586337692 6.384932240970748E-4 84-85 8.406827450611486E-4 0.0 0.0 5.19811167757284 6.597763315669774E-4 86-87 9.151736212058073E-4 0.0 0.0 5.631371896337646 7.023425465067822E-4 88-89 9.577398361456123E-4 0.0 0.0 6.098961767451403 7.236256539766847E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 17025 0.0 23.17216 1 TACATGG 16735 0.0 22.968473 2 TAAGGTG 2365 0.0 22.67358 5 ACATGGG 16455 0.0 21.97505 3 GTAAGGT 2080 0.0 20.761124 4 GAGTACT 14575 0.0 19.877274 12-13 GTACCGT 540 0.0 19.333109 6 AGAGTAC 23185 0.0 19.270395 10-11 CATGGGG 10455 0.0 19.244854 4 GTACTTT 15150 0.0 18.966341 14-15 AGTACTT 15525 0.0 18.93605 12-13 GTATCAA 32920 0.0 18.800638 1 ATTACGC 155 9.693424E-4 18.369287 3 TACCGTG 420 1.8189894E-12 18.077713 7 AGGTAAG 2355 0.0 17.329273 2 TAGCGCG 250 4.6086025E-6 17.083439 4 TACTTTT 17160 0.0 16.827723 14-15 CAACGCA 37065 0.0 16.477348 5 TCAACGC 37310 0.0 16.369148 4 ATCAACG 37320 0.0 16.326614 3 >>END_MODULE