##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938721_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4542482 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.629523243020007 33.0 31.0 34.0 30.0 34.0 2 31.68331233893717 33.0 31.0 34.0 30.0 34.0 3 31.69327759581656 33.0 31.0 34.0 28.0 34.0 4 35.25475037655625 37.0 35.0 37.0 33.0 37.0 5 35.28990912897398 37.0 35.0 37.0 33.0 37.0 6 35.38667362908648 37.0 35.0 37.0 33.0 37.0 7 35.300384899709016 37.0 35.0 37.0 33.0 37.0 8 35.27297257314393 37.0 35.0 37.0 32.0 37.0 9 36.839758748631255 39.0 37.0 39.0 33.0 39.0 10-11 36.73326487589824 39.0 37.0 39.0 32.0 39.0 12-13 36.61420771727879 39.0 37.0 39.0 32.0 39.0 14-15 37.746292555479585 40.0 37.0 41.0 32.0 41.0 16-17 37.68443936596777 40.0 37.0 41.0 32.0 41.0 18-19 37.643978556216624 40.0 37.0 41.0 32.0 41.0 20-21 37.53922371073787 40.0 37.0 41.0 32.0 41.0 22-23 37.44695400003786 40.0 37.0 41.0 32.0 41.0 24-25 37.46015438256002 40.0 37.0 41.0 32.0 41.0 26-27 37.35980351710805 40.0 37.0 41.0 31.0 41.0 28-29 37.237877332260204 40.0 37.0 41.0 31.0 41.0 30-31 37.02564963383455 39.5 36.5 41.0 30.5 41.0 32-33 36.80805427517379 39.0 36.0 41.0 30.0 41.0 34-35 36.480281264735886 39.0 35.0 41.0 30.0 41.0 36-37 36.22287485123772 39.0 35.0 41.0 30.0 41.0 38-39 36.03965387204616 39.0 35.0 41.0 29.0 41.0 40-41 35.85379545807776 39.0 35.0 41.0 28.5 41.0 42-43 35.58063983522665 39.0 35.0 40.0 27.0 41.0 44-45 35.50954147974609 39.0 35.0 41.0 26.5 41.0 46-47 35.28922789347322 39.0 35.0 41.0 25.5 41.0 48-49 35.04276406598859 39.0 35.0 41.0 24.0 41.0 50-51 33.88971965106301 38.0 33.0 40.0 23.0 40.5 52-53 33.99087756429194 38.0 33.5 39.5 23.0 40.5 54-55 34.51180136762237 38.0 34.0 40.0 23.0 41.0 56-57 34.415634008015886 38.0 34.0 40.0 23.0 41.0 58-59 34.19247506099089 38.0 34.0 40.0 22.5 41.0 60-61 33.89139615743112 38.0 34.0 40.0 22.0 41.0 62-63 33.5233945891255 37.0 33.0 40.0 20.5 41.0 64-65 33.1188008009718 36.5 33.0 39.5 19.5 41.0 66-67 32.71655715972017 36.0 32.0 39.0 18.0 41.0 68-69 32.3172183180913 35.5 32.0 39.0 16.0 40.5 70-71 31.910686492538662 35.0 31.5 38.5 13.0 40.0 72-73 31.49054437640039 35.0 31.0 37.5 9.5 40.0 74-75 31.04226158738769 35.0 31.0 37.0 7.5 39.0 76-77 30.61501487512774 35.0 30.5 36.5 4.5 39.0 78-79 30.20285969212426 34.5 30.0 36.0 2.0 38.5 80-81 29.778370943462185 34.0 30.0 35.5 2.0 37.0 82-83 29.337755658690558 34.0 29.0 35.0 2.0 37.0 84-85 28.93817322776403 34.0 29.0 35.0 2.0 36.5 86-87 28.56966741970579 34.0 29.0 35.0 2.0 36.0 88-89 28.197313715277243 34.0 28.5 35.0 2.0 36.0 90-91 27.786788258049235 34.0 27.0 35.0 2.0 35.0 92-93 27.35138014856195 34.0 26.5 35.0 2.0 35.0 94-95 26.856820676449573 33.5 25.5 35.0 2.0 35.0 96-97 26.278729888197685 33.0 24.5 35.0 2.0 35.0 98-99 25.58268970575998 33.0 21.5 35.0 2.0 35.0 100-101 23.324793141722964 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25309004815852276 1101 2 0.13283139221259788 1101 3 0.21220479903237788 1101 4 0.2532684345756451 1101 5 0.10370956755087235 1101 6 0.07520902160249676 1101 7 0.1533849491260284 1101 8 0.22282034170649467 1101 9 0.29388695889223015 1101 10-11 0.2935225889387567 1101 12-13 0.22410307795367856 1101 14-15 0.3392769776684901 1101 16-17 0.35581272302853506 1101 18-19 0.4497971739839457 1101 20-21 0.517030708466244 1101 22-23 0.5078739433627817 1101 24-25 0.4982797578164835 1101 26-27 0.4846883416351062 1101 28-29 0.41300288422992537 1101 30-31 0.387210732558124 1101 32-33 0.43075775241779724 1101 34-35 0.4080481761874495 1101 36-37 0.5210401375074767 1101 38-39 0.5035497889389475 1101 40-41 0.4822460084197857 1101 42-43 0.5107969813439084 1101 44-45 0.54490473142517 1101 46-47 0.5584620164669118 1101 48-49 0.5857857111603764 1101 50-51 0.41917446563730465 1101 52-53 0.4796367111161075 1101 54-55 0.4745443854960101 1101 56-57 0.41029823054785197 1101 58-59 0.4483514698067097 1101 60-61 0.3206324693226392 1101 62-63 0.312251632328568 1101 64-65 0.25867986930763465 1101 66-67 0.31648587278123586 1101 68-69 0.24433300280725945 1101 70-71 0.037336751792047096 1101 72-73 -0.01958995829959065 1101 74-75 0.06661434581369718 1101 76-77 -0.05973908844817899 1101 78-79 -0.17883790756873807 1101 80-81 -0.24770124595402976 1101 82-83 -0.4857874660732051 1101 84-85 -0.45741806509261096 1101 86-87 -0.443600662856781 1101 88-89 -0.5005497751027441 1101 90-91 -0.589337036025789 1101 92-93 -0.6518100947355379 1101 94-95 -0.7385382145273667 1101 96-97 -0.8970993306779036 1101 98-99 -0.8837593799417256 1101 100-101 -0.6283035338674523 1102 1 0.21899907416229425 1102 2 0.18500488444384544 1102 3 0.27279796511491483 1102 4 0.3219501016059496 1102 5 0.21565270716761376 1102 6 0.2206832878894005 1102 7 0.2761845679189108 1102 8 0.24355182777558326 1102 9 0.30611932699865463 1102 10-11 0.2344040457589074 1102 12-13 0.2726265546232227 1102 14-15 0.16915868071346551 1102 16-17 0.3403538076286239 1102 18-19 0.3964456501991549 1102 20-21 0.4098662879217301 1102 22-23 0.3165382271907333 1102 24-25 0.3480499566375812 1102 26-27 0.369016547171249 1102 28-29 0.4042807775507171 1102 30-31 0.4363548079330357 1102 32-33 0.3917281326414681 1102 34-35 0.36276324131448945 1102 36-37 0.4577743001681043 1102 38-39 0.3785867460280201 1102 40-41 0.47875607533872966 1102 42-43 0.43720104697160167 1102 44-45 0.5622091387248531 1102 46-47 0.5007099639270436 1102 48-49 0.5078643305068766 1102 50-51 0.5023450250827395 1102 52-53 0.5097206643298549 1102 54-55 0.3507626972909961 1102 56-57 0.27718406603663936 1102 58-59 0.31158386273293104 1102 60-61 0.29660709020028264 1102 62-63 0.3569964510926553 1102 64-65 0.4255278468215167 1102 66-67 0.3874751056637038 1102 68-69 0.3127963023797875 1102 70-71 0.1220322747061715 1102 72-73 0.10559355500148371 1102 74-75 0.02464376215079156 1102 76-77 0.05904032080125532 1102 78-79 0.003945598089085678 1102 80-81 -0.07413855349028964 1102 82-83 0.04383319296814392 1102 84-85 -0.1077871530877097 1102 86-87 -0.12957281110136165 1102 88-89 -0.052711843997716556 1102 90-91 -0.0525348629749125 1102 92-93 -0.22081488640971259 1102 94-95 -0.25181059951540163 1102 96-97 -0.17021263428494748 1102 98-99 -0.23997054903607662 1102 100-101 -0.14449793038869174 1103 1 0.1286021591413551 1103 2 0.17830775344141614 1103 3 0.1526342500985578 1103 4 0.194308258724881 1103 5 0.1559845883435429 1103 6 0.24473013567941848 1103 7 0.17225845751801927 1103 8 0.07200440571553912 1103 9 0.32024504811364807 1103 10-11 0.3045881588256165 1103 12-13 0.26245874963324667 1103 14-15 0.3777982201408818 1103 16-17 0.2746309422303099 1103 18-19 0.4795089829198318 1103 20-21 0.38224127025836196 1103 22-23 0.38629533623384305 1103 24-25 0.3344000259411999 1103 26-27 0.3756479732890341 1103 28-29 0.3664658546345123 1103 30-31 0.28383889898238834 1103 32-33 0.18276615737438107 1103 34-35 0.23475613523793726 1103 36-37 0.3685467559135489 1103 38-39 0.3512284083242889 1103 40-41 0.2563820386757172 1103 42-43 0.30127385959095676 1103 44-45 0.37524327267151847 1103 46-47 0.3371375240585408 1103 48-49 0.3840751878452551 1103 50-51 0.20261260667091818 1103 52-53 0.24073339060947063 1103 54-55 0.3279246017439661 1103 56-57 0.27518449747108775 1103 58-59 0.2875216768941229 1103 60-61 0.28160277352409224 1103 62-63 0.18396667916270815 1103 64-65 0.048819331981469816 1103 66-67 0.10825196436187667 1103 68-69 0.03592537522117567 1103 70-71 0.036595171665627646 1103 72-73 0.06510986341503155 1103 74-75 0.08049021957710778 1103 76-77 0.10150132523721922 1103 78-79 0.12264354490989149 1103 80-81 0.030295655685357303 1103 82-83 0.04320741612317747 1103 84-85 0.1614584579374032 1103 86-87 -0.06494210804821421 1103 88-89 -0.0634428300119545 1103 90-91 -0.12637248889783237 1103 92-93 -0.06358371926659245 1103 94-95 -0.15083284035976519 1103 96-97 -0.13863361273535446 1103 98-99 -0.10701376134581508 1103 100-101 0.03432900216788681 1104 1 0.18044256295948102 1104 2 0.12714123267785737 1104 3 0.13640142659819432 1104 4 0.23081514072462994 1104 5 0.1422439560479063 1104 6 0.19726414281327465 1104 7 0.0854641358184054 1104 8 0.16848904606682424 1104 9 0.2112375518868319 1104 10-11 0.1792265153886774 1104 12-13 0.2975968195742027 1104 14-15 0.323755625924278 1104 16-17 0.32210918610881123 1104 18-19 0.16811388560475393 1104 20-21 0.19077107501126278 1104 22-23 0.31882968175355586 1104 24-25 0.2930911597623407 1104 26-27 0.3320956501437493 1104 28-29 0.3539277817120521 1104 30-31 0.26960791669312556 1104 32-33 0.3218843465272272 1104 34-35 0.4056715819487948 1104 36-37 0.42162028215674496 1104 38-39 0.2904148472222019 1104 40-41 0.07902570603973658 1104 42-43 0.1814541098964213 1104 44-45 0.380123921245783 1104 46-47 0.38744613866608546 1104 48-49 0.4333991305583851 1104 50-51 0.43276899716263273 1104 52-53 0.3956100698557421 1104 54-55 0.41151955222228054 1104 56-57 0.42268157953785845 1104 58-59 0.42105840798254235 1104 60-61 0.4263966116826978 1104 62-63 0.4228038017212725 1104 64-65 0.3088801341426759 1104 66-67 0.2909419235301556 1104 68-69 0.3737575897920564 1104 70-71 0.5031941405275759 1104 72-73 0.24254081116417936 1104 74-75 0.1503138199582672 1104 76-77 -0.035165165530703035 1104 78-79 0.24064021500735677 1104 80-81 0.11743839617647112 1104 82-83 0.0824336617841901 1104 84-85 0.17848406379123105 1104 86-87 0.1558481384746493 1104 88-89 0.11051393696496703 1104 90-91 0.15359233960630547 1104 92-93 0.13832627288089583 1104 94-95 0.14839705326585317 1104 96-97 0.33950405593980904 1104 98-99 0.3109152367385377 1104 100-101 0.46328899232168297 1105 1 0.27581393468254234 1105 2 0.22433471048655917 1105 3 0.24442842225344208 1105 4 0.3160937922715803 1105 5 0.2346234689208302 1105 6 0.22650170982512208 1105 7 0.35766412496109723 1105 8 0.3034436863148571 1105 9 0.2729434969939959 1105 10-11 0.29212008511849774 1105 12-13 0.3561587967994768 1105 14-15 0.3964911913489999 1105 16-17 0.22995939980865643 1105 18-19 0.29110650385637626 1105 20-21 0.2811249073049211 1105 22-23 0.35863924582051254 1105 24-25 0.3491311006205109 1105 26-27 0.355841204290293 1105 28-29 0.3381753856326313 1105 30-31 0.28347644813265305 1105 32-33 0.32989183867854877 1105 34-35 0.4212586785891901 1105 36-37 0.42188004931022505 1105 38-39 0.4707478432470822 1105 40-41 0.3665295411697116 1105 42-43 0.2582845724481686 1105 44-45 0.39674269072260415 1105 46-47 0.509861123822688 1105 48-49 0.3590236849056083 1105 50-51 0.4691218358297675 1105 52-53 0.4291950578608592 1105 54-55 0.4170832355085494 1105 56-57 0.4566431422336166 1105 58-59 0.4668085350069333 1105 60-61 0.5962882948030668 1105 62-63 0.46465078811731075 1105 64-65 0.45958320572344036 1105 66-67 0.2894658178385541 1105 68-69 0.2999375899018517 1105 70-71 0.3162065459149943 1105 72-73 0.4130565606629446 1105 74-75 0.28050413084930526 1105 76-77 0.2565798048831347 1105 78-79 0.16464905883232817 1105 80-81 0.24847670538889233 1105 82-83 0.2845115397785669 1105 84-85 0.18770614084272808 1105 86-87 0.13402876501442051 1105 88-89 0.14877198890802745 1105 90-91 0.17476111338335087 1105 92-93 0.2459795149152626 1105 94-95 0.2872553678581369 1105 96-97 0.24212133772167732 1105 98-99 0.28936241624824177 1105 100-101 0.45307565235197345 1106 1 0.16355799558546025 1106 2 0.17256686904282148 1106 3 0.2774702350426672 1106 4 0.2670425020456122 1106 5 0.1552185379486417 1106 6 0.14169809371443876 1106 7 0.16970402004268692 1106 8 0.19700547804311697 1106 9 0.30440151030684603 1106 10-11 0.25950577138732456 1106 12-13 0.21561304715451257 1106 14-15 0.3137112519234435 1106 16-17 0.3407683028992139 1106 18-19 0.42615695840621726 1106 20-21 0.38142415664555784 1106 22-23 0.2932204175060633 1106 24-25 0.27965737128296553 1106 26-27 0.05713287941032519 1106 28-29 0.21433742226712837 1106 30-31 0.22174298775722434 1106 32-33 0.21249896704419768 1106 34-35 0.26598655249387804 1106 36-37 0.27209778399996054 1106 38-39 0.239658034631411 1106 40-41 0.2819420898369813 1106 42-43 0.40702103497799413 1106 44-45 0.4141496915631109 1106 46-47 0.43302028148047356 1106 48-49 0.5781879000946191 1106 50-51 0.4765870683216278 1106 52-53 0.4797133098969084 1106 54-55 0.48840825313987324 1106 56-57 0.5077449787888924 1106 58-59 0.5144559618007349 1106 60-61 0.502253295431025 1106 62-63 0.33758756173119764 1106 64-65 0.31368526448161305 1106 66-67 0.28949449891942436 1106 68-69 0.47193289948845774 1106 70-71 0.505606742999305 1106 72-73 0.478001094471594 1106 74-75 0.4005604182252789 1106 76-77 0.35152897596971044 1106 78-79 0.5248290914864917 1106 80-81 0.3363968983189771 1106 82-83 0.35369996783796154 1106 84-85 0.38153982921765106 1106 86-87 0.6330883274679238 1106 88-89 0.6634805528976351 1106 90-91 0.6890304039764388 1106 92-93 0.7481357283846215 1106 94-95 0.7888328273061092 1106 96-97 0.7685583504452076 1106 98-99 0.8759140687615492 1106 100-101 1.0897950439024662 1107 1 0.014769458805076852 1107 2 0.06902464049038315 1107 3 0.07831926960550106 1107 4 0.07921716080976182 1107 5 0.11924393696153857 1107 6 0.09148854337303192 1107 7 -0.0672936274367899 1107 8 0.02686227264806007 1107 9 0.009641413332964532 1107 10-11 0.018528798541751712 1107 12-13 0.12223887917958365 1107 14-15 0.17615354886907397 1107 16-17 0.1152991554106606 1107 18-19 0.13369817586025334 1107 20-21 0.18552354501390056 1107 22-23 0.2745417853856509 1107 24-25 0.23348779938566366 1107 26-27 0.22200279422074942 1107 28-29 0.12814772114497686 1107 30-31 0.14083244766808178 1107 32-33 0.17989333745192937 1107 34-35 0.17261677502234818 1107 36-37 0.3022405631708551 1107 38-39 0.41616998587767995 1107 40-41 0.3429709258740701 1107 42-43 0.38707231408456977 1107 44-45 0.3687153069952629 1107 46-47 0.42148926558309796 1107 48-49 0.45069890397878964 1107 50-51 0.5654278441995544 1107 52-53 0.5242860705033792 1107 54-55 0.5172657247703114 1107 56-57 0.44922104827605835 1107 58-59 0.5259205528347195 1107 60-61 0.41009162170403357 1107 62-63 0.37115611751801936 1107 64-65 0.3521056225256842 1107 66-67 0.47450022433319106 1107 68-69 0.39051140839851683 1107 70-71 0.508040956485516 1107 72-73 0.43161347734875477 1107 74-75 0.48548619701179163 1107 76-77 0.39510336569960103 1107 78-79 0.343126381253402 1107 80-81 0.35827889599211105 1107 82-83 0.5534645184595774 1107 84-85 0.42238919627478566 1107 86-87 0.37824567620373983 1107 88-89 0.39999517100474336 1107 90-91 0.4684121461124029 1107 92-93 0.519884950328219 1107 94-95 0.49072960864285875 1107 96-97 0.5555768799482053 1107 98-99 0.7515151237452535 1107 100-101 0.9665467561001329 1108 1 0.16601244729775289 1108 2 0.10549029884596806 1108 3 0.07216128937760047 1108 4 0.09118309453052831 1108 5 0.12969798608912697 1108 6 0.11572058366679983 1108 7 0.09725032928030686 1108 8 0.12242500991914795 1108 9 0.1597554141352049 1108 10-11 0.17923720265646637 1108 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13658.0 11 17380.0 12 22552.0 13 28498.0 14 30169.0 15 29938.0 16 37362.0 17 47169.0 18 39493.0 19 29160.0 20 29288.0 21 31537.0 22 34741.0 23 38211.0 24 44211.0 25 50765.0 26 59060.0 27 69050.0 28 80545.0 29 96143.0 30 114844.0 31 139134.0 32 172210.0 33 218286.0 34 288129.0 35 406734.0 36 590811.0 37 858833.0 38 746330.0 39 117241.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.350800460921555 18.97936657242735 14.156558099620264 22.513274867030837 2 15.29446035488121 20.83652931492771 40.24460083851687 23.624409491674214 3 19.484716711409277 25.8192040104421 29.40680199323485 25.28927728491378 4 12.615803994852175 16.53169790032262 37.52897854485835 33.323519559966854 5 13.589190857193238 37.932486940421875 33.57290220194406 14.90542000044083 6 30.924809726767222 36.2024900527605 17.905845939850337 14.966854280621938 7 28.402827667588287 33.11156196083216 20.942055190524872 17.54355518105468 8 26.199541890582328 34.11829354241917 20.212998854726454 19.469165712272048 9 27.216600699455796 16.925517582532063 20.65892180556415 35.19895991244799 10-11 25.052676554831077 26.630297250599888 27.053581752449375 21.26344444211966 12-13 26.47250151781574 24.26760779134766 27.489969421230125 21.769921269606474 14-15 23.37341091895797 25.249011091631502 26.605004889480128 24.772573099930405 16-17 22.005830110136905 28.566570592048773 27.055270197336377 22.37232910047794 18-19 21.78031423075445 27.17478101453053 29.735836439750372 21.309068314964644 20-21 23.027949399084672 26.131416700742527 29.339782214791672 21.500851685381132 22-23 22.544955856176067 26.11758931776405 29.23350214278484 22.103952683275043 24-25 22.436054062807422 26.324233634698196 29.409623038714024 21.830089263780366 26-27 22.177173974830755 26.347408098417308 29.64383743941566 21.83158048733628 28-29 21.85223689752958 26.54935600192459 29.764753001887577 21.83365409865825 30-31 21.94516072327788 26.496167339183103 29.49103524356197 22.067636693977047 32-33 21.76416528594327 26.670796447507456 29.374415291862626 22.190622974686647 34-35 22.44713551924344 26.502342172709625 29.15876963512623 21.891752672920703 36-37 22.434135333938983 26.244534892915144 29.20294450873892 22.118385264406953 38-39 22.289921782609966 26.526744987249764 29.39296167321353 21.790371556926747 40-41 22.15847271483624 26.391522294389784 29.509026115951986 21.940978874821994 42-43 21.957188349964568 26.683150291896606 28.90526636788714 22.454394990251686 44-45 22.13175681802399 26.748522320992173 28.70665987263851 22.413060988345322 46-47 22.209730484774877 26.873044618281956 28.33259853608927 22.584626360853896 48-49 22.482854314399855 26.89146682306657 28.134607138705782 22.491071723827798 50-51 22.267723320344306 27.13509734471585 28.19782586314069 22.399353471799156 52-53 22.45349675144136 27.22851520941226 27.968271754416307 22.34971628473007 54-55 22.02228854035683 26.917829737509123 28.28997152807443 22.769910194059612 56-57 22.27860993322438 27.382536801328655 27.8214241411586 22.517429124288356 58-59 22.478041559459648 26.770412222114263 27.985920688194177 22.765625530231908 60-61 22.425404792914303 26.794746018594296 28.379363059070386 22.40048612942102 62-63 22.402176493345568 26.871291921418116 28.301423453272584 22.425108131963732 64-65 22.157983764347332 26.838003058743602 28.29316819760541 22.71084497930365 66-67 22.088703586785847 27.118041529714237 27.92845187599576 22.86480300750416 68-69 22.004924162433863 27.058335627787567 28.14696336126244 22.78977684851613 70-71 22.16437700949247 26.942746292180118 28.013881966120728 22.878994732206678 72-73 22.211966479390288 26.816786408148207 27.9004269989896 23.0708201134719 74-75 22.077848105535804 27.330649231173176 27.698879173706786 22.89262348958424 76-77 22.329536784138533 26.874946092687402 27.93431601295987 22.86120111021419 78-79 22.057592987283968 27.007805308069383 27.980155018328983 22.954446686317667 80-81 22.102998479284604 27.309605161256084 27.755659565388275 22.831736794071034 82-83 22.28472275357441 27.114692019426776 27.63751308600652 22.963072140992296 84-85 22.100949594418598 26.909778523794927 27.645876457305068 23.343395424481407 86-87 22.188715340170845 27.025715312525918 27.844414342188927 22.94115500511431 88-89 22.299231470557398 26.785836727417344 27.66950402800025 23.245427774025003 90-91 22.164739857862482 26.83479087753145 27.90674474692946 23.093724517676613 92-93 22.411043377682887 27.019425870532093 27.69695677361686 22.872573978168155 94-95 22.28013223864452 26.81816522751882 27.643982068811436 23.257720465025223 96-97 22.367356915800812 26.520719640577887 27.699747156897665 23.412176286723636 98-99 22.310660077679618 26.567820801141018 27.46722593868402 23.65429318249534 100-101 22.69729810605009 26.148637508123727 27.844643226609715 23.309421159216466 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5902.0 1 3573.0 2 1652.0 3 2087.5 4 1813.0 5 1796.5 6 3139.0 7 6098.0 8 9359.0 9 10307.5 10 8608.5 11 7142.0 12 7112.5 13 7429.0 14 7745.5 15 7997.5 16 8364.5 17 8712.0 18 8814.0 19 9038.5 20 9681.0 21 10723.0 22 12139.5 23 14011.0 24 15975.5 25 19209.0 26 24755.0 27 30615.5 28 35374.5 29 40900.0 30 49045.0 31 58760.0 32 68715.0 33 77805.0 34 87277.5 35 95419.5 36 106329.0 37 117981.5 38 122907.0 39 124557.0 40 124110.5 41 127006.5 42 132076.0 43 138169.0 44 144660.5 45 149244.5 46 152185.5 47 161570.0 48 197580.5 49 201951.5 50 172597.5 51 161776.0 52 155166.0 53 154474.5 54 151446.0 55 140277.0 56 128434.5 57 116226.5 58 107070.0 59 97783.0 60 84730.5 61 69694.0 62 55260.5 63 42443.5 64 30938.0 65 23183.5 66 17909.5 67 14248.0 68 10881.5 69 7888.0 70 6024.5 71 4662.0 72 3556.5 73 3000.5 74 2464.5 75 1899.5 76 1322.5 77 782.5 78 492.0 79 367.0 80 256.5 81 186.0 82 135.0 83 105.5 84 74.5 85 55.0 86 41.5 87 30.0 88 25.0 89 15.5 90 13.5 91 14.5 92 12.0 93 9.0 94 5.5 95 5.5 96 5.0 97 8.0 98 6.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15894394298095182 2 0.09314291173856054 3 0.08651657838159843 4 0.10219082871434602 5 0.12288436145701843 6 0.03907555384919522 7 0.04226764134673511 8 0.04583397358536589 9 0.04612016074031774 10-11 0.04484332574130178 12-13 0.04932325543612501 14-15 0.0467145494467562 16-17 0.045955052766307056 18-19 0.050269874487119594 20-21 0.052097069399504495 22-23 0.06484340499312931 24-25 0.08116708002365226 26-27 0.08295024614296766 28-29 0.07348405563302178 30-31 0.06199254064187817 32-33 0.050401960866328144 34-35 0.2939802513251566 36-37 0.4663639833905781 38-39 0.44499901155359556 40-41 0.4212564848908592 42-43 0.4495009556449536 44-45 0.4593083693011882 46-47 0.4584828294311348 48-49 0.47534585717675926 50-51 0.4645367884781932 52-53 0.47110808584381847 54-55 0.4625334783935302 56-57 0.44079426181545683 58-59 0.420640081787886 60-61 0.3909976968538345 62-63 0.44789390469791623 64-65 0.484283702169871 66-67 0.4855495299706196 68-69 0.4958852891436884 70-71 0.4925280936721378 72-73 0.4838654286357106 74-75 0.47695290812379665 76-77 0.458603908612076 78-79 0.43426699324290113 80-81 0.43895605970480456 82-83 0.45076678344570215 84-85 0.4489505957315846 86-87 0.45915426852544494 88-89 0.4706677979131233 90-91 0.47503765562527267 92-93 0.4767217569601817 94-95 0.4813888090255503 96-97 0.48193916893891925 98-99 0.4756210371334437 100-101 0.46242340641085644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4542482.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.503704100734154 #Duplication Level Percentage of deduplicated Percentage of total 1 66.24748932539434 23.520312584253446 2 14.104041300275352 10.014914178990196 3 6.478266625612021 6.900073840841721 4 3.611882708644805 5.1294085973713335 5 2.2101515258903217 3.9234282896498023 6 1.5312951436691955 3.2619989803033387 7 1.0742173282376704 2.669708591314202 8 0.7879240648779839 2.237937828262048 9 0.5955218301634307 1.9028907759285092 >10 2.979990956631742 18.85694732512925 >50 0.2214067755209802 5.513496032437131 >100 0.14407539847239953 9.633230300548956 >500 0.009572400909406045 2.3201771008167835 >1k 0.0037916650406991403 2.459584010008936 >5k 1.8647532987044793E-4 0.5059215036917649 >10k+ 1.8647532987044793E-4 1.149970060452527 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23731 0.522423644166339 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16456 0.36226890937597556 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11922 0.2624556354873833 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8977 0.1976232376925214 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7729 0.17014927081714357 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6219 0.1369075320496592 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4791 0.10547097379802496 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4760 0.10478852750544745 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.201439653475787E-5 0.0 2 0.0 0.0 0.0 6.604318960427361E-5 0.0 3 0.0 0.0 0.0 1.1007198267378936E-4 0.0 4 0.0 0.0 0.0 1.9812956881282083E-4 0.0 5 0.0 0.0 0.0 2.4215836188233656E-4 0.0 6 0.0 0.0 0.0 3.082015514866102E-4 0.0 7 0.0 0.0 0.0 3.082015514866102E-4 0.0 8 0.0 0.0 0.0 3.082015514866102E-4 0.0 9 0.0 0.0 0.0 6.384174995079782E-4 0.0 10-11 0.0 0.0 0.0 9.90647844064104E-4 0.0 12-13 0.0 0.0 0.0 0.001309856593818093 0.0 14-15 0.0 0.0 0.0 0.0013538853868876092 0.0 16-17 0.0 0.0 0.0 0.0015740293522351879 0.0 18-19 0.0 0.0 0.0 0.0019702884898608296 0.0 20-21 0.0 0.0 0.0 0.00237755482575385 0.0 22-23 0.0 0.0 0.0 0.0031370515062029963 0.0 24-25 0.0 0.0 0.0 0.003951584177989038 0.0 26-27 0.0 0.0 0.0 0.0051843903839354786 0.0 28-29 0.0 0.0 0.0 0.009036909777518106 0.0 30-31 0.0 0.0 0.0 0.02088065511321784 0.0 32-33 0.0 0.0 0.0 0.04356649074228583 0.0 34-35 0.0 0.0 0.0 0.07980218743849729 0.0 36-37 0.0 0.0 0.0 0.1368635032565897 0.0 38-39 0.0 0.0 0.0 0.22204821064783525 0.0 40-41 0.0 0.0 0.0 0.3166220581611551 0.0 42-43 0.0 0.0 0.0 0.4124727406734908 0.0 44-45 0.0 0.0 0.0 0.5282354448515151 0.0 46-47 0.0 0.0 0.0 0.6689844890964896 0.0 48-49 0.0 0.0 0.0 0.8255728916482223 0.0 50-51 0.0 0.0 0.0 0.9930143916915906 0.0 52-53 0.0 0.0 0.0 1.1576710705733122 0.0 54-55 0.0 0.0 0.0 1.3368572511679737 0.0 56-57 0.0 0.0 0.0 1.5509582646667615 0.0 58-59 0.0 0.0 0.0 1.7906069853441355 0.0 60-61 0.0 0.0 0.0 2.063002120866962 0.0 62-63 0.0 0.0 0.0 2.330576103548677 0.0 64-65 0.0 0.0 0.0 2.5998562019618348 0.0 66-67 0.0 0.0 0.0 2.9099840131452366 0.0 68-69 0.0 0.0 0.0 3.261245724253833 0.0 70-71 0.0 0.0 0.0 3.6476864410249727 0.0 72-73 0.0 0.0 0.0 4.040137088050102 0.0 74-75 0.0 0.0 0.0 4.44417831485078 0.0 76-77 0.0 0.0 0.0 4.866095231637682 0.0 78-79 0.0 0.0 0.0 5.339790009074334 0.0 80-81 0.0 0.0 0.0 5.8604525015178925 0.0 82-83 0.0 0.0 0.0 6.39233132899591 0.0 84-85 0.0 0.0 0.0 6.932036274442034 0.0 86-87 0.0 0.0 0.0 7.49130101120929 0.0 88-89 0.0 0.0 0.0 8.07646568549969 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 26290 0.0 25.123089 1 GTATCAA 39675 0.0 24.044933 1 TACATGG 27095 0.0 23.962828 2 ACATGGG 27065 0.0 22.938759 3 GAGTACT 18290 0.0 22.565228 12-13 GTACTTT 19455 0.0 21.274954 14-15 ATCAACG 45740 0.0 20.71206 3 GGTATCA 29670 0.0 20.631044 1 TCAACGC 45880 0.0 20.628197 4 TATCAAC 46030 0.0 20.540384 2 CAACGCA 46180 0.0 20.504452 5 AGTACTT 19280 0.0 20.313488 12-13 AACGCAG 46800 0.0 20.257242 6 CATGGGA 16180 0.0 20.122637 4 CATGGGG 10125 0.0 19.658976 4 ACTTTTT 22790 0.0 18.55801 16-17 ACGCAGA 50920 0.0 18.535324 7 CGCAGAG 51140 0.0 18.418545 8 AGTACAT 20205 0.0 18.225054 2 GCAGAGT 52115 0.0 17.692503 9 >>END_MODULE