##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938724_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4047118 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07544603344899 33.0 31.0 34.0 30.0 34.0 2 32.2776921750243 34.0 31.0 34.0 30.0 34.0 3 32.358281868727325 34.0 31.0 34.0 30.0 34.0 4 35.96903179002935 37.0 35.0 37.0 35.0 37.0 5 35.8724707310239 37.0 35.0 37.0 35.0 37.0 6 35.89911882974502 37.0 35.0 37.0 35.0 37.0 7 35.82133335375939 37.0 35.0 37.0 33.0 37.0 8 35.804432932274274 37.0 35.0 37.0 33.0 37.0 9 37.45348541851263 39.0 37.0 39.0 34.0 39.0 10-11 37.38118149754962 39.0 37.0 39.0 34.0 39.0 12-13 37.29742312924901 39.0 37.0 39.0 34.0 39.0 14-15 38.52084087985574 40.0 38.0 41.0 34.0 41.0 16-17 38.44488522449802 40.0 38.0 41.0 33.0 41.0 18-19 38.589572258580056 40.0 38.0 41.0 34.0 41.0 20-21 38.59214680669059 40.0 38.0 41.0 34.0 41.0 22-23 38.52077935459258 40.0 38.0 41.0 34.0 41.0 24-25 38.44514417420001 40.0 38.0 41.0 34.0 41.0 26-27 38.27264080266501 40.0 38.0 41.0 33.5 41.0 28-29 38.10505969927242 40.0 38.0 41.0 33.0 41.0 30-31 37.89895587427893 40.0 37.0 41.0 33.0 41.0 32-33 37.767399171459786 40.0 37.0 41.0 33.0 41.0 34-35 37.755144277977564 40.0 37.0 41.0 33.0 41.0 36-37 37.65233309233879 40.0 37.0 41.0 32.5 41.0 38-39 37.53868740669286 40.0 37.0 41.0 32.0 41.0 40-41 37.37432859629988 40.0 36.5 41.0 31.5 41.0 42-43 37.186215104180306 40.0 36.0 41.0 31.0 41.0 44-45 36.95718286444823 40.0 36.0 41.0 31.0 41.0 46-47 36.668947384286795 39.5 36.0 41.0 30.0 41.0 48-49 36.38342259850096 39.0 35.0 41.0 30.0 41.0 50-51 36.15709561717746 39.0 35.0 41.0 29.5 41.0 52-53 35.93334960828916 39.0 35.0 40.5 28.5 41.0 54-55 35.65690671732329 38.5 35.0 40.0 28.0 41.0 56-57 35.386972161424495 38.0 34.5 40.0 28.0 41.0 58-59 35.07801798712071 38.0 34.0 40.0 27.0 41.0 60-61 34.7081232867438 37.5 34.0 40.0 26.0 41.0 62-63 34.34292167908126 37.0 33.5 40.0 26.0 41.0 64-65 33.937871838676315 36.5 33.0 39.0 26.0 41.0 66-67 33.52396483127006 36.0 33.0 39.0 25.0 40.0 68-69 33.17677320008954 35.5 32.0 39.0 24.5 40.0 70-71 32.7318804393645 35.0 32.0 38.0 23.0 40.0 72-73 32.23719805056339 35.0 31.5 37.5 22.0 39.5 74-75 31.73470640095001 35.0 31.0 37.0 20.5 39.0 76-77 29.8012499264909 33.0 28.5 35.0 18.5 37.0 78-79 30.75847850248004 34.0 30.5 36.0 18.5 38.0 80-81 30.806215805914235 34.0 31.0 35.5 18.5 37.0 82-83 30.480362569117084 34.0 30.5 35.0 18.0 37.0 84-85 30.07700183192089 34.0 30.0 35.0 13.0 36.0 86-87 29.477446172807415 34.0 29.5 35.0 7.0 36.0 88-89 28.868899547776962 34.0 29.0 35.0 2.0 35.5 90-91 28.338954658599032 33.5 28.5 35.0 2.0 35.0 92-93 28.02975500096612 33.0 27.5 35.0 2.0 35.0 94-95 27.6596008567084 33.0 27.0 35.0 2.0 35.0 96-97 27.009034947832014 33.0 26.5 35.0 2.0 35.0 98-99 26.122791082444348 33.0 24.5 35.0 2.0 35.0 100-101 22.344286971617827 28.5 9.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14785833494807576 1101 2 0.18918689016161494 1101 3 0.04603876738308088 1101 4 0.08846807503815768 1101 5 -0.0559424811302307 1101 6 0.05797447270091283 1101 7 0.15868143922056532 1101 8 0.1842503052047917 1101 9 0.16316277806839707 1101 10-11 0.2753236901050684 1101 12-13 0.22967302091468866 1101 14-15 0.3222746864045192 1101 16-17 0.38449074745687284 1101 18-19 0.3219093195398628 1101 20-21 0.30026632433838074 1101 22-23 0.2103343443420016 1101 24-25 0.26832847018776107 1101 26-27 0.22669836016734024 1101 28-29 0.23741621165289217 1101 30-31 0.293960727554861 1101 32-33 0.32414228219585084 1101 34-35 0.41674947888225944 1101 36-37 0.43322548189591714 1101 38-39 0.48936330453427956 1101 40-41 0.5791592784728508 1101 42-43 0.5386990283306474 1101 44-45 0.501156488521751 1101 46-47 0.4648591149103183 1101 48-49 0.39718719185504625 1101 50-51 0.3975382220591541 1101 52-53 0.41344723516402837 1101 54-55 0.43446045749973194 1101 56-57 0.3225419318552696 1101 58-59 0.3373452699169164 1101 60-61 0.4418841943971259 1101 62-63 0.30252599602313524 1101 64-65 0.2678718131705793 1101 66-67 0.36793066189201085 1101 68-69 0.3797556564310298 1101 70-71 0.4814425568873375 1101 72-73 0.3302169197209608 1101 74-75 0.3035035763698488 1101 76-77 0.2521155279826033 1101 78-79 0.0811267711135848 1101 80-81 0.38657114908259516 1101 82-83 0.5031164415477178 1101 84-85 0.49240459679655757 1101 86-87 0.47053278995694825 1101 88-89 0.5118326444074448 1101 90-91 0.4251270506451448 1101 92-93 0.4862470322445205 1101 94-95 0.5816483173585958 1101 96-97 0.5430991279329866 1101 98-99 0.4259019183331141 1101 100-101 1.0713313915105438 1102 1 0.28940184748694975 1102 2 0.20881643900765567 1102 3 0.21627939496394788 1102 4 0.1696625222506114 1102 5 -0.12426396824119479 1102 6 9.391000115286374E-4 1102 7 0.14690442565327544 1102 8 0.19634199771164162 1102 9 0.27574145165964836 1102 10-11 0.2697238858230264 1102 12-13 0.26986295125033166 1102 14-15 0.39322528520460054 1102 16-17 0.37055204382921403 1102 18-19 0.3594746115634635 1102 20-21 0.3224255833496912 1102 22-23 0.3364237273522406 1102 24-25 0.3485210043373641 1102 26-27 0.384780904155285 1102 28-29 0.3976941348582699 1102 30-31 0.4848879784116633 1102 32-33 0.47772701520774064 1102 34-35 0.4946213484052677 1102 36-37 0.4196715195481815 1102 38-39 0.49188428390151984 1102 40-41 0.5142071081291206 1102 42-43 0.6269474358855334 1102 44-45 0.5874204134504666 1102 46-47 0.49808430563814454 1102 48-49 0.4588186355619186 1102 50-51 0.505932406175333 1102 52-53 0.3580051251368417 1102 54-55 0.3877656198694339 1102 56-57 0.3770464220460781 1102 58-59 0.3804565314431869 1102 60-61 0.3679399499520599 1102 62-63 0.37397915560021033 1102 64-65 0.3922865797574744 1102 66-67 0.4785445048653685 1102 68-69 0.41553940289358593 1102 70-71 0.40592936027069015 1102 72-73 0.35287787568472595 1102 74-75 0.323078395761101 1102 76-77 0.25864949680285676 1102 78-79 0.22407409108252452 1102 80-81 0.412322684145078 1102 82-83 0.3965075667994995 1102 84-85 0.5437502040482407 1102 86-87 0.592550721103347 1102 88-89 0.4390224473289628 1102 90-91 0.56021230241452 1102 92-93 0.6198127240954783 1102 94-95 0.7540696391397148 1102 96-97 0.7934574762881255 1102 98-99 0.9466828527382916 1102 100-101 1.5078059816489393 1103 1 0.24292673507079598 1103 2 0.2255750695476877 1103 3 0.178001987004059 1103 4 0.12777881214421427 1103 5 0.10734294930531263 1103 6 0.13182034325031822 1103 7 0.122014450200858 1103 8 0.1939122454343618 1103 9 0.18992287974246125 1103 10-11 0.24006307694014595 1103 12-13 0.20696560758775462 1103 14-15 0.23160334654564707 1103 16-17 0.2212174284327233 1103 18-19 0.1602229970617941 1103 20-21 0.28861248948550866 1103 22-23 0.2889741710499507 1103 24-25 0.32757397251317855 1103 26-27 0.3356798368140943 1103 28-29 0.27425269863915247 1103 30-31 0.39242285637640606 1103 32-33 0.5036271270199322 1103 34-35 0.4549101262495512 1103 36-37 0.4227888438877798 1103 38-39 0.4017338445147587 1103 40-41 0.43998535088116597 1103 42-43 0.49771391949634136 1103 44-45 0.5149086253980073 1103 46-47 0.5333591258785617 1103 48-49 0.5362037328915719 1103 50-51 0.4488465480056618 1103 52-53 0.39985959102818924 1103 54-55 0.3889832508979012 1103 56-57 0.41455309659919237 1103 58-59 0.4187512255798751 1103 60-61 0.44703559167397344 1103 62-63 0.45022360744889056 1103 64-65 0.520148403202704 1103 66-67 0.5358721575957048 1103 68-69 0.46105387614623794 1103 70-71 0.5333836113896098 1103 72-73 0.5713665830886114 1103 74-75 0.6696888247325674 1103 76-77 0.6716403281056955 1103 78-79 0.536099633210025 1103 80-81 0.6513371068712352 1103 82-83 0.6842476350129942 1103 84-85 0.8056264886669915 1103 86-87 0.7820887757285142 1103 88-89 0.6560058042221506 1103 90-91 0.539942489110679 1103 92-93 0.7248824963726541 1103 94-95 0.9157876053868073 1103 96-97 0.8573848157360224 1103 98-99 0.9685269493454349 1103 100-101 1.488439408925025 1104 1 0.09401378734306576 1104 2 0.03707572169372497 1104 3 0.05562302459946977 1104 4 0.029666676675702774 1104 5 -0.0965767410103453 1104 6 -0.026259964270821 1104 7 0.014255324452705054 1104 8 -0.050287091928062466 1104 9 0.031182554523908834 1104 10-11 0.06842767999394539 1104 12-13 0.001447850023112096 1104 14-15 -0.002140442804396514 1104 16-17 0.08489985849001158 1104 18-19 0.14420639134340973 1104 20-21 0.14102669770041842 1104 22-23 0.025437558995776044 1104 24-25 0.057649147471344975 1104 26-27 0.07055780624776986 1104 28-29 0.06788552281425808 1104 30-31 -0.11391929838428894 1104 32-33 -0.11687567619251382 1104 34-35 -0.11505469403802948 1104 36-37 0.06439890370157997 1104 38-39 0.007486568139313476 1104 40-41 -0.05603645614201014 1104 42-43 0.006211166638472321 1104 44-45 -0.017566510670555147 1104 46-47 -0.07621138310781106 1104 48-49 0.06345962759350954 1104 50-51 -0.08058964641787014 1104 52-53 -0.035524261780651045 1104 54-55 -0.11169707688525676 1104 56-57 -0.17538780465665127 1104 58-59 0.07724125002288673 1104 60-61 0.22112323317909244 1104 62-63 0.06726489304607242 1104 64-65 -0.13909672979772125 1104 66-67 -0.17948117960975907 1104 68-69 -0.11235479558737183 1104 70-71 -0.11228452974120984 1104 72-73 -0.03781115407701208 1104 74-75 -0.01953318361283607 1104 76-77 -0.012797092734540882 1104 78-79 -0.2198500802194694 1104 80-81 -0.08351938074099508 1104 82-83 0.0054678349195178555 1104 84-85 0.0591086168130559 1104 86-87 0.20524247176152244 1104 88-89 0.23679974590384134 1104 90-91 0.25790170645851873 1104 92-93 0.313287642683985 1104 94-95 0.3268760288903074 1104 96-97 0.4213500787594455 1104 98-99 0.7079901910346145 1104 100-101 1.5343667920762343 1105 1 0.1163393231103882 1105 2 0.13182781425541634 1105 3 0.05114564906008212 1105 4 0.08895388579467323 1105 5 -0.018110638104104737 1105 6 0.003661961734657382 1105 7 0.08197979991774673 1105 8 0.11616517064677367 1105 9 0.20417914641780754 1105 10-11 0.12429171092432512 1105 12-13 0.1607825757245962 1105 14-15 0.24052530420159002 1105 16-17 0.17892667638560766 1105 18-19 0.27245943054921185 1105 20-21 0.18734632240019522 1105 22-23 0.19412941659213345 1105 24-25 0.22909673857727597 1105 26-27 0.3106121313162262 1105 28-29 0.28640280174713695 1105 30-31 0.2987557236619338 1105 32-33 0.29061595279979 1105 34-35 0.34291677751622984 1105 36-37 0.3576156979044427 1105 38-39 0.3988681850634137 1105 40-41 0.35614701101389556 1105 42-43 0.37218472803267133 1105 44-45 0.45225522078501257 1105 46-47 0.38360415093983136 1105 48-49 0.4451618918950473 1105 50-51 0.45892746265405293 1105 52-53 0.414491869742065 1105 54-55 0.43164626431366315 1105 56-57 0.4839429701094815 1105 58-59 0.45271587442355354 1105 60-61 0.48822639333034346 1105 62-63 0.4845120838139678 1105 64-65 0.39991509854180407 1105 66-67 0.5449323289133119 1105 68-69 0.4852964933365129 1105 70-71 0.5083378192543364 1105 72-73 0.5783984098556161 1105 74-75 0.5536026977752897 1105 76-77 0.6025813699968978 1105 78-79 0.4211699529543367 1105 80-81 0.5555611821672031 1105 82-83 0.6491554225790672 1105 84-85 0.598402863864866 1105 86-87 0.6121864425814962 1105 88-89 0.5920855296424001 1105 90-91 0.44792328851605134 1105 92-93 0.5649549959354196 1105 94-95 0.8049155888158133 1105 96-97 0.8569375114369677 1105 98-99 1.0596473772124035 1105 100-101 1.8535747706009396 1106 1 0.11064612438844534 1106 2 0.09811719007124964 1106 3 0.0701251201295392 1106 4 0.04664022724715977 1106 5 -0.03788454338002367 1106 6 -0.021023048230176755 1106 7 0.031439226394255115 1106 8 0.023193223557342435 1106 9 0.08868171971360539 1106 10-11 0.07464068193014839 1106 12-13 0.04642937311976425 1106 14-15 0.073458832975966 1106 16-17 0.14090643144965753 1106 18-19 0.02164790776157588 1106 20-21 0.027363196023507896 1106 22-23 0.08202155687976642 1106 24-25 0.08923344040264425 1106 26-27 0.10912951350253763 1106 28-29 0.1796661020372312 1106 30-31 0.16331495598258527 1106 32-33 0.09785766699886977 1106 34-35 0.12475246304584431 1106 36-37 0.06059227284324464 1106 38-39 0.15652554701185295 1106 40-41 0.2287478851076159 1106 42-43 0.2491781067619243 1106 44-45 0.21370562221597567 1106 46-47 0.2605346061580178 1106 48-49 0.19201000119281986 1106 50-51 0.26457300101032644 1106 52-53 0.2252163277724435 1106 54-55 0.3509723236549931 1106 56-57 0.25419967756241135 1106 58-59 0.3232324249845462 1106 60-61 0.2841791803954763 1106 62-63 0.3036876840269258 1106 64-65 0.27373605752455177 1106 66-67 0.24057835746449285 1106 68-69 0.18367692234463107 1106 70-71 0.3103924240099545 1106 72-73 0.3825847369207125 1106 74-75 0.5245432384977988 1106 76-77 0.5067520472199298 1106 78-79 0.34453028226522875 1106 80-81 0.32965028117645545 1106 82-83 0.43628254035470704 1106 84-85 0.47282724026813483 1106 86-87 0.46204574185754055 1106 88-89 0.5623991952414436 1106 90-91 0.6160315092211341 1106 92-93 0.8194555197388169 1106 94-95 0.9674711011144161 1106 96-97 0.9963795661338466 1106 98-99 1.393733574859219 1106 100-101 1.8156057061077817 1107 1 -0.033588362356162804 1107 2 -0.040531654974358844 1107 3 -0.04706421495453128 1107 4 -0.030567064130174515 1107 5 -0.13111995880167626 1107 6 -0.10440130035336637 1107 7 -0.04172066036887401 1107 8 -0.018450057843026002 1107 9 -0.022070901484362082 1107 10-11 -0.05443776743170048 1107 12-13 0.018238722176079136 1107 14-15 0.03006068864664968 1107 16-17 0.006519063701965422 1107 18-19 -0.007688803599428695 1107 20-21 -0.007563926676887434 1107 22-23 -0.03949586212752365 1107 24-25 -0.019865125618999002 1107 26-27 -0.020540174871605643 1107 28-29 0.004231265144909457 1107 30-31 0.013453500007770458 1107 32-33 0.054591080574390105 1107 34-35 0.044768455369528226 1107 36-37 0.004200365520162563 1107 38-39 -0.04646097735118815 1107 40-41 -0.1724398395214095 1107 42-43 -0.12310641070478567 1107 44-45 -0.08451967876236921 1107 46-47 -0.21811550143945624 1107 48-49 -0.08533201849738248 1107 50-51 -0.30268866426752794 1107 52-53 -0.26848070466952123 1107 54-55 -0.19311321002567894 1107 56-57 -0.13142330761522913 1107 58-59 -0.1900496206861675 1107 60-61 -0.09493063636616483 1107 62-63 -0.16918998030823218 1107 64-65 -0.24751636587362214 1107 66-67 -0.18526616348124492 1107 68-69 -0.1738024349322984 1107 70-71 -0.19010577895788572 1107 72-73 -0.17815396814997797 1107 74-75 -0.18612958942198787 1107 76-77 -0.2483963026247764 1107 78-79 -0.1519197866139912 1107 80-81 0.01016346642648358 1107 82-83 -0.08779073254447667 1107 84-85 -0.21347959911562597 1107 86-87 -0.11651508929438137 1107 88-89 -0.06491314045145558 1107 90-91 0.21851141360387416 1107 92-93 0.35370785054985276 1107 94-95 0.4384328570154459 1107 96-97 0.5380645678593368 1107 98-99 0.8445131304970737 1107 100-101 1.628765705097738 1108 1 0.15191184831670768 1108 2 0.06441708129374035 1108 3 0.059463529746587085 1108 4 0.07316520423653117 1108 5 0.004482901464200495 1108 6 0.03612000813888727 1108 7 0.009007351795197849 1108 8 0.062241559819298686 1108 9 0.069073876576347 1108 10-11 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-1.9392989179751865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 17.0 4 37.0 5 104.0 6 269.0 7 611.0 8 1410.0 9 2607.0 10 4213.0 11 6277.0 12 8625.0 13 11847.0 14 14722.0 15 19335.0 16 24191.0 17 32503.0 18 31733.0 19 23534.0 20 22871.0 21 24540.0 22 26376.0 23 29425.0 24 34312.0 25 40856.0 26 50273.0 27 60413.0 28 73521.0 29 88819.0 30 108318.0 31 130781.0 32 160077.0 33 202992.0 34 268462.0 35 373422.0 36 561009.0 37 858541.0 38 690209.0 39 59853.0 40 5.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.649367194570026 19.449522925482462 14.773930130117535 23.127179749829978 2 15.914485320171046 20.708266969235886 39.28489359588724 24.09235411470582 3 19.76510198121231 24.280660954288955 29.74388194265648 26.21035512184226 4 13.445518514656602 17.004470835789814 36.20116833756762 33.34884231198596 5 14.000465657281183 37.24946822630548 33.918585113697986 14.831481002715346 6 32.30939501195466 35.912621733470786 17.128577627233902 14.649405627340647 7 28.948377586223085 32.43221472662769 20.248482994565514 18.37092469258371 8 26.271855676063804 35.344114009030626 19.229535684405544 19.154494630500025 9 26.209638562552414 17.18160923402777 20.095238142302748 36.51351406111707 10-11 25.321055625262222 26.471491565108803 26.876903515044532 21.33054929458444 12-13 26.26991602419302 23.721497618799354 27.486497799174625 22.522088557833005 14-15 22.904694155198836 25.23925667598523 25.965007691893344 25.891041476922588 16-17 22.445398456783185 28.633573354689062 26.442790599755227 22.478237588772526 18-19 22.30647833841267 27.209164645063478 28.62978050059326 21.854576515930596 20-21 23.079855838154458 26.172525733126633 28.66289048157232 22.084727947146586 22-23 22.72517134415157 26.197358218860927 28.56692095461511 22.510549482372394 24-25 22.81334501082936 26.341673501807033 28.68811881493924 22.156862672424367 26-27 22.631809653756658 26.431894999514466 28.73034965815985 22.20594568856903 28-29 22.201045287041296 26.796698292463923 28.74733328753943 22.254923132955355 30-31 22.50389042276504 26.327327248674244 28.674985508206085 22.493796820354632 32-33 22.36810243733936 26.58383076549782 28.59085156400184 22.45721523316098 34-35 22.931499679875024 26.417180048615506 28.382705393345997 22.268614878163472 36-37 22.84994187529285 26.26203764662155 28.48690167632446 22.40111880176114 38-39 22.578531880428162 26.508221089036365 28.654180479686058 22.259066550849415 40-41 22.654337217566127 26.40595454963294 28.586585212766273 22.353123020034655 42-43 22.548608489027444 26.57532178320579 28.247843246951188 22.62822648081557 44-45 22.635120317600066 26.58749068594789 28.04900152002365 22.72838747642839 46-47 22.77313226053822 26.807165096217865 27.75456151658543 22.66514112665849 48-49 22.96630761013941 26.819270323766137 27.602027308096545 22.61239475799791 50-51 22.58914464162324 26.96680463732635 27.744580616841645 22.699470104208764 52-53 22.887929138710557 27.110365944358428 27.340702692632142 22.66100222429887 54-55 22.569591496961543 26.744797161832196 27.608164624802146 23.07744671640412 56-57 22.770991246008222 27.11818778434165 27.370740990761703 22.74007997888842 58-59 22.999220800945466 26.78228348193134 27.384392965390443 22.83410275173275 60-61 22.83513441254771 26.693271440848548 27.931207620030353 22.540386526573393 62-63 22.675793411546103 26.755030787629604 27.769960069976378 22.79921573084792 64-65 22.509151101023114 26.88077549743591 27.610046715462655 23.000026686078318 66-67 22.48782954017271 26.991054115890005 27.389026484294476 23.1320898596428 68-69 22.344262789262253 26.842687095420775 27.652636229443956 23.160413885873016 70-71 22.493077783329056 26.81687493853645 27.47594952847881 23.21409774965568 72-73 22.5715929211849 26.670991554978134 27.555855796643435 23.201559727193523 74-75 22.369120220977905 27.10783260563146 27.250972084589424 23.27207508880121 76-77 22.44897378938299 26.81668885600664 27.401449044251354 23.332888310359024 78-79 22.45594878115981 26.843805623467194 27.40390807302463 23.296337522348363 80-81 22.37413976526524 27.041790763386476 27.32518037394135 23.258889097406932 82-83 22.423143390719535 26.881392568769304 27.39038892713318 23.305075113377985 84-85 22.328481650399123 26.826237831464265 27.215317171379734 23.629963346756877 86-87 22.398699130040743 26.73971445271175 27.372080419844202 23.489505997403306 88-89 22.346682973236124 26.72504398694631 27.209851984902038 23.718421054915524 90-91 22.296632047555036 26.720708433685925 27.37684011447699 23.605819404282048 92-93 22.381200354080917 26.766083594477273 27.282255987161168 23.570460064280635 94-95 22.21045524488786 26.66419164798563 27.236402788905412 23.8889503182211 96-97 22.14992514417067 26.58773056250221 27.33143354287365 23.93091075045347 98-99 22.129401712527287 26.558578227766027 27.063851362871063 24.248168696835627 100-101 22.208779021160623 26.005366600540615 27.702607455248163 24.0832469230506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1644.0 1 1093.5 2 694.0 3 885.5 4 776.5 5 759.5 6 1372.5 7 2932.5 8 4618.5 9 5226.5 10 4538.0 11 3696.5 12 3621.5 13 3885.5 14 4245.5 15 4575.5 16 4805.0 17 5033.0 18 5321.0 19 5786.5 20 6595.0 21 7509.0 22 8653.5 23 10373.5 24 12647.0 25 15621.5 26 19569.5 27 24403.0 28 29359.0 29 34876.5 30 41443.5 31 48986.5 32 56060.5 33 62906.0 34 70523.5 35 77165.0 36 84538.5 37 91973.0 38 97681.5 39 102120.0 40 106701.5 41 115779.0 42 125380.0 43 133657.0 44 141155.5 45 145625.5 46 148086.5 47 156987.5 48 179615.0 49 182316.5 50 166423.5 51 157684.5 52 149928.0 53 146460.0 54 142313.5 55 131824.5 56 120020.5 57 108624.0 58 97885.0 59 86464.5 60 73068.0 61 59313.5 62 47494.0 63 37549.0 64 29077.5 65 22765.0 66 17226.0 67 13562.5 68 10036.0 69 6580.5 70 4800.0 71 3660.0 72 2897.5 73 2338.0 74 1862.5 75 1575.0 76 1099.5 77 589.0 78 337.5 79 234.0 80 158.5 81 91.5 82 59.0 83 40.5 84 28.5 85 17.0 86 13.5 87 9.0 88 4.0 89 1.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23186870261751696 2 0.0 3 0.0 4 0.0 5 0.030243743819676122 6 0.00400284844672184 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002890946100410218 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.3240646801007534E-4 26-27 0.0012601559924865053 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006449033608607409 36-37 0.015961975904829066 38-39 0.012675686747952494 40-41 0.008153950539618563 42-43 0.007264428662569265 44-45 0.003496315155624323 46-47 6.547869372723997E-4 48-49 0.0018902339887297576 50-51 3.706341154372074E-5 52-53 0.0 54-55 0.0 56-57 0.001235447051457358 58-59 3.335707038934867E-4 60-61 0.002470894102914716 62-63 9.389397591075922E-4 64-65 0.0015813722258654183 66-67 0.005658347495674699 68-69 2.965072923497659E-4 70-71 9.883576411658865E-5 72-73 0.0 74-75 4.076975269809282E-4 76-77 0.05888140647245768 78-79 0.14119924351106145 80-81 9.760031706513129E-4 82-83 8.895218770492979E-4 84-85 0.0 86-87 0.10587781230989557 88-89 0.3554998890568548 90-91 0.3932427964788771 92-93 5.065332910975167E-4 94-95 2.5944388080604517E-4 96-97 1.235447051457358E-4 98-99 0.0 100-101 0.0012230925809427844 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4047118.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.170151357315675 #Duplication Level Percentage of deduplicated Percentage of total 1 69.59374385569025 32.131536873394765 2 15.584771792641694 14.391025450709819 3 6.027054662350291 8.348100779985842 4 2.923282809272177 5.398736390573416 5 1.629439814089964 3.761574142208498 6 1.0004851334843983 2.7715530026231305 7 0.641732626344967 2.0740224742492384 8 0.4345311873584074 1.6049896551849412 9 0.31307175726384506 1.300911337671527 >10 1.6409678458596588 13.686994423729487 >50 0.12650946938082627 4.0116383231435995 >100 0.07815015315406049 6.650122824487995 >500 0.00427958506141819 1.4318293328250504 >1k 0.0017653287996598958 1.4219745543027733 >5k 1.0698962422181135E-4 0.3958944319545462 >10k+ 1.0698962422181135E-4 0.6190960029554342 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13405 0.3312233544957177 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11661 0.28813096134088506 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8273 0.20441706913413446 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7756 0.1916425466220654 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.470894102914716E-5 0.0 12-13 0.0 0.0 0.0 7.412682308744148E-5 0.0 14-15 0.0 0.0 0.0 7.412682308744148E-5 0.0 16-17 0.0 0.0 0.0 8.648129360201506E-5 0.0 18-19 0.0 0.0 0.0 1.4825364617488296E-4 0.0 20-21 0.0 0.0 0.0 2.3473493977689802E-4 0.0 22-23 0.0 0.0 0.0 5.188877616120903E-4 0.0 24-25 0.0 0.0 0.0 8.153950539618563E-4 0.0 26-27 0.0 0.0 0.0 0.0014701819912342562 0.0 28-29 0.0 0.0 0.0 0.003743404565915795 0.0 30-31 0.0 0.0 0.0 0.010229501586066926 0.0 32-33 0.0 0.0 0.0 0.023275822449456626 0.0 34-35 0.0 0.0 0.0 0.04468611985121264 0.0 36-37 0.0 0.0 0.0 0.08034112175627199 0.0 38-39 0.0 0.0 0.0 0.13232873368159762 0.0 40-41 0.0 0.0 0.0 0.18780030629203298 0.0 42-43 0.0 0.0 0.0 0.24421081866157596 0.0 44-45 0.0 0.0 0.0 0.32073440902884476 0.0 46-47 0.0 0.0 0.0 0.4137141541215255 0.0 48-49 0.0 0.0 0.0 0.5120680938880458 0.0 50-51 0.0 0.0 0.0 0.6170440298503774 0.0 52-53 0.0 0.0 0.0 0.7236260469796038 0.0 54-55 0.0 0.0 0.0 0.8429084597978117 0.0 56-57 0.0 0.0 0.0 0.9912485872662966 0.0 58-59 0.0 0.0 0.0 1.1552171199357173 0.0 60-61 0.0 0.0 0.0 1.3376555860244252 0.0 62-63 0.0 0.0 0.0 1.516770699544713 0.0 64-65 0.0 0.0 0.0 1.7034467490199199 0.0 66-67 0.0 0.0 0.0 1.928557556265965 0.0 68-69 0.0 0.0 0.0 2.1820836456955295 0.0 70-71 0.0 0.0 0.0 2.455599268417674 0.0 72-73 0.0 0.0 0.0 2.734069033816162 0.0 74-75 0.0 0.0 0.0 3.019778518936191 0.0 76-77 0.0 0.0 0.0 3.3287885354467055 0.0 78-79 0.0 0.0 0.0 3.680248512645295 0.0 80-81 0.0 0.0 0.0 4.066881667398875 0.0 82-83 0.0 0.0 0.0 4.462125888101113 0.0 84-85 0.0 0.0 0.0 4.857506007979012 0.0 86-87 0.0 0.0 0.0 5.2643634309649485 0.0 88-89 0.0 0.0 0.0 5.720255747423228 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16010 0.0 22.583584 1 GAGTACT 12650 0.0 21.755499 12-13 TACATGG 16375 0.0 21.563526 2 ACATGGG 16120 0.0 21.021385 3 GTATCAA 26835 0.0 20.919487 1 GGTATCA 20865 0.0 20.703228 1 GTACTTT 13935 0.0 19.766365 14-15 AGTACTT 13880 0.0 18.579824 12-13 TACCTGG 3555 0.0 18.423311 2 TCAACGC 30850 0.0 18.045586 4 ATCAACG 30990 0.0 17.994694 3 TATCAAC 31250 0.0 17.920914 2 CGCGGAA 520 0.0 17.89471 82-83 CAACGCA 31175 0.0 17.827013 5 TGCACCG 995 0.0 17.648458 5 CATGGGG 8600 0.0 17.604366 4 AACGCAG 31815 0.0 17.479431 6 AGAGTAC 24340 0.0 17.476774 10-11 ACTTTTT 16365 0.0 16.859882 16-17 GCACCGT 960 0.0 16.805029 6 >>END_MODULE