FastQCFastQC Report
Thu 26 May 2016
SRR938728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4060079
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153530.37814535135892674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126020.3103880490995372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88000.21674455102967208No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73490.1810063301723932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG175550.024.0869671
GTATCAA268300.023.9505711
TACATGG179750.023.1807062
ACATGGG180250.022.2212353
GGTATCA199600.021.708961
GAGTACT147100.020.62556512-13
TCAACGC309950.020.5017034
ATCAACG311200.020.4041043
CAACGCA312850.020.2964925
TATCAAC316350.020.2069512
AACGCAG318500.020.0209126
GTACTTT150750.019.9058714-15
AGTACTT153850.019.65896612-13
CATGGGG70050.019.4435164
TACCTGG29150.018.7223472
GTGTAGC27100.018.4289761
CATGGGA110900.018.1440964
AGAGTAC267400.018.14177910-11
ACGCAGA351500.018.0859537
CGCAGAG353200.017.918318
Produced by FastQC (version 0.11.5)