FastQCFastQC Report
Thu 26 May 2016
SRR938724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4047118
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134050.3312233544957177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116610.28813096134088506No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82730.20441706913413446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77560.1916425466220654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG160100.022.5835841
GAGTACT126500.021.75549912-13
TACATGG163750.021.5635262
ACATGGG161200.021.0213853
GTATCAA268350.020.9194871
GGTATCA208650.020.7032281
GTACTTT139350.019.76636514-15
AGTACTT138800.018.57982412-13
TACCTGG35550.018.4233112
TCAACGC308500.018.0455864
ATCAACG309900.017.9946943
TATCAAC312500.017.9209142
CGCGGAA5200.017.8947182-83
CAACGCA311750.017.8270135
TGCACCG9950.017.6484585
CATGGGG86000.017.6043664
AACGCAG318150.017.4794316
AGAGTAC243400.017.47677410-11
ACTTTTT163650.016.85988216-17
GCACCGT9600.016.8050296
Produced by FastQC (version 0.11.5)