FastQCFastQC Report
Thu 26 May 2016
SRR938720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4545886
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225990.4971308123432923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175080.3851394425641118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130710.28753470720559204No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94400.207660288885379No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA75480.16604023946047042No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA64700.14232649036953413No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50910.11199136977918056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG259250.023.594641
TACATGG267750.022.6163332
GTATCAA416150.022.1625181
GAGTACT195650.021.77982912-13
ACATGGG269550.021.4265483
AGTACTT204650.021.36690312-13
GTACTTT208850.020.3010314-15
CATGGGA159600.019.6845764
GGTATCA319850.019.2730221
ATCAACG486400.018.811113
TATCAAC489850.018.7270662
CAACGCA491000.018.6445435
TCAACGC490700.018.6365974
GGACCGT3851.2732926E-1118.4854986
AACGCAG500300.018.3127026
TACTTTT235700.018.3004714-15
CATGGGG99700.017.8023554
ACTTTTT238400.017.75346616-17
AGTACAT203750.017.2825282
GTATAGG14600.016.9411011
Produced by FastQC (version 0.11.5)