##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938728_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4060079 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10098473453349 33.0 31.0 34.0 30.0 34.0 2 32.30811494062061 34.0 31.0 34.0 30.0 34.0 3 32.39139460094249 34.0 31.0 34.0 30.0 34.0 4 35.99273191482235 37.0 35.0 37.0 35.0 37.0 5 35.90286174234541 37.0 35.0 37.0 35.0 37.0 6 35.9269580715055 37.0 35.0 37.0 35.0 37.0 7 35.85030291282509 37.0 35.0 37.0 35.0 37.0 8 35.83668150299538 37.0 35.0 37.0 33.0 37.0 9 37.495422379712316 39.0 37.0 39.0 34.0 39.0 10-11 37.42446046000583 39.0 37.0 39.0 34.0 39.0 12-13 37.34804248882842 39.0 37.0 39.0 34.0 39.0 14-15 38.585390087237215 40.0 38.0 41.0 34.0 41.0 16-17 38.513193955092994 40.0 38.0 41.0 34.0 41.0 18-19 38.661246739287584 40.0 38.0 41.0 34.0 41.0 20-21 38.664707632536214 40.0 38.0 41.0 34.0 41.0 22-23 38.58899863771124 40.0 38.0 41.0 34.0 41.0 24-25 38.522955710960304 40.0 38.0 41.0 34.0 41.0 26-27 38.35667902028507 40.0 38.0 41.0 34.0 41.0 28-29 38.198907582832746 40.0 38.0 41.0 33.0 41.0 30-31 37.98943148643167 40.0 37.0 41.0 33.0 41.0 32-33 37.844949815015916 40.0 37.0 41.0 33.0 41.0 34-35 37.819325313620745 40.0 37.0 41.0 33.0 41.0 36-37 37.718527521262516 40.0 37.0 41.0 33.0 41.0 38-39 37.60299072998333 40.0 37.0 41.0 32.5 41.0 40-41 37.4455175625893 40.0 37.0 41.0 32.0 41.0 42-43 37.25815310490263 40.0 36.0 41.0 31.0 41.0 44-45 37.031461703085085 40.0 36.0 41.0 31.0 41.0 46-47 36.74712634901932 40.0 36.0 41.0 30.5 41.0 48-49 36.46218337130878 39.0 35.5 41.0 30.0 41.0 50-51 36.24550495199724 39.0 35.0 41.0 30.0 41.0 52-53 36.03284936081293 39.0 35.0 41.0 29.0 41.0 54-55 35.77183288798075 39.0 35.0 40.0 28.0 41.0 56-57 35.5152364030355 38.0 35.0 40.0 28.0 41.0 58-59 35.221276236250574 38.0 34.0 40.0 27.5 41.0 60-61 34.8666103541335 38.0 34.0 40.0 26.5 41.0 62-63 34.51549908265332 37.0 34.0 40.0 26.0 41.0 64-65 34.123335161704986 37.0 33.5 39.5 26.0 41.0 66-67 33.72328543360856 36.0 33.0 39.0 25.5 40.5 68-69 33.38919846140925 36.0 33.0 39.0 25.0 40.0 70-71 32.956378065549956 35.0 32.0 38.5 24.0 40.0 72-73 32.470324469055896 35.0 32.0 37.5 22.5 40.0 74-75 31.977622602909946 35.0 31.0 37.0 21.5 39.0 76-77 30.028514100341397 33.0 29.0 35.0 19.5 37.5 78-79 30.973286603536533 34.0 30.5 36.0 19.5 38.0 80-81 31.01922031566381 34.0 31.0 36.0 20.0 37.5 82-83 30.69420718168292 34.0 31.0 35.0 18.0 37.0 84-85 30.291990993278702 34.0 30.5 35.0 15.5 36.5 86-87 29.712795859390912 34.0 30.0 35.0 7.5 36.0 88-89 29.111452141694777 34.0 29.0 35.0 2.0 36.0 90-91 28.59414792175226 34.0 29.0 35.0 2.0 35.0 92-93 28.301170987067 34.0 29.0 35.0 2.0 35.0 94-95 27.964657461098664 33.5 28.5 35.0 2.0 35.0 96-97 27.364012128828037 33.0 27.0 35.0 2.0 35.0 98-99 26.53627466362108 33.0 25.5 35.0 2.0 35.0 100-101 22.83035625661471 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1697372799662773 1101 2 0.170223445693928 1101 3 0.09623209472172078 1101 4 0.13913135089235595 1101 5 -0.052824057043110884 1101 6 0.047705021378781964 1101 7 0.16583737579597368 1101 8 0.22153170629286478 1101 9 0.24569566806352583 1101 10-11 0.27421688781628717 1101 12-13 0.2559516023271513 1101 14-15 0.37364659956575963 1101 16-17 0.3895058578174755 1101 18-19 0.32218268992279775 1101 20-21 0.36770483799121934 1101 22-23 0.313473587230817 1101 24-25 0.3601221497967515 1101 26-27 0.23733548945962468 1101 28-29 0.3155299574601358 1101 30-31 0.3730446653579378 1101 32-33 0.41245439671763506 1101 34-35 0.4670649361037036 1101 36-37 0.5190888120607013 1101 38-39 0.5771365325811431 1101 40-41 0.5169433296183144 1101 42-43 0.6286288443374701 1101 44-45 0.5684679898955025 1101 46-47 0.5892076656544631 1101 48-49 0.47588119431721765 1101 50-51 0.5301324716401936 1101 52-53 0.3719561112053995 1101 54-55 0.5745735793792974 1101 56-57 0.5507482408576791 1101 58-59 0.5255866769746973 1101 60-61 0.559691670413919 1101 62-63 0.3741883114488047 1101 64-65 0.3800280016001807 1101 66-67 0.41283844080732734 1101 68-69 0.44917104843311506 1101 70-71 0.5471078819787749 1101 72-73 0.41326226396829924 1101 74-75 0.2623253663092484 1101 76-77 0.19208686092238025 1101 78-79 0.20698694207896295 1101 80-81 0.3471926690526139 1101 82-83 0.5081897995174671 1101 84-85 0.46298632144971563 1101 86-87 0.4669213032478723 1101 88-89 0.4736902671750336 1101 90-91 0.44321135529654043 1101 92-93 0.5153918891358629 1101 94-95 0.523303625945303 1101 96-97 0.4930976047378266 1101 98-99 0.4840278377372016 1101 100-101 1.0174588100060191 1102 1 0.29516533627433006 1102 2 0.1827763949032999 1102 3 0.18172712030717975 1102 4 0.10602664515631233 1102 5 -0.036797022420834935 1102 6 -0.003833743245252208 1102 7 0.11160153831632158 1102 8 0.15977680583439735 1102 9 0.19883955472545978 1102 10-11 0.2253714057427203 1102 12-13 0.2250387759164525 1102 14-15 0.34830877056953113 1102 16-17 0.3509158585556804 1102 18-19 0.35231606715147024 1102 20-21 0.33321288056753673 1102 22-23 0.27434328899336435 1102 24-25 0.3322787486007144 1102 26-27 0.29518809026775017 1102 28-29 0.29815050585953884 1102 30-31 0.25032487233026757 1102 32-33 0.27118900153590886 1102 34-35 0.3219605935149801 1102 36-37 0.2609997142598175 1102 38-39 0.3825243069699056 1102 40-41 0.4573505275160983 1102 42-43 0.4509662316729006 1102 44-45 0.39436158592224757 1102 46-47 0.42350752680295045 1102 48-49 0.3853242161939576 1102 50-51 0.42821080497443376 1102 52-53 0.32436846897194016 1102 54-55 0.29754757553953937 1102 56-57 0.3386474570045195 1102 58-59 0.39277655014566903 1102 60-61 0.34501365954441354 1102 62-63 0.22313842399379524 1102 64-65 0.37861304458675704 1102 66-67 0.5250689252931622 1102 68-69 0.46520385875848547 1102 70-71 0.37603742219129543 1102 72-73 0.4461891863433891 1102 74-75 0.49990692781028656 1102 76-77 0.4118160851317434 1102 78-79 0.223982760650415 1102 80-81 0.4365859332471729 1102 82-83 0.5038312193224606 1102 84-85 0.5848470688870684 1102 86-87 0.6995941488339064 1102 88-89 0.5963978770789922 1102 90-91 0.5845675373113295 1102 92-93 0.6062089543892171 1102 94-95 0.6492852472970689 1102 96-97 0.5849653967280481 1102 98-99 0.8284447453138561 1102 100-101 1.368536005707238 1103 1 0.15945725134260869 1103 2 0.14624732431683896 1103 3 0.17667864132236843 1103 4 0.07718256092250897 1103 5 -0.02802797174226157 1103 6 0.06762672594865649 1103 7 0.06829223888931324 1103 8 0.08036694871493921 1103 9 0.1798653899233713 1103 10-11 0.1696515898906341 1103 12-13 0.18100268501236627 1103 14-15 0.1781151406630812 1103 16-17 0.09955979491052602 1103 18-19 -0.042538095448165336 1103 20-21 0.09400203792204564 1103 22-23 0.10166314017049416 1103 24-25 0.15897638904461076 1103 26-27 0.18778447080865845 1103 28-29 0.15606406817643403 1103 30-31 0.14299883926916124 1103 32-33 0.25841477652391376 1103 34-35 0.22015865836005588 1103 36-37 0.24084003287175904 1103 38-39 0.1729811442622875 1103 40-41 0.3441660957095394 1103 42-43 0.365329619187996 1103 44-45 0.29601358625691176 1103 46-47 0.40852781010413963 1103 48-49 0.31159166330112953 1103 50-51 0.33502998790742566 1103 52-53 0.3605002221170466 1103 54-55 0.32374746527389675 1103 56-57 0.22826337573116007 1103 58-59 0.29079155399638523 1103 60-61 0.34772122818692 1103 62-63 0.26819761848251744 1103 64-65 0.2511036430460436 1103 66-67 0.2843689243635552 1103 68-69 0.15212432948267463 1103 70-71 0.23959047877359296 1103 72-73 0.31101142410673077 1103 74-75 0.460809997728866 1103 76-77 0.4027024543099955 1103 78-79 0.27016811991833833 1103 80-81 0.5051964430934923 1103 82-83 0.5363106911056796 1103 84-85 0.5018771759848981 1103 86-87 0.4842567659911552 1103 88-89 0.3590062924501076 1103 90-91 0.3672876341906388 1103 92-93 0.409197335736728 1103 94-95 0.5222567384075276 1103 96-97 0.5475139597349958 1103 98-99 0.6586200083388967 1103 100-101 1.2384231693415977 1104 1 0.1505176539509634 1104 2 0.14802824125248293 1104 3 0.12257926767142635 1104 4 0.10037777383292479 1104 5 -0.020618997699926922 1104 6 0.0351330353342405 1104 7 0.07015997789130068 1104 8 0.031463887562864556 1104 9 0.11028708493613948 1104 10-11 0.12937727001259702 1104 12-13 0.060162100735347224 1104 14-15 0.05764786761406526 1104 16-17 0.14995101466828942 1104 18-19 0.19043697266102555 1104 20-21 0.16150087162017002 1104 22-23 0.12182963508561073 1104 24-25 0.0837241319074522 1104 26-27 0.10419540272406635 1104 28-29 0.11911288063036096 1104 30-31 -0.003304740828099284 1104 32-33 0.11805992740504934 1104 34-35 0.17810448612210195 1104 36-37 0.2246921931088579 1104 38-39 0.212226610825617 1104 40-41 0.28303815641590546 1104 42-43 0.2775279065931713 1104 44-45 0.31546894457417096 1104 46-47 0.31197173629734465 1104 48-49 0.24778312228090016 1104 50-51 0.09825686839767656 1104 52-53 0.1916136784490945 1104 54-55 0.1651793811601081 1104 56-57 0.11130104089416903 1104 58-59 0.20387154669102614 1104 60-61 0.37413701929917664 1104 62-63 0.2423287827309366 1104 64-65 0.1364064888226153 1104 66-67 0.08634612540939202 1104 68-69 0.19084827221701772 1104 70-71 0.062053547458120306 1104 72-73 0.0977453199145728 1104 74-75 0.2347682748762523 1104 76-77 0.21893349658169114 1104 78-79 0.006689408121232532 1104 80-81 0.206151911653496 1104 82-83 0.2276779990727782 1104 84-85 0.2876516524738584 1104 86-87 0.4563161475530819 1104 88-89 0.3273486486543149 1104 90-91 0.3407359322415644 1104 92-93 0.3708055406349118 1104 94-95 0.424301930357629 1104 96-97 0.6205088431473911 1104 98-99 0.9636525417736728 1104 100-101 1.7241365628400196 1105 1 0.09908050990132722 1105 2 0.08481428979929007 1105 3 0.04946008411352665 1105 4 0.03877898606900487 1105 5 0.017397264417674307 1105 6 0.012201749286035124 1105 7 0.05398269983763271 1105 8 0.08684024817923586 1105 9 0.13049237713408957 1105 10-11 0.10624489875244336 1105 12-13 0.08198462168250842 1105 14-15 0.1304060385400021 1105 16-17 0.07022226258631292 1105 18-19 0.11283597803637946 1105 20-21 0.033152061427401236 1105 22-23 0.039575859639768396 1105 24-25 0.05937532561535619 1105 26-27 0.06225985760445951 1105 28-29 0.10756529488046596 1105 30-31 0.09982894174511614 1105 32-33 0.1274111498172843 1105 34-35 0.11451741849116104 1105 36-37 0.09695934440685505 1105 38-39 0.21669178668727085 1105 40-41 0.17127120393729456 1105 42-43 0.23712282902378234 1105 44-45 0.24913027061341353 1105 46-47 0.18865846004991482 1105 48-49 0.1382068255437119 1105 50-51 0.1751075579097261 1105 52-53 0.22507074711486297 1105 54-55 0.29190448060995067 1105 56-57 0.14692524656312855 1105 58-59 0.17591545353663207 1105 60-61 0.146444599881832 1105 62-63 0.1888332328203859 1105 64-65 0.18175701209732864 1105 66-67 0.16479238922263306 1105 68-69 0.1193169763483013 1105 70-71 0.010330048429736394 1105 72-73 0.15156680511945098 1105 74-75 0.25951787488560996 1105 76-77 0.3711290166865453 1105 78-79 0.07131683574259995 1105 80-81 0.24073919909859853 1105 82-83 0.37738188094951397 1105 84-85 0.2778499028174046 1105 86-87 0.32632105436495706 1105 88-89 0.3264405672257915 1105 90-91 0.2406973443020206 1105 92-93 0.4556530600504942 1105 94-95 0.5486157281474178 1105 96-97 0.6160852266170913 1105 98-99 0.7554651871452869 1105 100-101 1.4875811642800123 1106 1 0.0801577712387811 1106 2 0.06663902027073476 1106 3 0.06910581885019695 1106 4 0.037887266531619446 1106 5 -0.08761567901800049 1106 6 0.027798008384742445 1106 7 -0.010696503074242969 1106 8 0.031677408658431716 1106 9 0.05473362929510728 1106 10-11 0.10144584423395031 1106 12-13 0.0642248034425208 1106 14-15 0.08077498257584637 1106 16-17 0.1631765699207932 1106 18-19 0.0037057376439761924 1106 20-21 -0.02564235513681723 1106 22-23 0.12309194859501105 1106 24-25 0.027472582342866758 1106 26-27 0.05575744998984078 1106 28-29 0.14065605049434993 1106 30-31 0.1603902851011867 1106 32-33 0.19504844241723873 1106 34-35 0.22529508963140898 1106 36-37 0.20009728576793862 1106 38-39 0.15973425611014846 1106 40-41 0.22772734621680257 1106 42-43 0.19347228564027574 1106 44-45 0.16850726302288166 1106 46-47 0.15405751973306536 1106 48-49 0.1994952719471783 1106 50-51 0.27755651231090184 1106 52-53 0.2361408403774803 1106 54-55 0.2407389075327515 1106 56-57 0.2971875836998379 1106 58-59 0.3477606384227272 1106 60-61 0.33614192551647903 1106 62-63 0.45666668121461385 1106 64-65 0.5248553307306025 1106 66-67 0.3779521498637308 1106 68-69 0.39030607109657467 1106 70-71 0.3993430623028047 1106 72-73 0.37724937423038085 1106 74-75 0.5041340408212847 1106 76-77 0.41256280718601346 1106 78-79 0.3017728048541102 1106 80-81 0.33180519889032567 1106 82-83 0.45690050655147374 1106 84-85 0.5313468920965754 1106 86-87 0.6660599532584186 1106 88-89 0.6908545271767252 1106 90-91 0.539937126672946 1106 92-93 0.6871149563120582 1106 94-95 0.8798987910403149 1106 96-97 0.9838799569428183 1106 98-99 1.2501119725357377 1106 100-101 1.6154235062404432 1107 1 0.00904832874007866 1107 2 -0.0056601961927853495 1107 3 -0.03672761100079214 1107 4 0.01525325060077165 1107 5 -0.07635596497859609 1107 6 -0.05470132085026336 1107 7 -0.03004335007513248 1107 8 -0.010230463381248 1107 9 -0.029302376854069223 1107 10-11 -0.1120366218625648 1107 12-13 0.01675204861040669 1107 14-15 -0.012251236627122353 1107 16-17 0.0365564047685325 1107 18-19 -0.04052626642916124 1107 20-21 -0.06137977785869708 1107 22-23 -0.007219890695864706 1107 24-25 -0.09130591115426512 1107 26-27 -0.1418056576999831 1107 28-29 -0.005632244917173068 1107 30-31 -0.01911587377161794 1107 32-33 -0.03293126499355026 1107 34-35 -0.03469510424967126 1107 36-37 -0.0539736663124728 1107 38-39 -0.1407939972514427 1107 40-41 -0.16590978769210807 1107 42-43 -0.14295526540541204 1107 44-45 -0.02509934028709182 1107 46-47 -0.09282845447838639 1107 48-49 -0.041474459705334255 1107 50-51 -0.264794098261838 1107 52-53 -0.23468262021825836 1107 54-55 -0.14706543000987438 1107 56-57 -0.28000863768165374 1107 58-59 -0.11463943777483365 1107 60-61 -0.054898593846786525 1107 62-63 -0.1516145223076535 1107 64-65 -0.07117946428552102 1107 66-67 -0.13087487080390048 1107 68-69 -0.17200154997104278 1107 70-71 -0.2596934680129124 1107 72-73 -0.15251955745988965 1107 74-75 -0.12780375187807635 1107 76-77 -0.22497428950987697 1107 78-79 -0.032846483734722653 1107 80-81 0.0227059816195343 1107 82-83 -0.04002008507614718 1107 84-85 -0.10292644096692172 1107 86-87 0.02074714019504853 1107 88-89 -0.10847677646415477 1107 90-91 0.006405183739488507 1107 92-93 0.1833048261404855 1107 94-95 0.1100260115863847 1107 96-97 0.41555428727941646 1107 98-99 0.7877355539084157 1107 100-101 1.422453751266719 1108 1 0.059058704528574424 1108 2 0.03678090933977529 1108 3 -0.03251231591718806 1108 4 -0.05474072120791362 1108 5 -0.10819126379329447 1108 6 -0.009188168135800368 1108 7 -0.08199010816217367 1108 8 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-1.9164167678116435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 40.0 5 101.0 6 256.0 7 611.0 8 1376.0 9 2472.0 10 4146.0 11 6187.0 12 8611.0 13 12003.0 14 14883.0 15 19191.0 16 24819.0 17 33095.0 18 32075.0 19 23186.0 20 21781.0 21 22943.0 22 25104.0 23 28026.0 24 32852.0 25 39221.0 26 46876.0 27 57282.0 28 70010.0 29 85415.0 30 102764.0 31 125308.0 32 154539.0 33 194143.0 34 257205.0 35 360867.0 36 544536.0 37 863117.0 38 765861.0 39 79156.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54564280113682 19.874936552358868 14.727382520643935 22.852038125860368 2 15.623021128406615 21.089491115813264 39.57504275163119 23.712445004148936 3 19.40555836475103 25.091088129073352 29.909023937711552 25.594329568464065 4 12.910216771644098 17.136242915470365 37.2958506472411 32.65768966564443 5 13.321193689487586 38.07488177767354 34.334340672471555 14.269583860367325 6 31.440962309890548 36.89806985298645 17.38164357823768 14.279324258885323 7 28.196076972886488 32.958225689697166 20.862204897983514 17.98349243943283 8 26.07077349972747 35.42480331047745 19.705601787551423 18.79882140224365 9 26.273774475816854 16.891838804121793 20.485192529504967 36.34919419055639 10-11 24.800995251570228 27.058241970168563 27.291057637055832 20.84970514120538 12-13 25.799079278999248 24.27252031302839 27.821207419855625 22.107192988116733 14-15 22.578008950072153 25.772712796967745 26.378008900812027 25.271269352148074 16-17 21.83595264403171 29.051282101806564 26.93884246549362 22.173922788668104 18-19 21.660662267901685 27.758770211121508 29.27189347793479 21.308674043042018 20-21 22.634608833966038 26.66810177831515 29.2714747668703 21.42581462084851 22-23 22.13635498225527 26.7407235179414 29.0926358822082 22.030285617595126 24-25 22.07172919136097 26.8611874817198 29.350188574683273 21.71689475223596 26-27 21.9677834496093 27.02133607551678 29.340361204672384 21.670519270201538 28-29 21.6395419892076 27.26502366086965 29.368837404395332 21.726596945527415 30-31 21.914721363796122 26.963009833059896 29.279836673128774 21.842432130015204 32-33 21.73242195533634 27.120642233808752 29.17376730846863 21.973168502386283 34-35 22.175468690268346 27.053106243029255 29.077886134965457 21.693538931736942 36-37 22.18679023111676 26.87497228668135 29.09841800060107 21.83981948160082 38-39 21.95753464981913 27.161886369038896 29.22687148436586 21.653707496776118 40-41 22.06935971526315 27.020417644097904 29.198668341154633 21.711554299484316 42-43 21.864314347181587 27.0898618294749 28.832930272335894 22.21289355100762 44-45 22.012852190450058 27.12668357253899 28.724555752863257 22.135908484147695 46-47 22.177257023829856 27.284985284060916 28.41923142145787 22.11852627065136 48-49 22.25479195661386 27.312579425673334 28.28691284114939 22.145715776563414 50-51 22.091432468608673 27.492103908828362 28.26163336497065 22.15483025759231 52-53 22.242609072385044 27.599142775300677 28.03075999260113 22.12748815971315 54-55 21.995705009680847 27.369664481898013 28.182062467257413 22.45256804116373 56-57 22.111853593218083 27.667895598321245 27.938004406617146 22.282246401843533 58-59 22.316680229671256 27.368792708686367 28.028819250139897 22.28570781150248 60-61 22.17509826101253 27.465632506584086 28.320575668214907 22.03869356418847 62-63 22.074406345646295 27.410666905416157 28.333243227566403 22.181683521371138 64-65 21.910909666813957 27.38946220382491 28.254151340364835 22.445476788996295 66-67 21.910793808083394 27.599353324507863 27.88791138767363 22.601941479735107 68-69 21.81978926959123 27.445494152931882 28.10861096203045 22.626105615446438 70-71 21.96171523453572 27.3372939338921 28.024177881464986 22.676812950107198 72-73 22.000076845795366 27.25786862767941 27.995231127276092 22.746823399249127 74-75 21.804709338110932 27.634449334628226 27.72813019311779 22.832711134143057 76-77 21.91507572376432 27.338493223639247 27.922665228088068 22.823765824508364 78-79 21.81266352307714 27.3032897929535 28.013082462803222 22.870964221166137 80-81 21.826168086645517 27.51282808506425 27.768256791748446 22.892747036541785 82-83 21.918704189149015 27.42672049540435 27.80006684668145 22.854508468765186 84-85 21.76062337703281 27.397213206935135 27.663008527666577 23.17915488836547 86-87 21.72294566770207 27.421354788983333 27.817138943119218 23.038560600195375 88-89 21.836894106247236 27.294084494508674 27.65354828981552 23.21547310942857 90-91 21.761278645902237 27.27943771153759 27.774912009511603 23.184371633048567 92-93 21.823113451525348 27.396688718885926 27.676918567705894 23.10327926188283 94-95 21.606921757695133 27.202851578557695 27.725269065756798 23.464957597990377 96-97 21.56764442014274 27.09733631603869 27.78622795528898 23.54879130852959 98-99 21.446122107476235 27.194015190344818 27.522838841313185 23.83702386086576 100-101 21.632605107380456 26.68243667867685 28.04235391074418 23.642604303198514 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2477.0 1 1617.0 2 957.0 3 1219.0 4 1027.5 5 985.0 6 1886.0 7 3898.0 8 5854.0 9 6346.5 10 5366.0 11 4204.5 12 4009.0 13 4213.0 14 4441.0 15 4629.0 16 4771.5 17 5038.0 18 5497.5 19 6068.5 20 6898.0 21 8147.5 22 9623.0 23 11612.0 24 14121.0 25 17304.0 26 22208.5 27 28258.5 28 33391.5 29 39488.0 30 47938.5 31 56069.5 32 65089.0 33 74624.0 34 83443.5 35 92609.0 36 104224.5 37 114052.5 38 118174.0 39 119900.0 40 121961.5 41 126097.0 42 129443.0 43 135177.5 44 141987.0 45 145954.0 46 147215.0 47 152851.0 48 171423.0 49 171182.5 50 154985.5 51 146782.0 52 138071.0 53 132762.0 54 128578.5 55 119521.5 56 107679.0 57 95967.5 58 86331.0 59 76277.0 60 64921.5 61 53864.0 62 43029.5 63 33169.0 64 24690.5 65 18308.5 66 13445.5 67 10150.0 68 7320.5 69 4994.5 70 3768.5 71 2865.5 72 2179.0 73 1847.0 74 1515.0 75 1144.5 76 800.5 77 483.5 78 306.5 79 205.0 80 129.5 81 86.5 82 59.0 83 39.5 84 24.5 85 17.5 86 8.5 87 4.5 88 5.5 89 3.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23386244454849275 2 0.0 3 0.0 4 0.0 5 0.029580705203026837 6 0.004458041333678483 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.00258615657478586 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.0639603318063516E-4 26-27 0.0013300233813184424 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006280665967337088 36-37 0.0162312112646084 38-39 0.013152453437482375 40-41 0.008140235694921207 42-43 0.007709209599123565 44-45 0.003657564298625716 46-47 6.280665967337088E-4 48-49 0.0017856795397330938 50-51 1.2315031308504095E-5 52-53 0.0 54-55 0.0 56-57 0.0011945580369248972 58-59 3.694509392551229E-4 60-61 0.002499951355626331 62-63 8.743672229037908E-4 64-65 0.0016009540701055326 66-67 0.005849639871539445 68-69 2.463006261700819E-4 70-71 1.1083528177653687E-4 72-73 0.0 74-75 4.433411271061475E-4 76-77 0.05837324840230942 78-79 0.14276815795948797 80-81 0.0010344626299143439 82-83 0.0010344626299143439 84-85 0.0 86-87 0.10672206131949649 88-89 0.3598205847718727 90-91 0.3984158928927245 92-93 4.6797118972315567E-4 94-95 2.955607514040983E-4 96-97 1.6009540701055326E-4 98-99 0.0 100-101 0.00129307828739293 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4060079.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.69710123283408 #Duplication Level Percentage of deduplicated Percentage of total 1 67.03799498169612 29.964040481430942 2 16.203452726094334 14.484947336393594 3 6.585785044348127 8.830965024747387 4 3.379836123637994 6.042755094745478 5 1.8933289783570346 4.231315850634135 6 1.1496838962990714 3.083252249918323 7 0.8125748611824831 2.5423818580670683 8 0.5431387350742521 1.9421381620069837 9 0.39811311470909033 1.6015051971243988 >10 1.7911432212024827 13.912340544284621 >50 0.12575764424482005 3.923680606822641 >100 0.0734011207356772 6.017240927114569 >500 0.004245671834937668 1.235939832572847 >1k 0.001323326214477976 1.100538211656163 >5k 1.1027718453983127E-4 0.39799779599228235 >10k+ 1.1027718453983127E-4 0.6889608264885908 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15353 0.37814535135892674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12602 0.3103880490995372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8800 0.21674455102967208 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7349 0.1810063301723932 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.389018785102457E-5 2.463006261700819E-5 2 0.0 0.0 0.0 7.389018785102457E-5 2.463006261700819E-5 3 0.0 0.0 0.0 1.2315031308504095E-4 2.463006261700819E-5 4 0.0 0.0 0.0 1.9704050093606552E-4 2.463006261700819E-5 5 2.463006261700819E-5 0.0 0.0 1.9704050093606552E-4 2.463006261700819E-5 6 2.463006261700819E-5 0.0 0.0 1.9704050093606552E-4 2.463006261700819E-5 7 2.463006261700819E-5 0.0 0.0 1.9704050093606552E-4 4.926012523401638E-5 8 2.463006261700819E-5 0.0 0.0 1.9704050093606552E-4 4.926012523401638E-5 9 2.463006261700819E-5 0.0 0.0 2.463006261700819E-4 4.926012523401638E-5 10-11 4.926012523401638E-5 0.0 0.0 3.571359079466188E-4 4.926012523401638E-5 12-13 4.926012523401638E-5 0.0 0.0 4.679711897231556E-4 1.4778037570204914E-4 14-15 4.926012523401638E-5 0.0 0.0 4.679711897231556E-4 2.216705635530737E-4 16-17 4.926012523401638E-5 0.0 0.0 6.650116906592212E-4 2.709306887870901E-4 18-19 6.157515654252048E-5 0.0 0.0 7.881620037442621E-4 2.709306887870901E-4 20-21 7.389018785102457E-5 0.0 0.0 9.852025046803276E-4 2.955607514040983E-4 22-23 8.620521915952867E-5 0.0 0.0 0.00129307828739293 3.078757827126024E-4 24-25 1.2315031308504095E-4 0.0 0.0 0.0016994743205735652 3.2019081402110647E-4 26-27 1.2315031308504095E-4 0.0 0.0 0.002426061167775307 3.4482087663811466E-4 28-29 1.2315031308504095E-4 0.0 0.0 0.004938327554710142 3.4482087663811466E-4 30-31 1.2315031308504095E-4 0.0 0.0 0.013066248218322845 3.571359079466188E-4 32-33 1.2315031308504095E-4 0.0 0.0 0.02834920207217643 3.9408100187213104E-4 34-35 1.3546534439354505E-4 0.0 0.0 0.05248666343684445 4.1871106448913923E-4 36-37 1.4778037570204914E-4 0.0 0.0 0.09360655297593963 4.1871106448913923E-4 38-39 1.4778037570204914E-4 0.0 0.0 0.15561273561425776 4.1871106448913923E-4 40-41 1.7241043831905733E-4 0.0 0.0 0.22439218547225312 4.1871106448913923E-4 42-43 1.7241043831905733E-4 0.0 0.0 0.2943908234297904 4.310260957976433E-4 44-45 1.7241043831905733E-4 0.0 0.0 0.38161819018792487 4.433411271061474E-4 46-47 1.8472546962756143E-4 0.0 0.0 0.4894978644504208 4.679711897231556E-4 48-49 1.9704050093606552E-4 0.0 0.0 0.6046557222162425 4.802862210316597E-4 50-51 1.9704050093606552E-4 0.0 0.0 0.7320177760087919 4.926012523401638E-4 52-53 2.216705635530737E-4 0.0 0.0 0.8560670863793538 5.418613775741803E-4 54-55 2.216705635530737E-4 0.0 0.0 0.9974190157383638 5.788064714996924E-4 56-57 2.216705635530737E-4 0.0 0.0 1.1686348960204962 6.034365341167007E-4 58-59 2.216705635530737E-4 0.0 0.0 1.3584464735784696 6.157515654252048E-4 60-61 2.216705635530737E-4 0.0 0.0 1.5697724108324 6.650116906592212E-4 62-63 2.216705635530737E-4 0.0 0.0 1.7798791599867885 6.896417532762293E-4 64-65 2.216705635530737E-4 0.0 0.0 1.9974980782393643 6.896417532762293E-4 66-67 2.216705635530737E-4 0.0 0.0 2.245510493761328 7.019567845847334E-4 68-69 2.216705635530737E-4 0.0 0.0 2.522697218453138 7.265868472017417E-4 70-71 2.216705635530737E-4 0.0 0.0 2.836459586131206 7.63531941127254E-4 72-73 2.216705635530737E-4 0.0 0.0 3.1523278241630273 8.866822542122948E-4 74-75 2.216705635530737E-4 0.0 0.0 3.472838828998155 9.482574107548153E-4 76-77 2.216705635530737E-4 0.0 0.0 3.8149011386231644 9.605724420633195E-4 78-79 2.463006261700819E-4 1.2315031308504095E-5 0.0 4.207319611268648 9.605724420633195E-4 80-81 2.463006261700819E-4 2.463006261700819E-5 0.0 4.638493487441993 0.0010098325672973358 82-83 2.463006261700819E-4 2.463006261700819E-5 0.0 5.074445595763038 0.0010837227551483604 84-85 2.463006261700819E-4 2.463006261700819E-5 0.0 5.5153360316387925 0.0010837227551483604 86-87 2.463006261700819E-4 2.463006261700819E-5 0.0 5.978639824496026 0.0011206678490738725 88-89 2.463006261700819E-4 2.463006261700819E-5 0.0 6.47938131253111 0.001169927974307889 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 17555 0.0 24.086967 1 GTATCAA 26830 0.0 23.950571 1 TACATGG 17975 0.0 23.180706 2 ACATGGG 18025 0.0 22.221235 3 GGTATCA 19960 0.0 21.70896 1 GAGTACT 14710 0.0 20.625565 12-13 TCAACGC 30995 0.0 20.501703 4 ATCAACG 31120 0.0 20.404104 3 CAACGCA 31285 0.0 20.296492 5 TATCAAC 31635 0.0 20.206951 2 AACGCAG 31850 0.0 20.020912 6 GTACTTT 15075 0.0 19.90587 14-15 AGTACTT 15385 0.0 19.658966 12-13 CATGGGG 7005 0.0 19.443516 4 TACCTGG 2915 0.0 18.722347 2 GTGTAGC 2710 0.0 18.428976 1 CATGGGA 11090 0.0 18.144096 4 AGAGTAC 26740 0.0 18.141779 10-11 ACGCAGA 35150 0.0 18.085953 7 CGCAGAG 35320 0.0 17.91831 8 >>END_MODULE