##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938729_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4047351 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41824739193611 33.0 31.0 34.0 28.0 34.0 2 31.476743183380933 33.0 31.0 34.0 28.0 34.0 3 31.487515908553522 33.0 31.0 34.0 28.0 34.0 4 35.03695478845299 37.0 35.0 37.0 32.0 37.0 5 35.072825410991044 37.0 35.0 37.0 32.0 37.0 6 35.15163473590504 37.0 35.0 37.0 32.0 37.0 7 35.058149886184815 37.0 35.0 37.0 32.0 37.0 8 35.023812612249344 37.0 35.0 37.0 32.0 37.0 9 36.564729868993325 39.0 37.0 39.0 32.0 39.0 10-11 36.45049268027408 39.0 37.0 39.0 32.0 39.0 12-13 36.32256468490131 39.0 37.0 39.0 32.0 39.0 14-15 37.443220763407965 40.0 37.0 41.0 32.0 41.0 16-17 37.37308550703905 40.0 37.0 41.0 32.0 41.0 18-19 37.31356200635922 40.0 37.0 41.0 31.0 41.0 20-21 37.202681457575586 40.0 37.0 41.0 31.0 41.0 22-23 37.11434306043632 40.0 36.5 41.0 31.0 41.0 24-25 37.135306525181534 40.0 37.0 41.0 31.0 41.0 26-27 37.03961047608671 40.0 36.5 41.0 30.0 41.0 28-29 36.9204986174908 40.0 36.0 41.0 30.0 41.0 30-31 36.71852762461175 39.0 36.0 41.0 30.0 41.0 32-33 36.51070638548522 39.0 35.5 41.0 30.0 41.0 34-35 36.18667086200332 39.0 35.0 41.0 29.5 41.0 36-37 35.93896785823617 39.0 35.0 41.0 27.5 41.0 38-39 35.76761491652194 39.0 35.0 41.0 27.0 41.0 40-41 35.5985029467422 39.0 35.0 40.5 27.0 41.0 42-43 35.34283918049114 38.5 35.0 40.0 25.5 41.0 44-45 35.299992390084284 39.0 35.0 41.0 25.0 41.0 46-47 35.12380393991033 39.0 35.0 41.0 24.5 41.0 48-49 34.90511954609323 39.0 34.5 41.0 24.0 41.0 50-51 33.7795577897741 38.0 32.5 40.0 22.5 40.5 52-53 33.88371987010763 38.0 33.5 39.5 23.0 40.5 54-55 34.41447541861331 38.0 34.0 40.0 23.0 41.0 56-57 34.32588574601017 38.0 34.0 40.0 22.5 41.0 58-59 34.11319836110088 38.0 34.0 40.0 22.0 41.0 60-61 33.82774708692179 38.0 33.5 40.0 21.0 41.0 62-63 33.469446682533835 37.0 33.0 40.0 19.5 41.0 64-65 33.08222365690547 37.0 32.5 40.0 18.0 41.0 66-67 32.68755551470579 36.0 32.0 39.0 15.5 41.0 68-69 32.284607883032635 36.0 32.0 39.0 13.0 41.0 70-71 31.88466011472689 35.0 31.0 39.0 10.0 40.0 72-73 31.470695400522466 35.0 31.0 38.0 8.0 40.0 74-75 31.01703719296893 35.0 31.0 37.0 7.0 39.0 76-77 30.576529315100174 35.0 30.0 37.0 2.0 39.0 78-79 30.161003950485146 35.0 30.0 36.0 2.0 39.0 80-81 29.72817961674191 34.0 29.5 36.0 2.0 37.5 82-83 29.27255135519504 34.0 29.0 35.0 2.0 37.0 84-85 28.86434015730289 34.0 29.0 35.0 2.0 36.5 86-87 28.482847669994523 34.0 29.0 35.0 2.0 36.0 88-89 28.106889049158326 34.0 27.5 35.0 2.0 36.0 90-91 27.69577632382267 34.0 27.0 35.0 2.0 35.5 92-93 27.257002543144885 34.0 26.0 35.0 2.0 35.0 94-95 26.76893170372424 33.5 25.0 35.0 2.0 35.0 96-97 26.1944867148908 33.0 24.5 35.0 2.0 35.0 98-99 25.508115431550166 33.0 21.0 35.0 2.0 35.0 100-101 23.25204077926525 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2789405123993518 1101 2 0.19203279242812954 1101 3 0.2997638046709383 1101 4 0.22821387369326374 1101 5 0.009921809663417491 1101 6 0.06901205799827892 1101 7 0.2803131159618317 1101 8 0.2908195145453334 1101 9 0.3637268450425495 1101 10-11 0.3633206652799146 1101 12-13 0.3049084756922298 1101 14-15 0.5390224787082971 1101 16-17 0.4946944169533438 1101 18-19 0.47668016533384616 1101 20-21 0.47903821424758775 1101 22-23 0.503925222008256 1101 24-25 0.5378491487544395 1101 26-27 0.5329454154005973 1101 28-29 0.4667355887697582 1101 30-31 0.4581490050241186 1101 32-33 0.46303276913179303 1101 34-35 0.3778893655505371 1101 36-37 0.5697203949634471 1101 38-39 0.6748398415989669 1101 40-41 0.49723346459780515 1101 42-43 0.48546044620125883 1101 44-45 0.5569503131667517 1101 46-47 0.628211286242589 1101 48-49 0.6557082783840258 1101 50-51 0.5253894311776506 1101 52-53 0.47700314960746226 1101 54-55 0.551481491281244 1101 56-57 0.6028162806326236 1101 58-59 0.6614287676631534 1101 60-61 0.5805912445705701 1101 62-63 0.6393883751265861 1101 64-65 0.5969747203824483 1101 66-67 0.5135715910741183 1101 68-69 0.5446672328777709 1101 70-71 0.4581000209743742 1101 72-73 0.3736899310785553 1101 74-75 0.29065069526268417 1101 76-77 0.3044944303212773 1101 78-79 0.1287834991586898 1101 80-81 0.06739793156758722 1101 82-83 -0.010764818797834863 1101 84-85 -0.025838836522712683 1101 86-87 -0.05106595981301254 1101 88-89 -0.06857259514805847 1101 90-91 -0.07255143613492265 1101 92-93 -0.13177656289333584 1101 94-95 -0.24140051931657425 1101 96-97 -0.42458670663126696 1101 98-99 -0.3298873988485731 1101 100-101 -0.1499818431009423 1102 1 0.4161568410379637 1102 2 0.46558786577232425 1102 3 0.4819261627631377 1102 4 0.4298068722031232 1102 5 0.37007856311701914 1102 6 0.3632694318445431 1102 7 0.3694198433488296 1102 8 0.32056947524670676 1102 9 0.4973941804056494 1102 10-11 0.4677847909514128 1102 12-13 0.46471882422043365 1102 14-15 0.5104666065697572 1102 16-17 0.5092560019459569 1102 18-19 0.6670583091922282 1102 20-21 0.6460893193917414 1102 22-23 0.6283296201724866 1102 24-25 0.6653480095429245 1102 26-27 0.5403206079510596 1102 28-29 0.4803140989708652 1102 30-31 0.5543105738100493 1102 32-33 0.5703339610150167 1102 34-35 0.6639634887936054 1102 36-37 0.7197291329786495 1102 38-39 0.6735524748841542 1102 40-41 0.6146366803412633 1102 42-43 0.7550800149446246 1102 44-45 0.8654761349953546 1102 46-47 0.8212773794868866 1102 48-49 0.8903807226972944 1102 50-51 0.807292193929058 1102 52-53 0.8017987326209095 1102 54-55 0.6720899174204575 1102 56-57 0.6905672693810345 1102 58-59 0.6771576117295197 1102 60-61 0.6735896582650085 1102 62-63 0.6964825856754402 1102 64-65 0.7352205064537785 1102 66-67 0.7324924327341051 1102 68-69 0.4911994589086248 1102 70-71 0.5486392571628649 1102 72-73 0.5084574357655391 1102 74-75 0.5466376140115798 1102 76-77 0.5527680472595122 1102 78-79 0.4729044561117526 1102 80-81 0.5142691964347144 1102 82-83 0.548709651452782 1102 84-85 0.44968689965666897 1102 86-87 0.2953576212115294 1102 88-89 0.4563548850955641 1102 90-91 0.5214834548943976 1102 92-93 0.39640648512638776 1102 94-95 0.3595158631989861 1102 96-97 0.42464486731157436 1102 98-99 0.2380753832328928 1102 100-101 0.43287431852682445 1103 1 0.23272424804447667 1103 2 0.2737880884389945 1103 3 0.2939101089919731 1103 4 0.3318865594783418 1103 5 0.25902119795824063 1103 6 0.33154875816160256 1103 7 0.27049773503225794 1103 8 0.30127171849714074 1103 9 0.3763244170522313 1103 10-11 0.37075911197011635 1103 12-13 0.3837742897877163 1103 14-15 0.45917297301205195 1103 16-17 0.523688387195584 1103 18-19 0.5968061955325652 1103 20-21 0.46795675018786653 1103 22-23 0.5304607323585202 1103 24-25 0.3694600603990281 1103 26-27 0.42720228568334306 1103 28-29 0.44154230343273326 1103 30-31 0.32350567238362515 1103 32-33 0.3461468067916442 1103 34-35 0.43819008368770795 1103 36-37 0.36094886925892666 1103 38-39 0.24710127667734127 1103 40-41 0.38384647174649444 1103 42-43 0.39025642985792786 1103 44-45 0.4284806900286924 1103 46-47 0.3399834622936453 1103 48-49 0.36237414133081813 1103 50-51 0.47190879534677777 1103 52-53 0.39916598799739944 1103 54-55 0.44234242075956587 1103 56-57 0.41888713385945664 1103 58-59 0.3193597235110488 1103 60-61 0.3923099931184737 1103 62-63 0.4142781664805142 1103 64-65 0.24739179267448463 1103 66-67 0.17981470249295484 1103 68-69 0.12380140224641423 1103 70-71 0.22807776357391774 1103 72-73 0.2902343893819328 1103 74-75 0.13228634596909927 1103 76-77 0.11097925760898875 1103 78-79 0.27346954880563246 1103 80-81 0.19047131920392602 1103 82-83 0.172832585225418 1103 84-85 0.25791376106596076 1103 86-87 0.1461759991586895 1103 88-89 0.023048554631550644 1103 90-91 -1.8642758594467068E-4 1103 92-93 0.0804644497526894 1103 94-95 -0.030719293655298685 1103 96-97 -0.05580807691469403 1103 98-99 -0.26793194069651705 1103 100-101 0.015079210447630942 1104 1 0.18337388141595312 1104 2 0.2891498566608597 1104 3 0.2289931505652909 1104 4 0.27398584969460416 1104 5 0.2396801354690723 1104 6 0.2850131066494228 1104 7 0.18257730955954798 1104 8 0.20628734055409836 1104 9 0.18988366208237295 1104 10-11 0.20696082722558629 1104 12-13 0.2940375221574172 1104 14-15 0.40160855628125347 1104 16-17 0.3422993161045724 1104 18-19 0.3162735217235735 1104 20-21 0.29860910949233954 1104 22-23 0.4753727500316529 1104 24-25 0.41700790383582387 1104 26-27 0.4412317541548063 1104 28-29 0.43747271263502796 1104 30-31 0.36243672804850036 1104 32-33 0.45876844445268006 1104 34-35 0.4768516695181546 1104 36-37 0.5695464429892638 1104 38-39 0.5280088609233431 1104 40-41 0.3586801472412091 1104 42-43 0.5719990203839842 1104 44-45 0.7169473432550006 1104 46-47 0.6521084690374863 1104 48-49 0.6567277686673805 1104 50-51 0.7039230799928688 1104 52-53 0.6581855607641955 1104 54-55 0.711557740124384 1104 56-57 0.6185631453651723 1104 58-59 0.5224744805575412 1104 60-61 0.5012655348550581 1104 62-63 0.5918729849362165 1104 64-65 0.5169751129933786 1104 66-67 0.5118075014866363 1104 68-69 0.47823781086755446 1104 70-71 0.3629639322088458 1104 72-73 0.4326968533792659 1104 74-75 0.29924425207396155 1104 76-77 0.12411541512248547 1104 78-79 0.26265914217782793 1104 80-81 0.2283411540104936 1104 82-83 0.2883021603111473 1104 84-85 0.3442014662563082 1104 86-87 0.4045280969439631 1104 88-89 0.391045438738697 1104 90-91 0.31196072736346636 1104 92-93 0.4549710132882261 1104 94-95 0.43919994111473315 1104 96-97 0.43514106834440724 1104 98-99 0.5212768667172618 1104 100-101 0.7267452571538442 1105 1 0.11836648509475367 1105 2 0.13886895778445663 1105 3 0.2183556646195406 1105 4 0.22826460909823254 1105 5 0.17279437140178544 1105 6 0.11295333047500833 1105 7 0.20277838773468915 1105 8 0.1561651026953612 1105 9 0.22329685334677407 1105 10-11 0.20454383728012004 1105 12-13 0.1674445829139728 1105 14-15 0.29640454315650544 1105 16-17 0.23897978261884845 1105 18-19 0.2666711980873657 1105 20-21 0.2858261009270038 1105 22-23 0.318272747053264 1105 24-25 0.32977499665965126 1105 26-27 0.27729235683088405 1105 28-29 0.26993645080525397 1105 30-31 0.2299915737557825 1105 32-33 0.1762279198102661 1105 34-35 0.19025455433162364 1105 36-37 0.24118583261902415 1105 38-39 0.32817906318046397 1105 40-41 0.29453077381882053 1105 42-43 0.2378655564981358 1105 44-45 0.3185113363252867 1105 46-47 0.29154525655209795 1105 48-49 0.29920306669197316 1105 50-51 0.39498519668099874 1105 52-53 0.4336803448685558 1105 54-55 0.42195636584819596 1105 56-57 0.34026802842635107 1105 58-59 0.383712141979764 1105 60-61 0.4249298225372158 1105 62-63 0.2709268452946674 1105 64-65 0.3766223481839859 1105 66-67 0.250183053418489 1105 68-69 0.29052861039002664 1105 70-71 0.25542244579648 1105 72-73 0.2975766135200466 1105 74-75 0.22184094408171617 1105 76-77 0.19634151544936174 1105 78-79 0.006292349178021794 1105 80-81 0.0037727715462274602 1105 82-83 0.04113075677233269 1105 84-85 0.06374031928048396 1105 86-87 -0.06117670669363662 1105 88-89 -0.13567647075316458 1105 90-91 0.10713323166351074 1105 92-93 0.14568106012331938 1105 94-95 0.17315526038512985 1105 96-97 0.11292607490926088 1105 98-99 0.09843157268867486 1105 100-101 0.4379348300783441 1106 1 0.08683258094990265 1106 2 0.03951636814302262 1106 3 0.1620129440505238 1106 4 0.21361149194458307 1106 5 0.15662247845666855 1106 6 0.17972303099697484 1106 7 0.19285436612728546 1106 8 0.2011288857341782 1106 9 0.23360353912849519 1106 10-11 0.17910533284854324 1106 12-13 0.13221101420669612 1106 14-15 0.19021399718928222 1106 16-17 0.257646665782012 1106 18-19 0.22650631091636342 1106 20-21 0.1430428601327236 1106 22-23 0.12819731320218608 1106 24-25 0.09320735074233255 1106 26-27 -0.02903996794555752 1106 28-29 0.05434305896506686 1106 30-31 0.07679570910305955 1106 32-33 0.12199876287935041 1106 34-35 0.07192617797620926 1106 36-37 0.23738097253649215 1106 38-39 0.13743607093473287 1106 40-41 0.1661278843825258 1106 42-43 0.2727803208997699 1106 44-45 0.23301633818662992 1106 46-47 0.2900767270914173 1106 48-49 0.3996380563555846 1106 50-51 0.35890412335997013 1106 52-53 0.31124439285157024 1106 54-55 0.1877396541661014 1106 56-57 0.3191036798564184 1106 58-59 0.2254956045509502 1106 60-61 0.22440686466690352 1106 62-63 -0.004207953482392668 1106 64-65 -0.10598796602637606 1106 66-67 0.016544545818668155 1106 68-69 0.1700723143020184 1106 70-71 0.23409498229066017 1106 72-73 0.08178520658318078 1106 74-75 0.036234710962503414 1106 76-77 0.1056827786272656 1106 78-79 0.3386077199018871 1106 80-81 0.13937078703893135 1106 82-83 0.16244643837989514 1106 84-85 0.25464741653757983 1106 86-87 0.4114680027390101 1106 88-89 0.46218432265372655 1106 90-91 0.43498855985373197 1106 92-93 0.49004243173429174 1106 94-95 0.5463607485715478 1106 96-97 0.6780965560067429 1106 98-99 0.6643057499359486 1106 100-101 0.8371535615144303 1107 1 0.10304425407036533 1107 2 0.11244144117111787 1107 3 0.07882275379015269 1107 4 0.10286557578152156 1107 5 0.04653011348387537 1107 6 0.10816199480700561 1107 7 -0.07742281949400365 1107 8 0.06296403345243817 1107 9 0.047525429710397304 1107 10-11 0.03237365262873482 1107 12-13 0.1546437727124257 1107 14-15 0.12597899773350463 1107 16-17 0.222930006943578 1107 18-19 0.24909752371714688 1107 20-21 0.30295575592832336 1107 22-23 0.19279202934132655 1107 24-25 0.14931016076729975 1107 26-27 0.21317698751588665 1107 28-29 0.04948971820552828 1107 30-31 0.14609879457069042 1107 32-33 0.22142504862977574 1107 34-35 0.21534573136491275 1107 36-37 0.3369565810058859 1107 38-39 0.3896462800613065 1107 40-41 0.3305255484640881 1107 42-43 0.30714964277544965 1107 44-45 0.2718422675481946 1107 46-47 0.4107769498283318 1107 48-49 0.2992021492333947 1107 50-51 0.42284829131036616 1107 52-53 0.304924147130059 1107 54-55 0.2903866590581714 1107 56-57 0.21515557858687373 1107 58-59 0.1977660109882109 1107 60-61 0.14737037611458703 1107 62-63 0.17468369748695523 1107 64-65 0.1506304042042501 1107 66-67 0.13177909126964948 1107 68-69 0.28150962560320636 1107 70-71 0.2657409494924927 1107 72-73 0.1742414338522984 1107 74-75 0.10117550770661055 1107 76-77 0.07530515057192488 1107 78-79 0.12339908606411498 1107 80-81 -0.026266718317444315 1107 82-83 0.10630897126582539 1107 84-85 0.08713334165326359 1107 86-87 0.12680000478252396 1107 88-89 0.02700046419143831 1107 90-91 0.1791239736501815 1107 92-93 0.41350177550613765 1107 94-95 0.3416287903596036 1107 96-97 0.2433019846000235 1107 98-99 0.27450291353358836 1107 100-101 0.4192068399095845 1108 1 0.07423361829170005 1108 2 0.008422334690774136 1108 3 -0.036935332077593586 1108 4 -0.009401201893957989 1108 5 -0.007420574699864346 1108 6 0.08009985823172627 1108 7 -0.071898713059241 1108 8 -0.07623858700377184 1108 9 -0.01840934856063825 1108 10-11 0.04407211785087384 1108 12-13 0.09893173119350962 1108 14-15 -0.07984055825150449 1108 16-17 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56-57 -2.725268862745729 2316 58-59 -2.4140980853605782 2316 60-61 -2.3216424425830127 2316 62-63 -2.7833235481280596 2316 64-65 -2.9312717119689715 2316 66-67 -2.830939616287548 2316 68-69 -3.0965528770888575 2316 70-71 -2.8232848222839166 2316 72-73 -2.7965102743726113 2316 74-75 -2.920508531275015 2316 76-77 -2.976785687033793 2316 78-79 -2.8433069091066585 2316 80-81 -2.678117153921722 2316 82-83 -2.5299635426268168 2316 84-85 -2.598938431244548 2316 86-87 -2.697662331941494 2316 88-89 -2.8541860653594426 2316 90-91 -3.069260964878886 2316 92-93 -3.143448375628349 2316 94-95 -3.2272615526974846 2316 96-97 -3.21760518476885 2316 98-99 -3.4293441207582482 2316 100-101 -3.7028402756777687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15885.0 3 5449.0 4 5559.0 5 6008.0 6 6947.0 7 8160.0 8 9906.0 9 12249.0 10 14667.0 11 18116.0 12 21692.0 13 26592.0 14 28329.0 15 26906.0 16 31442.0 17 38065.0 18 33348.0 19 27426.0 20 27724.0 21 30085.0 22 32780.0 23 36940.0 24 41834.0 25 48261.0 26 55233.0 27 63339.0 28 73856.0 29 86537.0 30 102962.0 31 122980.0 32 149556.0 33 188100.0 34 245912.0 35 341187.0 36 499502.0 37 742875.0 38 700463.0 39 120446.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16477943310977 18.955529757245383 14.036686780934318 22.843004028710535 2 15.140965417361407 20.343620362094576 40.59101159052834 23.924402630015678 3 19.278240329040624 25.107746362540173 29.623671655757665 25.990341652661535 4 12.467475548537859 16.516732655439668 37.84597112323379 33.16982067278869 5 13.386127532848766 37.82729711295998 34.35654564539388 14.430029708797381 6 31.414846114583245 36.412615353813486 17.819144005803686 14.353394525799581 7 28.291032090345258 32.86695223167596 20.99199440767837 17.850021270300406 8 25.991809041102123 35.085192300656836 20.018954693635 18.90404396460604 9 26.556838083796514 16.921165918284863 20.61976642577548 35.90222957214314 10-11 24.758428461923888 26.821170444413518 27.532691350786028 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22.142331349267923 26.789711824326425 29.10834983273025 21.959606993675404 38-39 21.942326312865816 27.109224270317085 29.23096455331176 21.717484863505344 40-41 22.06294847697273 26.950971377253996 29.240093756060293 21.745986389712986 42-43 21.84146170733088 27.024460582375433 28.87817963741286 22.25589807288083 44-45 22.002942232253638 27.094751695159715 28.761474631314865 22.140831441271782 46-47 22.19645140573694 27.20020862693735 28.416346779476807 22.1869931878489 48-49 22.231243275332513 27.279217690963797 28.321347953894804 22.168191079808885 50-51 22.068517536282272 27.430810237598514 28.34753525571839 22.153136970400826 52-53 22.187793198769246 27.531840812201093 28.182237768731145 22.09812822029852 54-55 21.97348982017138 27.288000609714775 28.344611980845624 22.393897589268224 56-57 22.12561760580973 27.585865403910752 28.069078774687945 22.21943821559157 58-59 22.303413836195816 27.223688507423134 28.19258649731652 22.280311159064535 60-61 22.15044083246952 27.28248219174752 28.51182187145759 22.05525510432537 62-63 22.063010365042715 27.2982778212709 28.54196876735967 22.09674304632671 64-65 21.914585476290537 27.309616940127146 28.426746092394485 22.34905149118783 66-67 21.90282953942867 27.49609244974007 28.055211397568918 22.54586661326234 68-69 21.83847531452927 27.37766471228874 28.266503326548325 22.51735664663366 70-71 22.023848251672884 27.18730242137954 28.218262385897958 22.570586941049616 72-73 22.025008492100856 27.12020692915701 28.201410284679056 22.653374294063077 74-75 21.88360482259882 27.52450752792401 27.983857632131592 22.608030017345584 76-77 22.058932137543323 27.19027536443614 28.108231292373464 22.642561205647077 78-79 21.955063036907585 27.194465791726607 28.200281614532074 22.65018955683373 80-81 22.008562043542895 27.399991288293812 27.99452180116883 22.59692486699446 82-83 22.14740502809937 27.27061282443354 27.994829417955795 22.58715272951129 84-85 21.992025632356977 27.25508097237343 27.936474131087824 22.81641926418177 86-87 22.0023414669022 27.289148820180703 28.05024968696037 22.658260025956718 88-89 22.14178113693791 27.159331072789872 27.874437424470106 22.82445036580211 90-91 22.15331325611562 27.105435683859174 28.00831459499682 22.732936465028388 92-93 22.31968908926021 27.175440981397376 27.921743453947183 22.583126475395236 94-95 22.115514854050517 26.98771493402828 28.00505391918968 22.89171629273152 96-97 22.157596416644754 26.842661076799345 28.01603163618585 22.983710870370054 98-99 22.14009670831704 26.904353115708584 27.779755808395873 23.175794367578504 100-101 22.46698014725031 26.462690787508876 28.112709021880498 22.95762004336032 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4100.0 1 2442.5 2 1057.0 3 1373.0 4 1175.5 5 1202.5 6 2230.5 7 4338.0 8 6560.5 9 7202.5 10 6090.5 11 5031.0 12 5063.0 13 5394.0 14 5656.5 15 5873.5 16 6133.0 17 6528.0 18 6714.0 19 7008.0 20 7768.5 21 8779.5 22 10163.5 23 12113.5 24 14390.0 25 17507.0 26 22148.0 27 27826.5 28 32818.0 29 39191.0 30 47815.0 31 55781.5 32 64497.5 33 73811.5 34 82815.0 35 91939.0 36 103463.0 37 113283.5 38 117201.0 39 119199.0 40 121086.0 41 125265.0 42 129191.5 43 134113.0 44 140460.0 45 144498.0 46 145960.5 47 151142.5 48 168620.0 49 168981.0 50 153182.0 51 143974.0 52 135585.0 53 131289.0 54 126879.5 55 117857.0 56 107189.5 57 95968.5 58 86054.5 59 75820.5 60 64089.0 61 53264.0 62 42832.5 63 33301.5 64 25072.5 65 18769.5 66 14099.5 67 10847.5 68 7931.0 69 5379.0 70 3999.5 71 3039.5 72 2348.5 73 1999.5 74 1616.5 75 1224.0 76 896.5 77 557.5 78 366.5 79 270.0 80 179.0 81 118.0 82 92.0 83 70.0 84 50.0 85 39.0 86 32.0 87 19.0 88 13.5 89 14.0 90 15.5 91 10.0 92 9.5 93 10.0 94 5.5 95 4.5 96 5.0 97 3.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16316845264964663 2 0.09579104950373712 3 0.08882352926642637 4 0.10473517122680984 5 0.12635424997733086 6 0.040668575569551536 7 0.04457236350393134 8 0.047314898065426006 9 0.048327906326878986 10-11 0.04600539958110873 12-13 0.050563936757647164 14-15 0.04898265556903763 16-17 0.047327251824712015 18-19 0.0515275299819561 20-21 0.053924159283442435 22-23 0.06690796029304105 24-25 0.08358553532915727 26-27 0.08545095298134509 28-29 0.075086148940381 30-31 0.06307829491437733 32-33 0.05260230703983915 34-35 0.30209882958013773 36-37 0.47996825578013863 38-39 0.4573855838053087 40-41 0.4331598618454392 42-43 0.46319185066973434 44-45 0.4723336325413832 46-47 0.472111264874235 48-49 0.4876152327781801 50-51 0.47726278249650206 52-53 0.48377321364023035 54-55 0.4753232422885982 56-57 0.45426008270594764 58-59 0.4327521877890007 60-61 0.4023495861861252 62-63 0.45997987325537126 64-65 0.496831137205545 66-67 0.49937601161846357 68-69 0.5084683784529684 70-71 0.5058370277250478 72-73 0.49610226540767033 74-75 0.489530065467512 76-77 0.472098911114949 78-79 0.4468231196157684 80-81 0.45188816092303336 82-83 0.4642789814869034 84-85 0.46185764466684504 86-87 0.47208655735566296 88-89 0.48324200199093187 90-91 0.4875905252596081 92-93 0.4899130320053784 94-95 0.493693282346898 96-97 0.49573165262908997 98-99 0.4886158872803471 100-101 0.4754714874000303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4047351.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.53836494331304 #Duplication Level Percentage of deduplicated Percentage of total 1 70.26349253645301 33.40211550389655 2 14.64546885720353 13.924432865993344 3 5.993211866641836 8.547224786970418 4 3.058121266095124 5.815123391541461 5 1.7100826387942896 4.064726628311336 6 1.0378399939692275 2.960232983164497 7 0.7233941451434663 2.4072282408780246 8 0.47450092611584815 1.8045598553308169 9 0.32776721524014923 1.4023365745085685 >10 1.5811842104349951 13.05638623746608 >50 0.11486711602751704 3.769792703980828 >100 0.06501417806891298 5.633134570262478 >500 0.0037000880551486047 1.1573717924048605 >1k 0.0011465061020491214 1.0222290171476849 >5k 1.0422782745901089E-4 0.3562763679802444 >10k+ 1.0422782745901089E-4 0.6768284801627339 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15559 0.38442428146212176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11761 0.29058512592557456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8080 0.19963675006195414 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6301 0.15568207452232335 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.4707518572024022E-5 2.4707518572024022E-5 2 0.0 0.0 0.0 2.4707518572024022E-5 2.4707518572024022E-5 3 0.0 0.0 0.0 2.4707518572024022E-5 2.4707518572024022E-5 4 0.0 0.0 0.0 2.4707518572024022E-5 2.4707518572024022E-5 5 0.0 0.0 0.0 2.4707518572024022E-5 2.4707518572024022E-5 6 0.0 0.0 0.0 4.9415037144048044E-5 7.412255571607207E-5 7 0.0 0.0 0.0 4.9415037144048044E-5 7.412255571607207E-5 8 0.0 0.0 0.0 4.9415037144048044E-5 7.412255571607207E-5 9 0.0 0.0 0.0 1.4824511143214414E-4 7.412255571607207E-5 10-11 0.0 0.0 0.0 2.470751857202402E-4 9.883007428809609E-5 12-13 0.0 0.0 0.0 2.964902228642883E-4 2.3472142643422822E-4 14-15 0.0 0.0 0.0 3.0884398215030027E-4 2.594289450062522E-4 16-17 0.0 0.0 0.0 4.941503714404804E-4 2.7178270429226425E-4 18-19 0.0 0.0 0.0 7.412255571607206E-4 3.0884398215030027E-4 20-21 0.0 0.0 0.0 8.771169093068527E-4 3.3355150072232435E-4 22-23 4.9415037144048044E-5 0.0 0.0 0.001173607132171141 3.5825901929434833E-4 24-25 7.412255571607207E-5 0.0 0.0 0.0016059887071815616 3.706127785803603E-4 26-27 7.412255571607207E-5 0.0 0.0 0.0025325206536324623 3.9532029715238435E-4 28-29 7.412255571607207E-5 0.0 0.0 0.0053738852894152245 3.9532029715238435E-4 30-31 7.412255571607207E-5 0.0 0.0 0.014305653253201909 3.9532029715238435E-4 32-33 7.412255571607207E-5 0.0 0.0 0.030328479047159487 3.9532029715238435E-4 34-35 8.647631500208409E-5 0.0 0.0 0.05539425663847786 4.076740564383964E-4 36-37 9.883007428809609E-5 0.0 0.0 0.09952188480811276 4.447353342964324E-4 38-39 9.883007428809609E-5 0.0 0.0 0.16279783987106627 4.6944285286845645E-4 40-41 1.235375928601201E-4 0.0 0.0 0.23362194185777313 4.6944285286845645E-4 42-43 1.235375928601201E-4 0.0 0.0 0.305496113383791 4.6944285286845645E-4 44-45 1.3589135214613212E-4 0.0 0.0 0.3964815505252695 4.8179661215446844E-4 46-47 1.4824511143214414E-4 0.0 0.0 0.5087401611572606 5.312116492985164E-4 48-49 1.4824511143214414E-4 0.0 0.0 0.629572280733744 5.682729271565525E-4 50-51 1.4824511143214414E-4 0.0 0.0 0.7599538562383149 5.682729271565525E-4 52-53 1.4824511143214414E-4 0.0 0.0 0.8873211144770987 5.682729271565525E-4 54-55 1.4824511143214414E-4 0.0 0.0 1.0320701120313014 5.682729271565525E-4 56-57 1.4824511143214414E-4 0.0 0.0 1.2041579788854486 5.806266864425646E-4 58-59 1.4824511143214414E-4 0.0 0.0 1.3957771391707812 6.053342050145885E-4 60-61 1.4824511143214414E-4 0.0 0.0 1.6071128992765886 6.176879643006005E-4 62-63 1.4824511143214414E-4 0.0 0.0 1.8189428097538365 6.176879643006005E-4 64-65 1.4824511143214414E-4 0.0 0.0 2.037530226560533 6.300417235866125E-4 66-67 1.4824511143214414E-4 0.0 0.0 2.283456512667174 6.423954828726246E-4 68-69 1.7295263000416817E-4 2.4707518572024022E-5 0.0 2.5696807615647863 6.423954828726246E-4 70-71 1.7295263000416817E-4 2.4707518572024022E-5 0.0 2.879982487310836 6.547492421586366E-4 72-73 1.7295263000416817E-4 2.4707518572024022E-5 0.0 3.196473446459178 8.400556314488168E-4 74-75 1.7295263000416817E-4 2.4707518572024022E-5 0.0 3.5118402135124924 8.647631500208407E-4 76-77 1.7295263000416817E-4 2.4707518572024022E-5 0.0 3.8614392475473465 8.771169093068527E-4 78-79 1.7295263000416817E-4 2.4707518572024022E-5 0.0 4.260280366096245 9.141781871648888E-4 80-81 1.7295263000416817E-4 2.4707518572024022E-5 0.0 4.6965904365596165 9.388857057369129E-4 82-83 1.7295263000416817E-4 2.4707518572024022E-5 0.0 5.13450649573017 9.883007428809609E-4 84-85 1.7295263000416817E-4 2.4707518572024022E-5 0.0 5.575585117277942 9.883007428809609E-4 86-87 1.7295263000416817E-4 2.4707518572024022E-5 0.0 6.033217776268971 0.0010006545021669728 88-89 1.7295263000416817E-4 2.4707518572024022E-5 0.0 6.530604832642387 0.001025362020738997 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18330 0.0 25.577147 1 GTATCAA 26020 0.0 24.868156 1 TACATGG 18705 0.0 24.317383 2 ACATGGG 18825 0.0 23.432468 3 GTATAGG 1135 0.0 22.135954 1 GGTATCA 19120 0.0 21.396421 1 GAGTACT 13755 0.0 21.240368 12-13 TCAACGC 30115 0.0 21.239986 4 CAACGCA 30235 0.0 21.124607 5 TACCTGG 3075 0.0 21.10954 2 ATCAACG 30315 0.0 21.037338 3 CATGGGG 7410 0.0 20.972923 4 TATCAAC 30620 0.0 20.905159 2 AACGCAG 30805 0.0 20.810825 6 AGTACTT 14425 0.0 19.90914 12-13 GTACTTT 14645 0.0 19.836637 14-15 CATGGGA 11020 0.0 18.96096 4 ACGCAGA 33860 0.0 18.864662 7 CGCAGAG 33955 0.0 18.728212 8 AGAGTAC 25820 0.0 17.935663 10-11 >>END_MODULE