##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938730_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4713128 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08882211558863 33.0 31.0 34.0 30.0 34.0 2 32.29689518298675 34.0 31.0 34.0 30.0 34.0 3 32.38212838692265 34.0 31.0 34.0 30.0 34.0 4 35.98798950505906 37.0 35.0 37.0 35.0 37.0 5 35.88900980410462 37.0 35.0 37.0 35.0 37.0 6 35.91259180739416 37.0 35.0 37.0 35.0 37.0 7 35.83392918673119 37.0 35.0 37.0 35.0 37.0 8 35.81316145031495 37.0 35.0 37.0 33.0 37.0 9 37.47065070161472 39.0 37.0 39.0 34.0 39.0 10-11 37.3953978122385 39.0 37.0 39.0 34.0 39.0 12-13 37.31778841991985 39.0 37.0 39.0 34.0 39.0 14-15 38.553188880081336 40.0 38.0 41.0 34.0 41.0 16-17 38.47366091054603 40.0 38.0 41.0 33.5 41.0 18-19 38.611323838435965 40.0 38.0 41.0 34.0 41.0 20-21 38.60481266369172 40.0 38.0 41.0 34.0 41.0 22-23 38.53438608075147 40.0 38.0 41.0 34.0 41.0 24-25 38.45020875732635 40.0 38.0 41.0 34.0 41.0 26-27 38.27710545947404 40.0 38.0 41.0 33.5 41.0 28-29 38.115395868730914 40.0 38.0 41.0 33.0 41.0 30-31 37.906550702633155 40.0 37.0 41.0 33.0 41.0 32-33 37.77170225378984 40.0 37.0 41.0 33.0 41.0 34-35 37.75810470243965 40.0 37.0 41.0 33.0 41.0 36-37 37.65389694487398 40.0 37.0 41.0 32.5 41.0 38-39 37.53211190105594 40.0 37.0 41.0 32.0 41.0 40-41 37.37063527661459 40.0 37.0 41.0 31.5 41.0 42-43 37.18349310691329 40.0 36.0 41.0 31.0 41.0 44-45 36.959575784913966 40.0 36.0 41.0 31.0 41.0 46-47 36.690544899268595 39.0 36.0 41.0 30.0 41.0 48-49 36.400030722696265 39.0 35.0 41.0 30.0 41.0 50-51 36.167263969915524 39.0 35.0 41.0 29.5 41.0 52-53 35.93916163533008 39.0 35.0 40.0 29.0 41.0 54-55 35.65528455836548 38.5 35.0 40.0 28.0 41.0 56-57 35.36458366927442 38.0 34.5 40.0 28.0 41.0 58-59 35.04058069290713 38.0 34.0 40.0 27.0 41.0 60-61 34.653311664779736 37.5 34.0 40.0 26.0 41.0 62-63 34.28176648289629 37.0 33.5 40.0 26.0 41.0 64-65 33.869904763885046 36.0 33.0 39.0 26.0 41.0 66-67 33.44679669213312 36.0 33.0 39.0 25.0 40.0 68-69 33.08478742779742 35.0 32.0 38.5 24.5 40.0 70-71 32.63279057984421 35.0 32.0 38.0 23.5 40.0 72-73 32.14767878148015 35.0 31.5 37.0 22.0 39.0 74-75 31.66063461463385 35.0 31.0 36.5 20.5 39.0 76-77 29.749449622416364 32.5 28.5 35.0 18.5 37.0 78-79 30.711033309513343 34.0 30.5 35.5 19.0 37.0 80-81 30.77432662554465 34.0 31.0 35.0 19.0 37.0 82-83 30.463864550251976 34.0 30.5 35.0 18.0 37.0 84-85 30.07117470605509 34.0 30.0 35.0 14.5 36.0 86-87 29.509922921677493 34.0 29.5 35.0 7.0 36.0 88-89 28.90207543694973 34.0 29.0 35.0 2.0 35.5 90-91 28.381257733717398 33.5 28.5 35.0 2.0 35.0 92-93 28.088240972874065 33.0 27.5 35.0 2.0 35.0 94-95 27.738679917031746 33.0 27.0 35.0 2.0 35.0 96-97 27.105745801938752 33.0 26.5 35.0 2.0 35.0 98-99 26.240508320588788 33.0 25.0 35.0 2.0 35.0 100-101 22.445568632975807 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11400252858717863 1101 2 0.1259350239551864 1101 3 0.10226313953536703 1101 4 0.10200457288851794 1101 5 -0.06578871550144072 1101 6 0.029592508658268457 1101 7 0.1320946910875591 1101 8 0.14419662534795918 1101 9 0.27573376362035873 1101 10-11 0.27229861075426953 1101 12-13 0.16184272065655847 1101 14-15 0.2612469171519578 1101 16-17 0.3223981071738109 1101 18-19 0.25948056304147116 1101 20-21 0.2853304663324252 1101 22-23 0.1884983656855681 1101 24-25 0.20577807914937551 1101 26-27 0.10570578675267228 1101 28-29 0.16879821686284657 1101 30-31 0.23994477344832887 1101 32-33 0.20835743720935085 1101 34-35 0.26753172413185666 1101 36-37 0.27341086297874995 1101 38-39 0.3284142381559292 1101 40-41 0.3356440993351484 1101 42-43 0.4503152651596025 1101 44-45 0.42710840396716065 1101 46-47 0.4487255794726721 1101 48-49 0.5158293061424573 1101 50-51 0.42572422967778323 1101 52-53 0.3732426646262539 1101 54-55 0.36284345308158095 1101 56-57 0.48955815802068514 1101 58-59 0.49286748592722773 1101 60-61 0.563453787894808 1101 62-63 0.4191043566705659 1101 64-65 0.46014113916178445 1101 66-67 0.4773407211626548 1101 68-69 0.4379596105541097 1101 70-71 0.5391446018667949 1101 72-73 0.44052850018386636 1101 74-75 0.30800215491765925 1101 76-77 0.36312033330913707 1101 78-79 0.28701931985884244 1101 80-81 0.41500634678279624 1101 82-83 0.4679368787903506 1101 84-85 0.5601348495287048 1101 86-87 0.5308834894896926 1101 88-89 0.5565248574516986 1101 90-91 0.4694636508849932 1101 92-93 0.5666183288672144 1101 94-95 0.6076016943417493 1101 96-97 0.5618516474399478 1101 98-99 0.36240460066319713 1101 100-101 1.049921592965724 1102 1 0.3219270432080279 1102 2 0.20491754106125626 1102 3 0.19605335461668005 1102 4 0.12885003390366023 1102 5 -0.011509834286748344 1102 6 0.04023004149206599 1102 7 0.10284715921673637 1102 8 0.12653199941178173 1102 9 0.2544943110090756 1102 10-11 0.26985180586647317 1102 12-13 0.2858254969541676 1102 14-15 0.32552481705965164 1102 16-17 0.346448172439203 1102 18-19 0.28625548545473833 1102 20-21 0.30953771946989406 1102 22-23 0.2719739258933842 1102 24-25 0.32950537645618994 1102 26-27 0.2653739684027272 1102 28-29 0.38454752038008877 1102 30-31 0.3355575795000405 1102 32-33 0.3752691403425814 1102 34-35 0.3710287887589132 1102 36-37 0.2794844016024811 1102 38-39 0.3273745156646015 1102 40-41 0.4441462879797555 1102 42-43 0.35555345990123755 1102 44-45 0.30282976888131685 1102 46-47 0.4318294301240755 1102 48-49 0.4185317091895797 1102 50-51 0.3049959229676915 1102 52-53 0.23852365186967717 1102 54-55 0.27293105065825785 1102 56-57 0.28745119885823556 1102 58-59 0.31520666696717825 1102 60-61 0.25087033841416684 1102 62-63 0.24861131842722983 1102 64-65 0.2585684445949852 1102 66-67 0.38888827071166787 1102 68-69 0.348188629089897 1102 70-71 0.3884842546766194 1102 72-73 0.3668940145351769 1102 74-75 0.34572781714061307 1102 76-77 0.28424641998288536 1102 78-79 0.17007296389264326 1102 80-81 0.37201962321102044 1102 82-83 0.4861258462505127 1102 84-85 0.6546854514879037 1102 86-87 0.6492223297076585 1102 88-89 0.5459974867553932 1102 90-91 0.5727030135698676 1102 92-93 0.6701025828775009 1102 94-95 0.7900453421802318 1102 96-97 0.832960301133042 1102 98-99 1.0249531773074203 1102 100-101 1.752888238473865 1103 1 0.2020380734771905 1103 2 0.13961077131024524 1103 3 0.14671609264900098 1103 4 0.10863056559910689 1103 5 0.01947203153303434 1103 6 0.032430562679302 1103 7 0.0996141001046027 1103 8 0.08532788211791598 1103 9 0.1849033021516746 1103 10-11 0.17196705963470293 1103 12-13 0.1569412514233619 1103 14-15 0.19419526844751545 1103 16-17 0.12064875247958895 1103 18-19 0.032992202334511944 1103 20-21 0.17276084093716548 1103 22-23 0.1281605478556216 1103 24-25 0.16907126490612967 1103 26-27 0.22465374972062335 1103 28-29 0.12822665934515953 1103 30-31 0.18173006996953234 1103 32-33 0.24154175303342384 1103 34-35 0.21786072642670007 1103 36-37 0.31043484164202795 1103 38-39 0.2660039652821595 1103 40-41 0.3712893790590286 1103 42-43 0.3349643848861703 1103 44-45 0.42347214866872207 1103 46-47 0.3759361978084854 1103 48-49 0.47323307554835736 1103 50-51 0.5017957272697657 1103 52-53 0.5012042686775544 1103 54-55 0.46772104265917847 1103 56-57 0.2760446065426976 1103 58-59 0.41966807199830924 1103 60-61 0.48088833804083464 1103 62-63 0.4665248667572257 1103 64-65 0.49907535433560923 1103 66-67 0.4525121551133111 1103 68-69 0.4717906255142381 1103 70-71 0.44222530400116966 1103 72-73 0.4720225940472247 1103 74-75 0.5399846715660175 1103 76-77 0.5608507464974153 1103 78-79 0.42901108251047404 1103 80-81 0.6224870227265811 1103 82-83 0.5125338121803864 1103 84-85 0.5198422580445872 1103 86-87 0.737890786378383 1103 88-89 0.6854548563030249 1103 90-91 0.6468282975306572 1103 92-93 0.741696283255262 1103 94-95 0.788888608403763 1103 96-97 0.7777602105621 1103 98-99 0.8119854492700327 1103 100-101 1.4985146077359914 1104 1 0.07676361424263689 1104 2 0.05406311542345321 1104 3 0.0443887953976585 1104 4 0.05122122488433689 1104 5 -0.12745783163142477 1104 6 -0.006837809112724358 1104 7 0.017017805201732017 1104 8 -0.010254276962129438 1104 9 0.028917564565276166 1104 10-11 0.06809730580831541 1104 12-13 -0.034293789082362025 1104 14-15 -0.04842161796463529 1104 16-17 0.012733281423315645 1104 18-19 0.14360279334940884 1104 20-21 0.08715423498234287 1104 22-23 0.054207144348282554 1104 24-25 0.136328703333902 1104 26-27 0.117110956604769 1104 28-29 0.062045419921638256 1104 30-31 -0.09416522726051824 1104 32-33 -0.0798907979127037 1104 34-35 -0.06049014375744122 1104 36-37 0.0851249325546064 1104 38-39 0.11176954797553407 1104 40-41 0.14928466438379218 1104 42-43 0.02897180785036113 1104 44-45 0.06069724326263071 1104 46-47 -0.05448030803319881 1104 48-49 0.07532778717965982 1104 50-51 0.01122643012351432 1104 52-53 -0.01232762078187477 1104 54-55 -0.004989842396618371 1104 56-57 -0.022589654066266007 1104 58-59 0.1928648350875477 1104 60-61 0.2876618981704553 1104 62-63 0.13455854389574995 1104 64-65 0.054781860591880616 1104 66-67 -0.03717592830446392 1104 68-69 0.07978688410262436 1104 70-71 0.041978263531802895 1104 72-73 0.010895763164306516 1104 74-75 -0.024808436752017826 1104 76-77 -0.0910283506597871 1104 78-79 -0.056988287417716776 1104 80-81 0.15131302957223625 1104 82-83 0.19040212983323812 1104 84-85 0.1668083248423997 1104 86-87 0.24930872063409382 1104 88-89 0.281287337014561 1104 90-91 0.25643070714517435 1104 92-93 0.33008010473166394 1104 94-95 0.27498545988484224 1104 96-97 0.41736190851915467 1104 98-99 0.6867185500103545 1104 100-101 1.546596779563803 1105 1 0.06384874414453634 1105 2 0.04846132515396562 1105 3 0.035352327953745544 1105 4 0.025038082688730867 1105 5 -0.09458576523659445 1105 6 -0.05211589008870021 1105 7 0.022040926798553073 1105 8 0.05118437878164883 1105 9 0.10612592812929478 1105 10-11 0.04041964173396195 1105 12-13 0.01592734517240757 1105 14-15 0.06479212181944405 1105 16-17 -0.003905014795719808 1105 18-19 0.07782780834839542 1105 20-21 -0.004576782647170319 1105 22-23 -0.036631197175587715 1105 24-25 -0.0358034589538434 1105 26-27 0.02040432067786213 1105 28-29 0.11976728396238201 1105 30-31 0.02878161552840197 1105 32-33 0.0768474551349172 1105 34-35 0.0351479172649789 1105 36-37 0.014961692051663533 1105 38-39 0.06424046971212505 1105 40-41 0.055590704618019515 1105 42-43 0.14738836222336005 1105 44-45 0.14158332917981653 1105 46-47 0.06383931012265975 1105 48-49 0.07239088647789771 1105 50-51 0.0593153578610881 1105 52-53 0.02788330427452479 1105 54-55 0.04143941849106625 1105 56-57 -0.007723029281706317 1105 58-59 0.1290778772604213 1105 60-61 0.04420336917961265 1105 62-63 0.10693230740069737 1105 64-65 0.07055911343639565 1105 66-67 0.016476544234677704 1105 68-69 -0.014046008179235514 1105 70-71 0.032869944908377136 1105 72-73 0.14658376704401377 1105 74-75 0.19203805443877897 1105 76-77 0.2040828074944976 1105 78-79 -0.09715559009235264 1105 80-81 -0.025530003048803707 1105 82-83 0.12501753479550715 1105 84-85 0.29076609247958984 1105 86-87 0.29810642535098353 1105 88-89 0.20287346069155632 1105 90-91 0.1309613112379786 1105 92-93 0.23366991454328456 1105 94-95 0.452062605069667 1105 96-97 0.5223082096273721 1105 98-99 0.6526796933087518 1105 100-101 1.35712183693634 1106 1 0.08222753176841024 1106 2 0.02779339103884837 1106 3 0.013755511156126943 1106 4 0.021938666500162185 1106 5 -0.09023292104863145 1106 6 -0.03452348131974503 1106 7 -0.04423613967519202 1106 8 0.052985781948031274 1106 9 0.07130553006075502 1106 10-11 0.022798547129589508 1106 12-13 0.025341178306653944 1106 14-15 0.0896417104595173 1106 16-17 0.08424706219336286 1106 18-19 -0.12906183951405126 1106 20-21 -0.06790677911401843 1106 22-23 0.02937019926097406 1106 24-25 0.017580544276462717 1106 26-27 0.033955055068901174 1106 28-29 0.05885901133758864 1106 30-31 0.04079374601951713 1106 32-33 0.021090687464948132 1106 34-35 0.09244589663809677 1106 36-37 0.03675653364864928 1106 38-39 0.16642976257683273 1106 40-41 0.1725341909985545 1106 42-43 0.05947149226020798 1106 44-45 -0.029046076745785854 1106 46-47 0.08605619522789709 1106 48-49 0.00989648063642079 1106 50-51 0.02465899902735913 1106 52-53 -0.0695009849729118 1106 54-55 0.020968499703116095 1106 56-57 0.02976044061078653 1106 58-59 0.08356228932697007 1106 60-61 0.046760362298378766 1106 62-63 0.17320676607121754 1106 64-65 0.042744200166886515 1106 66-67 -0.019644895272691087 1106 68-69 -0.013809791681090644 1106 70-71 -0.013068023024061404 1106 72-73 -0.08643539610352491 1106 74-75 0.10028939619559907 1106 76-77 0.06129439287061089 1106 78-79 -0.01000743322037323 1106 80-81 -0.003047374383161383 1106 82-83 0.06768069074214011 1106 84-85 0.055903633068783876 1106 86-87 0.18621383319458218 1106 88-89 0.3061621529203009 1106 90-91 0.1753292975091547 1106 92-93 0.31028523833149535 1106 94-95 0.5481192701099609 1106 96-97 0.6701496215425067 1106 98-99 0.7838603551860324 1106 100-101 1.2899778020758994 1107 1 0.04866611372781193 1107 2 0.09815489399206001 1107 3 0.05225505537519837 1107 4 0.0561901659985935 1107 5 0.0030915741583825707 1107 6 -0.05015984722650302 1107 7 0.026045112536074555 1107 8 -0.040149283214077514 1107 9 0.020380731832261745 1107 10-11 -3.122721522359484E-4 1107 12-13 0.07539568471047886 1107 14-15 0.06792121030758835 1107 16-17 0.09511048608626282 1107 18-19 0.11670824294276372 1107 20-21 0.06789302275655729 1107 22-23 0.10559570884068847 1107 24-25 0.06255962830248762 1107 26-27 0.08413158172687929 1107 28-29 0.15898869037624763 1107 30-31 0.14075528560169204 1107 32-33 0.10666289023959763 1107 34-35 0.11108257056118731 1107 36-37 0.08353591070643063 1107 38-39 0.1045967324037278 1107 40-41 -0.013520140585185914 1107 42-43 0.07592732729664675 1107 44-45 0.06775825379735778 1107 46-47 0.0645552815848518 1107 48-49 0.1286347167018036 1107 50-51 -0.0223932222282599 1107 52-53 -0.00560526515123172 1107 54-55 0.08987624881132206 1107 56-57 0.075183775761694 1107 58-59 0.1984939430000665 1107 60-61 0.16878267683299697 1107 62-63 0.12925489570447724 1107 64-65 0.10212802181710146 1107 66-67 0.05166015744388375 1107 68-69 0.02977508138703655 1107 70-71 0.03701112468642975 1107 72-73 -0.0038916598653955248 1107 74-75 0.11274778277274322 1107 76-77 0.08728218876400362 1107 78-79 0.1703457033432514 1107 80-81 0.26873884979445606 1107 82-83 0.32901050963685563 1107 84-85 0.23212797718130318 1107 86-87 0.2554201500268398 1107 88-89 0.26063080393363336 1107 90-91 0.3787159162806688 1107 92-93 0.4866471283863163 1107 94-95 0.6478549796089368 1107 96-97 0.72063308231413 1107 98-99 0.9154633096454035 1107 100-101 1.7347337823795037 1108 1 0.12419217932956172 1108 2 0.05784730622194445 1108 3 0.06848756355154961 1108 4 0.08230955953823127 1108 5 -0.013708098353596654 1108 6 0.09173683131594146 1108 7 0.09646095831293877 1108 8 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-1.7728772757995088 2316 100-101 -1.8775735973139405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 19.0 4 49.0 5 100.0 6 268.0 7 727.0 8 1726.0 9 3068.0 10 5006.0 11 7403.0 12 10314.0 13 13538.0 14 16672.0 15 21792.0 16 27294.0 17 35431.0 18 34345.0 19 27930.0 20 27051.0 21 28310.0 22 30757.0 23 34473.0 24 40403.0 25 48006.0 26 57525.0 27 70674.0 28 85577.0 29 103289.0 30 125485.0 31 152340.0 32 187667.0 33 237546.0 34 316500.0 35 445282.0 36 680187.0 37 1009553.0 38 761672.0 39 65140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53227729796901 19.25901995942568 14.71553846680628 23.493164275799028 2 15.860443425258131 20.703405466603073 38.753180478017995 24.682970630120803 3 19.882231078807962 23.93508090592914 29.566309253642164 26.616378761620734 4 13.541495159902297 16.673512792353613 36.09165717544696 33.69333487229713 5 13.962807388095749 37.32060980568768 34.04092622006555 14.675656586151014 6 32.89134332019146 35.74549319350798 16.758523949129703 14.604639537170849 7 28.883917432329444 32.402387543898655 20.4102455948576 18.303449428914302 8 26.5643962990184 34.66888656535532 19.308408343673246 19.45830879195303 9 26.164789074262355 16.958610078062808 20.07142602534877 36.805174822326066 10-11 25.44431214259405 26.128581697759962 26.881478712226787 21.545627447419207 12-13 26.43033458883357 23.32781965607554 27.301030228756783 22.940815526334106 14-15 22.936816059313475 25.00004243466335 25.6732895860244 26.38985191999878 16-17 22.56426044748465 28.008757076322166 26.460514384874905 22.96646809131828 18-19 22.565650667666993 26.93686655656286 28.2622177882714 22.23526498749875 20-21 23.255510989729114 25.85873967352467 28.29157196664296 22.594177370103253 22-23 22.895580175204238 25.750202413344176 28.39941966354404 22.954797747907545 24-25 23.08116002698428 26.013064185052155 28.331265875250857 22.57450991271271 26-27 22.66890314192169 26.011256779284675 28.522294699146265 22.797545379647367 28-29 22.46249200106596 26.76839033440212 28.22534206582126 22.543775598710667 30-31 22.683417467125867 26.079272618948607 28.088405407194543 23.148904506730986 32-33 22.584385571535506 26.13967836222568 28.196433451414855 23.079502614823955 34-35 23.23956968585166 26.221614608503096 27.953423338767834 22.585392366877407 36-37 23.274059376525262 25.802729028287676 28.2512042951425 22.672007300044562 38-39 22.758550370229216 26.317287733056393 28.132189676531667 22.791972220182725 40-41 22.983843245830844 26.249664735998696 28.084556127437665 22.68193589073279 42-43 22.751391067672422 26.286134180341104 27.648322729821906 23.314152022164567 44-45 22.867240856905944 26.14922566643949 27.89804318139189 23.085490295262677 46-47 23.428032868815947 26.57377591964693 27.41322350610251 22.584967705434607 48-49 23.152143850812514 26.617649570623016 27.297655159361256 22.932551419203214 50-51 22.803305067592085 26.739312719413828 27.633772150373236 22.82361006262086 52-53 23.3256130535814 26.70239382422884 27.038126271979035 22.93386685021073 54-55 22.763396198872595 26.556450408306333 27.324454162925342 23.35569922989573 56-57 22.978060466988858 26.738413994707265 27.138620441994522 23.14490509630936 58-59 23.26098928232745 26.38778248769627 27.072087426045282 23.279140803930996 60-61 22.73500615689402 26.63723678044782 27.71096684613091 22.916790216527254 62-63 22.716335583344126 26.564512845451553 27.729258637939857 22.98989293326446 64-65 22.508695786398576 26.931577445940892 27.28437383070887 23.275352936951663 66-67 22.566289764549076 26.721789710919115 27.09705092491264 23.61486959961917 68-69 22.695249440471315 26.479198433054464 27.397921355043376 23.42763077143085 70-71 22.888934034708413 26.495990715284822 27.077462977039108 23.537612272967657 72-73 22.73019107480213 26.392525303789753 27.29194921080013 23.585334410607985 74-75 22.46672925094176 27.167859279374145 26.88882917367858 23.476582296005517 76-77 22.694810288601307 26.521541230896545 27.068476387792895 23.71517209270925 78-79 22.72564777933746 26.647663645029702 27.164455036068098 23.46223353956474 80-81 22.996036029536963 26.518999528439753 26.9263978790928 23.558566562930483 82-83 22.82279258943904 26.545333528393133 27.200488342612992 23.431385539554828 84-85 22.51806019272127 26.837537618329062 27.04141495838857 23.602987230561105 86-87 22.684095991986545 26.66384952423817 26.95013392400576 23.70192055976953 88-89 22.480745603481868 26.706566616911502 26.64996923129337 24.162718548313258 90-91 22.49820349868033 26.524709601565988 26.85956658387193 24.11752031588175 92-93 22.70252407512021 26.550575654623138 26.89602677636063 23.850873493896017 94-95 22.43503480019974 26.25194417412926 27.121209211860638 24.191811813810364 96-97 22.327237483329796 25.95022686118306 27.39991273305002 24.322622922437123 98-99 22.207396022344398 26.351310637012194 26.84715967824341 24.594133662400004 100-101 22.327338702294053 25.44032525662367 27.53832765569256 24.69400838538972 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 807.0 1 573.5 2 424.5 3 559.5 4 524.5 5 500.5 6 871.0 7 1888.0 8 3030.5 9 3563.5 10 3306.0 11 2925.0 12 3045.0 13 3261.5 14 3612.5 15 3933.5 16 4110.0 17 4458.0 18 4929.0 19 5471.5 20 6284.5 21 7588.0 22 9343.5 23 11512.5 24 14012.0 25 17455.5 26 22772.0 27 29233.0 28 34713.5 29 40875.0 30 48164.0 31 57917.0 32 70161.0 33 76805.0 34 80747.5 35 87783.0 36 98280.5 37 107239.0 38 113698.5 39 119434.5 40 124023.0 41 130368.0 42 134442.0 43 138833.5 44 146230.5 45 155221.5 46 162045.5 47 176683.5 48 202112.0 49 200445.5 50 179417.0 51 172793.0 52 173606.0 53 176713.5 54 178536.0 55 169673.0 56 155692.5 57 141847.0 58 132628.5 59 120630.5 60 99570.0 61 81575.0 62 67105.5 63 50601.0 64 35382.5 65 25775.0 66 19094.5 67 14670.0 68 11188.0 69 8962.0 70 7013.5 71 4442.5 72 2925.5 73 2230.0 74 1709.5 75 1220.5 76 828.5 77 515.0 78 302.0 79 201.0 80 154.5 81 104.5 82 70.0 83 47.0 84 28.0 85 17.0 86 12.0 87 8.5 88 10.0 89 7.5 90 2.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23633985752137435 2 0.0 3 0.0 4 0.0 5 0.028261485790328632 6 0.0042646836665586 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0029598177685817148 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.8643397760468205E-4 26-27 0.0012093879054419908 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007182066771791474 36-37 0.017567950626420498 38-39 0.013621526934978215 40-41 0.008996148629954459 42-43 0.008083803367954362 44-45 0.0036493810480003937 46-47 6.046939527209954E-4 48-49 0.0016655605364420403 50-51 4.2434663348841786E-5 52-53 0.0 54-55 0.0 56-57 0.001283648566302464 58-59 5.198246260233119E-4 60-61 0.002514253803418876 62-63 8.380846011396253E-4 64-65 0.0014746045513722521 66-67 0.005887809539651798 68-69 2.0156465090699852E-4 70-71 1.1669532420931493E-4 72-73 0.0 74-75 3.5008597262794474E-4 76-77 0.05917513803995987 78-79 0.14180603624599206 80-81 0.0010714752495582553 82-83 9.22953927837309E-4 84-85 0.0 86-87 0.10709448162663947 88-89 0.3564087374669222 90-91 0.394355935166624 92-93 3.288686409535238E-4 94-95 1.803473192325776E-4 96-97 7.426066086047313E-5 98-99 0.0 100-101 0.0010502579178838342 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4713128.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.82294696447538 #Duplication Level Percentage of deduplicated Percentage of total 1 63.31633535622093 23.314940588070606 2 16.055474639799233 11.824197823016132 3 7.144732251093712 7.892702902722015 4 3.8766331915857677 5.709962336579505 5 2.383291738257009 4.387991263935509 6 1.5501451652428258 3.42485479241847 7 1.0791665695684058 2.7816665349936565 8 0.7953512487491318 2.3429741480654833 9 0.5752889021207616 1.9065449458839663 >10 2.91416158251104 18.845695402205415 >50 0.19235608477795302 4.897889554564527 >100 0.10719629342582837 7.149285144219577 >500 0.0053662510173202935 1.3643616866438208 >1k 0.004096814355650255 2.8426303836181614 >5k 2.8850805321476976E-4 0.7349699716693618 >10k+ 1.1540322128590788E-4 0.5793325213939122 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15419 0.3271500370878958 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11847 0.2513617283468643 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9723 0.20629611587039434 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7272 0.15429243593638875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6357 0.13487857745429363 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5767 0.1223603517663853 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5472 0.11610123892243113 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.1217331674420893E-5 7 0.0 0.0 0.0 0.0 2.1217331674420893E-5 8 0.0 0.0 0.0 0.0 2.1217331674420893E-5 9 0.0 0.0 0.0 4.2434663348841786E-5 2.1217331674420893E-5 10-11 1.0608665837210446E-5 0.0 0.0 4.2434663348841786E-5 2.1217331674420893E-5 12-13 2.1217331674420893E-5 0.0 0.0 6.365199502326268E-5 8.486932669768357E-5 14-15 4.2434663348841786E-5 0.0 0.0 6.365199502326268E-5 8.486932669768357E-5 16-17 4.2434663348841786E-5 0.0 0.0 1.591299875581567E-4 1.0608665837210447E-4 18-19 5.304332918605224E-5 0.0 0.0 2.1217331674420895E-4 1.2730399004652536E-4 20-21 6.365199502326268E-5 0.0 0.0 3.288686409535238E-4 1.6973865339536714E-4 22-23 6.365199502326268E-5 0.0 0.0 4.561726310000492E-4 1.6973865339536714E-4 24-25 1.0608665837210447E-4 0.0 0.0 7.850412719535732E-4 1.6973865339536714E-4 26-27 1.6973865339536714E-4 0.0 0.0 0.0012942572321396745 1.9095598506978806E-4 28-29 2.015646509069985E-4 0.0 0.0 0.003532685723791079 1.9095598506978806E-4 30-31 2.3339064841862984E-4 0.0 0.0 0.011425533106675653 1.9095598506978806E-4 32-33 2.3339064841862984E-4 1.0608665837210446E-5 0.0 0.02657470792221217 2.1217331674420895E-4 34-35 2.7582531176747164E-4 2.1217331674420893E-5 0.0 0.0492560354821681 2.1217331674420895E-4 36-37 3.394773067907343E-4 2.1217331674420893E-5 0.0 0.08636514858073024 2.1217331674420895E-4 38-39 3.606946384651552E-4 2.1217331674420893E-5 0.0 0.14249559952541072 2.1217331674420895E-4 40-41 3.606946384651552E-4 2.1217331674420893E-5 0.0 0.20590359523441756 2.1217331674420895E-4 42-43 3.925206359767866E-4 2.1217331674420893E-5 0.0 0.26929037361175 2.3339064841862984E-4 44-45 4.243466334884179E-4 2.1217331674420893E-5 0.0 0.35037240660554947 2.4399931425584027E-4 46-47 5.304332918605224E-4 2.1217331674420893E-5 0.0 0.4505288207746533 2.5460798009305073E-4 48-49 5.94085286883785E-4 2.1217331674420893E-5 0.0 0.5575490417404323 2.652166459302612E-4 50-51 5.94085286883785E-4 2.1217331674420893E-5 0.0 0.6753264498651426 2.970426434418925E-4 52-53 5.94085286883785E-4 2.1217331674420893E-5 0.0 0.7922127300595273 2.970426434418925E-4 54-55 6.153026185582059E-4 2.1217331674420893E-5 0.0 0.9230175798323321 2.970426434418925E-4 56-57 6.365199502326268E-4 2.1217331674420893E-5 0.0 1.0813625261185353 3.0765130927910296E-4 58-59 6.365199502326268E-4 2.1217331674420893E-5 0.0 1.2519180467833677 3.182599751163134E-4 60-61 6.365199502326268E-4 2.1217331674420893E-5 0.0 1.4413994273017834 3.182599751163134E-4 62-63 6.365199502326268E-4 2.1217331674420893E-5 0.0 1.6302973311991527 3.606946384651552E-4 64-65 6.365199502326268E-4 2.1217331674420893E-5 0.0 1.83034494289143 3.606946384651552E-4 66-67 6.365199502326268E-4 2.1217331674420893E-5 0.0 2.064552458579525 3.606946384651552E-4 68-69 6.365199502326268E-4 2.1217331674420893E-5 0.0 2.327923196654112 3.606946384651552E-4 70-71 6.683459477442582E-4 2.1217331674420893E-5 0.0 2.612861352375747 3.606946384651552E-4 72-73 7.107806110931E-4 2.1217331674420893E-5 0.0 2.9044193155798017 3.7130330430236566E-4 74-75 7.213892769303104E-4 2.1217331674420893E-5 0.0 3.20391256083009 3.819119701395761E-4 76-77 7.213892769303104E-4 2.1217331674420893E-5 0.0 3.5315399878806604 3.819119701395761E-4 78-79 7.213892769303104E-4 3.182599751163134E-5 0.0 3.9110331822093523 3.819119701395761E-4 80-81 7.532152744419418E-4 4.2434663348841786E-5 0.0 4.314544395993488 3.819119701395761E-4 82-83 7.85041271953573E-4 4.2434663348841786E-5 0.0 4.722596118755952 3.819119701395761E-4 84-85 8.06258603627994E-4 4.2434663348841786E-5 0.0 5.1368220850356705 3.819119701395761E-4 86-87 8.274759353024149E-4 4.2434663348841786E-5 0.0 5.571681906368764 4.03129301813997E-4 88-89 8.274759353024149E-4 5.304332918605224E-5 2.1217331674420893E-5 6.04007572041328 4.243466334884179E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 17025 0.0 23.35943 1 TACATGG 16960 0.0 22.640337 2 ACATGGG 16725 0.0 22.163845 3 GAGTACT 13885 0.0 20.280703 12-13 GTAAGGT 2200 0.0 19.63265 4 TAAGGTG 2400 0.0 19.578714 5 GTACTTT 14455 0.0 19.152739 14-15 AGAGTAC 23035 0.0 19.063223 10-11 AGTACTT 14630 0.0 19.053364 12-13 GGTAAGG 2450 0.0 18.597963 3 GTATCAA 32805 0.0 18.155462 1 GTATAGG 1185 0.0 18.064968 1 CATGGGG 11265 0.0 17.7804 4 TATAGGG 1530 0.0 17.062061 2 AGGTAAG 2480 0.0 17.033289 2 TACTTTT 16495 0.0 16.61146 14-15 CATGGGA 6080 0.0 16.159466 4 TAGACCG 470 1.4551915E-11 16.157804 5 TCAACGC 36785 0.0 15.973868 4 CAACGCA 36885 0.0 15.917693 5 >>END_MODULE