##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938721_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4542482 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97444238634297 31.0 31.0 34.0 30.0 34.0 2 32.196420811353796 34.0 31.0 34.0 30.0 34.0 3 32.28002026205057 34.0 31.0 34.0 30.0 34.0 4 35.91941586119659 37.0 35.0 37.0 35.0 37.0 5 35.83115464188961 37.0 35.0 37.0 35.0 37.0 6 35.86291393119444 37.0 35.0 37.0 35.0 37.0 7 35.79664465373776 37.0 35.0 37.0 33.0 37.0 8 35.76436516424281 37.0 35.0 37.0 33.0 37.0 9 37.40710276892677 39.0 37.0 39.0 34.0 39.0 10-11 37.33085843818424 39.0 37.0 39.0 34.0 39.0 12-13 37.25785374603576 39.0 37.0 39.0 33.0 39.0 14-15 38.45227388903247 40.0 38.0 41.0 33.5 41.0 16-17 38.387854041028675 40.0 38.0 41.0 33.0 41.0 18-19 38.42769800738891 40.0 38.0 41.0 33.5 41.0 20-21 38.508845494599655 40.0 38.0 41.0 34.0 41.0 22-23 38.45752013987067 40.0 38.0 41.0 34.0 41.0 24-25 38.38170894237996 40.0 38.0 41.0 34.0 41.0 26-27 38.21084167201983 40.0 38.0 41.0 33.0 41.0 28-29 38.04294568476001 40.0 37.0 41.0 33.0 41.0 30-31 37.81145384835867 40.0 37.0 41.0 33.0 41.0 32-33 37.58106097063235 40.0 36.5 41.0 32.0 41.0 34-35 37.53985332247877 40.0 36.0 41.0 32.0 41.0 36-37 37.44695994392493 40.0 36.0 41.0 32.0 41.0 38-39 37.3329779842826 40.0 36.0 41.0 31.5 41.0 40-41 37.1676642857363 40.0 36.0 41.0 31.0 41.0 42-43 36.97507386930758 40.0 36.0 41.0 31.0 41.0 44-45 36.69755884558266 39.5 35.5 41.0 30.5 41.0 46-47 36.37775592726619 39.0 35.0 41.0 30.0 41.0 48-49 36.08096267635183 39.0 35.0 41.0 29.0 41.0 50-51 35.83313890952127 39.0 35.0 41.0 28.0 41.0 52-53 35.614559617407394 39.0 35.0 40.0 28.0 41.0 54-55 35.370910660735696 38.5 35.0 40.0 27.5 41.0 56-57 35.11215201293038 38.0 34.0 40.0 27.0 41.0 58-59 34.805705559207496 38.0 34.0 40.0 26.0 41.0 60-61 34.44589059461325 37.5 34.0 40.0 26.0 41.0 62-63 34.071093182097364 37.0 33.5 40.0 25.5 41.0 64-65 33.6917863405953 36.0 33.0 39.0 25.0 41.0 66-67 33.291961861378866 36.0 33.0 39.0 23.5 40.0 68-69 32.84961899683917 35.0 32.0 38.5 22.5 40.0 70-71 32.369688091224134 35.0 32.0 38.0 22.0 40.0 72-73 32.00931517174972 35.0 31.0 37.0 20.5 39.5 74-75 31.586357524366633 35.0 31.0 37.0 20.0 39.0 76-77 29.54539313529476 33.0 28.5 35.0 16.0 37.0 78-79 30.617830626516515 34.0 30.5 36.0 18.0 37.5 80-81 30.68104144386263 34.0 31.0 35.5 18.0 37.0 82-83 30.380738877996656 34.0 31.0 35.0 15.0 37.0 84-85 29.998805718987988 34.0 30.0 35.0 9.0 36.0 86-87 29.474214427266855 34.0 29.5 35.0 4.5 36.0 88-89 28.95990000620806 34.0 29.0 35.0 2.0 35.5 90-91 28.540942484747326 34.0 29.0 35.0 2.0 35.0 92-93 28.258215442570823 34.0 29.0 35.0 2.0 35.0 94-95 27.86395367114278 33.5 27.5 35.0 2.0 35.0 96-97 27.330852934585103 33.0 27.0 35.0 2.0 35.0 98-99 26.57862771938337 33.0 25.5 35.0 2.0 35.0 100-101 23.36090885995806 29.5 12.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03798783225188984 1101 2 0.05013604585030862 1101 3 0.10621354527439308 1101 4 0.10218393804321124 1101 5 0.07327225424899098 1101 6 0.1003782113587448 1101 7 0.07189556980168277 1101 8 0.09865718819565927 1101 9 0.14572710893085628 1101 10-11 0.1660511263354607 1101 12-13 0.17038466078841452 1101 14-15 0.13606341068057048 1101 16-17 0.17341217198223546 1101 18-19 0.17256495981039421 1101 20-21 0.16699355479179445 1101 22-23 0.18873120504814977 1101 24-25 0.11621043177400736 1101 26-27 0.20929174082864677 1101 28-29 0.24519641418988414 1101 30-31 0.22472859808118528 1101 32-33 0.1924303086069088 1101 34-35 0.26521697803621436 1101 36-37 0.28110492974105483 1101 38-39 0.17203182114651128 1101 40-41 0.33513407512998583 1101 42-43 0.2931984796358975 1101 44-45 0.2791091744104861 1101 46-47 0.2984568176325979 1101 48-49 0.2360698844489022 1101 50-51 0.25275545543107825 1101 52-53 0.28636617716318113 1101 54-55 0.29780993190301785 1101 56-57 0.13871725301858362 1101 58-59 0.1276623499263323 1101 60-61 0.15963804533964066 1101 62-63 0.1185097129621866 1101 64-65 0.17110322060714367 1101 66-67 0.07766290667641584 1101 68-69 0.1277594656269585 1101 70-71 0.1336255535653592 1101 72-73 0.15969787679321712 1101 74-75 0.16024538199371108 1101 76-77 0.07194167614174418 1101 78-79 0.16705288807089858 1101 80-81 0.24659462937840004 1101 82-83 0.32765456705817897 1101 84-85 0.2761497206417687 1101 86-87 0.3371605189536204 1101 88-89 0.42320461377590135 1101 90-91 0.5062651296285559 1101 92-93 0.44734920692775404 1101 94-95 0.36558984545760254 1101 96-97 0.17517174602449614 1101 98-99 0.2768301771759134 1101 100-101 0.8085407815712102 1102 1 0.07900815010933471 1102 2 0.12164441744230459 1102 3 0.09728553295878584 1102 4 0.08636552505824113 1102 5 0.025714620661645426 1102 6 0.06480059399460458 1102 7 0.12792101102544962 1102 8 0.09560009571097794 1102 9 0.14006222454695916 1102 10-11 0.23148075126267287 1102 12-13 0.1791819278279121 1102 14-15 0.218961523555798 1102 16-17 0.23461472113087467 1102 18-19 0.19755231365789427 1102 20-21 0.1873215374690389 1102 22-23 0.20521949285723196 1102 24-25 0.1167280387179872 1102 26-27 0.13787402898729084 1102 28-29 0.07731909022894712 1102 30-31 0.07168909753436736 1102 32-33 0.16226932575967368 1102 34-35 0.1885215290622284 1102 36-37 0.1565064989543714 1102 38-39 0.16089264035965556 1102 40-41 0.1886493887872831 1102 42-43 0.2448897097640952 1102 44-45 0.11648147059766245 1102 46-47 -0.11946336980149397 1102 48-49 0.00893639206811514 1102 50-51 0.07275537546772881 1102 52-53 0.1061018027377898 1102 54-55 0.12338960650617281 1102 56-57 -0.05726895527597975 1102 58-59 -0.14895651596277304 1102 60-61 -0.1671040316160486 1102 62-63 -0.29413104980097415 1102 64-65 -0.19088872781005506 1102 66-67 -0.07862936291307676 1102 68-69 -0.0846462827620158 1102 70-71 -0.07707500226302955 1102 72-73 -0.03883791630980227 1102 74-75 -0.05469787881424537 1102 76-77 -0.1935509381001843 1102 78-79 -0.2075432580485277 1102 80-81 -0.12095890011577026 1102 82-83 0.050284071604199454 1102 84-85 0.036287438306953135 1102 86-87 -0.005684645719483683 1102 88-89 0.22604299610271283 1102 90-91 0.36671407089817265 1102 92-93 0.34319143445340217 1102 94-95 0.3423843503343811 1102 96-97 0.4985363059129462 1102 98-99 0.6822910875057318 1102 100-101 0.9568166730042371 1103 1 0.0422649215340094 1103 2 0.03570040743484526 1103 3 -0.03842883899501004 1103 4 0.010036502562975613 1103 5 0.020108097472402164 1103 6 0.01198334938490575 1103 7 0.07920182359796257 1103 8 0.10483308290012872 1103 9 0.05475184177092274 1103 10-11 -0.020198026676219172 1103 12-13 0.054933084153560685 1103 14-15 -0.022586939522490468 1103 16-17 -0.041486064980396975 1103 18-19 0.03508923962170485 1103 20-21 0.05729504254429685 1103 22-23 -0.08947401380572018 1103 24-25 0.0372052821058233 1103 26-27 0.009057296751329602 1103 28-29 0.07497019242061498 1103 30-31 0.06960592105762942 1103 32-33 0.1008762205338698 1103 34-35 0.018134694662940376 1103 36-37 0.008552079075393237 1103 38-39 0.12479798843153844 1103 40-41 0.11148112629854268 1103 42-43 0.1892377242873593 1103 44-45 0.1201265371634932 1103 46-47 0.04858568169304789 1103 48-49 0.09067339811104347 1103 50-51 0.26123957880610504 1103 52-53 0.17315998519040932 1103 54-55 -0.01963202433984179 1103 56-57 0.05656120785712915 1103 58-59 -0.1823457640838413 1103 60-61 -0.10421018122534775 1103 62-63 -0.08260787947603632 1103 64-65 -0.033110779954959924 1103 66-67 -0.20417919439403676 1103 68-69 -0.09835075162171592 1103 70-71 -0.11396034096937768 1103 72-73 -0.07341218415245265 1103 74-75 -0.08020875890578694 1103 76-77 -0.04288479265081335 1103 78-79 -0.0472668664094833 1103 80-81 0.024115749286746535 1103 82-83 -1.6748468414817808E-5 1103 84-85 -0.067624499944138 1103 86-87 0.1109067289184189 1103 88-89 0.16909533413693367 1103 90-91 0.14900324151546585 1103 92-93 0.13090874571625122 1103 94-95 0.005687441630023216 1103 96-97 0.13597939514520618 1103 98-99 0.3211451217726946 1103 100-101 0.9311692272790388 1104 1 -0.064315171999759 1104 2 0.005417658712083551 1104 3 -0.02258361173408474 1104 4 -0.0044335163211215445 1104 5 -0.004912286922582609 1104 6 0.06054218413670043 1104 7 -0.01844276839509007 1104 8 0.043663339733448936 1104 9 -0.049627505694246565 1104 10-11 -0.008613237264931684 1104 12-13 -0.05108145106649431 1104 14-15 -0.02235509878543951 1104 16-17 -0.02605732055647536 1104 18-19 -5.598135968440943E-4 1104 20-21 -0.05827466331741249 1104 22-23 -0.017937928229592615 1104 24-25 -0.07160026585410861 1104 26-27 -0.021393148986923904 1104 28-29 -0.045293637128239084 1104 30-31 -0.07701011978348049 1104 32-33 -0.09656315797510473 1104 34-35 -0.04705701717384869 1104 36-37 -0.12667369263909478 1104 38-39 -0.048714647107857445 1104 40-41 -0.03819787396520269 1104 42-43 -0.02072647025912744 1104 44-45 -0.029852883714902134 1104 46-47 0.02889931070443197 1104 48-49 0.08988221483708259 1104 50-51 0.14004805101820494 1104 52-53 -0.047207485343840005 1104 54-55 0.016667080490286423 1104 56-57 0.07716080799440306 1104 58-59 0.01660832959321823 1104 60-61 -0.01190878445002852 1104 62-63 -0.12197752646066107 1104 64-65 0.007281349416963678 1104 66-67 -0.05046042925275884 1104 68-69 0.020438391329960837 1104 70-71 -0.11059843105356748 1104 72-73 -0.19004961087656724 1104 74-75 -0.22101898051086621 1104 76-77 -0.24638053004647276 1104 78-79 -0.13095448612444116 1104 80-81 -0.1128282849422213 1104 82-83 -0.14554843286191144 1104 84-85 -0.12982794484627647 1104 86-87 0.06368008214636589 1104 88-89 0.26984226933341304 1104 90-91 0.25707290340248434 1104 92-93 0.28133134374279223 1104 94-95 0.30655020176608616 1104 96-97 0.3731825937375213 1104 98-99 0.45948326962492914 1104 100-101 0.864493879158136 1105 1 -0.0379204808565774 1105 2 -0.027391077637751948 1105 3 -0.012420136078546307 1105 4 -0.0339486156014317 1105 5 0.026174681930982047 1105 6 -0.0164814073260402 1105 7 -0.004327660209355599 1105 8 0.006562002637878095 1105 9 0.002644629639029006 1105 10-11 -0.016028152276000185 1105 12-13 0.029038226337313233 1105 14-15 0.026513835228364258 1105 16-17 -0.042162197865863504 1105 18-19 -0.07071753122027502 1105 20-21 -0.0015525572927614917 1105 22-23 -0.02931027040065004 1105 24-25 -0.03288794543426121 1105 26-27 -0.100861235520874 1105 28-29 5.023894636266846E-4 1105 30-31 -0.048474846652084125 1105 32-33 0.0372706788810504 1105 34-35 0.053906248533102996 1105 36-37 -0.020220625175575435 1105 38-39 0.028665619066934767 1105 40-41 0.0595589028543273 1105 42-43 0.0041870067688236645 1105 44-45 0.0631062446263897 1105 46-47 0.14822057351895523 1105 48-49 0.09232114401390135 1105 50-51 0.11676835460800561 1105 52-53 0.1334253827722094 1105 54-55 0.19497919937900576 1105 56-57 0.07281742799294477 1105 58-59 0.10364283020897602 1105 60-61 0.0926479141724954 1105 62-63 0.09125821615774754 1105 64-65 0.12789855068398026 1105 66-67 0.15037289950586796 1105 68-69 0.20804875615375096 1105 70-71 0.26675449361807324 1105 72-73 0.017717144905404325 1105 74-75 0.032885608774925856 1105 76-77 -0.03603813757192498 1105 78-79 -0.07112561984070709 1105 80-81 0.022086326613248275 1105 82-83 -0.16693115047008078 1105 84-85 -0.016569542175624008 1105 86-87 -0.010725022861475253 1105 88-89 0.1017628566770874 1105 90-91 0.20075238047069632 1105 92-93 0.22592317002101225 1105 94-95 0.15935303369315434 1105 96-97 0.09361473282815425 1105 98-99 0.14104388846699223 1105 100-101 0.6224035488093413 1106 1 -0.039803167386253335 1106 2 -0.06192853331793202 1106 3 -0.021896969010946066 1106 4 0.04877969787855818 1106 5 0.03437143293813705 1106 6 -0.0797240963675705 1106 7 -0.02307361466741753 1106 8 -0.049539399172594756 1106 9 -0.012161450025544696 1106 10-11 0.006728323006335302 1106 12-13 0.03931631809744829 1106 14-15 0.11589163087898413 1106 16-17 -0.044609448829547205 1106 18-19 -0.08159460471146218 1106 20-21 -0.012710101564273657 1106 22-23 -0.1104852769218212 1106 24-25 -0.14183469161091722 1106 26-27 -0.046989410365576134 1106 28-29 -0.019944202137104128 1106 30-31 -0.017318739453671128 1106 32-33 0.025808209433954232 1106 34-35 -0.08294356645883028 1106 36-37 -0.008415794194696957 1106 38-39 0.030106918857292442 1106 40-41 0.050617651368952465 1106 42-43 0.043397063382265344 1106 44-45 0.1489372472445254 1106 46-47 0.06960422131769661 1106 48-49 0.07594998305205536 1106 50-51 0.07534623189403789 1106 52-53 0.1581762498465693 1106 54-55 0.1496335048017272 1106 56-57 0.06823992748574881 1106 58-59 0.16422693400671307 1106 60-61 0.03170876878417772 1106 62-63 0.0724238032081459 1106 64-65 0.036949333707674725 1106 66-67 -0.01594899037809938 1106 68-69 0.0477909524845046 1106 70-71 -0.05264738206423658 1106 72-73 0.036870068805740175 1106 74-75 0.14676670848606221 1106 76-77 0.14432419807038244 1106 78-79 0.19718317456364076 1106 80-81 0.0715578701505244 1106 82-83 0.23512446788194552 1106 84-85 0.08950034063649781 1106 86-87 0.20176918054112747 1106 88-89 0.3198006601531098 1106 90-91 0.22955814856815593 1106 92-93 0.38602428441686243 1106 94-95 0.4135613030223908 1106 96-97 0.36162464017920826 1106 98-99 0.3931367016523204 1106 100-101 0.8240227920635554 1107 1 -0.07988964747724125 1107 2 -0.026759663703842307 1107 3 -0.14620322014064158 1107 4 -0.038212947386924156 1107 5 -0.10445850480945751 1107 6 -0.06966476241384356 1107 7 -0.07189157275379898 1107 8 -0.015457055823780763 1107 9 -0.04949873500927282 1107 10-11 -0.03519506478108525 1107 12-13 -0.06925244488166982 1107 14-15 -0.06193142828171716 1107 16-17 -0.05192111695247803 1107 18-19 -0.02211255782523125 1107 20-21 -0.08298373450950436 1107 22-23 -0.12373707416699631 1107 24-25 -0.1657167362476386 1107 26-27 -0.07944413490243818 1107 28-29 -0.04513456745856104 1107 30-31 -0.17198477901832376 1107 32-33 0.01946084878753851 1107 34-35 0.05613806059661641 1107 36-37 0.03586122407532599 1107 38-39 0.025612375315738234 1107 40-41 0.004040231303740427 1107 42-43 -0.08065115934196143 1107 44-45 -0.13241210639444745 1107 46-47 -0.16587071078242843 1107 48-49 -0.256265745171639 1107 50-51 -0.07209136178646958 1107 52-53 -0.18133787632682896 1107 54-55 -0.08212868935284234 1107 56-57 -0.02177668933637733 1107 58-59 -0.010312941235895323 1107 60-61 0.006665041343460132 1107 62-63 -0.08302125472281574 1107 64-65 0.09956851860923877 1107 66-67 0.05810207877360796 1107 68-69 0.08151120263072897 1107 70-71 0.012091651346210597 1107 72-73 0.050371113449564575 1107 74-75 0.04871371915508149 1107 76-77 0.03648139743041767 1107 78-79 0.03486261295807225 1107 80-81 0.07411866826314295 1107 82-83 0.20419835383857077 1107 84-85 0.0432124471388633 1107 86-87 0.041366972936813795 1107 88-89 0.19023542457349762 1107 90-91 0.3868790860797482 1107 92-93 0.40269283061363836 1107 94-95 0.5008422169022246 1107 96-97 0.5727908348420918 1107 98-99 0.44253706741578114 1107 100-101 0.9135291978573612 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9 3473.0 10 5659.0 11 8561.0 12 11813.0 13 16115.0 14 19485.0 15 25423.0 16 33032.0 17 43380.0 18 40193.0 19 27722.0 20 25542.0 21 26719.0 22 28853.0 23 31689.0 24 37272.0 25 44708.0 26 53976.0 27 65963.0 28 80413.0 29 97390.0 30 118988.0 31 143804.0 32 176309.0 33 223423.0 34 293631.0 35 412158.0 36 618776.0 37 959171.0 38 790486.0 39 74925.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.76651114768764 19.92135359853474 14.824806897758794 22.487328356018832 2 15.712863584269568 21.600812067059376 39.10131069314088 23.585013655530172 3 19.512350296599966 25.834951024572028 29.782000236874907 24.8706984419531 4 13.078400750955094 17.27357422660123 36.93313479282912 32.714890229614554 5 13.488815145552586 38.1568490530067 33.66544985758887 14.688885943851842 6 31.027838965569927 36.653926201578784 17.37985973307104 14.938375099780252 7 28.15518036174937 33.27526669340682 20.78751220147928 17.78204074336453 8 26.16628970681667 34.62739092857165 19.907024397675105 19.29929496693658 9 26.834690814404986 16.90685840912523 20.559531110965327 35.698919665504455 10-11 25.011656622965155 26.854911037622166 26.8766282398037 21.25680409960898 12-13 26.3346227899197 24.411070863902157 27.45649845172749 21.797807894450656 14-15 23.171790951247576 25.46880040964484 26.476137494753683 24.883271144353905 16-17 21.86681355013174 28.796512134121866 27.018962430789255 22.317711884957138 18-19 21.67720717829095 27.291460556516117 29.69408300410745 21.33724926108548 20-21 22.848956330252122 26.243995296843288 29.385043107500064 21.522005265404523 22-23 22.43083186680762 26.17768215702341 29.275019691877702 22.116466284291274 24-25 22.338547516533914 26.43518455329047 29.425642192968514 21.800625737207106 26-27 22.097203563473897 26.472602230831082 29.690119543795205 21.74007466189981 28-29 21.800493650827896 26.650034056271437 29.776551673732556 21.77292061916811 30-31 21.922860673966348 26.609791739405903 29.473611563017748 21.99373602361 32-33 21.723179090197824 26.746501141886746 29.384431242655445 22.14588852525998 34-35 22.390900759829485 26.534830792771025 29.17104557050508 21.90322287689441 36-37 22.37866423234958 26.317794855721687 29.24436978853081 22.059171123397924 38-39 22.223937095040185 26.606326383548257 29.42163993850236 21.748096582909195 40-41 22.0686279873535 26.427555463283554 29.580979305618193 21.922837243744752 42-43 21.928522672822698 26.737682482404644 28.935061069543405 22.398733775229253 44-45 22.11740722680436 26.80028927471081 28.685715355005453 22.396588143479377 46-47 22.18579028058221 26.94725841689393 28.329277485177478 22.53767381734638 48-49 22.432232250147855 26.968277141029635 28.071660831199864 22.52782977762265 50-51 22.244822548303382 27.17096226352166 28.147393214009632 22.436821974165326 52-53 22.43901021512028 27.30157213611413 27.870688315330693 22.388729333434892 54-55 22.00553574015263 26.981053529766324 28.174707131475703 22.838703598605345 56-57 22.238183882731953 27.40845203129038 27.745272298272177 22.608091787705487 58-59 22.43704099067917 26.911841481470557 27.836924027611033 22.814193500239245 60-61 22.37060150682985 26.964575618380433 28.217093394853727 22.447729479935997 62-63 22.351348888118874 26.9847739627807 28.136886398228988 22.52699075087144 64-65 22.077522816821286 26.936166175231957 28.10747516445855 22.878835843488208 66-67 21.978699715075255 27.225354724979102 27.780377342291096 23.015568217654547 68-69 21.947270044897614 27.165350213810996 27.95162254525177 22.935757196039624 70-71 22.07817453328637 27.056853205354 27.811421275096865 23.05355098626277 72-73 22.129069526307426 26.9714112240841 27.663841045490106 23.235678204118365 74-75 21.949178885023652 27.454583199228967 27.399327063926727 23.196910851820657 76-77 22.089218045548535 26.995339020353594 27.78865201357119 23.126790920526677 78-79 21.842576046350633 27.087512281524305 27.82390273396118 23.246008938163882 80-81 21.805200924913162 27.449837153951457 27.51615554541181 23.228806375723572 82-83 21.991479547965188 27.25959068192235 27.433130169805846 23.315799600306615 84-85 21.71731225352131 27.0872840002448 27.39641015638587 23.798993589848017 86-87 21.76044364843203 27.141045626510873 27.62473450686983 23.47377621818726 88-89 21.865423061598957 26.950239012112714 27.410936957518476 23.77340096876985 90-91 21.62732043082914 27.009947305331057 27.668012547821462 23.69471971601834 92-93 21.77423047576193 27.23381182358015 27.46765975077061 23.524297949887305 94-95 21.57162572611751 26.987014365057334 27.40610252414925 24.035257384675905 96-97 21.60671430362457 26.803653465622258 27.44499895046353 24.14463328028964 98-99 21.482642050407797 26.806901616564726 27.259320475723353 24.45113585730413 100-101 21.546677873536144 26.49785842536146 27.766050142081856 24.189413559020547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4082.0 1 2647.5 2 1511.0 3 1844.5 4 1626.0 5 1633.0 6 2885.5 7 5869.0 8 8822.5 9 9470.5 10 7841.0 11 6231.5 12 6053.5 13 6241.5 14 6557.0 15 6807.0 16 6942.5 17 7160.0 18 7510.5 19 8014.5 20 8829.5 21 10013.5 22 11430.5 23 13492.0 24 15694.5 25 18981.0 26 24678.5 27 30601.0 28 35389.0 29 41210.5 30 49334.0 31 59026.5 32 69200.5 33 78189.5 34 87333.5 35 95578.5 36 106808.0 37 118319.5 38 123187.0 39 124623.0 40 124212.5 41 128031.0 42 133419.5 43 139109.5 44 145623.5 45 150278.5 46 152865.0 47 162971.0 48 200188.5 49 204831.5 50 175780.0 51 163851.5 52 156140.0 53 155717.5 54 153150.5 55 141589.5 56 129019.0 57 116970.5 58 107694.5 59 98092.5 60 84868.0 61 69225.0 62 54332.0 63 41451.5 64 29899.0 65 22246.0 66 17082.0 67 13710.0 68 10326.0 69 7333.0 70 5539.0 71 4227.0 72 3272.5 73 2877.5 74 2361.5 75 1744.0 76 1191.0 77 645.5 78 364.5 79 241.0 80 145.5 81 90.5 82 60.0 83 34.5 84 20.0 85 11.5 86 6.5 87 3.5 88 3.0 89 2.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3834467588424126 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.3115116361495762E-4 16-17 0.002322518834416955 18-19 1.7611517227806295E-4 20-21 2.201439653475787E-5 22-23 0.0 24-25 0.0 26-27 9.90647844064104E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007583959606224087 36-37 0.016653890978544326 38-39 0.011425471801539334 40-41 0.007826117968106424 42-43 0.0035333106438286384 44-45 0.001915252498523935 46-47 0.001541007757433051 48-49 1.1007198267378935E-5 50-51 4.5129512896253634E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.95323922032052E-4 60-61 6.934534908448729E-4 62-63 0.0 64-65 0.0 66-67 0.001199784611144304 68-69 7.815110769839043E-4 70-71 0.0018051805158501453 72-73 0.0 74-75 0.0 76-77 0.09878960444972594 78-79 0.03558627199843609 80-81 3.3021594802136805E-5 82-83 0.0 84-85 0.0 86-87 0.15010516277224653 88-89 0.323127312337176 90-91 0.2776455690963663 92-93 0.0 94-95 2.201439653475787E-5 96-97 1.1007198267378935E-5 98-99 2.2014396534757869E-4 100-101 6.604318960427361E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4542482.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.42177117054655 #Duplication Level Percentage of deduplicated Percentage of total 1 63.39615039018044 21.18811631434166 2 15.23628968925695 10.18447574965007 3 6.966113594420259 6.984595635022421 4 3.998423831259501 5.345376253248603 5 2.414328896493087 4.034557395451506 6 1.6502674097313441 3.309291584295094 7 1.1821358803907027 2.7656352420837456 8 0.8348032911478778 2.2320483655330814 9 0.6253619536522236 1.8810633702357498 >10 3.2751230771560604 19.479503746967332 >50 0.24344599166904127 5.707453574899304 >100 0.1624542631961806 10.231729083719555 >500 0.010485481834350836 2.3968563275993926 >1k 0.004154624650809315 2.4667465337091534 >5k 2.6378569211487506E-4 0.6190284325785164 >10k+ 1.978392690861563E-4 1.1735223906647627 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22620 0.497965649616223 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17508 0.3854280545305408 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13116 0.28874082494988423 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9293 0.2045797869975049 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7401 0.162928548753743 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6352 0.139835446788782 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5040 0.11095255853517966 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4549 0.10014348983661354 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.402879306951574E-5 0.0 2 0.0 0.0 0.0 8.805758613903148E-5 0.0 3 0.0 0.0 0.0 1.7611517227806295E-4 0.0 4 0.0 0.0 0.0 1.7611517227806295E-4 0.0 5 0.0 0.0 0.0 1.9812956881282083E-4 0.0 6 0.0 0.0 0.0 3.3021594802136803E-4 0.0 7 0.0 0.0 0.0 3.3021594802136803E-4 0.0 8 0.0 0.0 0.0 3.522303445561259E-4 0.0 9 0.0 0.0 0.0 6.164031029732204E-4 0.0 10-11 0.0 0.0 0.0 8.695686631229359E-4 0.0 12-13 0.0 0.0 0.0 0.0010787054302031356 0.0 14-15 0.0 0.0 0.0 0.0011227342232726515 0.0 16-17 0.0 0.0 0.0 0.0012328062059464408 0.0 18-19 0.0 0.0 0.0 0.0017941733175827665 0.0 20-21 0.0 0.0 0.0 0.002366547627486471 0.0 22-23 0.0 0.0 0.0 0.003225109092342028 0.0 24-25 0.0 0.0 0.0 0.004072663358930206 0.0 26-27 0.0 0.0 0.0 0.0054925919354220885 0.0 28-29 0.0 0.0 0.0 0.009367125725539473 0.0 30-31 0.0 0.0 0.0 0.021353964638715132 0.0 32-33 0.0 0.0 0.0 0.044381023414071866 0.0 34-35 0.0 0.0 0.0 0.08127715200632606 0.0 36-37 1.1007198267378935E-5 0.0 0.0 0.13907595010833285 0.0 38-39 2.201439653475787E-5 0.0 0.0 0.22533936292978157 0.0 40-41 2.201439653475787E-5 0.0 0.0 0.32096990147676974 0.0 42-43 2.201439653475787E-5 0.0 0.0 0.4176131022643568 0.0 44-45 2.201439653475787E-5 0.0 0.0 0.5344875334673864 0.0 46-47 2.201439653475787E-5 0.0 0.0 0.6766234846940505 0.0 48-49 2.201439653475787E-5 0.0 0.0 0.8341805206933126 0.0 50-51 2.201439653475787E-5 0.0 0.0 1.0027667693564883 0.0 52-53 2.201439653475787E-5 0.0 0.0 1.16855718965975 0.0 54-55 2.201439653475787E-5 0.0 0.0 1.349207327623973 0.0 56-57 2.201439653475787E-5 0.0 0.0 1.5648933776732632 0.0 58-59 2.201439653475787E-5 0.0 0.0 1.8064573508491613 0.0 60-61 2.201439653475787E-5 0.0 0.0 2.080338458138084 0.0 62-63 2.201439653475787E-5 0.0 0.0 2.350223952455948 0.0 64-65 2.201439653475787E-5 0.0 0.0 2.6215293753503044 0.0 66-67 2.201439653475787E-5 0.0 0.0 2.9337705686010427 0.0 68-69 2.201439653475787E-5 0.0 0.0 3.2867604098376173 0.0 70-71 2.201439653475787E-5 0.0 0.0 3.6755566670379762 0.0 72-73 2.201439653475787E-5 0.0 0.0 4.072079977422035 0.0 74-75 2.201439653475787E-5 0.0 0.0 4.47915919094451 0.0 76-77 2.201439653475787E-5 0.0 0.0 4.905060713504203 0.0 78-79 2.201439653475787E-5 0.0 0.0 5.381221103352749 0.0 80-81 2.201439653475787E-5 0.0 0.0 5.902588056485419 0.0 82-83 2.201439653475787E-5 0.0 0.0 6.43533645262656 0.0 84-85 2.201439653475787E-5 0.0 0.0 6.974755210917731 0.0 86-87 2.201439653475787E-5 0.0 0.0 7.534063976478057 0.0 88-89 2.201439653475787E-5 0.0 0.0 8.119955125854105 2.201439653475787E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 25955 0.0 24.49787 1 TACATGG 26810 0.0 23.327742 2 ACATGGG 26710 0.0 22.171484 3 GTATCAA 40645 0.0 22.08881 1 GAGTACT 19595 0.0 21.663202 12-13 AGTACTT 20430 0.0 20.464216 12-13 GTACTTT 20990 0.0 20.313894 14-15 GGTATCA 31415 0.0 20.285566 1 CATGGGA 16175 0.0 20.154245 4 ATCAACG 47660 0.0 18.678108 3 TCAACGC 47940 0.0 18.559118 4 TATCAAC 48255 0.0 18.506804 2 CAACGCA 48240 0.0 18.4437 5 CATGGGG 9925 0.0 18.215813 4 AACGCAG 48980 0.0 18.213491 6 ATGGGAG 5895 0.0 17.950441 5 ACTTTTT 24300 0.0 17.712465 16-17 TACTTTT 23515 0.0 17.496923 14-15 ATGGGAT 7380 0.0 17.03898 5 CGTACAC 390 3.0559022E-10 17.034035 3 >>END_MODULE