##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938728_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4060079 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37997610391325 33.0 31.0 34.0 28.0 34.0 2 31.441757906681126 33.0 31.0 34.0 28.0 34.0 3 31.454600267630262 33.0 31.0 34.0 28.0 34.0 4 35.00073151285972 37.0 35.0 37.0 32.0 37.0 5 35.033705009188246 37.0 35.0 37.0 32.0 37.0 6 35.084423973031065 37.0 35.0 37.0 32.0 37.0 7 34.986067019878185 37.0 35.0 37.0 32.0 37.0 8 34.95003249936762 37.0 35.0 37.0 32.0 37.0 9 36.49405762794271 39.0 37.0 39.0 32.0 39.0 10-11 36.370404615279654 39.0 37.0 39.0 32.0 39.0 12-13 36.249901664475004 39.0 37.0 39.0 32.0 39.0 14-15 37.37055609016475 40.0 37.0 41.0 32.0 41.0 16-17 37.253883606698295 40.0 37.0 41.0 31.0 41.0 18-19 37.19589520302438 40.0 37.0 41.0 31.0 41.0 20-21 37.08240196804053 40.0 37.0 41.0 31.0 41.0 22-23 37.108444318447994 40.0 37.0 41.0 31.0 41.0 24-25 37.04608531016268 40.0 37.0 41.0 30.5 41.0 26-27 36.9096069312937 40.0 36.5 41.0 30.0 41.0 28-29 36.73943056280432 40.0 36.0 41.0 30.0 41.0 30-31 36.51223190484717 39.0 36.0 41.0 30.0 41.0 32-33 36.33030416403227 39.0 35.5 41.0 29.5 41.0 34-35 36.04478533545776 39.0 35.0 41.0 28.5 41.0 36-37 35.83971358685385 39.0 35.0 41.0 27.0 41.0 38-39 35.673726422564684 39.0 35.0 40.5 27.0 41.0 40-41 35.56857859169735 39.0 35.0 40.5 26.5 41.0 42-43 35.556426611403374 39.0 35.0 41.0 26.0 41.0 44-45 35.38510371349917 39.0 35.0 41.0 25.5 41.0 46-47 35.08925577556496 39.0 35.0 41.0 24.0 41.0 48-49 34.820523689317376 39.0 34.0 41.0 24.0 41.0 50-51 33.70469343084211 38.0 32.5 39.5 22.0 40.5 52-53 33.785283488326215 38.0 33.0 39.5 22.0 40.5 54-55 34.286880501586296 38.0 34.0 40.0 22.5 41.0 56-57 34.187531449511205 38.0 34.0 40.0 22.0 41.0 58-59 33.98071022755961 38.0 33.5 40.0 21.5 41.0 60-61 33.66925655880095 38.0 33.0 40.0 20.0 41.0 62-63 33.276402996099335 37.0 33.0 40.0 18.5 41.0 64-65 32.890412354045324 36.5 32.0 40.0 16.0 41.0 66-67 32.503776034899815 36.0 32.0 39.0 13.0 41.0 68-69 32.07850463008232 35.5 31.0 39.0 9.5 40.5 70-71 31.65627639265147 35.0 31.0 38.5 8.0 40.0 72-73 31.226789798917707 35.0 31.0 37.5 7.0 40.0 74-75 30.757841165159597 35.0 30.0 37.0 2.0 39.0 76-77 30.294331957580134 35.0 30.0 36.5 2.0 39.0 78-79 29.85234609966949 34.0 29.5 36.0 2.0 38.5 80-81 29.37341908864335 34.0 29.0 35.5 2.0 37.5 82-83 28.945297369829504 34.0 29.0 35.0 2.0 37.0 84-85 28.577946759164046 34.0 29.0 35.0 2.0 36.5 86-87 28.148944023010387 34.0 27.5 35.0 2.0 36.0 88-89 27.684431386679915 34.0 26.5 35.0 2.0 36.0 90-91 27.22272534598465 34.0 26.0 35.0 2.0 35.0 92-93 26.750878246457766 33.0 25.0 35.0 2.0 35.0 94-95 26.223066964953144 33.0 24.5 35.0 2.0 35.0 96-97 25.604450923245583 33.0 21.5 35.0 2.0 35.0 98-99 24.814780574466653 32.0 10.0 35.0 2.0 35.0 100-101 22.270786726071094 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1824037942681258 1101 2 0.3252351622248071 1101 3 0.3610855526133463 1101 4 0.20449133505023553 1101 5 0.19437326639123143 1101 6 0.3402041747243132 1101 7 0.41877775004545725 1101 8 0.40793451143857595 1101 9 0.4675674043886531 1101 10-11 0.41560637019079394 1101 12-13 0.4729618462136287 1101 14-15 0.505570547247487 1101 16-17 0.6766578069504448 1101 18-19 0.6254097021497245 1101 20-21 0.8027666973237118 1101 22-23 0.6760254638469974 1101 24-25 0.7239443398696395 1101 26-27 0.7183122088059264 1101 28-29 0.7648884385372483 1101 30-31 0.7034142014390241 1101 32-33 0.6810334807268319 1101 34-35 0.7819913968977872 1101 36-37 0.7412956043382763 1101 38-39 0.7183343023120159 1101 40-41 0.9151245462065134 1101 42-43 0.9662413629004689 1101 44-45 1.1468333634640686 1101 46-47 1.037212466442334 1101 48-49 1.0231411584887908 1101 50-51 1.030754952109156 1101 52-53 1.086097278907296 1101 54-55 1.0766508767682197 1101 56-57 0.9637393206366909 1101 58-59 0.9977983161677741 1101 60-61 0.9651556729304573 1101 62-63 0.9503615561566505 1101 64-65 1.0016711833746896 1101 66-67 0.9860513359142118 1101 68-69 0.9864030508489705 1101 70-71 0.8714832020937884 1101 72-73 0.9046743471932537 1101 74-75 0.7590319165570705 1101 76-77 0.7631250850081379 1101 78-79 0.672919386122178 1101 80-81 0.6969359457303561 1101 82-83 0.8407449944449716 1101 84-85 0.6827466274279423 1101 86-87 0.5073605327877182 1101 88-89 0.6496823748294247 1101 90-91 0.5525635409266307 1101 92-93 0.4314530894921127 1101 94-95 0.4483258983550762 1101 96-97 0.5130177521057782 1101 98-99 0.4627906194763973 1101 100-101 0.8665080929732021 1102 1 0.18972558088802316 1102 2 0.1544301140437092 1102 3 0.14919323554385144 1102 4 0.29842231648117234 1102 5 0.2707574813224696 1102 6 0.3647057902065143 1102 7 0.3662439400722235 1102 8 0.39204970350507296 1102 9 0.5503918892545983 1102 10-11 0.4852266050778056 1102 12-13 0.45982103872341185 1102 14-15 0.45810419477906095 1102 16-17 0.6839755325291037 1102 18-19 0.6354467009427864 1102 20-21 0.6523934695846734 1102 22-23 0.5856746061280163 1102 24-25 0.5624851915078892 1102 26-27 0.5663720508657235 1102 28-29 0.6505335782560167 1102 30-31 0.723798913802689 1102 32-33 0.6590338276299974 1102 34-35 0.8107710937130932 1102 36-37 0.902763326009314 1102 38-39 0.797750490633149 1102 40-41 0.8645564606842342 1102 42-43 0.892300089888586 1102 44-45 0.9091839374516297 1102 46-47 0.7955626440722767 1102 48-49 0.7113601319983474 1102 50-51 0.7753126604978746 1102 52-53 0.632773082014296 1102 54-55 0.6037558904214393 1102 56-57 0.6818667799355751 1102 58-59 0.5882358094332147 1102 60-61 0.5998031953072456 1102 62-63 0.391904130939821 1102 64-65 0.42176568470679854 1102 66-67 0.3909431037504447 1102 68-69 0.4476636955148976 1102 70-71 0.44220000836100226 1102 72-73 0.31371884555629137 1102 74-75 0.23879114554402392 1102 76-77 0.2841705739042588 1102 78-79 0.2533509249987027 1102 80-81 0.25053654611469156 1102 82-83 0.3078034523214157 1102 84-85 0.3038686263410284 1102 86-87 0.23752726470177876 1102 88-89 0.3461965214214615 1102 90-91 0.2918229441773761 1102 92-93 0.13849354522396595 1102 94-95 0.14529903285260914 1102 96-97 0.11239007757169972 1102 98-99 -0.06390675588167483 1102 100-101 0.08931450349826875 1103 1 0.11673215463693154 1103 2 0.23226214270991719 1103 3 0.33799758982122086 1103 4 0.24267166271744856 1103 5 0.2651099423844627 1103 6 0.19712399733860053 1103 7 0.19673267717303844 1103 8 0.3183104467184066 1103 9 0.46965412484408375 1103 10-11 0.31414564778322074 1103 12-13 0.420503148301151 1103 14-15 0.44973639449289493 1103 16-17 0.4951394464875776 1103 18-19 0.5178614756812223 1103 20-21 0.4998838177652516 1103 22-23 0.5077187788889646 1103 24-25 0.5185684299140831 1103 26-27 0.5510134300872949 1103 28-29 0.5328487778246824 1103 30-31 0.6303031031106414 1103 32-33 0.5328652942417449 1103 34-35 0.5682333320198936 1103 36-37 0.693952139123347 1103 38-39 0.6968505420475495 1103 40-41 0.7287557726323257 1103 42-43 0.7648418167115238 1103 44-45 0.7868809586345122 1103 46-47 0.8172699416987044 1103 48-49 0.8500674496470708 1103 50-51 0.8271580726389587 1103 52-53 0.7306694180055899 1103 54-55 0.7190483839910584 1103 56-57 0.7659682007320185 1103 58-59 0.8817180693211881 1103 60-61 0.738898087404948 1103 62-63 0.6137437544920488 1103 64-65 0.5489814556975006 1103 66-67 0.5761202939214982 1103 68-69 0.6017376786146471 1103 70-71 0.5316910022606329 1103 72-73 0.5779146536025372 1103 74-75 0.40672717216223475 1103 76-77 0.4355575469692603 1103 78-79 0.39113551869983 1103 80-81 0.44783875621866187 1103 82-83 0.5336944623819022 1103 84-85 0.6461184066320804 1103 86-87 0.5271610426015947 1103 88-89 0.5953348476004017 1103 90-91 0.6235043486842535 1103 92-93 0.4837112733941211 1103 94-95 0.504612993899233 1103 96-97 0.6505931551416531 1103 98-99 0.6492395262996986 1103 100-101 1.015311828376472 1104 1 0.039364230635339226 1104 2 0.10314613802010797 1104 3 0.06346101147175887 1104 4 0.22100707315634338 1104 5 0.17541494096976606 1104 6 0.23603755284179329 1104 7 0.21024853083503814 1104 8 0.20128849563968032 1104 9 0.2756728102775696 1104 10-11 0.22401227773385557 1104 12-13 0.34225765902687044 1104 14-15 0.42985508759131363 1104 16-17 0.48582653786792207 1104 18-19 0.4678501911783215 1104 20-21 0.5196800768934011 1104 22-23 0.4323819471231616 1104 24-25 0.3471046666530242 1104 26-27 0.34601257369524774 1104 28-29 0.3390500971415733 1104 30-31 0.43270045671728496 1104 32-33 0.46754008764307997 1104 34-35 0.595093997507476 1104 36-37 0.5951000120063767 1104 38-39 0.6086400323665373 1104 40-41 0.5165836974477287 1104 42-43 0.6417444501407132 1104 44-45 0.664881406229469 1104 46-47 0.613888068442833 1104 48-49 0.6819474005157176 1104 50-51 0.7990247659909997 1104 52-53 0.8618161600725998 1104 54-55 0.7669917892518683 1104 56-57 0.7596624820203743 1104 58-59 0.8035791559076273 1104 60-61 0.7614154021380131 1104 62-63 0.8362195034565687 1104 64-65 0.8790348311596432 1104 66-67 0.7925888785278232 1104 68-69 0.8252964559706939 1104 70-71 0.7893385595561035 1104 72-73 0.5977145796026981 1104 74-75 0.5900329211551494 1104 76-77 0.5870844088176135 1104 78-79 0.4485280982756841 1104 80-81 0.4772580703669185 1104 82-83 0.4891730767111895 1104 84-85 0.5591910655827022 1104 86-87 0.48252231702651116 1104 88-89 0.6271421234315007 1104 90-91 0.607087289543994 1104 92-93 0.6557959208870656 1104 94-95 0.6934813877335877 1104 96-97 0.6593908091005964 1104 98-99 0.8161612180004525 1104 100-101 1.1559996531312144 1105 1 0.08481166832043385 1105 2 0.15017260084547246 1105 3 0.30035292875830777 1105 4 0.28619028871624863 1105 5 0.18545417251965546 1105 6 0.27847877951493416 1105 7 0.24606091509129868 1105 8 0.21837617831431544 1105 9 0.3078835005422107 1105 10-11 0.2819653455754363 1105 12-13 0.37729435514205534 1105 14-15 0.4451322576423493 1105 16-17 0.44110958645624976 1105 18-19 0.4270305815067772 1105 20-21 0.48473131791411816 1105 22-23 0.45749623098217285 1105 24-25 0.4595111280420241 1105 26-27 0.46018580859100666 1105 28-29 0.5328335439423029 1105 30-31 0.5378152201290973 1105 32-33 0.4940983073044194 1105 34-35 0.4403387495591673 1105 36-37 0.5435874932688236 1105 38-39 0.5510002226457473 1105 40-41 0.6004121671442348 1105 42-43 0.53837188028605 1105 44-45 0.4822325045086515 1105 46-47 0.7164760015633433 1105 48-49 0.7340574386114866 1105 50-51 0.88553181920323 1105 52-53 0.8678647780082969 1105 54-55 0.7996508556566155 1105 56-57 0.7088248363447391 1105 58-59 0.6467365052150384 1105 60-61 0.7941408946376072 1105 62-63 0.7356640195407493 1105 64-65 0.7363637116883552 1105 66-67 0.6815091321080047 1105 68-69 0.7446127570720407 1105 70-71 0.6465858988984543 1105 72-73 0.669910910817201 1105 74-75 0.6419562434144055 1105 76-77 0.6461545019999981 1105 78-79 0.6906025703029606 1105 80-81 0.7918165766126926 1105 82-83 0.7516861640285768 1105 84-85 0.684360056321367 1105 86-87 0.7149466778961973 1105 88-89 0.8299094701071112 1105 90-91 0.8108675768030089 1105 92-93 0.7522395262798085 1105 94-95 0.919076337239435 1105 96-97 0.8856885099775802 1105 98-99 0.8355306771864974 1105 100-101 1.2790023099455574 1106 1 0.10591565986524643 1106 2 0.10000254683539822 1106 3 0.10871559272569797 1106 4 0.1292567079818454 1106 5 0.2308885487972816 1106 6 0.12545706268421242 1106 7 0.1339125275593247 1106 8 0.14696165176984977 1106 9 0.11276339492211207 1106 10-11 0.21255437499468854 1106 12-13 0.15000081170347102 1106 14-15 0.3268305270932146 1106 16-17 0.38067710641881547 1106 18-19 0.26431446680700077 1106 20-21 0.3292873026264971 1106 22-23 0.2704721229928566 1106 24-25 0.153729949940427 1106 26-27 0.09321798638896439 1106 28-29 0.19825398801638272 1106 30-31 -0.10756949552797579 1106 32-33 0.12420890809440976 1106 34-35 0.12183278991901858 1106 36-37 0.35418359118289544 1106 38-39 0.40445328981495265 1106 40-41 0.5107573346178782 1106 42-43 0.5647988748699575 1106 44-45 0.5619767323405682 1106 46-47 0.5330144115807158 1106 48-49 0.7187547437112727 1106 50-51 0.5608848374657001 1106 52-53 0.5118568077796866 1106 54-55 0.3681752544815353 1106 56-57 0.5679728126730481 1106 58-59 0.6875555437028424 1106 60-61 0.705474914379316 1106 62-63 0.6906227312383635 1106 64-65 0.6048498762799781 1106 66-67 0.602643740294603 1106 68-69 0.6174101746522567 1106 70-71 0.5857087124204305 1106 72-73 0.5978791005652155 1106 74-75 0.4027083134836431 1106 76-77 0.5485571207411759 1106 78-79 0.547355449839749 1106 80-81 0.5448796613535585 1106 82-83 0.590572303370756 1106 84-85 0.62006074843638 1106 86-87 0.5927816638736978 1106 88-89 0.723623758779059 1106 90-91 0.8080843991680347 1106 92-93 0.9070230783807318 1106 94-95 0.9793305858892083 1106 96-97 0.9509289054689809 1106 98-99 0.8280205139748169 1106 100-101 1.335555556825792 1107 1 -0.01610420105739152 1107 2 0.07221230180463678 1107 3 0.17858161127211147 1107 4 0.21306863994087877 1107 5 0.17824170956639307 1107 6 0.07139357226872534 1107 7 0.00945318993316846 1107 8 0.05993817662077561 1107 9 0.116008798710304 1107 10-11 0.12828585753613453 1107 12-13 0.14010003446819752 1107 14-15 0.19897330086596554 1107 16-17 0.28038806921352943 1107 18-19 0.26549479456189573 1107 20-21 0.16953625202295797 1107 22-23 0.2777799856816259 1107 24-25 0.20003787777160653 1107 26-27 0.15393606540322935 1107 28-29 0.22997789273535574 1107 30-31 0.08570480861710905 1107 32-33 0.2976198229348981 1107 34-35 0.3000846210541894 1107 36-37 0.2729560805905251 1107 38-39 0.243963276505049 1107 40-41 0.2858844211791407 1107 42-43 0.2798474777222637 1107 44-45 0.39445355324750153 1107 46-47 0.5090620186847588 1107 48-49 0.4250531008780172 1107 50-51 0.41649410903566064 1107 52-53 0.39647931424655525 1107 54-55 0.35131990841444605 1107 56-57 0.4169079585275739 1107 58-59 0.542242758669758 1107 60-61 0.5258660805304629 1107 62-63 0.46863458379317535 1107 64-65 0.4155487545411489 1107 66-67 0.1858548428483715 1107 68-69 0.3598137961217205 1107 70-71 0.48738395430227044 1107 72-73 0.38346193620195024 1107 74-75 0.38242124624759555 1107 76-77 0.38716640991416185 1107 78-79 0.46392573900365264 1107 80-81 0.398007663047931 1107 82-83 0.3390578544817018 1107 84-85 0.46694920613209945 1107 86-87 0.3790239122353576 1107 88-89 0.5432902576750571 1107 90-91 0.6691136368975741 1107 92-93 0.8083902673041479 1107 94-95 0.8413418370259755 1107 96-97 0.9660747958743308 1107 98-99 1.2260244877738913 1107 100-101 1.4970828312573055 1108 1 0.017532157086467492 1108 2 0.07864307125165482 1108 3 0.0042239145023437175 1108 4 0.042105361107871886 1108 5 0.13833986711487967 1108 6 0.1393492534105718 1108 7 0.023721717805450737 1108 8 0.1445343907588068 1108 9 0.18094058175410765 1108 10-11 0.21796001215597727 1108 12-13 0.2360767870301217 1108 14-15 0.28615661891611666 1108 16-17 0.31683433637719816 1108 18-19 0.33506501127715893 1108 20-21 0.3670672212547643 1108 22-23 0.3423028097653429 1108 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2316 60-61 -1.6751671275882387 2316 62-63 -1.9558486247004225 2316 64-65 -2.1142646981584647 2316 66-67 -1.8137159034158046 2316 68-69 -2.148998089520589 2316 70-71 -2.0049063486871326 2316 72-73 -1.988326158661252 2316 74-75 -2.0885075913557785 2316 76-77 -2.0429608291408066 2316 78-79 -1.8309584562111993 2316 80-81 -1.7740968424292696 2316 82-83 -1.7590181890360057 2316 84-85 -1.778300885716952 2316 86-87 -1.9201603182195406 2316 88-89 -1.869878647109342 2316 90-91 -2.1938906893802503 2316 92-93 -2.0057578608496485 2316 94-95 -1.9597899682390363 2316 96-97 -2.293914257218166 2316 98-99 -2.5369299775896472 2316 100-101 -2.635303519598576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22796.0 3 6159.0 4 5823.0 5 6582.0 6 7402.0 7 8851.0 8 10674.0 9 12484.0 10 15134.0 11 17557.0 12 21145.0 13 24779.0 14 26347.0 15 28500.0 16 32825.0 17 38600.0 18 35946.0 19 28929.0 20 29653.0 21 32096.0 22 35009.0 23 39088.0 24 44363.0 25 50078.0 26 57277.0 27 66001.0 28 76797.0 29 89807.0 30 105925.0 31 126596.0 32 154288.0 33 192502.0 34 252216.0 35 348565.0 36 502022.0 37 733653.0 38 667220.0 39 106366.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21716051565916 18.901171584410093 14.043650632916393 22.83801726701435 2 15.170476006500339 20.341882113138173 40.503319865384654 23.984322014976833 3 19.362835099273685 25.081189823287513 29.585053373054652 25.970921704384153 4 12.482104436816789 16.516022804677284 37.82676132678439 33.17511143172153 5 13.41005079192494 37.83142946938943 34.30243624962874 14.456083489056887 6 31.42153020590041 36.36761189874967 17.818386517856613 14.392471377493301 7 28.310961918969113 32.88267068418851 20.966269309263755 17.840098087578617 8 25.973565497369144 35.061691205700726 20.012246883341028 18.952496413589103 9 26.528446055381984 16.92513037888442 20.62638105908454 35.92004250664906 10-11 24.776671813045162 26.813253099026053 27.48937394805387 20.920701139874915 12-13 25.916051481658236 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29.106409917966452 21.94978822374404 38-39 21.96527240018949 27.066193043304544 29.196336119294937 21.77219843721103 40-41 22.054018638093464 26.93202281097802 29.25492607489344 21.759032476035078 42-43 21.82972249545058 26.9988376274447 28.872107990999186 22.299331886105538 44-45 22.010904033379695 27.067853500231802 28.751995054860146 22.169247411528357 46-47 22.194236630268772 27.16878687198244 28.46573607842153 22.17124041932725 48-49 22.26250785394634 27.246036979984044 28.318056601340054 22.173398564729563 50-51 22.0405976419621 27.44533199329271 28.352888850529784 22.161181514215407 52-53 22.23446906672945 27.486465272158572 28.138224366367993 22.140841294743986 54-55 21.987788539100563 27.263517005588163 28.322653889581012 22.42604056573026 56-57 22.148301440703243 27.5574995077007 28.058439542864406 22.235759508731654 58-59 22.2996751221258 27.20546943666414 28.21240794220615 22.28244749900391 60-61 22.195632203333716 27.259739804084642 28.489251241210717 22.05537675137093 62-63 22.077268139023555 27.281076593753497 28.51316844370241 22.12848682352054 64-65 21.944061172758182 27.298631488200158 28.397445339109183 22.359861999932477 66-67 21.92888299530785 27.45564880325387 28.040006267907536 22.575461933530747 68-69 21.893715292005712 27.36846753170294 28.23690098851806 22.50091618777329 70-71 22.026798950482867 27.16431988059232 28.224300521864404 22.58458064706041 72-73 22.035815417812533 27.126442268628864 28.200105887254683 22.637636426303917 74-75 21.863599601302937 27.495319266812924 27.99422235523117 22.646858776652973 76-77 22.06505960933076 27.18982993844944 28.088878612602407 22.65623183961739 78-79 21.988045707849402 27.157620682008265 28.18410905368158 22.670224556460756 80-81 22.043524798038202 27.354477849044113 27.96945594373331 22.63254140918438 82-83 22.14728747536187 27.26326440633456 27.99363606218242 22.595812056121147 84-85 22.035973986682265 27.235521811233937 27.88265248402852 22.84585171805528 86-87 22.03378305367887 27.24935383042008 28.02540021962224 22.69146289627881 88-89 22.165378723724153 27.083425293930794 27.86029605686559 22.89089992547946 90-91 22.163519604489466 27.070324664226387 27.981954881266947 22.784200850017204 92-93 22.337871484419008 27.12185780949115 27.90388470795157 22.636385998138273 94-95 22.141520327230417 26.974333210641717 27.96454855421782 22.919597907910045 96-97 22.163595863599202 26.809452722165812 27.996039721518954 23.030911692716032 98-99 22.17162962259372 26.887538488633485 27.721463003419455 23.219368885353344 100-101 22.5333776802914 26.335094847262646 28.11846078138021 23.013066691065738 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5074.0 1 3328.0 2 1677.0 3 1768.0 4 1617.5 5 1703.5 6 2626.5 7 4578.0 8 6641.5 9 7146.5 10 6014.0 11 4861.5 12 4664.5 13 4855.0 14 4967.0 15 5174.5 16 5469.0 17 5781.0 18 6066.5 19 6514.0 20 7298.0 21 8411.0 22 9950.0 23 11848.0 24 14163.0 25 17522.0 26 22463.0 27 28433.0 28 33432.0 29 39349.5 30 47608.5 31 55633.0 32 64781.0 33 74154.0 34 82924.5 35 92334.5 36 103400.0 37 113103.0 38 117625.5 39 119398.0 40 121443.5 41 125417.0 42 128722.0 43 133881.0 44 140945.5 45 144616.5 46 145683.0 47 151974.5 48 169615.5 49 169430.5 50 153341.5 51 144309.0 52 135992.0 53 131851.5 54 127817.0 55 118580.0 56 107325.0 57 96165.0 58 86813.5 59 76593.0 60 64874.0 61 53986.5 62 43444.5 63 33715.5 64 25344.5 65 19067.0 66 14329.0 67 10987.5 68 8164.5 69 5685.0 70 4185.0 71 3187.5 72 2517.0 73 2088.5 74 1642.0 75 1275.0 76 959.5 77 636.0 78 447.5 79 307.5 80 216.5 81 171.5 82 128.5 83 98.0 84 72.5 85 49.0 86 28.5 87 23.5 88 23.0 89 14.0 90 16.5 91 16.0 92 9.0 93 8.0 94 4.5 95 4.0 96 6.5 97 4.0 98 2.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27593059149834276 2 0.1663021827900393 3 0.19327210135566328 4 0.16502141953395488 5 0.2372367631270229 6 0.19760699237625673 7 0.24189184496163746 8 0.24800010049065546 9 0.15085913352917518 10-11 0.205968898634731 12-13 0.2131855069815144 14-15 0.17510743017561972 16-17 0.28733431049001756 18-19 0.23409643014335435 20-21 0.200008423481415 22-23 0.24643609151447549 24-25 0.23612841030925752 26-27 0.3454243131722314 28-29 0.317222891475757 30-31 0.2922356929508022 32-33 0.19482379530053479 34-35 0.3631825883190943 36-37 0.4016301160642441 38-39 0.3821354215028821 40-41 0.3437617839455833 42-43 0.3776896952005121 44-45 0.38679050333749665 46-47 0.392467732770717 48-49 0.4120486325512386 50-51 0.404437943202583 52-53 0.40410543735725335 54-55 0.39834200270487347 56-57 0.3764335620070447 58-59 0.35100302235498376 60-61 0.315782032812662 62-63 0.3856205753631887 64-65 0.41963469183727703 66-67 0.4251764559261039 68-69 0.43133397158035597 70-71 0.429991633167729 72-73 0.42116175571953157 74-75 0.4164697287909915 76-77 0.40021388746376607 78-79 0.3736873100252483 80-81 0.38486935845337 82-83 0.3951154645020454 84-85 0.39589131147448114 86-87 0.39068205323098393 88-89 0.39968434111750045 90-91 0.4212233308760741 92-93 0.42017655321485126 94-95 0.42912958097613374 96-97 0.42940051166492077 98-99 0.4267650949649009 100-101 0.3992286849590858 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4060079.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.472163922052616 #Duplication Level Percentage of deduplicated Percentage of total 1 70.08756424730574 33.272083388454924 2 14.807483864206079 14.058866025494801 3 6.019501970731929 8.572763528511148 4 3.0142790443994354 5.7237739561015255 5 1.7338902575396375 4.115576126438585 6 1.053481261374455 3.0006621077267335 7 0.7078774186241813 2.3523130997552615 8 0.4649573484633561 1.7658025170412306 9 0.341197464369182 1.4577643762490267 >10 1.5852046741606542 13.093468806597592 >50 0.11560778151381869 3.8007553053615104 >100 0.06374577484975497 5.52646129086136 >500 0.0038545804805089267 1.1953389414652187 >1k 0.001145956291916321 1.029959768495853 >5k 1.041778447196648E-4 0.35823074921250764 >10k+ 1.041778447196648E-4 0.6761800122327619 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15578 0.38368711544775363 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11767 0.2898219468143354 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8024 0.19763162243887372 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6463 0.15918409469372394 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.926012523401638E-5 2 0.0 0.0 0.0 0.0 4.926012523401638E-5 3 0.0 0.0 0.0 2.463006261700819E-5 4.926012523401638E-5 4 0.0 0.0 0.0 2.463006261700819E-5 4.926012523401638E-5 5 0.0 0.0 0.0 2.463006261700819E-5 4.926012523401638E-5 6 0.0 0.0 0.0 2.463006261700819E-5 4.926012523401638E-5 7 0.0 0.0 0.0 2.463006261700819E-5 4.926012523401638E-5 8 0.0 0.0 0.0 2.463006261700819E-5 4.926012523401638E-5 9 0.0 0.0 0.0 1.7241043831905733E-4 4.926012523401638E-5 10-11 2.463006261700819E-5 0.0 0.0 3.3250584532961054E-4 4.926012523401638E-5 12-13 2.463006261700819E-5 0.0 0.0 3.9408100187213104E-4 1.7241043831905733E-4 14-15 2.463006261700819E-5 0.0 0.0 3.9408100187213104E-4 1.7241043831905733E-4 16-17 2.463006261700819E-5 0.0 0.0 4.926012523401638E-4 1.8472546962756143E-4 18-19 2.463006261700819E-5 0.0 0.0 6.280665967337089E-4 2.216705635530737E-4 20-21 2.463006261700819E-5 0.0 0.0 7.881620037442621E-4 2.58615657478586E-4 22-23 3.6945093925512285E-5 0.0 0.0 0.0011206678490738727 3.078757827126024E-4 24-25 8.620521915952867E-5 0.0 0.0 0.0014778037570204916 3.3250584532961054E-4 26-27 9.852025046803276E-5 0.0 0.0 0.0021058703537542005 3.571359079466188E-4 28-29 9.852025046803276E-5 0.0 0.0 0.00442109623975297 3.9408100187213104E-4 30-31 9.852025046803276E-5 0.0 0.0 0.012438181621589137 4.310260957976433E-4 32-33 9.852025046803276E-5 0.0 0.0 0.02746251981796413 5.17231314957172E-4 34-35 1.2315031308504095E-4 0.0 0.0 0.05120590018076003 5.17231314957172E-4 36-37 1.4778037570204914E-4 0.0 0.0 0.09182087343620654 5.17231314957172E-4 38-39 1.4778037570204914E-4 0.0 0.0 0.15265712810021675 5.17231314957172E-4 40-41 1.7241043831905733E-4 0.0 0.0 0.22034054017175528 5.17231314957172E-4 42-43 1.7241043831905733E-4 0.0 0.0 0.2898958370021864 5.17231314957172E-4 44-45 1.8472546962756143E-4 0.0 0.0 0.37585475553554504 5.418613775741803E-4 46-47 1.9704050093606552E-4 0.0 0.0 0.48226894107232887 5.664914401911884E-4 48-49 1.9704050093606552E-4 0.0 0.0 0.5959366800498217 6.157515654252048E-4 50-51 1.9704050093606552E-4 0.0 0.0 0.7222889012750737 6.403816280422129E-4 52-53 2.58615657478586E-4 0.0 0.0 0.8453406941096466 7.635319411272538E-4 54-55 2.709306887870901E-4 0.0 0.0 0.9855350105256573 7.881620037442621E-4 56-57 2.709306887870901E-4 0.0 0.0 1.1552607720194605 8.004770350527662E-4 58-59 2.709306887870901E-4 0.0 0.0 1.3433359301629353 8.127920663612703E-4 60-61 2.709306887870901E-4 0.0 0.0 1.5521249709673137 8.127920663612703E-4 62-63 2.709306887870901E-4 0.0 0.0 1.7603844654254264 8.866822542122948E-4 64-65 2.709306887870901E-4 0.0 0.0 1.975675842760695 9.113123168293031E-4 66-67 2.709306887870901E-4 0.0 0.0 2.2210035814574054 9.113123168293031E-4 68-69 2.709306887870901E-4 0.0 0.0 2.4964785167973336 9.113123168293031E-4 70-71 2.709306887870901E-4 0.0 0.0 2.8075192625562213 9.359423794463112E-4 72-73 2.709306887870901E-4 0.0 0.0 3.120505783261853 0.00102214759860584 74-75 2.709306887870901E-4 0.0 0.0 3.4381350707707905 0.0010714077238398562 76-77 2.709306887870901E-4 0.0 0.0 3.7758994344691326 0.0011083528177653686 78-79 2.709306887870901E-4 1.2315031308504095E-5 0.0 4.164635712753372 0.0011329828803823769 80-81 2.709306887870901E-4 2.463006261700819E-5 0.0 4.593469732978102 0.0011329828803823769 82-83 2.8324572009559416E-4 2.463006261700819E-5 0.0 5.026700219379968 0.0011576129429993849 84-85 2.955607514040983E-4 2.463006261700819E-5 0.0 5.463132613922045 0.0011576129429993849 86-87 2.955607514040983E-4 2.463006261700819E-5 0.0 5.922360131416163 0.0011945580369248972 88-89 2.955607514040983E-4 2.463006261700819E-5 0.0 6.417719950769431 0.0012315031308504096 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18100 0.0 25.731287 1 GTATCAA 25490 0.0 25.337494 1 TACATGG 18670 0.0 24.620317 2 ACATGGG 18445 0.0 23.814775 3 TACCTGG 3130 0.0 23.187891 2 TCAACGC 29485 0.0 21.815878 4 GGTATCA 18615 0.0 21.726038 1 ATCAACG 29775 0.0 21.6034 3 CAACGCA 29890 0.0 21.567894 5 GAGTACT 13780 0.0 21.5437 12-13 AACGCAG 30325 0.0 21.289005 6 TATCAAC 30240 0.0 21.271202 2 GTACTTT 14320 0.0 20.416937 14-15 CATGGGG 7055 0.0 20.10427 4 GTATAGG 1455 0.0 19.924198 1 AGTACTT 14585 0.0 19.508547 12-13 ACGCAGA 33430 0.0 19.212582 7 CGCAGAG 33565 0.0 19.064646 8 GTACCTG 4080 0.0 18.98635 1 CATGGGA 11555 0.0 18.966457 4 >>END_MODULE