FastQCFastQC Report
Thu 26 May 2016
SRR938725_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938725_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4032065
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137620.3413139421115483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106790.26485188110806746No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75910.18826581416718233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70290.1743275468029409No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT41170.10210648885868655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG159900.025.0541861
GTATCAA253050.023.531811
TACATGG165700.023.4496752
ACATGGG162550.023.1170123
GAGTACT124550.021.31000512-13
GGTATCA191500.020.6475851
ATCAACG291050.020.2371353
TCAACGC291200.020.226714
CAACGCA293450.020.1200665
CGCGGAA5200.020.11690582-83
AGTACTT132700.020.0547412-13
TATCAAC295350.020.022722
AACGCAG299700.019.7327216
CATGGGG81150.019.4446984
GTACTTT136550.019.35070814-15
TACCTGG36550.019.317212
ATTCGCG5700.019.18514380-81
GGACCGT3759.094947E-1218.9396216
GTACCTG41200.018.8720321
AGAGTAC234000.018.11157210-11
Produced by FastQC (version 0.11.5)