FastQCFastQC Report
Thu 26 May 2016
SRR938725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4032065
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133850.3319638944312654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117630.291736368337316No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82100.20361774921783254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77340.1918123839769448No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG161400.022.905231
GTATCAA262100.022.1389351
TACATGG165100.021.8147262
GAGTACT130900.021.64242612-13
GGTATCA201400.021.2892071
ACATGGG163950.021.0705093
TACCTGG34650.020.2681242
GTACTTT141600.019.8897214-15
ATCAACG301900.019.0499823
AGTACTT141100.019.03534112-13
TCAACGC302250.018.9965234
TATCAAC307300.018.8233182
CATGGGG84500.018.8123554
CAACGCA306500.018.7331125
AACGCAG312000.018.448516
GTACCTG41400.017.721351
GTATAGG10500.017.6950471
AGAGTAC250500.017.60789710-11
ACTTTTT165050.017.0779916-17
ACGCAGA339800.016.7297137
Produced by FastQC (version 0.11.5)