##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938725_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4032065 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00586250469672 33.0 31.0 34.0 30.0 34.0 2 32.23143500910824 34.0 31.0 34.0 30.0 34.0 3 32.3208770195917 34.0 31.0 34.0 30.0 34.0 4 35.944187159681206 37.0 35.0 37.0 35.0 37.0 5 35.85229752000526 37.0 35.0 37.0 35.0 37.0 6 35.88241409798701 37.0 35.0 37.0 35.0 37.0 7 35.817685478780724 37.0 35.0 37.0 33.0 37.0 8 35.78450024987196 37.0 35.0 37.0 33.0 37.0 9 37.44077687239665 39.0 37.0 39.0 34.0 39.0 10-11 37.36437532629063 39.0 37.0 39.0 34.0 39.0 12-13 37.28999272085147 39.0 37.0 39.0 34.0 39.0 14-15 38.51917640216614 40.0 38.0 41.0 34.0 41.0 16-17 38.447406477822156 40.0 38.0 41.0 33.0 41.0 18-19 38.462479151501775 40.0 38.0 41.0 33.5 41.0 20-21 38.54151507974202 40.0 38.0 41.0 34.0 41.0 22-23 38.50335721274335 40.0 38.0 41.0 34.0 41.0 24-25 38.43064558731072 40.0 38.0 41.0 34.0 41.0 26-27 38.27278379688819 40.0 38.0 41.0 33.5 41.0 28-29 38.117964988163635 40.0 38.0 41.0 33.0 41.0 30-31 37.907481278203605 40.0 37.0 41.0 33.0 41.0 32-33 37.70655408580963 40.0 37.0 41.0 32.5 41.0 34-35 37.68593971079335 40.0 37.0 41.0 33.0 41.0 36-37 37.61891202150759 40.0 37.0 41.0 32.5 41.0 38-39 37.52824842853476 40.0 37.0 41.0 32.0 41.0 40-41 37.38154432034206 40.0 36.5 41.0 31.5 41.0 42-43 37.216390732788284 40.0 36.0 41.0 31.0 41.0 44-45 36.98020790835466 40.0 36.0 41.0 31.0 41.0 46-47 36.71434537885674 39.5 36.0 41.0 30.0 41.0 48-49 36.45476263403492 39.0 35.5 41.0 30.0 41.0 50-51 36.22698406399698 39.0 35.0 41.0 29.5 41.0 52-53 36.02109688211871 39.0 35.0 41.0 29.0 41.0 54-55 35.77810179151378 39.0 35.0 40.0 28.0 41.0 56-57 35.5244266895499 38.0 35.0 40.0 28.0 41.0 58-59 35.22149320509466 38.0 34.0 40.0 27.5 41.0 60-61 34.87034125193914 38.0 34.0 40.0 27.0 41.0 62-63 34.50488471788029 37.0 34.0 40.0 26.0 41.0 64-65 34.13632307514884 36.5 33.0 39.5 26.0 41.0 66-67 33.7406109524524 36.0 33.0 39.0 26.0 40.5 68-69 33.29808795245116 35.5 32.5 39.0 25.0 40.0 70-71 32.81486893192446 35.0 32.0 38.0 24.0 40.0 72-73 32.452039339643584 35.0 32.0 37.5 23.0 39.5 74-75 32.02322085581457 35.0 31.0 37.0 22.0 39.0 76-77 29.983850334754028 33.0 29.0 35.0 19.5 37.5 78-79 31.052101838636034 34.0 30.5 36.0 20.0 38.0 80-81 31.103199229179094 34.0 31.0 36.0 20.0 37.0 82-83 30.799966766408776 34.0 31.0 35.0 19.5 37.0 84-85 30.413104575447072 34.0 30.5 35.0 18.0 36.5 86-87 29.874778184379466 34.0 30.0 35.0 11.5 36.0 88-89 29.357897628138435 34.0 29.0 35.0 7.0 36.0 90-91 28.938873877281246 34.0 29.0 35.0 2.0 35.0 92-93 28.651504502035557 34.0 29.0 35.0 2.0 35.0 94-95 28.256214619555983 34.0 29.0 35.0 2.0 35.0 96-97 27.713900941577084 33.5 28.0 35.0 2.0 35.0 98-99 26.949678886625094 33.0 26.5 35.0 2.0 35.0 100-101 23.716133420468175 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0904941829891115 1101 2 0.13140372354673246 1101 3 0.12444218494906067 1101 4 0.14780905182389148 1101 5 0.10503619320622448 1101 6 0.2050854484680258 1101 7 0.12472803820801914 1101 8 0.12040551761209173 1101 9 0.1759737619356656 1101 10-11 0.22408477509056013 1101 12-13 0.2523669107411095 1101 14-15 0.3041175439940105 1101 16-17 0.25650365386622553 1101 18-19 0.2782265279971057 1101 20-21 0.28006059571604425 1101 22-23 0.19595087731199357 1101 24-25 0.2404571837092817 1101 26-27 0.25015855974715606 1101 28-29 0.22295690633067977 1101 30-31 0.2639803152270943 1101 32-33 0.27451711420910385 1101 34-35 0.318972224891084 1101 36-37 0.34100526431034694 1101 38-39 0.41752826688736633 1101 40-41 0.4154770621571586 1101 42-43 0.3752874844813405 1101 44-45 0.38127192528917675 1101 46-47 0.39977764847183295 1101 48-49 0.33337304167475423 1101 50-51 0.4781057191650788 1101 52-53 0.40239718340558994 1101 54-55 0.34629782723389724 1101 56-57 0.22246618833180065 1101 58-59 0.26413755433828356 1101 60-61 0.26204683045367716 1101 62-63 0.18460977115032762 1101 64-65 0.15659057656475994 1101 66-67 0.060299427528619276 1101 68-69 0.09655139703338023 1101 70-71 0.16203688206915245 1101 72-73 0.12126666712592993 1101 74-75 0.2069006655955974 1101 76-77 0.1482467186914569 1101 78-79 0.15552484848733528 1101 80-81 0.10387878033852971 1101 82-83 0.1493862091813476 1101 84-85 0.21416863081888593 1101 86-87 0.36522936039526144 1101 88-89 0.49790561102442865 1101 90-91 0.496728813724463 1101 92-93 0.6094091146484821 1101 94-95 0.6096507975015335 1101 96-97 0.5022254553076131 1101 98-99 0.6653413593775248 1101 100-101 1.2788084296002147 1102 1 0.039665056275964616 1102 2 0.04299180477710962 1102 3 0.05893755287950597 1102 4 0.06917022073736945 1102 5 0.08356064068185276 1102 6 0.130580649575343 1102 7 0.08060233533906569 1102 8 0.12430725944798127 1102 9 0.14711079760308365 1102 10-11 0.17175729669244788 1102 12-13 0.15876319730301702 1102 14-15 0.22090336847163172 1102 16-17 0.2398766860205015 1102 18-19 0.202124617865735 1102 20-21 0.19955660583007528 1102 22-23 0.2074636449339451 1102 24-25 0.11357613464553395 1102 26-27 0.12006103854950823 1102 28-29 0.14823874736387666 1102 30-31 0.14239277824237462 1102 32-33 0.10921307741809727 1102 34-35 0.1577161137945069 1102 36-37 0.22705981060862968 1102 38-39 0.21249866794251915 1102 40-41 0.28149433025691195 1102 42-43 0.23984044911497193 1102 44-45 0.11949991794685388 1102 46-47 0.09283081930094994 1102 48-49 0.1775137047263442 1102 50-51 0.24113011596276124 1102 52-53 0.2711137259364804 1102 54-55 0.17546759362500097 1102 56-57 0.13639469022725592 1102 58-59 0.10767816683024733 1102 60-61 -0.032851037378506476 1102 62-63 -0.04462114400137551 1102 64-65 0.040092262345972074 1102 66-67 0.0027366082361908184 1102 68-69 0.049955094932869315 1102 70-71 0.0011729170607281958 1102 72-73 0.12350560043599046 1102 74-75 0.2585106993822137 1102 76-77 0.05398810288169997 1102 78-79 0.13159236860306223 1102 80-81 0.19729213925579003 1102 82-83 0.2276576087169495 1102 84-85 0.3387354198181285 1102 86-87 0.25995683595382957 1102 88-89 0.43103316735220787 1102 90-91 0.3931583387097426 1102 92-93 0.46208664554526635 1102 94-95 0.4246597584931848 1102 96-97 0.4247271738224434 1102 98-99 0.5297567179374774 1102 100-101 1.0567138699403067 1103 1 0.07659029406612206 1103 2 0.11218634007795458 1103 3 0.04796717192242994 1103 4 0.08347368429004831 1103 5 0.0994154790986741 1103 6 0.08449488170833064 1103 7 0.10887426818977275 1103 8 0.13321229642315302 1103 9 0.05965122906219733 1103 10-11 0.07903009588524412 1103 12-13 0.19508789661227866 1103 14-15 0.19839170268254946 1103 16-17 0.20728263991761509 1103 18-19 0.1684621696452382 1103 20-21 0.22679956290983228 1103 22-23 0.08474323998387234 1103 24-25 0.14869690377054923 1103 26-27 0.1749405677159288 1103 28-29 0.2723112555279883 1103 30-31 0.1954254943285818 1103 32-33 0.2669029010111359 1103 34-35 0.1254979797501221 1103 36-37 0.05826816328767137 1103 38-39 0.21892185530325747 1103 40-41 0.3088767061611932 1103 42-43 0.32994151803585936 1103 44-45 0.2796831985820347 1103 46-47 0.30583457517923307 1103 48-49 0.2557713066188114 1103 50-51 0.3542977375943366 1103 52-53 0.3406637468168867 1103 54-55 0.25439271988008727 1103 56-57 0.2487366930337842 1103 58-59 0.09630762142381144 1103 60-61 0.1732641316489847 1103 62-63 0.19902395143891027 1103 64-65 0.22076952657253202 1103 66-67 0.16441724704675664 1103 68-69 0.1321264101906081 1103 70-71 0.04573266483586025 1103 72-73 0.13665441673774126 1103 74-75 0.24579978788139556 1103 76-77 0.1249745267304867 1103 78-79 0.0944550845077039 1103 80-81 0.31059859609820606 1103 82-83 0.37139011651791876 1103 84-85 0.23720758403923625 1103 86-87 0.3132511406624232 1103 88-89 0.3966116322666515 1103 90-91 0.4840961151156655 1103 92-93 0.5332823273227731 1103 94-95 0.4073156676465821 1103 96-97 0.47712770659688886 1103 98-99 0.621893820464134 1103 100-101 1.1666234357576606 1104 1 0.03145034596351337 1104 2 0.023412566003933932 1104 3 0.027312749092679667 1104 4 -0.02501165463210242 1104 5 -0.01738046305091956 1104 6 0.020346715029113227 1104 7 -0.013741495803444082 1104 8 -0.0026592568865524413 1104 9 -0.04996676475759898 1104 10-11 -0.015760795649711667 1104 12-13 -0.11035619243272521 1104 14-15 -0.052474764274023755 1104 16-17 0.059053354521509505 1104 18-19 0.05120207384055675 1104 20-21 0.047144145784201896 1104 22-23 0.01923557471568671 1104 24-25 -0.08530128250101399 1104 26-27 -0.0881632053752881 1104 28-29 -0.0623057491014265 1104 30-31 -0.029158247976468488 1104 32-33 -0.07084371053316829 1104 34-35 -0.01059480120228784 1104 36-37 -0.07746972168288124 1104 38-39 0.09036721561745509 1104 40-41 0.05502243751750058 1104 42-43 0.07733283154028214 1104 44-45 0.07721054171877739 1104 46-47 0.034058187089847536 1104 48-49 -0.07646975843928061 1104 50-51 0.015761815943321267 1104 52-53 0.0772413267849501 1104 54-55 0.09503679769299822 1104 56-57 0.06352406815648237 1104 58-59 0.03047562934754211 1104 60-61 0.1820617037290546 1104 62-63 0.03888870677702272 1104 64-65 0.18494159336777471 1104 66-67 0.11616157260398552 1104 68-69 0.10310748921224189 1104 70-71 0.011193231878081633 1104 72-73 -0.06973728478459407 1104 74-75 -0.028912198170388592 1104 76-77 0.049362951799000854 1104 78-79 0.16481013365450892 1104 80-81 0.09237084873359791 1104 82-83 0.07274071239344337 1104 84-85 -0.0040407295507058905 1104 86-87 0.18013451447811235 1104 88-89 0.36259528956515297 1104 90-91 0.39327995008552463 1104 92-93 0.4091156404468421 1104 94-95 0.2742598607161213 1104 96-97 0.2715421314177817 1104 98-99 0.29984116107365466 1104 100-101 0.8493992379910615 1105 1 -0.02784894787971126 1105 2 -0.02834020643005175 1105 3 -0.0038533143453065577 1105 4 -0.024949280964740694 1105 5 -0.012415934533848372 1105 6 -0.0603581388821226 1105 7 -0.03442890900507223 1105 8 0.028061505142169096 1105 9 0.061611166293381814 1105 10-11 0.0743378409726958 1105 12-13 0.004692383184959681 1105 14-15 -0.01160238891672094 1105 16-17 0.011369283755406911 1105 18-19 -0.07683016912847762 1105 20-21 -0.02791699268313863 1105 22-23 -0.016253405569266022 1105 24-25 -0.029492679655426457 1105 26-27 -0.018879435714758586 1105 28-29 0.002359285108106235 1105 30-31 -0.03628864072602056 1105 32-33 0.01431686389621234 1105 34-35 -0.003712549909415941 1105 36-37 -0.01905231345038061 1105 38-39 -0.023884349152815787 1105 40-41 0.00705812582917531 1105 42-43 -0.014214088909369593 1105 44-45 0.108104829681821 1105 46-47 0.18899928614408168 1105 48-49 0.14372753998157606 1105 50-51 0.11475401860549539 1105 52-53 0.029712875486602286 1105 54-55 0.1625636251360305 1105 56-57 0.10042979926429751 1105 58-59 0.07226567177863075 1105 60-61 0.05280502349983607 1105 62-63 -0.08105711436034113 1105 64-65 0.053612452056796656 1105 66-67 0.05336488463943567 1105 68-69 0.06413294755012089 1105 70-71 0.03300403196355006 1105 72-73 -0.13704303502620263 1105 74-75 -0.05867963754252159 1105 76-77 -0.14479780924785857 1105 78-79 -0.24377099758730125 1105 80-81 -0.10513760333890687 1105 82-83 -0.05151065411542177 1105 84-85 0.010468417695523868 1105 86-87 -0.00276729608909676 1105 88-89 0.18627733114151468 1105 90-91 0.28584036395463386 1105 92-93 0.1457093458675267 1105 94-95 0.16191119632138395 1105 96-97 0.22767899850365225 1105 98-99 0.28581366316090495 1105 100-101 0.6800318523697975 1106 1 -0.0490890094262717 1106 2 -0.08683694721991486 1106 3 -0.027485387837721476 1106 4 -0.015355488875883339 1106 5 -0.04273313529494516 1106 6 -0.0794465948642511 1106 7 -0.14151140088977598 1106 8 -0.09166150379924431 1106 9 -0.06710171623907968 1106 10-11 -0.02505189910131378 1106 12-13 -0.015478879544822632 1106 14-15 -0.030331686598820795 1106 16-17 -0.13712093577473894 1106 18-19 -0.20030496889236105 1106 20-21 -0.09640552116577084 1106 22-23 -0.07770607217135961 1106 24-25 -0.15988260176943925 1106 26-27 -0.06218340813048684 1106 28-29 -0.07399059514617079 1106 30-31 -0.08136871782011923 1106 32-33 -0.13296965160888874 1106 34-35 -0.16311624112540812 1106 36-37 -0.21003246793514307 1106 38-39 -0.1483893312753537 1106 40-41 -0.13000159054808336 1106 42-43 -0.08344778252627094 1106 44-45 -0.08591618158352077 1106 46-47 -0.1680382320796312 1106 48-49 -0.2139635554859396 1106 50-51 -0.17801375608640058 1106 52-53 -0.09245363401149831 1106 54-55 -0.11456319049214869 1106 56-57 -0.1925522273614959 1106 58-59 -0.22114905478435531 1106 60-61 -0.2620353696939475 1106 62-63 -0.22676354157131584 1106 64-65 -0.26919952649087264 1106 66-67 -0.33939219480793525 1106 68-69 -0.23334836968754757 1106 70-71 -0.31713129916153093 1106 72-73 -0.15919944871825464 1106 74-75 0.010093056330873651 1106 76-77 -0.1144506996613579 1106 78-79 -0.15611785288568925 1106 80-81 -0.16090670973072108 1106 82-83 -0.01256245411876833 1106 84-85 -0.03946689027231187 1106 86-87 0.00202521458958671 1106 88-89 0.1175584920834929 1106 90-91 0.0832809509247987 1106 92-93 0.19144599739784596 1106 94-95 0.3023013298163342 1106 96-97 0.17308166815026382 1106 98-99 0.3080533357034376 1106 100-101 0.8152876375032818 1107 1 -0.061305332437353144 1107 2 -0.05999099899895555 1107 3 -0.09294731987369431 1107 4 0.0064158685148214545 1107 5 -0.06482174907156946 1107 6 -0.05177715284442996 1107 7 0.006454446000574876 1107 8 -0.044281296580066964 1107 9 -0.06507210660863905 1107 10-11 -0.07545844760561238 1107 12-13 -0.06985746162792594 1107 14-15 -0.0516224364592901 1107 16-17 0.02477428007075133 1107 18-19 0.016551546774834947 1107 20-21 -0.00873894818146681 1107 22-23 -0.0843428578792853 1107 24-25 0.017258458492840134 1107 26-27 -0.0603992345389841 1107 28-29 -0.06110169237530272 1107 30-31 -0.0742721055679354 1107 32-33 -0.01089382758455315 1107 34-35 -0.048207432814820095 1107 36-37 -0.029161283662645587 1107 38-39 0.07237640271757328 1107 40-41 -0.0058631906928141575 1107 42-43 -0.02628360373194738 1107 44-45 -0.08099039520740092 1107 46-47 0.002397654139052463 1107 48-49 -0.1204521412775037 1107 50-51 -0.03801285179864067 1107 52-53 -0.05052091037550355 1107 54-55 -0.12465938063842685 1107 56-57 -0.09135424130352732 1107 58-59 -0.13509836089148308 1107 60-61 -0.07324530733776413 1107 62-63 -0.13241021405372777 1107 64-65 -0.022752051786845584 1107 66-67 -0.08929117087838279 1107 68-69 0.06522915293050602 1107 70-71 0.012268644830562891 1107 72-73 0.051004821446902326 1107 74-75 0.08722370091108189 1107 76-77 0.09440586059050204 1107 78-79 0.08020244911115171 1107 80-81 0.033328978514440166 1107 82-83 0.0784449142521062 1107 84-85 0.014168740075763964 1107 86-87 0.09246400258958687 1107 88-89 0.19201286053327138 1107 90-91 0.3120388002338075 1107 92-93 0.34588512142009264 1107 94-95 0.37345413486216117 1107 96-97 0.4902383368221912 1107 98-99 0.478081977569655 1107 100-101 0.9022180912888551 1108 1 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-0.8777318872784861 2316 46-47 -0.8319504182127062 2316 48-49 -0.8549002694203764 2316 50-51 -0.83706230544108 2316 52-53 -0.8781432158879667 2316 54-55 -0.927777413897175 2316 56-57 -0.8343719071862594 2316 58-59 -0.7986384293464681 2316 60-61 -0.787019151616569 2316 62-63 -0.7533144388506727 2316 64-65 -0.7768792986979349 2316 66-67 -0.902161293689673 2316 68-69 -0.7852763118435675 2316 70-71 -0.9907426706034883 2316 72-73 -1.1545408121756822 2316 74-75 -1.151947770899362 2316 76-77 -1.0035504366871955 2316 78-79 -0.9582516747529901 2316 80-81 -0.9037910935553626 2316 82-83 -1.011828064616008 2316 84-85 -1.181621018128915 2316 86-87 -1.3287557618282726 2316 88-89 -1.599907236681215 2316 90-91 -2.0042121548120093 2316 92-93 -2.04033390924803 2316 94-95 -1.9910251223543263 2316 96-97 -2.0641114100259834 2316 98-99 -2.4907021735233066 2316 100-101 -3.2683107935564486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 45.0 5 107.0 6 260.0 7 646.0 8 1420.0 9 2500.0 10 3965.0 11 6251.0 12 8337.0 13 11516.0 14 14320.0 15 18251.0 16 23477.0 17 30830.0 18 30284.0 19 22389.0 20 21530.0 21 22450.0 22 24062.0 23 26970.0 24 31651.0 25 38202.0 26 46788.0 27 57292.0 28 70217.0 29 85396.0 30 104210.0 31 125893.0 32 154707.0 33 196667.0 34 258391.0 35 360592.0 36 545224.0 37 861854.0 38 748696.0 39 76658.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.629541586456824 19.48312331883 14.806893880018364 23.080441214694808 2 15.928240244142888 20.745970117049204 39.25968455369643 24.066105085111474 3 19.81009730745908 24.27262457326457 29.721891884183414 26.195386235092936 4 13.433339988318641 17.01775641017692 36.2006812886201 33.34822231288434 5 13.993375602823862 37.251805216433766 33.935812046680795 14.819007134061579 6 32.320585109615045 35.91839417271299 17.09325122486865 14.667769492803314 7 28.903824715127364 32.502650626912015 20.280377424471084 18.31314723348954 8 26.27482443859412 35.36827903320011 19.250235301266226 19.106661226939543 9 26.22152668669776 17.20659265165616 20.09466613261443 36.47721452903165 10-11 25.31856505289473 26.498543550265186 26.897743463957053 21.285147932883024 12-13 26.26089857182362 23.712601359353087 27.508212293204597 22.5182877756187 14-15 22.894647296565328 25.246648360042617 26.009236974883287 25.849467368508773 16-17 22.42422543881394 28.615345352335265 26.46548163165138 22.49494757719942 18-19 22.266858909200124 27.233985510382475 28.67776380780095 21.82139177261645 20-21 23.039249119247827 26.217136876513646 28.645631952857904 22.097982051380622 22-23 22.671373105344284 26.256248349170956 28.564966090576416 22.50741245490834 24-25 22.798789206027184 26.34442897125914 28.677464276989582 22.17931754572409 26-27 22.607637287143547 26.455261082424368 28.73443103525245 22.202670595179637 28-29 22.22648940431268 26.80849639080719 28.750788987776733 22.214225217103394 30-31 22.48827833876686 26.325170849180257 28.720977960424744 22.465572851628135 32-33 22.353843502026876 26.55828961090657 28.607537328887307 22.480329558179246 34-35 22.898726755108083 26.424767334053286 28.38412935735535 22.29237655348328 36-37 22.84790561578349 26.264873422117446 28.483607980647683 22.403612981451385 38-39 22.52945848379033 26.557332239311084 28.65010866637266 22.263100610525928 40-41 22.62503331381734 26.411181983540338 28.612566474164787 22.35121822847754 42-43 22.547280426049326 26.581554355826093 28.213404134870977 22.657761083253604 44-45 22.647974479087544 26.584643429574122 28.08133705771657 22.686045033621756 46-47 22.738205723316334 26.826848624428877 27.76927220698026 22.665673445274525 48-49 22.93917917235699 26.815307642028046 27.618109779741868 22.627403405873096 50-51 22.563309049549535 26.972608943449554 27.752547962002417 22.711534044998494 52-53 22.898192365450456 27.070793749604732 27.387839729766263 22.64317415517855 54-55 22.56182626024134 26.75048641328947 27.6171019068393 23.07058541962989 56-57 22.725774510083543 27.137558546303197 27.392055931638005 22.744611011975252 58-59 22.983105628819857 26.772862360688215 27.412871308983288 22.831160701508637 60-61 22.813557699438075 26.693726576689077 27.95360959871192 22.53910612516093 62-63 22.6383131224323 26.72594563827716 27.8128576796257 22.822883559664835 64-65 22.49499946057417 26.84845110383885 27.670598068235506 22.98595136735147 66-67 22.49148224183055 26.95242518168634 27.43769093264104 23.11840164384207 68-69 22.297242248299217 26.87708897332104 27.67584205938714 23.149826718992607 70-71 22.445377785488336 26.82109494057588 27.500932851900323 23.23259442203547 72-73 22.506432808002845 26.66683200791654 27.587117271174943 23.23961791290567 74-75 22.344158638315605 27.101634026237175 27.284059160752616 23.270148174694604 76-77 22.459972768809326 26.793366552456536 27.400811523741396 23.34584915499274 78-79 22.41392870363111 26.873152265710516 27.458199896194422 23.254719134463947 80-81 22.349362801453896 27.053281774952204 27.38102958943975 23.21632583415415 82-83 22.385737829127258 26.908680787636115 27.42691400064235 23.27866738259428 84-85 22.298524453350826 26.824431153763644 27.288399368561766 23.588645024323764 86-87 22.352722743073453 26.75529900061972 27.465918363038206 23.42605989326862 88-89 22.338675091517988 26.722991042851557 27.257822123844367 23.680511741786088 90-91 22.253911329720907 26.75012644069959 27.399858157598274 23.596104071981223 92-93 22.364297202550055 26.789783894852885 27.34874065770269 23.497178244894364 94-95 22.207147231895718 26.681654026119872 27.289109586416586 23.822089155567824 96-97 22.09435415707687 26.597035662132345 27.386990881097123 23.921619299693656 98-99 22.095677998479932 26.567094624565556 27.122580529955503 24.214646846999006 100-101 22.08852109643135 26.207820871024612 27.64101437868555 24.062643653858483 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1609.0 1 1082.5 2 701.5 3 873.5 4 774.5 5 756.0 6 1383.0 7 3020.5 8 4754.5 9 5361.0 10 4646.5 11 3809.5 12 3760.5 13 3996.5 14 4386.0 15 4651.5 16 4815.5 17 5061.0 18 5407.5 19 5811.0 20 6460.0 21 7452.5 22 8826.0 23 10513.0 24 12638.5 25 15450.5 26 19403.5 27 24379.0 28 29378.5 29 34792.0 30 41090.0 31 48515.0 32 55917.5 33 62855.5 34 70427.5 35 77158.5 36 84471.0 37 92255.0 38 98015.0 39 102277.5 40 106461.5 41 114750.0 42 124635.0 43 133014.5 44 140110.0 45 144805.5 46 147800.5 47 156327.5 48 179254.5 49 182647.0 50 165992.5 51 157085.0 52 149050.5 53 145451.5 54 141776.0 55 131739.0 56 119675.0 57 108034.0 58 97559.0 59 85907.5 60 72531.5 61 58609.0 62 46694.0 63 37082.5 64 28828.0 65 22517.0 66 16894.5 67 13174.0 68 9658.5 69 6437.0 70 4762.0 71 3616.0 72 2832.5 73 2317.5 74 1865.5 75 1579.5 76 1077.5 77 553.0 78 329.5 79 196.5 80 103.5 81 65.0 82 40.5 83 24.0 84 21.0 85 17.5 86 11.0 87 5.0 88 3.0 89 4.5 90 3.0 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3848400261404516 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.2321068732770923E-4 16-17 0.0021949050920558075 18-19 1.3640653114471122E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.2400593740428292E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00791157880639325 36-37 0.017683246673850742 38-39 0.011383745053713172 40-41 0.008171991274942244 42-43 0.0034101632786177802 44-45 0.0019220920297663853 46-47 0.0016616795612173912 48-49 1.2400593740428291E-5 50-51 4.712225621362751E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.9761424977027903E-4 60-61 5.952284995405581E-4 62-63 0.0 64-65 0.0 66-67 0.0011780564053406877 68-69 7.564362181661258E-4 70-71 0.0018476884673238154 72-73 0.0 74-75 0.0 76-77 0.09829950658037506 78-79 0.036036125409684615 80-81 3.720178122128488E-5 82-83 0.0 84-85 0.0 86-87 0.15054320800879945 88-89 0.33033941665126926 90-91 0.28286994381290975 92-93 0.0 94-95 1.2400593740428291E-5 96-97 2.4801187480856583E-5 98-99 2.3561128106813756E-4 100-101 5.0842434335756E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4032065.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.233530907624335 #Duplication Level Percentage of deduplicated Percentage of total 1 69.57275004124652 32.165938893603936 2 15.612111398177788 14.436060697218542 3 6.049901091301181 8.3912486727823 4 2.9294387545759744 5.417531888067235 5 1.6084705940481157 3.7182637461964223 6 0.9906151462814878 2.747978158989959 7 0.637607943048474 2.0635206579314884 8 0.43575346871546505 1.6117137171168778 9 0.3198887233183604 1.3310626658885916 >10 1.634615610103295 13.652589619216124 >50 0.1253617183617415 4.014493038334004 >100 0.07709187153562073 6.556589127643061 >500 0.004405701058815845 1.4434247914412228 >1k 0.0017730259864710393 1.4288434586832182 >5k 1.0745612039218382E-4 0.3960550078274923 >10k+ 1.0745612039218382E-4 0.6246858590595695 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13385 0.3319638944312654 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11763 0.291736368337316 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8210 0.20361774921783254 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7734 0.1918123839769448 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.4801187480856583E-5 0.0 10-11 0.0 0.0 0.0 4.9602374961713165E-5 0.0 12-13 0.0 0.0 0.0 7.440356244256974E-5 0.0 14-15 0.0 0.0 0.0 9.920474992342633E-5 0.0 16-17 0.0 0.0 0.0 1.1160534366385462E-4 0.0 18-19 0.0 0.0 0.0 1.3640653114471122E-4 0.0 20-21 0.0 0.0 0.0 1.984094998468527E-4 2.4801187480856583E-5 22-23 0.0 0.0 0.0 4.960237496171317E-4 2.4801187480856583E-5 24-25 0.0 0.0 0.0 8.680415618299805E-4 2.4801187480856583E-5 26-27 0.0 0.0 0.0 0.0013392641239662554 2.4801187480856583E-5 28-29 0.0 0.0 0.0 0.0035465698097624913 2.4801187480856583E-5 30-31 0.0 0.0 0.0 0.010875320710355612 2.4801187480856583E-5 32-33 0.0 0.0 0.0 0.025074000543146006 2.4801187480856583E-5 34-35 0.0 0.0 0.0 0.04648982593286567 2.4801187480856583E-5 36-37 0.0 1.2400593740428291E-5 0.0 0.08429923624743152 2.4801187480856583E-5 38-39 0.0 2.4801187480856583E-5 0.0 0.14023831461050354 2.4801187480856583E-5 40-41 0.0 2.4801187480856583E-5 0.0 0.1988187194402868 2.4801187480856583E-5 42-43 0.0 2.4801187480856583E-5 0.0 0.25919721036243215 2.4801187480856583E-5 44-45 0.0 2.4801187480856583E-5 0.0 0.334530817335534 2.4801187480856583E-5 46-47 0.0 2.4801187480856583E-5 0.0 0.4273864632638611 2.4801187480856583E-5 48-49 0.0 2.4801187480856583E-5 0.0 0.531067827527582 2.4801187480856583E-5 50-51 0.0 2.4801187480856583E-5 0.0 0.6411975005363257 2.4801187480856583E-5 52-53 0.0 2.4801187480856583E-5 0.0 0.7497150963588137 2.4801187480856583E-5 54-55 0.0 2.4801187480856583E-5 0.0 0.8740806509815691 2.4801187480856583E-5 56-57 0.0 2.4801187480856583E-5 0.0 1.026521149832654 2.4801187480856583E-5 58-59 0.0 2.4801187480856583E-5 0.0 1.1948716104527084 2.4801187480856583E-5 60-61 0.0 2.4801187480856583E-5 0.0 1.3783507954360856 2.4801187480856583E-5 62-63 0.0 2.4801187480856583E-5 0.0 1.563553662949382 2.4801187480856583E-5 64-65 0.0 2.4801187480856583E-5 0.0 1.7578957680493743 2.4801187480856583E-5 66-67 0.0 2.4801187480856583E-5 0.0 1.982309312969905 2.4801187480856583E-5 68-69 2.4801187480856583E-5 2.4801187480856583E-5 0.0 2.232181276839535 2.4801187480856583E-5 70-71 2.4801187480856583E-5 2.4801187480856583E-5 0.0 2.5102645914686397 2.4801187480856583E-5 72-73 2.4801187480856583E-5 2.4801187480856583E-5 0.0 2.7914852563140724 2.4801187480856583E-5 74-75 2.4801187480856583E-5 2.4801187480856583E-5 0.0 3.080791108278264 2.4801187480856583E-5 76-77 2.4801187480856583E-5 2.4801187480856583E-5 0.0 3.393162064599653 2.4801187480856583E-5 78-79 2.4801187480856583E-5 2.4801187480856583E-5 0.0 3.748389472887962 2.4801187480856583E-5 80-81 2.4801187480856583E-5 2.4801187480856583E-5 0.0 4.136825671213138 2.4801187480856583E-5 82-83 2.4801187480856583E-5 2.4801187480856583E-5 0.0 4.533123845969745 2.4801187480856583E-5 84-85 2.4801187480856583E-5 2.4801187480856583E-5 0.0 4.937742819126179 2.4801187480856583E-5 86-87 2.4801187480856583E-5 2.4801187480856583E-5 0.0 5.361756320892644 2.4801187480856583E-5 88-89 2.4801187480856583E-5 2.4801187480856583E-5 0.0 5.8211115148193295 2.4801187480856583E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16140 0.0 22.90523 1 GTATCAA 26210 0.0 22.138935 1 TACATGG 16510 0.0 21.814726 2 GAGTACT 13090 0.0 21.642426 12-13 GGTATCA 20140 0.0 21.289207 1 ACATGGG 16395 0.0 21.070509 3 TACCTGG 3465 0.0 20.268124 2 GTACTTT 14160 0.0 19.88972 14-15 ATCAACG 30190 0.0 19.049982 3 AGTACTT 14110 0.0 19.035341 12-13 TCAACGC 30225 0.0 18.996523 4 TATCAAC 30730 0.0 18.823318 2 CATGGGG 8450 0.0 18.812355 4 CAACGCA 30650 0.0 18.733112 5 AACGCAG 31200 0.0 18.44851 6 GTACCTG 4140 0.0 17.72135 1 GTATAGG 1050 0.0 17.695047 1 AGAGTAC 25050 0.0 17.607897 10-11 ACTTTTT 16505 0.0 17.07799 16-17 ACGCAGA 33980 0.0 16.729713 7 >>END_MODULE