##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938727_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4352916 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.483216078601103 33.0 31.0 34.0 30.0 34.0 2 31.5494787861746 33.0 31.0 34.0 28.0 34.0 3 31.564980578536318 33.0 31.0 34.0 28.0 34.0 4 35.106604630091645 37.0 35.0 37.0 32.0 37.0 5 35.13352474525123 37.0 35.0 37.0 32.0 37.0 6 35.21378335809834 37.0 35.0 37.0 32.0 37.0 7 35.11931404143797 37.0 35.0 37.0 32.0 37.0 8 35.08579237458292 37.0 35.0 37.0 32.0 37.0 9 36.636493559719504 39.0 37.0 39.0 32.0 39.0 10-11 36.52492719822758 39.0 37.0 39.0 32.0 39.0 12-13 36.40315985881648 39.0 37.0 39.0 32.0 39.0 14-15 37.544564838834475 40.0 37.0 41.0 32.0 41.0 16-17 37.4770703133256 40.0 37.0 41.0 32.0 41.0 18-19 37.399479222663615 40.0 37.0 41.0 32.0 41.0 20-21 37.28320440826333 40.0 37.0 41.0 31.0 41.0 22-23 37.19799382758592 40.0 37.0 41.0 31.0 41.0 24-25 37.211547960034146 40.0 37.0 41.0 31.0 41.0 26-27 37.1212308025241 40.0 37.0 41.0 31.0 41.0 28-29 37.0026088258997 40.0 36.5 41.0 30.0 41.0 30-31 36.81434399377336 39.5 36.0 41.0 30.0 41.0 32-33 36.62822749164009 39.0 36.0 41.0 30.0 41.0 34-35 36.30730181790781 39.0 35.5 41.0 29.5 41.0 36-37 36.065503676156396 39.0 35.0 41.0 29.0 41.0 38-39 35.90355442190936 39.0 35.0 41.0 27.5 41.0 40-41 35.7340505766709 39.0 35.0 41.0 27.0 41.0 42-43 35.48546859162915 39.0 35.0 40.0 26.5 41.0 44-45 35.47217451473908 39.0 35.0 41.0 26.0 41.0 46-47 35.31345298186319 39.0 35.0 41.0 25.5 41.0 48-49 35.106494244318064 39.0 35.0 41.0 24.0 41.0 50-51 33.97253025328308 38.0 33.0 40.0 23.0 40.5 52-53 34.07598561516005 38.0 33.5 39.5 23.0 40.5 54-55 34.596451551098156 38.0 34.0 40.0 23.0 41.0 56-57 34.503323863819105 38.0 34.0 40.0 23.0 41.0 58-59 34.28035275663487 38.0 34.0 40.0 23.0 41.0 60-61 33.98142911556299 38.0 34.0 40.0 22.0 41.0 62-63 33.619031701967145 37.0 33.0 40.0 21.5 41.0 64-65 33.21945082331017 37.0 33.0 40.0 20.0 41.0 66-67 32.82204297073502 36.0 32.0 39.0 18.5 41.0 68-69 32.41626142107957 36.0 32.0 39.0 18.0 40.5 70-71 32.011875257873115 35.0 31.5 38.5 14.5 40.0 72-73 31.597314306088148 35.0 31.0 37.5 12.0 40.0 74-75 31.149556527164776 35.0 31.0 37.0 8.0 39.0 76-77 30.717439872490075 35.0 30.5 36.5 7.0 39.0 78-79 30.307809408681443 34.5 30.0 36.0 4.5 38.5 80-81 29.882219987704794 34.0 30.0 35.5 2.0 37.0 82-83 29.44247488350338 34.0 29.0 35.0 2.0 37.0 84-85 29.055594571546983 34.0 29.0 35.0 2.0 36.5 86-87 28.694825147096797 34.0 29.0 35.0 2.0 36.0 88-89 28.34004504107132 34.0 29.0 35.0 2.0 36.0 90-91 27.94922576038683 34.0 27.5 35.0 2.0 35.0 92-93 27.520912073653616 34.0 27.0 35.0 2.0 35.0 94-95 27.03913192903332 33.5 25.5 35.0 2.0 35.0 96-97 26.474220154949002 33.0 25.0 35.0 2.0 35.0 98-99 25.789490768946607 33.0 23.5 35.0 2.0 35.0 100-101 23.56722872667426 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2553204862404641 1101 2 0.11341867231027436 1101 3 0.34478951786730505 1101 4 0.2289240248620672 1101 5 0.03880131738142012 1101 6 0.04097770936201073 1101 7 0.11692060147316141 1101 8 0.20507094289862948 1101 9 0.4393780136118508 1101 10-11 0.41222200126190245 1101 12-13 0.32238712177895934 1101 14-15 0.4348972384231544 1101 16-17 0.48750681639715765 1101 18-19 0.41129138604284066 1101 20-21 0.5107952965647868 1101 22-23 0.47160388655026253 1101 24-25 0.4258679871117508 1101 26-27 0.46625642090567965 1101 28-29 0.4404298185659954 1101 30-31 0.4293188077860819 1101 32-33 0.3789017950301954 1101 34-35 0.3968250702342573 1101 36-37 0.4717851814793548 1101 38-39 0.4320588303015498 1101 40-41 0.36106925204254026 1101 42-43 0.3953573607652885 1101 44-45 0.5212087318868441 1101 46-47 0.4509925929866654 1101 48-49 0.4488248728473607 1101 50-51 0.3406662132080953 1101 52-53 0.40904514361596256 1101 54-55 0.5203364462085958 1101 56-57 0.5725556408297336 1101 58-59 0.4757785451842409 1101 60-61 0.2680314858895443 1101 62-63 0.25070574017015446 1101 64-65 0.17139796441234267 1101 66-67 0.06998463983911307 1101 68-69 0.13615419589552147 1101 70-71 0.07393671013791447 1101 72-73 -0.0012590077769480956 1101 74-75 -0.013072709791270398 1101 76-77 -0.09473500166823001 1101 78-79 -0.20136156388917925 1101 80-81 -0.22498391079969338 1101 82-83 -0.3710439756335582 1101 84-85 -0.32781747123025795 1101 86-87 -0.2611882205902454 1101 88-89 -0.2989372654167326 1101 90-91 -0.4407396802170105 1101 92-93 -0.5650411542111691 1101 94-95 -0.7061450813818055 1101 96-97 -0.991981474260001 1101 98-99 -0.9720128463749802 1101 100-101 -0.6087370151662022 1102 1 0.3560223223535175 1102 2 0.3808794863101639 1102 3 0.37040418871573877 1102 4 0.3579566032746939 1102 5 0.29095652481759515 1102 6 0.3297847595016705 1102 7 0.3369131353236199 1102 8 0.30097756095858585 1102 9 0.4644264036500019 1102 10-11 0.40459628195662845 1102 12-13 0.38718350444304406 1102 14-15 0.28601865047335906 1102 16-17 0.44582107809698357 1102 18-19 0.42425479288208123 1102 20-21 0.37559343968735703 1102 22-23 0.468667419239722 1102 24-25 0.46422905443709794 1102 26-27 0.3705712143542428 1102 28-29 0.489812597309772 1102 30-31 0.39638096822072555 1102 32-33 0.420434847412416 1102 34-35 0.4993362288326111 1102 36-37 0.497925732873 1102 38-39 0.44384430985946466 1102 40-41 0.5576239896620052 1102 42-43 0.4844953545287467 1102 44-45 0.6629944777705319 1102 46-47 0.6141349572011521 1102 48-49 0.4929965816476667 1102 50-51 0.4901408743424156 1102 52-53 0.46305488120833616 1102 54-55 0.47246687180620484 1102 56-57 0.4076399532488253 1102 58-59 0.34740241465391364 1102 60-61 0.3689101577996752 1102 62-63 0.31004402445440604 1102 64-65 0.29957243216706075 1102 66-67 0.23985534093493044 1102 68-69 0.2296629792291256 1102 70-71 0.1910684763923669 1102 72-73 0.289868687755817 1102 74-75 0.1611472265433278 1102 76-77 0.27237419460573875 1102 78-79 0.14131528996387743 1102 80-81 0.09304460591924624 1102 82-83 0.06600801025388492 1102 84-85 -0.00897417821593649 1102 86-87 -0.1619184497387387 1102 88-89 -0.1114755215336487 1102 90-91 -0.22736082774704158 1102 92-93 -0.2634584794698682 1102 94-95 -0.337014291956109 1102 96-97 -0.43382574410307484 1102 98-99 -0.35176461721565033 1102 100-101 -0.2102901909430166 1103 1 0.18329523175769324 1103 2 0.36104236646402654 1103 3 0.21226819480621373 1103 4 0.34440729847042917 1103 5 0.21219004647484496 1103 6 0.2959716008688247 1103 7 0.24030196410912907 1103 8 0.21967171490337023 1103 9 0.28830647737681403 1103 10-11 0.3150282248984908 1103 12-13 0.3830714316778838 1103 14-15 0.4241522402477713 1103 16-17 0.5049582941085191 1103 18-19 0.524225242054662 1103 20-21 0.39895762608797014 1103 22-23 0.5505808102723009 1103 24-25 0.4712411151815843 1103 26-27 0.4016075969322799 1103 28-29 0.4548790115422534 1103 30-31 0.4688691478897553 1103 32-33 0.4232096200213036 1103 34-35 0.5439885307919852 1103 36-37 0.5498510444088467 1103 38-39 0.48860279445296584 1103 40-41 0.5925360383858091 1103 42-43 0.4976224331038992 1103 44-45 0.6030543808037478 1103 46-47 0.5480302571170057 1103 48-49 0.580211591464554 1103 50-51 0.6039366029956952 1103 52-53 0.5671549332979353 1103 54-55 0.5430212252942042 1103 56-57 0.5186468851717265 1103 58-59 0.4682918444731996 1103 60-61 0.41779473927981314 1103 62-63 0.4610547629245758 1103 64-65 0.29845450933979834 1103 66-67 0.3505477318472998 1103 68-69 0.2886584514413286 1103 70-71 0.3196701713076209 1103 72-73 0.34112685179797 1103 74-75 0.2677565946564826 1103 76-77 0.3666057729204404 1103 78-79 0.3992048600243798 1103 80-81 0.3452168421655877 1103 82-83 0.40014121937357316 1103 84-85 0.3583150592725417 1103 86-87 0.37564916656129554 1103 88-89 0.15031175257782436 1103 90-91 0.1453842938621257 1103 92-93 0.02242974828186206 1103 94-95 0.04972439859162847 1103 96-97 0.07563903531752914 1103 98-99 0.05550438546897141 1103 100-101 0.10990153883220088 1104 1 0.14703372016671068 1104 2 0.1175922853095166 1104 3 0.10851843738763378 1104 4 0.1528952223263289 1104 5 0.1777632222625769 1104 6 0.12149270986285643 1104 7 0.11337730107677402 1104 8 0.1065454137848505 1104 9 0.1480848241182855 1104 10-11 0.07657383241057403 1104 12-13 0.17299096080530063 1104 14-15 0.21698616459764963 1104 16-17 0.2714712523668794 1104 18-19 0.26656667099153 1104 20-21 0.2664747060251571 1104 22-23 0.3137551866175201 1104 24-25 0.2021871716898218 1104 26-27 0.200064944502941 1104 28-29 0.21532258269358806 1104 30-31 0.12756877916600473 1104 32-33 0.20477497586619364 1104 34-35 0.15662051713685798 1104 36-37 0.37137408082251966 1104 38-39 0.3934305506189375 1104 40-41 0.27926165233732547 1104 42-43 0.30770869285126423 1104 44-45 0.32436036197942997 1104 46-47 0.39500813369158294 1104 48-49 0.4589768410146746 1104 50-51 0.5251968796267263 1104 52-53 0.4662067156097791 1104 54-55 0.41863710643813334 1104 56-57 0.42025431786641576 1104 58-59 0.30545730264334736 1104 60-61 0.40539088973322635 1104 62-63 0.3569447169664244 1104 64-65 0.3199283678839535 1104 66-67 0.3053073446873569 1104 68-69 0.3430539164919679 1104 70-71 0.25546774593448873 1104 72-73 0.1743670118134979 1104 74-75 0.09136197202495921 1104 76-77 0.11245785118161677 1104 78-79 0.14292293851490712 1104 80-81 0.05367708968684681 1104 82-83 0.024997710411291507 1104 84-85 0.09514922840912732 1104 86-87 0.11197975872459054 1104 88-89 0.09825179527215155 1104 90-91 0.01427636938874599 1104 92-93 0.07954929240521835 1104 94-95 0.17530449897623157 1104 96-97 0.15204126618446878 1104 98-99 0.25578467727236287 1104 100-101 0.43853028120190984 1105 1 0.10812386997632117 1105 2 0.1188751517038007 1105 3 0.12586058489034357 1105 4 0.23641008516298712 1105 5 0.09010748905709676 1105 6 0.1778789004184631 1105 7 0.13411378972293164 1105 8 0.1429538047907144 1105 9 0.06676177803584693 1105 10-11 0.13081315430667928 1105 12-13 0.19213016353467083 1105 14-15 0.15861791506319634 1105 16-17 0.1283782328276999 1105 18-19 0.06426875936252685 1105 20-21 0.1059515970588194 1105 22-23 0.20241593316411155 1105 24-25 0.15550868389138373 1105 26-27 0.08966873247647555 1105 28-29 0.1381794922797397 1105 30-31 0.13509139652612845 1105 32-33 0.08260909437976238 1105 34-35 0.1724791603731859 1105 36-37 0.16776181741887086 1105 38-39 0.27107684255312847 1105 40-41 0.07656224782358834 1105 42-43 0.09644287633329185 1105 44-45 0.20131484410789824 1105 46-47 0.18377206542953672 1105 48-49 0.12091744351891265 1105 50-51 0.20100776931237618 1105 52-53 0.2115227767148653 1105 54-55 0.32682291447893164 1105 56-57 0.19360561306638857 1105 58-59 0.2720689024654632 1105 60-61 0.2880012318772529 1105 62-63 0.24531688371647675 1105 64-65 0.1879438248171681 1105 66-67 0.1733725268074764 1105 68-69 0.16945916330260502 1105 70-71 0.13237966851432503 1105 72-73 0.18197170830311293 1105 74-75 0.08156459021890328 1105 76-77 0.0745702987204595 1105 78-79 0.060466201598931235 1105 80-81 -0.003596337173263464 1105 82-83 0.007809081280409913 1105 84-85 -0.08416565839984003 1105 86-87 0.06935962788438133 1105 88-89 0.004300288837843169 1105 90-91 0.08171001992100457 1105 92-93 0.10576423723414763 1105 94-95 0.21107406247160299 1105 96-97 0.2482592857236483 1105 98-99 0.38345550608917023 1105 100-101 0.6029245719687459 1106 1 0.18567686648805193 1106 2 0.2686954405736053 1106 3 0.28957491619886966 1106 4 0.29293346296244493 1106 5 0.24864563815570762 1106 6 0.22687564481651634 1106 7 0.218971564721393 1106 8 0.3103130653089252 1106 9 0.23974330915478248 1106 10-11 0.3849289846278907 1106 12-13 0.3360772435693704 1106 14-15 0.3710589699854765 1106 16-17 0.36865840358247226 1106 18-19 0.410311070943699 1106 20-21 0.3557997475326644 1106 22-23 0.4127553668589101 1106 24-25 0.49435060339913406 1106 26-27 0.2571446506228199 1106 28-29 0.37398721066843876 1106 30-31 0.34626678645884823 1106 32-33 0.37097204729030153 1106 34-35 0.3731299564128392 1106 36-37 0.44862300370693475 1106 38-39 0.4697378097540579 1106 40-41 0.5194689342625907 1106 42-43 0.46055463282741016 1106 44-45 0.5022846592979917 1106 46-47 0.5002257751637416 1106 48-49 0.5196347285683132 1106 50-51 0.6284279664129571 1106 52-53 0.4050362666180902 1106 54-55 0.37022187664769746 1106 56-57 0.5036759665898174 1106 58-59 0.37382532955477643 1106 60-61 0.5036402086287524 1106 62-63 0.3682964038698131 1106 64-65 0.35391870850551044 1106 66-67 0.3079996462138652 1106 68-69 0.509312187665131 1106 70-71 0.5162331067465473 1106 72-73 0.4376640383505723 1106 74-75 0.4084308311663172 1106 76-77 0.17267125524813665 1106 78-79 0.46366102048660807 1106 80-81 0.3414197873111249 1106 82-83 0.3921260687069008 1106 84-85 0.4508139530037596 1106 86-87 0.5250449736104521 1106 88-89 0.5624563328490133 1106 90-91 0.5963568770628704 1106 92-93 0.6613000255023884 1106 94-95 0.7016337141983087 1106 96-97 0.6680464628555498 1106 98-99 0.764450778373913 1106 100-101 0.9582493277769792 1107 1 0.23672269525468437 1107 2 0.1543362680598399 1107 3 0.19394773598383352 1107 4 0.2062706886375807 1107 5 0.2563600797599008 1107 6 0.24306203707769214 1107 7 0.036231640032575285 1107 8 0.37092021539991293 1107 9 0.287665103461336 1107 10-11 0.21355161607116457 1107 12-13 0.32568565748540124 1107 14-15 0.31819671799348725 1107 16-17 0.30561980556475987 1107 18-19 0.40910024604162487 1107 20-21 0.4020904597940458 1107 22-23 0.4774116641202468 1107 24-25 0.3931282703498482 1107 26-27 0.3581148878032394 1107 28-29 0.2885540343422761 1107 30-31 0.2838390442936003 1107 32-33 0.3795950855282584 1107 34-35 0.4141937553079913 1107 36-37 0.5415074530933381 1107 38-39 0.6345785029024569 1107 40-41 0.5317995395932513 1107 42-43 0.5140651540992138 1107 44-45 0.5650046191835756 1107 46-47 0.5446919123742049 1107 48-49 0.6255595762778725 1107 50-51 0.7143577668212089 1107 52-53 0.7190969761041259 1107 54-55 0.5976655098276566 1107 56-57 0.585328607691018 1107 58-59 0.6280159520557902 1107 60-61 0.551028682361661 1107 62-63 0.5441011430597129 1107 64-65 0.5032414325344021 1107 66-67 0.5555412202586183 1107 68-69 0.5115778109972524 1107 70-71 0.539873692917503 1107 72-73 0.4898807029337 1107 74-75 0.46085625683234355 1107 76-77 0.3827808943808968 1107 78-79 0.342059628080456 1107 80-81 0.3534294178300321 1107 82-83 0.46283787124387743 1107 84-85 0.4816795260547906 1107 86-87 0.43645188948116953 1107 88-89 0.35107216774093075 1107 90-91 0.46398400225972125 1107 92-93 0.5348759918601509 1107 94-95 0.5351293848089043 1107 96-97 0.6273005851567248 1107 98-99 0.703397295919352 1107 100-101 0.7772485300133063 1108 1 0.06203554136718026 1108 2 0.016962594659474917 1108 3 0.02125164634285781 1108 4 -0.031093354934924378 1108 5 0.08728148297377203 1108 6 0.10868067045716145 1108 7 0.07523379259760077 1108 8 0.050870680188360495 1108 9 -0.03399705154878063 1108 10-11 0.07329400389988905 1108 12-13 0.13715031796388644 1108 14-15 0.027790648139081497 1108 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5914.0 5 6633.0 6 7270.0 7 8541.0 8 10273.0 9 12328.0 10 15017.0 11 18454.0 12 21817.0 13 26602.0 14 28529.0 15 26660.0 16 31397.0 17 37720.0 18 33381.0 19 27261.0 20 27978.0 21 30408.0 22 33413.0 23 37338.0 24 42323.0 25 48899.0 26 56764.0 27 65827.0 28 77484.0 29 91486.0 30 108884.0 31 131898.0 32 161487.0 33 203760.0 34 270032.0 35 373783.0 36 557674.0 37 833442.0 38 742360.0 39 116289.0 40 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.876056719166854 18.617724578566428 14.359806174383227 23.146412527883488 2 15.318624153858854 20.028615362752152 40.42586852707916 24.22689195630983 3 19.793705675450774 24.118694080908636 29.26421047088902 26.82338977275157 4 12.943195091275081 16.236935528715676 37.116908318325684 33.70296106168355 5 14.1740069714302 37.0717094909255 33.7938629884406 14.960420549203695 6 32.35820142251329 35.4071041089619 17.560533116135147 14.674161352389655 7 29.21556815901783 32.12054950932071 20.417057284873465 18.246825046787997 8 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28.35832921275451 22.712239693782948 32-33 22.494432685574562 26.418186939713028 28.397630542705844 22.689749832006566 34-35 23.15714548401502 26.424720987003436 28.04672237786312 22.371411151118426 36-37 22.969699914624726 26.14828705251705 28.22351678494976 22.658496247908467 38-39 22.694744771357676 26.52747852785655 28.37848160743826 22.39929509334751 40-41 22.92195219193367 26.35808197382471 28.24308217224821 22.47688366199341 42-43 22.686535403694165 26.416776112740862 27.894641425580456 23.002047057984512 44-45 22.8649857642251 26.45888705900547 27.776403121137385 22.899724055632046 46-47 22.94562755524523 26.687986704790372 27.553306346673743 22.81307939329065 48-49 23.113132137454674 26.687990174718486 27.43220446096869 22.766673226858156 50-51 22.676767723400417 26.841762781286167 27.59875341458314 22.88271608073028 52-53 23.02970674385596 26.950942452800025 27.216555746299832 22.802795057044182 54-55 22.7800115459874 26.506754818118385 27.478653657541212 23.234579978353004 56-57 22.892246078869054 26.889988635473316 27.350777556784845 22.866987728872786 58-59 23.167088643517946 26.563118811738413 27.3137942304207 22.955998314322937 60-61 23.045624619137488 26.41068477061986 27.845553301838706 22.698137308403954 62-63 22.885241111817713 26.5307641418675 27.744346039778595 22.83964870653619 64-65 22.742921116293587 26.659851101134034 27.501349491210647 23.095878291361732 66-67 22.751797772161673 26.82433527567687 27.15674938596609 23.267117566195374 68-69 22.574358865417558 26.66908119918138 27.505962633011915 23.25059730238914 70-71 22.72539088133657 26.553446502699007 27.41982297255922 23.301339643405203 72-73 22.819868551772256 26.42477859352197 27.546073488087952 23.20927936661782 74-75 22.602623583532576 26.909501801242016 27.235524931985207 23.2523496832402 76-77 22.79317790736994 26.59187354800289 27.283557939661552 23.331390604965613 78-79 22.77778720039118 26.656804074907235 27.32262132182036 23.24278740288122 80-81 22.747196753313137 26.71111183932537 27.284799801720588 23.2568916056409 82-83 22.805919421467337 26.684179775244594 27.35171124973732 23.15818955355075 84-85 22.751453656771293 26.613308604512582 27.214885020676842 23.420352718039283 86-87 22.742678369688015 26.509875448525992 27.480957084728498 23.266489097057498 88-89 22.81573445912941 26.507906058079335 27.17144197376282 23.504917509028438 90-91 22.777821256095525 26.497122004699815 27.399824224471857 23.325232514732804 92-93 23.05825514486707 26.511214340189692 27.262592389903485 23.167938125039754 94-95 22.889417765203465 26.382914275747215 27.17479349370545 23.55287446534387 96-97 22.873031700805825 26.242727136930572 27.34424511946762 23.539996042795984 98-99 22.7836713143798 26.34243679946237 27.023000702853437 23.850891183304384 100-101 22.970613378594123 25.866168354770924 27.511026951153177 23.652191315481772 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2644.0 1 1523.0 2 481.0 3 583.5 4 555.0 5 572.0 6 1015.5 7 2132.5 8 3431.5 9 4058.0 10 3904.5 11 3736.5 12 3971.5 13 4348.5 14 4805.0 15 5191.5 16 5509.5 17 5926.0 18 6115.0 19 6273.5 20 6737.0 21 7351.0 22 8347.5 23 9685.5 24 11275.0 25 14004.0 26 18390.0 27 24174.5 28 29088.0 29 34136.0 30 40817.5 31 49113.0 32 57735.0 33 65947.5 34 76100.0 35 84818.0 36 94210.5 37 105063.5 38 111225.0 39 114279.0 40 118807.0 41 126863.0 42 135281.0 43 143598.0 44 151840.5 45 157731.5 46 160021.5 47 167800.5 48 190142.5 49 193588.0 50 176973.0 51 168285.5 52 162024.5 53 159361.5 54 155882.5 55 144101.0 56 130370.5 57 116988.5 58 105944.0 59 94589.0 60 80610.0 61 65871.0 62 51763.5 63 40800.5 64 30783.5 65 22858.5 66 17384.5 67 13569.0 68 10452.5 69 7522.5 70 5557.5 71 4253.0 72 3270.5 73 2655.0 74 2093.5 75 1609.5 76 1176.5 77 722.5 78 450.0 79 328.0 80 257.0 81 176.0 82 125.0 83 100.0 84 69.5 85 49.5 86 41.5 87 32.5 88 27.0 89 31.5 90 30.5 91 21.5 92 18.0 93 15.0 94 9.5 95 10.0 96 9.0 97 4.5 98 3.5 99 4.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16434959921119544 2 0.09655596386422344 3 0.08929646241737722 4 0.10514790545004774 5 0.12538721169900818 6 0.04006509659272083 7 0.04380971284536619 8 0.04817460295581169 9 0.04849622643763399 10-11 0.046451620017477935 12-13 0.05085096978669012 14-15 0.048817849919456285 16-17 0.04764622152138934 18-19 0.05213746371397932 20-21 0.05390639286400197 22-23 0.06701254974826071 24-25 0.08381737667347589 26-27 0.0858390099877875 28-29 0.07586868205129618 30-31 0.0643017232586156 32-33 0.052826656889312824 34-35 0.30218823427789554 36-37 0.4793453399973719 38-39 0.4562458820707774 40-41 0.43079168079512675 42-43 0.462046591296501 44-45 0.4714311050339588 46-47 0.4716034033277922 48-49 0.48779944294812955 50-51 0.4769676235424713 52-53 0.4836872570019729 54-55 0.4749689633340042 56-57 0.45246680615936535 58-59 0.43080316734804897 60-61 0.3988590636713412 62-63 0.45848575989061124 64-65 0.49745963395572074 66-67 0.4990218051531433 68-69 0.5098880842175681 70-71 0.5068326611402563 72-73 0.4968853063096095 74-75 0.4898210762624411 76-77 0.4714311050339588 78-79 0.44497757365407464 80-81 0.45112287946746504 82-83 0.46317227348287904 84-85 0.46061077218122287 86-87 0.4716493495394812 88-89 0.48353793181398397 90-91 0.48829336472378515 92-93 0.49028053837933006 94-95 0.4941629932670421 96-97 0.4956447585940092 98-99 0.4890399906637298 100-101 0.4753020733687487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4352916.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.18069282326172 #Duplication Level Percentage of deduplicated Percentage of total 1 72.26945166836863 33.374533480024915 2 14.021458599556414 12.950413450403925 3 5.469764691957692 7.577925691644628 4 2.6258424572475536 4.850528956817121 5 1.4383896138997732 3.3212914459837726 6 0.9054418972628594 2.5088360476084444 7 0.617001811743765 1.9945499797674338 8 0.42934744221812504 1.5862049874822672 9 0.3137949531687236 1.3042141507597158 >10 1.6660831635670175 14.176878167781773 >50 0.1444811554566324 4.58226166939223 >100 0.09200669683000393 7.88737172909805 >500 0.004690430203799169 1.4852348626811231 >1k 0.001995927572408595 1.429815553989579 >5k 1.9959275724085345E-4 0.690355109380859 >10k+ 4.989818931021336E-5 0.2795847171840836 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12133 0.2787326932107121 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9541 0.21918640286189764 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9043 0.20774579615136152 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6193 0.14227244449467896 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5182 0.11904663448594 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.297310584445002E-5 2 0.0 2.297310584445002E-5 0.0 0.0 2.297310584445002E-5 3 0.0 2.297310584445002E-5 0.0 0.0 2.297310584445002E-5 4 0.0 2.297310584445002E-5 0.0 2.297310584445002E-5 2.297310584445002E-5 5 0.0 2.297310584445002E-5 0.0 2.297310584445002E-5 2.297310584445002E-5 6 0.0 2.297310584445002E-5 0.0 2.297310584445002E-5 1.3783863506670012E-4 7 0.0 2.297310584445002E-5 0.0 2.297310584445002E-5 1.3783863506670012E-4 8 2.297310584445002E-5 2.297310584445002E-5 0.0 2.297310584445002E-5 1.3783863506670012E-4 9 2.297310584445002E-5 2.297310584445002E-5 0.0 2.297310584445002E-5 1.3783863506670012E-4 10-11 2.297310584445002E-5 2.297310584445002E-5 0.0 2.297310584445002E-5 1.3783863506670012E-4 12-13 2.297310584445002E-5 2.297310584445002E-5 0.0 1.148655292222501E-4 1.3783863506670012E-4 14-15 2.297310584445002E-5 2.297310584445002E-5 0.0 1.6081174091115013E-4 1.3783863506670012E-4 16-17 2.297310584445002E-5 2.297310584445002E-5 0.0 1.7229829383337515E-4 1.3783863506670012E-4 18-19 2.297310584445002E-5 2.297310584445002E-5 0.0 1.8378484675560017E-4 1.493251879889251E-4 20-21 2.297310584445002E-5 2.297310584445002E-5 0.0 2.527041642889502E-4 1.7229829383337515E-4 22-23 2.297310584445002E-5 2.297310584445002E-5 0.0 3.675696935112003E-4 2.2973105844450022E-4 24-25 6.891931753335006E-5 2.297310584445002E-5 0.0 7.121662811779506E-4 2.871638230556253E-4 26-27 6.891931753335006E-5 2.297310584445002E-5 0.0 0.001297980480211426 2.986503759778503E-4 28-29 6.891931753335006E-5 2.297310584445002E-5 0.0 0.0031243423948452025 2.986503759778503E-4 30-31 6.891931753335006E-5 2.297310584445002E-5 0.0 0.009981814489413533 2.986503759778503E-4 32-33 6.891931753335006E-5 2.297310584445002E-5 0.0 0.024753521547394894 2.986503759778503E-4 34-35 8.040587045557508E-5 2.297310584445002E-5 0.0 0.04601513100643339 3.2162348182230026E-4 36-37 9.189242337780008E-5 2.297310584445002E-5 0.0 0.08200250131176434 3.3311003474452525E-4 38-39 9.189242337780008E-5 2.297310584445002E-5 0.0 0.13678187219785543 3.5608314058897534E-4 40-41 9.189242337780008E-5 2.297310584445002E-5 0.0 0.19489234343139172 3.905427993556503E-4 42-43 9.189242337780008E-5 2.297310584445002E-5 0.0 0.2566555384941956 4.020293522778753E-4 44-45 9.189242337780008E-5 2.297310584445002E-5 0.0 0.33228300293412505 4.1351590520010036E-4 46-47 1.7229829383337515E-4 2.297310584445002E-5 0.0 0.42673692761358134 4.2500245812232535E-4 48-49 1.8378484675560017E-4 2.297310584445002E-5 0.0 0.5304605005012731 4.3648901104455034E-4 50-51 1.8378484675560017E-4 2.297310584445002E-5 0.0 0.6415929000238001 4.3648901104455034E-4 52-53 1.8378484675560017E-4 2.297310584445002E-5 0.0 0.7536327372271829 4.479755639667754E-4 54-55 1.8378484675560017E-4 2.297310584445002E-5 0.0 0.8816733426512251 4.7094866981122537E-4 56-57 2.0675795260005018E-4 2.297310584445002E-5 0.0 1.0371897826652294 4.824352227334504E-4 58-59 2.0675795260005018E-4 2.297310584445002E-5 0.0 1.2092238857813933 4.824352227334504E-4 60-61 2.0675795260005018E-4 2.297310584445002E-5 0.0 1.3930546787486824 4.824352227334504E-4 62-63 2.0675795260005018E-4 2.297310584445002E-5 0.0 1.582008474319284 5.054083285779004E-4 64-65 2.0675795260005018E-4 2.297310584445002E-5 0.0 1.7745460744016195 5.054083285779004E-4 66-67 2.297310584445002E-4 2.297310584445002E-5 0.0 1.9951912694846399 5.054083285779004E-4 68-69 2.527041642889502E-4 2.297310584445002E-5 0.0 2.252501541495402 5.283814344223505E-4 70-71 2.527041642889502E-4 2.297310584445002E-5 0.0 2.5364031835211156 5.743276461112506E-4 72-73 2.8716382305562523E-4 2.297310584445002E-5 0.0 2.820270365888062 6.662200694890506E-4 74-75 3.2162348182230026E-4 2.297310584445002E-5 0.0 3.1103517733859327 6.662200694890506E-4 76-77 3.2162348182230026E-4 2.297310584445002E-5 0.0 3.4336637784877997 6.777066224112757E-4 78-79 3.2162348182230026E-4 2.297310584445002E-5 0.0 3.8036801077714344 6.891931753335006E-4 80-81 4.020293522778753E-4 2.297310584445002E-5 0.0 4.20597365076652 7.121662811779507E-4 82-83 4.1351590520010036E-4 2.297310584445002E-5 0.0 4.613240871176931 7.351393870224007E-4 84-85 4.2500245812232535E-4 2.297310584445002E-5 0.0 5.012214800377494 7.351393870224007E-4 86-87 4.479755639667754E-4 2.297310584445002E-5 0.0 5.437642260957942 7.351393870224007E-4 88-89 4.594621168890004E-4 2.297310584445002E-5 0.0 5.9037091457772215 7.581124928668507E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18255 0.0 24.46118 1 TACATGG 18665 0.0 23.278759 2 ACATGGG 18155 0.0 22.392859 3 GTATCAA 27590 0.0 21.665651 1 GAGTACT 11960 0.0 21.339119 12-13 TACCTGG 3725 0.0 20.097836 2 GTACTTT 12690 0.0 19.795439 14-15 GGTATCA 21445 0.0 19.496239 1 AGTACTT 12800 0.0 19.087923 12-13 ATCAACG 31585 0.0 18.812008 3 CAACGCA 31680 0.0 18.740639 5 TCAACGC 31725 0.0 18.714056 4 TATCAAC 31925 0.0 18.641344 2 AGAGTAC 22845 0.0 18.549866 10-11 AACGCAG 32140 0.0 18.546793 6 CATGGGA 8535 0.0 18.37566 4 CATGGGG 9950 0.0 18.191092 4 GTACCTG 4305 0.0 18.05839 1 ACTTTTT 15155 0.0 16.858418 16-17 ACGCAGA 35235 0.0 16.78387 7 >>END_MODULE