##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938725_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4032065 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40572138593996 33.0 31.0 34.0 28.0 34.0 2 31.472306374029188 33.0 31.0 34.0 28.0 34.0 3 31.48164402111573 33.0 31.0 34.0 28.0 34.0 4 35.02283122915925 37.0 35.0 37.0 32.0 37.0 5 35.05147883280652 37.0 35.0 37.0 32.0 37.0 6 35.1338587051548 37.0 35.0 37.0 32.0 37.0 7 35.034561942825825 37.0 35.0 37.0 32.0 37.0 8 35.0036013804341 37.0 35.0 37.0 32.0 37.0 9 36.5427045446936 39.0 37.0 39.0 32.0 39.0 10-11 36.42312549524871 39.0 37.0 39.0 32.0 39.0 12-13 36.29091867318607 39.0 37.0 39.0 32.0 39.0 14-15 37.41368975946568 40.0 37.0 41.0 32.0 41.0 16-17 37.34181765422929 40.0 37.0 41.0 32.0 41.0 18-19 37.259750276843256 40.0 37.0 41.0 31.0 41.0 20-21 37.14416942683216 40.0 37.0 41.0 31.0 41.0 22-23 37.05776878596947 40.0 36.5 41.0 31.0 41.0 24-25 37.068336819966945 40.0 37.0 41.0 30.5 41.0 26-27 36.97161144475598 40.0 36.5 41.0 30.0 41.0 28-29 36.845976193340135 40.0 36.0 41.0 30.0 41.0 30-31 36.64581213348495 39.0 36.0 41.0 30.0 41.0 32-33 36.44776646705844 39.0 35.5 41.0 30.0 41.0 34-35 36.11714530891739 39.0 35.0 41.0 28.5 41.0 36-37 35.86708039180916 39.0 35.0 41.0 27.0 41.0 38-39 35.70560420032911 39.0 35.0 41.0 27.0 41.0 40-41 35.52635547790028 39.0 35.0 40.0 26.5 41.0 42-43 35.27504182100239 38.5 35.0 40.0 25.5 41.0 44-45 35.23764473042969 39.0 35.0 40.5 25.0 41.0 46-47 35.06399053586686 39.0 35.0 41.0 24.0 41.0 48-49 34.84058478719961 39.0 34.0 41.0 24.0 41.0 50-51 33.71096882118716 38.0 32.5 39.5 22.0 40.5 52-53 33.82034739023304 38.0 33.0 39.5 22.5 40.5 54-55 34.335815394841106 38.0 34.0 40.0 23.0 41.0 56-57 34.236759700054435 38.0 34.0 40.0 22.0 41.0 58-59 34.009644437775684 38.0 34.0 40.0 22.0 41.0 60-61 33.7085789787615 37.5 33.0 40.0 20.5 41.0 62-63 33.349939299093634 37.0 33.0 40.0 19.5 41.0 64-65 32.9507381453424 36.5 32.0 39.5 18.0 41.0 66-67 32.54732326983816 36.0 32.0 39.0 15.5 41.0 68-69 32.1425613178359 35.5 31.5 39.0 13.0 40.5 70-71 31.737070706945445 35.0 31.0 38.5 9.5 40.0 72-73 31.320124303551655 35.0 31.0 37.5 7.5 40.0 74-75 30.86873339095476 35.0 30.5 37.0 7.0 39.0 76-77 30.437467029921393 35.0 30.0 36.5 2.0 39.0 78-79 30.024301443553117 34.5 30.0 36.0 2.0 38.5 80-81 29.598749152109406 34.0 29.0 35.5 2.0 37.0 82-83 29.149582906029543 34.0 29.0 35.0 2.0 37.0 84-85 28.756905456633262 34.0 29.0 35.0 2.0 36.5 86-87 28.378443923895077 34.0 29.0 35.0 2.0 36.0 88-89 28.013560545278906 34.0 27.5 35.0 2.0 36.0 90-91 27.615998998032026 34.0 27.0 35.0 2.0 35.0 92-93 27.173086123363586 34.0 26.0 35.0 2.0 35.0 94-95 26.67991190618207 33.0 25.0 35.0 2.0 35.0 96-97 26.09949132764477 33.0 24.5 35.0 2.0 35.0 98-99 25.39705473498071 33.0 21.0 35.0 2.0 35.0 100-101 23.15025861438246 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.43195632057105726 1101 2 0.24515452071342736 1101 3 0.37384289487675915 1101 4 0.39254666844979624 1101 5 0.1353920290047128 1101 6 0.11008096413552693 1101 7 0.29457609596101975 1101 8 0.4182113771855427 1101 9 0.5047644438996031 1101 10-11 0.4259385002904921 1101 12-13 0.43168236052610354 1101 14-15 0.6574418176515877 1101 16-17 0.6404197094314625 1101 18-19 0.664180582526356 1101 20-21 0.708142065129131 1101 22-23 0.7091547154268412 1101 24-25 0.7372991050140953 1101 26-27 0.6509194932415241 1101 28-29 0.7009768668189977 1101 30-31 0.6375643162760625 1101 32-33 0.6734140699879774 1101 34-35 0.5571547537837986 1101 36-37 0.6416455604833331 1101 38-39 0.7213992408783341 1101 40-41 0.5707938641961618 1101 42-43 0.6153755424131759 1101 44-45 0.7511017616201698 1101 46-47 0.7737640063166182 1101 48-49 0.8414026107264405 1101 50-51 0.8542080241194228 1101 52-53 0.7340980636167771 1101 54-55 0.7315957666280895 1101 56-57 0.7498501624874407 1101 58-59 0.6440415801227175 1101 60-61 0.581474114462452 1101 62-63 0.6367839474511428 1101 64-65 0.7059158974342168 1101 66-67 0.5452018039189781 1101 68-69 0.5531044037996793 1101 70-71 0.48839717810316685 1101 72-73 0.47599637306174003 1101 74-75 0.5228298248489907 1101 76-77 0.4673064131034579 1101 78-79 0.27451690850969257 1101 80-81 0.2854425707492112 1101 82-83 0.16666573547154329 1101 84-85 0.16653155212017268 1101 86-87 0.16107413894781786 1101 88-89 0.03709861824492933 1101 90-91 0.04915081836601232 1101 92-93 -0.056452855844383976 1101 94-95 -0.08302030462045806 1101 96-97 -0.25548833911195956 1101 98-99 -0.11049153851891802 1101 100-101 0.03533248776429687 1102 1 0.31638141639576034 1102 2 0.32894784460703264 1102 3 0.3021347167706594 1102 4 0.24259982442389827 1102 5 0.24512595466312348 1102 6 0.37020442639504836 1102 7 0.2663967252115427 1102 8 0.2051080450480569 1102 9 0.3710793052635424 1102 10-11 0.3178110195523871 1102 12-13 0.39839329206828467 1102 14-15 0.27702836447728885 1102 16-17 0.3403377044935496 1102 18-19 0.3444328062102642 1102 20-21 0.39206002653737926 1102 22-23 0.38792393541631753 1102 24-25 0.39895909503100313 1102 26-27 0.35725561042207943 1102 28-29 0.38876324487596037 1102 30-31 0.4278313962889868 1102 32-33 0.3196046330048503 1102 34-35 0.38176278188054624 1102 36-37 0.43394376793005307 1102 38-39 0.266497388484332 1102 40-41 0.3374920506618224 1102 42-43 0.33951585446575905 1102 44-45 0.5126610004597438 1102 46-47 0.5523989856162288 1102 48-49 0.5684610786170623 1102 50-51 0.2970448112874209 1102 52-53 0.3719941058597058 1102 54-55 0.38206151734070204 1102 56-57 0.32200474692235304 1102 58-59 0.26335830467354526 1102 60-61 0.33691252866206156 1102 62-63 0.21600186117873932 1102 64-65 0.29940119800308906 1102 66-67 0.3968851662634094 1102 68-69 0.22072342368687003 1102 70-71 0.2867390101260874 1102 72-73 0.2114016171973283 1102 74-75 0.10073937989661275 1102 76-77 0.19481513253680305 1102 78-79 0.12292730365438942 1102 80-81 -0.053889372961062776 1102 82-83 -0.02809247550620597 1102 84-85 -0.06725696032629358 1102 86-87 -0.24884426495581735 1102 88-89 -0.13190579606063224 1102 90-91 -0.16330270548660408 1102 92-93 -0.1186843235237518 1102 94-95 -0.33727074491388365 1102 96-97 -0.33496066139373326 1102 98-99 -0.33977500492732915 1102 100-101 -0.23886613350632402 1103 1 0.123109833713265 1103 2 0.2676611450332018 1103 3 0.222458350012257 1103 4 0.35159655359342423 1103 5 0.23366637574687843 1103 6 0.28127415137034717 1103 7 0.21431114928859074 1103 8 0.21015928632952097 1103 9 0.4518699850826948 1103 10-11 0.38646039482635786 1103 12-13 0.37931301535758166 1103 14-15 0.4519170239555592 1103 16-17 0.5479012729929309 1103 18-19 0.6066874615020836 1103 20-21 0.5353258379290011 1103 22-23 0.4371274972754833 1103 24-25 0.4194862797563985 1103 26-27 0.35405708320363516 1103 28-29 0.4268866415594701 1103 30-31 0.46511257358044844 1103 32-33 0.4295154308955418 1103 34-35 0.45848807362254007 1103 36-37 0.5425754791631974 1103 38-39 0.45071161102011814 1103 40-41 0.5359837983643345 1103 42-43 0.4902497162504744 1103 44-45 0.47623215853086265 1103 46-47 0.4483889111471129 1103 48-49 0.5952422675807938 1103 50-51 0.5075399361677881 1103 52-53 0.5625332092734538 1103 54-55 0.47955042219610533 1103 56-57 0.5125018949968236 1103 58-59 0.47277160432156506 1103 60-61 0.45200575122118636 1103 62-63 0.4232393193206718 1103 64-65 0.3524874120103334 1103 66-67 0.3331732276025079 1103 68-69 0.23098769693219623 1103 70-71 0.349866686643292 1103 72-73 0.380460883815406 1103 74-75 0.3178308957099496 1103 76-77 0.32565256629664674 1103 78-79 0.3484213002149623 1103 80-81 0.14181879685235899 1103 82-83 0.2996039494060554 1103 84-85 0.3746152464337662 1103 86-87 0.36811217671264274 1103 88-89 0.2536279121688416 1103 90-91 0.3156785125281232 1103 92-93 0.32349713364289556 1103 94-95 0.1938435811160666 1103 96-97 0.22224340107627683 1103 98-99 0.1141275549319829 1103 100-101 0.3362977970929357 1104 1 0.10460725096908163 1104 2 0.18209783621051656 1104 3 0.134243691870072 1104 4 0.2628911932026483 1104 5 0.15844004260542732 1104 6 0.16793297258726625 1104 7 0.11354009133990672 1104 8 0.144817326405132 1104 9 0.16946678852993102 1104 10-11 0.13085765240770542 1104 12-13 0.23878703411263302 1104 14-15 0.24135074756172514 1104 16-17 0.28531873941470565 1104 18-19 0.23982990236445545 1104 20-21 0.2482517801168811 1104 22-23 0.36526294360457 1104 24-25 0.3336340316138475 1104 26-27 0.3770527282132079 1104 28-29 0.37929040609898124 1104 30-31 0.4078311431728636 1104 32-33 0.3478251534244521 1104 34-35 0.3272208186322345 1104 36-37 0.4168992558537852 1104 38-39 0.44301867741329914 1104 40-41 0.2656280791208161 1104 42-43 0.4458886438915144 1104 44-45 0.4321367635631006 1104 46-47 0.43517319999581616 1104 48-49 0.529908891276186 1104 50-51 0.49850706441408477 1104 52-53 0.44101710589220744 1104 54-55 0.5346910212498059 1104 56-57 0.5645455304182647 1104 58-59 0.46271198485990794 1104 60-61 0.46755738759735976 1104 62-63 0.3843398223399319 1104 64-65 0.3262339482485288 1104 66-67 0.3592062295464231 1104 68-69 0.376724254473082 1104 70-71 0.3285278717061324 1104 72-73 0.40812790063419513 1104 74-75 0.42437872976592317 1104 76-77 0.24676255916171286 1104 78-79 0.329104358510822 1104 80-81 0.31809261879043405 1104 82-83 0.27266385988113484 1104 84-85 0.34853789046946204 1104 86-87 0.4309001108874462 1104 88-89 0.549098217949922 1104 90-91 0.4256967136311651 1104 92-93 0.4022899448453785 1104 94-95 0.45835407865968136 1104 96-97 0.520092358724888 1104 98-99 0.5961420315736881 1104 100-101 0.6547161071983716 1105 1 0.20656440573539925 1105 2 0.21308149915605767 1105 3 0.2798095440809867 1105 4 0.2745071715613179 1105 5 0.324126532160669 1105 6 0.32647468757359377 1105 7 0.28001383602263985 1105 8 0.3084877807038282 1105 9 0.3325555501608761 1105 10-11 0.40432552446175407 1105 12-13 0.35992424894271124 1105 14-15 0.4656388081655223 1105 16-17 0.30210084352208355 1105 18-19 0.3923479952911535 1105 20-21 0.4367827669583093 1105 22-23 0.4577932406221734 1105 24-25 0.4113789056911088 1105 26-27 0.48418563856789376 1105 28-29 0.4326373261080576 1105 30-31 0.4157674213279492 1105 32-33 0.46784697476700643 1105 34-35 0.32375382459297697 1105 36-37 0.37436664578644496 1105 38-39 0.542815160507736 1105 40-41 0.49342885976722783 1105 42-43 0.4747349230127398 1105 44-45 0.5484663281464393 1105 46-47 0.5186474195618231 1105 48-49 0.5133243562322178 1105 50-51 0.5469621517545278 1105 52-53 0.47011819812373545 1105 54-55 0.49622450083501946 1105 56-57 0.4911898806133621 1105 58-59 0.42865300429487263 1105 60-61 0.5758780725873649 1105 62-63 0.4931633360166643 1105 64-65 0.4533766898512894 1105 66-67 0.3635884674645169 1105 68-69 0.3801991160284004 1105 70-71 0.38335080066907423 1105 72-73 0.335822280993348 1105 74-75 0.3983622388228518 1105 76-77 0.3223443383721474 1105 78-79 0.3918418512845072 1105 80-81 0.42384534653692896 1105 82-83 0.3355932571066731 1105 84-85 0.3734920149832881 1105 86-87 0.35001468174749206 1105 88-89 0.30745328398513294 1105 90-91 0.35486486856962074 1105 92-93 0.3125594450346725 1105 94-95 0.38455293862513074 1105 96-97 0.4010276364466314 1105 98-99 0.35292224118319737 1105 100-101 0.40720892966346867 1106 1 0.13646468125625333 1106 2 0.21884828666741996 1106 3 0.24449555031577574 1106 4 0.23670210075343334 1106 5 0.12951844977384752 1106 6 0.11275915646952228 1106 7 0.23797464725851825 1106 8 0.25667578858991646 1106 9 0.2754607382378538 1106 10-11 0.2785337372106724 1106 12-13 0.20805527862343354 1106 14-15 0.35842343023375633 1106 16-17 0.32795598195609443 1106 18-19 0.3907962383527277 1106 20-21 0.4284493416059121 1106 22-23 0.4481049265492274 1106 24-25 0.33348127527143134 1106 26-27 0.08751179651741126 1106 28-29 0.24687563066886042 1106 30-31 0.3095193162634189 1106 32-33 0.28375670311726253 1106 34-35 0.27564961189705883 1106 36-37 0.3612621633432056 1106 38-39 0.44751592554899133 1106 40-41 0.4383776913827475 1106 42-43 0.4698724988772014 1106 44-45 0.4723663506411526 1106 46-47 0.4508750307710656 1106 48-49 0.5058430169099708 1106 50-51 0.5509286993011955 1106 52-53 0.43759178260496157 1106 54-55 0.39642165299742516 1106 56-57 0.466452347908465 1106 58-59 0.3397063028944345 1106 60-61 0.3599416219004894 1106 62-63 0.25077262390583144 1106 64-65 0.3044521298154734 1106 66-67 0.09312116099717827 1106 68-69 0.3732256588489733 1106 70-71 0.26843241737359236 1106 72-73 0.23865488022486758 1106 74-75 0.16254301306390673 1106 76-77 0.078940638322333 1106 78-79 0.30939505066121953 1106 80-81 0.30144385715314215 1106 82-83 0.3724869921226812 1106 84-85 0.3118054411749007 1106 86-87 0.4681851134662445 1106 88-89 0.5382688074227069 1106 90-91 0.5863517315597555 1106 92-93 0.6831309591500307 1106 94-95 0.7347784063685587 1106 96-97 0.874007094951768 1106 98-99 0.8885212521727972 1106 100-101 1.216096267724371 1107 1 0.11735171575351089 1107 2 0.15645419491584178 1107 3 0.15958475647644477 1107 4 0.19267374062700782 1107 5 0.12274258889159029 1107 6 0.16390443753896733 1107 7 -0.07038269073748182 1107 8 0.14343572493964984 1107 9 0.1388790303292211 1107 10-11 0.13394017204928588 1107 12-13 0.2023077616836204 1107 14-15 0.3033015185795165 1107 16-17 0.2727608960421435 1107 18-19 0.4175723498662762 1107 20-21 0.4011886552829935 1107 22-23 0.37636742012522717 1107 24-25 0.30704786110845816 1107 26-27 0.3771484258641564 1107 28-29 0.24562877151023343 1107 30-31 0.3040109695803963 1107 32-33 0.44195825613702056 1107 34-35 0.5347441112897187 1107 36-37 0.5661503424319889 1107 38-39 0.4925351608704176 1107 40-41 0.5011829003069082 1107 42-43 0.5334793815649377 1107 44-45 0.5925324650268777 1107 46-47 0.7390073686238097 1107 48-49 0.747875476684122 1107 50-51 0.7047555713956797 1107 52-53 0.7264104282627599 1107 54-55 0.7227094869077249 1107 56-57 0.6963848267066695 1107 58-59 0.6450515314320668 1107 60-61 0.634824569848341 1107 62-63 0.5962156150176767 1107 64-65 0.5503064018288768 1107 66-67 0.7456550464414207 1107 68-69 0.7912596566679824 1107 70-71 0.8193189121949409 1107 72-73 0.6448200383074045 1107 74-75 0.5262837882740286 1107 76-77 0.438780759491852 1107 78-79 0.5610768149241068 1107 80-81 0.5400308294522738 1107 82-83 0.5184305971867182 1107 84-85 0.46136972372300633 1107 86-87 0.5670817312726122 1107 88-89 0.5313107350770387 1107 90-91 0.6825970419052005 1107 92-93 0.6918601697414566 1107 94-95 0.6058445106219814 1107 96-97 0.6971227428389888 1107 98-99 0.7494126393420224 1107 100-101 0.9050065764221422 1108 1 0.03922487636652505 1108 2 0.040281043261831684 1108 3 -0.12812754986907038 1108 4 -0.039327900736829235 1108 5 0.06651963982432108 1108 6 0.08060002478366357 1108 7 0.09013144018819474 1108 8 0.07166696888734947 1108 9 0.017505372689953447 1108 10-11 0.11066790776524016 1108 12-13 0.14188594218533268 1108 14-15 0.004186687016527912 1108 16-17 0.14450283622103655 1108 18-19 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84-85 0.5697497300279473 1108 86-87 0.4156433822498009 1108 88-89 0.44133100445273143 1108 90-91 0.5066417990350267 1108 92-93 0.5479839275962597 1108 94-95 0.6300766425206099 1108 96-97 0.6138545636184496 1108 98-99 0.7408229677646183 1108 100-101 1.1077768059715218 1109 1 0.27381426440338075 1109 2 0.22486515823382192 1109 3 0.22918523235973964 1109 4 0.33539398503899776 1109 5 0.2653173810350822 1109 6 0.27705684477400894 1109 7 0.25374601430031873 1109 8 0.2797037589011211 1109 9 0.3796303858726162 1109 10-11 0.3773892758219759 1109 12-13 0.3984817473823412 1109 14-15 0.3238133540240824 1109 16-17 0.283135067110976 1109 18-19 0.4740692722136757 1109 20-21 0.5324169964292196 1109 22-23 0.65445927551567 1109 24-25 0.579872159333874 1109 26-27 0.5294546922479171 1109 28-29 0.5823832552557846 1109 30-31 0.603302590942711 1109 32-33 0.5825783458999823 1109 34-35 0.742959783739046 1109 36-37 0.7339563091057997 1109 38-39 0.6807619085642145 1109 40-41 0.7207132916778747 1109 42-43 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-2.12177424793758 2316 62-63 -2.473534242236134 2316 64-65 -2.6301057705071926 2316 66-67 -2.3916503914012566 2316 68-69 -2.5840963372393375 2316 70-71 -2.449616733432375 2316 72-73 -2.550106324704899 2316 74-75 -2.5027539896507065 2316 76-77 -2.5469687530575555 2316 78-79 -2.512854299104088 2316 80-81 -2.4283684979601468 2316 82-83 -2.2263440915307164 2316 84-85 -2.2935459636937203 2316 86-87 -2.447129753593895 2316 88-89 -2.516605982204016 2316 90-91 -2.729088279363335 2316 92-93 -2.955377935260252 2316 94-95 -2.9437253497062805 2316 96-97 -2.8543335678935087 2316 98-99 -3.186551736046301 2316 100-101 -3.444248436090824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17271.0 3 6155.0 4 6363.0 5 6688.0 6 7647.0 7 8962.0 8 10856.0 9 12887.0 10 15208.0 11 18479.0 12 21841.0 13 26173.0 14 27648.0 15 26140.0 16 30502.0 17 36722.0 18 33014.0 19 26910.0 20 27883.0 21 30011.0 22 33148.0 23 36622.0 24 41725.0 25 47582.0 26 54606.0 27 62850.0 28 73940.0 29 86906.0 30 103248.0 31 124132.0 32 151985.0 33 191273.0 34 251056.0 35 349134.0 36 510548.0 37 746914.0 38 663802.0 39 105198.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.003070456544094 18.51969712778167 14.173212502422084 23.304019913252148 2 15.294938328763486 19.972369895847166 40.467924378415745 24.264767396973596 3 19.717454338656136 24.13538282201614 29.38979778414737 26.757365055180355 4 12.941679729841788 16.204949424781685 36.834057373365816 34.01931347201071 5 14.145081798882595 36.97240956133469 33.828688324913934 15.053820314868771 6 32.34420047140553 35.372534425009306 17.53447463093909 14.748790472646073 7 29.054484742034198 32.317698763944605 20.410781175990696 18.2170353180305 8 26.35430791810322 34.79419730310816 19.5605175431958 19.29097723559282 9 26.59548335325811 17.137591131662827 20.212855178382586 36.054070336696476 10-11 25.285602346651103 26.25580174351294 27.096046551904667 21.362549357931286 12-13 26.31561904438148 23.615579429689877 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38-39 22.618152221113025 26.42354302325089 28.624389031047276 22.333915724588813 40-41 22.66804704442988 26.37160407019652 28.537566724277884 22.422782161095714 42-43 22.57677009979889 26.485721802877766 28.200087102284392 22.737420995038956 44-45 22.62494468387081 26.5035771096874 28.099185806379595 22.772292400062195 46-47 22.762623736318226 26.70924153242108 27.788011055677686 22.74012367558301 48-49 22.977001454153058 26.698088168776458 27.66804688677917 22.656863490291315 50-51 22.552126466805404 26.90413559291625 27.813307939833553 22.730430000444795 52-53 22.863237666133163 27.015069967036588 27.483684949283944 22.638007417546305 54-55 22.524130295810497 26.662265314332682 27.725429870048806 23.088174519808017 56-57 22.699923654965286 27.050344997594056 27.494999705407068 22.75473164203359 58-59 22.95435448801684 26.64306021861017 27.536527705113762 22.866057588259228 60-61 22.81097313115455 26.554633775576075 28.08851660791331 22.545876485356064 62-63 22.663053931421977 26.614706295636715 27.97759160727935 22.744648165661957 64-65 22.516122849564976 26.772734585576313 27.787005788910214 22.9241367759485 66-67 22.484383949227084 26.87405071209989 27.549749290791276 23.091816047881743 68-69 22.37492070178762 26.76115565382233 27.792200670280216 23.071722974109836 70-71 22.5463205183202 26.730739655169405 27.593962735204393 23.128977091306 72-73 22.60482578965156 26.549320136501077 27.74862696384795 23.097227109999412 74-75 22.413084122397734 27.024855466263663 27.452425609798038 23.10963480154056 76-77 22.600021453658538 26.750295236976342 27.477801007474767 23.17188230189035 78-79 22.57107579894844 26.798786893981013 27.525297849064728 23.104839458005813 80-81 22.516351633294924 26.97356210880329 27.451611496901567 23.058474761000216 82-83 22.57365808179355 26.812925806928618 27.51114670649097 23.102269404786867 84-85 22.549653950229327 26.767302924537255 27.361874705541904 23.321168419691514 86-87 22.63179264559805 26.629554079595923 27.601738078060023 23.136915196746006 88-89 22.645024350098154 26.63858996827042 27.342085704121853 23.37429997750957 90-91 22.608638658155364 26.65917863503584 27.517419704380586 23.21476300242821 92-93 22.798249443028958 26.64408170212893 27.43116194271899 23.12650691212312 94-95 22.6715859451891 26.60510216263327 27.36925019757615 23.35406169460148 96-97 22.607051594069397 26.513997688631154 27.429896910556707 23.449053806742747 98-99 22.65098268885102 26.466911936499827 27.19731345422374 23.68479192042541 100-101 22.901166711706473 26.02336612954047 27.572262106021594 23.503205052731463 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3200.0 1 1878.5 2 759.5 3 1037.0 4 945.5 5 930.0 6 1623.0 7 3307.0 8 5257.0 9 6044.5 10 5313.0 11 4448.0 12 4592.5 13 5059.0 14 5394.5 15 5767.0 16 6165.5 17 6427.5 18 6589.5 19 6884.5 20 7351.5 21 8121.0 22 9328.0 23 10763.0 24 12644.5 25 15523.5 26 19561.0 27 24459.0 28 29369.0 29 34554.5 30 40785.5 31 48103.0 32 55167.0 33 61933.0 34 69708.0 35 76379.0 36 83351.5 37 91808.5 38 97672.0 39 101476.5 40 105876.5 41 114486.0 42 123768.5 43 131227.5 44 138607.0 45 144061.0 46 146929.5 47 154934.0 48 176825.0 49 179790.5 50 163906.0 51 155510.5 52 148570.0 53 145334.0 54 141011.0 55 130744.0 56 119277.0 57 107891.0 58 97018.5 59 85919.5 60 73082.0 61 59376.0 62 47901.0 63 38158.5 64 29578.5 65 23137.5 66 17619.0 67 14056.5 68 10470.0 69 7065.5 70 5291.0 71 4026.5 72 3167.5 73 2561.5 74 1959.5 75 1565.5 76 1137.5 77 681.5 78 428.5 79 267.0 80 176.0 81 134.5 82 106.0 83 89.0 84 63.5 85 38.0 86 25.0 87 29.0 88 24.0 89 14.0 90 14.0 91 14.5 92 11.0 93 6.0 94 7.0 95 5.5 96 1.5 97 2.0 98 3.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16381184331105775 2 0.09548457180129785 3 0.08799461318207916 4 0.10423939098204021 5 0.12534520152824918 6 0.03881385840754056 7 0.04325327096661388 8 0.04637822058920181 9 0.047295864525993506 10-11 0.044914950527831274 12-13 0.049465968430568455 14-15 0.0478166894630915 16-17 0.046043404558210245 18-19 0.050408413554841006 20-21 0.05286373111544581 22-23 0.06580995098045295 24-25 0.08274916202987799 26-27 0.08428683565369109 28-29 0.07400674344287604 30-31 0.06278420610778844 32-33 0.05188408420995197 34-35 0.29801106876997274 36-37 0.47262382922894347 38-39 0.45024075752747045 40-41 0.42712605079531213 42-43 0.4561682413353951 44-45 0.46549348782819716 46-47 0.4648362563599545 48-49 0.4816390608782349 50-51 0.4703421199807047 52-53 0.4782288975996171 54-55 0.46885404873185327 56-57 0.4476986358106826 58-59 0.4266176264519545 60-61 0.3963849789127904 62-63 0.45388653208715635 64-65 0.4916711412142414 66-67 0.4922291679325606 68-69 0.5037121177361972 70-71 0.5005375657386475 72-73 0.4907906990586709 74-75 0.48379676418906936 76-77 0.4653570812970525 78-79 0.4400102676916171 80-81 0.4449333034065671 82-83 0.4572222918033315 84-85 0.4547545736489863 86-87 0.46580350267170795 88-89 0.4773608560377871 90-91 0.48212268403411157 92-93 0.4839579719076949 94-95 0.48828577912310445 96-97 0.4891786218724153 98-99 0.4830651291583841 100-101 0.46994530098101095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4032065.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.1216108926627 #Duplication Level Percentage of deduplicated Percentage of total 1 72.43230450820693 35.57991478110999 2 14.399885103408385 14.146911058973535 3 5.368649483503984 7.911501328433319 4 2.5897063679154493 5.088421941239741 5 1.5087813706787818 3.7056885706290705 6 0.869911880968868 2.5638883756714232 7 0.5528075984572621 1.900835982494747 8 0.39304292305284644 1.5445521224253351 9 0.26805698895338376 1.1850651997584212 >10 1.4306215482809652 12.79877892929799 >50 0.11250878460226856 3.7838750290818908 >100 0.06800453396684986 6.092987182680556 >500 0.0037451256357446186 1.287840801047817 >1k 0.0017713431509644739 1.4386685209522805 >5k 1.0121960862654034E-4 0.36345833378818393 >10k+ 1.0121960862654034E-4 0.6076118424156637 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13762 0.3413139421115483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10679 0.26485188110806746 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7591 0.18826581416718233 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7029 0.1743275468029409 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4117 0.10210648885868655 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.720178122128487E-5 0.0 12-13 0.0 0.0 0.0 7.440356244256974E-5 0.0 14-15 0.0 0.0 0.0 7.440356244256974E-5 0.0 16-17 0.0 0.0 0.0 8.680415618299804E-5 0.0 18-19 0.0 0.0 0.0 1.1160534366385462E-4 0.0 20-21 0.0 0.0 0.0 2.1081009358728096E-4 0.0 22-23 0.0 0.0 0.0 4.464213746554185E-4 0.0 24-25 0.0 0.0 0.0 7.936379993874107E-4 0.0 26-27 0.0 0.0 0.0 0.0012648605615236857 0.0 28-29 0.0 0.0 0.0 0.0034721662473199215 0.0 30-31 0.0 0.0 0.0 0.010788516554172614 0.0 32-33 0.0 0.0 0.0 0.024863190449558724 0.0 34-35 0.0 0.0 0.0 0.04605580515195068 0.0 36-37 0.0 0.0 0.0 0.08346839646682283 0.0 38-39 0.0 0.0 0.0 0.13868824039295002 0.0 40-41 0.0 0.0 0.0 0.19687182622303956 0.0 42-43 0.0 0.0 0.0 0.25677909458304865 0.0 44-45 0.0 0.0 0.0 0.3314182683066865 0.0 46-47 0.0 0.0 0.0 0.4240755047351667 0.0 48-49 0.0 0.0 0.0 0.5272112428743088 0.0 50-51 0.0 0.0 0.0 0.6365844796648863 0.0 52-53 0.0 0.0 0.0 0.7441968321443231 0.0 54-55 0.0 0.0 0.0 0.8678183511426527 0.0 56-57 0.0 0.0 0.0 1.019428010213129 0.0 58-59 2.4801187480856583E-5 0.0 0.0 1.1866128150215833 0.0 60-61 2.4801187480856583E-5 0.0 0.0 1.3687775370684747 1.2400593740428291E-5 62-63 2.4801187480856583E-5 0.0 0.0 1.5526783422390265 2.4801187480856583E-5 64-65 2.4801187480856583E-5 0.0 0.0 1.7459539962773418 2.4801187480856583E-5 66-67 2.4801187480856583E-5 1.2400593740428291E-5 0.0 1.969772312698332 2.4801187480856583E-5 68-69 2.4801187480856583E-5 2.4801187480856583E-5 0.0 2.218863039162315 2.4801187480856583E-5 70-71 2.4801187480856583E-5 2.4801187480856583E-5 0.0 2.4951606682927983 2.4801187480856583E-5 72-73 2.4801187480856583E-5 2.4801187480856583E-5 0.0 2.7733059858906044 2.4801187480856583E-5 74-75 2.4801187480856583E-5 2.4801187480856583E-5 0.0 3.0590776686387744 2.4801187480856583E-5 76-77 2.4801187480856583E-5 2.4801187480856583E-5 0.0 3.3677532480255152 2.4801187480856583E-5 78-79 2.4801187480856583E-5 2.4801187480856583E-5 0.0 3.7205129381594793 2.4801187480856583E-5 80-81 2.4801187480856583E-5 2.4801187480856583E-5 0.0 4.106245807049241 2.4801187480856583E-5 82-83 2.4801187480856583E-5 2.4801187480856583E-5 0.0 4.500386278495014 2.4801187480856583E-5 84-85 2.4801187480856583E-5 2.4801187480856583E-5 0.0 4.903616385152521 2.4801187480856583E-5 86-87 2.4801187480856583E-5 2.4801187480856583E-5 0.0 5.325025762233496 2.4801187480856583E-5 88-89 2.4801187480856583E-5 2.4801187480856583E-5 0.0 5.781764430880951 2.4801187480856583E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15990 0.0 25.054186 1 GTATCAA 25305 0.0 23.53181 1 TACATGG 16570 0.0 23.449675 2 ACATGGG 16255 0.0 23.117012 3 GAGTACT 12455 0.0 21.310005 12-13 GGTATCA 19150 0.0 20.647585 1 ATCAACG 29105 0.0 20.237135 3 TCAACGC 29120 0.0 20.22671 4 CAACGCA 29345 0.0 20.120066 5 CGCGGAA 520 0.0 20.116905 82-83 AGTACTT 13270 0.0 20.05474 12-13 TATCAAC 29535 0.0 20.02272 2 AACGCAG 29970 0.0 19.732721 6 CATGGGG 8115 0.0 19.444698 4 GTACTTT 13655 0.0 19.350708 14-15 TACCTGG 3655 0.0 19.31721 2 ATTCGCG 570 0.0 19.185143 80-81 GGACCGT 375 9.094947E-12 18.939621 6 GTACCTG 4120 0.0 18.872032 1 AGAGTAC 23400 0.0 18.111572 10-11 >>END_MODULE