FastQCFastQC Report
Thu 26 May 2016
SRR938729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4047351
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153800.3800016356377295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128330.3170715858347843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86820.2145106762423126No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72770.17979661264861882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG181800.023.3202781
GTATCAA274000.022.7402271
TACATGG184800.022.4415972
GGTATCA209800.022.4103811
ACATGGG183250.021.8545953
GAGTACT148550.020.97064212-13
GTACTTT155550.019.82864414-15
TCAACGC317550.019.4256484
ATCAACG319200.019.3549653
CAACGCA319500.019.2773845
TATCAAC323900.019.1034132
AGTACTT153950.019.0792212-13
GTACCGT2752.8765498E-818.9803526
AACGCAG325800.018.9628756
CATGGGA112000.018.1330174
AGAGTAC270650.017.53230310-11
CATGGGG72700.017.29644
ACGCAGA357100.017.2210437
TACCTGG31050.017.1159382
CGCAGAG357950.017.1006138
Produced by FastQC (version 0.11.5)