FastQCFastQC Report
Thu 26 May 2016
SRR938728_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938728_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4060079
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155780.38368711544775363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117670.2898219468143354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80240.19763162243887372No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64630.15918409469372394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG181000.025.7312871
GTATCAA254900.025.3374941
TACATGG186700.024.6203172
ACATGGG184450.023.8147753
TACCTGG31300.023.1878912
TCAACGC294850.021.8158784
GGTATCA186150.021.7260381
ATCAACG297750.021.60343
CAACGCA298900.021.5678945
GAGTACT137800.021.543712-13
AACGCAG303250.021.2890056
TATCAAC302400.021.2712022
GTACTTT143200.020.41693714-15
CATGGGG70550.020.104274
GTATAGG14550.019.9241981
AGTACTT145850.019.50854712-13
ACGCAGA334300.019.2125827
CGCAGAG335650.019.0646468
GTACCTG40800.018.986351
CATGGGA115550.018.9664574
Produced by FastQC (version 0.11.5)