FastQCFastQC Report
Thu 26 May 2016
SRR938730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4713128
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT154190.3271500370878958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118470.2513617283468643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97230.20629611587039434No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT72720.15429243593638875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63570.13487857745429363No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57670.1223603517663853No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54720.11610123892243113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG170250.023.359431
TACATGG169600.022.6403372
ACATGGG167250.022.1638453
GAGTACT138850.020.28070312-13
GTAAGGT22000.019.632654
TAAGGTG24000.019.5787145
GTACTTT144550.019.15273914-15
AGAGTAC230350.019.06322310-11
AGTACTT146300.019.05336412-13
GGTAAGG24500.018.5979633
GTATCAA328050.018.1554621
GTATAGG11850.018.0649681
CATGGGG112650.017.78044
TATAGGG15300.017.0620612
AGGTAAG24800.017.0332892
TACTTTT164950.016.6114614-15
CATGGGA60800.016.1594664
TAGACCG4701.4551915E-1116.1578045
TCAACGC367850.015.9738684
CAACGCA368850.015.9176935
Produced by FastQC (version 0.11.5)