##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938723_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1207461 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.957729483602368 31.0 31.0 34.0 30.0 34.0 2 32.186137688919146 33.0 31.0 34.0 30.0 34.0 3 32.26552990117279 34.0 31.0 34.0 30.0 34.0 4 35.896425640248424 37.0 35.0 37.0 35.0 37.0 5 35.80449057981997 37.0 35.0 37.0 35.0 37.0 6 35.846331268670376 37.0 35.0 37.0 35.0 37.0 7 35.78910954473892 37.0 35.0 37.0 33.0 37.0 8 35.77289121553408 37.0 35.0 37.0 33.0 37.0 9 37.40390952585632 39.0 37.0 39.0 34.0 39.0 10-11 37.336904049074874 39.0 37.0 39.0 34.0 39.0 12-13 37.28232174786598 39.0 37.0 39.0 33.5 39.0 14-15 38.43752551842254 40.0 38.0 41.0 33.0 41.0 16-17 38.370021060721626 40.0 38.0 41.0 33.0 41.0 18-19 38.448521732793026 40.0 38.0 41.0 33.5 41.0 20-21 38.53591006251962 40.0 38.0 41.0 34.0 41.0 22-23 38.499003694529264 40.0 38.0 41.0 34.0 41.0 24-25 38.42490109411401 40.0 38.0 41.0 34.0 41.0 26-27 38.209405521172116 40.0 37.5 41.0 33.5 41.0 28-29 38.045648679336225 40.0 37.0 41.0 33.0 41.0 30-31 37.83739184950901 40.0 37.0 41.0 33.0 41.0 32-33 37.61935913458075 40.0 36.0 41.0 33.0 41.0 34-35 37.582634967092105 40.0 36.0 41.0 33.0 41.0 36-37 37.45275996491812 40.0 36.0 41.0 32.0 41.0 38-39 37.32728676122873 40.0 36.0 41.0 32.0 41.0 40-41 37.16980879713714 40.0 36.0 41.0 31.0 41.0 42-43 37.03027426972797 40.0 36.0 41.0 31.0 41.0 44-45 36.80287479264341 40.0 36.0 41.0 30.5 41.0 46-47 36.5033756783863 40.0 36.0 41.0 30.0 41.0 48-49 36.24025786340097 39.5 35.0 41.0 30.0 41.0 50-51 36.041397610357606 39.0 35.0 41.0 29.0 41.0 52-53 35.82553225321563 39.0 35.0 41.0 28.0 41.0 54-55 35.64884497304675 39.0 35.0 40.5 28.0 41.0 56-57 35.476173557572466 39.0 35.0 40.0 27.5 41.0 58-59 35.251495493436224 39.0 35.0 40.0 27.0 41.0 60-61 34.926429507868164 38.0 34.0 40.0 26.0 41.0 62-63 34.56093116050953 38.0 34.0 40.0 26.0 41.0 64-65 34.16162633824198 37.5 34.0 40.0 25.0 41.0 66-67 33.79452089963982 37.0 33.0 39.5 23.0 41.0 68-69 33.38795952829946 36.5 33.0 39.0 22.5 40.5 70-71 32.909811993927754 36.0 32.0 39.0 22.0 40.0 72-73 32.530721075049215 35.5 32.0 38.5 21.5 40.0 74-75 32.088563937054694 35.0 32.0 37.5 20.0 39.5 76-77 29.927074249189 33.5 29.0 35.5 15.0 38.5 78-79 30.969974185501645 34.5 31.0 36.0 18.0 39.0 80-81 31.02151291014782 35.0 31.0 36.0 17.5 38.5 82-83 30.696316485584212 34.5 31.0 36.0 12.5 37.0 84-85 30.285696598068178 34.0 31.0 35.0 7.5 37.0 86-87 29.718641430240808 34.0 30.0 35.0 2.0 36.5 88-89 29.152696029105705 34.0 29.0 35.0 2.0 36.0 90-91 28.69292465760799 34.0 29.0 35.0 2.0 36.0 92-93 28.447983413128874 34.0 29.0 35.0 2.0 35.0 94-95 28.09997548575068 34.0 29.0 35.0 2.0 35.0 96-97 27.578347043921088 33.5 28.0 35.0 2.0 35.0 98-99 26.858382589582604 33.0 26.5 35.0 2.0 35.0 100-101 23.72563503086228 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.008538588846363382 1101 2 0.09531463770659343 1101 3 0.11806729301205365 1101 4 0.0689491713676631 1101 5 0.11956079167904932 1101 6 0.21346774678234937 1101 7 0.06421738184027248 1101 8 0.17975082090348593 1101 9 0.26297666638685513 1101 10-11 0.15036034477925853 1101 12-13 0.041486914488423565 1101 14-15 0.11626475467861752 1101 16-17 0.12972895251616734 1101 18-19 0.2288271174900558 1101 20-21 0.3136024395230308 1101 22-23 0.15750478698682713 1101 24-25 0.2196594069200728 1101 26-27 0.20874866387318036 1101 28-29 0.4119820597390955 1101 30-31 0.3172349407997501 1101 32-33 0.3800047785192717 1101 34-35 0.4365994619763569 1101 36-37 0.4101703369579397 1101 38-39 0.49223012092686247 1101 40-41 0.5678419906733225 1101 42-43 0.6373519918602568 1101 44-45 0.7141492378420438 1101 46-47 0.46717843984604457 1101 48-49 0.16271782578201766 1101 50-51 0.24489125242982368 1101 52-53 0.3993352362456619 1101 54-55 0.3548680761132559 1101 56-57 0.31243471655383814 1101 58-59 0.5417150949794447 1101 60-61 0.5594427373248578 1101 62-63 0.5429267459253566 1101 64-65 0.517233107545664 1101 66-67 0.3731527839897879 1101 68-69 0.5157693719559333 1101 70-71 0.5410815836079976 1101 72-73 0.4700187904813973 1101 74-75 0.5604262612832756 1101 76-77 0.34812031048953074 1101 78-79 0.46338034883757473 1101 80-81 0.73605468340347 1101 82-83 0.7324018249081625 1101 84-85 0.7438748242001729 1101 86-87 0.7763093872405129 1101 88-89 1.0072032568998814 1101 90-91 0.937405523953192 1101 92-93 0.9110677786710148 1101 94-95 0.8298215654747487 1101 96-97 0.7939240584524185 1101 98-99 0.9383974952539447 1101 100-101 1.549002969740421 1102 1 0.011873877153561807 1102 2 0.08851364375671977 1102 3 0.20167020695204485 1102 4 0.11722827989958517 1102 5 0.06638678871571813 1102 6 0.03927263010127291 1102 7 0.016642063899780624 1102 8 -0.008997018335925588 1102 9 0.12370252760428713 1102 10-11 0.20980788459898747 1102 12-13 0.21600151505639786 1102 14-15 0.276066119044593 1102 16-17 0.23514937479051667 1102 18-19 0.15400075003728375 1102 20-21 0.1312099373622715 1102 22-23 0.174926690925588 1102 24-25 -0.021049708402799183 1102 26-27 0.2647910765167225 1102 28-29 0.12328245176280461 1102 30-31 0.20822186819820132 1102 32-33 0.2648848563066579 1102 34-35 0.3054262461472419 1102 36-37 0.36672052833959157 1102 38-39 0.3771132855150796 1102 40-41 0.4421733124414473 1102 42-43 0.25273737894507775 1102 44-45 0.29745196040038735 1102 46-47 0.3157967106202122 1102 48-49 0.34796330361878347 1102 50-51 0.5076038558376581 1102 52-53 0.2165186848195617 1102 54-55 0.33580399412782924 1102 56-57 0.29467673717366694 1102 58-59 0.4280621748658504 1102 60-61 0.2874801493510333 1102 62-63 0.26152009078274574 1102 64-65 0.3582771407596397 1102 66-67 0.23577201167221773 1102 68-69 0.19738631540574403 1102 70-71 0.34598766769245515 1102 72-73 0.37286752180624916 1102 74-75 0.5862238134502107 1102 76-77 0.4411970020563878 1102 78-79 0.41001805102303024 1102 80-81 0.43329045325160465 1102 82-83 0.460452355837905 1102 84-85 0.5562725739223602 1102 86-87 0.3307539945994442 1102 88-89 0.47184935518361826 1102 90-91 0.5117391608219428 1102 92-93 0.43819111179856307 1102 94-95 0.7212217383355899 1102 96-97 0.7211774230653347 1102 98-99 0.7848800856972105 1102 100-101 1.1139546613072824 1103 1 -0.002611386607735966 1103 2 -0.02575494992227334 1103 3 -0.0717707040424358 1103 4 -0.04958912023763418 1103 5 -0.03082887449669869 1103 6 0.007260702108808914 1103 7 -0.015771572504355902 1103 8 0.01817436042239251 1103 9 0.023374923627891064 1103 10-11 -0.09677545541710231 1103 12-13 -0.026354887180694675 1103 14-15 0.029406016337922836 1103 16-17 -0.07914439063553402 1103 18-19 -0.05409200376669787 1103 20-21 3.829378048152421E-4 1103 22-23 -0.06847704904654961 1103 24-25 -0.1343277678467416 1103 26-27 -0.021596206033535736 1103 28-29 0.02895708428499688 1103 30-31 0.006420937363323276 1103 32-33 -0.02276584003742954 1103 34-35 -0.06219932300155051 1103 36-37 -0.11042367384225571 1103 38-39 0.02702700359745336 1103 40-41 0.09217464899437999 1103 42-43 0.11379713177189643 1103 44-45 0.19574288045372867 1103 46-47 0.042627936655080134 1103 48-49 0.16939277669021635 1103 50-51 0.13679877574843147 1103 52-53 0.00695980669240015 1103 54-55 0.04579836575488372 1103 56-57 -0.10357074490909923 1103 58-59 -0.19210915637057724 1103 60-61 0.04158559446771193 1103 62-63 -0.06579318534612355 1103 64-65 0.03363437165955929 1103 66-67 -0.056279285229649645 1103 68-69 -0.0048200364878567825 1103 70-71 0.012665101035587156 1103 72-73 0.1159211591608269 1103 74-75 0.21162440806776317 1103 76-77 0.18270395064662281 1103 78-79 0.10719785006487115 1103 80-81 0.14762193917175992 1103 82-83 0.05165501587527288 1103 84-85 0.1849109680391514 1103 86-87 0.42347586981292196 1103 88-89 0.5276407875822606 1103 90-91 0.4673622618029718 1103 92-93 0.6053255719945305 1103 94-95 0.4094607407324773 1103 96-97 0.45118964509944703 1103 98-99 0.8735288158234091 1103 100-101 1.2566643590384103 1104 1 0.047432317069361574 1104 2 -0.010282052188877344 1104 3 -0.037681835907804384 1104 4 -0.008010758945921737 1104 5 -0.008596873826178353 1104 6 0.008829245040189448 1104 7 -0.07798645091234135 1104 8 -0.10838071219755818 1104 9 -0.03669667856088665 1104 10-11 0.036869491120725684 1104 12-13 0.09852088661386205 1104 14-15 0.24651301251834212 1104 16-17 0.21343975390988845 1104 18-19 0.06987676905799844 1104 20-21 0.11435374614323734 1104 22-23 0.0762330099833406 1104 24-25 0.23737499925456973 1104 26-27 0.37090023181743703 1104 28-29 0.26279869736976025 1104 30-31 0.1799200453293679 1104 32-33 0.2554185415854775 1104 34-35 0.33351801598332287 1104 36-37 0.302330615703589 1104 38-39 0.4937762881742529 1104 40-41 0.438824133530467 1104 42-43 0.454708263637265 1104 44-45 0.3568103876678279 1104 46-47 0.38955431092618653 1104 48-49 0.49177923693184766 1104 50-51 0.42031590399777485 1104 52-53 0.43171764042684657 1104 54-55 0.5562618043362519 1104 56-57 0.6114438628953067 1104 58-59 0.575513004387112 1104 60-61 0.520244131053083 1104 62-63 0.20290061352117306 1104 64-65 0.5255192573714424 1104 66-67 0.4009075749305566 1104 68-69 0.3629928040116823 1104 70-71 0.6496072804721109 1104 72-73 0.6777169612131004 1104 74-75 0.632366592293728 1104 76-77 0.5128088993397029 1104 78-79 0.7853097913462861 1104 80-81 0.602300328002773 1104 82-83 0.6779810932008452 1104 84-85 0.7213900681026111 1104 86-87 0.8161504151150751 1104 88-89 0.9016065670044107 1104 90-91 0.9717805239531891 1104 92-93 0.9863617682180568 1104 94-95 0.8336543180879836 1104 96-97 0.7611714452119998 1104 98-99 0.6983713628497625 1104 100-101 1.0339986143397262 1105 1 -0.035768492960720266 1105 2 -0.06253792639597577 1105 3 -0.12405724911248939 1105 4 -0.15740919249069663 1105 5 -0.10663731576905633 1105 6 -0.19459849253389194 1105 7 -0.0830506279277401 1105 8 -0.14829405089137992 1105 9 -0.046402149241963286 1105 10-11 -0.05572558318166898 1105 12-13 -0.12946088770937791 1105 14-15 -0.24743335276948386 1105 16-17 -0.1302832574960533 1105 18-19 -0.06808222316928436 1105 20-21 -0.1250994835538961 1105 22-23 -0.11306908358704248 1105 24-25 -0.07130899967333448 1105 26-27 -0.20933741671289852 1105 28-29 -0.11167865454661552 1105 30-31 -0.30052681133700077 1105 32-33 -0.1477195804550817 1105 34-35 -0.12441625841437087 1105 36-37 -0.20759523080763387 1105 38-39 -0.18637946393272387 1105 40-41 -0.040876721170384656 1105 42-43 -0.16318982743155885 1105 44-45 -0.20267768523488172 1105 46-47 -0.295386425843823 1105 48-49 -0.4957032670140791 1105 50-51 -0.44893124451766653 1105 52-53 -0.3256113449509215 1105 54-55 -0.38566916442398025 1105 56-57 -0.4167128719687483 1105 58-59 -0.2890846608203077 1105 60-61 -0.49559389271177423 1105 62-63 -0.2672228267242218 1105 64-65 -0.3115634431258911 1105 66-67 -0.1495540230920156 1105 68-69 -0.37835074403918867 1105 70-71 -0.4533208889194782 1105 72-73 -0.6196370397994357 1105 74-75 -0.5833046605726437 1105 76-77 -0.6467171437229275 1105 78-79 -0.7421523129450875 1105 80-81 -0.5265674167186276 1105 82-83 -0.5916747929184574 1105 84-85 -0.613928136727786 1105 86-87 -0.6154526701465493 1105 88-89 -0.3110278175811949 1105 90-91 -0.1575540303813625 1105 92-93 -0.18658560960737347 1105 94-95 -0.0603493746961945 1105 96-97 -0.34139340186504086 1105 98-99 -0.20822948887304094 1105 100-101 0.3585191480066001 1106 1 -0.06699189531382643 1106 2 -0.0871382911637042 1106 3 -0.06346289228382318 1106 4 0.0017868880442932777 1106 5 -0.10217785147695935 1106 6 -0.13143288083271187 1106 7 -0.03030592957634326 1106 8 -0.04428160587475105 1106 9 0.016985184019844723 1106 10-11 -0.05585790088720444 1106 12-13 0.046915037620529176 1106 14-15 0.1055616763761762 1106 16-17 -0.06154420946829475 1106 18-19 -0.29278450833182035 1106 20-21 -0.24909369018408256 1106 22-23 -0.22187357523672802 1106 24-25 -0.17879210234471543 1106 26-27 -0.0985660402511428 1106 28-29 -0.11188785956753833 1106 30-31 -0.20110972447221798 1106 32-33 -0.0667786942840749 1106 34-35 0.031509429101276965 1106 36-37 -0.014037672605716978 1106 38-39 0.06485788542178028 1106 40-41 0.2280500749530603 1106 42-43 0.1221211191764553 1106 44-45 0.07081764788387801 1106 46-47 0.20165430655256245 1106 48-49 0.3122751330145377 1106 50-51 0.198942731808188 1106 52-53 0.32336766302390885 1106 54-55 0.3187652667886951 1106 56-57 0.2782054129076954 1106 58-59 0.12346942851201703 1106 60-61 0.27463998777314913 1106 62-63 0.11797904718058305 1106 64-65 0.25778712727070996 1106 66-67 0.35716914681106715 1106 68-69 0.27042978737733137 1106 70-71 0.34741937500071174 1106 72-73 0.6015284288555733 1106 74-75 0.7462021877626555 1106 76-77 0.6063297396586869 1106 78-79 0.5307779878094792 1106 80-81 0.45789419326599656 1106 82-83 0.7075026916146783 1106 84-85 0.36463281882061693 1106 86-87 0.4160228510779298 1106 88-89 0.6682948588126152 1106 90-91 0.7925067642400982 1106 92-93 1.0544722168061007 1106 94-95 0.8471855822111465 1106 96-97 0.739754901249789 1106 98-99 0.8948156064314681 1106 100-101 1.351472336148074 1107 1 -0.03214754037711032 1107 2 -0.06316565274983788 1107 3 -0.11992915037858154 1107 4 -0.1493303188516606 1107 5 -0.04749981887222532 1107 6 -0.046983498030932935 1107 7 -0.10060923345314876 1107 8 -0.0055448222500373845 1107 9 -0.029765627626190394 1107 10-11 -0.1183989172242974 1107 12-13 -0.07338045362063639 1107 14-15 -0.1766775986112421 1107 16-17 -0.15758580741270833 1107 18-19 0.027497093779331294 1107 20-21 0.031379929149579766 1107 22-23 -0.15002336950398387 1107 24-25 -0.27298470985526535 1107 26-27 -0.07131209487014445 1107 28-29 -0.14093176657981843 1107 30-31 -0.24507055712556536 1107 32-33 -0.15824729616952027 1107 34-35 -0.24726798022874164 1107 36-37 -0.06517050477294362 1107 38-39 0.09513098044079271 1107 40-41 -0.10360325236164414 1107 42-43 -0.20806516877993175 1107 44-45 -0.31175843850650864 1107 46-47 -0.3497024878362325 1107 48-49 -0.45649342199510556 1107 50-51 -0.45233940850674514 1107 52-53 -0.37055584259250907 1107 54-55 -0.3732884146988269 1107 56-57 -0.45231595031041394 1107 58-59 -0.543687987415332 1107 60-61 -0.5604890943289647 1107 62-63 -0.6124155777260967 1107 64-65 -0.4468136470482875 1107 66-67 -0.39001222786557577 1107 68-69 -0.21502308506836698 1107 70-71 -0.5873138668958546 1107 72-73 -0.5189649824883773 1107 74-75 -0.7025868537137612 1107 76-77 -0.6486694790777499 1107 78-79 -0.5011461775406119 1107 80-81 -0.5294684345574048 1107 82-83 -0.44136447621809793 1107 84-85 -0.41962732458464913 1107 86-87 -0.4052796020896601 1107 88-89 -0.17442463284522702 1107 90-91 0.15543494007648562 1107 92-93 0.14709882490693715 1107 94-95 0.0723526858066954 1107 96-97 0.1543419600528928 1107 98-99 0.19276175132981876 1107 100-101 0.6178641137831029 1108 1 -0.2005370760159586 1108 2 -0.1967879158354222 1108 3 -0.2264608312524814 1108 4 -0.11211990370882319 1108 5 -0.12319485670880681 1108 6 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-1.1552976858928332 2316 64-65 -1.169617541804982 2316 66-67 -1.0941666153608622 2316 68-69 -1.1358032741479605 2316 70-71 -1.2056919553530427 2316 72-73 -1.3822032062718534 2316 74-75 -1.35872958287257 2316 76-77 -1.4682352089909934 2316 78-79 -1.341012914149438 2316 80-81 -1.5554530276354903 2316 82-83 -1.7519427367801477 2316 84-85 -1.824865029695932 2316 86-87 -2.031107414707538 2316 88-89 -2.16484422686635 2316 90-91 -2.717190661111747 2316 92-93 -2.8544050297705432 2316 94-95 -2.7924085481616174 2316 96-97 -2.7999476947943336 2316 98-99 -3.097276206044757 2316 100-101 -3.8270826633764585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 16.0 5 47.0 6 158.0 7 274.0 8 541.0 9 872.0 10 1566.0 11 2314.0 12 3139.0 13 4510.0 14 5085.0 15 6873.0 16 8985.0 17 12041.0 18 11079.0 19 7475.0 20 6634.0 21 6929.0 22 7492.0 23 7983.0 24 9696.0 25 11490.0 26 13573.0 27 16372.0 28 19938.0 29 24333.0 30 29325.0 31 35331.0 32 43718.0 33 54743.0 34 71850.0 35 101744.0 36 143026.0 37 242584.0 38 263456.0 39 32262.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.97307695185863 22.770346731942727 15.97364900427127 21.282927311927377 2 14.117226146434545 23.742547378341825 40.643548735735564 21.496677739488064 3 17.924057174517436 29.159202657477135 32.160044920705516 20.756695247299913 4 11.561367199437496 19.61943284296553 40.52619504894982 28.293004908647152 5 11.118619980272655 40.057277212266065 36.60234160772066 12.221761199740614 6 25.90725497552302 40.89399160718234 19.12608357537014 14.072669841924501 7 23.876547565511434 35.6798273401791 23.901972817341512 16.54165227696795 8 22.393104207920587 36.112801986979285 22.41049607399328 19.083597731106845 9 24.441369120824607 19.050139093519377 23.53227143568198 32.97622034997404 10-11 21.661776239563846 28.35839004323949 29.850280878637076 20.12955283855959 12-13 24.64257644760369 26.613074873639814 29.110588250883467 19.63376042787303 14-15 22.160250841230784 28.070689059734633 28.638204093396286 21.130856005638297 16-17 18.6300817351133 31.291487671820562 30.125795383113157 19.95263520995298 18-19 18.26811510784217 30.955709469224214 32.20043082208174 18.57574460085187 20-21 19.317104237735215 29.815704192516364 31.31869269483652 19.5484988749119 22-23 19.164387089934994 29.38459295993825 30.625751059454508 20.82526889067225 24-25 19.391806443437925 29.979394779624354 31.506856122061084 19.12194265487664 26-27 20.074553256052585 28.90251346008436 32.047672012051756 18.9752612718113 28-29 18.806735786911545 28.74846475372704 33.12641153627322 19.3183879230882 30-31 18.93982497157258 30.456925730934582 32.16932058261095 18.43392871488189 32-33 18.78491313591081 30.35820618636958 31.35550547802372 19.501375199695893 34-35 19.332463947329952 29.208015302739724 31.50947248368452 19.95004826624581 36-37 20.151973619361044 29.054445271853613 31.625083143576845 19.168497965208502 38-39 20.28657616892911 28.651446216132715 32.2321367190056 18.82984089593257 40-41 19.014029679410356 29.06907498867372 33.05903166306241 18.85786366885352 42-43 18.63929644999108 29.985767167086504 31.93393017091409 19.441006212008336 44-45 18.86407182826743 30.424689351936397 30.89026287532543 19.82097594447074 46-47 19.07983604941707 29.84304771180608 29.891870134839333 21.185246103937512 48-49 19.384891106213782 29.500828598190747 30.606495779159744 20.507784516435727 50-51 20.30276942341879 29.695843359599355 30.480345619934845 19.52104159704701 52-53 19.080781905171264 29.96900106918567 31.50507552624888 19.445141499394182 54-55 18.611698431667772 29.712056952564097 31.419110016803852 20.257134598964274 56-57 19.419053700285144 30.825384836446062 29.653669973605773 20.10189148966302 58-59 18.82508542740203 29.76045543613111 30.57029726913897 20.84416186732789 60-61 19.195588736469986 29.58103183184128 30.43646779236816 20.78691163932057 62-63 20.49826039930068 29.160776207264664 30.442763782846814 19.898199610587834 64-65 19.999031024604523 29.07588733714795 30.93603023203234 19.989051406215193 66-67 18.809648334929683 29.532735126298043 30.78029039088022 20.87732614789205 68-69 18.688776034161194 30.355740849325684 30.648713157955758 20.306769958557357 70-71 19.088333009644813 29.95523988218014 29.802312585382335 21.15411452279271 72-73 18.93415191049649 29.399293227690173 29.437389696230355 22.229165165582987 74-75 19.266336552484926 29.734128058794447 29.727709632029526 21.271825756691108 76-77 19.85264462484482 28.75643254916509 30.530707326525835 20.860215499464257 78-79 19.05214797496884 28.666701738238213 30.892075799331515 21.38907448746143 80-81 18.796888179778968 30.225253745360614 30.03779419699444 20.940063877865985 82-83 19.040159475130043 29.87939154970637 29.559174167944143 21.521274807219445 84-85 19.109105801346793 28.878075565173532 29.45838416313239 22.55443447034728 86-87 19.395194384919506 29.0813658308384 30.082891613833823 21.44054817040827 88-89 20.284097258775528 27.85610513725648 30.42662364882016 21.43317395514783 90-91 19.308984447997343 28.404725815493865 30.90388488611117 21.382404850397624 92-93 19.019247826637876 29.287198509931166 30.172113219391765 21.521440444039186 94-95 18.90748438251836 29.160817616469597 29.593378171220436 22.338319829791605 96-97 19.108981573731988 28.571895903884265 29.003421228511726 23.315701293872017 98-99 19.434018518211786 28.29244861113412 29.32362148642605 22.94991138422805 100-101 19.98835565442299 28.160516392770568 29.79403753189565 22.057090420910793 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5619.0 1 3295.5 2 1066.0 3 1083.0 4 841.5 5 867.0 6 1552.5 7 2984.5 8 4133.5 9 4107.5 10 3056.0 11 2143.5 12 2048.0 13 2038.5 14 1961.0 15 1844.0 16 1829.0 17 1938.0 18 2023.0 19 2168.0 20 2551.5 21 2949.5 22 3423.0 23 4037.5 24 4678.0 25 5796.0 26 7908.5 27 10126.0 28 11614.0 29 13941.0 30 18151.0 31 22091.5 32 27017.0 33 34179.5 34 40987.0 35 45596.0 36 51377.0 37 56726.0 38 56859.5 39 55283.5 40 52641.0 41 49511.0 42 46231.0 43 44615.5 44 44384.5 45 43208.0 46 41857.0 47 42768.0 48 72279.5 49 71815.5 50 39305.5 51 29383.0 52 21039.0 53 16657.5 54 14038.0 55 12015.0 56 10107.5 57 8361.0 58 6950.5 59 5630.5 60 4240.0 61 3106.5 62 2230.5 63 1603.5 64 1159.5 65 837.5 66 602.5 67 448.5 68 318.5 69 194.5 70 169.0 71 148.5 72 112.0 73 99.0 74 86.0 75 74.5 76 55.5 77 30.0 78 23.0 79 18.5 80 8.5 81 5.5 82 4.0 83 3.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39537508871922156 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.3127363947986726E-4 16-17 0.0019876418368792036 18-19 1.6563681973993363E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.6563681973993363E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007909158142581831 36-37 0.017557502892432963 38-39 0.011428940562055422 40-41 0.007619293708036947 42-43 0.003436964009603623 44-45 0.0021118694516841538 46-47 0.0019876418368792036 48-49 0.0 50-51 6.211380740247511E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.4845522960990045E-4 60-61 5.383196641547842E-4 62-63 0.0 64-65 0.0 66-67 0.001614958992464353 68-69 7.453656888297014E-4 70-71 0.0020290510418141867 72-73 0.0 74-75 0.0 76-77 0.09884377217980539 78-79 0.03358286520227154 80-81 4.140920493498341E-5 82-83 0.0 84-85 0.0 86-87 0.15325546746437357 88-89 0.3254349415840346 90-91 0.28456405631320597 92-93 0.0 94-95 0.0 96-97 0.0 98-99 8.281840986996682E-5 100-101 6.625472789597345E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1207461.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.96564073075257 #Duplication Level Percentage of deduplicated Percentage of total 1 68.4151089254601 21.18517683541101 2 13.408185465395015 8.303861079454409 3 5.721299103198931 5.314910776285049 4 3.237621348750968 4.010200780305482 5 1.9820696880604811 3.0688028931897837 6 1.297693399562887 2.411034455371998 7 0.9379079830346306 2.03300451488086 8 0.7196186603504675 1.782676231964643 9 0.5354082705030876 1.492133413380498 >10 3.1522431075501407 18.328646212937908 >50 0.3386560914156875 7.345796022435776 >100 0.2376162918349581 13.678982890792673 >500 0.008820402276252958 1.8414715752443487 >1k 0.005880268184168638 4.493723659774222 >5k 0.001336424587311054 2.7292249176898915 >10k+ 5.345698349244216E-4 1.9803537408814569 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13232 1.095853199399401 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10695 0.885742893559295 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7647 0.6333123802756363 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7576 0.6274322731748686 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6515 0.5395619403028338 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5637 0.4668473764370029 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5600 0.4637830952718141 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4853 0.40191774309894895 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4774 0.39537508871922156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4715 0.39048880253689355 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4313 0.3571958017691669 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3255 0.26957392412674197 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3047 0.2523476948737889 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3032 0.2511054187257394 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2773 0.22965545056941797 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2430 0.20124873598401935 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2369 0.19619681298195138 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2226 0.18435378037054612 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2085 0.1726763845788808 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2027 0.16787291680642275 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1582 0.1310187244142875 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1519 0.1258011645924796 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1512 0.1252214357233898 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1462 0.1210805152298915 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1429 0.11834750770418259 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1429 0.11834750770418259 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1323 0.1095687562579661 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.281840986996682E-5 0.0 12-13 0.0 0.0 0.0 1.6563681973993363E-4 0.0 14-15 0.0 0.0 0.0 1.6563681973993363E-4 0.0 16-17 0.0 0.0 0.0 2.4845522960990045E-4 0.0 18-19 0.0 0.0 0.0 4.140920493498341E-4 0.0 20-21 0.0 0.0 0.0 6.625472789597345E-4 0.0 22-23 0.0 0.0 0.0 0.0018220050171392698 0.0 24-25 0.0 0.0 0.0 0.00389246526388844 0.0 26-27 0.0 0.0 0.0 0.007577884503101964 0.0 28-29 0.0 0.0 0.0 0.017309047662823065 0.0 30-31 0.0 0.0 0.0 0.04534307940380683 0.0 32-33 0.0 0.0 0.0 0.09296366507903775 0.0 34-35 0.0 0.0 0.0 0.17400147913680028 0.0 36-37 0.0 0.0 0.0 0.29350844457916236 0.0 38-39 0.0 0.0 0.0 0.44709518568301587 0.0 40-41 0.0 0.0 0.0 0.6204755267457913 8.281840986996682E-5 42-43 0.0 0.0 0.0 0.7922409088161025 8.281840986996682E-5 44-45 0.0 0.0 0.0 0.9925786422915523 8.281840986996682E-5 46-47 0.0 0.0 0.0 1.240371324622493 8.281840986996682E-5 48-49 0.0 0.0 0.0 1.5184341357609066 8.281840986996682E-5 50-51 0.0 0.0 0.0 1.7972837217930848 8.281840986996682E-5 52-53 0.0 0.0 0.0 2.056049843431796 8.281840986996682E-5 54-55 0.0 0.0 0.0 2.33353292570112 8.281840986996682E-5 56-57 0.0 0.0 0.0 2.6588022304654144 8.281840986996682E-5 58-59 0.0 0.0 0.0 3.0404294631462214 8.281840986996682E-5 60-61 0.0 0.0 0.0 3.5000302287196026 8.281840986996682E-5 62-63 0.0 0.0 0.0 3.9227353926959134 8.281840986996682E-5 64-65 0.0 0.0 0.0 4.3107810521416425 8.281840986996682E-5 66-67 0.0 0.0 0.0 4.755019002684144 8.281840986996682E-5 68-69 0.0 0.0 0.0 5.251639597469401 8.281840986996682E-5 70-71 0.0 0.0 0.0 5.81666819880725 8.281840986996682E-5 72-73 0.0 0.0 0.0 6.408944056992317 8.281840986996682E-5 74-75 0.0 0.0 0.0 6.9921098900916885 8.281840986996682E-5 76-77 0.0 0.0 0.0 7.583102062923771 8.281840986996682E-5 78-79 0.0 0.0 0.0 8.244738339374937 8.281840986996682E-5 80-81 0.0 0.0 0.0 8.966956282645981 8.281840986996682E-5 82-83 0.0 0.0 0.0 9.68801476817885 8.281840986996682E-5 84-85 0.0 0.0 0.0 10.479965812560405 8.281840986996682E-5 86-87 0.0 0.0 0.0 11.330676518744704 8.281840986996682E-5 88-89 0.0 0.0 0.0 12.13981238317428 8.281840986996682E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGCT 50 0.0016240777 37.960724 7 TAGACCG 60 0.0039710803 31.63394 4 GACCGGT 65 0.005873792 29.20056 6 CTTTGCG 195 1.5872502E-8 24.3338 2 CATATAG 415 0.0 24.011305 3 CTCCCGC 200 2.0769221E-8 23.725454 7 GAGTACT 5495 0.0 22.45268 12-13 AGTACTT 5770 0.0 21.58818 12-13 GTACTTT 5745 0.0 21.103931 14-15 CATGGGG 1455 0.0 20.871878 4 AGGCTTA 685 0.0 20.78142 6 GTACATG 6320 0.0 20.733006 1 CATGGGT 770 0.0 20.336103 4 ACTCCCG 210 8.6942964E-7 20.336103 6 GGCGTGC 120 0.0047922144 19.771212 8 ACATGGG 6235 0.0 19.406548 3 GGACTGA 245 1.7754246E-7 19.367718 6 CGCTGTA 135 2.3288521E-8 19.332253 10-11 GGACAAT 445 0.0 19.193624 6 TACATGG 6670 0.0 18.92345 2 >>END_MODULE