FastQCFastQC Report
Thu 26 May 2016
SRR938729_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938729_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4047351
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155590.38442428146212176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117610.29058512592557456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80800.19963675006195414No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63010.15568207452232335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG183300.025.5771471
GTATCAA260200.024.8681561
TACATGG187050.024.3173832
ACATGGG188250.023.4324683
GTATAGG11350.022.1359541
GGTATCA191200.021.3964211
GAGTACT137550.021.24036812-13
TCAACGC301150.021.2399864
CAACGCA302350.021.1246075
TACCTGG30750.021.109542
ATCAACG303150.021.0373383
CATGGGG74100.020.9729234
TATCAAC306200.020.9051592
AACGCAG308050.020.8108256
AGTACTT144250.019.9091412-13
GTACTTT146450.019.83663714-15
CATGGGA110200.018.960964
ACGCAGA338600.018.8646627
CGCAGAG339550.018.7282128
AGAGTAC258200.017.93566310-11
Produced by FastQC (version 0.11.5)