FastQCFastQC Report
Thu 26 May 2016
SRR938720_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938720_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4545886
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239090.5259480770085303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167150.36769509838126163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121110.26641671172572295No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86730.19078789041344194No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA76390.16804204944866633No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA61350.13495718986353816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47640.10479805256885016No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47240.10391813609052229No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG262000.027.2090681
TACATGG268750.026.4247462
ACATGGG269600.025.00443
GTATCAA395600.023.798631
GAGTACT179900.022.80899212-13
AGTACTT187450.021.68773312-13
CATGGGA164200.021.6685754
GTACTTT190850.021.5510214-15
CATGGGG98150.020.7352494
TATCAAC460050.020.3347742
ATCAACG459650.020.301093
TCAACGC461900.020.212474
CAACGCA464400.020.0525865
AACGCAG469850.019.8695936
GGTATCA296150.019.433731
GTATAGG13650.018.8011681
ACTTTTT226650.018.59755316-17
TACTTTT218550.018.36344714-15
ATGGGAT76750.018.3577865
ACGCAGA509150.018.2716987
Produced by FastQC (version 0.11.5)