FastQCFastQC Report
Thu 26 May 2016
SRR938726_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938726_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4368418
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121890.2790254961864913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95620.21888930958530067No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90420.2069856868092751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60150.13769286730344943No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52000.11903622776025555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG175950.024.1467571
GTATCAA263300.023.2294141
TACATGG180350.022.7276732
ACATGGG175400.022.1519363
GAGTACT118850.021.28603412-13
GGTATCA201750.020.7055071
ATCAACG305050.019.8288043
TCAACGC306750.019.7034474
TATCAAC307800.019.682472
CAACGCA306000.019.6742215
GTACTTT129050.019.4018714-15
AACGCAG310050.019.3702136
AGAGTAC225750.018.99490710-11
AGTACTT128200.018.54882612-13
TACCTGG37500.018.0909822
CATGGGG96650.017.9163024
CATGGGA82550.017.7581924
ACGCAGA339600.017.5454447
CGCAGAG340750.017.3470088
GTGTAGC22850.016.8464871
Produced by FastQC (version 0.11.5)