FastQCFastQC Report
Thu 26 May 2016
SRR938721_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938721_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4542482
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237310.522423644166339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164560.36226890937597556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119220.2624556354873833No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT89770.1976232376925214No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA77290.17014927081714357No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA62190.1369075320496592No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47910.10547097379802496No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47600.10478852750544745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG262900.025.1230891
GTATCAA396750.024.0449331
TACATGG270950.023.9628282
ACATGGG270650.022.9387593
GAGTACT182900.022.56522812-13
GTACTTT194550.021.27495414-15
ATCAACG457400.020.712063
GGTATCA296700.020.6310441
TCAACGC458800.020.6281974
TATCAAC460300.020.5403842
CAACGCA461800.020.5044525
AGTACTT192800.020.31348812-13
AACGCAG468000.020.2572426
CATGGGA161800.020.1226374
CATGGGG101250.019.6589764
ACTTTTT227900.018.5580116-17
ACGCAGA509200.018.5353247
CGCAGAG511400.018.4185458
AGTACAT202050.018.2250542
GCAGAGT521150.017.6925039
Produced by FastQC (version 0.11.5)