##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938723_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1207461 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.687392801920726 33.0 31.0 34.0 30.0 34.0 2 31.75718884502274 33.0 31.0 34.0 30.0 34.0 3 31.755272427018348 33.0 31.0 34.0 30.0 34.0 4 35.31073715838441 37.0 35.0 37.0 33.0 37.0 5 35.35159644907786 37.0 35.0 37.0 33.0 37.0 6 35.45135205195033 37.0 35.0 37.0 33.0 37.0 7 35.388805932448335 37.0 35.0 37.0 33.0 37.0 8 35.36996971330751 37.0 35.0 37.0 33.0 37.0 9 36.9422308463793 39.0 37.0 39.0 33.0 39.0 10-11 36.85732748304086 39.0 37.0 39.0 33.0 39.0 12-13 36.7674053240643 39.0 37.0 39.0 32.5 39.0 14-15 37.88416313239102 40.0 38.0 41.0 33.0 41.0 16-17 37.81916765841713 40.0 38.0 41.0 32.5 41.0 18-19 37.827482626768074 40.0 37.5 41.0 32.5 41.0 20-21 37.747138416892966 40.0 37.5 41.0 32.0 41.0 22-23 37.67831300555463 40.0 37.0 41.0 32.0 41.0 24-25 37.696907394938634 40.0 37.0 41.0 32.0 41.0 26-27 37.57163585407727 40.0 37.0 41.0 32.0 41.0 28-29 37.45804295128373 40.0 37.0 41.0 32.0 41.0 30-31 37.27269369362654 40.0 37.0 41.0 31.5 41.0 32-33 37.07528152047975 40.0 36.0 41.0 31.0 41.0 34-35 36.77236987364395 39.0 35.5 41.0 30.5 41.0 36-37 36.50099299273434 39.0 35.0 41.0 30.0 41.0 38-39 36.317689763893 39.0 35.0 41.0 30.0 41.0 40-41 36.15245337116478 39.0 35.0 41.0 30.0 41.0 42-43 35.94577837296608 39.0 35.0 41.0 29.5 41.0 44-45 35.8726824303228 39.0 35.0 41.0 28.5 41.0 46-47 35.64717121298328 39.0 35.0 41.0 27.0 41.0 48-49 35.4209630787247 39.0 35.0 41.0 26.0 41.0 50-51 34.32428790660734 38.0 34.0 40.0 24.0 40.5 52-53 34.42387000491114 38.0 34.5 39.5 24.0 40.5 54-55 34.99704628141198 39.0 35.0 40.5 24.0 41.0 56-57 34.992245298191825 39.0 35.0 41.0 24.0 41.0 58-59 34.850082114453386 39.0 35.0 41.0 23.5 41.0 60-61 34.598746874640256 39.0 34.5 40.5 23.0 41.0 62-63 34.25370301815131 38.0 34.0 40.0 22.0 41.0 64-65 33.85850391855307 38.0 34.0 40.0 21.5 41.0 66-67 33.49032308289874 37.0 33.0 40.0 19.5 41.0 68-69 33.13083445345232 37.0 33.0 39.5 18.5 41.0 70-71 32.70531926082913 36.0 32.5 39.0 15.0 41.0 72-73 32.248634531467275 36.0 32.0 39.0 12.0 40.0 74-75 31.77902102014061 35.0 32.0 38.0 8.0 40.0 76-77 31.28970169636949 35.0 31.0 37.0 6.0 39.0 78-79 30.836166137042937 35.0 31.0 37.0 2.0 39.0 80-81 30.399244778920398 35.0 31.0 36.0 2.0 39.0 82-83 29.916384877027085 34.5 30.0 36.0 2.0 37.5 84-85 29.45730007014719 34.0 30.0 35.0 2.0 37.0 86-87 29.03103909774312 34.0 29.0 35.0 2.0 36.5 88-89 28.599066139610308 34.0 29.0 35.0 2.0 36.0 90-91 28.134161683068854 34.0 29.0 35.0 2.0 36.0 92-93 27.711666877853613 34.0 27.5 35.0 2.0 35.5 94-95 27.250049898091945 34.0 26.5 35.0 2.0 35.0 96-97 26.664433468244525 34.0 25.5 35.0 2.0 35.0 98-99 26.00014700267752 33.5 24.5 35.0 2.0 35.0 100-101 23.804340678498104 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3968183663209075 1101 2 0.21539303547995559 1101 3 0.28968458229529404 1101 4 0.3863495929918841 1101 5 0.031949432940344025 1101 6 0.01592162860195856 1101 7 0.20073086908897864 1101 8 0.37572377532811174 1101 9 0.637290949338059 1101 10-11 0.5786854210376333 1101 12-13 0.5130912147515474 1101 14-15 0.7346604253532192 1101 16-17 0.5646738930979609 1101 18-19 0.6121969580277664 1101 20-21 0.7994014347675957 1101 22-23 0.6780808931656637 1101 24-25 0.7413486144996781 1101 26-27 0.5841501808444605 1101 28-29 0.7142354172791627 1101 30-31 0.6360130125901904 1101 32-33 0.847494432047057 1101 34-35 0.8535235593887194 1101 36-37 0.8344333683894334 1101 38-39 0.9317787783302052 1101 40-41 0.8980664157371265 1101 42-43 0.8703282199204097 1101 44-45 1.0699590287226641 1101 46-47 0.9806439244033314 1101 48-49 1.0837499408667952 1101 50-51 1.1966528647792387 1101 52-53 1.0498797896397747 1101 54-55 1.1397440103930734 1101 56-57 1.2715999669660647 1101 58-59 1.1157563350964352 1101 60-61 0.9210086933624524 1101 62-63 0.9785811864472933 1101 64-65 0.9920233939241925 1101 66-67 1.129404286758529 1101 68-69 1.1597315181111227 1101 70-71 1.0271494019392549 1101 72-73 0.7569231383857513 1101 74-75 0.8470303817432061 1101 76-77 0.9061354076706039 1101 78-79 0.8950264922871938 1101 80-81 0.9593841429234793 1101 82-83 0.5042491554772681 1101 84-85 0.7437897049406743 1101 86-87 0.8138873134299089 1101 88-89 0.6732073120463049 1101 90-91 0.4155026014940688 1101 92-93 0.21629760316316293 1101 94-95 0.09177645238888132 1101 96-97 0.06897244698568983 1101 98-99 0.16920799370101136 1101 100-101 0.3664760956400208 1102 1 0.12865022827917372 1102 2 0.20127092163874138 1102 3 0.10822467983819806 1102 4 0.2104968642599374 1102 5 0.11659568556412125 1102 6 0.211536056309086 1102 7 0.2990601381345357 1102 8 0.2854811527821184 1102 9 0.31723986278416305 1102 10-11 0.32243619273783963 1102 12-13 0.25639594373043195 1102 14-15 0.21494850104157592 1102 16-17 0.28958399002350177 1102 18-19 0.3748235168621932 1102 20-21 0.3737404735378149 1102 22-23 0.382861575966011 1102 24-25 0.2531232163023631 1102 26-27 0.3883821847955602 1102 28-29 0.3654037713969629 1102 30-31 0.41140696554735 1102 32-33 0.3050567082539999 1102 34-35 0.41794239196064353 1102 36-37 0.4581758828974003 1102 38-39 0.4156988610564696 1102 40-41 0.550586016922459 1102 42-43 0.5163389156853171 1102 44-45 0.5200539478484814 1102 46-47 0.6781941620870029 1102 48-49 0.8813391494110618 1102 50-51 0.5485971634580835 1102 52-53 0.5265578517462117 1102 54-55 0.36645216880768317 1102 56-57 0.41614829083320615 1102 58-59 0.3546793502834902 1102 60-61 0.26398815070286474 1102 62-63 0.2982433360954033 1102 64-65 0.3038805676493368 1102 66-67 0.28302433244317626 1102 68-69 0.23463582728509635 1102 70-71 0.20732635279738076 1102 72-73 0.251787859338954 1102 74-75 0.20508037248280075 1102 76-77 0.178695677456993 1102 78-79 0.12089735597406914 1102 80-81 0.08216241801916979 1102 82-83 0.05866675332885052 1102 84-85 -0.19979360781398725 1102 86-87 -0.19308654136698067 1102 88-89 -0.014015261728236084 1102 90-91 -0.06112761519935006 1102 92-93 -0.027504872451110884 1102 94-95 -0.21216886493424525 1102 96-97 -0.2510504725105456 1102 98-99 -0.24113587591252283 1102 100-101 0.103834874501608 1103 1 0.10911279440141897 1103 2 0.1902948518766152 1103 3 0.0909579009010848 1103 4 0.11532909716468254 1103 5 0.14542513249851652 1103 6 0.10868979256857614 1103 7 0.12217478170556717 1103 8 0.011646701199488518 1103 9 0.21158832292667284 1103 10-11 0.2069561623238343 1103 12-13 0.1383734926111444 1103 14-15 0.17562599972729487 1103 16-17 0.27887523084955745 1103 18-19 0.4769060891028829 1103 20-21 0.45399305234246157 1103 22-23 0.4230765976330204 1103 24-25 0.3601937298654079 1103 26-27 0.4064624047030776 1103 28-29 0.4401497520852544 1103 30-31 0.4079493773422769 1103 32-33 0.34599866621495323 1103 34-35 0.3075598259572061 1103 36-37 0.2671208377070542 1103 38-39 0.312267855404329 1103 40-41 0.31971283203559864 1103 42-43 0.2852444570338122 1103 44-45 0.36669749214927094 1103 46-47 0.49055586598409207 1103 48-49 0.5052153311466157 1103 50-51 0.3842464460261681 1103 52-53 0.5232643625411058 1103 54-55 0.5089220295388728 1103 56-57 0.5003815698133849 1103 58-59 0.5229053287145007 1103 60-61 0.5340717149628418 1103 62-63 0.6182872492847977 1103 64-65 0.420567208357177 1103 66-67 0.33338379093133597 1103 68-69 0.23714928934332846 1103 70-71 0.4509082998283134 1103 72-73 0.45871038679026555 1103 74-75 0.47784929435979606 1103 76-77 0.5228925873465897 1103 78-79 0.5948531368625503 1103 80-81 0.6136029696294223 1103 82-83 0.5827945040290601 1103 84-85 0.6649390667472552 1103 86-87 0.6284706467632439 1103 88-89 0.42817325460889677 1103 90-91 0.3631600020831698 1103 92-93 0.4960993950139283 1103 94-95 0.5856292383486235 1103 96-97 0.7304675991702752 1103 98-99 0.46204059065731684 1103 100-101 0.41021995916480947 1104 1 0.027937704300001087 1104 2 0.33081115394685057 1104 3 0.2291662896123654 1104 4 0.2939933212148773 1104 5 0.15616546081490412 1104 6 0.05305141954272585 1104 7 0.016214318566326824 1104 8 0.08783178926539392 1104 9 -0.027234311985978366 1104 10-11 0.07358960222229882 1104 12-13 0.35133163287002134 1104 14-15 0.3329727075831883 1104 16-17 0.22272049588541165 1104 18-19 0.17896525071069647 1104 20-21 0.24255256717177787 1104 22-23 0.3078500569287357 1104 24-25 0.23076499080629986 1104 26-27 0.2663801460012536 1104 28-29 0.2675890758157493 1104 30-31 0.4296650160745088 1104 32-33 0.4288859825697102 1104 34-35 0.28371737471973546 1104 36-37 0.4075278805845599 1104 38-39 0.3485688480166118 1104 40-41 0.36437739134687774 1104 42-43 0.37391053699358423 1104 44-45 0.5338745339491453 1104 46-47 0.49535429025699784 1104 48-49 0.5894990697866547 1104 50-51 0.628392847357631 1104 52-53 0.614786148524793 1104 54-55 0.6634265016822667 1104 56-57 0.5575211342134452 1104 58-59 0.5847894361417261 1104 60-61 0.53260494771785 1104 62-63 0.6768172491650688 1104 64-65 0.7468361116942219 1104 66-67 0.6731978407654964 1104 68-69 0.5506722846616441 1104 70-71 0.5921864228451739 1104 72-73 0.592974967654051 1104 74-75 0.6580386570045285 1104 76-77 0.5783588397263486 1104 78-79 0.6986523633673336 1104 80-81 0.7536567910071064 1104 82-83 0.5733802530382448 1104 84-85 0.5144996352542677 1104 86-87 0.5699630974020344 1104 88-89 0.4947121029870729 1104 90-91 0.5454133157797827 1104 92-93 0.4938019585638642 1104 94-95 0.647993786883653 1104 96-97 0.9866444853132137 1104 98-99 1.2206017219240053 1104 100-101 1.4820466531313095 1105 1 0.14517764593018612 1105 2 0.16231000739692547 1105 3 0.11820259206330164 1105 4 0.16775221814450703 1105 5 0.20429375528466664 1105 6 0.04679006572040123 1105 7 0.17676108886919906 1105 8 0.18735381195814682 1105 9 0.11324485654196792 1105 10-11 0.13813291798513205 1105 12-13 0.1314367580970952 1105 14-15 0.06757022388801914 1105 16-17 -0.07775971496064926 1105 18-19 0.020453978784786386 1105 20-21 0.12671752145867998 1105 22-23 0.13903174786651817 1105 24-25 0.1435006291516956 1105 26-27 0.08076190245617454 1105 28-29 0.10631417185792458 1105 30-31 -0.0662267615245824 1105 32-33 0.034303803231438224 1105 34-35 0.054901001391165494 1105 36-37 -0.018996881915818165 1105 38-39 0.0028639431653658676 1105 40-41 0.03842813734884487 1105 42-43 0.025322878040071828 1105 44-45 0.04562294813658241 1105 46-47 0.25043559107000135 1105 48-49 0.11784639996325552 1105 50-51 -0.08747793497656176 1105 52-53 -0.07574215785717087 1105 54-55 -0.08606871854465936 1105 56-57 -0.13383730409620398 1105 58-59 -0.08004647320638014 1105 60-61 0.06709004256880036 1105 62-63 -0.16062058400446944 1105 64-65 -0.04200438385358751 1105 66-67 -0.046458045353801936 1105 68-69 -0.05534784696823891 1105 70-71 0.07013246054731326 1105 72-73 0.023357051694667064 1105 74-75 0.014914234002059601 1105 76-77 -0.17107384141365145 1105 78-79 -0.2587242708385702 1105 80-81 -0.06298307319373464 1105 82-83 0.04213453398764244 1105 84-85 -0.2199847150837435 1105 86-87 0.00945355274614812 1105 88-89 -0.08108527037127544 1105 90-91 -0.03776739240092297 1105 92-93 -0.04360013798457629 1105 94-95 0.10007544506287402 1105 96-97 -0.06224321235497143 1105 98-99 0.18901873851175566 1105 100-101 0.5497102226241459 1106 1 0.11009914934541598 1106 2 0.18387779339985855 1106 3 0.25625287279140707 1106 4 0.33393252783945826 1106 5 0.14530866784769358 1106 6 0.1984941031984917 1106 7 0.06259951822227094 1106 8 0.2622599379102937 1106 9 0.2538592398341777 1106 10-11 0.22143048977642366 1106 12-13 0.2760170964012829 1106 14-15 0.3181388324064187 1106 16-17 0.23373583272736909 1106 18-19 0.3178380219847341 1106 20-21 0.25876522735575946 1106 22-23 0.10734344546692398 1106 24-25 0.17505303769154779 1106 26-27 0.19360923643321826 1106 28-29 0.1787527514094691 1106 30-31 0.21828849077309798 1106 32-33 0.2402563119036074 1106 34-35 0.16224105789440557 1106 36-37 0.3289772267277513 1106 38-39 0.18659422961532357 1106 40-41 0.24311647550998572 1106 42-43 0.40046681824677677 1106 44-45 0.3712478810836899 1106 46-47 0.20573545159998474 1106 48-49 0.1440757029707953 1106 50-51 0.16974947266925255 1106 52-53 0.13693709687229472 1106 54-55 0.1740106720786656 1106 56-57 0.215706736242808 1106 58-59 0.19346852876051202 1106 60-61 0.0934963652094325 1106 62-63 -0.1545143007015355 1106 64-65 0.0069031010371602974 1106 66-67 -0.07698767379137905 1106 68-69 0.29323420788995946 1106 70-71 0.32144669721719765 1106 72-73 0.14528424119507832 1106 74-75 0.13270917732001308 1106 76-77 0.17642642679791365 1106 78-79 0.2577188861903643 1106 80-81 0.07883647406514527 1106 82-83 0.14231512977493566 1106 84-85 0.18255614247803464 1106 86-87 0.13938417535459635 1106 88-89 0.21794916799370512 1106 90-91 0.17074206652694457 1106 92-93 0.5019707950340546 1106 94-95 0.6359728809603062 1106 96-97 0.6063921272008699 1106 98-99 0.579055572900053 1106 100-101 0.8472927423824004 1107 1 0.11091513573406786 1107 2 -0.034799614194021444 1107 3 0.08679854198705428 1107 4 0.08278927882471976 1107 5 -0.012537822542761035 1107 6 0.04197749700741582 1107 7 -0.09891717477587036 1107 8 0.01699526317636213 1107 9 -0.06912206192453851 1107 10-11 0.05834606122731856 1107 12-13 -0.1274985896349321 1107 14-15 -0.10271916860055086 1107 16-17 -0.1739986765523085 1107 18-19 0.03873074581674274 1107 20-21 0.014863423504998252 1107 22-23 0.17520226246026738 1107 24-25 0.10033646275100949 1107 26-27 0.15212587734712457 1107 28-29 0.06738449848841555 1107 30-31 0.024307692495348476 1107 32-33 0.11875073020948435 1107 34-35 0.07712499685167984 1107 36-37 0.07375057348723857 1107 38-39 0.17255752758924814 1107 40-41 0.2162236475095014 1107 42-43 0.18691276348887698 1107 44-45 0.33821258533203036 1107 46-47 0.46032494100084875 1107 48-49 0.4599058389106574 1107 50-51 0.6691539762196612 1107 52-53 0.6474216538958544 1107 54-55 0.49568465947427853 1107 56-57 0.44001097763589314 1107 58-59 0.4583689610596835 1107 60-61 0.3465736755793998 1107 62-63 0.37109378277213523 1107 64-65 0.41959082131008785 1107 66-67 0.5254290348320296 1107 68-69 0.364799686457296 1107 70-71 0.37921853353380186 1107 72-73 0.22027339031225068 1107 74-75 0.08559551215221717 1107 76-77 0.23595350192074704 1107 78-79 0.260951581202427 1107 80-81 0.09972572561465043 1107 82-83 0.020144605981119668 1107 84-85 0.08080437595430823 1107 86-87 0.274494159902936 1107 88-89 0.18811358057031313 1107 90-91 0.284254453894782 1107 92-93 0.3472215806379708 1107 94-95 0.2803712212092684 1107 96-97 0.24591191053044525 1107 98-99 0.42921095754214633 1107 100-101 0.49954885793995984 1108 1 0.04812896881561102 1108 2 -0.10200517586389068 1108 3 -0.0521208425105435 1108 4 0.06243955533596335 1108 5 0.025959788318075994 1108 6 0.03165327134142615 1108 7 -0.06902524999787119 1108 8 0.03043664894543241 1108 9 0.03625982436747677 1108 10-11 0.02708798634711229 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-3.5618822647495882 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4808.0 3 1084.0 4 1075.0 5 1058.0 6 1239.0 7 1483.0 8 1811.0 9 2219.0 10 2792.0 11 3988.0 12 5302.0 13 7165.0 14 7908.0 15 7958.0 16 10103.0 17 13175.0 18 10895.0 19 7306.0 20 7109.0 21 7464.0 22 8203.0 23 9236.0 24 10540.0 25 12010.0 26 14175.0 27 16459.0 28 19396.0 29 23316.0 30 27838.0 31 33523.0 32 41524.0 33 52398.0 34 69286.0 35 99774.0 36 137414.0 37 222878.0 38 253481.0 39 50059.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.62809294855842 21.842098277538316 15.356985244101123 21.172823529802137 2 13.944024617098671 23.089223719158163 41.51673359219309 21.450018071550076 3 18.069515799874335 29.13929187783177 31.94988071969377 20.84131160260013 4 11.320629577890989 18.90342023122106 41.220190597783954 28.555759593103993 5 11.305487813473547 39.74574676005814 36.53262993972452 12.416135486743789 6 25.92767806248923 40.30788936463539 19.580660020944627 14.183772551930751 7 24.159641411651638 35.59411908480432 24.12318603427634 16.123053469267703 8 22.411420266847404 35.455341629471036 22.753865873675437 19.379372230006123 9 24.882177869988002 18.979057157818684 23.869504119631717 32.269260852561594 10-11 21.712261915414725 28.063490327477126 30.079151628437252 20.145096128670904 12-13 24.892800768934386 26.506568671723844 29.139008919804617 19.461621639537146 14-15 22.484243704006595 27.822430800933134 28.906200479990652 20.78712501506962 16-17 18.72854013243804 30.949271855922262 30.45884345399468 19.863344557645014 18-19 18.293615101393662 30.766276492918994 32.54324979253937 18.39685861314797 20-21 19.528810778562196 29.616850618143246 31.515238142587254 19.3391004607073 22-23 19.19186687329593 29.23351040466739 30.840412043060656 20.734210678976027 24-25 19.432401551160574 29.671644487988118 31.759751635270877 19.13620232558043 26-27 20.12781565368755 28.63795925979651 32.2489172555277 18.98530783098825 28-29 18.87310379237381 28.504798380178908 33.34586213784226 19.276235689605016 30-31 18.97114103303566 30.186683307146122 32.36991752686675 18.472258132951463 32-33 18.759272539858713 30.116629441859903 31.559965911440173 19.564132106841203 34-35 19.306648193233443 29.083197731356076 31.67143038697025 19.938723688440234 36-37 20.216920714392998 28.749712404687898 31.880288789378714 19.15307809154039 38-39 20.401729541762943 28.516266968372843 32.316350575370045 18.76565291449417 40-41 19.03270168924974 28.872609502232528 33.34805464517688 18.746634163340854 42-43 18.72435995031519 29.881270606675503 32.01720997989146 19.377159463117845 44-45 18.912388516827626 30.316142522067825 30.975785093642124 19.795683867462433 46-47 19.125878795215908 29.63684992867297 30.086070864415404 21.15120041169572 48-49 19.533218963338495 29.398275961787718 30.665569291698 20.402935783175792 50-51 20.402756380359854 29.599511064088794 30.65260379591003 19.34512875964132 52-53 19.128151081312843 29.803351139896005 31.676348005613665 19.392149773177483 54-55 18.67360557553448 29.54643033660559 31.63946075209141 20.140503335768525 56-57 19.45686376048432 30.68445231960103 29.963126797646606 19.895557122268045 58-59 18.969625643542123 29.49893124183876 30.83625127876706 20.695191835852054 60-61 19.29893996223475 29.25172924428761 30.852179234434722 20.597151559042914 62-63 20.711681716608513 28.775463852685277 30.787904805166065 19.72494962554014 64-65 20.180950510343358 28.765779696790126 31.293818620042213 19.7594511728243 66-67 18.962826717490543 29.198317152642243 31.212032941196057 20.62682318867116 68-69 18.866060343991634 30.17836341154932 31.00556810808764 19.950008136371412 70-71 19.231696014442843 29.706377965175534 30.225636129747535 20.836289890634085 72-73 19.155902387221104 29.252332520628677 29.802208575885935 21.789556516264287 74-75 19.453745409909395 29.60081555377558 30.175739355670906 20.76969968064412 76-77 20.171526515261824 28.60066314156034 30.809457047647175 20.418353295530657 78-79 19.372865311446173 28.613144940049878 31.21860874454942 20.79538100395453 80-81 19.152645932992463 30.20609033136084 30.315106206251713 20.32615752939498 82-83 19.477886336531025 29.654427079758445 29.910467107696135 20.957219476014394 84-85 19.513175201519477 28.834028151578256 29.853002471728207 21.799794175174064 86-87 20.0185379945684 28.946313035837907 30.407594587038712 20.627554382554983 88-89 20.951077662785288 27.785151461944995 30.669431590194396 20.59433928507532 90-91 19.909149427430016 28.44004898291382 31.223611661861444 20.427189927794725 92-93 19.72060660481226 29.301062964833864 30.47570859467777 20.502621835676106 94-95 19.71940056784017 29.250052327349913 29.77536482461911 21.2551822801908 96-97 20.02116404758457 28.477045788579236 29.30330085904561 22.19848930479058 98-99 20.46053247507079 28.27672725684006 29.43671319287398 21.826027075215173 100-101 21.32744780301502 27.991978815419145 29.80577333913011 20.87480004243573 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6265.0 1 3606.5 2 1152.5 3 1256.0 4 910.0 5 912.0 6 1653.5 7 3085.0 8 4366.5 9 4438.5 10 3474.5 11 2583.5 12 2453.5 13 2504.5 14 2472.0 15 2408.0 16 2413.0 17 2483.5 18 2498.5 19 2594.0 20 2876.0 21 3274.5 22 3765.0 23 4392.5 24 5008.5 25 6040.5 26 8130.5 27 10502.5 28 11922.5 29 14259.0 30 18477.5 31 22037.0 32 26802.5 33 33780.0 34 40486.5 35 45153.5 36 50641.5 37 56181.5 38 56342.5 39 54534.5 40 51801.0 41 48865.0 42 46218.0 43 44362.5 44 43727.0 45 42668.0 46 41642.5 47 42800.0 48 72495.5 49 71257.5 50 36446.0 51 26522.0 52 20392.0 53 16939.0 54 14406.0 55 12319.0 56 10425.5 57 8690.0 58 7249.5 59 5851.0 60 4424.0 61 3363.0 62 2459.0 63 1715.0 64 1243.0 65 910.0 66 686.0 67 561.5 68 436.0 69 295.0 70 226.0 71 202.0 72 162.5 73 139.0 74 125.0 75 108.0 76 80.5 77 61.0 78 54.0 79 47.0 80 31.5 81 12.0 82 8.5 83 7.0 84 5.5 85 4.0 86 2.5 87 1.0 88 0.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 1.5 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16373199631292437 2 0.094827079301112 3 0.08770469605229485 4 0.10401992279667832 5 0.12406197798521029 6 0.03884183422901444 7 0.041906115394203206 8 0.04588139906796162 9 0.046129854297571514 10-11 0.04447348610017218 12-13 0.04935977228250023 14-15 0.04658535555185633 16-17 0.046129854297571514 18-19 0.05014654717626491 20-21 0.051430232529249394 22-23 0.06550936220714375 24-25 0.08360518476373151 26-27 0.08579987262528561 28-29 0.07519911616192987 30-31 0.06335608355052462 32-33 0.050063728766394935 34-35 0.29566172323578155 36-37 0.47086406931569635 38-39 0.4479233697817155 40-41 0.42423730455890496 42-43 0.454176159726898 44-45 0.46361745845207425 46-47 0.4639901412964891 48-49 0.48080227850009233 50-51 0.4693733379380369 52-53 0.4762472659572442 54-55 0.4670130132567429 56-57 0.44461063338691686 58-59 0.4249412610427997 60-61 0.3948781782600018 62-63 0.4524369731196287 64-65 0.4900365312005936 66-67 0.49078189688942336 68-69 0.5023350650662837 70-71 0.49985051277018466 72-73 0.4900779404055286 74-75 0.4822930098777518 76-77 0.46428000573103395 78-79 0.4385234802614743 80-81 0.44245735473029774 82-83 0.4555426634897525 84-85 0.4528510651689786 86-87 0.464197187321164 88-89 0.47537767265360953 90-91 0.48179609941853196 92-93 0.4821687822629468 94-95 0.48784184333903957 96-97 0.48821452618345434 98-99 0.4810507337297022 100-101 0.4673856961011577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1207461.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.991825210468164 #Duplication Level Percentage of deduplicated Percentage of total 1 70.81547182111578 23.36331668519086 2 12.533820946090557 8.27027259745449 3 5.354493234725 5.299635146720445 4 2.8685918289343677 3.785603208815195 5 1.8163337261570742 2.996208240862628 6 1.2390673300776203 2.452745566675339 7 0.8688364280965564 2.006514970057436 8 0.613725747299281 1.6198346065649467 9 0.4870874222625098 1.4462912787752003 >10 2.8565220131184184 17.711877919053325 >50 0.31727565501428256 7.3389025755595885 >100 0.2141926568784901 13.010021037796276 >500 0.0075419949465368894 1.6681172961123987 >1k 0.005279396462575823 4.292300029391048 >5k 0.0012569991577561482 2.771071940290137 >10k+ 5.027996631024593E-4 1.9672869006806875 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13314 1.1026443090087383 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10405 0.8617255546970046 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8165 0.6762123165882791 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8099 0.6707463015368612 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6537 0.541383945319973 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5453 0.45160878902092905 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5156 0.4270117212895489 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4881 0.404236658575308 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4738 0.39239362596390276 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4438 0.36754810300291274 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3964 0.32829217672454847 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3458 0.2863860613303453 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3259 0.26990519776622185 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2800 0.23189154763590705 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2695 0.22319561459956055 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2312 0.1914761636193633 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2248 0.1861757853876854 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2068 0.17126847161109138 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1992 0.1649742724609739 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1851 0.15329687666930858 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1558 0.1290310825774083 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1459 0.12083206000028159 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1449 0.12000387590158192 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1442 0.11942414703249216 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1431 0.11851314452392252 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1331 0.11023130353692583 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1215 0.10062436799200968 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.140920493498341E-5 0.0 12-13 0.0 0.0 0.0 8.281840986996682E-5 0.0 14-15 0.0 0.0 0.0 8.281840986996682E-5 0.0 16-17 0.0 0.0 0.0 1.6563681973993363E-4 0.0 18-19 0.0 0.0 0.0 3.726828444148507E-4 0.0 20-21 0.0 0.0 0.0 7.453656888297013E-4 0.0 22-23 0.0 0.0 0.0 0.001904823427009237 0.0 24-25 0.0 0.0 0.0 0.003975283673758407 0.0 26-27 0.0 0.0 0.0 0.00766070291297193 0.0 28-29 0.0 0.0 0.0 0.017474684482562997 0.0 30-31 0.0 0.0 0.0 0.04534307940380683 0.0 32-33 0.0 0.0 0.0 0.09217689018527306 0.0 34-35 0.0 0.0 0.0 0.17275920298875078 0.0 36-37 0.0 0.0 0.0 0.2912309383077383 0.0 38-39 0.0 0.0 0.0 0.44440358736224195 0.0 40-41 0.0 0.0 0.0 0.6161689694325531 0.0 42-43 0.0 0.0 0.0 0.7861951648955949 0.0 44-45 0.0 0.0 0.0 0.9852078038131252 0.0 46-47 0.0 0.0 0.0 1.2313441179466666 0.0 48-49 0.0 0.0 0.0 1.5067981491741764 0.0 50-51 0.0 0.0 0.0 1.7837843209842803 0.0 52-53 0.0 0.0 0.0 2.041266757270007 0.0 54-55 0.0 0.0 0.0 2.317507563391281 0.0 56-57 0.0 0.0 0.0 2.6413689551877866 0.0 58-59 0.0 0.0 0.0 3.0212570012613247 0.0 60-61 0.0 0.0 0.0 3.478870124997826 0.0 62-63 0.0 0.0 0.0 3.8995048287273875 0.0 64-65 0.0 0.0 0.0 4.285231572696757 0.0 66-67 0.0 0.0 0.0 4.726819334123421 0.0 68-69 0.0 0.0 0.0 5.221162422637253 0.0 70-71 0.0 0.0 0.0 5.782339967916148 0.0 72-73 0.0 0.0 0.0 6.370888997657067 0.0 74-75 0.0 0.0 0.0 6.949044316959306 0.0 76-77 0.0 0.0 0.0 7.536102615322566 0.0 78-79 0.0 0.0 0.0 8.19583406834672 0.0 80-81 0.0 0.0 0.0 8.916726917059847 0.0 82-83 0.0 0.0 0.0 9.636915809289079 0.0 84-85 0.0 0.0 0.0 10.428204306391676 0.0 86-87 0.0 0.0 0.0 11.275519457771306 0.0 88-89 0.0 0.0 0.0 12.08320600002816 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 235 0.0 28.238667 1 CGCCGTA 45 8.878758E-4 26.425238 94-95 GAGTACT 5385 0.0 23.033945 12-13 CATTCCG 260 6.511982E-10 21.850008 9 GCGTGCA 110 0.0029337294 21.518946 9 GTACATG 5995 0.0 21.506205 1 TCGCCGT 100 5.320235E-7 21.404442 94-95 GTACTTT 5820 0.0 21.027191 14-15 GGTCGCC 160 1.8189894E-11 20.810307 92-93 AGTACTT 5665 0.0 20.68369 12-13 CATGGGG 1470 0.0 20.627586 4 ACATGGG 5930 0.0 19.97428 3 TACATGG 6225 0.0 19.788816 2 GCACGCC 170 8.160931E-5 19.493635 9 GATTGGC 515 0.0 19.304377 9 GTATAGG 645 0.0 19.107225 1 GCGCACG 125 0.0061487295 18.936672 7 CATATAG 435 0.0 18.515926 3 ACTTTTT 6815 0.0 18.478575 16-17 GTCTAAG 360 9.822543E-11 18.433575 1 >>END_MODULE