##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938724_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4047118 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.367944300116775 33.0 31.0 34.0 28.0 34.0 2 31.43501943852391 33.0 31.0 34.0 28.0 34.0 3 31.451650038372986 33.0 31.0 34.0 28.0 34.0 4 34.99122980847111 37.0 35.0 37.0 32.0 37.0 5 35.015916511453334 37.0 35.0 37.0 32.0 37.0 6 35.06481402321356 37.0 35.0 37.0 32.0 37.0 7 34.96602545317433 37.0 35.0 37.0 32.0 37.0 8 34.92857164036235 37.0 35.0 37.0 32.0 37.0 9 36.47503408598415 39.0 37.0 39.0 32.0 39.0 10-11 36.346704494408115 39.0 37.0 39.0 32.0 39.0 12-13 36.21571202025738 39.0 37.0 39.0 32.0 39.0 14-15 37.3426775547439 40.0 37.0 41.0 32.0 41.0 16-17 37.223792832331554 40.0 37.0 41.0 31.0 41.0 18-19 37.14768336381593 40.0 37.0 41.0 31.0 41.0 20-21 37.02384944051545 40.0 36.5 41.0 31.0 41.0 22-23 37.049229599927656 40.0 37.0 41.0 31.0 41.0 24-25 36.978883986085904 40.0 37.0 41.0 30.0 41.0 26-27 36.84344069038758 40.0 36.0 41.0 30.0 41.0 28-29 36.664932057824856 39.5 36.0 41.0 30.0 41.0 30-31 36.44742332692054 39.0 36.0 41.0 30.0 41.0 32-33 36.26927470362861 39.0 35.5 41.0 29.5 41.0 34-35 35.979348019998426 39.0 35.0 41.0 28.0 41.0 36-37 35.76561135603163 39.0 35.0 41.0 27.0 41.0 38-39 35.60248848686893 39.0 35.0 40.5 27.0 41.0 40-41 35.49862395907409 39.0 35.0 40.5 26.0 41.0 42-43 35.497953358414556 39.0 35.0 41.0 26.0 41.0 44-45 35.33190087365874 39.0 35.0 41.0 25.5 41.0 46-47 35.039992532958024 39.0 34.5 41.0 24.0 41.0 48-49 34.766537570686104 39.0 34.0 40.5 23.5 41.0 50-51 33.64488025800088 38.0 32.5 39.5 22.0 40.5 52-53 33.72433000470953 38.0 33.0 39.5 22.0 40.5 54-55 34.212185313104285 38.0 34.0 40.0 22.0 41.0 56-57 34.099929752480655 38.0 34.0 40.0 22.0 41.0 58-59 33.88166171581852 38.0 33.5 40.0 21.0 41.0 60-61 33.55685687938923 37.5 33.0 40.0 19.5 41.0 62-63 33.16120137836357 37.0 32.5 40.0 18.0 41.0 64-65 32.7574859443189 36.5 32.0 39.5 15.5 41.0 66-67 32.36155938621013 36.0 31.5 39.0 13.0 41.0 68-69 31.935140635879655 35.5 31.0 39.0 9.5 40.5 70-71 31.504602657990205 35.0 31.0 38.5 7.5 40.0 72-73 31.077017151464325 35.0 30.5 37.5 6.5 40.0 74-75 30.60766612191688 35.0 30.0 37.0 2.0 39.0 76-77 30.14285511319413 34.5 30.0 36.0 2.0 39.0 78-79 29.70665891135371 34.0 29.0 36.0 2.0 38.5 80-81 29.237114410798007 34.0 29.0 35.5 2.0 37.0 82-83 28.82163109649879 34.0 29.0 35.0 2.0 37.0 84-85 28.465162369864185 34.0 28.5 35.0 2.0 36.5 86-87 28.034031253845328 34.0 27.0 35.0 2.0 36.0 88-89 27.57995146175624 34.0 26.5 35.0 2.0 36.0 90-91 27.12600596276165 33.0 26.0 35.0 2.0 35.0 92-93 26.650605072547922 33.0 25.0 35.0 2.0 35.0 94-95 26.11993411113785 33.0 24.0 35.0 2.0 35.0 96-97 25.49248489913069 32.5 21.5 35.0 2.0 35.0 98-99 24.692863538943023 32.0 10.0 35.0 2.0 35.0 100-101 22.16768278068492 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11693715024048501 1101 2 0.2132960678985505 1101 3 0.3499829308124909 1101 4 0.20784756886090605 1101 5 0.1660719471453902 1101 6 0.2763122141794554 1101 7 0.36191431719882416 1101 8 0.44487782158093125 1101 9 0.4704542715698494 1101 10-11 0.3650981809784142 1101 12-13 0.3273504119053996 1101 14-15 0.39665308487468565 1101 16-17 0.624997386409845 1101 18-19 0.5728273262430932 1101 20-21 0.7087614183341415 1101 22-23 0.614282961763088 1101 24-25 0.6779093953785704 1101 26-27 0.5867091848030981 1101 28-29 0.6700930813612658 1101 30-31 0.7175435670188577 1101 32-33 0.5574393086731035 1101 34-35 0.5265928085336711 1101 36-37 0.5449635914333157 1101 38-39 0.4995367641458657 1101 40-41 0.5401021325625877 1101 42-43 0.5966495971253565 1101 44-45 0.7576739774402625 1101 46-47 0.6971883834397872 1101 48-49 0.5715699356937236 1101 50-51 0.6071175850531318 1101 52-53 0.621322911118213 1101 54-55 0.6693650304933882 1101 56-57 0.6762333560433191 1101 58-59 0.6307466916558582 1101 60-61 0.5334961417870545 1101 62-63 0.4958972072168919 1101 64-65 0.5618452893098649 1101 66-67 0.5020682826471301 1101 68-69 0.6022132485407781 1101 70-71 0.541743929046671 1101 72-73 0.5234656473454464 1101 74-75 0.46573081804579175 1101 76-77 0.3362973034673402 1101 78-79 0.2934610662520072 1101 80-81 0.36460490922841515 1101 82-83 0.35444583556150633 1101 84-85 0.2666849800348423 1101 86-87 0.2462405188888006 1101 88-89 0.2961501439582257 1101 90-91 0.32419196648147164 1101 92-93 0.3600356248174883 1101 94-95 0.22695315340905253 1101 96-97 0.1622744247857888 1101 98-99 0.11186449254370245 1101 100-101 0.4029884225656417 1102 1 0.08569045706880019 1102 2 0.09821779459742075 1102 3 0.12574222487039677 1102 4 0.19278684970846882 1102 5 0.18495805157128586 1102 6 0.25205355302728094 1102 7 0.23863322776799833 1102 8 0.29712186805592466 1102 9 0.4490190826282827 1102 10-11 0.45068897063436 1102 12-13 0.37868744261344034 1102 14-15 0.5083239714270604 1102 16-17 0.6396557792828048 1102 18-19 0.6732934212841641 1102 20-21 0.5559073266286774 1102 22-23 0.5718570524566431 1102 24-25 0.5946110109464158 1102 26-27 0.5993781589096514 1102 28-29 0.5465154841570978 1102 30-31 0.586004839450915 1102 32-33 0.616362446491074 1102 34-35 0.5982216638520086 1102 36-37 0.7271806217958172 1102 38-39 0.6522583513370748 1102 40-41 0.6385674557147212 1102 42-43 0.7505449367611163 1102 44-45 0.7140364308130245 1102 46-47 0.6330104775417524 1102 48-49 0.5139983439211662 1102 50-51 0.6303424333362315 1102 52-53 0.6314790196380216 1102 54-55 0.6487505057156611 1102 56-57 0.6280124181088027 1102 58-59 0.51604189906476 1102 60-61 0.4774916819843611 1102 62-63 0.44853697788381197 1102 64-65 0.5580408209306285 1102 66-67 0.5352962499538378 1102 68-69 0.6658868351618636 1102 70-71 0.5531606043772861 1102 72-73 0.44326157496805507 1102 74-75 0.409481001222872 1102 76-77 0.5163037759812141 1102 78-79 0.4661634086665032 1102 80-81 0.49390299934320936 1102 82-83 0.4780582325542895 1102 84-85 0.3756228491462963 1102 86-87 0.3975200435631798 1102 88-89 0.4714743481058825 1102 90-91 0.3418245997981373 1102 92-93 0.24989330663944287 1102 94-95 0.21660802156937464 1102 96-97 0.21196050169270464 1102 98-99 0.07220942385268359 1102 100-101 0.3513972114867201 1103 1 0.24892403654800788 1103 2 0.3684309510187944 1103 3 0.3859316389852516 1103 4 0.36743475511124046 1103 5 0.42023121282310427 1103 6 0.3129001082676339 1103 7 0.3630878882453814 1103 8 0.4400724955259818 1103 9 0.5021614522464617 1103 10-11 0.5110205128452989 1103 12-13 0.5071867657279228 1103 14-15 0.5314407968591439 1103 16-17 0.6779273747279007 1103 18-19 0.7469712240227864 1103 20-21 0.6642182116464213 1103 22-23 0.6054856897967156 1103 24-25 0.5940604568109222 1103 26-27 0.6411259106622254 1103 28-29 0.6184452033454235 1103 30-31 0.7889258158298773 1103 32-33 0.7310258652536348 1103 34-35 0.816224420800431 1103 36-37 0.9054173615272063 1103 38-39 0.9155823759683344 1103 40-41 0.8454481775224991 1103 42-43 0.9600702922524036 1103 44-45 1.0402337922098184 1103 46-47 0.9868506758889808 1103 48-49 0.9053099179035442 1103 50-51 0.9236400528026323 1103 52-53 0.834268094314119 1103 54-55 0.8596678374056665 1103 56-57 0.9391996260715629 1103 58-59 0.9481311705731983 1103 60-61 0.8925434433367911 1103 62-63 0.8345268010180646 1103 64-65 0.8646845192836352 1103 66-67 0.7855515616387407 1103 68-69 0.8717491559403854 1103 70-71 0.8380088253875009 1103 72-73 0.8539938809918723 1103 74-75 0.6489328156871537 1103 76-77 0.8022481404060322 1103 78-79 0.7532538375314033 1103 80-81 0.7500470545301319 1103 82-83 0.8359852948178883 1103 84-85 0.8625217132658669 1103 86-87 0.8670664924284566 1103 88-89 0.8797661850282701 1103 90-91 0.7633037612968607 1103 92-93 0.6151238542867183 1103 94-95 0.5895017342266193 1103 96-97 0.5346552780038252 1103 98-99 0.8565895557810279 1103 100-101 1.30556429113458 1104 1 0.08204483504924553 1104 2 0.18722851222215553 1104 3 0.09212804184073065 1104 4 0.23221260784004727 1104 5 0.1968023031105659 1104 6 0.21866191134176916 1104 7 0.31372182130309767 1104 8 0.3320495790192979 1104 9 0.36775399178713286 1104 10-11 0.3418880034765195 1104 12-13 0.3077215939797071 1104 14-15 0.44139293716362005 1104 16-17 0.5194731933799801 1104 18-19 0.47065911737296773 1104 20-21 0.5233562600202362 1104 22-23 0.3516259322606814 1104 24-25 0.2893527471865056 1104 26-27 0.4067977857241942 1104 28-29 0.4191849050803995 1104 30-31 0.48988377887970813 1104 32-33 0.43671821143988865 1104 34-35 0.5043512768748641 1104 36-37 0.5503504037413052 1104 38-39 0.5503539758346463 1104 40-41 0.6126027188877075 1104 42-43 0.7446332510545304 1104 44-45 0.770270690467953 1104 46-47 0.8139606804809816 1104 48-49 0.7586459215925316 1104 50-51 0.9421396265912847 1104 52-53 0.8340038528880029 1104 54-55 0.7826941447352382 1104 56-57 0.7410078306010988 1104 58-59 0.7623754569194503 1104 60-61 0.7376884735729732 1104 62-63 0.6983684068656473 1104 64-65 0.7739314249098683 1104 66-67 0.7350525764820475 1104 68-69 0.7375797981873617 1104 70-71 0.7707127683923538 1104 72-73 0.644579260926875 1104 74-75 0.5648889898871232 1104 76-77 0.6261613495209595 1104 78-79 0.454425927772121 1104 80-81 0.5826564054137826 1104 82-83 0.5665039867666408 1104 84-85 0.6008878197467205 1104 86-87 0.5436615762580352 1104 88-89 0.5532309919160099 1104 90-91 0.7105030966162573 1104 92-93 0.7490498515449744 1104 94-95 0.7768828556293741 1104 96-97 0.6042100508799813 1104 98-99 0.6702694861354601 1104 100-101 1.0022208724616348 1105 1 0.16624775462072705 1105 2 0.201910363591832 1105 3 0.224399796890701 1105 4 0.2356826411953037 1105 5 0.2435924403116232 1105 6 0.33968520711019323 1105 7 0.2863721590758814 1105 8 0.29882911951907687 1105 9 0.361998269164566 1105 10-11 0.3872184310285931 1105 12-13 0.40934041218529416 1105 14-15 0.5252545837191462 1105 16-17 0.6009999542065145 1105 18-19 0.5275763506037592 1105 20-21 0.5910575859307272 1105 22-23 0.5273331292025674 1105 24-25 0.5702667091598883 1105 26-27 0.52210794117331 1105 28-29 0.6356706715870288 1105 30-31 0.6449984958973332 1105 32-33 0.6817214195993913 1105 34-35 0.6899087995084585 1105 36-37 0.8224241332287789 1105 38-39 0.7267124917860599 1105 40-41 0.7821451982325129 1105 42-43 0.7267977630859193 1105 44-45 0.7150709142021014 1105 46-47 0.8330426739204313 1105 48-49 0.857491475842032 1105 50-51 0.9667809665331504 1105 52-53 1.0411817424938477 1105 54-55 0.9028642433306899 1105 56-57 0.9119099292325927 1105 58-59 0.8930754713535336 1105 60-61 0.9176623587682826 1105 62-63 0.9330465917673152 1105 64-65 0.9439220866546734 1105 66-67 0.8496699719997096 1105 68-69 0.8179335994729868 1105 70-71 0.8125593489485361 1105 72-73 0.8824013660531307 1105 74-75 0.880928610920428 1105 76-77 0.767025510978538 1105 78-79 0.8162804780965587 1105 80-81 0.9518264491058588 1105 82-83 0.8212554986871723 1105 84-85 0.8181661731513898 1105 86-87 0.8682376760140578 1105 88-89 0.9621811013774071 1105 90-91 0.9737130928690156 1105 92-93 1.0348726019321397 1105 94-95 1.120670568770631 1105 96-97 1.1283962723353511 1105 98-99 1.134754118518778 1105 100-101 1.6660211794288244 1106 1 0.11253467604998946 1106 2 0.088747276883403 1106 3 0.19465281366503362 1106 4 0.20100493411099052 1106 5 0.2621632639789624 1106 6 0.24712321536753024 1106 7 0.3079802008200119 1106 8 0.23611755236368737 1106 9 0.18912159503806691 1106 10-11 0.21195160179009775 1106 12-13 0.23086073065047685 1106 14-15 0.31194371063880055 1106 16-17 0.44301585062457605 1106 18-19 0.37137153511550736 1106 20-21 0.40858528962007057 1106 22-23 0.37713797817420414 1106 24-25 0.25434729401814593 1106 26-27 0.14479144324856463 1106 28-29 0.15973679626417692 1106 30-31 -0.006490610459159996 1106 32-33 0.21542718164150187 1106 34-35 0.25887487267533515 1106 36-37 0.3902820360748791 1106 38-39 0.4080000745803787 1106 40-41 0.5475869633618231 1106 42-43 0.5597257188787594 1106 44-45 0.5693466023203086 1106 46-47 0.5166361346976203 1106 48-49 0.6622296702483297 1106 50-51 0.5511845966595956 1106 52-53 0.41156866436666917 1106 54-55 0.4245199113201963 1106 56-57 0.5912485891989476 1106 58-59 0.6656825553409789 1106 60-61 0.6420915859401788 1106 62-63 0.6653970445038198 1106 64-65 0.6597758884687508 1106 66-67 0.715207347882874 1106 68-69 0.7064653246940651 1106 70-71 0.7710734203288716 1106 72-73 0.8569225474205844 1106 74-75 0.6943519274335941 1106 76-77 0.7277298209746093 1106 78-79 0.8797279347185736 1106 80-81 0.8544272515469622 1106 82-83 0.7547568346673295 1106 84-85 0.8243450174082803 1106 86-87 0.8965303385241903 1106 88-89 1.139107285871983 1106 90-91 1.2537039224710114 1106 92-93 1.3274225719574595 1106 94-95 1.3747585497715384 1106 96-97 1.355789074613586 1106 98-99 1.512108859607725 1106 100-101 1.9383164039181864 1107 1 -0.015645537798242515 1107 2 0.012338928329306498 1107 3 0.09886193714541136 1107 4 0.06975991768378975 1107 5 -0.0034522300575829945 1107 6 -0.02753074722165394 1107 7 0.010434433314735259 1107 8 0.026567441679972603 1107 9 0.04924930917388082 1107 10-11 0.013094884593300549 1107 12-13 0.09927723809532552 1107 14-15 0.15173092856366566 1107 16-17 0.24231810834788803 1107 18-19 0.1771798909643536 1107 20-21 0.06859452027708102 1107 22-23 0.12213667353726976 1107 24-25 0.13375327739174026 1107 26-27 0.11689516328750926 1107 28-29 0.09981563561427009 1107 30-31 -0.08412571052340923 1107 32-33 0.11547855347424729 1107 34-35 0.2104562609773737 1107 36-37 0.13761591931969264 1107 38-39 -0.0192091246531092 1107 40-41 0.0036352040659295426 1107 42-43 0.12413743984918568 1107 44-45 0.3708166054907167 1107 46-47 0.4071991486614408 1107 48-49 0.2500387499602468 1107 50-51 0.3592616459917082 1107 52-53 0.24792176926228393 1107 54-55 0.1566173747132069 1107 56-57 0.3263344006410378 1107 58-59 0.38468505519367824 1107 60-61 0.3299578732801507 1107 62-63 0.28547038473910646 1107 64-65 0.26453986141086716 1107 66-67 0.1605302665661199 1107 68-69 0.344699940499531 1107 70-71 0.3908551671956424 1107 72-73 0.23598522891241203 1107 74-75 0.2771976351780836 1107 76-77 0.21035623748766952 1107 78-79 0.2856455153325115 1107 80-81 0.18140756415480652 1107 82-83 -0.00898730498774114 1107 84-85 0.29125151281679607 1107 86-87 0.08746701172806937 1107 88-89 0.2462452491233087 1107 90-91 0.4494361885271623 1107 92-93 0.5122286860753142 1107 94-95 0.4387075875667321 1107 96-97 0.6274543524731975 1107 98-99 0.8680240554014631 1107 100-101 1.260436396847897 1108 1 0.23792167295401256 1108 2 0.28634580420329314 1108 3 0.14548677456091497 1108 4 0.24455080196026557 1108 5 0.2561595822786842 1108 6 0.2909549728215808 1108 7 0.31213605946503975 1108 8 0.3320718658102564 1108 9 0.36645682500487453 1108 10-11 0.3888649702669298 1108 12-13 0.44592292907181275 1108 14-15 0.526172645455965 1108 16-17 0.6450909907802114 1108 18-19 0.6275530262041187 1108 20-21 0.6262309538601656 1108 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11 18160.0 12 20988.0 13 24432.0 14 25907.0 15 27600.0 16 31860.0 17 37251.0 18 35098.0 19 28930.0 20 29763.0 21 32128.0 22 35228.0 23 39033.0 24 44147.0 25 50345.0 26 57070.0 27 66106.0 28 76814.0 29 90111.0 30 106694.0 31 128046.0 32 156312.0 33 196385.0 34 255748.0 35 353847.0 36 512619.0 37 737072.0 38 633069.0 39 93280.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02527613405876 18.486202093113263 14.219547451845957 23.268974320982025 2 15.322024166043787 19.948643444428495 40.412089951935215 24.317242437592505 3 19.69402735656372 24.120418394503933 29.363668997957543 26.82188525097481 4 12.912098111066946 16.17715411181141 36.83107609438676 34.07967168273488 5 14.095366649535922 36.99222924651554 33.81809625827384 15.094307845674692 6 32.378384668137265 35.31448292594766 17.548630040330067 14.758502365585008 7 29.071053680985393 32.28237689386903 20.439543564250986 18.207025860894593 8 26.303598635955943 34.79082700930095 19.569355626161567 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26.473114840299196 28.58852106989067 22.5884705669157 34-35 22.972406642759847 26.368019052715603 28.321423319589517 22.338150984935034 36-37 22.896625752450987 26.14441824658996 28.471848648865876 22.48710735209317 38-39 22.61170343506556 26.420876821337252 28.619167981816823 22.34825176178036 40-41 22.683000663455502 26.339728885702186 28.572792175786947 22.40447827505536 42-43 22.536008052480508 26.4772543095019 28.20444044647765 22.782297191539936 44-45 22.604581816594386 26.529822832985587 28.0908485701728 22.77474678024722 46-47 22.7779230237263 26.73385628489417 27.757550832499067 22.73066985888047 48-49 22.94704492787368 26.69134659076037 27.6394020202341 22.72220646113185 50-51 22.57747814137056 26.86459638207727 27.813551587407414 22.744373889144757 52-53 22.82774732959024 26.9935431374361 27.50664385171605 22.672065681257607 54-55 22.53762064498204 26.607465895679884 27.736286798153646 23.118626661184425 56-57 22.692303924468153 27.02559125200376 27.51825010314103 22.76385472038705 58-59 22.96770531964232 26.6377339932135 27.53451298009388 22.860047707050295 60-61 22.830783240610764 26.547439953610418 28.071385824334033 22.55039098144479 62-63 22.682067298724903 26.605825439219622 27.94490209579294 22.767205166262535 64-65 22.5110755671828 26.785994523345874 27.77626082698643 22.926669082484892 66-67 22.50906106139289 26.85935064068224 27.513754426936714 23.117833870988154 68-69 22.351828186453925 26.726100820109433 27.792677328502833 23.129393664933808 70-71 22.534088860506923 26.682097810007605 27.61017487705479 23.173638452430684 72-73 22.63875037171675 26.529652420491693 27.718513311646703 23.11308389614485 74-75 22.465687353290935 26.96066330050954 27.47207488082777 23.101574465371762 76-77 22.606667960758024 26.729196499503555 27.45536780205612 23.208767737682308 78-79 22.5653141557322 26.76309283954879 27.520271791804184 23.151321212914823 80-81 22.532188601686293 26.942660443224902 27.457933542680212 23.067217412408596 82-83 22.630983872344057 26.763224400050007 27.51358358618582 23.092208141420116 84-85 22.582731640220697 26.73937215548457 27.35901838908103 23.318877815213703 86-87 22.6797694949964 26.627919263717487 27.56964164171315 23.122669599572962 88-89 22.657360976206935 26.59953286323629 27.361287532216277 23.381818628340497 90-91 22.644716027814695 26.606390988497054 27.488220215017904 23.260672768670343 92-93 22.83108880539754 26.618172209289128 27.38697497678556 23.163764008527778 94-95 22.694176654459067 26.538479003613645 27.338875430117547 23.428468911809745 96-97 22.686567127143352 26.39114161683508 27.440871138529978 23.48142011749159 98-99 22.707048184602122 26.41289180687947 27.17267547944595 23.707384529072456 100-101 22.98613272216414 25.88039657365876 27.587663216767393 23.545807487409707 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4269.0 1 2813.5 2 1369.5 3 1417.0 4 1320.0 5 1390.5 6 2096.0 7 3649.0 8 5447.5 9 6068.5 10 5247.5 11 4341.5 12 4260.5 13 4558.5 14 4826.5 15 5016.5 16 5265.0 17 5549.5 18 5822.0 19 6203.0 20 6872.0 21 7762.0 22 9068.0 23 10726.5 24 12755.5 25 15551.5 26 19417.5 27 24397.0 28 29583.0 29 34864.0 30 40911.0 31 48410.0 32 55641.5 33 62298.5 34 69913.0 35 76752.0 36 84256.5 37 91965.5 38 97329.0 39 101547.5 40 106189.5 41 115092.0 42 124538.0 43 131910.5 44 139041.0 45 144429.0 46 147637.5 47 155511.0 48 176640.0 49 179820.5 50 164832.0 51 156565.0 52 149108.5 53 145281.0 54 141066.5 55 131224.0 56 119618.5 57 108423.5 58 97794.0 59 86376.5 60 73440.5 61 59796.5 62 48172.0 63 38611.5 64 29957.0 65 23491.0 66 18139.0 67 14382.0 68 10749.5 69 7285.5 70 5463.0 71 4217.0 72 3319.5 73 2646.0 74 2017.5 75 1705.0 76 1270.0 77 765.0 78 479.5 79 324.5 80 220.5 81 165.5 82 133.0 83 105.0 84 86.0 85 61.5 86 37.0 87 35.0 88 33.0 89 23.0 90 14.5 91 10.0 92 10.0 93 6.5 94 6.5 95 6.5 96 4.5 97 3.5 98 3.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2771354825829146 2 0.16648884465439356 3 0.19440994801732986 4 0.16396853266942057 5 0.23579742424115138 6 0.19638666329966165 7 0.24086275715212652 8 0.24721295499661733 9 0.14981030945971924 10-11 0.20581312430228127 12-13 0.21182975144287858 14-15 0.17322203108483616 16-17 0.2852153062994457 18-19 0.2317945757944295 20-21 0.19822747940633312 22-23 0.2433583601960704 24-25 0.23262232531890598 26-27 0.3419470349023676 28-29 0.31337114460215887 30-31 0.28889693851278864 32-33 0.1915437108579488 34-35 0.3586996969201293 36-37 0.3963684775190642 38-39 0.37703373116375655 40-41 0.3382654026890246 42-43 0.37232667789770396 44-45 0.38155546737209045 46-47 0.3864972555779199 48-49 0.4063755986358688 50-51 0.39925942361947436 52-53 0.39869111797580403 54-55 0.3933786956545374 56-57 0.3710665219052175 58-59 0.3449738801784381 60-61 0.3111226309685065 62-63 0.3810612885515075 64-65 0.41356590047535063 66-67 0.419088348795365 68-69 0.42420309958839847 70-71 0.4243142898230296 72-73 0.4158885409320905 74-75 0.41034138367104694 76-77 0.39473768741114046 78-79 0.36850914650870076 80-81 0.37983819597056473 82-83 0.3899565073220005 84-85 0.39004298861560255 86-87 0.3860030767573369 88-89 0.3944658890598199 90-91 0.4151719816422452 92-93 0.414134206119021 94-95 0.4228935257138537 96-97 0.423202387476718 98-99 0.4206326576096867 100-101 0.3937987476520329 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4047118.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.26577650552317 #Duplication Level Percentage of deduplicated Percentage of total 1 72.56125510114295 35.74786576773161 2 14.42527800478156 14.213450444272151 3 5.277284105079521 7.799684978309926 4 2.591926188668632 5.107730253190452 5 1.404185752853936 3.45891507361709 6 0.8613787752739531 2.5461896537548703 7 0.5862076370342988 2.021598210237383 8 0.3948228221213105 1.5561002331126732 9 0.27476097236874236 1.2182681391442818 >10 1.4403928519619933 12.898487240712313 >50 0.10947808416686541 3.704016140041904 >100 0.06724109609099746 6.026103353642343 >500 0.00392618665627303 1.3538376369312866 >1k 0.001661078901848171 1.3831739667247573 >5k 1.0067144859685847E-4 0.35843441768183293 >10k+ 1.0067144859685847E-4 0.6061444908950493 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13716 0.3389078351557825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10727 0.26505281041966156 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7526 0.18595949018536154 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6928 0.17118354344993153 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4361 0.10775569182811078 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.470894102914716E-5 0.0 0.0 0.0 6 0.0 2.470894102914716E-5 0.0 0.0 0.0 7 0.0 2.470894102914716E-5 0.0 0.0 0.0 8 0.0 2.470894102914716E-5 0.0 0.0 0.0 9 0.0 2.470894102914716E-5 0.0 2.470894102914716E-5 0.0 10-11 0.0 2.470894102914716E-5 0.0 4.941788205829432E-5 0.0 12-13 0.0 2.470894102914716E-5 0.0 7.412682308744148E-5 0.0 14-15 0.0 2.470894102914716E-5 0.0 7.412682308744148E-5 0.0 16-17 0.0 2.470894102914716E-5 0.0 9.883576411658865E-5 0.0 18-19 0.0 2.470894102914716E-5 0.0 1.6060811668945656E-4 0.0 20-21 0.0 2.470894102914716E-5 0.0 2.3473493977689802E-4 0.0 22-23 0.0 2.470894102914716E-5 0.0 5.065332910975167E-4 0.0 24-25 0.0 2.470894102914716E-5 0.0 8.153950539618563E-4 0.0 26-27 0.0 2.470894102914716E-5 0.0 0.0014454730502051088 0.0 28-29 0.0 2.470894102914716E-5 0.0 0.0036569232723137795 0.0 30-31 0.0 2.470894102914716E-5 0.0 0.0100071211168046 0.0 32-33 0.0 3.706341154372074E-5 0.0 0.022954606216077714 0.0 34-35 0.0 4.941788205829432E-5 0.0 0.044006623972911094 0.0 36-37 0.0 4.941788205829432E-5 0.0 0.07938982752664983 0.0 38-39 0.0 4.941788205829432E-5 0.0 0.1308338427493342 0.0 40-41 0.0 4.941788205829432E-5 0.0 0.18567533736352634 0.0 42-43 0.0 4.941788205829432E-5 0.0 0.24160402538300096 0.0 44-45 0.0 4.941788205829432E-5 0.0 0.31732457516682244 0.0 46-47 0.0 4.941788205829432E-5 0.0 0.4093406715593665 0.0 48-49 0.0 4.941788205829432E-5 0.0 0.5068668618014103 0.0 50-51 0.0 4.941788205829432E-5 0.0 0.6110644661213238 0.0 52-53 0.0 4.941788205829432E-5 0.0 0.7167569613735008 0.0 54-55 0.0 4.941788205829432E-5 0.0 0.8351004344326012 0.0 56-57 0.0 4.941788205829432E-5 0.0 0.9819703799098519 0.0 58-59 0.0 4.941788205829432E-5 0.0 1.14485171917399 0.0 60-61 0.0 4.941788205829432E-5 0.0 1.3266106893843965 0.0 62-63 0.0 4.941788205829432E-5 0.0 1.5042432664429355 0.0 64-65 0.0 4.941788205829432E-5 0.0 1.6881024966408193 0.0 66-67 0.0 4.941788205829432E-5 0.0 1.9111748162519602 0.0 68-69 0.0 4.941788205829432E-5 0.0 2.163589003335213 0.0 70-71 0.0 4.941788205829432E-5 0.0 2.4353626457148025 0.0 72-73 0.0 4.941788205829432E-5 0.0 2.7110155918359684 0.0 74-75 0.0 4.941788205829432E-5 0.0 2.993241116270887 0.0 76-77 0.0 4.941788205829432E-5 0.0 3.2992366419758454 0.0 78-79 0.0 4.941788205829432E-5 0.0 3.6482998518946075 0.0 80-81 0.0 4.941788205829432E-5 0.0 4.032684493014536 0.0 82-83 0.0 4.941788205829432E-5 0.0 4.425025413145848 0.0 84-85 0.0 4.941788205829432E-5 0.0 4.817564004805394 0.0 86-87 0.0 4.941788205829432E-5 0.0 5.221999951570476 0.0 88-89 0.0 4.941788205829432E-5 0.0 5.6752113479270925 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16470 0.0 25.158186 1 GTATCAA 25835 0.0 23.984217 1 TACATGG 16915 0.0 23.978588 2 ACATGGG 16875 0.0 23.135855 3 GAGTACT 12405 0.0 22.784555 12-13 GGTATCA 19320 0.0 22.209414 1 ATCAACG 29720 0.0 20.638552 3 TCAACGC 29760 0.0 20.610813 4 TATCAAC 29860 0.0 20.510014 2 CATGGGG 8875 0.0 20.471985 4 CAACGCA 29975 0.0 20.431328 5 GTACTTT 13720 0.0 20.410618 14-15 AACGCAG 30505 0.0 20.1072 6 AGTACTT 13485 0.0 19.49909 12-13 ACGCAGA 33145 0.0 18.320059 7 AGAGTAC 24225 0.0 18.033857 10-11 CGCAGAG 33490 0.0 18.018011 8 CATGGGA 8635 0.0 17.854616 4 GTATAGG 935 0.0 17.787416 1 GTGTAGC 2120 0.0 17.70711 1 >>END_MODULE