##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938720_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4545886 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.598553285322158 33.0 31.0 34.0 30.0 34.0 2 31.653282550420315 33.0 31.0 34.0 30.0 34.0 3 31.66765576611468 33.0 31.0 34.0 28.0 34.0 4 35.22819995925987 37.0 35.0 37.0 33.0 37.0 5 35.26100720519608 37.0 35.0 37.0 33.0 37.0 6 35.32931776115811 37.0 35.0 37.0 33.0 37.0 7 35.24052780910036 37.0 35.0 37.0 32.0 37.0 8 35.2074796860282 37.0 35.0 37.0 32.0 37.0 9 36.779776703595296 39.0 37.0 39.0 33.0 39.0 10-11 36.66215078864714 39.0 37.0 39.0 32.0 39.0 12-13 36.54589006411511 39.0 37.0 39.0 32.0 39.0 14-15 37.685299301390316 40.0 37.0 41.0 32.0 41.0 16-17 37.573605123401684 40.0 37.0 41.0 32.0 41.0 18-19 37.53179424209054 40.0 37.0 41.0 32.0 41.0 20-21 37.431261584650386 40.0 37.0 41.0 32.0 41.0 22-23 37.447424550461676 40.0 37.0 41.0 32.0 41.0 24-25 37.378376404511684 40.0 37.0 41.0 31.5 41.0 26-27 37.23797319158466 40.0 37.0 41.0 31.0 41.0 28-29 37.06866604221928 40.0 36.5 41.0 30.5 41.0 30-31 36.833566877831956 39.0 36.0 41.0 30.0 41.0 32-33 36.64209474236706 39.0 35.5 41.0 30.0 41.0 34-35 36.34881198076678 39.0 35.0 41.0 30.0 41.0 36-37 36.132669846978125 39.0 35.0 41.0 29.5 41.0 38-39 35.95552726135236 39.0 35.0 41.0 29.0 41.0 40-41 35.835577047026696 39.0 35.0 40.5 28.0 41.0 42-43 35.814120393692235 39.0 35.0 41.0 27.5 41.0 44-45 35.61513256161725 39.0 35.0 41.0 27.0 41.0 46-47 35.278534811475694 39.0 35.0 41.0 25.5 41.0 48-49 34.97714966895342 39.0 35.0 41.0 24.0 41.0 50-51 33.83527325586255 38.0 33.0 39.5 23.0 40.5 52-53 33.90850089509504 38.0 33.5 39.5 23.0 40.5 54-55 34.40137148181894 38.0 34.0 40.0 23.0 41.0 56-57 34.28921809741819 38.0 34.0 40.0 23.0 41.0 58-59 34.07915904622333 38.0 34.0 40.0 22.0 41.0 60-61 33.75723852291941 37.5 33.5 40.0 21.5 41.0 62-63 33.35510096381651 37.0 33.0 40.0 19.5 41.0 64-65 32.94819161765165 36.5 32.5 39.5 18.0 41.0 66-67 32.551743158539395 36.0 32.0 39.0 16.5 41.0 68-69 32.12597533242145 35.0 31.5 39.0 13.0 40.5 70-71 31.6968210377471 35.0 31.0 38.0 9.5 40.0 72-73 31.2703651169431 35.0 31.0 37.5 7.5 39.5 74-75 30.79808633564502 35.0 30.5 37.0 4.5 39.0 76-77 30.33837463147998 35.0 30.0 36.0 2.0 39.0 78-79 29.906543960847237 34.0 30.0 36.0 2.0 38.5 80-81 29.443988696592918 34.0 29.0 35.0 2.0 37.0 82-83 29.03370443077543 34.0 29.0 35.0 2.0 37.0 84-85 28.672637523246294 34.0 29.0 35.0 2.0 36.0 86-87 28.251354521428823 34.0 28.5 35.0 2.0 36.0 88-89 27.791249274618853 34.0 27.0 35.0 2.0 36.0 90-91 27.323636800394908 34.0 26.0 35.0 2.0 35.0 92-93 26.853986549596712 33.0 25.0 35.0 2.0 35.0 94-95 26.325280924334663 33.0 24.5 35.0 2.0 35.0 96-97 25.7010500263315 33.0 22.0 35.0 2.0 35.0 98-99 24.905898762089503 32.0 10.5 35.0 2.0 35.0 100-101 22.36772325570857 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10842595376162478 1101 2 0.2395630864877667 1101 3 0.3713935099289323 1101 4 0.20721084277277413 1101 5 0.13765283038487297 1101 6 0.27804707763615966 1101 7 0.3541626013842176 1101 8 0.3956075316721197 1101 9 0.41513280752401016 1101 10-11 0.3650328518851609 1101 12-13 0.3223740057022155 1101 14-15 0.3442672779952929 1101 16-17 0.4788544253106579 1101 18-19 0.49723706770928544 1101 20-21 0.5943038231938402 1101 22-23 0.5032852513066501 1101 24-25 0.5427036931901412 1101 26-27 0.44800528291956 1101 28-29 0.491700373620823 1101 30-31 0.5837779506934098 1101 32-33 0.5089505392258076 1101 34-35 0.5227113975696938 1101 36-37 0.5480019588502572 1101 38-39 0.544488957046461 1101 40-41 0.5872571381641905 1101 42-43 0.7297467559702966 1101 44-45 0.7534500949028242 1101 46-47 0.6801282053026441 1101 48-49 0.6832036162725146 1101 50-51 0.6655342168599816 1101 52-53 0.7008388162333858 1101 54-55 0.7235659965541501 1101 56-57 0.656919969609838 1101 58-59 0.6517849476035522 1101 60-61 0.5760559488677828 1101 62-63 0.524380846015319 1101 64-65 0.5114632859792749 1101 66-67 0.4022227552021036 1101 68-69 0.4860989875223751 1101 70-71 0.445181632054112 1101 72-73 0.5488704761382479 1101 74-75 0.3393398642925156 1101 76-77 0.19139539952854534 1101 78-79 0.2631537746256427 1101 80-81 0.22631301747389898 1101 82-83 0.21831215832835937 1101 84-85 0.20005572594727994 1101 86-87 0.05045972720482084 1101 88-89 0.16189346728761578 1101 90-91 0.1345690597555489 1101 92-93 0.1978987357446762 1101 94-95 0.11373571630211643 1101 96-97 0.08153228234316856 1101 98-99 0.1583275606974226 1101 100-101 0.4563192755124952 1102 1 0.16364085380411098 1102 2 0.17655999762260777 1102 3 0.170149351788222 1102 4 0.28784285443926905 1102 5 0.3073193707410695 1102 6 0.29391913661167024 1102 7 0.2218121931717434 1102 8 0.29172003985358685 1102 9 0.3836384398227466 1102 10-11 0.352008519895314 1102 12-13 0.37934433422426395 1102 14-15 0.431995641585722 1102 16-17 0.5511470751895047 1102 18-19 0.5274855548543158 1102 20-21 0.4973562305565906 1102 22-23 0.46536053234745367 1102 24-25 0.4140751378374503 1102 26-27 0.4109116294465949 1102 28-29 0.504569688214275 1102 30-31 0.582764596231776 1102 32-33 0.48259901718463283 1102 34-35 0.5209286975111311 1102 36-37 0.5569456014145828 1102 38-39 0.4701566801279782 1102 40-41 0.6084141420798161 1102 42-43 0.6396213641207638 1102 44-45 0.578748359856256 1102 46-47 0.5965810053761302 1102 48-49 0.5188306329340122 1102 50-51 0.5837065617564718 1102 52-53 0.4120988205968388 1102 54-55 0.33601676417732307 1102 56-57 0.46570601115450216 1102 58-59 0.3491637182581826 1102 60-61 0.3799374122320103 1102 62-63 0.2742775010843914 1102 64-65 0.11048208327393638 1102 66-67 0.16448635367260067 1102 68-69 0.2275026987536677 1102 70-71 0.1769570405536598 1102 72-73 0.11314097575356996 1102 74-75 0.023177578302643553 1102 76-77 0.1178080673205244 1102 78-79 0.08866590617225256 1102 80-81 0.050353919101006284 1102 82-83 0.06549945379151723 1102 84-85 -0.004867224665325409 1102 86-87 -0.0799142092036007 1102 88-89 -0.028845391554693123 1102 90-91 -0.08208928190279252 1102 92-93 -0.22321463364455596 1102 94-95 -0.25421242750809725 1102 96-97 -0.26317760547016533 1102 98-99 -0.48559891512560327 1102 100-101 -0.1205578703301633 1103 1 0.15906951454984863 1103 2 0.32488240126754775 1103 3 0.40462849439674287 1103 4 0.34989537293341755 1103 5 0.35851102171891114 1103 6 0.32000574826829364 1103 7 0.2654317980232648 1103 8 0.3107618432871675 1103 9 0.34089251016934696 1103 10-11 0.3487331127440072 1103 12-13 0.41802487471310457 1103 14-15 0.4974320475962344 1103 16-17 0.5026476035938714 1103 18-19 0.5067001920049918 1103 20-21 0.5107792176625949 1103 22-23 0.5200002258962471 1103 24-25 0.5199158140084066 1103 26-27 0.5684539892794689 1103 28-29 0.5202278957453785 1103 30-31 0.6468904894451057 1103 32-33 0.6301156916665533 1103 34-35 0.6967199626254796 1103 36-37 0.7291759693846487 1103 38-39 0.6263707387167017 1103 40-41 0.7525043534182245 1103 42-43 0.7425502756544375 1103 44-45 0.8782728542561031 1103 46-47 0.8371114277030287 1103 48-49 0.8453130812775242 1103 50-51 0.9250137851490337 1103 52-53 0.8900038197919784 1103 54-55 0.8326007991336652 1103 56-57 0.9101830191493931 1103 58-59 0.8546676387152488 1103 60-61 0.7827792478597573 1103 62-63 0.6446143034564926 1103 64-65 0.5537130251401265 1103 66-67 0.612290092862473 1103 68-69 0.636131028692617 1103 70-71 0.549938780858664 1103 72-73 0.6638861658741604 1103 74-75 0.5348820847869717 1103 76-77 0.5391058540877864 1103 78-79 0.5225235029472373 1103 80-81 0.5138884776792381 1103 82-83 0.6130191027350591 1103 84-85 0.6683485066206458 1103 86-87 0.5712435562408551 1103 88-89 0.7315223568828415 1103 90-91 0.6055668773323859 1103 92-93 0.5088251588196719 1103 94-95 0.5353079609682574 1103 96-97 0.5255116834352087 1103 98-99 0.6052003168800688 1103 100-101 1.0649689243200022 1104 1 0.14911265211446434 1104 2 0.16495510235087707 1104 3 0.1361823287284345 1104 4 0.3148837406447882 1104 5 0.18773995480543704 1104 6 0.25723227130833237 1104 7 0.32479307613574804 1104 8 0.33314921896329963 1104 9 0.3710241922499762 1104 10-11 0.3018709002629194 1104 12-13 0.3233325015244617 1104 14-15 0.43560110595829116 1104 16-17 0.5507328060967609 1104 18-19 0.4429352034355176 1104 20-21 0.4966600913822674 1104 22-23 0.45450526226311894 1104 24-25 0.4413060382357301 1104 26-27 0.4278818117307921 1104 28-29 0.4084260804665476 1104 30-31 0.40396445243046486 1104 32-33 0.4017737794362901 1104 34-35 0.5186385302638712 1104 36-37 0.6002108116776341 1104 38-39 0.5711458791890536 1104 40-41 0.5575764472820666 1104 42-43 0.6307445211352203 1104 44-45 0.6490735567475667 1104 46-47 0.7539704445954456 1104 48-49 0.8187098776950847 1104 50-51 0.7643476144423431 1104 52-53 0.7368214612092743 1104 54-55 0.6856053058168285 1104 56-57 0.6884503115980252 1104 58-59 0.6941011500061691 1104 60-61 0.6460379095287792 1104 62-63 0.6231547149706813 1104 64-65 0.6590233325565222 1104 66-67 0.6446034655843675 1104 68-69 0.6152012199994275 1104 70-71 0.6646401172603724 1104 72-73 0.5547086293060026 1104 74-75 0.4588869317820681 1104 76-77 0.4780313186635432 1104 78-79 0.35899645890902576 1104 80-81 0.4091563018292419 1104 82-83 0.38473445360896363 1104 84-85 0.461965241397742 1104 86-87 0.42753401392101864 1104 88-89 0.45858800326703175 1104 90-91 0.4909197054860037 1104 92-93 0.3791386157068004 1104 94-95 0.6062006207030102 1104 96-97 0.6501327957652414 1104 98-99 0.8333473568099414 1104 100-101 1.2195905228099484 1105 1 0.08836451388096833 1105 2 0.13373639448765573 1105 3 0.24065129897245896 1105 4 0.23409284370900707 1105 5 0.22391393007081462 1105 6 0.26629644683631426 1105 7 0.1886442488889628 1105 8 0.2504384463958971 1105 9 0.31235704666321595 1105 10-11 0.30127571477683546 1105 12-13 0.34278841813202376 1105 14-15 0.35320321056698134 1105 16-17 0.3664572941153921 1105 18-19 0.3436889604952924 1105 20-21 0.4489950271652674 1105 22-23 0.34193952564890395 1105 24-25 0.37851541894161045 1105 26-27 0.3498571025651458 1105 28-29 0.4237552472893569 1105 30-31 0.5071672069751898 1105 32-33 0.47609998576929513 1105 34-35 0.38642090162068143 1105 36-37 0.5710701267794818 1105 38-39 0.52177687762196 1105 40-41 0.5333986189725621 1105 42-43 0.5420745873473436 1105 44-45 0.5208541802345934 1105 46-47 0.634606823808717 1105 48-49 0.7336003329732108 1105 50-51 0.7562743698364969 1105 52-53 0.7656571173757527 1105 54-55 0.7338100507553449 1105 56-57 0.6382063616762821 1105 58-59 0.6991685111069685 1105 60-61 0.642629233485934 1105 62-63 0.7371360705549463 1105 64-65 0.6224327298077768 1105 66-67 0.565190830908719 1105 68-69 0.5088630236945093 1105 70-71 0.38822660181484636 1105 72-73 0.4412072544140919 1105 74-75 0.45430183755713216 1105 76-77 0.5674522519496463 1105 78-79 0.6185558995624163 1105 80-81 0.7313474838073262 1105 82-83 0.72616332772958 1105 84-85 0.598801879916067 1105 86-87 0.5514429681947526 1105 88-89 0.6442167237655418 1105 90-91 0.7629087723436712 1105 92-93 0.7533287351987781 1105 94-95 0.8546018360098806 1105 96-97 0.7880084457660992 1105 98-99 0.685607437759689 1105 100-101 1.2035038043341224 1106 1 0.144933040731285 1106 2 0.11351214216848504 1106 3 0.15994815980003452 1106 4 0.19629232773757366 1106 5 0.23251677746873156 1106 6 0.18587718888798577 1106 7 0.2411505046617961 1106 8 0.23984012171055724 1106 9 0.21492790272435514 1106 10-11 0.24265818864245148 1106 12-13 0.23233307264857928 1106 14-15 0.4344089579846937 1106 16-17 0.43094008329688904 1106 18-19 0.44988629533526847 1106 20-21 0.508491961940436 1106 22-23 0.4010437349843059 1106 24-25 0.32662781651934125 1106 26-27 0.27976412800229156 1106 28-29 0.19460168416456014 1106 30-31 0.24489093653748029 1106 32-33 0.3252469573105472 1106 34-35 0.365840274506283 1106 36-37 0.5728898054259375 1106 38-39 0.6089317369068823 1106 40-41 0.6939167645219229 1106 42-43 0.7833696119480678 1106 44-45 0.7477917388283686 1106 46-47 0.7983786636221524 1106 48-49 0.7904156980098449 1106 50-51 0.8689885097525547 1106 52-53 0.6719297153597807 1106 54-55 0.6697033695761192 1106 56-57 0.7648360822402225 1106 58-59 0.904458741576974 1106 60-61 0.9621537532073674 1106 62-63 0.8882748496995134 1106 64-65 0.8315645656330304 1106 66-67 0.841892491129201 1106 68-69 0.8725359205154533 1106 70-71 0.9050284928792358 1106 72-73 0.9432237804556998 1106 74-75 0.8935938377842376 1106 76-77 0.8916665587324673 1106 78-79 0.886489438121103 1106 80-81 0.9502993250886433 1106 82-83 0.8643860650923685 1106 84-85 1.0546443632259255 1106 86-87 1.1276776784212679 1106 88-89 1.1029107593517828 1106 90-91 1.2149264062144418 1106 92-93 1.495357488904812 1106 94-95 1.5249899778064773 1106 96-97 1.4942289065399486 1106 98-99 1.5949997519178858 1106 100-101 1.9080523431766885 1107 1 0.06940068107279629 1107 2 0.03696686388773429 1107 3 0.11083524556041624 1107 4 0.1148819485768584 1107 5 0.12318539650421911 1107 6 -0.08059542293813848 1107 7 0.07219395086917757 1107 8 0.10748348197443391 1107 9 0.09965394460753885 1107 10-11 0.07750812665801732 1107 12-13 0.09890691268854113 1107 14-15 0.17918069759151223 1107 16-17 0.1558825315157435 1107 18-19 0.22523140143804454 1107 20-21 0.1601155595618735 1107 22-23 0.20651757813394767 1107 24-25 0.11681399896362876 1107 26-27 0.1038964959168851 1107 28-29 0.01108497073451531 1107 30-31 -0.03676977173086726 1107 32-33 0.017075059019219907 1107 34-35 0.08615357550986857 1107 36-37 0.18680000591653823 1107 38-39 0.07217183808236172 1107 40-41 0.029097446836281904 1107 42-43 0.08186677350276739 1107 44-45 0.28467525191384624 1107 46-47 0.331482280832752 1107 48-49 0.34299372138553963 1107 50-51 0.38515042652191767 1107 52-53 0.39661479677788947 1107 54-55 0.2788422596318938 1107 56-57 0.40414117096516833 1107 58-59 0.5487652296571781 1107 60-61 0.5165953104397261 1107 62-63 0.3857053188372248 1107 64-65 0.33517933940574807 1107 66-67 0.07424938545887017 1107 68-69 0.3116096837227218 1107 70-71 0.39701666118957846 1107 72-73 0.2888441621968987 1107 74-75 0.3935136862792561 1107 76-77 0.3176092504518664 1107 78-79 0.3566443001032127 1107 80-81 0.36067798571198395 1107 82-83 0.31234500679062194 1107 84-85 0.5163901821178953 1107 86-87 0.41857817762466 1107 88-89 0.4107774181624215 1107 90-91 0.5259001519820501 1107 92-93 0.5800922233391894 1107 94-95 0.5917594548040377 1107 96-97 0.579696006597576 1107 98-99 0.8185499825887348 1107 100-101 1.3383439473542786 1108 1 0.26756841855771185 1108 2 0.29119405776375373 1108 3 0.20545458998797983 1108 4 0.24250572151689198 1108 5 0.30155705338857786 1108 6 0.22342367714536948 1108 7 0.2814393274953986 1108 8 0.2999197241305609 1108 9 0.3303862197789229 1108 10-11 0.4275554238626782 1108 12-13 0.4159161537735514 1108 14-15 0.5093217428031025 1108 16-17 0.5830895406813283 1108 18-19 0.6045837923360793 1108 20-21 0.49998844422643174 1108 22-23 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13810.0 11 17059.0 12 21366.0 13 25790.0 14 28060.0 15 31729.0 16 38287.0 17 47187.0 18 41866.0 19 31105.0 20 31299.0 21 33582.0 22 36814.0 23 40844.0 24 46851.0 25 53568.0 26 61638.0 27 71559.0 28 83806.0 29 99580.0 30 118350.0 31 142967.0 32 175702.0 33 222365.0 34 293756.0 35 412829.0 36 594358.0 37 844508.0 38 708349.0 39 104634.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36095615596539 18.990199776335206 14.13956330220996 22.509280765489446 2 15.295624042317185 20.81892815324159 40.23845503314238 23.646992771298844 3 19.504942704477386 25.807593455806817 29.39568547335727 25.291778366358532 4 12.622873591685535 16.52583154783047 37.56968308682964 33.281611773654355 5 13.57273330240451 37.931033722413225 33.602240634476 14.893992340706271 6 30.968194842406877 36.16916464624201 17.859003746969364 15.003636764381751 7 28.37857892437723 33.17588833489125 20.921264931640422 17.524267809091103 8 26.181676725171165 34.13055995441311 20.176763873271444 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21.772463560170213 26.6380318689958 29.387100279317114 22.202404291516874 34-35 22.45522948888453 26.47310634359662 29.155782568841364 21.915881598677483 36-37 22.41079594306259 26.251107313870232 29.205346733073473 22.132750009993707 38-39 22.29126839590014 26.46267106896748 29.425568196560043 21.820492338572336 40-41 22.136593266950662 26.368571509926607 29.536515437161277 21.958319785961457 42-43 21.93223791009922 26.66164926436948 28.946837910452505 22.45927491507879 44-45 22.156926026101967 26.745532939701246 28.70818753941004 22.38935349478675 46-47 22.228009327631224 26.845372285398838 28.371265251283244 22.555353135686694 48-49 22.4790332662604 26.858148477827275 28.124324945167324 22.538493310744993 50-51 22.267033723179036 27.122544700364852 28.20268825569714 22.40773332075897 52-53 22.465989684929184 27.187999144827973 27.98000505328923 22.36600611695361 54-55 22.018837022454516 26.902143066317606 28.310789889924866 22.76823002130301 56-57 22.25556820872841 27.32930774755062 27.862588382841707 22.552535660879265 58-59 22.48692557719453 26.763817807616064 27.98140050707227 22.76785610811714 60-61 22.423200983493473 26.780270372435698 28.38422888511147 22.41229975895936 62-63 22.400307029572794 26.821563792769172 28.31786923596362 22.460259941694414 64-65 22.153567893454138 26.80985777199915 28.31092016902042 22.725654165526294 66-67 22.073420708537515 27.088110019504015 27.95509857931532 22.88337069264315 68-69 22.035575785772956 27.009969155398732 28.138883329113796 22.815571729714517 70-71 22.154789942736258 26.918885459038894 27.989821939164482 22.936502659060366 72-73 22.234776592586307 26.821282021375858 27.869818616224162 23.07412276981367 74-75 22.061754198093556 27.33503284830669 27.67105925241663 22.932153701183122 76-77 22.32678257837159 26.84761955347738 27.93308951908285 22.892508349068184 78-79 22.078224455315514 26.959784448991886 27.975285733589693 22.986705362102906 80-81 22.096827796062982 27.316285216807568 27.69201213190521 22.894874855224238 82-83 22.33249579158737 27.062072498982747 27.643703005598887 22.961728703830993 84-85 22.097308508959657 26.924903549576552 27.627261611842574 23.35052632962122 86-87 22.206572505575213 26.98193705435159 27.830356335435557 22.98113410463764 88-89 22.351236204245957 26.736957017694387 27.642822895126763 23.268983882932897 90-91 22.16940041778584 26.8018795392626 27.91885416274341 23.109865880208144 92-93 22.438648866858184 26.99097528945674 27.67114754424273 22.89922829944235 94-95 22.270589260602875 26.785045818468927 27.61803056523381 23.326334355694385 96-97 22.35289582352094 26.49956214261161 27.69006147676793 23.45748055709952 98-99 22.32341322526928 26.54674555271856 27.43033663831782 23.69950458369434 100-101 22.772141109186393 26.00377588883953 27.854650475199488 23.369432526774588 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7040.0 1 4557.0 2 2299.0 3 2524.0 4 2227.5 5 2264.5 6 3590.0 7 6521.0 8 9714.5 9 10445.5 10 8636.0 11 6959.0 12 6730.5 13 7064.0 14 7230.5 15 7305.5 16 7496.0 17 7818.5 18 8094.5 19 8467.5 20 9205.5 21 10286.0 22 11838.0 23 13807.5 24 15993.5 25 19371.0 26 24919.0 27 30772.0 28 35633.0 29 41297.5 30 49320.5 31 58600.0 32 68155.0 33 77378.5 34 87155.0 35 95522.5 36 106396.0 37 118177.5 38 122841.0 39 124110.0 40 124097.0 41 127651.0 42 132250.0 43 137954.0 44 144932.0 45 149398.5 46 152398.5 47 161332.0 48 196923.0 49 201954.0 50 173120.0 51 162042.5 52 155039.5 53 154033.0 54 151350.0 55 140476.0 56 128569.0 57 116286.5 58 106731.5 59 97441.5 60 84880.5 61 70087.5 62 55475.0 63 42726.5 64 31383.5 65 23680.5 66 18265.5 67 14741.5 68 11363.0 69 8206.5 70 6222.5 71 4742.0 72 3682.0 73 3094.0 74 2477.5 75 1908.5 76 1388.5 77 907.5 78 641.5 79 459.0 80 303.5 81 224.0 82 189.0 83 155.5 84 123.0 85 84.5 86 55.0 87 40.5 88 35.0 89 26.0 90 20.0 91 14.5 92 10.0 93 9.5 94 10.0 95 7.0 96 4.5 97 7.0 98 7.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2709262836771534 2 0.1669201559387983 3 0.193471635672342 4 0.16458837727122944 5 0.23630157025495138 6 0.1954734456605379 7 0.2412950962694621 8 0.24745451161775728 9 0.15053171152994158 10-11 0.20586745906078593 12-13 0.212125865012893 14-15 0.1740254815012959 16-17 0.2870727510544699 18-19 0.23225395445464317 20-21 0.19877313245426745 22-23 0.24551869536543594 24-25 0.23580661723589194 26-27 0.3447732741208204 28-29 0.31601100423547795 30-31 0.2905154242759277 32-33 0.19245973172226494 34-35 0.3594898772208542 36-37 0.39677633798999795 38-39 0.3782210992532589 40-41 0.3406816624965958 42-43 0.37356854087410024 44-45 0.3822467171416089 46-47 0.38759220974745073 48-49 0.4064334213396465 50-51 0.3993610926450861 52-53 0.3990861187456086 54-55 0.39349864910822663 56-57 0.3726116317039187 58-59 0.3467420872410791 60-61 0.3142401723228431 62-63 0.3812678100594692 64-65 0.4148586216196359 66-67 0.42071006620051626 68-69 0.42508765068019744 70-71 0.4242737279377441 72-73 0.4159805151295039 74-75 0.41162492856178096 76-77 0.39564344552415087 78-79 0.3704888331999527 80-81 0.38124581214751097 82-83 0.39053992994984915 84-85 0.3911778693966369 86-87 0.3867782870049975 88-89 0.3949285133855095 90-91 0.4163214827648559 92-93 0.4151225965631342 94-95 0.42395475821435036 96-97 0.4245157049692843 98-99 0.4220959346538826 100-101 0.394763529045823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4545886.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.56384875063453 #Duplication Level Percentage of deduplicated Percentage of total 1 66.21567367366676 23.54884203451657 2 14.13387135204593 10.053097260501756 3 6.5040747593566515 6.939297930137389 4 3.663711933200849 5.2118278823299935 5 2.227904917152287 3.9616436752199453 6 1.499749016345528 3.2002108308735155 7 1.0450180111446066 2.6015403743024987 8 0.7665290104903162 2.1808577433640925 9 0.6056541954122471 1.9385454780745566 >10 2.957714778886699 18.709055899583824 >50 0.22166092312954666 5.5359413690294055 >100 0.14474491118677993 9.672050795552776 >500 0.009460285334245052 2.2878202334903137 >1k 0.003858800355182544 2.495153821333945 >5k 1.8671614621850816E-4 0.49685273117782536 >10k+ 1.8671614621850816E-4 1.1672619405115436 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23909 0.5259480770085303 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16715 0.36769509838126163 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12111 0.26641671172572295 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8673 0.19078789041344194 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7639 0.16804204944866633 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6135 0.13495718986353816 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4764 0.10479805256885016 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4724 0.10391813609052229 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.399582391639386E-5 0.0 2 0.0 0.0 0.0 6.599373587459079E-5 0.0 3 0.0 0.0 0.0 1.3198747174918157E-4 0.0 4 0.0 0.0 0.0 2.1997911958196927E-4 0.0 5 0.0 0.0 0.0 2.419770315401662E-4 0.0 6 0.0 0.0 0.0 3.959624152475447E-4 0.0 7 0.0 0.0 0.0 4.1796032720574166E-4 0.0 8 0.0 0.0 0.0 4.1796032720574166E-4 0.0 9 0.0 0.0 0.0 5.719457109131202E-4 0.0 10-11 0.0 0.0 0.0 9.899060381188618E-4 0.0 12-13 0.0 0.0 0.0 0.001253880981617225 0.0 14-15 0.0 0.0 0.0 0.0012758788935754218 0.0 16-17 0.0 0.0 0.0 0.0015288548810946864 0.0 18-19 0.0 1.0998955979098465E-5 0.0 0.001847824604488542 0.0 20-21 0.0 2.199791195819693E-5 0.0 0.002265784931694283 0.0 22-23 0.0 2.199791195819693E-5 0.0 0.0029367212464192897 0.0 24-25 0.0 2.199791195819693E-5 0.0 0.0038056387687680684 0.0 26-27 0.0 2.199791195819693E-5 0.0 0.0052904978259463616 0.0 28-29 0.0 2.199791195819693E-5 0.0 0.009283118846359104 0.0 30-31 0.0 2.199791195819693E-5 0.0 0.021997911958196927 0.0 32-33 0.0 2.199791195819693E-5 0.0 0.045128716382241 0.0 34-35 0.0 2.199791195819693E-5 0.0 0.078752524810345 0.0 36-37 0.0 2.199791195819693E-5 0.0 0.13275739866771846 0.0 38-39 0.0 2.199791195819693E-5 0.0 0.2126428159439106 0.0 40-41 0.0 2.199791195819693E-5 0.0 0.3044730994134037 0.0 42-43 0.0 2.199791195819693E-5 0.0 0.39954807489673083 0.0 44-45 0.0 2.199791195819693E-5 0.0 0.5122983726384691 1.0998955979098465E-5 46-47 0.0 2.199791195819693E-5 0.0 0.6506102440756323 2.199791195819693E-5 48-49 0.0 2.199791195819693E-5 0.0 0.8024728292790448 2.199791195819693E-5 50-51 0.0 2.199791195819693E-5 0.0 0.9674351710535636 2.199791195819693E-5 52-53 0.0 2.199791195819693E-5 0.0 1.1277449544489238 2.199791195819693E-5 54-55 0.0 2.199791195819693E-5 0.0 1.3039922250579976 2.199791195819693E-5 56-57 0.0 2.199791195819693E-5 0.0 1.5092877384078704 2.199791195819693E-5 58-59 0.0 2.199791195819693E-5 0.0 1.7443464266371835 2.199791195819693E-5 60-61 0.0 2.199791195819693E-5 0.0 2.0096082479851014 2.199791195819693E-5 62-63 0.0 2.199791195819693E-5 0.0 2.2731322342883216 2.199791195819693E-5 64-65 0.0 2.199791195819693E-5 0.0 2.539526948102086 2.199791195819693E-5 66-67 0.0 2.199791195819693E-5 0.0 2.8440000475154896 2.199791195819693E-5 68-69 0.0 2.199791195819693E-5 0.0 3.1857706066540166 2.199791195819693E-5 70-71 0.0 2.199791195819693E-5 0.0 3.569337198513117 2.199791195819693E-5 72-73 0.0 2.199791195819693E-5 0.0 3.96019609818636 2.199791195819693E-5 74-75 0.0 2.199791195819693E-5 0.0 4.358270312981892 2.199791195819693E-5 76-77 0.0 2.199791195819693E-5 0.0 4.776208642275675 2.199791195819693E-5 78-79 0.0 2.199791195819693E-5 0.0 5.244566185777646 2.199791195819693E-5 80-81 0.0 2.199791195819693E-5 0.0 5.764321850569944 2.199791195819693E-5 82-83 0.0 4.399582391639386E-5 0.0 6.291017856585053 2.199791195819693E-5 84-85 0.0 4.399582391639386E-5 0.0 6.823136347897858 2.199791195819693E-5 86-87 0.0 4.399582391639386E-5 0.0 7.382576245862743 2.199791195819693E-5 88-89 0.0 4.399582391639386E-5 0.0 7.96530093363538 2.199791195819693E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 26200 0.0 27.209068 1 TACATGG 26875 0.0 26.424746 2 ACATGGG 26960 0.0 25.0044 3 GTATCAA 39560 0.0 23.79863 1 GAGTACT 17990 0.0 22.808992 12-13 AGTACTT 18745 0.0 21.687733 12-13 CATGGGA 16420 0.0 21.668575 4 GTACTTT 19085 0.0 21.55102 14-15 CATGGGG 9815 0.0 20.735249 4 TATCAAC 46005 0.0 20.334774 2 ATCAACG 45965 0.0 20.30109 3 TCAACGC 46190 0.0 20.21247 4 CAACGCA 46440 0.0 20.052586 5 AACGCAG 46985 0.0 19.869593 6 GGTATCA 29615 0.0 19.43373 1 GTATAGG 1365 0.0 18.801168 1 ACTTTTT 22665 0.0 18.597553 16-17 TACTTTT 21855 0.0 18.363447 14-15 ATGGGAT 7675 0.0 18.357786 5 ACGCAGA 50915 0.0 18.271698 7 >>END_MODULE