##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938727_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4352916 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00934385134011 33.0 31.0 34.0 30.0 34.0 2 32.2395846830033 34.0 31.0 34.0 30.0 34.0 3 32.33252008538644 34.0 31.0 34.0 30.0 34.0 4 35.953493933721674 37.0 35.0 37.0 35.0 37.0 5 35.85830486965519 37.0 35.0 37.0 35.0 37.0 6 35.885788974563255 37.0 35.0 37.0 35.0 37.0 7 35.82195475400858 37.0 35.0 37.0 33.0 37.0 8 35.7869428217774 37.0 35.0 37.0 33.0 37.0 9 37.44554776614113 39.0 37.0 39.0 34.0 39.0 10-11 37.370677725000895 39.0 37.0 39.0 34.0 39.0 12-13 37.29745301770124 39.0 37.0 39.0 34.0 39.0 14-15 38.53053125766728 40.0 38.0 41.0 34.0 41.0 16-17 38.46079157052422 40.0 38.0 41.0 33.5 41.0 18-19 38.47475347560118 40.0 38.0 41.0 33.5 41.0 20-21 38.55438802402803 40.0 38.0 41.0 34.0 41.0 22-23 38.514709335075615 40.0 38.0 41.0 34.0 41.0 24-25 38.44643854832025 40.0 38.0 41.0 34.0 41.0 26-27 38.29247911055486 40.0 38.0 41.0 33.5 41.0 28-29 38.1364805799147 40.0 38.0 41.0 33.0 41.0 30-31 37.93813939896842 40.0 37.0 41.0 33.0 41.0 32-33 37.74530992557632 40.0 37.0 41.0 33.0 41.0 34-35 37.73103616058752 40.0 37.0 41.0 33.0 41.0 36-37 37.67088935325194 40.0 37.0 41.0 32.5 41.0 38-39 37.58590781443979 40.0 37.0 41.0 32.0 41.0 40-41 37.44759501446846 40.0 37.0 41.0 32.0 41.0 42-43 37.28741928399262 40.0 36.5 41.0 31.0 41.0 44-45 37.06292356663901 40.0 36.0 41.0 31.0 41.0 46-47 36.80697537007376 40.0 36.0 41.0 30.5 41.0 48-49 36.558412108113274 39.0 36.0 41.0 30.0 41.0 50-51 36.335446973936556 39.0 35.0 41.0 30.0 41.0 52-53 36.13533801249553 39.0 35.0 41.0 29.0 41.0 54-55 35.89800262628546 39.0 35.0 40.0 28.5 41.0 56-57 35.637964527686734 38.0 35.0 40.0 28.0 41.0 58-59 35.33483163929651 38.0 34.0 40.0 28.0 41.0 60-61 34.98445444387165 38.0 34.0 40.0 27.0 41.0 62-63 34.61454080437114 37.0 34.0 40.0 26.0 41.0 64-65 34.24249836201756 37.0 33.5 39.5 26.0 41.0 66-67 33.845924318319035 36.0 33.0 39.0 26.0 40.5 68-69 33.400757216541734 36.0 33.0 39.0 25.5 40.0 70-71 32.91940643926968 35.0 32.0 38.0 24.5 40.0 72-73 32.552526628126984 35.0 32.0 37.5 23.5 39.5 74-75 32.122121469837694 35.0 31.5 37.0 22.5 39.0 76-77 30.07652226691257 33.0 29.0 35.0 20.0 37.5 78-79 31.151122488924667 34.0 31.0 36.0 20.5 38.0 80-81 31.201008703131418 34.0 31.0 36.0 21.0 37.0 82-83 30.89888284083589 34.0 31.0 35.0 20.0 37.0 84-85 30.519704262613843 34.0 31.0 35.0 18.5 36.5 86-87 29.99224324567715 34.0 30.0 35.0 14.5 36.0 88-89 29.489683352492904 34.0 29.5 35.0 7.0 36.0 90-91 29.080964116927596 34.0 29.0 35.0 4.5 35.0 92-93 28.797921554194936 34.0 29.0 35.0 2.0 35.0 94-95 28.413085504062103 34.0 29.0 35.0 2.0 35.0 96-97 27.889360488463367 33.5 28.5 35.0 2.0 35.0 98-99 27.142855042458894 33.0 27.0 35.0 2.0 35.0 100-101 23.898489081801717 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.010577714904904667 1101 2 0.06742035588884931 1101 3 0.08344043007085844 1101 4 0.08598127943697165 1101 5 0.03861027690886232 1101 6 0.13008754056405536 1101 7 0.07530076837108624 1101 8 0.06465523195711853 1101 9 0.18307775052904418 1101 10-11 0.13687158470154515 1101 12-13 0.14442365803158452 1101 14-15 0.22153427150244198 1101 16-17 0.15946700979401385 1101 18-19 0.1716723741257482 1101 20-21 0.17959385935733252 1101 22-23 0.17666015223196752 1101 24-25 0.18139992779607184 1101 26-27 0.2015429897244232 1101 28-29 0.24382207126047462 1101 30-31 0.16578790596643245 1101 32-33 0.26825134779225124 1101 34-35 0.2758996064219019 1101 36-37 0.22369169255404842 1101 38-39 0.2474880411234892 1101 40-41 0.32317391151807584 1101 42-43 0.34801052261338583 1101 44-45 0.4296489001909407 1101 46-47 0.49207029655492107 1101 48-49 0.39366035705190683 1101 50-51 0.32522916882903274 1101 52-53 0.41383305394633396 1101 54-55 0.3370482261868659 1101 56-57 0.2503443255577835 1101 58-59 0.33833743840948927 1101 60-61 0.33505273754465037 1101 62-63 0.255351769698855 1101 64-65 0.22463617800696056 1101 66-67 0.20041518396765667 1101 68-69 0.22123107837417422 1101 70-71 0.14280409447119524 1101 72-73 0.2520322550644778 1101 74-75 0.23945583024531203 1101 76-77 0.2646799288476096 1101 78-79 0.3810158831875725 1101 80-81 0.483646255576236 1101 82-83 0.43388868214698206 1101 84-85 0.4638905494322998 1101 86-87 0.5812411695710011 1101 88-89 0.6279360393856805 1101 90-91 0.7792557440925378 1101 92-93 0.6984660575222819 1101 94-95 0.7508741077695653 1101 96-97 0.5148413043243707 1101 98-99 0.6048504157081354 1101 100-101 1.2342628925421835 1102 1 0.10777330455015743 1102 2 0.08371249629940536 1102 3 0.17704852131857507 1102 4 0.09522458924335808 1102 5 0.08410232956733665 1102 6 0.09579471222048852 1102 7 0.09543770495666593 1102 8 0.12108875975340538 1102 9 0.1546402522217889 1102 10-11 0.1866249625248244 1102 12-13 0.20091598062703042 1102 14-15 0.19017565347137122 1102 16-17 0.24353420604269616 1102 18-19 0.29866361065372615 1102 20-21 0.28435140811300386 1102 22-23 0.22479560100443763 1102 24-25 0.13130754886596208 1102 26-27 0.20991058428813147 1102 28-29 0.19540923742788863 1102 30-31 0.2753860384359754 1102 32-33 0.26076420816713153 1102 34-35 0.29691169400064865 1102 36-37 0.28817841508593034 1102 38-39 0.3459244154457153 1102 40-41 0.409050317402162 1102 42-43 0.3742291661395001 1102 44-45 0.25019916082906946 1102 46-47 0.29675848776057023 1102 48-49 0.24228710915949137 1102 50-51 0.28898121592626325 1102 52-53 0.2201137829747566 1102 54-55 0.23711410795988996 1102 56-57 0.31875167939909943 1102 58-59 0.20006053454135753 1102 60-61 0.16791187558806797 1102 62-63 0.12930869805091305 1102 64-65 0.270904186594386 1102 66-67 0.22310591384471223 1102 68-69 0.26573885418385146 1102 70-71 0.24343675359407513 1102 72-73 0.27529197414947504 1102 74-75 0.34163611292981244 1102 76-77 0.25745335964714755 1102 78-79 0.18906282603857605 1102 80-81 0.19111673855575972 1102 82-83 0.28129812235729545 1102 84-85 0.3919381287567525 1102 86-87 0.3345977770682076 1102 88-89 0.4048315566264371 1102 90-91 0.39046932291503467 1102 92-93 0.36651682824197707 1102 94-95 0.342748598816506 1102 96-97 0.4653508667736972 1102 98-99 0.5892982321076659 1102 100-101 1.131594759729584 1103 1 0.0411657395383358 1103 2 0.0666741904132806 1103 3 0.008099929741064216 1103 4 0.08412549880780773 1103 5 0.04535568533926693 1103 6 0.0685558613970727 1103 7 0.09486471942154395 1103 8 0.08030260757165308 1103 9 0.15701533736824302 1103 10-11 0.08430046438802918 1103 12-13 0.1068717245065045 1103 14-15 0.1373913644943343 1103 16-17 0.11182115960281891 1103 18-19 0.11607205317486091 1103 20-21 0.11668351934432764 1103 22-23 0.06830443620096815 1103 24-25 0.06961393585157083 1103 26-27 0.12235498866365191 1103 28-29 0.23069793398596516 1103 30-31 0.15108474020301088 1103 32-33 0.21207145663449012 1103 34-35 0.1299870836708834 1103 36-37 0.046484701899245806 1103 38-39 0.20399279305519968 1103 40-41 0.19789262617324965 1103 42-43 0.24818737425746207 1103 44-45 0.16582052958332838 1103 46-47 0.1700325190944625 1103 48-49 0.23955140374618367 1103 50-51 0.2569856836106865 1103 52-53 0.07940376173823438 1103 54-55 0.024098220633689493 1103 56-57 0.030966689215986776 1103 58-59 -0.10303128338547651 1103 60-61 0.018921612335283555 1103 62-63 0.05104358205138482 1103 64-65 0.18099955062051265 1103 66-67 0.05599448218224978 1103 68-69 -0.013455359864309457 1103 70-71 -0.04194059544628459 1103 72-73 0.09663167844085763 1103 74-75 0.33615418401206654 1103 76-77 0.15164486904330587 1103 78-79 0.10844165762890867 1103 80-81 0.23611112890716512 1103 82-83 0.1979552726558076 1103 84-85 0.0900759257676107 1103 86-87 0.23868180159038488 1103 88-89 0.34171800131129615 1103 90-91 0.5250758461349712 1103 92-93 0.46989513893636925 1103 94-95 0.27913217857368977 1103 96-97 0.20550743607289235 1103 98-99 0.4070222173623037 1103 100-101 0.9927679776644318 1104 1 -0.022124083509545756 1104 2 0.030733658145543075 1104 3 -0.05750654585965265 1104 4 -0.00979761611403518 1104 5 0.04725443626548298 1104 6 0.031543229520991645 1104 7 -0.048647219994848 1104 8 -0.05069532828100165 1104 9 -0.12230658140667572 1104 10-11 0.008431361321797226 1104 12-13 -0.023297740667629796 1104 14-15 0.030338144336262474 1104 16-17 0.06418158707538169 1104 18-19 -0.0033226620786592775 1104 20-21 -0.03116208199296011 1104 22-23 -0.018813112138893473 1104 24-25 0.03827737839065293 1104 26-27 0.009278416736059114 1104 28-29 0.06027958027579672 1104 30-31 -0.05281955260599602 1104 32-33 -0.062504687467694 1104 34-35 -0.06981399956541878 1104 36-37 -0.1497157563591074 1104 38-39 -0.02675864699632058 1104 40-41 0.027842830823757936 1104 42-43 -0.002503431465690653 1104 44-45 -0.04422890189403006 1104 46-47 -0.09163763192349705 1104 48-49 -0.12983818421825077 1104 50-51 -0.16983610284709982 1104 52-53 -0.16074059625901072 1104 54-55 -0.16494735379911418 1104 56-57 -0.221655395396418 1104 58-59 -0.2283555155050223 1104 60-61 -0.21508003280779775 1104 62-63 -0.33010701469890336 1104 64-65 -0.14853683218179015 1104 66-67 -0.3295053237526133 1104 68-69 -0.22768386274000108 1104 70-71 -0.34004107267008976 1104 72-73 -0.4005304497479898 1104 74-75 -0.44896113037344776 1104 76-77 -0.39863084679240757 1104 78-79 -0.3205881159323596 1104 80-81 -0.22499347661134195 1104 82-83 -0.25291090840696384 1104 84-85 -0.1653006157270447 1104 86-87 -0.03257698647284357 1104 88-89 0.16387246613257744 1104 90-91 0.2271657401930547 1104 92-93 0.22255421721519042 1104 94-95 0.1815092008206065 1104 96-97 0.24106226388419216 1104 98-99 0.2699328415463462 1104 100-101 0.8705625707451254 1105 1 -0.09404233790794336 1105 2 -0.0456655087295772 1105 3 -0.03312991296268564 1105 4 -0.10747612302083809 1105 5 -0.04010728862959212 1105 6 -0.10076311584713693 1105 7 -0.017738130664966434 1105 8 -0.039277209378305145 1105 9 -0.0011925287648608673 1105 10-11 3.7892100939274087E-4 1105 12-13 -0.05094950454378022 1105 14-15 -0.07873959024502852 1105 16-17 -0.05459820460865217 1105 18-19 -0.07649946312927369 1105 20-21 -0.06706996402136411 1105 22-23 -0.06189271239181693 1105 24-25 -0.09078184712978299 1105 26-27 -0.01934287971464954 1105 28-29 0.07268503727849662 1105 30-31 -0.08371887061094441 1105 32-33 0.0010080560771541514 1105 34-35 0.03249741820606289 1105 36-37 0.006514483850459385 1105 38-39 -0.02585816511800232 1105 40-41 -0.016611051996775927 1105 42-43 -0.07069989676489996 1105 44-45 -0.012768492697006195 1105 46-47 -0.029249676449474293 1105 48-49 -0.01744471337414666 1105 50-51 0.06875533243459131 1105 52-53 0.04864630304336259 1105 54-55 0.09178183197592915 1105 56-57 -0.016937018009606675 1105 58-59 -0.025604648679923514 1105 60-61 -0.10890915882576024 1105 62-63 -0.14587042008816553 1105 64-65 -0.06961622919115484 1105 66-67 -0.0348820712997977 1105 68-69 -0.16800321569589016 1105 70-71 -0.1377186241584596 1105 72-73 -0.3093709365597448 1105 74-75 -0.24320959822969712 1105 76-77 -0.2884374440435842 1105 78-79 -0.4376270917932814 1105 80-81 -0.26012551543954743 1105 82-83 -0.2967339979175101 1105 84-85 -0.1868159875411557 1105 86-87 -0.12344097136680787 1105 88-89 0.06659154358053243 1105 90-91 0.13239322532326625 1105 92-93 0.10551263566761904 1105 94-95 0.06677712620763998 1105 96-97 0.11495528133881905 1105 98-99 0.29101458367370014 1105 100-101 0.7271796685660767 1106 1 -0.042772445565958606 1106 2 -0.061225084774783056 1106 3 -0.07379206987681641 1106 4 -0.02191105033632823 1106 5 -0.06176517191747166 1106 6 -0.16971620715535352 1106 7 -0.08115598509873223 1106 8 -0.04149478547714125 1106 9 -0.06612022914568882 1106 10-11 -0.09165221695495518 1106 12-13 0.05817997655790208 1106 14-15 0.10178603507878137 1106 16-17 -0.0952762546380157 1106 18-19 -0.12424740870086026 1106 20-21 -0.09585915298379888 1106 22-23 -0.1281158097805033 1106 24-25 -0.08709686078272938 1106 26-27 -0.1243789105961568 1106 28-29 -0.10793659543529799 1106 30-31 -0.033148815921329344 1106 32-33 -0.035266141461079314 1106 34-35 -0.05316463491629975 1106 36-37 0.03972839177504994 1106 38-39 -0.06354939064800647 1106 40-41 0.034823501668327594 1106 42-43 0.07934467963925584 1106 44-45 -0.0038848899807746307 1106 46-47 0.003007509184712376 1106 48-49 0.028259885725802292 1106 50-51 0.10410718870720359 1106 52-53 0.14733157481779102 1106 54-55 0.18091755788261565 1106 56-57 0.017559150776577326 1106 58-59 -0.023689960760165718 1106 60-61 0.043192765127734845 1106 62-63 0.03782863869344766 1106 64-65 0.04789757295231567 1106 66-67 0.11820214447984512 1106 68-69 0.10960669330466999 1106 70-71 0.0343548370636384 1106 72-73 0.1260366321093329 1106 74-75 0.18885106416939124 1106 76-77 0.01344054915978532 1106 78-79 0.07390822854705448 1106 80-81 0.07683274556389463 1106 82-83 0.20300084653754524 1106 84-85 0.029522286280570853 1106 86-87 0.24443966982416399 1106 88-89 0.3017748355268992 1106 90-91 0.394851265336726 1106 92-93 0.5405526084741759 1106 94-95 0.5723591234381296 1106 96-97 0.5673917823970633 1106 98-99 0.6466806630519173 1106 100-101 1.0603990471820346 1107 1 -0.02454432636683279 1107 2 -0.026090026285032764 1107 3 -0.060068537951565304 1107 4 -0.034731402476069206 1107 5 -0.02103413744497118 1107 6 -0.031456572520710324 1107 7 -0.028073155859772214 1107 8 0.023351101472812275 1107 9 -0.02823479251213712 1107 10-11 -0.0026288120282416116 1107 12-13 -0.06396827854251796 1107 14-15 -0.06910233015783973 1107 16-17 -0.020646583841127608 1107 18-19 0.005688866859095754 1107 20-21 0.054645081239016235 1107 22-23 -0.09307411226346574 1107 24-25 -0.06390552782489323 1107 26-27 0.007767651473642445 1107 28-29 0.0434426456515169 1107 30-31 -0.12758787420118978 1107 32-33 0.05308568049582618 1107 34-35 0.10338052688761223 1107 36-37 0.06739876784983068 1107 38-39 0.04625530117103693 1107 40-41 0.04437096223393411 1107 42-43 0.00784068959945472 1107 44-45 -0.08097189805262417 1107 46-47 -0.12812328762623082 1107 48-49 -0.26323917285783693 1107 50-51 -0.17396517592442962 1107 52-53 -0.09184566859912024 1107 54-55 -0.23211435935559876 1107 56-57 -0.2477255221238508 1107 58-59 -0.19827014705513335 1107 60-61 -0.16714793975716447 1107 62-63 -0.2359544052757485 1107 64-65 -0.16824162900148565 1107 66-67 -0.2495079859504301 1107 68-69 -0.13875792303537082 1107 70-71 -0.1394332148348667 1107 72-73 -0.16640033781622066 1107 74-75 -0.1294799539038607 1107 76-77 -0.2643059283222158 1107 78-79 -0.27536608372830074 1107 80-81 -0.28210001955975983 1107 82-83 -0.10162573134520869 1107 84-85 -0.1033595332791144 1107 86-87 -0.15878876110926754 1107 88-89 0.1584813822340969 1107 90-91 0.21216941244749066 1107 92-93 0.18738374162890636 1107 94-95 0.33038970717575467 1107 96-97 0.39957113233066366 1107 98-99 0.3480316568683186 1107 100-101 0.8547210271908874 1108 1 -0.10099514039876567 1108 2 -0.13392769386285863 1108 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quality scores pass #Quality Count 2 5.0 3 21.0 4 42.0 5 89.0 6 242.0 7 589.0 8 1377.0 9 2603.0 10 4197.0 11 6283.0 12 8684.0 13 12040.0 14 14693.0 15 19105.0 16 24261.0 17 32006.0 18 30892.0 19 23170.0 20 22154.0 21 23516.0 22 25307.0 23 28011.0 24 33498.0 25 40019.0 26 49283.0 27 60215.0 28 74691.0 29 91570.0 30 111018.0 31 134622.0 32 166646.0 33 210537.0 34 276906.0 35 386078.0 36 591034.0 37 949441.0 38 817538.0 39 80527.0 40 6.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.529682008136604 19.477370639950554 14.899653133331489 23.093294218581352 2 15.874737761996785 20.752915976324836 39.203214580754604 24.169131680923776 3 19.827007918370125 24.26741062772633 29.518695054074094 26.386886399829447 4 13.489578020802606 16.94797234773196 36.39158210266405 33.17086752880138 5 14.128391175019228 37.260011449795954 33.854271481462085 14.757325893722737 6 32.435268679662094 35.8661412257898 17.11850171241531 14.580088382132805 7 29.154111864322672 32.22024040895804 20.248311706451492 18.377336020267794 8 26.370598467785733 35.11717202904903 19.260491128245985 19.25173837491925 9 26.234000380434637 17.20042380785662 20.090922958311165 36.47465285339758 10-11 25.294572649690462 26.53275413538878 26.909719829190365 21.262953385730395 12-13 26.299324406903324 23.702651280199298 27.43829653501239 22.559727777884987 14-15 23.00113280723232 25.24651939177142 25.828062329898 25.92428547109825 16-17 22.50043075424494 28.596568209647515 26.266773570297946 22.6362274658096 18-19 22.362691519934316 27.228323315318942 28.43011747203048 21.97886769271626 20-21 23.239915495727463 26.229210487866066 28.401168320270827 22.129705696135648 22-23 22.873184320579583 26.244039627688657 28.272116898189626 22.61065915354213 24-25 22.97165853878182 26.43434883650408 28.269980399346096 22.324012225368005 26-27 22.692401042843443 26.5146173022554 28.36991789279186 22.423063762109294 28-29 22.37200304347706 26.884093329620878 28.410104858444317 22.33379876845774 30-31 22.6077990018645 26.36189166067069 28.294871759528554 22.735437577936263 32-33 22.495345648755915 26.528228433537425 28.318821222371394 22.657604695335266 34-35 23.093277605639027 26.517305142324915 28.03518178299903 22.35423546903702 36-37 22.94754599789162 26.23685823761832 28.22323004965815 22.592365714831914 38-39 22.67126607329144 26.612395261468524 28.372095641404353 22.34424302383568 40-41 22.84594093026702 26.457004974650022 28.227350868194463 22.469703226888495 42-43 22.648723251869647 26.54578161085243 27.870803737630684 22.934691399647235 44-45 22.864705087063363 26.52518111470098 27.7598705487492 22.850243249486454 46-47 22.928046919214992 26.78149352256749 27.50414757617635 22.786311982041166 48-49 23.078580675248656 26.781386725906664 27.413537824653133 22.726494774191547 50-51 22.643664799479335 26.953315875212187 27.512484226007857 22.890535099300628 52-53 23.04248462410026 27.01041095210659 27.102062157873018 22.84504226592013 54-55 22.770517510560737 26.607945110817667 27.380794851083735 23.24074252753786 56-57 22.850992300333843 26.985370266736137 27.245000822437188 22.91863661049283 58-59 23.178186225691785 26.68666252689873 27.204317304712422 22.930833942697067 60-61 23.02705168911957 26.5813692928995 27.69780024255156 22.693778775429376 62-63 22.835313155595006 26.695013181968136 27.572999341131325 22.896674321305536 64-65 22.7107529757064 26.784665727526104 27.31118634037505 23.193394956392453 66-67 22.69154758328849 26.914568385093276 27.035971115592194 23.357912916026045 68-69 22.50157194524002 26.742458510868765 27.380138744699785 23.375830799191434 70-71 22.63868477561426 26.692590743650296 27.247780126947035 23.42094435378841 72-73 22.698806960667287 26.57041854242076 27.379577276473977 23.351197220437978 74-75 22.490096294070458 27.007711612169867 27.053037550000962 23.449154543758713 76-77 22.58691484938684 26.690465765722678 27.219840114942105 23.50277926994838 78-79 22.573309687135243 26.706146962025056 27.23718673364635 23.483356617193348 80-81 22.490278925795458 26.725568205574472 27.218594256629007 23.565558612001062 82-83 22.500985546240727 26.74309589250057 27.26043874956466 23.495479811694047 84-85 22.416111406698406 26.660025141767036 27.151741499261643 23.77212195227291 86-87 22.378977428814785 26.575498730195395 27.37368791842696 23.671835922562856 88-89 22.4006821502669 26.513048405678884 27.09839399893999 23.98787544511423 90-91 22.312548300863597 26.530632697108704 27.308111734433112 23.848707267594587 92-93 22.499584186784215 26.553177226484497 27.184880204442262 23.762358382289023 94-95 22.27535137664661 26.36425548905222 27.168302984127074 24.192090150174096 96-97 22.143882646010475 26.282499625825494 27.35426405589541 24.21935367226862 98-99 22.05464207958402 26.37119223029932 27.0538047085294 24.520360981587256 100-101 22.021784390246705 25.902714003061188 27.623054811317775 24.452446795374335 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 919.0 1 632.0 2 404.5 3 495.0 4 461.5 5 432.5 6 805.0 7 1784.5 8 2883.5 9 3364.5 10 3105.0 11 2865.5 12 2978.5 13 3283.0 14 3624.0 15 3845.5 16 4048.5 17 4339.0 18 4705.0 19 5082.5 20 5700.0 21 6540.0 22 7706.5 23 9351.5 24 11185.5 25 13820.0 26 18470.0 27 24138.0 28 28739.0 29 34023.5 30 40878.0 31 49142.5 32 57902.5 33 66433.5 34 76558.0 35 84989.0 36 94668.0 37 105573.0 38 111724.5 39 114506.5 40 118927.0 41 127459.0 42 136111.0 43 144633.0 44 152956.5 45 158866.5 46 160560.5 47 169026.0 48 193186.0 49 196125.0 50 178963.5 51 170697.0 52 163635.0 53 160434.0 54 157236.0 55 145364.0 56 131445.0 57 117996.0 58 106412.5 59 95130.5 60 80759.5 61 65244.0 62 51100.0 63 40049.5 64 30104.0 65 22140.0 66 16706.5 67 13051.0 68 9677.5 69 6726.5 70 4969.0 71 3855.0 72 3083.5 73 2529.5 74 1994.5 75 1529.0 76 1072.0 77 610.5 78 329.5 79 205.0 80 119.0 81 70.5 82 49.5 83 39.5 84 22.5 85 13.5 86 13.5 87 8.0 88 8.5 89 9.5 90 5.5 91 2.0 92 3.0 93 2.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38953198269849454 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.986503759778502E-4 16-17 0.001975687102622702 18-19 1.7229829383337515E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.182445055222752E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007408826634835131 36-37 0.017459560441782016 38-39 0.010808846299813735 40-41 0.008006127386790832 42-43 0.003641237276345328 44-45 0.0018493350204782264 46-47 0.0016655501737226266 48-49 2.2973105844450018E-5 50-51 5.283814344223505E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.445965876667503E-4 60-61 6.202738578001505E-4 62-63 0.0 64-65 0.0 66-67 0.0011141956334558259 68-69 6.662200694890506E-4 70-71 0.0017689291500226515 72-73 0.0 74-75 0.0 76-77 0.09860057028437948 78-79 0.03553939474136418 80-81 4.5946211688900035E-5 82-83 0.0 84-85 0.0 86-87 0.15111709024479222 88-89 0.3281133842233574 90-91 0.28221311874614624 92-93 0.0 94-95 3.445965876667503E-5 96-97 1.1486552922225009E-5 98-99 1.6081174091115013E-4 100-101 6.547335165668256E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4352916.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.76481172856764 #Duplication Level Percentage of deduplicated Percentage of total 1 69.40513264023406 30.375025629961083 2 15.556144649667273 13.61623483630106 3 5.975290094275321 7.845223379986038 4 2.9097054629362784 5.093708470839639 5 1.6518155336181037 3.6145697919559896 6 0.9736979985741856 2.556822575284941 7 0.6379665052410541 1.9544338793704898 8 0.48217709748208926 1.6881911912904675 9 0.3324353675954759 1.309407414725986 >10 1.8030712265077866 14.559715856497437 >50 0.16061499363658358 4.857620099405056 >100 0.10411012166923121 8.425710939400236 >500 0.005418428188632666 1.6197342897856533 >1k 0.0022094559936244296 1.6054369401882318 >5k 1.052121901725903E-4 0.37700094498339415 >10k+ 1.052121901725903E-4 0.5011637600243374 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11741 0.2697272357196877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10027 0.23035133230230034 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9905 0.22754861338927743 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6470 0.1486359948135916 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4720 0.1084330595858041 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.297310584445002E-5 5 0.0 0.0 0.0 0.0 2.297310584445002E-5 6 0.0 0.0 0.0 0.0 4.594621168890004E-5 7 2.297310584445002E-5 0.0 0.0 0.0 4.594621168890004E-5 8 2.297310584445002E-5 0.0 0.0 0.0 6.891931753335006E-5 9 2.297310584445002E-5 0.0 0.0 0.0 6.891931753335006E-5 10-11 2.297310584445002E-5 0.0 0.0 0.0 6.891931753335006E-5 12-13 2.297310584445002E-5 0.0 0.0 6.891931753335006E-5 6.891931753335006E-5 14-15 2.297310584445002E-5 0.0 0.0 1.148655292222501E-4 1.0337897630002509E-4 16-17 2.297310584445002E-5 0.0 0.0 1.263520821444751E-4 1.6081174091115013E-4 18-19 5.7432764611125054E-5 0.0 0.0 1.3783863506670012E-4 1.6081174091115013E-4 20-21 6.891931753335006E-5 0.0 0.0 2.0675795260005018E-4 1.6081174091115013E-4 22-23 6.891931753335006E-5 0.0 0.0 3.216234818223003E-4 1.8378484675560014E-4 24-25 9.189242337780008E-5 0.0 0.0 6.891931753335006E-4 2.297310584445002E-4 26-27 1.3783863506670012E-4 0.0 0.0 0.001263520821444751 2.527041642889502E-4 28-29 1.6081174091115013E-4 0.0 0.0 0.0031013692890007527 2.527041642889502E-4 30-31 1.6081174091115013E-4 0.0 0.0 0.010050733806946884 2.527041642889502E-4 32-33 1.6081174091115013E-4 0.0 0.0 0.024983252605839397 2.527041642889502E-4 34-35 1.6081174091115013E-4 0.0 0.0 0.04652053933501129 2.7567727013340024E-4 36-37 1.6081174091115013E-4 0.0 0.0 0.08263426172248672 2.7567727013340024E-4 38-39 1.8378484675560017E-4 0.0 0.0 0.13824066441897798 2.7567727013340024E-4 40-41 1.8378484675560017E-4 0.0 0.0 0.19675316500479217 2.7567727013340024E-4 42-43 1.8378484675560017E-4 0.0 0.0 0.25896433563156285 2.7567727013340024E-4 44-45 2.0675795260005018E-4 0.0 0.0 0.33501680252961463 2.7567727013340024E-4 46-47 2.1824450552227517E-4 0.0 0.0 0.4301369472785599 2.871638230556253E-4 48-49 2.297310584445002E-4 0.0 0.0 0.5347564712941852 3.2162348182230026E-4 50-51 2.297310584445002E-4 0.0 0.0 0.6465091446745124 3.2162348182230026E-4 52-53 2.297310584445002E-4 0.0 0.0 0.7595253388762844 3.2162348182230026E-4 54-55 2.297310584445002E-4 0.0 0.0 0.8886341937220934 3.2162348182230026E-4 56-57 2.527041642889502E-4 0.0 0.0 1.0451269907344871 3.2162348182230026E-4 58-59 2.527041642889502E-4 0.0 0.0 1.2184475877779402 3.2162348182230026E-4 60-61 2.527041642889502E-4 0.0 0.0 1.4037946057309627 3.2162348182230026E-4 62-63 2.527041642889502E-4 0.0 0.0 1.5942071935226867 3.445965876667503E-4 64-65 2.527041642889502E-4 0.0 0.0 1.7886745344959563 3.445965876667503E-4 66-67 2.7567727013340024E-4 0.0 0.0 2.010996766305621 3.445965876667503E-4 68-69 2.7567727013340024E-4 0.0 0.0 2.270386104395307 3.445965876667503E-4 70-71 2.871638230556253E-4 0.0 0.0 2.5569181670402092 3.445965876667503E-4 72-73 3.1013692890007527E-4 0.0 0.0 2.84364550108479 4.3648901104455034E-4 74-75 3.2162348182230026E-4 0.0 0.0 3.1364262485193835 4.3648901104455034E-4 76-77 3.2162348182230026E-4 0.0 0.0 3.462414620452129 4.3648901104455034E-4 78-79 3.2162348182230026E-4 0.0 0.0 3.8344755561559194 4.3648901104455034E-4 80-81 3.2162348182230026E-4 0.0 0.0 4.238917084547462 4.824352227334504E-4 82-83 3.2162348182230026E-4 0.0 0.0 4.648217424825106 5.283814344223505E-4 84-85 3.2162348182230026E-4 0.0 0.0 5.047834600989313 5.283814344223505E-4 86-87 3.2162348182230026E-4 0.0 0.0 5.47387084887464 5.283814344223505E-4 88-89 3.2162348182230026E-4 0.0 0.0 5.942303963595897 5.283814344223505E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18175 0.0 21.964893 1 GTATCAA 28455 0.0 21.094606 1 TACATGG 18955 0.0 20.803782 2 GAGTACT 12805 0.0 20.624128 12-13 ACATGGG 18580 0.0 20.176529 3 GGTATCA 22390 0.0 18.936312 1 GTACTTT 13970 0.0 18.717386 14-15 AGTACTT 13805 0.0 18.511402 12-13 AGAGTAC 24580 0.0 18.157747 10-11 ATCAACG 32895 0.0 18.133034 3 TCAACGC 32965 0.0 18.123318 4 CAACGCA 33060 0.0 18.085594 5 TATCAAC 33025 0.0 18.076023 2 AACGCAG 33580 0.0 17.833794 6 GTACACG 565 0.0 17.706402 1 TACCTGG 3300 0.0 17.687084 2 ACTTTTT 16025 0.0 16.509232 16-17 ACGCAGA 36925 0.0 16.076885 7 AGTACAT 14225 0.0 16.045671 2 CGCAGAG 37070 0.0 15.937194 8 >>END_MODULE