##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938730_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4713128 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.440439343043515 33.0 31.0 34.0 30.0 34.0 2 31.50902691376088 33.0 31.0 34.0 28.0 34.0 3 31.53106493182447 33.0 31.0 34.0 28.0 34.0 4 35.06825106383701 37.0 35.0 37.0 32.0 37.0 5 35.095155913440074 37.0 35.0 37.0 32.0 37.0 6 35.14471450807192 37.0 35.0 37.0 32.0 37.0 7 35.045441795767054 37.0 35.0 37.0 32.0 37.0 8 35.00847823356378 37.0 35.0 37.0 32.0 37.0 9 36.56505785542001 39.0 37.0 39.0 32.0 39.0 10-11 36.44400438519811 39.0 37.0 39.0 32.0 39.0 12-13 36.327024748744364 39.0 37.0 39.0 32.0 39.0 14-15 37.47687576064134 40.0 37.0 41.0 32.0 41.0 16-17 37.359618919749266 40.0 37.0 41.0 32.0 41.0 18-19 37.28485042205516 40.0 37.0 41.0 31.0 41.0 20-21 37.15655759826595 40.0 37.0 41.0 31.0 41.0 22-23 37.180334482746915 40.0 37.0 41.0 31.0 41.0 24-25 37.106087825325346 40.0 37.0 41.0 31.0 41.0 26-27 36.96472289740487 40.0 37.0 41.0 30.0 41.0 28-29 36.79654042920116 40.0 36.0 41.0 30.0 41.0 30-31 36.57950558525039 39.0 36.0 41.0 30.0 41.0 32-33 36.3912759212141 39.0 35.5 41.0 30.0 41.0 34-35 36.1020157950304 39.0 35.0 41.0 28.5 41.0 36-37 35.88803645901406 39.0 35.0 41.0 27.5 41.0 38-39 35.71279784890204 39.0 35.0 40.5 27.0 41.0 40-41 35.61201637214181 39.0 35.0 40.5 27.0 41.0 42-43 35.61769550922445 39.0 35.0 41.0 26.5 41.0 44-45 35.457882323586375 39.0 35.0 41.0 26.0 41.0 46-47 35.18326491451113 39.0 35.0 41.0 24.5 41.0 48-49 34.898957550060175 39.0 34.0 41.0 24.0 41.0 50-51 33.75690443798683 38.0 32.5 39.5 23.0 40.5 52-53 33.822472676320274 37.5 33.0 39.5 23.0 40.5 54-55 34.31633036488718 38.0 34.0 40.0 23.0 41.0 56-57 34.18645631945493 38.0 34.0 40.0 22.5 41.0 58-59 33.95818360969615 38.0 33.5 40.0 22.0 41.0 60-61 33.61835367085298 37.0 33.0 40.0 20.5 41.0 62-63 33.2150051940028 37.0 33.0 40.0 19.5 41.0 64-65 32.81061961398035 36.0 32.0 39.0 18.0 41.0 66-67 32.40420565704984 36.0 32.0 39.0 15.5 41.0 68-69 31.97376731546438 35.0 31.0 39.0 13.0 40.0 70-71 31.52945199027058 35.0 31.0 38.0 9.0 40.0 72-73 31.110811439876024 35.0 31.0 37.0 7.5 39.5 74-75 30.653367360275382 35.0 30.0 37.0 6.0 39.0 76-77 30.20863012844124 34.5 30.0 36.0 2.0 39.0 78-79 29.776659152902276 34.0 29.5 36.0 2.0 38.0 80-81 29.31568461539767 34.0 29.0 35.0 2.0 37.0 82-83 28.906913943351423 34.0 29.0 35.0 2.0 37.0 84-85 28.559401208708948 34.0 29.0 35.0 2.0 36.0 86-87 28.158821063208975 34.0 27.5 35.0 2.0 36.0 88-89 27.698470527428917 34.0 26.5 35.0 2.0 35.5 90-91 27.24354377814479 33.0 26.0 35.0 2.0 35.0 92-93 26.780485698669757 33.0 25.0 35.0 2.0 35.0 94-95 26.253370267049824 33.0 24.5 35.0 2.0 35.0 96-97 25.631051713426835 33.0 21.5 35.0 2.0 35.0 98-99 24.838295925763106 32.0 10.0 35.0 2.0 35.0 100-101 22.333428563790335 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13890539086466447 1101 2 0.2130332848249914 1101 3 0.3415228435718376 1101 4 0.08633354360627266 1101 5 0.0781086729814291 1101 6 0.2403209514173099 1101 7 0.30006598653110217 1101 8 0.36195360865435333 1101 9 0.44530409750474576 1101 10-11 0.2842447469270226 1101 12-13 0.2946008654423906 1101 14-15 0.29795533739627444 1101 16-17 0.44953496200042053 1101 18-19 0.5003393150466593 1101 20-21 0.562848247896099 1101 22-23 0.540526033619436 1101 24-25 0.5278768635237228 1101 26-27 0.5152731151279113 1101 28-29 0.6167710227836878 1101 30-31 0.5492258008574993 1101 32-33 0.38708165868449385 1101 34-35 0.4858353849945516 1101 36-37 0.4498386853012377 1101 38-39 0.48208776881618576 1101 40-41 0.6444971384956517 1101 42-43 0.6269201389392904 1101 44-45 0.6219438594253077 1101 46-47 0.47575871537506487 1101 48-49 0.6194333929590883 1101 50-51 0.5879669757677632 1101 52-53 0.5287621214360314 1101 54-55 0.6519621511883926 1101 56-57 0.5988753971755472 1101 58-59 0.5003401202502573 1101 60-61 0.42380039144869386 1101 62-63 0.36449710074365527 1101 64-65 0.28572226112594734 1101 66-67 0.29466389697754636 1101 68-69 0.4087427792149114 1101 70-71 0.3335534586572315 1101 72-73 0.29755324386254856 1101 74-75 0.23080310539847915 1101 76-77 0.1404159099838047 1101 78-79 0.07096167006955412 1101 80-81 0.06476858021439469 1101 82-83 0.1441777604010852 1101 84-85 0.2090422288224012 1101 86-87 0.006589754443456997 1101 88-89 -0.045334606032838565 1101 90-91 -0.12278702752937676 1101 92-93 -0.1544370831968891 1101 94-95 -0.2618597746970508 1101 96-97 -0.19264136457139713 1101 98-99 -0.29941933244312935 1101 100-101 0.11784714429768428 1102 1 0.18030811260967994 1102 2 0.11967262645370624 1102 3 0.19128804261238486 1102 4 0.29163112552558346 1102 5 0.2557711190502232 1102 6 0.31154018892114976 1102 7 0.29100422831221806 1102 8 0.26760435567373264 1102 9 0.5244068831561748 1102 10-11 0.5059811689680487 1102 12-13 0.472957995279387 1102 14-15 0.5450717454771663 1102 16-17 0.7095424943680797 1102 18-19 0.6933094856515325 1102 20-21 0.6476911065455866 1102 22-23 0.569182833345856 1102 24-25 0.5013087901749387 1102 26-27 0.5440738796031823 1102 28-29 0.6411754540012424 1102 30-31 0.6529114725974381 1102 32-33 0.6193152926173866 1102 34-35 0.7000648543540606 1102 36-37 0.7498024626520774 1102 38-39 0.7582805185937644 1102 40-41 0.847545826952036 1102 42-43 0.8833402861023316 1102 44-45 0.9258029809580037 1102 46-47 1.0362129555540065 1102 48-49 0.9700829828978925 1102 50-51 0.9019828309571025 1102 52-53 0.7629011684663922 1102 54-55 0.7478822745406504 1102 56-57 0.8536589937743102 1102 58-59 0.8528370973801174 1102 60-61 0.8024679690518042 1102 62-63 0.7005361020362244 1102 64-65 0.7308175159680701 1102 66-67 0.7523808635646603 1102 68-69 0.7512468106633037 1102 70-71 0.7810929287633677 1102 72-73 0.6531014853223667 1102 74-75 0.5478701561667023 1102 76-77 0.5196151396417683 1102 78-79 0.45396320078929975 1102 80-81 0.47946822900182795 1102 82-83 0.5535109982773712 1102 84-85 0.5476712664068266 1102 86-87 0.45609484067082207 1102 88-89 0.5569223496393576 1102 90-91 0.5422627131851101 1102 92-93 0.38152561815380537 1102 94-95 0.30018282154333775 1102 96-97 0.1531274491256127 1102 98-99 0.10281698415666796 1102 100-101 0.48752955237830164 1103 1 0.18339799236282772 1103 2 0.35162255905338213 1103 3 0.3832476253301387 1103 4 0.35770756856754105 1103 5 0.3268282000826872 1103 6 0.3441376408497021 1103 7 0.3683282703369599 1103 8 0.37741366925067865 1103 9 0.4888904502136029 1103 10-11 0.41646583356916267 1103 12-13 0.485919568446306 1103 14-15 0.5063113363894303 1103 16-17 0.5429457176782364 1103 18-19 0.6245014257363408 1103 20-21 0.6016248437492422 1103 22-23 0.593915551356865 1103 24-25 0.5692714988480745 1103 26-27 0.544476551895194 1103 28-29 0.5840693977210165 1103 30-31 0.7027853988364825 1103 32-33 0.6533796029270817 1103 34-35 0.6187771206739612 1103 36-37 0.687152844826862 1103 38-39 0.6760039847562425 1103 40-41 0.7484967769023854 1103 42-43 0.7289933189821483 1103 44-45 0.747380609037144 1103 46-47 0.7736698798843733 1103 48-49 0.71449313627771 1103 50-51 0.7100668040562752 1103 52-53 0.7481275025963399 1103 54-55 0.7349777574722296 1103 56-57 0.7293066198025855 1103 58-59 0.7116833871276071 1103 60-61 0.6716875516931822 1103 62-63 0.6342059615232216 1103 64-65 0.51254804091338 1103 66-67 0.4369617225015432 1103 68-69 0.3930395267559774 1103 70-71 0.4506995045011841 1103 72-73 0.4962382768692599 1103 74-75 0.2868778382986399 1103 76-77 0.33051294430729783 1103 78-79 0.44000833485877777 1103 80-81 0.4459565092523974 1103 82-83 0.5151057297082495 1103 84-85 0.6454322638013394 1103 86-87 0.5338503750562218 1103 88-89 0.6125506080423868 1103 90-91 0.5115298500629137 1103 92-93 0.45119461865450816 1103 94-95 0.4499997141469123 1103 96-97 0.4928282954652268 1103 98-99 0.5352261509601703 1103 100-101 0.8760396446811392 1104 1 -0.021759152024046102 1104 2 0.08461231628507448 1104 3 0.12141634224277809 1104 4 0.24040043605270967 1104 5 0.2107947792295235 1104 6 0.19244131329040215 1104 7 0.2441019206985402 1104 8 0.22987692411472693 1104 9 0.2929017152412712 1104 10-11 0.27465547010729807 1104 12-13 0.2729601974362197 1104 14-15 0.2935778556550659 1104 16-17 0.3124790189241722 1104 18-19 0.4058082460973722 1104 20-21 0.41367814844482353 1104 22-23 0.2739194843174104 1104 24-25 0.20578373880211842 1104 26-27 0.19705118891109663 1104 28-29 0.20541546344136208 1104 30-31 0.297157252495758 1104 32-33 0.2600388101229427 1104 34-35 0.39008504288469936 1104 36-37 0.4158773351770222 1104 38-39 0.37170621663374703 1104 40-41 0.45886250010931207 1104 42-43 0.4015068465706264 1104 44-45 0.4609461747482939 1104 46-47 0.6254760820345524 1104 48-49 0.5139501039474013 1104 50-51 0.5251378910986304 1104 52-53 0.5426182097022973 1104 54-55 0.4782616065946428 1104 56-57 0.41432545433823265 1104 58-59 0.4862303927678795 1104 60-61 0.5172551561588534 1104 62-63 0.4519030202495955 1104 64-65 0.5676235220525712 1104 66-67 0.559009203134373 1104 68-69 0.5189101155050935 1104 70-71 0.5575676640818337 1104 72-73 0.40587132544354176 1104 74-75 0.4246484482644597 1104 76-77 0.4062638260641904 1104 78-79 0.21848080757136756 1104 80-81 0.18439995698226497 1104 82-83 0.21068094957382755 1104 84-85 0.28940673103926784 1104 86-87 0.31470616864629264 1104 88-89 0.28769775596980907 1104 90-91 0.34373585294900977 1104 92-93 0.33941156643325954 1104 94-95 0.3617214662786168 1104 96-97 0.23133377489511275 1104 98-99 0.4100632955776149 1104 100-101 0.7114847309967622 1105 1 -0.05884680560648192 1105 2 0.008885899559334831 1105 3 0.07799212576753334 1105 4 0.13231067836246524 1105 5 0.10693864740563441 1105 6 0.1096633775778244 1105 7 0.1343804431017972 1105 8 0.09395040640707464 1105 9 0.06800249335008601 1105 10-11 0.1422583326374749 1105 12-13 0.19262658941774902 1105 14-15 0.20815905303827975 1105 16-17 0.23522005166281446 1105 18-19 0.2359758419005331 1105 20-21 0.23591898076333706 1105 22-23 0.19549171040657853 1105 24-25 0.22504917778638145 1105 26-27 0.24631434357420545 1105 28-29 0.33389137807609615 1105 30-31 0.35575809018425275 1105 32-33 0.30046533208008697 1105 34-35 0.35519615680953365 1105 36-37 0.4417551423813606 1105 38-39 0.42662176526368256 1105 40-41 0.44977733690523536 1105 42-43 0.3890916608823787 1105 44-45 0.44333725885380915 1105 46-47 0.5441364924595931 1105 48-49 0.534197111564751 1105 50-51 0.5556850493216032 1105 52-53 0.7591521677752056 1105 54-55 0.7170498522793665 1105 56-57 0.5629374343798261 1105 58-59 0.5656391065597859 1105 60-61 0.6584626488362701 1105 62-63 0.663279303792649 1105 64-65 0.5861334829589353 1105 66-67 0.5737303043471442 1105 68-69 0.5845489865335693 1105 70-71 0.5272155490727677 1105 72-73 0.6287477870318234 1105 74-75 0.5535368675888108 1105 76-77 0.5343258484186215 1105 78-79 0.4893313954501757 1105 80-81 0.6254423567232017 1105 82-83 0.6393041309411203 1105 84-85 0.6907547797138669 1105 86-87 0.660025452392798 1105 88-89 0.7265695736390079 1105 90-91 0.8235490971203916 1105 92-93 0.7671179839880864 1105 94-95 0.8845175213236445 1105 96-97 0.6907227250596186 1105 98-99 0.5090977883065513 1105 100-101 1.0516796640706652 1106 1 0.0286952977971211 1106 2 0.03293758453670392 1106 3 0.0414100304397067 1106 4 0.03158705503019377 1106 5 0.09888019896443012 1106 6 0.2041896827412728 1106 7 0.11183808733014189 1106 8 0.06336945514775749 1106 9 0.009226141919157271 1106 10-11 0.05481221321691265 1106 12-13 0.14638224666828137 1106 14-15 0.23337903385830572 1106 16-17 0.2941632183771503 1106 18-19 0.152191582937256 1106 20-21 0.3068761286678452 1106 22-23 0.29518079296457955 1106 24-25 0.15853463297422365 1106 26-27 0.014260319986199477 1106 28-29 -0.03772991842271978 1106 30-31 -0.03506765808702994 1106 32-33 0.07325046591927986 1106 34-35 0.1103617102526755 1106 36-37 0.276458021059625 1106 38-39 0.23345984592051394 1106 40-41 0.4607055466062633 1106 42-43 0.4368582036082884 1106 44-45 0.4124305004373312 1106 46-47 0.4136929976667787 1106 48-49 0.40451711425667725 1106 50-51 0.3350947604443064 1106 52-53 0.2055311068199046 1106 54-55 0.2292599234471524 1106 56-57 0.41647883115287954 1106 58-59 0.5087088274407137 1106 60-61 0.5227548405686946 1106 62-63 0.48056281406402945 1106 64-65 0.5049315380424773 1106 66-67 0.5833191126652011 1106 68-69 0.5661581693658064 1106 70-71 0.4902754275996166 1106 72-73 0.40202428147131286 1106 74-75 0.35825985158867724 1106 76-77 0.34513822118306337 1106 78-79 0.4494559811459524 1106 80-81 0.5284658101276491 1106 82-83 0.4388862935670943 1106 84-85 0.5985483258215076 1106 86-87 0.5763293540029082 1106 88-89 0.8058294622605722 1106 90-91 0.8799519270074967 1106 92-93 0.8938540238285917 1106 94-95 0.9587265157210751 1106 96-97 1.0647432005210327 1106 98-99 1.0893753090427936 1106 100-101 1.4022495111356257 1107 1 0.050018394443487324 1107 2 0.12253449937851357 1107 3 0.19462622044416023 1107 4 0.11615631083775924 1107 5 0.10058706731242495 1107 6 -0.0187523727266381 1107 7 0.08215759184007965 1107 8 0.14448842853764177 1107 9 0.20017832862700402 1107 10-11 0.19716398035656368 1107 12-13 0.19196814368260107 1107 14-15 0.3121620155396556 1107 16-17 0.3652356771383438 1107 18-19 0.2547050794637542 1107 20-21 0.21703679664704367 1107 22-23 0.2454114097880975 1107 24-25 0.1681431816761858 1107 26-27 0.16599871838452174 1107 28-29 0.17410968831534746 1107 30-31 0.15569199760065544 1107 32-33 0.23652490847215546 1107 34-35 0.28194751569457566 1107 36-37 0.2745010351243167 1107 38-39 0.09590730624260146 1107 40-41 0.09229620122421522 1107 42-43 0.21877441900327455 1107 44-45 0.483873244779609 1107 46-47 0.34488420921157115 1107 48-49 0.2310492457713309 1107 50-51 0.4269032949941689 1107 52-53 0.2806551458297548 1107 54-55 0.33679922674750884 1107 56-57 0.48217053594003545 1107 58-59 0.5754061847438265 1107 60-61 0.5487599103510448 1107 62-63 0.4252581484490605 1107 64-65 0.5140077179739606 1107 66-67 0.39740646859472406 1107 68-69 0.5441852415040671 1107 70-71 0.6010974565947684 1107 72-73 0.547119600039288 1107 74-75 0.582287435700028 1107 76-77 0.5269218438713814 1107 78-79 0.6077777291802668 1107 80-81 0.6450139668163892 1107 82-83 0.4320419209006303 1107 84-85 0.6185375452498505 1107 86-87 0.6566425108789566 1107 88-89 0.7303599662835154 1107 90-91 0.7602569256130991 1107 92-93 0.8484726663090392 1107 94-95 0.8736518425721869 1107 96-97 0.9793777016802565 1107 98-99 1.1465342746074008 1107 100-101 1.3883417620274159 1108 1 0.13128901613551847 1108 2 0.1993127835524824 1108 3 0.1480911709810293 1108 4 0.18277590290977486 1108 5 0.3062468583941609 1108 6 0.22354107223324604 1108 7 0.117091199213192 1108 8 0.21483113556460154 1108 9 0.2631737103082159 1108 10-11 0.3125414432067828 1108 12-13 0.3891814940268006 1108 14-15 0.48061542841612237 1108 16-17 0.5490991714817994 1108 18-19 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-2.5791081506476914 2316 48-49 -2.2712046563457164 2316 50-51 -2.304579410059546 2316 52-53 -2.563823013658368 2316 54-55 -2.47361076800491 2316 56-57 -2.1337274296244075 2316 58-59 -1.9032184838737862 2316 60-61 -1.7902203550805993 2316 62-63 -2.022965884323405 2316 64-65 -2.1122614394270656 2316 66-67 -1.8730041018573758 2316 68-69 -2.122553292934988 2316 70-71 -2.0863387501048933 2316 72-73 -2.023284399103982 2316 74-75 -1.9467532232791527 2316 76-77 -1.9906541347565287 2316 78-79 -1.8042120877529157 2316 80-81 -1.7267943607930896 2316 82-83 -1.8225875661717872 2316 84-85 -1.8628590226331916 2316 86-87 -2.027039698353409 2316 88-89 -2.179287548331967 2316 90-91 -2.4946891773451085 2316 92-93 -2.1965097038351793 2316 94-95 -2.1844331492591245 2316 96-97 -2.3266102377902236 2316 98-99 -2.691964959655216 2316 100-101 -2.561724585466802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26688.0 3 7204.0 4 6663.0 5 7555.0 6 8588.0 7 10144.0 8 12398.0 9 14566.0 10 17131.0 11 20279.0 12 23737.0 13 27179.0 14 28961.0 15 31005.0 16 35412.0 17 41274.0 18 38363.0 19 32910.0 20 34038.0 21 36638.0 22 40418.0 23 45057.0 24 51154.0 25 57523.0 26 65657.0 27 76629.0 28 88492.0 29 105299.0 30 125167.0 31 149303.0 32 182849.0 33 231258.0 34 305345.0 35 425301.0 36 623896.0 37 870465.0 38 704516.0 39 104046.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92962983687427 18.382967913351944 14.143392600652282 23.5440096491215 2 15.402427249567019 20.017423420792614 39.84501274239075 24.735136587249617 3 19.77167687813838 23.80899941749967 29.35119242494462 27.06813127941733 4 13.03202285470422 15.977124892831382 36.69642663588088 34.29442561658352 5 14.021965522734481 37.06253935973345 34.065616676765586 14.849878440766487 6 32.82470028385657 35.305809110282276 17.15827087298226 14.711219732878892 7 28.8935510601335 32.29617038364224 20.57151158141189 18.238766974812364 8 26.47590893843971 34.280067324755585 19.66454183453365 19.579481902271052 9 26.402264013502823 16.99406223444686 20.20319257693245 36.40048117511787 10-11 25.41859370375536 25.96082537004215 26.999394886257328 21.62118603994516 12-13 26.448700680621428 23.26077616933003 27.35858093424352 22.931942215805016 14-15 23.07843625490069 24.79753478373936 25.807318260069646 26.3167107012903 16-17 22.613447223511958 27.848059516144062 26.504373445844642 23.034119814499334 18-19 22.60996466847425 26.819994670390447 28.319941608236267 22.250099052899035 20-21 23.34215524415773 25.787213895142035 28.26378012979126 22.606850730908974 22-23 22.98056083381785 25.646633068948137 28.39681835908607 22.975987738147946 24-25 23.073422933408196 25.89663345742933 28.381265013542095 22.648678595620378 26-27 22.665624252814315 25.90830633085441 28.58900896377974 22.83706045255154 28-29 22.485644516128005 26.66581880934225 28.28146592757631 22.567070746953434 30-31 22.691353206499397 25.99522946978125 28.128508525157535 23.184908798561818 32-33 22.544720537167287 26.0738615648388 28.228196180431915 23.153221717561998 34-35 23.293863613859013 26.172757482157706 27.91006397991484 22.623314924068435 36-37 23.28140444459593 25.70174298715021 28.296880858243295 22.71997171001057 38-39 22.796749809884474 26.22141704514947 28.153572239054526 22.82826090591153 40-41 22.9691217166515 26.164402577157386 28.13840362208902 22.728072084102095 42-43 22.71193357003555 26.239156561458344 27.680028607020006 23.368881261486095 44-45 22.838586085092533 26.105946918126254 27.92378852448681 23.131678472294404 46-47 23.441445776408713 26.52822744307911 27.432418140857056 22.597908639655117 48-49 23.156108726222172 26.51900985333918 27.355817724676406 22.96906369576224 50-51 22.796875996914896 26.704638594750357 27.678453025609752 22.820032382724996 52-53 23.323512247101526 26.616205292905438 27.158837971036938 22.9014444889561 54-55 22.754159884280412 26.479003990136015 27.432001374388467 23.334834751195107 56-57 22.93911733602446 26.67572653302405 27.237367966131348 23.14778816482014 58-59 23.247044184752415 26.26042728708863 27.206360558625903 23.286167969533057 60-61 22.744232174626262 26.52920311959405 27.833968447117265 22.89259625866242 62-63 22.763791963212203 26.423040509815156 27.876703794540404 22.936463732432237 64-65 22.540525562720543 26.831068942914094 27.39711594503504 23.231289549330324 66-67 22.64365085283871 26.62770207643054 27.203658523592892 23.524988547137852 68-69 22.75695758772657 26.35794579680856 27.547159197210103 23.337937418254764 70-71 22.981055963785714 26.387865043225965 27.16615939915889 23.464919593829432 72-73 22.833344625820253 26.330585210762546 27.44862664183172 23.38744352158548 74-75 22.614355927207384 27.11445984574037 27.02915183834601 23.242032388706235 76-77 22.90638854133637 26.491625170724415 27.101029975601442 23.500956312337777 78-79 22.942774225603387 26.675950616316396 27.19358866537509 23.18768649270513 80-81 23.26258435389023 26.5565380727883 26.908474318515562 23.27240325480591 82-83 23.103148112784236 26.559265381772086 27.223649342448397 23.11393716299528 84-85 22.861921074751443 26.885571639090532 27.067635058593876 23.184872227564153 86-87 23.02782244051791 26.748433117947513 27.013218052875406 23.21052638865917 88-89 22.926012771715833 26.760158339027225 26.666577907626337 23.647250981630606 90-91 22.93767679186734 26.604155818034453 26.862829113055604 23.595338277042604 92-93 23.30289311185809 26.60883944316351 26.87523443792904 23.213033007049358 94-95 23.06252933882774 26.31290388498817 27.083251207699615 23.54131556848447 96-97 23.02052628141565 26.023296267715413 27.344918216737817 23.611259234131126 98-99 22.93634584426695 26.39880505295546 26.854559730838606 23.810289371938985 100-101 23.262500816134857 25.6042353591265 27.279109173760517 23.85415465097813 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3880.0 1 2585.5 2 1212.0 3 1172.0 4 1157.5 5 1195.0 6 1627.0 7 2723.0 8 4022.0 9 4586.0 10 4166.5 11 3715.5 12 3811.0 13 4024.5 14 4333.5 15 4629.5 16 4877.0 17 5183.5 18 5647.0 19 6178.0 20 6825.5 21 8094.5 22 9819.0 23 11943.0 24 14480.5 25 17984.0 26 23279.5 27 29649.0 28 34934.0 29 40755.5 30 47837.0 31 57540.0 32 69524.0 33 76354.0 34 80565.0 35 87368.5 36 97823.0 37 107112.0 38 113442.0 39 118857.5 40 123727.0 41 130245.5 42 133923.0 43 138019.5 44 145798.5 45 154788.0 46 161380.0 47 175043.0 48 199526.5 49 198876.0 50 177988.0 51 170699.0 52 171602.0 53 174307.0 54 176010.0 55 167542.0 56 153518.5 57 139824.5 58 130896.0 59 119421.0 60 99309.5 61 81937.0 62 67955.0 63 51690.0 64 36499.0 65 26699.0 66 19828.5 67 15483.5 68 12030.0 69 9582.0 70 7626.5 71 5160.0 72 3512.0 73 2641.5 74 1954.0 75 1415.0 76 1028.5 77 698.0 78 480.0 79 333.5 80 250.5 81 199.0 82 161.5 83 128.5 84 89.5 85 64.0 86 58.0 87 49.5 88 39.0 89 29.5 90 18.5 91 15.5 92 13.5 93 10.5 94 10.0 95 7.5 96 5.5 97 4.0 98 2.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27605870241589026 2 0.16903848145011127 3 0.19664222995853287 4 0.16816857085146 5 0.2398619345793282 6 0.19825474716578884 7 0.24306575166216574 8 0.24902782186267802 9 0.15168270414043497 10-11 0.20962723694327845 12-13 0.2152816558345116 14-15 0.17626298288525158 16-17 0.2891922307223568 18-19 0.23540629492769982 20-21 0.20153282490948687 22-23 0.24861408389502682 24-25 0.2360003802145836 26-27 0.3466275475650142 28-29 0.31848275709889484 30-31 0.2940722170074736 32-33 0.19505093008295127 34-35 0.36330437026110896 36-37 0.4007529606664619 38-39 0.3809890162117388 40-41 0.3430842531753859 42-43 0.37718050517618024 44-45 0.3859856978210649 46-47 0.3910884660887631 48-49 0.4103113685857885 50-51 0.4022487825495085 52-53 0.4026306945196481 54-55 0.39626549501732183 56-57 0.3754300753130405 58-59 0.35001171196708425 60-61 0.31589424263461546 62-63 0.38388518198529725 64-65 0.4180132599836033 66-67 0.4238798521915806 68-69 0.4284627958332556 70-71 0.4280278405339299 72-73 0.4188725619164173 74-75 0.41474579090574243 76-77 0.39874792282322913 78-79 0.37276730018790066 80-81 0.3840655293045298 82-83 0.3936557632213681 84-85 0.3948545424609729 86-87 0.3898578608516467 88-89 0.3982599241947174 90-91 0.4191696045598592 92-93 0.4184376066170917 94-95 0.4267866266309763 96-97 0.4274125379153717 98-99 0.4250468054336738 100-101 0.39770827357118244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4713128.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.884293873874235 #Duplication Level Percentage of deduplicated Percentage of total 1 67.12584877098946 26.772670789153835 2 14.64109521122463 11.678994880797118 3 6.364404088302226 7.615192889697979 4 3.4613728641016355 5.522176500755335 5 2.1282479800650895 4.244183393669764 6 1.3484197436427103 3.226846159246801 7 0.9512310150934811 2.655742414355247 8 0.6896517746854158 2.200501924175362 9 0.5236088944986456 1.8795393920842551 >10 2.49834925562904 17.66657835718772 >50 0.16550344657513066 4.536489924261431 >100 0.09367828632616826 6.760214542097362 >500 0.0046410824795646306 1.2811695457919197 >1k 0.0035741660530271744 2.651870437809474 >5k 2.6672880992734875E-4 0.7434675516966446 >10k+ 1.066915239709395E-4 0.564361297219858 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14295 0.30330175628584666 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12230 0.25948796637816757 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9318 0.1977030965422539 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7815 0.16581344703559928 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6173 0.13097458842620016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5957 0.12639164478452528 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5680 0.12051444391071069 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.2434663348841786E-5 2 0.0 0.0 0.0 0.0 4.2434663348841786E-5 3 0.0 0.0 0.0 0.0 4.2434663348841786E-5 4 0.0 0.0 0.0 2.1217331674420893E-5 4.2434663348841786E-5 5 0.0 0.0 0.0 2.1217331674420893E-5 4.2434663348841786E-5 6 2.1217331674420893E-5 0.0 0.0 2.1217331674420893E-5 4.2434663348841786E-5 7 4.2434663348841786E-5 0.0 0.0 2.1217331674420893E-5 4.2434663348841786E-5 8 4.2434663348841786E-5 0.0 0.0 2.1217331674420893E-5 4.2434663348841786E-5 9 4.2434663348841786E-5 0.0 0.0 6.365199502326268E-5 4.2434663348841786E-5 10-11 4.2434663348841786E-5 0.0 0.0 6.365199502326268E-5 4.2434663348841786E-5 12-13 4.2434663348841786E-5 0.0 0.0 1.0608665837210447E-4 1.2730399004652536E-4 14-15 4.2434663348841786E-5 0.0 0.0 1.0608665837210447E-4 1.379126558837358E-4 16-17 4.2434663348841786E-5 0.0 0.0 2.015646509069985E-4 1.9095598506978803E-4 18-19 6.365199502326268E-5 0.0 0.0 2.5460798009305073E-4 2.5460798009305073E-4 20-21 6.365199502326268E-5 0.0 0.0 3.606946384651552E-4 2.7582531176747164E-4 22-23 8.486932669768357E-5 0.0 0.0 5.092159601861015E-4 2.7582531176747164E-4 24-25 1.2730399004652536E-4 0.0 0.0 8.699105986512566E-4 2.7582531176747164E-4 26-27 1.2730399004652536E-4 0.0 0.0 0.0013579092271629374 2.970426434418925E-4 28-29 2.3339064841862984E-4 0.0 0.0 0.003575120387139921 2.970426434418925E-4 30-31 2.5460798009305073E-4 0.0 0.0 0.01116031646074539 2.970426434418925E-4 32-33 2.5460798009305073E-4 0.0 0.0 0.026033665964514438 2.970426434418925E-4 34-35 2.5460798009305073E-4 0.0 0.0 0.048237603561795905 2.970426434418925E-4 36-37 2.7582531176747164E-4 0.0 0.0 0.085092108680265 2.970426434418925E-4 38-39 2.7582531176747164E-4 0.0 0.0 0.1410528209715501 3.0765130927910296E-4 40-41 2.7582531176747164E-4 0.0 0.0 0.20394099205453364 3.182599751163134E-4 42-43 2.970426434418925E-4 0.0 0.0 0.26682916313751714 3.182599751163134E-4 44-45 3.394773067907343E-4 0.0 0.0 0.34709432886185143 3.182599751163134E-4 46-47 3.5008597262794474E-4 0.0 0.0 0.4460201377938388 3.182599751163134E-4 48-49 3.606946384651552E-4 0.0 0.0 0.5517354928616409 3.182599751163134E-4 50-51 3.7130330430236566E-4 0.0 0.0 0.6681868177567 3.394773067907343E-4 52-53 3.819119701395761E-4 0.0 0.0 0.783821275382294 4.243466334884179E-4 54-55 3.819119701395761E-4 0.0 0.0 0.9128863039577961 4.4556396516283876E-4 56-57 4.243466334884179E-4 0.0 0.0 1.0694808203808597 4.561726310000492E-4 58-59 4.243466334884179E-4 0.0 0.0 1.2383707805092499 4.667812968372597E-4 60-61 4.243466334884179E-4 0.0 0.0 1.4255712978726653 4.8799862851168054E-4 62-63 4.243466334884179E-4 0.0 0.0 1.6128248585652671 5.092159601861015E-4 64-65 4.243466334884179E-4 0.0 0.0 1.811588821691242 5.304332918605224E-4 66-67 4.8799862851168054E-4 0.0 0.0 2.0440459075161974 5.516506235349433E-4 68-69 5.092159601861015E-4 0.0 0.0 2.3054222164133886 5.516506235349433E-4 70-71 5.198246260233119E-4 0.0 0.0 2.5870610770596514 5.622592893721537E-4 72-73 5.410419576977329E-4 0.0 0.0 2.8755531358367525 6.365199502326268E-4 74-75 5.516506235349433E-4 0.0 0.0 3.1714818693657376 7.107806110931E-4 76-77 5.516506235349433E-4 0.0 0.0 3.4964889559545167 7.213892769303104E-4 78-79 5.516506235349433E-4 0.0 0.0 3.872661637876162 7.638239402791522E-4 80-81 5.94085286883785E-4 0.0 0.0 4.272183993305507 7.744326061163626E-4 82-83 6.153026185582059E-4 0.0 0.0 4.675790685082179 8.06258603627994E-4 84-85 6.471286160698372E-4 0.0 0.0 5.086579443630642 8.06258603627994E-4 86-87 6.89563279418679E-4 0.0 0.0 5.517588319264828 8.168672694652045E-4 88-89 7.001719452558895E-4 0.0 0.0 5.981123364355901 8.274759353024149E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16755 0.0 24.111008 1 TACATGG 17005 0.0 23.155361 2 TAAGGTG 2115 0.0 22.654844 5 GTAAGGT 1860 0.0 22.445011 4 ACATGGG 16760 0.0 22.361616 3 GAGTACT 13535 0.0 21.165808 12-13 CATGGGG 10840 0.0 20.044088 4 GTACTTT 14240 0.0 19.88468 14-15 AGAGTAC 22165 0.0 19.583704 10-11 AGGTAAG 2055 0.0 19.391775 2 GTATCAA 32925 0.0 19.32839 1 AGTACTT 14250 0.0 19.104443 12-13 GGTAAGG 2190 0.0 18.846264 3 GTGTACG 505 0.0 17.881464 1 CAACGCA 36235 0.0 17.28207 5 TCAACGC 36620 0.0 17.165152 4 ATCAACG 36765 0.0 17.097452 3 TATCAAC 37085 0.0 17.026676 2 AACGCAG 36895 0.0 17.010769 6 ACTTTTT 17350 0.0 16.82659 16-17 >>END_MODULE