FastQCFastQC Report
Thu 26 May 2016
SRR938727_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938727_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4352916
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121330.2787326932107121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95410.21918640286189764No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90430.20774579615136152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61930.14227244449467896No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51820.11904663448594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG182550.024.461181
TACATGG186650.023.2787592
ACATGGG181550.022.3928593
GTATCAA275900.021.6656511
GAGTACT119600.021.33911912-13
TACCTGG37250.020.0978362
GTACTTT126900.019.79543914-15
GGTATCA214450.019.4962391
AGTACTT128000.019.08792312-13
ATCAACG315850.018.8120083
CAACGCA316800.018.7406395
TCAACGC317250.018.7140564
TATCAAC319250.018.6413442
AGAGTAC228450.018.54986610-11
AACGCAG321400.018.5467936
CATGGGA85350.018.375664
CATGGGG99500.018.1910924
GTACCTG43050.018.058391
ACTTTTT151550.016.85841816-17
ACGCAGA352350.016.783877
Produced by FastQC (version 0.11.5)