##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938729_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4047351 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03417494553845 33.0 31.0 34.0 30.0 34.0 2 32.26260880264647 34.0 31.0 34.0 30.0 34.0 3 32.3515464806487 34.0 31.0 34.0 30.0 34.0 4 35.96808430995977 37.0 35.0 37.0 35.0 37.0 5 35.880778563559225 37.0 35.0 37.0 35.0 37.0 6 35.91002213546589 37.0 35.0 37.0 35.0 37.0 7 35.84568523955545 37.0 35.0 37.0 35.0 37.0 8 35.81193575748681 37.0 35.0 37.0 33.0 37.0 9 37.47556661134653 39.0 37.0 39.0 34.0 39.0 10-11 37.404007213607116 39.0 37.0 39.0 34.0 39.0 12-13 37.33701796557798 39.0 37.0 39.0 34.0 39.0 14-15 38.57693649500624 40.0 38.0 41.0 34.0 41.0 16-17 38.51038568189416 40.0 38.0 41.0 34.0 41.0 18-19 38.53873879483149 40.0 38.0 41.0 33.5 41.0 20-21 38.61242909251014 40.0 38.0 41.0 34.0 41.0 22-23 38.57008497656862 40.0 38.0 41.0 34.0 41.0 24-25 38.507138866878606 40.0 38.0 41.0 34.0 41.0 26-27 38.3558168046211 40.0 38.0 41.0 34.0 41.0 28-29 38.204783202642915 40.0 38.0 41.0 33.0 41.0 30-31 37.99584654259045 40.0 37.0 41.0 33.0 41.0 32-33 37.78746061312695 40.0 37.0 41.0 33.0 41.0 34-35 37.754982085813666 40.0 37.0 41.0 33.0 41.0 36-37 37.67892122526561 40.0 37.0 41.0 33.0 41.0 38-39 37.58264306703323 40.0 37.0 41.0 32.0 41.0 40-41 37.44169013263243 40.0 37.0 41.0 32.0 41.0 42-43 37.2786200159067 40.0 36.5 41.0 31.0 41.0 44-45 37.04581564583847 40.0 36.0 41.0 31.0 41.0 46-47 36.77821839519231 40.0 36.0 41.0 30.5 41.0 48-49 36.521028939669435 39.5 36.0 41.0 30.0 41.0 50-51 36.30111374081467 39.0 35.0 41.0 30.0 41.0 52-53 36.103072355226914 39.0 35.0 41.0 29.0 41.0 54-55 35.87893983002709 39.0 35.0 40.5 28.5 41.0 56-57 35.633648650685345 38.5 35.0 40.0 28.0 41.0 58-59 35.346033492029726 38.0 34.5 40.0 28.0 41.0 60-61 35.01328918593914 38.0 34.0 40.0 27.0 41.0 62-63 34.65305714774924 37.5 34.0 40.0 26.0 41.0 64-65 34.30287044538514 37.0 34.0 40.0 26.0 41.0 66-67 33.91712579413053 36.5 33.0 39.0 26.0 41.0 68-69 33.48129418476431 36.0 33.0 39.0 25.5 40.0 70-71 33.01187492757609 35.0 32.0 38.5 24.5 40.0 72-73 32.65711659305062 35.0 32.0 38.0 23.5 40.0 74-75 32.23423370001762 35.0 32.0 37.0 22.5 39.0 76-77 30.17379021488376 33.0 29.5 35.5 20.0 37.5 78-79 31.233388949957636 34.5 31.0 36.0 20.0 38.5 80-81 31.28025009444449 35.0 31.0 36.0 20.5 37.5 82-83 30.973725407062545 34.5 31.0 35.5 20.0 37.0 84-85 30.58304703496188 34.0 31.0 35.0 18.5 37.0 86-87 30.05850987473041 34.0 30.0 35.0 13.0 36.0 88-89 29.545498895450383 34.0 30.0 35.0 7.0 36.0 90-91 29.130139071209786 34.0 29.0 35.0 3.5 35.5 92-93 28.854469997783735 34.0 29.0 35.0 2.0 35.0 94-95 28.476323155565208 34.0 29.0 35.0 2.0 35.0 96-97 27.970032744874366 34.0 29.0 35.0 2.0 35.0 98-99 27.25463914545588 33.0 27.0 35.0 2.0 35.0 100-101 24.091775707123006 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06288486950720085 1101 2 0.06535515128908287 1101 3 0.11177103723598236 1101 4 0.12917569295036913 1101 5 0.02074755804417805 1101 6 0.10836051697140903 1101 7 0.1267988318114348 1101 8 0.047944600594519216 1101 9 0.19690744019658268 1101 10-11 0.17418510828754563 1101 12-13 0.15674735449294275 1101 14-15 0.22484135956431572 1101 16-17 0.19365734067020668 1101 18-19 0.20172334332563224 1101 20-21 0.21187952737874838 1101 22-23 0.16301210582794567 1101 24-25 0.18071722637181864 1101 26-27 0.18374227158093248 1101 28-29 0.1647334258616624 1101 30-31 0.07750916916815953 1101 32-33 0.1344454023861843 1101 34-35 0.16838908702751354 1101 36-37 0.08017592964349518 1101 38-39 0.21441885612420464 1101 40-41 0.21676128340410372 1101 42-43 0.1311783045920123 1101 44-45 0.13197798886192658 1101 46-47 0.21866628272265132 1101 48-49 -0.01523587143117311 1101 50-51 0.048996675812780666 1101 52-53 0.14476158241208736 1101 54-55 0.03223747624351603 1101 56-57 -0.04839333304887106 1101 58-59 0.04941642371294819 1101 60-61 0.08997391822975231 1101 62-63 0.023115600654037394 1101 64-65 -0.003167915587425796 1101 66-67 -0.07427043772394626 1101 68-69 -0.027901551130341318 1101 70-71 -0.08249592003379291 1101 72-73 -0.0017287511472048323 1101 74-75 0.018990612351814207 1101 76-77 -0.07002805933664646 1101 78-79 -0.07491790421815026 1101 80-81 0.10514038122820324 1101 82-83 0.08336108097304873 1101 84-85 0.12149482639417286 1101 86-87 0.20157018088694656 1101 88-89 0.289914153167782 1101 90-91 0.32905457564295304 1101 92-93 0.3139884584037951 1101 94-95 0.23928476678381827 1101 96-97 0.17269576338615877 1101 98-99 0.22815842350792437 1101 100-101 0.6795379940051518 1102 1 0.12244633525764215 1102 2 0.1350344436932005 1102 3 0.15901337605453847 1102 4 0.13288181663860854 1102 5 0.10371600605215292 1102 6 0.1626216681020125 1102 7 0.14203817393313045 1102 8 0.1487360352625018 1102 9 0.15524595564946964 1102 10-11 0.3024373424114799 1102 12-13 0.2577127801605812 1102 14-15 0.2530124192579706 1102 16-17 0.3369099838076224 1102 18-19 0.2548640026758662 1102 20-21 0.29939068549720105 1102 22-23 0.20013745140586536 1102 24-25 0.13886851237943887 1102 26-27 0.17296422152640645 1102 28-29 0.22325383050549163 1102 30-31 0.195764068931652 1102 32-33 0.30061879537992553 1102 34-35 0.19880013659712148 1102 36-37 0.2735293901154918 1102 38-39 0.2952621649842584 1102 40-41 0.3212132284234528 1102 42-43 0.2569059703405614 1102 44-45 0.1924712424474606 1102 46-47 0.2652409615316742 1102 48-49 0.35414586687355154 1102 50-51 0.34343637478911404 1102 52-53 0.28900589090621054 1102 54-55 0.2689629053678928 1102 56-57 0.1702167096552074 1102 58-59 0.1514643888132028 1102 60-61 0.12095409274387947 1102 62-63 0.11085360739159711 1102 64-65 0.2129330089369006 1102 66-67 0.20035848832461767 1102 68-69 0.21755535410700588 1102 70-71 0.15127377768344985 1102 72-73 0.1924973889425985 1102 74-75 0.1270827132117205 1102 76-77 0.08038135802557633 1102 78-79 0.10640975865832658 1102 80-81 0.14870756085846182 1102 82-83 0.31429557163074406 1102 84-85 0.369522105669855 1102 86-87 0.35853407127954284 1102 88-89 0.41664935117518453 1102 90-91 0.5165734716809602 1102 92-93 0.5392245194424525 1102 94-95 0.4852231283245487 1102 96-97 0.6184427540598811 1102 98-99 0.6315483182337509 1102 100-101 1.1500771635205957 1103 1 0.09460571900706327 1103 2 0.059370001904540004 1103 3 -0.0036141673065586133 1103 4 0.07624631666198667 1103 5 0.020827737754402165 1103 6 0.11610141614167446 1103 7 0.09680360862503079 1103 8 0.11386930691149644 1103 9 0.07649026984302765 1103 10-11 0.11158177115801493 1103 12-13 0.16632860554125983 1103 14-15 0.20870104289227243 1103 16-17 0.13451977311752472 1103 18-19 0.11756172666681408 1103 20-21 0.16812489549430865 1103 22-23 0.11098454297900417 1103 24-25 0.10635535207399727 1103 26-27 0.17232275166063715 1103 28-29 0.2463919101600638 1103 30-31 0.1663960370070683 1103 32-33 0.179542643838829 1103 34-35 0.1270365777326532 1103 36-37 0.0031301501053988545 1103 38-39 0.15919881409362802 1103 40-41 0.17203949578239275 1103 42-43 0.20091452744572535 1103 44-45 0.19290912077190114 1103 46-47 0.28430443149811424 1103 48-49 0.35190003561318406 1103 50-51 0.33404650548377646 1103 52-53 0.2760153834975654 1103 54-55 0.2526220171082798 1103 56-57 0.304583837012423 1103 58-59 -0.01495198866020786 1103 60-61 0.21758236256968644 1103 62-63 0.2711333640169826 1103 64-65 0.3584897816551518 1103 66-67 0.20487554389138296 1103 68-69 0.23595189727978294 1103 70-71 0.1556526567507106 1103 72-73 0.2564703612871142 1103 74-75 0.49848309721845396 1103 76-77 0.37005427131768087 1103 78-79 0.2531501986715803 1103 80-81 0.4092737510429849 1103 82-83 0.3410072354137803 1103 84-85 0.22712540969163086 1103 86-87 0.28349487335994183 1103 88-89 0.5172348157995721 1103 90-91 0.5754153877999677 1103 92-93 0.4809948152896446 1103 94-95 0.48876572974278076 1103 96-97 0.5495498550926463 1103 98-99 0.764118959451924 1103 100-101 1.264865677456939 1104 1 -0.01041274256343172 1104 2 0.0034974619880756563 1104 3 0.019851761843789006 1104 4 -2.5850719825371016E-4 1104 5 -0.0022652686184159165 1104 6 0.03729052868957439 1104 7 -0.004944490557285519 1104 8 0.023289203761429178 1104 9 0.03385364901557608 1104 10-11 0.0218981970812564 1104 12-13 0.01832407367766109 1104 14-15 0.08792735117446426 1104 16-17 0.06748548409859012 1104 18-19 0.11904622434267509 1104 20-21 0.09239497487764226 1104 22-23 0.03194147797023561 1104 24-25 0.06906222246629312 1104 26-27 0.12867545331578611 1104 28-29 0.0957411409831721 1104 30-31 0.05612466469856514 1104 32-33 0.0418626778372726 1104 34-35 0.05649009442284836 1104 36-37 -0.00687506513445868 1104 38-39 0.019548982902705347 1104 40-41 0.04886802418557323 1104 42-43 0.1504518287152692 1104 44-45 0.11953779474747961 1104 46-47 0.050203104404147325 1104 48-49 -0.04147235049629927 1104 50-51 0.04989581817952171 1104 52-53 0.059975066191398696 1104 54-55 -0.014653535910646553 1104 56-57 -0.026326255470536353 1104 58-59 -0.10420868920227377 1104 60-61 -0.0635539918167467 1104 62-63 -0.1881169665497211 1104 64-65 0.02927241784058765 1104 66-67 -0.0919447688526489 1104 68-69 -0.1254007144574416 1104 70-71 -0.17281261457900143 1104 72-73 -0.28405404007244783 1104 74-75 -0.16719078086607908 1104 76-77 -0.15556116957831634 1104 78-79 -0.09984638728664308 1104 80-81 -0.22346214864268177 1104 82-83 -0.1960153832045286 1104 84-85 -0.06049601500589219 1104 86-87 0.08485736660426824 1104 88-89 0.18426919946896092 1104 90-91 0.18012793911234226 1104 92-93 0.19806795831878077 1104 94-95 0.21802852577389586 1104 96-97 0.25468473671805825 1104 98-99 0.30895639256954865 1104 100-101 0.8921968470809567 1105 1 -0.04436322195921605 1105 2 -0.04078199237190461 1105 3 -0.061953039808216204 1105 4 -0.09108781350771267 1105 5 -0.05961492846209637 1105 6 -0.059416929433098176 1105 7 -0.0022301895965100016 1105 8 -0.015478626212534152 1105 9 0.03142266686850803 1105 10-11 0.07799557039444238 1105 12-13 -0.02764710300754558 1105 14-15 0.010187392673294937 1105 16-17 -0.09831574473316351 1105 18-19 -0.14716065423604618 1105 20-21 -0.049509342042313165 1105 22-23 -0.08699177023094506 1105 24-25 -0.06304777050968369 1105 26-27 -0.0698549001439801 1105 28-29 -0.07585105986914442 1105 30-31 -0.21172414134159823 1105 32-33 -0.11568004956858147 1105 34-35 -0.07899592826748147 1105 36-37 -0.12630806141395823 1105 38-39 -0.061070390743061864 1105 40-41 -0.0945599535162458 1105 42-43 -0.13050791841990161 1105 44-45 -0.13175277643218664 1105 46-47 -0.028242576069814618 1105 48-49 -0.08827443437256477 1105 50-51 0.0014660782669366768 1105 52-53 -0.02670826701783824 1105 54-55 0.033142614775748314 1105 56-57 -0.04475119273831751 1105 58-59 -0.06017904683177733 1105 60-61 -0.12411341927198549 1105 62-63 -0.13692576792374922 1105 64-65 -0.11750084396188498 1105 66-67 -0.11787430400130461 1105 68-69 -0.1317070090246233 1105 70-71 -0.1902393277084613 1105 72-73 -0.2569083002790009 1105 74-75 -0.17984329896597728 1105 76-77 -0.17135104331963902 1105 78-79 -0.3174643375128774 1105 80-81 -0.18078841575908555 1105 82-83 -0.2155335426622642 1105 84-85 -0.1783241585044486 1105 86-87 -0.09518044646736712 1105 88-89 0.09759728339983198 1105 90-91 0.13932382497072737 1105 92-93 0.04712315895019614 1105 94-95 0.039579246418817604 1105 96-97 0.1041719582080951 1105 98-99 0.15778536008778232 1105 100-101 0.500060805651632 1106 1 -0.13649766628925164 1106 2 -0.1715521522888963 1106 3 -0.14901235721058015 1106 4 -0.10750673028592672 1106 5 -0.0888469071081488 1106 6 -0.15780617562922572 1106 7 -0.08968256696575594 1106 8 -0.06417697397456834 1106 9 -0.14484740196154178 1106 10-11 -0.12383998004792574 1106 12-13 -0.03447614865398663 1106 14-15 -3.259713655836549E-4 1106 16-17 -0.12025730255754041 1106 18-19 -0.2322640775210374 1106 20-21 -0.15382740775164194 1106 22-23 -0.16594410205672006 1106 24-25 -0.21727554133493499 1106 26-27 -0.07922645963167696 1106 28-29 -0.17143170943786146 1106 30-31 -0.12484303752624015 1106 32-33 -0.15266803203236634 1106 34-35 -0.10986623431961817 1106 36-37 -0.1018908198434616 1106 38-39 -0.08015137297714858 1106 40-41 -0.03905260913631281 1106 42-43 -0.05409711035989062 1106 44-45 -0.06264303250531356 1106 46-47 -0.02745101072087408 1106 48-49 -0.03385345031674092 1106 50-51 -0.08730136686308043 1106 52-53 -0.00673770196195278 1106 54-55 -0.011329348266713168 1106 56-57 -0.1228750789033981 1106 58-59 -0.03418709020500188 1106 60-61 -0.006788979456700872 1106 62-63 -0.029305800477423816 1106 64-65 -0.004425313083409321 1106 66-67 -0.13680801250723817 1106 68-69 -0.11954442674934285 1106 70-71 -0.04714916351434084 1106 72-73 -0.05863231871816055 1106 74-75 -0.027329843840156798 1106 76-77 -0.03898042638380517 1106 78-79 -0.06998086187269692 1106 80-81 -0.07648544870087548 1106 82-83 0.12577047585610757 1106 84-85 0.00826125926780108 1106 86-87 0.02255140940932776 1106 88-89 0.23400518063791864 1106 90-91 0.28829850280016345 1106 92-93 0.31078917084694524 1106 94-95 0.40245032670997816 1106 96-97 0.44798656063338527 1106 98-99 0.3059560966247368 1106 100-101 0.6905624669982906 1107 1 -0.0888972728837949 1107 2 -0.08949151960428026 1107 3 -0.14823231224154654 1107 4 -0.10743668067879497 1107 5 -0.020529602857273233 1107 6 -0.05535228212129795 1107 7 -0.03892902195309489 1107 8 -0.060355256169437155 1107 9 -0.048809819581244085 1107 10-11 -0.07611496207894675 1107 12-13 -0.12057128325219679 1107 14-15 -0.17706201880761085 1107 16-17 -0.04773506073477307 1107 18-19 -0.04273539677270577 1107 20-21 -0.12130934439100827 1107 22-23 -0.17833960373202729 1107 24-25 -0.16522687530498104 1107 26-27 -0.218888522676572 1107 28-29 -0.18244644326652093 1107 30-31 -0.28255130119810445 1107 32-33 -0.16529838068451852 1107 34-35 -0.31513514759097205 1107 36-37 -0.12254932537339158 1107 38-39 -0.1256304522299061 1107 40-41 -0.2181688110772555 1107 42-43 -0.3315067616881251 1107 44-45 -0.2794959628954885 1107 46-47 -0.3521712658068594 1107 48-49 -0.539253766355074 1107 50-51 -0.3693771309982665 1107 52-53 -0.38541395805007994 1107 54-55 -0.34959868661599813 1107 56-57 -0.3956441429376909 1107 58-59 -0.3391897598120792 1107 60-61 -0.3779844216603223 1107 62-63 -0.46931162139754434 1107 64-65 -0.43767195977255824 1107 66-67 -0.45075723653164346 1107 68-69 -0.36216948399022897 1107 70-71 -0.34909220356188797 1107 72-73 -0.3960843197222701 1107 74-75 -0.36761303178735716 1107 76-77 -0.36455543376260735 1107 78-79 -0.3559934997288501 1107 80-81 -0.36508413051803146 1107 82-83 -0.2245447596331438 1107 84-85 -0.31669957301223306 1107 86-87 -0.3516607010462174 1107 88-89 -0.2076959265748748 1107 90-91 0.01770771801903237 1107 92-93 -0.0018531346541585947 1107 94-95 -0.009993731661509742 1107 96-97 0.10769096232271735 1107 98-99 -0.06306725618602371 1107 100-101 0.39456202990835365 1108 1 -0.20699707750423357 1108 2 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sequence quality scores pass #Quality Count 2 9.0 3 15.0 4 53.0 5 113.0 6 257.0 7 598.0 8 1353.0 9 2375.0 10 3980.0 11 6123.0 12 8414.0 13 11715.0 14 14354.0 15 18942.0 16 24123.0 17 31869.0 18 30545.0 19 22089.0 20 20233.0 21 21787.0 22 23031.0 23 26052.0 24 30749.0 25 36326.0 26 44655.0 27 54937.0 28 67252.0 29 81871.0 30 99660.0 31 121637.0 32 149545.0 33 189171.0 34 249305.0 35 348082.0 36 526384.0 37 860161.0 38 822413.0 39 97164.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.58585607023321 19.924447501580726 14.700638914472975 22.789057513713086 2 15.627678449435198 21.097997183837034 39.57363717651373 23.700687190214044 3 19.38796511594868 25.163594657345012 29.88643683238741 25.5620033943189 4 12.939352183687552 17.19364097653008 37.30721649790196 32.55979034188041 5 13.33924337177576 38.04434060697972 34.366453514903945 14.249962506340566 6 31.465197854102595 36.900382497095016 17.352164415688186 14.282255233114203 7 28.205954956711192 32.975692002003285 20.834689158414974 17.983663882870548 8 26.02410811417147 35.496575414388325 19.72341909560105 18.755897375839158 9 26.27879321561189 16.880201395925383 20.476232478972047 36.364772909490675 10-11 24.79095586224175 27.088396830420685 27.283944486158973 20.836702821178594 12-13 25.779429557752714 24.296311340429828 27.835675729631554 22.08858337218591 14-15 22.55432519984118 25.76092588241311 26.43351594714453 25.25123297060118 16-17 21.797767855874365 29.054293203982084 26.95898190251242 22.188957037631127 18-19 21.6080020843291 27.790906410139684 29.277918082357928 21.323173423173287 20-21 22.62274756995378 26.660017873418933 29.30694669179916 21.41028786482813 22-23 22.142297517561488 26.705961504203614 29.136748950115766 22.01499202811913 24-25 22.047408292485628 26.896221750967484 29.338498193015628 21.717871763531257 26-27 21.96339551441803 26.991728282154316 29.358415287254324 21.686460916173328 28-29 21.660377367814156 27.25873046345622 29.37827729791659 21.702614870813033 30-31 21.885364031931008 26.972246785613603 29.27571638832412 21.866672794131272 32-33 21.66786374594148 27.15111686631577 29.206448860007445 21.974570527735303 34-35 22.186434714195773 27.049919990827853 29.08611388680506 21.677531408171312 36-37 22.145857975846226 26.896125143623145 29.11337366087133 21.8446432196593 38-39 21.944969607533288 27.18247168011197 29.230760106956176 21.641798605398563 40-41 22.036201085182554 26.984891011032452 29.264767691912724 21.71414021187227 42-43 21.823794212854235 27.090878138056055 28.87077051332387 22.21455713576584 44-45 21.997586770451765 27.127212924948118 28.74611760238096 22.12908270221916 46-47 22.204729000845504 27.277293749237142 28.42149373332523 22.096483516592123 48-49 22.224134310104144 27.375542021322595 28.28375356714887 22.116570101424387 50-51 22.046907915185653 27.50039902810097 28.30653462254412 22.14615843416926 52-53 22.18658574460184 27.57539437523457 28.11210344741536 22.125916432748234 54-55 21.979302017541844 27.345009118309726 28.224077921583774 22.451610942564656 56-57 22.120246057235953 27.610071377550405 28.021043887718168 22.248638677495478 58-59 22.26764441256614 27.343118122251507 28.08261680243291 22.30662066274944 60-61 22.147364878754576 27.44212972113948 28.329517647198347 22.080987752907596 62-63 22.01630152660345 27.418575754857933 28.366121445854336 22.19900127268428 64-65 21.888353641678222 27.408729808706976 28.247871261968633 22.455045287646165 66-67 21.90881728124069 27.564066135909798 27.938760191357108 22.588356391492407 68-69 21.83195342997578 27.47086377679872 28.10912492932059 22.588057863904908 70-71 21.94951767583052 27.33459376894792 28.048419209157366 22.667469346064195 72-73 21.905438890770778 27.29203619849131 28.035917813898525 22.76660709683939 74-75 21.770461716811813 27.665095021410302 27.754017380751016 22.810425881026873 76-77 21.918115678487 27.350545092914786 27.960690045004068 22.77064918359415 78-79 21.788087564474786 27.341659613167963 28.03596116478938 22.83429165756787 80-81 21.824122181689525 27.531190474160926 27.785430934491824 22.85925640965773 82-83 21.887587708602492 27.445852855361448 27.803753615636502 22.862805820399565 84-85 21.72285032852352 27.380970911591312 27.74139183876071 23.154786921124458 86-87 21.6795514241077 27.46495454443661 27.85116939025566 23.004324641200036 88-89 21.766908565689473 27.347856033620367 27.671531130457026 23.213704270233134 90-91 21.75172677293419 27.271899465094357 27.79494887324907 23.181424888722383 92-93 21.82000029154872 27.379846719496282 27.706010672165572 23.094142316789426 94-95 21.615696490827766 27.19316006854361 27.78460872118686 23.40653471944177 96-97 21.570296419843054 27.125560289379436 27.79498588026908 23.50915741050843 98-99 21.45234099964137 27.2261433832554 27.57228417715678 23.749231439946445 100-101 21.556948958398706 26.867072234853335 27.976780224770625 23.599198581977333 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2559.0 1 1628.0 2 950.0 3 1240.0 4 1067.5 5 1032.0 6 1987.0 7 4058.5 8 6032.5 9 6581.0 10 5509.0 11 4253.5 12 3994.0 13 4231.0 14 4469.0 15 4619.0 16 4771.0 17 5046.5 18 5469.0 19 6001.0 20 6880.5 21 8092.5 22 9690.0 23 11795.5 24 14069.5 25 17292.0 26 22270.0 27 28153.0 28 33428.0 29 39615.0 30 48032.5 31 55920.5 32 64900.5 33 74573.0 34 83507.5 35 92344.0 36 103746.5 37 113870.5 38 117690.0 39 119422.5 40 121429.5 41 125639.0 42 129368.0 43 134900.0 44 141788.5 45 145424.0 46 146981.5 47 152778.5 48 171158.5 49 171365.5 50 154492.0 51 145394.5 52 136912.0 53 132149.5 54 127999.0 55 118689.0 56 106829.5 57 95841.5 58 86333.5 59 75945.0 60 64672.5 61 53384.0 62 42511.5 63 32804.5 64 24290.5 65 17984.5 66 13302.5 67 10194.0 68 7363.0 69 4936.0 70 3656.0 71 2802.5 72 2139.5 73 1880.5 74 1521.5 75 1104.0 76 769.5 77 426.5 78 255.5 79 165.5 80 94.5 81 56.5 82 37.5 83 25.0 84 15.5 85 9.0 86 5.5 87 4.0 88 2.0 89 3.5 90 3.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.394875561818088 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.964902228642883E-4 16-17 0.0018901251707598378 18-19 1.3589135214613212E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.3589135214613212E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007782868350187567 36-37 0.01819708742829569 38-39 0.01136545854313105 40-41 0.007165180385886967 42-43 0.0034590526000833634 44-45 0.0019148326893318618 46-47 0.0017048187814696575 48-49 2.4707518572024022E-5 50-51 4.200278157244084E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.5825901929434833E-4 60-61 5.682729271565526E-4 62-63 0.0 64-65 0.0 66-67 0.001210668410029177 68-69 6.547492421586366E-4 70-71 0.0018036488557577536 72-73 0.0 74-75 0.0 76-77 0.10005309645741128 78-79 0.03672772635731371 80-81 3.7061277858036035E-5 82-83 0.0 84-85 0.0 86-87 0.15334721401726709 88-89 0.33027775451153113 90-91 0.28411175605970423 92-93 0.0 94-95 1.2353759286012011E-5 96-97 1.2353759286012011E-5 98-99 2.347214264342282E-4 100-101 6.176879643006005E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4047351.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.74518982818114 #Duplication Level Percentage of deduplicated Percentage of total 1 67.22899451946671 30.081741217312878 2 16.050398627875655 14.363562668445484 3 6.546490018560533 8.787718157663523 4 3.376974900889593 6.04413531941232 5 1.9000695167773984 4.250948560747255 6 1.198711050715539 3.2181932108043156 7 0.7832589265399679 2.4532948546853772 8 0.5541677378811936 1.9837072502518185 9 0.38915694752518876 1.5671611340973088 >10 1.761029463744848 13.74580666359092 >50 0.1301889177355398 4.018434494811799 >100 0.0748627387715484 6.082224319802874 >500 0.0040927270599012185 1.1764607852759084 >1k 0.0013826779797218222 1.133474711056766 >5k 1.1061423837774548E-4 0.39494176921870006 >10k+ 1.1061423837774548E-4 0.6981948828226823 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15380 0.3800016356377295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12833 0.3170715858347843 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8682 0.2145106762423126 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7277 0.17979661264861882 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4707518572024022E-5 2 0.0 0.0 0.0 0.0 2.4707518572024022E-5 3 0.0 0.0 0.0 0.0 2.4707518572024022E-5 4 0.0 0.0 0.0 0.0 2.4707518572024022E-5 5 2.4707518572024022E-5 0.0 0.0 0.0 2.4707518572024022E-5 6 2.4707518572024022E-5 0.0 0.0 4.9415037144048044E-5 4.9415037144048044E-5 7 2.4707518572024022E-5 0.0 0.0 4.9415037144048044E-5 4.9415037144048044E-5 8 2.4707518572024022E-5 0.0 0.0 4.9415037144048044E-5 4.9415037144048044E-5 9 2.4707518572024022E-5 0.0 0.0 2.470751857202402E-4 7.412255571607207E-5 10-11 4.9415037144048044E-5 0.0 0.0 3.5825901929434833E-4 9.883007428809609E-5 12-13 4.9415037144048044E-5 0.0 0.0 4.5708909358244446E-4 1.4824511143214414E-4 14-15 4.9415037144048044E-5 0.0 0.0 4.8179661215446844E-4 1.4824511143214414E-4 16-17 4.9415037144048044E-5 0.0 0.0 6.176879643006005E-4 1.8530638929018016E-4 18-19 4.9415037144048044E-5 0.0 0.0 7.659330757327447E-4 2.223676671482162E-4 20-21 4.9415037144048044E-5 0.0 0.0 9.018244278788768E-4 2.3472142643422822E-4 22-23 6.176879643006005E-5 0.0 0.0 0.001223022169315189 2.7178270429226425E-4 24-25 7.412255571607207E-5 0.0 0.0 0.0017418800593276937 2.964902228642883E-4 26-27 7.412255571607207E-5 0.0 0.0 0.0026684120057785944 2.964902228642883E-4 28-29 7.412255571607207E-5 0.0 0.0 0.005583899197277429 2.964902228642883E-4 30-31 7.412255571607207E-5 2.4707518572024022E-5 0.0 0.01455272843892215 2.964902228642883E-4 32-33 9.883007428809609E-5 2.4707518572024022E-5 0.0 0.030711445585025858 2.964902228642883E-4 34-35 9.883007428809609E-5 2.4707518572024022E-5 0.0 0.05591311452849036 3.211977414363123E-4 36-37 9.883007428809609E-5 2.4707518572024022E-5 0.0 0.10054724682885177 3.211977414363123E-4 38-39 9.883007428809609E-5 2.4707518572024022E-5 0.0 0.164576781208252 3.5825901929434833E-4 40-41 9.883007428809609E-5 2.4707518572024022E-5 0.0 0.23640153769712585 3.706127785803603E-4 42-43 9.883007428809609E-5 2.4707518572024022E-5 0.0 0.30925165620673867 3.706127785803603E-4 44-45 9.883007428809609E-5 2.4707518572024022E-5 0.0 0.401052441461094 3.706127785803603E-4 46-47 1.1118383357410809E-4 2.4707518572024022E-5 0.0 0.5146452580959744 4.200278157244084E-4 48-49 1.235375928601201E-4 2.4707518572024022E-5 0.0 0.6364039096189087 4.447353342964324E-4 50-51 1.235375928601201E-4 2.4707518572024022E-5 0.0 0.7677737858663605 4.941503714404804E-4 52-53 1.235375928601201E-4 2.4707518572024022E-5 0.0 0.8957463783101589 5.435654085845285E-4 54-55 1.235375928601201E-4 2.4707518572024022E-5 0.0 1.0416195679593887 5.806266864425646E-4 56-57 1.235375928601201E-4 2.4707518572024022E-5 0.0 1.2158693426885883 6.176879643006005E-4 58-59 1.235375928601201E-4 2.4707518572024022E-5 0.0 1.4089338928103838 6.176879643006005E-4 60-61 1.4824511143214414E-4 2.4707518572024022E-5 0.0 1.6224562683098154 6.300417235866125E-4 62-63 1.4824511143214414E-4 2.4707518572024022E-5 0.0 1.8362874877913975 6.423954828726246E-4 64-65 1.4824511143214414E-4 2.4707518572024022E-5 0.0 2.0566414921759937 6.423954828726246E-4 66-67 1.4824511143214414E-4 2.4707518572024022E-5 0.0 2.303827861729808 6.423954828726246E-4 68-69 1.4824511143214414E-4 2.4707518572024022E-5 0.0 2.5924610936881924 6.423954828726246E-4 70-71 1.7295263000416817E-4 2.4707518572024022E-5 0.0 2.9059624430893196 6.547492421586366E-4 72-73 1.7295263000416817E-4 2.4707518572024022E-5 0.0 3.2257642097263126 7.288717978747087E-4 74-75 1.7295263000416817E-4 2.4707518572024022E-5 0.0 3.5436882049518315 7.659330757327446E-4 76-77 1.7295263000416817E-4 2.4707518572024022E-5 0.0 3.896968659253917 7.906405943047687E-4 78-79 1.7295263000416817E-4 2.4707518572024022E-5 0.0 4.299021755217177 7.906405943047687E-4 80-81 1.7295263000416817E-4 2.4707518572024022E-5 0.0 4.737382549722028 8.400556314488168E-4 82-83 1.7295263000416817E-4 2.4707518572024022E-5 0.0 5.17704048895191 9.141781871648888E-4 84-85 1.7295263000416817E-4 2.4707518572024022E-5 0.0 5.61949037778043 9.141781871648888E-4 86-87 1.7295263000416817E-4 2.4707518572024022E-5 0.0 6.079593788628661 9.512394650229249E-4 88-89 1.7295263000416817E-4 2.4707518572024022E-5 0.0 6.580180468657154 9.883007428809609E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18180 0.0 23.320278 1 GTATCAA 27400 0.0 22.740227 1 TACATGG 18480 0.0 22.441597 2 GGTATCA 20980 0.0 22.410381 1 ACATGGG 18325 0.0 21.854595 3 GAGTACT 14855 0.0 20.970642 12-13 GTACTTT 15555 0.0 19.828644 14-15 TCAACGC 31755 0.0 19.425648 4 ATCAACG 31920 0.0 19.354965 3 CAACGCA 31950 0.0 19.277384 5 TATCAAC 32390 0.0 19.103413 2 AGTACTT 15395 0.0 19.07922 12-13 GTACCGT 275 2.8765498E-8 18.980352 6 AACGCAG 32580 0.0 18.962875 6 CATGGGA 11200 0.0 18.133017 4 AGAGTAC 27065 0.0 17.532303 10-11 CATGGGG 7270 0.0 17.2964 4 ACGCAGA 35710 0.0 17.221043 7 TACCTGG 3105 0.0 17.115938 2 CGCAGAG 35795 0.0 17.100613 8 >>END_MODULE