FastQCFastQC Report
Thu 26 May 2016
SRR938730_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938730_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4713128
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT142950.30330175628584666No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122300.25948796637816757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93180.1977030965422539No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78150.16581344703559928No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61730.13097458842620016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59570.12639164478452528No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56800.12051444391071069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG167550.024.1110081
TACATGG170050.023.1553612
TAAGGTG21150.022.6548445
GTAAGGT18600.022.4450114
ACATGGG167600.022.3616163
GAGTACT135350.021.16580812-13
CATGGGG108400.020.0440884
GTACTTT142400.019.8846814-15
AGAGTAC221650.019.58370410-11
AGGTAAG20550.019.3917752
GTATCAA329250.019.328391
AGTACTT142500.019.10444312-13
GGTAAGG21900.018.8462643
GTGTACG5050.017.8814641
CAACGCA362350.017.282075
TCAACGC366200.017.1651524
ATCAACG367650.017.0974523
TATCAAC370850.017.0266762
AACGCAG368950.017.0107696
ACTTTTT173500.016.8265916-17
Produced by FastQC (version 0.11.5)