##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938722_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1201584 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02458754444134 33.0 31.0 34.0 30.0 34.0 2 32.22951953421484 34.0 31.0 34.0 30.0 34.0 3 32.29952962090041 34.0 31.0 34.0 30.0 34.0 4 35.9192499234344 37.0 35.0 37.0 35.0 37.0 5 35.82782227459753 37.0 35.0 37.0 35.0 37.0 6 35.86100597211681 37.0 35.0 37.0 35.0 37.0 7 35.791960445545214 37.0 35.0 37.0 33.0 37.0 8 35.789433780742755 37.0 35.0 37.0 33.0 37.0 9 37.419082644242934 39.0 37.0 39.0 34.0 39.0 10-11 37.35387496837508 39.0 37.0 39.0 34.0 39.0 12-13 37.290922232652896 39.0 37.0 39.0 33.5 39.0 14-15 38.44041282174197 40.0 38.0 41.0 33.5 41.0 16-17 38.366993485266114 40.0 38.0 41.0 33.0 41.0 18-19 38.56807846975326 40.0 38.0 41.0 34.0 41.0 20-21 38.588453657838315 40.0 38.0 41.0 34.0 41.0 22-23 38.51501684443201 40.0 38.0 41.0 34.0 41.0 24-25 38.434191034501126 40.0 38.0 41.0 34.0 41.0 26-27 38.208265922315874 40.0 37.5 41.0 33.5 41.0 28-29 38.03638072743978 40.0 37.0 41.0 33.0 41.0 30-31 37.83252232053689 40.0 37.0 41.0 33.0 41.0 32-33 37.66496724323892 40.0 36.5 41.0 33.0 41.0 34-35 37.631155624575555 40.0 37.0 41.0 33.0 41.0 36-37 37.48156350284292 40.0 36.0 41.0 32.0 41.0 38-39 37.32939727892515 40.0 36.0 41.0 32.0 41.0 40-41 37.149955808333004 40.0 36.0 41.0 31.0 41.0 42-43 36.99271503282334 40.0 36.0 41.0 31.0 41.0 44-45 36.77034397928068 40.0 36.0 41.0 30.5 41.0 46-47 36.45701299284944 40.0 35.0 41.0 30.0 41.0 48-49 36.167448551245684 39.0 35.0 41.0 29.5 41.0 50-51 35.974958471484314 39.0 35.0 41.0 29.0 41.0 52-53 35.739561695228964 39.0 35.0 41.0 28.0 41.0 54-55 35.52734973168751 39.0 35.0 40.0 27.5 41.0 56-57 35.34473286927921 39.0 35.0 40.0 27.0 41.0 58-59 35.11494868440326 38.0 35.0 40.0 26.5 41.0 60-61 34.7793828812634 38.0 34.0 40.0 26.0 41.0 62-63 34.41497764617372 38.0 34.0 40.0 25.5 41.0 64-65 33.98121854152519 37.5 33.5 40.0 23.5 41.0 66-67 33.598123393786864 37.0 33.0 39.5 23.0 41.0 68-69 33.306842884059705 36.5 33.0 39.0 22.5 40.0 70-71 32.882031967802504 36.0 32.0 39.0 22.0 40.0 72-73 32.36581504081279 35.5 32.0 38.0 20.5 40.0 74-75 31.8498082531059 35.0 31.0 37.5 18.5 39.5 76-77 29.79963156966138 33.0 28.5 35.5 13.0 38.5 78-79 30.734213754510712 34.0 30.5 36.0 13.0 39.0 80-81 30.78854412175928 34.5 31.0 36.0 13.0 38.5 82-83 30.443753828280002 34.0 31.0 36.0 7.5 37.0 84-85 30.01123225675442 34.0 30.0 35.0 4.5 37.0 86-87 29.40087542776868 34.0 29.5 35.0 2.0 36.0 88-89 28.749104931490432 34.0 29.0 35.0 2.0 36.0 90-91 28.188796205675175 33.5 28.0 35.0 2.0 35.5 92-93 27.920472892448636 33.0 28.0 35.0 2.0 35.0 94-95 27.604858669889083 33.0 27.0 35.0 2.0 35.0 96-97 26.96738263825084 33.0 26.0 35.0 2.0 35.0 98-99 26.145734297394107 33.0 25.0 35.0 2.0 35.0 100-101 22.47485277766681 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09981853884846714 1101 2 0.17920184179340026 1101 3 0.17988607909004628 1101 4 0.19336951705634675 1101 5 0.07043155056722128 1101 6 0.15702441297830205 1101 7 0.13309266529412866 1101 8 0.1614331578319863 1101 9 0.26553055067503806 1101 10-11 0.19104235321122331 1101 12-13 0.248561051474546 1101 14-15 0.4257997965927629 1101 16-17 0.41706174734502355 1101 18-19 0.3746100945131161 1101 20-21 0.48803009752765547 1101 22-23 0.3250631420973349 1101 24-25 0.2757042687936391 1101 26-27 0.2703474901366647 1101 28-29 0.4956157640319887 1101 30-31 0.4283811500494963 1101 32-33 0.3970653959270365 1101 34-35 0.520493878761144 1101 36-37 0.42417345740044965 1101 38-39 0.5422286299060914 1101 40-41 0.511991582627978 1101 42-43 0.6675580295877666 1101 44-45 0.7580963059161903 1101 46-47 0.6943592194760626 1101 48-49 0.5430232251377092 1101 50-51 0.4450446333597071 1101 52-53 0.3361978940371415 1101 54-55 0.49012090719332235 1101 56-57 0.5543203010045801 1101 58-59 0.49784830054672824 1101 60-61 0.6525796062969746 1101 62-63 0.33162494574119705 1101 64-65 0.37901559300748744 1101 66-67 0.5728465900487194 1101 68-69 0.5967638416824244 1101 70-71 0.727881997365742 1101 72-73 0.6270944246271668 1101 74-75 0.6147175498450252 1101 76-77 0.507563329437744 1101 78-79 0.30344793840770734 1101 80-81 0.48857345079263226 1101 82-83 0.5564467624291645 1101 84-85 0.6938546787153363 1101 86-87 0.8260928137366683 1101 88-89 0.8479727562250403 1101 90-91 0.830802454682182 1101 92-93 0.7412785655888499 1101 94-95 0.7970754510321676 1101 96-97 0.8251565623015686 1101 98-99 1.03688676719905 1101 100-101 1.7743976564012875 1102 1 0.19012545228380873 1102 2 0.2111143282535295 1102 3 0.18721220673123184 1102 4 0.04332951787385042 1102 5 -0.14948220302029824 1102 6 -0.12791063944398928 1102 7 -0.060041343645835354 1102 8 0.02840375989267585 1102 9 0.030014196264765758 1102 10-11 0.13218406710320352 1102 12-13 0.14599015511330293 1102 14-15 0.12965767395001393 1102 16-17 0.16555739852879015 1102 18-19 0.23823441460000083 1102 20-21 0.17095701580961986 1102 22-23 0.0440787388588646 1102 24-25 0.1402293003988362 1102 26-27 0.15422760061616003 1102 28-29 0.11682898011223841 1102 30-31 0.10450492270231848 1102 32-33 0.22146314546208856 1102 34-35 0.3730600306938072 1102 36-37 0.2816676108046252 1102 38-39 0.31743066957243116 1102 40-41 0.1877803256317705 1102 42-43 0.22080475155257062 1102 44-45 0.2037707738832637 1102 46-47 0.37445042929075356 1102 48-49 0.6064252140021651 1102 50-51 0.4810249198940042 1102 52-53 0.2392422113795618 1102 54-55 0.13691024288572606 1102 56-57 0.2535410468579542 1102 58-59 0.49205827289765836 1102 60-61 0.27969927100456005 1102 62-63 0.24453803948818376 1102 64-65 0.4629846797706776 1102 66-67 0.5125792086521344 1102 68-69 0.5053273892887518 1102 70-71 0.3685630637381152 1102 72-73 0.3697077142825691 1102 74-75 0.21992525362918514 1102 76-77 0.21479084595565467 1102 78-79 0.15438054708431892 1102 80-81 0.350825171195428 1102 82-83 0.42717203227766376 1102 84-85 0.5821595162807007 1102 86-87 0.5216983050113022 1102 88-89 0.4617390442133136 1102 90-91 0.5775382878184381 1102 92-93 0.5994668653900206 1102 94-95 0.5440360970919578 1102 96-97 0.4860229825499154 1102 98-99 0.742364684438698 1102 100-101 1.2729608244533246 1103 1 0.1512078579051419 1103 2 0.09128906507260126 1103 3 0.13826328953796008 1103 4 -0.021328210182858243 1103 5 0.031881633896865935 1103 6 -0.01295700377317388 1103 7 0.034425202202527316 1103 8 0.08163285401062126 1103 9 0.0400302040827043 1103 10-11 0.163952475182235 1103 12-13 0.1283414446724933 1103 14-15 0.12975758607110777 1103 16-17 0.041259791679337354 1103 18-19 -0.08630203326884356 1103 20-21 0.021427587314242658 1103 22-23 0.15179020432172052 1103 24-25 0.051045699113835497 1103 26-27 0.12784678957769557 1103 28-29 -0.06495419599928454 1103 30-31 0.03672038282680745 1103 32-33 0.1240258549037847 1103 34-35 -0.07709804195032177 1103 36-37 0.07572648602751286 1103 38-39 0.04661376035754472 1103 40-41 0.08821497982279425 1103 42-43 0.02491500494283372 1103 44-45 0.1769512975832228 1103 46-47 0.33800183115990734 1103 48-49 0.2784556543812542 1103 50-51 0.35497192271268574 1103 52-53 0.2966391724466604 1103 54-55 0.27336759244323616 1103 56-57 0.07284013051064875 1103 58-59 0.3891447771038159 1103 60-61 0.5118480015007307 1103 62-63 0.4820375381043718 1103 64-65 0.5010249436261844 1103 66-67 0.5177502078827416 1103 68-69 0.5268978328037619 1103 70-71 0.4574020775981822 1103 72-73 0.33396506456936237 1103 74-75 0.47525432550392566 1103 76-77 0.42016859764121506 1103 78-79 0.3506283729017561 1103 80-81 0.43769000905701816 1103 82-83 0.6190973826082136 1103 84-85 0.7305687621629708 1103 86-87 0.8756621543263172 1103 88-89 0.5083823251231685 1103 90-91 0.8071582206512424 1103 92-93 0.7926214265242066 1103 94-95 0.8559511644961759 1103 96-97 0.9639376634327874 1103 98-99 1.247870425739233 1103 100-101 2.0170723905575905 1104 1 0.05423382573798108 1104 2 -0.031270580019231886 1104 3 0.013410324985429156 1104 4 0.025904488766578027 1104 5 -0.23416076433436928 1104 6 -0.06240354356305744 1104 7 -0.10827288019513048 1104 8 -0.05760429574154813 1104 9 -0.02991531693577798 1104 10-11 0.008859701956410504 1104 12-13 -0.12798794363114752 1104 14-15 -0.10310610086983729 1104 16-17 -0.005011215965062377 1104 18-19 0.04118998274248753 1104 20-21 0.08465788745441216 1104 22-23 0.021854943270582794 1104 24-25 0.04510358845069362 1104 26-27 -0.03505620315362279 1104 28-29 -0.027983631990089464 1104 30-31 -0.2642409472117748 1104 32-33 -0.24197969414898068 1104 34-35 -0.09623179724287922 1104 36-37 -0.05105997659586592 1104 38-39 -0.054481599536096326 1104 40-41 -0.056858429521000176 1104 42-43 -0.08648150666826382 1104 44-45 0.0549696788204912 1104 46-47 0.03168869984677514 1104 48-49 0.03266187972482015 1104 50-51 0.025488244731157295 1104 52-53 0.2561794970084392 1104 54-55 0.3437213445566485 1104 56-57 0.1724300930834417 1104 58-59 0.38770476901144946 1104 60-61 0.5533744967825882 1104 62-63 0.2558977424366802 1104 64-65 0.31241748130627656 1104 66-67 0.2546691107887682 1104 68-69 0.30850322867954105 1104 70-71 0.363304087684746 1104 72-73 0.43484113572440464 1104 74-75 0.4733945257900416 1104 76-77 0.436808530590163 1104 78-79 0.42059015091074414 1104 80-81 0.6205591149947942 1104 82-83 0.5292677558687124 1104 84-85 0.7511154305637184 1104 86-87 0.7507214020250537 1104 88-89 0.7095481319756729 1104 90-91 0.5436873170515817 1104 92-93 0.4660650906331085 1104 94-95 0.35276985208739475 1104 96-97 0.36751311754472127 1104 98-99 0.6253573840201803 1104 100-101 1.3856649686995262 1105 1 0.0647207548295583 1105 2 0.0465312477825961 1105 3 0.11043259642629977 1105 4 -0.0017418528332484584 1105 5 -0.06198216773717036 1105 6 -0.024150022751399547 1105 7 -0.030448680277906703 1105 8 0.10603933883231775 1105 9 0.08835766761627895 1105 10-11 0.054922489298270705 1105 12-13 0.1594965357137994 1105 14-15 0.2949301867013361 1105 16-17 0.20620532373985156 1105 18-19 0.21139983318489897 1105 20-21 0.011969486277642716 1105 22-23 0.006110567061092809 1105 24-25 0.09082939580441263 1105 26-27 0.12247984391731137 1105 28-29 0.04955252694141876 1105 30-31 0.18179331568295254 1105 32-33 0.27568448921287825 1105 34-35 0.3636969475695935 1105 36-37 0.3368571235305424 1105 38-39 0.2376996257846642 1105 40-41 0.3060200787063465 1105 42-43 0.3866901322010392 1105 44-45 0.1776499701054277 1105 46-47 0.0579353859771814 1105 48-49 0.2188132606436426 1105 50-51 0.3010344039982513 1105 52-53 0.27063607832548087 1105 54-55 0.12076503729800692 1105 56-57 0.16812765564147014 1105 58-59 0.30508734337076504 1105 60-61 0.29660553361827624 1105 62-63 0.18106581295358382 1105 64-65 0.279875841206497 1105 66-67 0.19868429026906398 1105 68-69 0.033843564484989486 1105 70-71 0.049423308687686074 1105 72-73 0.2617886283695654 1105 74-75 0.28956285358628264 1105 76-77 0.36961108548574373 1105 78-79 -0.017060161739568258 1105 80-81 0.02871598826794397 1105 82-83 0.2196684404554432 1105 84-85 0.148901430093904 1105 86-87 0.2085108133028335 1105 88-89 0.5087583963022162 1105 90-91 0.2478943933516824 1105 92-93 0.5262265054456847 1105 94-95 0.5056371183267565 1105 96-97 0.5487440313337721 1105 98-99 1.0076828535549573 1105 100-101 1.517456303979234 1106 1 0.05943084020171341 1106 2 0.08072026585558234 1106 3 0.06967468765591178 1106 4 0.027809789422889253 1106 5 0.005064689234352215 1106 6 0.10288824078593706 1106 7 0.05334667284263617 1106 8 -0.018652462067713316 1106 9 0.03627302362806972 1106 10-11 0.08862695547467325 1106 12-13 0.14437102071008923 1106 14-15 0.1361410577971469 1106 16-17 0.18030555637830759 1106 18-19 -0.08560136614797642 1106 20-21 -0.06561871689148546 1106 22-23 -0.026628141675033135 1106 24-25 0.024296538634779097 1106 26-27 0.08539556133521131 1106 28-29 0.17778476918524433 1106 30-31 0.18750353223214233 1106 32-33 0.1001177508896447 1106 34-35 0.15829948116028447 1106 36-37 0.07611901178376712 1106 38-39 0.1907927936029239 1106 40-41 0.3416610792926278 1106 42-43 0.15926429407350895 1106 44-45 0.2878199380607569 1106 46-47 0.24388418410108414 1106 48-49 0.325429401843671 1106 50-51 0.37858813973274863 1106 52-53 0.48799876408153153 1106 54-55 0.37621591149923717 1106 56-57 0.4014768729964615 1106 58-59 0.35864639043655444 1106 60-61 0.39083213792677185 1106 62-63 0.518781753104733 1106 64-65 0.5581846480041506 1106 66-67 0.31626241422332413 1106 68-69 0.5030246583943594 1106 70-71 0.4584209153761023 1106 72-73 0.5129370966769287 1106 74-75 0.6098436259780726 1106 76-77 0.6052712941375376 1106 78-79 0.42417386611814933 1106 80-81 0.3620632647382642 1106 82-83 0.5258858302466614 1106 84-85 0.5612494052827053 1106 86-87 0.6771143267056488 1106 88-89 0.7380133509122864 1106 90-91 0.6833358843794386 1106 92-93 0.7031732796953669 1106 94-95 1.0347433610149714 1106 96-97 1.2992570272298103 1106 98-99 1.6265637126842059 1106 100-101 2.254363029077684 1107 1 0.049191566410002 1107 2 -0.03591773180924207 1107 3 0.014234210095025901 1107 4 -0.05764598192674697 1107 5 -0.09164994182354746 1107 6 -0.14642814330194653 1107 7 0.005090000310609355 1107 8 -0.16918610018049662 1107 9 -0.059516086536547164 1107 10-11 -0.16784922403822833 1107 12-13 -0.1015665872097884 1107 14-15 -0.04949791011878801 1107 16-17 -0.27833905215003796 1107 18-19 -0.050875914323412985 1107 20-21 -0.15409838273043164 1107 22-23 -0.024439978738627133 1107 24-25 0.0578583469197369 1107 26-27 0.03580684913800525 1107 28-29 0.022632735769136048 1107 30-31 -0.029473888873290832 1107 32-33 -0.09032642678142366 1107 34-35 -0.06919579092115669 1107 36-37 -0.15205839616571382 1107 38-39 -0.06378827169991297 1107 40-41 -0.24122007316835692 1107 42-43 -0.21727996746672318 1107 44-45 -0.13787921831411865 1107 46-47 -0.24648796475845103 1107 48-49 -0.1435028285827471 1107 50-51 -0.39537755613464043 1107 52-53 -0.45746786092463054 1107 54-55 -0.2736132256350672 1107 56-57 -0.21334363054742056 1107 58-59 -0.2699197757559517 1107 60-61 -0.27973998133189326 1107 62-63 -0.48097025728846887 1107 64-65 -0.4298868545694816 1107 66-67 -0.4744386333189752 1107 68-69 -0.5212181346132496 1107 70-71 -0.5102041236806159 1107 72-73 -0.486569226400249 1107 74-75 -0.5260612565228797 1107 76-77 -0.5379114584155396 1107 78-79 -0.42889689643344653 1107 80-81 -0.5383912732413094 1107 82-83 -0.4437439942858923 1107 84-85 -0.34444218642247293 1107 86-87 -0.4431764197299124 1107 88-89 -0.36263457949275235 1107 90-91 -0.36860774238633454 1107 92-93 -0.14102313859797988 1107 94-95 -0.10455509809469632 1107 96-97 0.04473121690567794 1107 98-99 0.3087673338587038 1107 100-101 0.8586262138036282 1108 1 0.04386369458632089 1108 2 0.05300168675747585 1108 3 -0.01226232812950201 1108 4 -0.007635469728974442 1108 5 -0.07556772918716348 1108 6 0.09048562042735142 1108 7 0.0753738296505162 1108 8 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-1.0124585647728637 2316 56-57 -1.2533997274314146 2316 58-59 -1.039752215532154 2316 60-61 -0.9003126370226582 2316 62-63 -0.8883133978374573 2316 64-65 -0.8974137708478054 2316 66-67 -0.9208094278787442 2316 68-69 -0.9685752993832395 2316 70-71 -0.8908880966255524 2316 72-73 -0.9391182746523334 2316 74-75 -1.0567892012440758 2316 76-77 -0.8960608455862982 2316 78-79 -0.7821690685807603 2316 80-81 -0.9133992349084359 2316 82-83 -0.8542922536755491 2316 84-85 -1.2092560002801527 2316 86-87 -1.096706286938069 2316 88-89 -1.347893078024594 2316 90-91 -1.8843794397553317 2316 92-93 -1.655097320883634 2316 94-95 -1.487092021239043 2316 96-97 -1.6723838839664502 2316 98-99 -2.2655762303386275 2316 100-101 -2.294433257658543 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 16.0 5 51.0 6 122.0 7 253.0 8 526.0 9 882.0 10 1557.0 11 2313.0 12 3322.0 13 4453.0 14 5300.0 15 7053.0 16 9153.0 17 12179.0 18 11200.0 19 7837.0 20 7125.0 21 7218.0 22 7804.0 23 8689.0 24 10291.0 25 11894.0 26 13923.0 27 16757.0 28 20794.0 29 25053.0 30 30260.0 31 36418.0 32 44855.0 33 55724.0 34 73658.0 35 104656.0 36 147559.0 37 243812.0 38 243644.0 39 25226.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.9364012659685 22.79102009900181 15.992362145739868 21.28021648928982 2 13.972972343173678 23.77286981184836 40.74804591272853 21.506111932249432 3 17.938820756601288 29.12613683271415 32.11735509127951 20.81768731940505 4 11.530030359924899 19.670368446983318 40.502203757706496 28.297397435385292 5 11.103369278744259 40.09386763631036 36.56925277165924 12.23351031328615 6 25.904613681665534 40.91066843662482 19.107891155896418 14.07682672581323 7 23.886386636306742 35.785846016591435 23.864415638024475 16.46335170907735 8 22.405757733125608 36.16626053609236 22.35998482003755 19.067996910744483 9 24.353436796761606 19.068080134222825 23.56514400990692 33.013339059108645 10-11 21.589293798852182 28.37063409632618 29.878060959533414 20.16201114528822 12-13 24.611429579621564 26.643871755948815 29.136581379246064 19.608117285183557 14-15 22.17115074767973 28.090670315183957 28.611025113516824 21.127153823619487 16-17 18.58645856870649 31.322762815847373 30.216150062897952 19.874628552548188 18-19 18.252614881689503 30.99142465279165 32.211772127458424 18.544188338060426 20-21 19.287124329218763 29.847226660807735 31.41236900624509 19.453280003728413 22-23 19.126461404279684 29.43963967562817 30.667269204649863 20.766629715442285 24-25 19.435634277896003 29.968108630393843 31.454985481603792 19.14127161010637 26-27 20.074669192258607 28.925739857094886 32.08374234800987 18.915848602636643 28-29 18.796605147871475 28.739605387555095 33.152613550113855 19.31117591445958 30-31 18.92780696147752 30.49087704230416 32.156386902621875 18.424929093596454 32-33 18.812084714843074 30.350853539993878 31.300974380484426 19.536087364678625 34-35 19.331231250101435 29.3048768358723 31.463292125344307 19.90059978868196 36-37 20.08856444853232 29.05442468484291 31.66437070547743 19.19264016114734 38-39 20.292291769061453 28.651535058240697 32.2104414174199 18.845731755277953 40-41 19.014588576831397 29.06096440413887 33.06308510779966 18.861361911230073 42-43 18.59408298207117 30.024498533292327 31.96049439846259 19.420924086173912 44-45 18.827171282379066 30.44738953416759 30.849662269006316 19.87577691444703 46-47 19.10308777291195 29.832478623942848 29.92901834088174 21.135415262263457 48-49 19.40838724228359 29.499119064085694 30.577547744063356 20.514945949567355 50-51 20.25585404593189 29.775167518528672 30.456435195722975 19.51254323981646 52-53 19.12121832514414 29.996737639648995 31.486105008056033 19.395939027150828 54-55 18.58330337288113 29.724638477210082 31.435879638876685 20.256178511032104 56-57 19.3974618144473 30.786589511862427 29.676788726721025 20.139159946969247 58-59 18.8232317642036 29.73583093579365 30.59453435853989 20.84640294146286 60-61 19.24772795366024 29.52744765138653 30.465894337361433 20.758930057591797 62-63 20.51238518729353 29.168055467130056 30.458277105119812 19.861282240456603 64-65 19.990187792665314 29.056742272262536 30.905290560764737 20.047779374307414 66-67 18.83292093937601 29.51234310511427 30.787726556589522 20.867009398920203 68-69 18.679764410389314 30.394496916978547 30.60646764554366 20.31927102708848 70-71 19.001982801846733 29.989784305280743 29.906477499464245 21.101755393408276 72-73 19.040824445065844 29.36598689729557 29.43626912475532 22.15691953288326 74-75 19.290741599179082 29.67724136323954 29.71385984608586 21.318157191495523 76-77 19.87728125135318 28.798268930347515 30.494780476918514 20.82966934138079 78-79 19.027068425476095 28.678046953161427 30.88698981324491 21.407894808117568 80-81 18.812067000423195 30.3093982104314 29.977583578879212 20.900951210266197 82-83 19.029188833799044 29.90502029632003 29.536503207255492 21.529287662625435 84-85 19.16091592431324 28.862609688544456 29.383879945139086 22.59259444200322 86-87 19.35732322285621 29.110984480217674 30.061965827986924 21.46972646893919 88-89 20.293670611225707 27.93787528289754 30.36210884978663 21.406345256090127 90-91 19.29094734590847 28.442014834691808 30.86805545402787 21.39898236537185 92-93 18.992235642971902 29.286427334664634 30.1610254161082 21.56031160625526 94-95 18.957731522053027 29.161163500421527 29.489439330348933 22.391665647176513 96-97 19.132310931625586 28.514729533760686 29.067334119796186 23.28562541481754 98-99 19.41379046325517 28.302224397129123 29.264870371110135 23.01911476850557 100-101 20.031775133918707 27.995698955157362 29.927174355852372 22.04535155507156 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5451.0 1 3195.0 2 1078.0 3 1153.0 4 872.5 5 840.0 6 1598.0 7 3018.0 8 4065.5 9 4087.5 10 3087.5 11 2152.0 12 2025.5 13 1987.5 14 1936.0 15 1839.5 16 1791.5 17 1869.0 18 2036.0 19 2230.5 20 2453.5 21 2890.0 22 3357.0 23 3867.5 24 4525.5 25 5685.0 26 7904.5 27 10269.5 28 11710.5 29 14012.5 30 18278.5 31 22210.5 32 26960.5 33 34083.0 34 41076.0 35 45759.5 36 51138.5 37 56172.5 38 56359.0 39 54772.5 40 52270.0 41 49253.5 42 46093.5 43 44564.0 44 44176.5 45 43043.5 46 41981.5 47 42785.0 48 71705.5 49 71274.5 50 39168.5 51 29045.5 52 20611.0 53 16336.5 54 13721.0 55 11842.5 56 10132.0 57 8329.0 58 7020.5 59 5715.5 60 4190.5 61 3078.5 62 2266.5 63 1601.5 64 1117.0 65 793.5 66 571.0 67 454.5 68 327.5 69 201.0 70 151.5 71 126.5 72 114.0 73 110.0 74 99.5 75 80.5 76 50.5 77 32.0 78 24.0 79 18.0 80 13.5 81 7.0 82 3.0 83 2.0 84 2.0 85 2.0 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2345237619675362 2 0.0 3 0.0 4 0.0 5 0.02646506611273119 6 0.004077950438754178 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0028712100027963087 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.161173917096099E-4 26-27 0.001456410870983635 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006449819571498955 36-37 0.016561472190042476 38-39 0.012275463055433493 40-41 0.007989453920824511 42-43 0.006949160441550487 44-45 0.002996045220309192 46-47 4.993408700515319E-4 48-49 0.001747693045180362 50-51 4.1611739170961E-5 52-53 0.0 54-55 0.0 56-57 0.001414799131812674 58-59 4.161173917096099E-4 60-61 0.0019973634802061277 62-63 6.24176087564415E-4 64-65 0.001622857827667479 66-67 0.005243079135541086 68-69 2.4967043502576597E-4 70-71 1.2483521751288298E-4 72-73 0.0 74-75 4.161173917096099E-4 76-77 0.05892222266608078 78-79 0.1367777866549488 80-81 7.90623044248259E-4 82-83 9.57070000932103E-4 84-85 0.0 86-87 0.1036132305356929 88-89 0.345710329032344 90-91 0.38220382428527677 92-93 2.91282174196727E-4 94-95 2.4967043502576597E-4 96-97 1.2483521751288298E-4 98-99 0.0 100-101 0.001289963914299791 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1201584.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.80438967977607 #Duplication Level Percentage of deduplicated Percentage of total 1 68.24609596421574 21.022793342050942 2 13.431808267113471 8.27517311928402 3 5.792354839826374 5.352898669486454 4 3.182300344219502 3.9211527952569214 5 1.971174634834832 3.0360415789171227 6 1.362114393024493 2.517546153069488 7 0.9844013004627759 2.1226716882513568 8 0.7105517534392506 1.7510490480472671 9 0.5535806573668088 1.53474428598424 >10 3.1674060797824546 18.256386200742988 >50 0.33666551949504203 7.209577854607528 >100 0.24479431723683107 13.901844400671 >500 0.008916349297127377 1.8533905954757224 >1k 0.005674040461808331 4.107539037063156 >5k 0.0016211544176595232 3.1314044536367476 >10k+ 5.403848058865077E-4 2.005786777455067 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13212 1.0995485958534734 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10887 0.9060540087085048 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7500 0.624176087564415 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7476 0.6221787240842089 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6587 0.5481930518382402 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5564 0.46305543349445405 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5439 0.45265249870171376 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5057 0.4208611299750995 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4822 0.4013036125647479 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4699 0.3910671247286915 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4305 0.3582770742619742 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3450 0.2871210002796309 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3105 0.2584089002516678 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3002 0.24983688198244983 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2775 0.23094515239883354 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2465 0.20514587411283774 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2342 0.1949093862767813 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2195 0.1826755349605188 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2051 0.17069135407928201 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1978 0.16461604016032172 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1560 0.12982862621339833 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1533 0.12758159229816643 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1510 0.12566745229630222 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1430 0.11900957402894846 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1429 0.11892635055060652 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1387 0.11543096446024581 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1237 0.10294744270895752 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.3223478341922E-5 0.0 10-11 0.0 0.0 0.0 2.4967043502576597E-4 0.0 12-13 0.0 0.0 0.0 4.1611739170961E-4 0.0 14-15 0.0 0.0 0.0 5.409526092224929E-4 0.0 16-17 0.0 0.0 0.0 7.49011305077298E-4 0.0 18-19 0.0 0.0 0.0 9.570700009321029E-4 0.0 20-21 0.0 0.0 0.0 0.0010819052184449858 0.0 22-23 0.0 0.0 0.0 0.0019557517410351668 0.0 24-25 0.0 0.0 0.0 0.004410844352121865 0.0 26-27 0.0 0.0 0.0 0.008197512616679316 0.0 28-29 0.0 0.0 0.0 0.01847561219190668 0.0 30-31 0.0 0.0 0.0 0.04714610048069881 0.0 32-33 0.0 0.0 0.0 0.09483315357062011 0.0 34-35 0.0 0.0 0.0 0.172855164516172 0.0 36-37 0.0 0.0 0.0 0.2809208511431577 0.0 38-39 0.0 0.0 0.0 0.4330533695521911 0.0 40-41 0.0 0.0 0.0 0.6014144662378993 0.0 42-43 0.0 0.0 0.0 0.7676533642258885 0.0 44-45 0.0 0.0 0.0 0.965517183983808 0.0 46-47 0.0 0.0 0.0 1.2132318672685387 0.0 48-49 0.0 0.0 0.0 1.4821685375304599 0.0 50-51 0.0 0.0 0.0 1.7549334877961091 0.0 52-53 0.0 0.0 0.0 2.0066428980412523 0.0 54-55 0.0 0.0 0.0 2.2818213291788174 0.0 56-57 0.0 0.0 0.0 2.602439779491072 0.0 58-59 0.0 0.0 0.0 2.9793589129016365 0.0 60-61 0.0 0.0 0.0 3.4259360976843896 0.0 62-63 0.0 0.0 0.0 3.8416789837414616 0.0 64-65 0.0 0.0 0.0 4.238904645867455 0.0 66-67 0.0 0.0 0.0 4.688436264131346 0.0 68-69 0.0 0.0 0.0 5.189067098097178 0.0 70-71 0.0 0.0 0.0 5.7457073329871236 0.0 72-73 0.0 0.0 0.0 6.330019374425758 0.0 74-75 0.0 0.0 0.0 6.9182013076072915 0.0 76-77 0.0 0.0 0.0 7.508547051225715 0.0 78-79 0.0 0.0 0.0 8.17728931144223 0.0 80-81 0.0 0.0 0.0 8.897796575187419 0.0 82-83 0.0 0.0 0.0 9.611937242839453 0.0 84-85 0.0 0.0 0.0 10.395735961863673 0.0 86-87 0.0 0.0 0.0 11.230509061372322 0.0 88-89 0.0 0.0 0.0 12.019217965618717 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 230 0.0 28.886578 2 CCCGTGC 135 4.2717693E-7 28.11302 8 TTTACGC 70 0.008429286 27.118011 3 TACGCCG 70 0.008429286 27.118011 5 CGACGAG 35 0.008444639 27.108984 94-95 CGCCGAA 70 0.008442988 27.108984 7 GTGTAGC 375 0.0 25.369282 1 TTTTACG 95 0.0012331521 24.977116 2 CCGTGCC 140 1.7968492E-5 23.72036 9 CTGTGCG 100 0.001665752 23.72036 9 GTACACC 125 2.2700166E-4 22.779129 4 TAGCCCG 170 3.174313E-6 22.332481 5 GTACATG 6070 0.0 22.177225 1 GAGTACT 5575 0.0 21.786253 12-13 ATGGGAT 1395 0.0 21.772167 5 TGATACG 55 0.0027818466 21.718056 90-91 CTGGTCG 485 0.0 21.519503 9 ACATGGG 5990 0.0 20.91573 3 GTACTTT 5760 0.0 20.8806 14-15 AGCCCGT 205 6.9122143E-7 20.827633 6 >>END_MODULE