FastQCFastQC Report
Thu 26 May 2016
SRR938726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4368418
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119530.2736230827727566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102690.23507365824424312No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98680.2258941337573465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64590.14785672982759432No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47320.10832296726183255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG180250.024.5938361
TACATGG185350.023.1977352
ACATGGG180750.022.8691393
GTATCAA277950.021.168431
GAGTACT128700.020.9023512-13
GTACTTT135200.019.79215214-15
CATGGGG100450.019.417884
AGTACTT138150.019.07760212-13
TACCTGG35550.018.9565242
GGTATCA217700.018.7899511
AGAGTAC240700.018.44974510-11
TCAACGC321000.018.1996464
ATCAACG320500.018.1984253
CAACGCA322900.018.0778585
TATCAAC326000.017.9350682
AACGCAG327250.017.847366
CATGGGA84700.016.8092274
GTACCTG41400.016.7740571
ACTTTTT160700.016.75456416-17
ATGGGGA61350.016.1674565
Produced by FastQC (version 0.11.5)