FastQCFastQC Report
Thu 26 May 2016
SRR938721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4542482
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226200.497965649616223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175080.3854280545305408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131160.28874082494988423No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT92930.2045797869975049No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA74010.162928548753743No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA63520.139835446788782No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50400.11095255853517966No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45490.10014348983661354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG259550.024.497871
TACATGG268100.023.3277422
ACATGGG267100.022.1714843
GTATCAA406450.022.088811
GAGTACT195950.021.66320212-13
AGTACTT204300.020.46421612-13
GTACTTT209900.020.31389414-15
GGTATCA314150.020.2855661
CATGGGA161750.020.1542454
ATCAACG476600.018.6781083
TCAACGC479400.018.5591184
TATCAAC482550.018.5068042
CAACGCA482400.018.44375
CATGGGG99250.018.2158134
AACGCAG489800.018.2134916
ATGGGAG58950.017.9504415
ACTTTTT243000.017.71246516-17
TACTTTT235150.017.49692314-15
ATGGGAT73800.017.038985
CGTACAC3903.0559022E-1017.0340353
Produced by FastQC (version 0.11.5)