FastQCFastQC Report
Thu 26 May 2016
SRR938724_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938724_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4047118
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137160.3389078351557825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107270.26505281041966156No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75260.18595949018536154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69280.17118354344993153No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43610.10775569182811078No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG164700.025.1581861
GTATCAA258350.023.9842171
TACATGG169150.023.9785882
ACATGGG168750.023.1358553
GAGTACT124050.022.78455512-13
GGTATCA193200.022.2094141
ATCAACG297200.020.6385523
TCAACGC297600.020.6108134
TATCAAC298600.020.5100142
CATGGGG88750.020.4719854
CAACGCA299750.020.4313285
GTACTTT137200.020.41061814-15
AACGCAG305050.020.10726
AGTACTT134850.019.4990912-13
ACGCAGA331450.018.3200597
AGAGTAC242250.018.03385710-11
CGCAGAG334900.018.0180118
CATGGGA86350.017.8546164
GTATAGG9350.017.7874161
GTGTAGC21200.017.707111
Produced by FastQC (version 0.11.5)