FastQCFastQC Report
Thu 26 May 2016
SRR938727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4352916
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117410.2697272357196877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100270.23035133230230034No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT99050.22754861338927743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64700.1486359948135916No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47200.1084330595858041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG181750.021.9648931
GTATCAA284550.021.0946061
TACATGG189550.020.8037822
GAGTACT128050.020.62412812-13
ACATGGG185800.020.1765293
GGTATCA223900.018.9363121
GTACTTT139700.018.71738614-15
AGTACTT138050.018.51140212-13
AGAGTAC245800.018.15774710-11
ATCAACG328950.018.1330343
TCAACGC329650.018.1233184
CAACGCA330600.018.0855945
TATCAAC330250.018.0760232
AACGCAG335800.017.8337946
GTACACG5650.017.7064021
TACCTGG33000.017.6870842
ACTTTTT160250.016.50923216-17
ACGCAGA369250.016.0768857
AGTACAT142250.016.0456712
CGCAGAG370700.015.9371948
Produced by FastQC (version 0.11.5)