##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938726_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4368418 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08168471973149 33.0 31.0 34.0 30.0 34.0 2 32.28990334716138 34.0 31.0 34.0 30.0 34.0 3 32.37320718850623 34.0 31.0 34.0 30.0 34.0 4 35.97896034674338 37.0 35.0 37.0 35.0 37.0 5 35.881421603884974 37.0 35.0 37.0 35.0 37.0 6 35.905966416217495 37.0 35.0 37.0 35.0 37.0 7 35.8307515443806 37.0 35.0 37.0 35.0 37.0 8 35.81257150758009 37.0 35.0 37.0 33.0 37.0 9 37.46744153146517 39.0 37.0 39.0 34.0 39.0 10-11 37.38921275390771 39.0 37.0 39.0 34.0 39.0 12-13 37.31024789752263 39.0 37.0 39.0 34.0 39.0 14-15 38.53865598484394 40.0 38.0 41.0 34.0 41.0 16-17 38.46355156489146 40.0 38.0 41.0 33.5 41.0 18-19 38.604223199336694 40.0 38.0 41.0 34.0 41.0 20-21 38.60603346566194 40.0 38.0 41.0 34.0 41.0 22-23 38.5350147353115 40.0 38.0 41.0 34.0 41.0 24-25 38.45938254992997 40.0 38.0 41.0 34.0 41.0 26-27 38.29195111365259 40.0 38.0 41.0 33.5 41.0 28-29 38.127963715926455 40.0 38.0 41.0 33.0 41.0 30-31 37.927830853183 40.0 37.0 41.0 33.0 41.0 32-33 37.80859901685233 40.0 37.0 41.0 33.0 41.0 34-35 37.79927207057566 40.0 37.0 41.0 33.0 41.0 36-37 37.70580150525889 40.0 37.0 41.0 33.0 41.0 38-39 37.5922459801237 40.0 37.0 41.0 32.0 41.0 40-41 37.4444095093464 40.0 37.0 41.0 32.0 41.0 42-43 37.26165021296039 40.0 36.5 41.0 31.0 41.0 44-45 37.04124353484488 40.0 36.0 41.0 31.0 41.0 46-47 36.76501596230031 40.0 36.0 41.0 30.5 41.0 48-49 36.491789476190235 39.0 36.0 41.0 30.0 41.0 50-51 36.2700221224251 39.0 35.0 41.0 30.0 41.0 52-53 36.05809219722106 39.0 35.0 40.5 29.0 41.0 54-55 35.78478444599395 39.0 35.0 40.0 28.0 41.0 56-57 35.50901081352563 38.0 35.0 40.0 28.0 41.0 58-59 35.20043594729259 38.0 34.0 40.0 27.5 41.0 60-61 34.82703418033714 38.0 34.0 40.0 26.5 41.0 62-63 34.46013808660252 37.0 34.0 40.0 26.0 41.0 64-65 34.04699103886121 36.5 33.0 39.0 26.0 41.0 66-67 33.635692944219166 36.0 33.0 39.0 25.5 40.0 68-69 33.278700779092105 35.5 32.5 39.0 25.0 40.0 70-71 32.834068305734476 35.0 32.0 38.0 24.0 40.0 72-73 32.33898450194098 35.0 31.5 37.5 22.5 39.5 74-75 31.834387299933294 35.0 31.0 37.0 21.5 39.0 76-77 29.90116330442737 33.0 28.5 35.0 19.5 37.0 78-79 30.861867270943392 34.0 30.5 36.0 20.0 38.0 80-81 30.913528993791346 34.0 31.0 35.5 20.0 37.0 82-83 30.591325051769314 34.0 31.0 35.0 18.5 37.0 84-85 30.192228742762254 34.0 30.0 35.0 16.5 36.0 86-87 29.612791404119296 34.0 29.5 35.0 8.5 36.0 88-89 29.01868628414222 34.0 29.0 35.0 3.5 35.5 90-91 28.495118484540626 33.5 29.0 35.0 2.0 35.0 92-93 28.187016672855023 33.0 28.5 35.0 2.0 35.0 94-95 27.830744219074276 33.0 27.0 35.0 2.0 35.0 96-97 27.20083311624483 33.0 26.5 35.0 2.0 35.0 98-99 26.3355786923321 33.0 25.0 35.0 2.0 35.0 100-101 22.545058417028773 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12375129335164559 1101 2 0.11385368305867871 1101 3 0.017456421030082936 1101 4 0.060674991949746016 1101 5 -0.05403346482096083 1101 6 0.058061685504355864 1101 7 0.14771760345018947 1101 8 0.13067187498027977 1101 9 0.15062759619996058 1101 10-11 0.21635084187238363 1101 12-13 0.17930365982503815 1101 14-15 0.2702773322900356 1101 16-17 0.2649576859218499 1101 18-19 0.24992742886219332 1101 20-21 0.19531855425836397 1101 22-23 0.11889856697204237 1101 24-25 0.12760536573796344 1101 26-27 0.05322163176205663 1101 28-29 0.07017020544979147 1101 30-31 0.14560534545308457 1101 32-33 0.2158707191859861 1101 34-35 0.27356056069321966 1101 36-37 0.33302016583211724 1101 38-39 0.33808321885499026 1101 40-41 0.37842212330836134 1101 42-43 0.32976279943884634 1101 44-45 0.2617418943366232 1101 46-47 0.2450094395727973 1101 48-49 0.20436667062227798 1101 50-51 0.23880917610804886 1101 52-53 0.14655635509862464 1101 54-55 0.32384258817734946 1101 56-57 0.3140258084671288 1101 58-59 0.2420341719852388 1101 60-61 0.30649188429417507 1101 62-63 0.22423119575575612 1101 64-65 0.14234705624080135 1101 66-67 0.14652183964964394 1101 68-69 0.11767471585758216 1101 70-71 0.26179248371741437 1101 72-73 0.1310827565039432 1101 74-75 -0.00727455981413172 1101 76-77 -0.007257600585361956 1101 78-79 0.023547835225468106 1101 80-81 0.15146171507350914 1101 82-83 0.2780317850259202 1101 84-85 0.2977021547499561 1101 86-87 0.3728441024653719 1101 88-89 0.5245493591197388 1101 90-91 0.43163191715416716 1101 92-93 0.4241428744079023 1101 94-95 0.44345707921151956 1101 96-97 0.3839488636999846 1101 98-99 0.1992116834624582 1101 100-101 0.6849931565478897 1102 1 0.24508144693182032 1102 2 0.1696533771671156 1102 3 0.18692964710285054 1102 4 0.131259858517069 1102 5 0.01904065475898875 1102 6 -0.005180575175849356 1102 7 0.12362411145718255 1102 8 0.1199060067475628 1102 9 0.17329774082953264 1102 10-11 0.18293149811508158 1102 12-13 0.22044471023111356 1102 14-15 0.2681282584880833 1102 16-17 0.31523963655022413 1102 18-19 0.26410693020025633 1102 20-21 0.23075062501218468 1102 22-23 0.24059913611677786 1102 24-25 0.28845632192961546 1102 26-27 0.22674639439184574 1102 28-29 0.28055100656805365 1102 30-31 0.25823670771060137 1102 32-33 0.34313572148837324 1102 34-35 0.33216228280057436 1102 36-37 0.2884202221941834 1102 38-39 0.34262697951728427 1102 40-41 0.400204651583266 1102 42-43 0.3783288841865158 1102 44-45 0.39072723998157244 1102 46-47 0.3985606764095948 1102 48-49 0.34169903013462744 1102 50-51 0.33854024248626047 1102 52-53 0.21995937931662013 1102 54-55 0.24679698690061969 1102 56-57 0.2625493735107369 1102 58-59 0.2596472392257141 1102 60-61 0.26962322259996796 1102 62-63 0.19891975321895927 1102 64-65 0.24330656552824337 1102 66-67 0.2475515048304402 1102 68-69 0.23365459921485154 1102 70-71 0.25019871660325776 1102 72-73 0.2390797299555132 1102 74-75 0.20561731520016124 1102 76-77 0.12008101011108963 1102 78-79 -0.04097182374514219 1102 80-81 0.18589887222400847 1102 82-83 0.22807002686582578 1102 84-85 0.44356502314796487 1102 86-87 0.4087752214079039 1102 88-89 0.32051695157447213 1102 90-91 0.2848807850576094 1102 92-93 0.4580691153984944 1102 94-95 0.6185812397683392 1102 96-97 0.638841111152793 1102 98-99 0.7582822443773019 1102 100-101 1.2143726159380677 1103 1 0.13246189062333258 1103 2 0.14896743517142852 1103 3 0.11757884343068525 1103 4 0.012286971092898114 1103 5 -0.051335958329531195 1103 6 0.021709520469194388 1103 7 0.02161844614283126 1103 8 0.02630229068543599 1103 9 0.10617388238008374 1103 10-11 0.13788997705363215 1103 12-13 0.1964657185336094 1103 14-15 0.19726017423539588 1103 16-17 0.02498465453725629 1103 18-19 0.011748811688185867 1103 20-21 0.11481787147051392 1103 22-23 0.1675426870205925 1103 24-25 0.18002059341203136 1103 26-27 0.12123891665701336 1103 28-29 0.1003617740947007 1103 30-31 0.19170634409481835 1103 32-33 0.20463323176790027 1103 34-35 0.1343690376248361 1103 36-37 0.22978126081228822 1103 38-39 0.16648482882403925 1103 40-41 0.1714667272419561 1103 42-43 0.17506515557290214 1103 44-45 0.2771933286855841 1103 46-47 0.2635252767683056 1103 48-49 0.13994958140538216 1103 50-51 0.19524971336921482 1103 52-53 0.20462903696291335 1103 54-55 0.19760914675027408 1103 56-57 0.16246308206254412 1103 58-59 0.2533554223813468 1103 60-61 0.24736255812581476 1103 62-63 0.11877930349177035 1103 64-65 0.10077135450214314 1103 66-67 0.2155812305839575 1103 68-69 0.10877119769426713 1103 70-71 0.11372662650693144 1103 72-73 0.21528056569226095 1103 74-75 0.3201142356285622 1103 76-77 0.24156826658316177 1103 78-79 0.06765144828254321 1103 80-81 0.27340110131258655 1103 82-83 0.3037921658306395 1103 84-85 0.2523953860778789 1103 86-87 0.33480227396981377 1103 88-89 0.33779860377492454 1103 90-91 0.2865491721234079 1103 92-93 0.39137607118366446 1103 94-95 0.5170196774858908 1103 96-97 0.6564457668614878 1103 98-99 0.6553675824614515 1103 100-101 1.381297056694038 1104 1 0.1315879029705158 1104 2 0.10777409547115013 1104 3 0.07657571532034524 1104 4 0.07512909505381771 1104 5 -0.06891668920341232 1104 6 0.03480030361665598 1104 7 0.05942724637536401 1104 8 0.039407596603133754 1104 9 0.1235098233108971 1104 10-11 0.07820078845391265 1104 12-13 0.013923802777846106 1104 14-15 0.04950417292341314 1104 16-17 0.12768661856105012 1104 18-19 0.1114298822166262 1104 20-21 0.14153303160686193 1104 22-23 0.08077684189183287 1104 24-25 0.04711535674225331 1104 26-27 0.002267517811198161 1104 28-29 -0.0030263252867754886 1104 30-31 -0.08722398414705168 1104 32-33 -0.015486692759829168 1104 34-35 0.05813558257286644 1104 36-37 0.1484795686631415 1104 38-39 0.061120190899245586 1104 40-41 0.1419807326450595 1104 42-43 0.3209923364106473 1104 44-45 0.30874682574768286 1104 46-47 0.2770685202303369 1104 48-49 0.40757713117391603 1104 50-51 0.3301823182781476 1104 52-53 0.3333482555057543 1104 54-55 0.2905331969610998 1104 56-57 0.22732161664896466 1104 58-59 0.4073888518061324 1104 60-61 0.34712499811059416 1104 62-63 0.34325621637653825 1104 64-65 0.42652676399812606 1104 66-67 0.3822676104694267 1104 68-69 0.3111582954764529 1104 70-71 0.24400311069209835 1104 72-73 0.41790369781234915 1104 74-75 0.3551103632393584 1104 76-77 0.32880009865002435 1104 78-79 0.3214210246622464 1104 80-81 0.4848255302688713 1104 82-83 0.4577460345917679 1104 84-85 0.5783178598603449 1104 86-87 0.5719500417413848 1104 88-89 0.5134206292284667 1104 90-91 0.5135223499823596 1104 92-93 0.5814592892785555 1104 94-95 0.5016358695043834 1104 96-97 0.6214870471805227 1104 98-99 0.8466942537754036 1104 100-101 1.5607484535433613 1105 1 0.06315292498034353 1105 2 0.07647718985138852 1105 3 0.08591429015769592 1105 4 0.024537349668122488 1105 5 -0.008432382294181195 1105 6 -0.05504003124246992 1105 7 -0.006148927376329993 1105 8 0.0715524968341299 1105 9 0.08156963837758013 1105 10-11 0.08759410365759379 1105 12-13 0.08872185462846716 1105 14-15 0.11289387161694009 1105 16-17 0.11988310335853924 1105 18-19 0.11647242702688487 1105 20-21 0.03786781720565813 1105 22-23 -0.002096755917982307 1105 24-25 -0.030339172673649273 1105 26-27 0.050062263414588415 1105 28-29 0.05137266068465607 1105 30-31 0.08765624408331263 1105 32-33 0.10757651231039489 1105 34-35 0.014727434198412936 1105 36-37 0.009563482606424145 1105 38-39 0.16352346515299132 1105 40-41 0.16406543214551306 1105 42-43 0.17418087666434445 1105 44-45 0.1177087200216036 1105 46-47 0.05511456462613751 1105 48-49 -0.028063617473762292 1105 50-51 -0.022278747319802505 1105 52-53 0.08734942941062229 1105 54-55 0.1580115554003072 1105 56-57 0.21114042006102096 1105 58-59 0.1924393590356388 1105 60-61 0.18359802820052806 1105 62-63 0.22077188688247418 1105 64-65 0.20354403384944675 1105 66-67 0.24927989089587044 1105 68-69 0.2505632671879354 1105 70-71 0.20792224646071844 1105 72-73 0.26867733211742717 1105 74-75 0.33101080302072106 1105 76-77 0.32284220984163525 1105 78-79 0.1591374864779027 1105 80-81 0.2161576094857267 1105 82-83 0.3487545217792096 1105 84-85 0.28162307406507026 1105 86-87 0.2837099333787556 1105 88-89 0.3052839320515126 1105 90-91 0.3353203031382783 1105 92-93 0.47883140735144636 1105 94-95 0.5286825477440047 1105 96-97 0.5959183335127989 1105 98-99 0.7826688629552656 1105 100-101 1.4512460475244922 1106 1 0.07627373951152805 1106 2 0.029433755919974658 1106 3 0.0038650523303758177 1106 4 0.024789323804995433 1106 5 -0.14222839131636533 1106 6 -0.031071568156605167 1106 7 -0.007092873339409778 1106 8 0.014995247834164616 1106 9 0.0709454487721004 1106 10-11 -0.0021676090048217134 1106 12-13 -0.013549583749743022 1106 14-15 0.04674587543709663 1106 16-17 0.12162245618746681 1106 18-19 -0.01940960785027812 1106 20-21 0.005095179496315438 1106 22-23 0.06457987552252575 1106 24-25 0.014033477737456224 1106 26-27 -0.004471241611909704 1106 28-29 0.059692960548602514 1106 30-31 0.05171039604432082 1106 32-33 -0.03473172194820506 1106 34-35 -0.0070593716328346545 1106 36-37 0.014338205998058129 1106 38-39 0.2134530925164171 1106 40-41 0.25949693098377935 1106 42-43 0.14938022162974818 1106 44-45 0.17996917833477966 1106 46-47 0.24272572661905656 1106 48-49 0.15535598370919246 1106 50-51 0.1316465302259786 1106 52-53 0.15964922150497074 1106 54-55 0.11858101400487442 1106 56-57 -0.004800875363834223 1106 58-59 0.10568884528373701 1106 60-61 0.10773933455047313 1106 62-63 0.1523334242034764 1106 64-65 0.2118887016835842 1106 66-67 0.16012673945474631 1106 68-69 0.15651019055869142 1106 70-71 0.112140197316144 1106 72-73 0.17044719766954586 1106 74-75 0.30742933176759024 1106 76-77 0.12286799620928335 1106 78-79 0.12012419442500644 1106 80-81 0.12458464253148804 1106 82-83 0.27327116358138426 1106 84-85 0.32854668040490864 1106 86-87 0.3842273686982338 1106 88-89 0.5526937002554284 1106 90-91 0.4195421430530324 1106 92-93 0.5086771892656259 1106 94-95 0.5558993373294498 1106 96-97 0.6610995126840251 1106 98-99 0.9365049139757602 1106 100-101 1.5416558549429311 1107 1 -0.02026292048021361 1107 2 -0.03663954670099656 1107 3 -0.029480290077763982 1107 4 -0.05814303182567215 1107 5 -0.07507002779153993 1107 6 -0.023295668102974787 1107 7 0.012231994801908286 1107 8 -0.005600942443599877 1107 9 -0.011776145184200004 1107 10-11 -0.014396440237291586 1107 12-13 0.03982921044268295 1107 14-15 0.007850941819789625 1107 16-17 0.05049945818222312 1107 18-19 0.06801218847581225 1107 20-21 -0.02357675187158037 1107 22-23 -0.018867712823755767 1107 24-25 -0.010732803481310782 1107 26-27 -0.01203949077562072 1107 28-29 -0.04561444765190714 1107 30-31 -0.04217401878094762 1107 32-33 -0.01148073035552244 1107 34-35 0.028979813640667373 1107 36-37 0.06791888768908905 1107 38-39 -0.04198267774275166 1107 40-41 -0.13831616344130993 1107 42-43 -0.08257988123352789 1107 44-45 -0.020415063829901214 1107 46-47 -0.14616843533079305 1107 48-49 -0.04830316691644043 1107 50-51 -0.17593384858557926 1107 52-53 -0.19575787648521725 1107 54-55 -0.017285703591852553 1107 56-57 -0.14522228778536572 1107 58-59 -0.05237693647328001 1107 60-61 -0.04443037730889188 1107 62-63 -0.12347979272382048 1107 64-65 -0.05281889422503383 1107 66-67 -0.06992522270268609 1107 68-69 -0.05794147701468688 1107 70-71 -0.11012884743670526 1107 72-73 -0.07995385237694563 1107 74-75 0.028240431432301705 1107 76-77 0.03797599106998106 1107 78-79 0.0648688737778862 1107 80-81 0.2068965775262228 1107 82-83 0.2251630028121845 1107 84-85 0.1689106518300605 1107 86-87 0.25413679905331676 1107 88-89 0.1799626437269275 1107 90-91 0.4416619424966619 1107 92-93 0.6488506552871769 1107 94-95 0.6434374142955654 1107 96-97 0.6862074978988808 1107 98-99 0.872826722455617 1107 100-101 1.5158987527085657 1108 1 0.14753696403261785 1108 2 0.05704411186458458 1108 3 0.008793304120700896 1108 4 0.05951249372586176 1108 5 0.040613087305011675 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-1.98751719092445 2316 100-101 -2.0051860467371583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 22.0 4 54.0 5 91.0 6 236.0 7 583.0 8 1380.0 9 2627.0 10 4350.0 11 6492.0 12 8950.0 13 12210.0 14 15262.0 15 19922.0 16 25059.0 17 33257.0 18 32234.0 19 24479.0 20 23949.0 21 25374.0 22 27372.0 23 30879.0 24 36211.0 25 43140.0 26 52469.0 27 63758.0 28 78217.0 29 94828.0 30 114669.0 31 140415.0 32 172081.0 33 218077.0 34 288376.0 35 400713.0 36 609748.0 37 946534.0 38 750902.0 39 63490.0 40 5.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.492194869114485 19.55166070837342 14.892375579663042 23.06376884284905 2 15.89834123016616 20.706947000035253 39.176859906721376 24.217851863077204 3 19.867672919578666 24.179325330130954 29.536001362506976 26.4170003877834 4 13.533228734063451 16.932537133580166 36.376257949674226 33.157976182682155 5 14.139192277104975 37.23799062391303 33.85762114480955 14.765195954172446 6 32.4753987952243 35.871676782771814 17.086969202079903 14.565955219923987 7 29.157054109748653 32.26227435195075 20.246528606007942 18.33414293229265 8 26.388591934196775 35.101128142957016 19.249897789085203 19.26038213376101 9 26.24066652962239 17.14785535633266 20.12353213451643 36.48794597952851 10-11 25.306861660216583 26.531423961717948 26.876388660608942 21.285325717456523 12-13 26.28214607668039 23.670044853766285 27.4572511147056 22.59055795484773 14-15 22.99054257170445 25.199328452542773 25.852940355066757 25.957188620686022 16-17 22.543991269793395 28.552612737984035 26.29173509079028 22.61166090143229 18-19 22.377872264055316 27.216134078744297 28.42016263095702 21.985831026243368 20-21 23.23787467224977 26.26087979675938 28.345421614872933 22.155823916117917 22-23 22.877904541186307 26.24146773500155 28.236091417991595 22.64453630582055 24-25 22.994985008821875 26.427578502667735 28.273424896172 22.304011592338387 26-27 22.722178759407853 26.524775044167058 28.35025521606233 22.40279098036276 28-29 22.38204997781806 26.858247081666637 28.378614409152235 22.38108853136307 30-31 22.648954381197036 26.361614204501493 28.27537337315248 22.714058041148995 32-33 22.483288000369928 26.536895049878467 28.326822204285396 22.652994745466206 34-35 23.104833558679935 26.483430042357227 28.028878094433125 22.38285830452972 36-37 22.924087070652607 26.26976699459257 28.19020274853309 22.61594318622173 38-39 22.67335671898197 26.629349450756262 28.35253664992398 22.344757180337783 40-41 22.883378439742906 26.458203053591177 28.189176545033323 22.469241961632594 42-43 22.64358129764142 26.559827181386286 27.853334029296384 22.943257491675915 44-45 22.82993837334945 26.528498892002272 27.738540006959322 22.903022727688953 46-47 22.946272294786326 26.789317273746576 27.493889064137473 22.770521367329625 48-49 23.12544882620145 26.79051465557046 27.380006450964544 22.704030067263552 50-51 22.68244370499513 26.930138979437857 27.48382937888699 22.903587936680022 52-53 23.068911903576993 27.023650209297738 27.082802057861677 22.824635829263592 54-55 22.838668369189943 26.607366785870767 27.30633835864608 23.247626486293207 56-57 22.881572144549263 27.002154016223283 27.192202825253414 22.924071013974036 58-59 23.186343555156018 26.69181546832146 27.194322221939892 22.92751875458263 60-61 23.056907371642588 26.59860510969884 27.645465324558145 22.69902219410043 62-63 22.877919447447084 26.66685437936003 27.530767712867338 22.924458460325546 64-65 22.71487621231512 26.784768561742982 27.275720916369618 23.224634309572284 66-67 22.722406415061727 26.934857545773017 27.011182632494364 23.331553406670892 68-69 22.52944435160619 26.750671296879187 27.36643309579458 23.353451255720046 70-71 22.650736256490195 26.68009105816719 27.22263318985266 23.446539495489958 72-73 22.73105504097822 26.55867639040037 27.332251629766198 23.378016938855207 74-75 22.49141589858336 27.011393542548973 27.007273036518786 23.48991752234888 76-77 22.60650563617413 26.68730482799439 27.181457126264114 23.52473240956737 78-79 22.58615513891065 26.710015841869687 27.2174577151831 23.486371304036556 80-81 22.545242567049332 26.70082052353859 27.162228378345745 23.591708531066338 82-83 22.54629267705871 26.7162419339276 27.265256218302714 23.472209170710983 84-85 22.413915060326186 26.641120423915478 27.134651491684174 23.810313024074162 86-87 22.42659494212752 26.530994570702326 27.344438201062758 23.697972286107397 88-89 22.435719241496237 26.504280023341405 27.0660014978933 23.993999237269055 90-91 22.348986848886586 26.462908390363243 27.31824499371144 23.869859767038733 92-93 22.52688514187581 26.51378148236493 27.18405007968596 23.775283296073297 94-95 22.309082095800466 26.382813854721494 27.144053232274267 24.164050817203773 96-97 22.204710133730547 26.24632847046672 27.33472193893844 24.21423945686429 98-99 22.044490705788686 26.328066590697137 27.011483333325703 24.615959370188474 100-101 22.13751868973658 25.69369354520624 27.673961487038596 24.494826278018582 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 843.0 1 568.0 2 369.0 3 473.5 4 453.5 5 457.0 6 771.5 7 1697.5 8 2778.0 9 3330.0 10 3182.5 11 2860.0 12 2979.5 13 3245.5 14 3501.0 15 3769.5 16 4052.5 17 4358.0 18 4678.0 19 5014.0 20 5593.5 21 6586.0 22 7808.0 23 9408.5 24 11190.5 25 13935.0 26 18588.5 27 24291.0 28 29071.5 29 34237.0 30 41046.5 31 49378.5 32 57999.5 33 66158.5 34 76284.0 35 85410.0 36 94847.0 37 105278.0 38 111785.0 39 114855.0 40 119109.5 41 127229.5 42 136131.0 43 145094.0 44 153373.0 45 159393.0 46 161149.5 47 169222.0 48 193370.5 49 196841.5 50 179805.0 51 171455.0 52 163914.5 53 160530.0 54 157995.5 55 146445.5 56 132368.5 57 118596.5 58 107069.0 59 95908.5 60 81696.5 61 65886.0 62 51537.0 63 40638.5 64 30508.5 65 22528.5 66 17010.0 67 13196.0 68 9894.5 69 7066.0 70 5261.0 71 3877.5 72 2939.0 73 2412.0 74 1980.5 75 1554.0 76 1058.0 77 604.5 78 351.0 79 223.5 80 145.0 81 102.0 82 73.5 83 47.5 84 32.5 85 20.0 86 12.5 87 8.5 88 8.5 89 10.0 90 10.0 91 6.5 92 4.0 93 2.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23044955862740243 2 0.0 3 0.0 4 0.0 5 0.030926527635404856 6 0.003113255187575914 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.003124700978706708 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.548195250546079E-4 26-27 0.00138494072682605 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007600005310847084 36-37 0.01866808533432469 38-39 0.014639166856285274 40-41 0.009465669265166473 42-43 0.008469885436787413 44-45 0.004074701642562593 46-47 4.578316452317521E-4 48-49 0.0018084349986654208 50-51 4.5783164523175206E-5 52-53 0.0 54-55 0.0 56-57 0.0015222902203955756 58-59 4.349400629701645E-4 60-61 0.0026897609157365437 62-63 9.156632904635042E-4 64-65 0.001636748131703514 66-67 0.005963257179143571 68-69 1.8313265809270082E-4 70-71 3.4337373392381406E-5 72-73 0.0 74-75 5.150606008857211E-4 76-77 0.058545221634010304 78-79 0.1429235938502222 80-81 0.0011102417396869988 82-83 9.957838283790607E-4 84-85 0.0 86-87 0.10726995447779952 88-89 0.357978563406707 90-91 0.39523461353744077 92-93 4.578316452317521E-4 94-95 3.548195250546079E-4 96-97 1.6024107583111324E-4 98-99 0.0 100-101 0.0010186754106406484 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4368418.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.55221140165681 #Duplication Level Percentage of deduplicated Percentage of total 1 69.17973516767923 30.12930450733398 2 15.689368440169202 13.666133821294633 3 5.943473812178598 7.765542837846404 4 2.9108178727195173 5.070902213776056 5 1.6748321573031792 3.6471322088580496 6 0.9953855697530939 2.601074565602721 7 0.6763915706759392 2.062084407346404 8 0.48390663491319513 1.686016324992307 9 0.34719132313885054 1.3608854911947743 >10 1.82595349915275 14.650393594222402 >50 0.1606494195286052 4.837259427862588 >100 0.1041359346309484 8.363882269545098 >500 0.005684700839412675 1.650633610378779 >1k 0.0022633528649403383 1.6250500705734443 >5k 1.0527222627629182E-4 0.3742832708251347 >10k+ 1.0527222627629182E-4 0.5094213783472863 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11953 0.2736230827727566 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10269 0.23507365824424312 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9868 0.2258941337573465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6459 0.14785672982759432 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4732 0.10832296726183255 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.2891582261587603E-5 0.0 3 0.0 0.0 0.0 2.2891582261587603E-5 0.0 4 0.0 0.0 0.0 2.2891582261587603E-5 0.0 5 0.0 0.0 0.0 2.2891582261587603E-5 0.0 6 0.0 0.0 0.0 2.2891582261587603E-5 2.2891582261587603E-5 7 0.0 0.0 0.0 2.2891582261587603E-5 2.2891582261587603E-5 8 0.0 0.0 0.0 2.2891582261587603E-5 2.2891582261587603E-5 9 0.0 0.0 0.0 2.2891582261587603E-5 2.2891582261587603E-5 10-11 0.0 0.0 0.0 2.2891582261587603E-5 3.4337373392381406E-5 12-13 0.0 0.0 0.0 2.2891582261587603E-5 4.5783164523175206E-5 14-15 0.0 0.0 0.0 4.5783164523175206E-5 6.867474678476281E-5 16-17 0.0 0.0 0.0 4.5783164523175206E-5 9.156632904635041E-5 18-19 2.2891582261587603E-5 0.0 0.0 6.867474678476281E-5 1.0301212017714422E-4 20-21 4.5783164523175206E-5 0.0 0.0 1.0301212017714422E-4 1.2590370243873182E-4 22-23 6.867474678476281E-5 0.0 0.0 2.8614477826984506E-4 1.4879528470031943E-4 24-25 6.867474678476281E-5 0.0 0.0 5.837353476704839E-4 1.6024107583111324E-4 26-27 6.867474678476281E-5 0.0 0.0 0.001098795948556205 1.8313265809270082E-4 28-29 6.867474678476281E-5 0.0 0.0 0.0029987972762679765 1.8313265809270082E-4 30-31 6.867474678476281E-5 0.0 0.0 0.009396994518381711 1.8313265809270082E-4 32-33 6.867474678476281E-5 0.0 0.0 0.022136160046955212 2.2891582261587605E-4 34-35 6.867474678476281E-5 0.0 0.0 0.04198316186775167 2.5180740487746364E-4 36-37 6.867474678476281E-5 0.0 0.0 0.07514161877366132 2.6325319600825744E-4 38-39 1.1445791130793803E-4 0.0 0.0 0.12569767819837752 2.7469898713905125E-4 40-41 1.1445791130793803E-4 0.0 0.0 0.1837507308137637 2.7469898713905125E-4 42-43 1.1445791130793803E-4 0.0 0.0 0.2444820985537556 2.8614477826984506E-4 44-45 1.1445791130793803E-4 0.0 0.0 0.31743757122143534 2.9759056940063886E-4 46-47 1.1445791130793803E-4 0.0 0.0 0.40914124976135524 3.548195250546079E-4 48-49 1.3734949356952563E-4 0.0 0.0 0.5071515592143426 3.6626531618540165E-4 50-51 1.3734949356952563E-4 0.0 0.0 0.6153486227737364 3.6626531618540165E-4 52-53 1.3734949356952563E-4 0.0 0.0 0.7228932762386749 3.7771110731619546E-4 54-55 1.3734949356952563E-4 0.0 0.0 0.8435776979217648 3.8915689844698926E-4 56-57 1.3734949356952563E-4 0.0 0.0 0.9934259954061173 3.8915689844698926E-4 58-59 1.3734949356952563E-4 0.0 0.0 1.1641399701219068 3.8915689844698926E-4 60-61 1.3734949356952563E-4 0.0 0.0 1.3445027467609556 4.234942718393707E-4 62-63 1.3734949356952563E-4 0.0 0.0 1.5283450439037658 4.349400629701645E-4 64-65 1.3734949356952563E-4 0.0 0.0 1.7226831315135136 4.463858541009583E-4 66-67 1.3734949356952563E-4 0.0 0.0 1.9497447359662012 4.578316452317521E-4 68-69 1.6024107583111324E-4 0.0 0.0 2.2070575663775767 4.578316452317521E-4 70-71 1.6024107583111324E-4 0.0 0.0 2.4889445103467662 4.578316452317521E-4 72-73 1.7168686696190705E-4 0.0 0.0 2.767100126407317 5.493979742781025E-4 74-75 1.8313265809270082E-4 0.0 0.0 3.063065393467383 5.493979742781025E-4 76-77 1.8313265809270082E-4 0.0 0.0 3.3814873027260672 5.493979742781025E-4 78-79 1.8313265809270082E-4 0.0 0.0 3.744238761034315 5.493979742781025E-4 80-81 2.0602424035428844E-4 0.0 0.0 4.1435366304231875 5.722895565396901E-4 82-83 2.0602424035428844E-4 0.0 0.0 4.542857391394321 5.951811388012777E-4 84-85 2.0602424035428844E-4 0.0 0.0 4.944925142236846 5.951811388012777E-4 86-87 2.0602424035428844E-4 0.0 0.0 5.3644248329715705 5.951811388012777E-4 88-89 2.0602424035428844E-4 0.0 0.0 5.822554068772723 5.951811388012777E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18025 0.0 24.593836 1 TACATGG 18535 0.0 23.197735 2 ACATGGG 18075 0.0 22.869139 3 GTATCAA 27795 0.0 21.16843 1 GAGTACT 12870 0.0 20.90235 12-13 GTACTTT 13520 0.0 19.792152 14-15 CATGGGG 10045 0.0 19.41788 4 AGTACTT 13815 0.0 19.077602 12-13 TACCTGG 3555 0.0 18.956524 2 GGTATCA 21770 0.0 18.789951 1 AGAGTAC 24070 0.0 18.449745 10-11 TCAACGC 32100 0.0 18.199646 4 ATCAACG 32050 0.0 18.198425 3 CAACGCA 32290 0.0 18.077858 5 TATCAAC 32600 0.0 17.935068 2 AACGCAG 32725 0.0 17.84736 6 CATGGGA 8470 0.0 16.809227 4 GTACCTG 4140 0.0 16.774057 1 ACTTTTT 16070 0.0 16.754564 16-17 ATGGGGA 6135 0.0 16.167456 5 >>END_MODULE