##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938722_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1201584 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66006121919067 33.0 31.0 34.0 30.0 34.0 2 31.735764624029613 33.0 31.0 34.0 30.0 34.0 3 31.730753738398647 33.0 31.0 34.0 30.0 34.0 4 35.27953434799398 37.0 35.0 37.0 33.0 37.0 5 35.32838902648504 37.0 35.0 37.0 33.0 37.0 6 35.39971237965885 37.0 35.0 37.0 33.0 37.0 7 35.333279238072414 37.0 35.0 37.0 33.0 37.0 8 35.31058502776335 37.0 35.0 37.0 33.0 37.0 9 36.886876822594175 39.0 37.0 39.0 33.0 39.0 10-11 36.78748010958867 39.0 37.0 39.0 33.0 39.0 12-13 36.709932472469674 39.0 37.0 39.0 32.0 39.0 14-15 37.833222229989744 40.0 38.0 41.0 32.5 41.0 16-17 37.7278047144436 40.0 37.5 41.0 32.0 41.0 18-19 37.74141133703512 40.0 37.5 41.0 32.0 41.0 20-21 37.66256042024528 40.0 37.0 41.0 32.0 41.0 22-23 37.68446400751009 40.0 37.5 41.0 32.0 41.0 24-25 37.634357647904764 40.0 37.0 41.0 32.0 41.0 26-27 37.470727805962795 40.0 37.0 41.0 32.0 41.0 28-29 37.31135276435106 40.0 37.0 41.0 31.5 41.0 30-31 37.11255184822701 40.0 36.5 41.0 31.0 41.0 32-33 36.93294809185209 39.5 36.0 41.0 31.0 41.0 34-35 36.67749487343373 39.0 35.5 41.0 30.0 41.0 36-37 36.460433893926684 39.0 35.0 41.0 30.0 41.0 38-39 36.28199360177898 39.0 35.0 41.0 30.0 41.0 40-41 36.16762914619369 39.0 35.0 41.0 30.0 41.0 42-43 36.17889552457423 39.0 35.0 41.0 30.0 41.0 44-45 36.01186475518982 39.0 35.0 41.0 29.5 41.0 46-47 35.69110440884699 39.0 35.0 41.0 27.0 41.0 48-49 35.420700508661895 39.0 35.0 41.0 26.0 41.0 50-51 34.33387511817734 38.0 34.0 40.0 24.0 40.5 52-53 34.40717253225742 38.0 34.5 39.5 24.0 40.5 54-55 34.96026536638304 39.0 35.0 40.5 24.0 41.0 56-57 34.93805676507011 39.0 35.0 41.0 24.0 41.0 58-59 34.807145817520876 39.0 35.0 41.0 23.5 41.0 60-61 34.5402044301522 38.5 34.5 40.0 23.0 41.0 62-63 34.16310803073277 38.0 34.0 40.0 22.0 41.0 64-65 33.76279228085593 38.0 34.0 40.0 20.5 41.0 66-67 33.405930421843166 37.0 33.0 40.0 19.5 41.0 68-69 33.02701184436544 37.0 33.0 39.0 18.0 41.0 70-71 32.591731830650204 36.0 32.5 39.0 13.5 40.5 72-73 32.12233060693218 36.0 32.0 39.0 10.0 40.0 74-75 31.64059732819345 35.0 31.0 38.0 7.5 40.0 76-77 31.12839260509461 35.0 31.0 37.0 4.0 39.0 78-79 30.65034779091599 35.0 31.0 37.0 2.0 39.0 80-81 30.166590933301375 35.0 30.0 36.0 2.0 38.5 82-83 29.723767959626628 34.0 30.0 36.0 2.0 37.0 84-85 29.32153266022184 34.0 29.5 35.0 2.0 37.0 86-87 28.846912076059603 34.0 29.0 35.0 2.0 36.5 88-89 28.32593559834352 34.0 29.0 35.0 2.0 36.0 90-91 27.822001624522297 34.0 27.5 35.0 2.0 36.0 92-93 27.375845134422562 34.0 26.5 35.0 2.0 35.5 94-95 26.88427775336556 34.0 25.5 35.0 2.0 35.0 96-97 26.270422209350322 33.5 25.0 35.0 2.0 35.0 98-99 25.519283296049217 33.0 21.0 35.0 2.0 35.0 100-101 23.027179123556905 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38335095868060165 1101 2 0.3690336798419622 1101 3 0.45304292702735083 1101 4 0.2646560218876104 1101 5 0.21401260633869867 1101 6 0.3746821814227559 1101 7 0.5357615463596659 1101 8 0.549654060398943 1101 9 0.6300397273904608 1101 10-11 0.4422501355465158 1101 12-13 0.5039486840164855 1101 14-15 0.5485929365311364 1101 16-17 0.7352000210201126 1101 18-19 0.7193435874913732 1101 20-21 0.7867215845456883 1101 22-23 0.7892156552701053 1101 24-25 0.5393142906519017 1101 26-27 0.6605048034000731 1101 28-29 0.737468439492794 1101 30-31 0.7480332362637157 1101 32-33 0.6226498038087485 1101 34-35 0.608147819818015 1101 36-37 0.7982335451097029 1101 38-39 0.7846546983233722 1101 40-41 0.7174134620998771 1101 42-43 0.8140856228168474 1101 44-45 1.076830656894039 1101 46-47 0.9345158771513198 1101 48-49 0.8314965978159492 1101 50-51 0.9385167396735667 1101 52-53 0.8893536754657774 1101 54-55 0.7619136626398273 1101 56-57 0.7473580007001885 1101 58-59 0.8249584479617553 1101 60-61 0.8555998951124479 1101 62-63 0.7651169233512221 1101 64-65 0.7176273731713181 1101 66-67 0.6943978985755237 1101 68-69 0.8262325207862986 1101 70-71 0.7402890519753029 1101 72-73 0.6581617992309035 1101 74-75 0.5955597458267512 1101 76-77 0.7264732381845747 1101 78-79 0.5343346124192827 1101 80-81 0.4425504716790094 1101 82-83 0.48990907827974794 1101 84-85 0.6574138841599613 1101 86-87 0.416156907971061 1101 88-89 0.5175319577950823 1101 90-91 0.6145929684982043 1101 92-93 0.5728045659304364 1101 94-95 0.6702716854172373 1101 96-97 0.5645896875722585 1101 98-99 0.5956978391346723 1101 100-101 0.8299799529073972 1102 1 0.2313124601973584 1102 2 0.13052906533773623 1102 3 0.1515388701759548 1102 4 0.11905607794485462 1102 5 0.23817488623370764 1102 6 0.28696645590473224 1102 7 0.19714802897169648 1102 8 0.20852964542348218 1102 9 0.4183973375998846 1102 10-11 0.3083036030668893 1102 12-13 0.29526426296815345 1102 14-15 0.3948544727917067 1102 16-17 0.4971599955607857 1102 18-19 0.5139663981200613 1102 20-21 0.521441604866439 1102 22-23 0.6005651313392022 1102 24-25 0.43906050021842447 1102 26-27 0.4293788702074721 1102 28-29 0.4257328399284077 1102 30-31 0.42537742192999417 1102 32-33 0.4575681412441881 1102 34-35 0.5652461418961039 1102 36-37 0.6298446508681224 1102 38-39 0.403236216365336 1102 40-41 0.5473851853055365 1102 42-43 0.7438855977078731 1102 44-45 0.8603401910057471 1102 46-47 0.8179956308497864 1102 48-49 0.6874331188213532 1102 50-51 0.7397266419529416 1102 52-53 0.6424700351314172 1102 54-55 0.48483501899166725 1102 56-57 0.719383389962303 1102 58-59 0.6095099315810657 1102 60-61 0.6788932876562512 1102 62-63 0.6405289587256817 1102 64-65 0.546915898661311 1102 66-67 0.5138428296280253 1102 68-69 0.7506800503258404 1102 70-71 0.7293144587553115 1102 72-73 0.5878395138469514 1102 74-75 0.4237245716551321 1102 76-77 0.621293271059816 1102 78-79 0.6305060045368158 1102 80-81 0.6394296595496236 1102 82-83 0.7354850811059706 1102 84-85 0.7661149472039313 1102 86-87 0.8797930526337971 1102 88-89 1.0278072952334405 1102 90-91 1.0169767153997284 1102 92-93 0.8125982051845249 1102 94-95 0.7467255973080889 1102 96-97 0.5517330160297824 1102 98-99 0.5711614792938526 1102 100-101 0.4976290812463766 1103 1 0.19101359897675607 1103 2 0.3268600297084916 1103 3 0.3515658537415085 1103 4 0.279619961536298 1103 5 0.37574512554876804 1103 6 0.2924608932791628 1103 7 0.38564282004961825 1103 8 0.2879503009782667 1103 9 0.3016737258861042 1103 10-11 0.4785224981263383 1103 12-13 0.401596649445743 1103 14-15 0.35338328504077765 1103 16-17 0.41845039342251766 1103 18-19 0.5107447791790563 1103 20-21 0.5781224075181157 1103 22-23 0.5351026366721001 1103 24-25 0.6850390373445308 1103 26-27 0.39896401611927246 1103 28-29 0.29960691419155694 1103 30-31 0.4399602158958871 1103 32-33 0.39166260560217836 1103 34-35 0.306563450394826 1103 36-37 0.4065403309451341 1103 38-39 0.5038736119407687 1103 40-41 0.54579753065142 1103 42-43 0.5101816657647973 1103 44-45 0.5917326523809621 1103 46-47 0.5635588398520852 1103 48-49 0.652720584955155 1103 50-51 0.6378250298672228 1103 52-53 0.6105433720868731 1103 54-55 0.5750122718459068 1103 56-57 0.6669072831803078 1103 58-59 0.7368380845560054 1103 60-61 0.7148944690988444 1103 62-63 0.7707531046411376 1103 64-65 0.6270742265553864 1103 66-67 0.6045984059277032 1103 68-69 0.6636755542803954 1103 70-71 0.6833450376986505 1103 72-73 0.9021189250218029 1103 74-75 0.6489932222216019 1103 76-77 0.6454698165070702 1103 78-79 0.6852552943950094 1103 80-81 0.7720801385079135 1103 82-83 0.6897468435703686 1103 84-85 0.7716061027933598 1103 86-87 0.5841793406067843 1103 88-89 0.649000698116307 1103 90-91 0.9142367895680685 1103 92-93 0.7771450818368102 1103 94-95 0.7277484932280984 1103 96-97 0.7999391558848714 1103 98-99 1.1029029038073261 1103 100-101 1.4262706775066043 1104 1 0.09904744241832475 1104 2 0.19632723393964113 1104 3 0.13713470390263183 1104 4 0.13367264863619255 1104 5 0.1652257688920713 1104 6 0.21987812713415877 1104 7 0.23330398101550287 1104 8 0.2051783757170753 1104 9 0.3471159882811534 1104 10-11 0.28036062185287847 1104 12-13 0.3665220117960004 1104 14-15 0.3587260546539426 1104 16-17 0.3599310078645104 1104 18-19 0.45678240869307984 1104 20-21 0.40420223295416235 1104 22-23 0.32961969567414684 1104 24-25 0.3557822346971662 1104 26-27 0.2306391364210043 1104 28-29 0.28241099216730703 1104 30-31 0.33454699932386944 1104 32-33 0.3659982365901655 1104 34-35 0.4673237717774654 1104 36-37 0.4655380332904784 1104 38-39 0.6145038773993576 1104 40-41 0.5828331226226311 1104 42-43 0.4238039400093925 1104 44-45 0.4991032106098814 1104 46-47 0.4711667501423946 1104 48-49 0.5027278102842772 1104 50-51 0.513146195746323 1104 52-53 0.4326734713628895 1104 54-55 0.5255812665136119 1104 56-57 0.3997973425725192 1104 58-59 0.4833377738668503 1104 60-61 0.5078526633446003 1104 62-63 0.29381021364245186 1104 64-65 0.524558366957983 1104 66-67 0.3654468763255281 1104 68-69 0.45367967271798904 1104 70-71 0.3731555173160004 1104 72-73 0.4225062749155839 1104 74-75 0.3552398805068826 1104 76-77 0.4673354687375273 1104 78-79 0.1969964542048359 1104 80-81 -0.11372100598422108 1104 82-83 0.021865237813226912 1104 84-85 0.14631492203007568 1104 86-87 0.25530439660308346 1104 88-89 0.27583665424410597 1104 90-91 0.2387859290313692 1104 92-93 0.20273792009059477 1104 94-95 0.3731770274978352 1104 96-97 0.6002556253000542 1104 98-99 0.6436385868176906 1104 100-101 1.1585057608146485 1105 1 0.1774365382492782 1105 2 0.257495393945014 1105 3 0.14089042277462838 1105 4 0.14947295513417203 1105 5 0.20382605749235694 1105 6 0.24551936789168138 1105 7 0.14329257123664974 1105 8 0.2121165280040671 1105 9 0.17002671235466948 1105 10-11 0.3108482221079214 1105 12-13 0.22852940578013659 1105 14-15 0.36896689047617315 1105 16-17 0.36307988310640127 1105 18-19 0.3308847275628395 1105 20-21 0.22590626105354517 1105 22-23 0.16656892215825536 1105 24-25 0.2758368785890184 1105 26-27 0.33965705648543576 1105 28-29 0.26791106487667804 1105 30-31 0.2989445741865637 1105 32-33 0.354374524385058 1105 34-35 0.31177604646230606 1105 36-37 0.37680183408915724 1105 38-39 0.5218651423187595 1105 40-41 0.5560327266361824 1105 42-43 0.34695572362628724 1105 44-45 0.6033530735806849 1105 46-47 0.47928503151649693 1105 48-49 0.7131725120080432 1105 50-51 0.7514455793945487 1105 52-53 0.6887223320629161 1105 54-55 0.5703814275928494 1105 56-57 0.4732687677881273 1105 58-59 0.5281071732874167 1105 60-61 0.5222337387721865 1105 62-63 0.5675638071634879 1105 64-65 0.43542086793675594 1105 66-67 0.2623743459274195 1105 68-69 0.48889478060750946 1105 70-71 0.372273217015092 1105 72-73 0.3432964021010676 1105 74-75 0.23841688868389355 1105 76-77 0.40690558854020864 1105 78-79 0.5155669878916491 1105 80-81 0.4201937226979524 1105 82-83 0.2506564724184166 1105 84-85 0.3379432764491277 1105 86-87 0.256144749187623 1105 88-89 0.21183702338401034 1105 90-91 0.4463222557537456 1105 92-93 0.47709695898451443 1105 94-95 0.5983607584955202 1105 96-97 0.5365873813992508 1105 98-99 0.7566247608503751 1105 100-101 0.8541890611491105 1106 1 -0.06839992950124696 1106 2 0.19080057329614064 1106 3 0.22661193661112833 1106 4 0.17951517272206985 1106 5 0.12232496390424075 1106 6 0.13160349252592596 1106 7 0.06968552408520168 1106 8 0.16920624506323634 1106 9 0.20371243895495894 1106 10-11 0.29947196086752115 1106 12-13 0.2892111649444544 1106 14-15 0.3811620042655548 1106 16-17 0.40198056603009746 1106 18-19 0.30930644030655685 1106 20-21 0.37303167700520135 1106 22-23 0.3571380296993212 1106 24-25 0.2055015606453381 1106 26-27 0.22777609830414036 1106 28-29 0.23596309932537451 1106 30-31 0.05448747309190338 1106 32-33 0.14222545641126771 1106 34-35 0.25510522585626205 1106 36-37 0.34832236329479116 1106 38-39 0.3793627128665733 1106 40-41 0.5628549362463744 1106 42-43 0.4791102260771609 1106 44-45 0.6693234088316942 1106 46-47 0.7284715331321152 1106 48-49 0.7355238067788932 1106 50-51 0.7410551537116916 1106 52-53 0.5338514477423146 1106 54-55 0.3441140968312979 1106 56-57 0.5754550070496833 1106 58-59 0.6272652978306397 1106 60-61 0.7476383797620585 1106 62-63 0.7962157489851336 1106 64-65 0.6805040716546955 1106 66-67 0.7274651927153215 1106 68-69 0.675811343983419 1106 70-71 0.5947106469090002 1106 72-73 0.5583825639949396 1106 74-75 0.5063171994931501 1106 76-77 0.6769873867899285 1106 78-79 0.742908163291407 1106 80-81 0.7148782419341266 1106 82-83 0.6385365846417059 1106 84-85 0.7743461746584224 1106 86-87 0.8778429028039589 1106 88-89 1.1052864707003671 1106 90-91 1.0409015656554708 1106 92-93 1.04489641071806 1106 94-95 1.2669332846706922 1106 96-97 1.0807251065753363 1106 98-99 1.1201380699650372 1106 100-101 1.5892542882593865 1107 1 -0.11171391262874408 1107 2 -0.07102382769713245 1107 3 -0.07142092036727732 1107 4 0.009482447303135189 1107 5 -0.06882700373213169 1107 6 -0.08087360790328546 1107 7 0.022139272707931923 1107 8 -0.025021583054311236 1107 9 -0.07145406600318438 1107 10-11 -0.04371276507765742 1107 12-13 0.0442365723677014 1107 14-15 0.19873670537887733 1107 16-17 0.044741012674514025 1107 18-19 0.03969108731604365 1107 20-21 0.016435449473092945 1107 22-23 0.09530409064424816 1107 24-25 -0.1966619348898675 1107 26-27 -0.19900217464888215 1107 28-29 -0.06018812828895648 1107 30-31 -0.14030079838106957 1107 32-33 -0.3109054945992753 1107 34-35 -0.23927521125008155 1107 36-37 -0.07415687497586276 1107 38-39 -0.2710252231297474 1107 40-41 -0.08412626719390204 1107 42-43 -0.11933046526787194 1107 44-45 -0.008058886552213096 1107 46-47 0.049975586808379546 1107 48-49 0.08846202316134111 1107 50-51 0.07022583568311092 1107 52-53 0.09535023903965367 1107 54-55 -0.015757632682422695 1107 56-57 0.06553739616971654 1107 58-59 0.0802452843457857 1107 60-61 0.10847246682154577 1107 62-63 0.08351302405954897 1107 64-65 0.15912993295811617 1107 66-67 -0.32976613817319844 1107 68-69 -0.06283754022779675 1107 70-71 -0.08234472318424224 1107 72-73 -0.09382747070387154 1107 74-75 -0.02750147866304431 1107 76-77 -0.06332934264156975 1107 78-79 0.061010746790557846 1107 80-81 0.029102123267481517 1107 82-83 0.11233421328220317 1107 84-85 0.10522614308415257 1107 86-87 0.2267807245079858 1107 88-89 0.3157572891647398 1107 90-91 0.5037261475430199 1107 92-93 0.4601178417562295 1107 94-95 0.3385078871144067 1107 96-97 0.3981488431932725 1107 98-99 0.41490430522626554 1107 100-101 0.9882659477176929 1108 1 0.029687472081445065 1108 2 0.11417354787831968 1108 3 0.03598256678246514 1108 4 -0.04746974032146767 1108 5 0.09598540791103716 1108 6 0.12151737335599933 1108 7 -0.0905652620366908 1108 8 -0.07412442134771169 1108 9 -0.14459358824192492 1108 10-11 -0.07531424403512688 1108 12-13 -0.014146724112528375 1108 14-15 -0.054931689154784635 1108 16-17 0.15127996217970718 1108 18-19 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-2.9941891674708323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6551.0 3 1260.0 4 993.0 5 1154.0 6 1282.0 7 1556.0 8 1977.0 9 2301.0 10 2901.0 11 3695.0 12 5021.0 13 6446.0 14 6863.0 15 8029.0 16 9920.0 17 12614.0 18 11291.0 19 7707.0 20 7399.0 21 7941.0 22 8532.0 23 9589.0 24 11004.0 25 12467.0 26 14720.0 27 16935.0 28 20121.0 29 23669.0 30 27963.0 31 34450.0 32 42314.0 33 53062.0 34 70216.0 35 100875.0 36 138109.0 37 222090.0 38 243645.0 39 44914.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.52766558291997 21.80408775010181 15.45565762505925 21.212589041918967 2 13.899823763720004 23.15422925388862 41.44286993362378 21.503077048767597 3 18.03629741061571 29.15617359515145 31.99822548957475 20.80930350465809 4 11.294379111338229 18.971739251854192 41.18778338066939 28.546098256138198 5 11.314675758806898 39.81576569774863 36.49105565988232 12.378502883562152 6 25.944555816102227 40.274835972341286 19.57040931029997 14.210198901256515 7 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33.37233742785878 19.282647491624836 30-31 19.05460257733981 30.179115017065133 32.30994017813843 18.456342227456624 32-33 18.719621562530488 30.174893078740244 31.553925087992717 19.551560270736555 34-35 19.30654407164592 29.095421814351734 31.681619350145045 19.916414763857297 36-37 20.241408612401813 28.78166023149893 31.848983421749 19.127947734350258 38-39 20.417190706502467 28.455457066465723 32.327204695285545 18.800147531746266 40-41 19.017382819105695 28.91566466286784 33.36669518499543 18.700257333031033 42-43 18.63728007357926 29.94207137137852 32.01758617765865 19.403062377383566 44-45 18.848521835678202 30.30064550247311 31.056133610198046 19.794699051650642 46-47 19.093745953104047 29.638150062035002 30.099172448941225 21.16893153591973 48-49 19.43409510160508 29.428600676461926 30.705647140914248 20.431657081018745 50-51 20.341756788656014 29.66482349181016 30.630098411750083 19.36332130778374 52-53 19.063051485333833 29.84814099307468 31.709153319607474 19.37965420198401 54-55 18.684446457173845 29.578711041779638 31.641665838086872 20.095176662959645 56-57 19.506596190352877 30.651014854741803 29.91276781034047 19.92962114456485 58-59 18.917610901976996 29.536891466701743 30.867903098177507 20.677594533143758 60-61 19.30865376667983 29.18272068553548 30.892051037795788 20.616574509988904 62-63 20.689882755520948 28.724688509305874 30.803158934189707 19.78226980098347 64-65 20.17335550246812 28.758100836691224 31.3291410323858 19.739402628454854 66-67 19.018830487998024 29.211264109493555 31.236604051599237 20.53330135090918 68-69 18.820854760148524 30.191509234861574 30.96022082592406 20.027415179065837 70-71 19.187126755736834 29.77349375436192 30.204649646658616 20.834729843242634 72-73 19.12442328915209 29.14520389269637 29.90476357623615 21.82560924191539 74-75 19.46652649295656 29.535049207741366 30.18488472230521 20.81353957699686 76-77 20.177899101104444 28.631427754966985 30.80847959812901 20.382193545799556 78-79 19.353449608096852 28.610809225908795 31.209365578849678 20.826375587144675 80-81 19.12077501324159 30.23038698865317 30.291540586105437 20.357297411999806 82-83 19.40593306616391 29.783890210160934 29.853739153465895 20.956437570209257 84-85 19.54916901065416 28.786909708891585 29.832482005988187 21.83143927446607 86-87 20.03732082120845 28.942184057567804 30.383050147921452 20.637444973302287 88-89 20.88697046453693 27.780221763504905 30.712921080985332 20.619886690972837 90-91 19.86409242918277 28.452586752647303 31.229197980691964 20.45412283747796 92-93 19.728572246391014 29.356702518006806 30.430069829256585 20.4846554063456 94-95 19.629101367341846 29.27266641063049 29.778330897646406 21.31990132438126 96-97 20.041422385890982 28.43426139481276 29.30057097867073 22.22374524062553 98-99 20.49752981894254 28.261069428343855 29.41487925741618 21.82652149529742 100-101 21.321504114477605 27.980077046449708 29.830871303351703 20.867547535720984 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6446.0 1 3810.5 2 1334.5 3 1383.0 4 1117.5 5 1128.0 6 1738.0 7 3107.5 8 4396.5 9 4506.5 10 3374.0 11 2395.5 12 2289.5 13 2252.0 14 2219.5 15 2166.0 16 2130.0 17 2201.5 18 2283.0 19 2457.5 20 2770.0 21 3206.5 22 3763.5 23 4348.5 24 4955.0 25 6090.0 26 8268.5 27 10598.5 28 11983.5 29 14346.0 30 18441.5 31 21852.5 32 26647.0 33 33683.0 34 40627.0 35 45282.0 36 50297.0 37 55685.5 38 56156.0 39 54634.5 40 51921.0 41 48579.0 42 45657.0 43 44000.5 44 43685.0 45 42581.5 46 41231.0 47 42520.5 48 71936.0 49 70794.0 50 36307.5 51 26187.5 52 20146.5 53 16861.5 54 14247.0 55 12139.0 56 10490.0 57 8689.5 58 7168.0 59 5843.0 60 4467.0 61 3354.5 62 2433.5 63 1759.5 64 1271.0 65 934.5 66 695.0 67 541.5 68 418.5 69 290.5 70 230.0 71 194.0 72 168.0 73 147.5 74 132.0 75 118.0 76 89.0 77 56.5 78 39.0 79 29.5 80 21.5 81 16.0 82 8.0 83 8.0 84 9.0 85 5.5 86 2.0 87 2.0 88 2.0 89 1.0 90 2.0 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27230722113476874 2 0.17127391842767548 3 0.19807187845377436 4 0.16686307407555362 5 0.24076552284318034 6 0.1994034541072451 7 0.24317900371509607 8 0.24808918893726947 9 0.14971903753711766 10-11 0.20760096672392442 12-13 0.2139259510779105 14-15 0.17618410364984885 16-17 0.2897841515865724 18-19 0.23510632631592965 20-21 0.19969473628144185 22-23 0.2467992250229697 24-25 0.23976684110307728 26-27 0.35012117338446586 28-29 0.32011910944220295 30-31 0.2935292081119589 32-33 0.1944932688850717 34-35 0.36310403600580565 36-37 0.3973921090826775 38-39 0.3789581086299418 40-41 0.3419236607677865 42-43 0.3751298286262134 44-45 0.38361862341708947 46-47 0.389402655161853 48-49 0.4091682312680595 50-51 0.4016781182172865 52-53 0.40080427169469635 54-55 0.3958940864725229 56-57 0.37379825297274266 58-59 0.34841509207845645 60-61 0.3166653350910132 62-63 0.3826615534161573 64-65 0.41823959040732894 66-67 0.4238987869345797 68-69 0.42997410085354 70-71 0.4285176899825564 72-73 0.4190718251907482 74-75 0.4139119695335491 76-77 0.39797467343107096 78-79 0.3725915125367848 80-81 0.3826615534161573 82-83 0.3924403121213332 84-85 0.3934389938614362 86-87 0.3882375264650661 88-89 0.396851156473455 90-91 0.4190718251907482 92-93 0.4179899199723032 94-95 0.42764384345996614 96-97 0.4268948321548889 98-99 0.42552164476224713 100-101 0.3972672738651647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1201584.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.01252639487555 #Duplication Level Percentage of deduplicated Percentage of total 1 70.96903992744144 23.428673038236376 2 12.369997433208885 8.167297335367024 3 5.3096571240362405 5.258555878649562 4 2.9814062496186216 3.936950100375267 5 1.75515747884673 2.897109129879545 6 1.218516262338427 2.4135780163819454 7 0.8836920424564418 2.042103481357434 8 0.6246104267429956 1.64959745594941 9 0.46464778811095514 1.3805277632400101 >10 2.878190034195559 17.76643343272858 >50 0.3133261049004216 7.24890280242297 >100 0.21706838557855068 13.105708489912377 >500 0.007598659926596101 1.6738448483846005 >1k 0.005319061948617271 4.262125068031764 >5k 0.0012664433210993501 2.7833586506233488 >10k+ 5.065773284397401E-4 1.9852345084597454 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13247 1.1024614175954408 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10495 0.8734304051984714 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8176 0.6804351589235542 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8130 0.6766068789198258 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6483 0.5395378100906802 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5414 0.45057191174316563 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5084 0.4231081638903314 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4716 0.3924819238605042 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4632 0.38549115167978265 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4512 0.37550433427875207 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3926 0.32673537597038577 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3293 0.27405491417994915 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3161 0.2630694150388154 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2742 0.2281987776135501 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2647 0.22029254717106755 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2292 0.19074821235968523 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2270 0.18891729583616293 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2064 0.171773259297727 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1883 0.15670980971783913 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1867 0.15537823406436838 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1528 0.1271654749064568 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1470 0.12233851316262533 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1448 0.12050759663910304 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1385 0.11526451750356195 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1375 0.11443228272014275 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1352 0.11251814271827854 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1220 0.10153264357714484 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.3223478341922E-5 0.0 10-11 0.0 0.0 0.0 2.4967043502576597E-4 0.0 12-13 0.0 0.0 0.0 4.1611739170961E-4 0.0 14-15 0.0 0.0 0.0 5.409526092224929E-4 0.0 16-17 0.0 0.0 0.0 7.49011305077298E-4 0.0 18-19 0.0 0.0 0.0 9.986817401030639E-4 0.0 20-21 0.0 0.0 0.0 0.00124835217512883 0.0 22-23 0.0 0.0 0.0 0.0020805869585480497 0.0 24-25 0.0 0.0 0.0 0.004535679569634749 0.0 26-27 0.0 0.0 0.0 0.008197512616679316 0.0 28-29 0.0 0.0 0.0 0.018101106539368035 0.0 30-31 0.0 0.0 0.0 0.04631386569727959 0.0 32-33 0.0 0.0 0.0 0.09337674269963647 0.0 34-35 0.0 0.0 0.0 0.17023362494840144 0.0 36-37 0.0 0.0 0.0 0.2769677359219164 0.0 38-39 0.0 0.0 0.0 0.42814318433001775 0.0 40-41 4.1611739170961E-5 0.0 0.0 0.5951727053622552 0.0 42-43 8.3223478341922E-5 0.0 0.0 0.7602464746534574 0.0 44-45 8.3223478341922E-5 0.0 0.0 0.9557384252786323 0.0 46-47 8.3223478341922E-5 0.0 0.0 1.201455745083157 0.0 48-49 8.3223478341922E-5 0.0 0.0 1.4695601805616585 0.0 50-51 8.3223478341922E-5 0.0 0.0 1.7415345077830597 0.0 52-53 8.3223478341922E-5 4.1611739170961E-5 0.0 1.9921204010705869 0.0 54-55 8.3223478341922E-5 8.3223478341922E-5 0.0 2.266050480033023 0.0 56-57 8.3223478341922E-5 8.3223478341922E-5 0.0 2.5855454133876616 0.0 58-59 8.3223478341922E-5 8.3223478341922E-5 0.0 2.9586778785336687 0.0 60-61 8.3223478341922E-5 8.3223478341922E-5 0.0 3.4003032663550776 0.0 62-63 8.3223478341922E-5 8.3223478341922E-5 0.0 3.8137575067577467 0.0 64-65 8.3223478341922E-5 8.3223478341922E-5 0.0 4.2073629475758665 0.0 66-67 8.3223478341922E-5 8.3223478341922E-5 0.0 4.653690461923594 0.0 68-69 8.3223478341922E-5 8.3223478341922E-5 0.0 5.153239390670981 0.0 70-71 8.3223478341922E-5 8.3223478341922E-5 0.0 5.7076325916456945 0.0 72-73 8.3223478341922E-5 8.3223478341922E-5 0.0 6.289697599169097 0.0 74-75 8.3223478341922E-5 8.3223478341922E-5 0.0 6.875923780609595 0.0 76-77 8.3223478341922E-5 8.3223478341922E-5 0.0 7.464230549008642 0.0 78-79 8.3223478341922E-5 8.3223478341922E-5 0.0 8.131100280962464 0.0 80-81 8.3223478341922E-5 8.3223478341922E-5 0.0 8.847904099921436 0.0 82-83 8.3223478341922E-5 8.3223478341922E-5 0.0 9.563501178444454 0.0 84-85 8.3223478341922E-5 8.3223478341922E-5 0.0 10.34834019094795 0.0 86-87 8.3223478341922E-5 8.3223478341922E-5 0.0 11.181157538715562 0.0 88-89 8.3223478341922E-5 8.3223478341922E-5 0.0 11.963832740782168 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 45 9.6817384E-4 42.17204 4 TAGGACG 70 2.0677787E-4 33.888245 4 GTACACG 60 0.0039312467 31.699133 1 CTTTGCG 230 0.0 30.94144 2 TATAACG 65 0.0058782296 29.196028 2 GGACGTG 100 4.9936003E-5 28.466124 6 AATCGAG 35 0.008424158 27.122478 18-19 CCGTATC 45 8.9361746E-4 26.396072 94-95 TTACCGG 45 8.951447E-4 26.388355 78-79 GACTGCG 145 2.3552491E-5 22.904736 7 GAGTACT 5810 0.0 22.87438 12-13 CCGGATA 55 0.002878605 21.590471 82-83 TTGCGTT 330 1.8189894E-12 21.565247 4 GTATAGG 600 0.0 21.396914 1 CATATAG 495 0.0 21.08602 3 GTACTTT 6270 0.0 20.893394 14-15 ACTGCGA 160 5.0338065E-5 20.757416 8 GCTTTGC 415 0.0 20.623533 1 ATGGGAT 1105 0.0 20.60896 5 AGTACTT 6160 0.0 20.303333 12-13 >>END_MODULE