##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938719_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1321575 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88442275315438 31.0 31.0 34.0 30.0 34.0 2 32.0572211187409 33.0 31.0 34.0 30.0 34.0 3 32.1272481697974 34.0 31.0 34.0 30.0 34.0 4 35.76206647371507 37.0 35.0 37.0 35.0 37.0 5 35.69671414789172 37.0 35.0 37.0 33.0 37.0 6 35.784314170591905 37.0 35.0 37.0 33.0 37.0 7 35.73017952064771 37.0 35.0 37.0 33.0 37.0 8 35.73434349166714 37.0 35.0 37.0 33.0 37.0 9 37.30803775797817 39.0 37.0 39.0 34.0 39.0 10-11 37.22513516069841 39.0 37.0 39.0 33.0 39.0 12-13 37.149692979967085 39.0 37.0 39.0 33.0 39.0 14-15 38.123423188241304 40.0 37.0 41.0 33.0 41.0 16-17 38.08895711556287 40.0 37.0 41.0 33.0 41.0 18-19 38.25828689253353 40.0 37.5 41.0 33.5 41.0 20-21 38.3710648279515 40.0 38.0 41.0 34.0 41.0 22-23 38.316019143824604 40.0 37.5 41.0 33.5 41.0 24-25 38.19973554281823 40.0 37.0 41.0 33.0 41.0 26-27 37.8775245445775 40.0 36.5 41.0 33.0 41.0 28-29 37.65517507519437 40.0 36.5 41.0 33.0 41.0 30-31 37.39050943003613 39.0 36.0 41.0 32.0 41.0 32-33 37.0834557251764 39.0 35.0 41.0 32.0 41.0 34-35 36.96571515048333 39.0 35.0 41.0 31.0 41.0 36-37 36.7467389289295 39.0 35.0 41.0 31.0 41.0 38-39 36.529456898019404 39.0 35.0 41.0 30.5 41.0 40-41 36.30774870892685 39.0 35.0 41.0 30.0 41.0 42-43 36.14595425912263 39.0 35.0 41.0 30.0 41.0 44-45 35.847732818795755 39.0 35.0 41.0 29.5 41.0 46-47 35.3878550214706 38.0 35.0 41.0 26.5 41.0 48-49 35.01485916425477 38.0 35.0 40.0 25.0 41.0 50-51 34.77387019276242 38.0 34.0 40.0 24.0 41.0 52-53 34.46662769801184 38.0 34.0 40.0 24.0 41.0 54-55 34.28404706505495 38.0 34.0 40.0 23.0 41.0 56-57 34.10779183928267 38.0 34.0 40.0 23.0 41.0 58-59 33.87091879008002 37.5 33.0 40.0 22.5 41.0 60-61 33.45467907610238 37.0 33.0 40.0 22.0 41.0 62-63 33.00813461211055 36.0 32.5 39.0 20.5 41.0 64-65 32.52376558273272 36.0 32.0 39.0 19.0 40.5 66-67 32.10322342659327 35.0 31.0 39.0 18.0 40.0 68-69 31.72125948205739 35.0 31.0 38.0 14.0 40.0 70-71 31.183293040500917 35.0 31.0 37.0 10.5 39.0 72-73 30.772743128464143 35.0 30.5 36.5 7.5 39.0 74-75 30.363943022529938 34.5 30.0 36.0 4.5 38.5 76-77 28.2530435276091 32.0 27.0 34.5 2.0 36.0 78-79 29.29763501882224 34.0 29.0 35.0 2.0 37.0 80-81 29.408305998524487 34.0 29.0 35.0 2.0 36.5 82-83 29.131963377031195 34.0 29.0 35.0 2.0 36.0 84-85 28.7764459073454 34.0 29.0 35.0 2.0 36.0 86-87 28.21689537105348 34.0 29.0 35.0 2.0 35.0 88-89 27.593682537880937 33.0 26.5 35.0 2.0 35.0 90-91 27.069236706202826 33.0 25.5 35.0 2.0 35.0 92-93 26.850911223350927 33.0 25.0 35.0 2.0 35.0 94-95 26.51525793087793 33.0 25.0 35.0 2.0 35.0 96-97 25.87807578079186 33.0 24.0 35.0 2.0 35.0 98-99 25.114084331195734 32.0 19.0 35.0 2.0 35.0 100-101 21.989937763653217 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15410675582988986 1101 2 0.18202252345150782 1101 3 0.2067510958163581 1101 4 0.1693535741952914 1101 5 0.18088712952428665 1101 6 0.24237992251460128 1101 7 0.23214942717503106 1101 8 0.19364062906033297 1101 9 0.25813094701281614 1101 10-11 0.224844090073411 1101 12-13 0.25575118867621427 1101 14-15 0.29184563563699584 1101 16-17 0.37186259101922303 1101 18-19 0.24858158175540268 1101 20-21 0.38569044272354347 1101 22-23 0.3671242912989783 1101 24-25 0.38504450412763447 1101 26-27 0.4366914445133503 1101 28-29 0.4574550292125892 1101 30-31 0.40353275348034146 1101 32-33 0.40026116238534826 1101 34-35 0.28827696974789063 1101 36-37 0.40074708278415727 1101 38-39 0.487490021526213 1101 40-41 0.5633117496353393 1101 42-43 0.4236139259318463 1101 44-45 0.5172040467825951 1101 46-47 0.6821912264465269 1101 48-49 0.4729508152748636 1101 50-51 0.2893828644197214 1101 52-53 0.25140693618534726 1101 54-55 0.42322367076486955 1101 56-57 0.2502669526294028 1101 58-59 0.2664806872329919 1101 60-61 0.2954864007834459 1101 62-63 0.4261165517855474 1101 64-65 0.410603029943573 1101 66-67 0.3446678630273894 1101 68-69 0.37151726918497374 1101 70-71 0.3076472597177329 1101 72-73 0.4127585514337788 1101 74-75 0.46808136496574804 1101 76-77 0.46614434246453484 1101 78-79 0.580418771970816 1101 80-81 0.6592087249448397 1101 82-83 0.776564599618851 1101 84-85 0.8229518894985901 1101 86-87 0.9462106670528421 1101 88-89 0.8981825848423881 1101 90-91 0.9148825043130024 1101 92-93 1.0552870788343007 1101 94-95 1.049866845625015 1101 96-97 1.1148222237312098 1101 98-99 1.2902321971351363 1101 100-101 1.706290707323376 1102 1 0.09163323971819892 1102 2 0.08912178664601811 1102 3 0.16085412209470462 1102 4 0.1321022065198676 1102 5 0.17626391224573013 1102 6 0.20541948232866503 1102 7 0.09969520082079697 1102 8 0.16991738964598113 1102 9 0.23384482615141877 1102 10-11 0.20762086647033584 1102 12-13 0.18902285803079621 1102 14-15 0.36502022375323406 1102 16-17 0.3945565163267517 1102 18-19 0.3664198640183116 1102 20-21 0.2855781826596626 1102 22-23 0.3068437992520501 1102 24-25 0.15033242747456654 1102 26-27 0.10422615026549664 1102 28-29 0.017360794004041225 1102 30-31 0.12193707944562249 1102 32-33 0.17073044107491597 1102 34-35 0.3906834460467863 1102 36-37 0.41674493245054123 1102 38-39 0.4835008364394042 1102 40-41 0.66853895768012 1102 42-43 0.5790972766575848 1102 44-45 0.6214082652311959 1102 46-47 0.78152715282922 1102 48-49 0.6130940654408832 1102 50-51 0.6445499896134095 1102 52-53 0.6688752345757578 1102 54-55 0.7724805407645476 1102 56-57 0.7586358613350868 1102 58-59 0.6322461111333197 1102 60-61 0.6516701910626708 1102 62-63 0.6973163114541379 1102 64-65 0.6303592200583594 1102 66-67 0.7364839462579482 1102 68-69 0.749273965260624 1102 70-71 0.6827097142603122 1102 72-73 0.8394464052110955 1102 74-75 0.8515475130225383 1102 76-77 0.6328695108546327 1102 78-79 0.7685919370721628 1102 80-81 0.9363487812329829 1102 82-83 0.9480378943726713 1102 84-85 0.9374745470579597 1102 86-87 0.7022142087788019 1102 88-89 0.7567728514205072 1102 90-91 0.9782288028931525 1102 92-93 1.1347529739106683 1102 94-95 1.1887262201309134 1102 96-97 1.3685189002008684 1102 98-99 1.7716824074251143 1102 100-101 1.9990728595543459 1103 1 -0.021077196410089982 1103 2 -0.04967991000016525 1103 3 -0.028234839054107397 1103 4 0.04662457054696034 1103 5 -0.05989069056489171 1103 6 -0.0793614510773466 1103 7 0.14200484501379407 1103 8 0.0701905571991901 1103 9 0.08879661458440324 1103 10-11 -0.005099280889780289 1103 12-13 0.047870416752424205 1103 14-15 0.020138042923839805 1103 16-17 -0.007920474319135451 1103 18-19 0.09621016406404692 1103 20-21 0.036397998584831726 1103 22-23 -0.05087600351596677 1103 24-25 -0.024156793058111248 1103 26-27 0.0058187922844368245 1103 28-29 0.06531798940029176 1103 30-31 0.09089747641571222 1103 32-33 0.11954942997905249 1103 34-35 -0.061659153681297596 1103 36-37 -0.13972118161570535 1103 38-39 0.036635058193816405 1103 40-41 0.02192034594769865 1103 42-43 0.04920140612397006 1103 44-45 -0.19509894927425364 1103 46-47 0.0017675528066689594 1103 48-49 0.07845034234255621 1103 50-51 0.11714583320619454 1103 52-53 -0.0783583174169209 1103 54-55 -0.08540028049240078 1103 56-57 0.03964194495193141 1103 58-59 -0.29542369445407957 1103 60-61 -0.14393281378040967 1103 62-63 -0.13362523946090477 1103 64-65 -0.3224752065717169 1103 66-67 -0.49048759043484225 1103 68-69 -0.36490036074856746 1103 70-71 -0.3968904336010226 1103 72-73 -0.32164758071701627 1103 74-75 -0.21291658361361243 1103 76-77 -0.2767690433622363 1103 78-79 -0.5119621043665994 1103 80-81 -0.47707746666030815 1103 82-83 -0.5458007989340992 1103 84-85 -0.6263294990797874 1103 86-87 -0.49900596050989776 1103 88-89 -0.32174997623958745 1103 90-91 -0.34948235374588776 1103 92-93 -0.44646707007181163 1103 94-95 -0.5393705663911526 1103 96-97 -0.24393685104123364 1103 98-99 -0.0367839071310776 1103 100-101 0.48992049345970656 1104 1 -0.04707099718300256 1104 2 -0.10555017265289734 1104 3 -0.08184102042432784 1104 4 -0.028428547587047603 1104 5 -0.023648498417806252 1104 6 -0.0019398855372330104 1104 7 -0.12064788847374075 1104 8 0.03928068971400478 1104 9 -0.1514457561313094 1104 10-11 -0.05055615716946704 1104 12-13 -0.040141595607416036 1104 14-15 -0.023478550927855224 1104 16-17 0.12493790266094607 1104 18-19 -0.03150501414864948 1104 20-21 0.002232270006729209 1104 22-23 -0.011881682722247433 1104 24-25 0.02843136615270936 1104 26-27 -0.19086791509105439 1104 28-29 -0.08299049514327095 1104 30-31 -0.2254543423902149 1104 32-33 -0.13860620947158964 1104 34-35 -0.08873492348703138 1104 36-37 -0.04751582286771594 1104 38-39 0.010729938462347377 1104 40-41 -0.15613700836503597 1104 42-43 -0.10531927586340117 1104 44-45 -0.13473370403799834 1104 46-47 -0.06841926858729863 1104 48-49 -0.05800052172448744 1104 50-51 0.06776768638658837 1104 52-53 -0.22754757965098094 1104 54-55 -0.355507047640117 1104 56-57 -0.35504298397655276 1104 58-59 -0.29118962717955554 1104 60-61 -0.23730007515300144 1104 62-63 -0.30722622062312155 1104 64-65 -0.04309282477992582 1104 66-67 -0.09065011201690965 1104 68-69 -0.1513307738815648 1104 70-71 -0.31951170040065335 1104 72-73 -0.39413106010259824 1104 74-75 -0.4659806040862655 1104 76-77 -0.5265756945609965 1104 78-79 -0.4605352445079838 1104 80-81 -0.4075632939820295 1104 82-83 -0.4930452316348415 1104 84-85 -0.42479308495500945 1104 86-87 -0.12559633735002862 1104 88-89 -0.07503419113486132 1104 90-91 -0.1785412005545126 1104 92-93 0.08462951021831344 1104 94-95 0.14017656909055276 1104 96-97 0.010132221234421479 1104 98-99 0.12972711525877045 1104 100-101 0.7402948239781679 1105 1 -0.23457118644376962 1105 2 -0.16157559961004964 1105 3 0.005122031634194002 1105 4 -0.13966300624976924 1105 5 -0.04795260928283085 1105 6 -0.03426501918561797 1105 7 -0.11690809568550975 1105 8 -0.08793343416610355 1105 9 -0.1734539067598675 1105 10-11 -0.05540335256713291 1105 12-13 -0.04606439780538807 1105 14-15 -0.0798134570938771 1105 16-17 -0.1954287968916688 1105 18-19 -0.09769752959687139 1105 20-21 -0.11542221674531561 1105 22-23 -0.16450156654945403 1105 24-25 -0.12172086006872007 1105 26-27 -0.1766700264539125 1105 28-29 -0.18506897204527917 1105 30-31 -0.2927990773978877 1105 32-33 -0.2754832311551212 1105 34-35 -0.20100955933417453 1105 36-37 -0.17763729497128367 1105 38-39 -0.16269742567719447 1105 40-41 -0.26884346158188777 1105 42-43 -0.3830278094346298 1105 44-45 -0.28563510008598314 1105 46-47 -0.42859068736475336 1105 48-49 -0.5390726279576157 1105 50-51 -0.6992398919174079 1105 52-53 -0.5744422798043942 1105 54-55 -0.572853811523224 1105 56-57 -0.5575931895591637 1105 58-59 -0.48104149504707294 1105 60-61 -0.6884178038299709 1105 62-63 -0.740101330924297 1105 64-65 -0.6656593098168173 1105 66-67 -0.7002084773126072 1105 68-69 -0.6209854592817159 1105 70-71 -0.47005577211746186 1105 72-73 -0.6502511477257933 1105 74-75 -0.7068256821858583 1105 76-77 -0.7548426533857295 1105 78-79 -0.6320927122534066 1105 80-81 -0.6628046060042969 1105 82-83 -0.6843403264196617 1105 84-85 -0.5610438806072793 1105 86-87 -0.6339168463736797 1105 88-89 -0.29113970719296134 1105 90-91 -0.22117271861968035 1105 92-93 -0.24938152807534664 1105 94-95 -0.12713110108818526 1105 96-97 -0.08365754245306789 1105 98-99 0.229501153041884 1105 100-101 0.7652696750072323 1106 1 -0.10866198506809255 1106 2 -0.09659915354106374 1106 3 -0.1319056316538365 1106 4 -0.00750323719857704 1106 5 -0.09524028636690218 1106 6 -0.2115669587333926 1106 7 -0.09291536684170154 1106 8 -0.11171320797963347 1106 9 -0.18586850995161797 1106 10-11 -0.0489754246502585 1106 12-13 -0.07144440286230491 1106 14-15 -0.009223774182082423 1106 16-17 -0.10262090810451951 1106 18-19 -0.12184951443594372 1106 20-21 -0.06348389637749108 1106 22-23 -0.18004513579850112 1106 24-25 -0.30504016005423296 1106 26-27 -0.26702279536026907 1106 28-29 -0.08828152448622006 1106 30-31 -0.32130988962187246 1106 32-33 -0.2670086028751797 1106 34-35 -0.36576617689639335 1106 36-37 -0.33549128514712123 1106 38-39 -0.3939739530004829 1106 40-41 -0.35109412008548446 1106 42-43 -0.42160427563307934 1106 44-45 -0.32906258368170427 1106 46-47 -0.3377159638380718 1106 48-49 -0.3102582781025731 1106 50-51 -0.20897445298456319 1106 52-53 -0.2449720039079537 1106 54-55 -0.38146124518803504 1106 56-57 -0.4129467071835009 1106 58-59 -0.2977283320941311 1106 60-61 -0.34649456828820746 1106 62-63 -0.253973172500487 1106 64-65 -0.12968157006135783 1106 66-67 -0.2394501978419612 1106 68-69 -0.22471603864213918 1106 70-71 -0.33624667556230037 1106 72-73 -0.24966647263256903 1106 74-75 0.07974305380636437 1106 76-77 -0.10278785213966302 1106 78-79 -0.07237600995103932 1106 80-81 -0.14495228510131852 1106 82-83 -0.11735093366675997 1106 84-85 -0.21681250711484168 1106 86-87 -0.11854940995947416 1106 88-89 -0.0032490543845291597 1106 90-91 0.11181990712197987 1106 92-93 0.08497014352958132 1106 94-95 0.11847877266077944 1106 96-97 -0.006623361636449943 1106 98-99 0.10988946764090102 1106 100-101 0.4618210309232076 1107 1 -0.10192626306653807 1107 2 -0.051566831217613185 1107 3 -0.10690424830169576 1107 4 -0.04644159262144143 1107 5 -0.054233518767659916 1107 6 -0.10243912015494772 1107 7 -0.19671068365340005 1107 8 -0.13327666053787368 1107 9 -0.1198338948723574 1107 10-11 -0.020294324608642 1107 12-13 -0.05987326367783652 1107 14-15 -0.1580048003647292 1107 16-17 -0.20923886473703135 1107 18-19 -0.06794599542868696 1107 20-21 -0.2601695049441659 1107 22-23 -0.23535847107253005 1107 24-25 -0.21358494577900444 1107 26-27 -0.2380334483746509 1107 28-29 -0.14671161227494878 1107 30-31 -0.22075402264501776 1107 32-33 -0.12671444206470284 1107 34-35 -0.1457789037360726 1107 36-37 -0.08288366636529076 1107 38-39 -0.1651302188639221 1107 40-41 -0.15315056325858478 1107 42-43 -0.07043161403951359 1107 44-45 0.13013634716753586 1107 46-47 0.04196969043937315 1107 48-49 -0.2924455142131137 1107 50-51 -0.264893155183735 1107 52-53 -0.13469237430951608 1107 54-55 -0.08025195483565994 1107 56-57 -0.1337955681433769 1107 58-59 -0.30268193566630686 1107 60-61 -0.2111693482149235 1107 62-63 -0.40033754264221244 1107 64-65 -0.4170771871206185 1107 66-67 -0.16652979712694815 1107 68-69 -0.035133041122549 1107 70-71 -0.19050902408524095 1107 72-73 -0.3945314093472163 1107 74-75 -0.2986939748013562 1107 76-77 -0.4463776389109704 1107 78-79 -0.294150513929079 1107 80-81 -0.3721435148893413 1107 82-83 -0.04231430490795063 1107 84-85 -0.09574844467874755 1107 86-87 -0.20017169708563998 1107 88-89 -0.14344998928999075 1107 90-91 -0.03112156866214022 1107 92-93 0.09831528146783342 1107 94-95 0.27052571280614046 1107 96-97 0.2957085490495217 1107 98-99 0.23045071164737507 1107 100-101 0.7067218746477195 1108 1 -0.10507799546644137 1108 2 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-1.3799097298732335 2316 74-75 -1.4279686712579007 2316 76-77 -1.295237814349477 2316 78-79 -1.367542392050911 2316 80-81 -1.2885715852564807 2316 82-83 -1.6597833165961227 2316 84-85 -1.76703266918107 2316 86-87 -2.1188570473673387 2316 88-89 -2.1792365994116984 2316 90-91 -2.6483058110495215 2316 92-93 -2.9358418297499114 2316 94-95 -2.9239196622569317 2316 96-97 -2.701300471308002 2316 98-99 -3.1506894170116517 2316 100-101 -3.7008152085002735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 29.0 5 105.0 6 304.0 7 543.0 8 940.0 9 1507.0 10 2491.0 11 3519.0 12 4811.0 13 6698.0 14 7572.0 15 10325.0 16 12993.0 17 17751.0 18 15923.0 19 10477.0 20 9298.0 21 9850.0 22 10501.0 23 11870.0 24 13387.0 25 15391.0 26 18053.0 27 21927.0 28 26235.0 29 31613.0 30 37650.0 31 45067.0 32 55126.0 33 68979.0 34 93486.0 35 145095.0 36 179935.0 37 243783.0 38 174546.0 39 13789.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.09147897182242 19.169294128638413 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30.823862615283975 32.89699740498731 18.042204992760524 20-21 20.58298643020125 28.160046974271975 30.488519565109645 20.76844703041713 22-23 19.384673590223787 27.728127423717915 29.899022000264836 22.988176985793466 24-25 20.83328604127651 28.138925146132454 31.225772279287973 19.802016533303064 26-27 22.097135688202098 25.576925696177437 33.436984989111465 18.888953626508997 28-29 19.093884191211245 27.246505117000545 34.64211263076254 19.01749806102567 30-31 18.815277226037114 29.881883358871043 32.57249872311447 18.730340691977375 32-33 18.69311238484384 29.633316308192875 31.518831697028165 20.154739609935117 34-35 20.124768395638707 27.018623317569546 31.715599492541934 21.141008794249817 36-37 21.67186774540332 26.79685067019939 32.956562573196344 18.574719011200955 38-39 22.39745760082456 25.81122406122463 33.56907559921872 18.222242738732085 40-41 19.137280185488507 27.74298733204009 34.644889625939335 18.474842856532074 42-43 18.344529341992505 29.975728844083832 31.906903185896624 19.77283862802704 44-45 19.092112222683802 29.72363533454252 30.112383101371044 21.071869341402643 46-47 19.492401381401923 29.05052831662071 29.012656293036393 22.444414008940974 48-49 20.660644550092957 28.408986866408437 29.752575150975392 21.177793432523213 50-51 21.35679276013991 29.338572042018125 30.39496508893761 18.909670108904354 52-53 19.40903845790061 29.931861604524908 31.045759794184967 19.613340143389514 54-55 17.53570550290373 28.851446191097747 32.06227418042866 21.55057412556987 56-57 20.133439267540624 30.633675727824755 28.510451544558574 20.722433460076044 58-59 20.134884818988297 28.548955335010124 29.55726210906405 21.758897736937527 60-61 19.671677761446162 27.79506154623203 31.244412411776167 21.28884828054564 62-63 22.378033785445396 27.351682651381875 30.251782910542346 20.018500652630383 64-65 20.920265592191136 28.641431625144243 31.217713712804795 19.220589069859827 66-67 19.18714369813902 28.735844402305755 31.142390595371232 20.934621304183988 68-69 18.542062425207074 30.783604747551298 30.268117622622565 20.40621520461907 70-71 19.51248856928066 30.333506488789475 28.2899946539987 21.864010287931166 72-73 19.376993360195222 28.568374855759227 28.449728543593817 23.604903240451733 74-75 19.564156404290337 30.221742996046387 28.81641223540094 21.397688364262336 76-77 20.946680577672836 27.525046438397403 30.26045266313085 21.26782032079891 78-79 19.440612426486755 27.51975523529347 31.847191613387427 21.19244072483235 80-81 19.170308155042278 30.60609500028375 29.856156479957622 20.36744036471634 82-83 18.903126950797343 29.717079999243328 29.06452528233358 22.31526776762575 84-85 19.165087111968674 28.103475020335583 29.05340219056807 23.67803567712767 86-87 20.43407209426657 28.249430991281955 29.6671502656637 21.649346648787777 88-89 21.323869620913793 26.45218959054894 30.15119798832851 22.072742800208758 90-91 19.42137332122882 27.046914353979673 31.509739822476384 22.02197250231512 92-93 18.743733802470537 29.733121464918753 30.17649395607514 21.346650776535572 94-95 18.499221950786733 29.789240031492735 28.955726672994974 22.75581134472555 96-97 18.17948281406655 28.300323477668694 28.465429506460094 25.054764201804662 98-99 19.78685192114376 28.067309362088032 28.138058573536995 24.007780143231212 100-101 20.69817171446602 27.594800411027524 29.296720708613872 22.410307165892583 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11892.0 1 7392.0 2 4525.5 3 5620.0 4 4061.0 5 3632.0 6 6272.5 7 11949.0 8 16022.5 9 15351.5 10 11030.5 11 6941.0 12 5917.0 13 5600.0 14 5237.0 15 4751.5 16 4251.5 17 4013.5 18 3878.0 19 3843.0 20 4015.5 21 4271.0 22 4517.5 23 4898.0 24 5258.5 25 5798.5 26 6894.0 27 7969.5 28 8936.0 29 10090.5 30 11447.0 31 13089.0 32 15794.0 33 18900.5 34 21741.0 35 23733.0 36 27434.5 37 31386.0 38 31557.5 39 30426.5 40 29035.0 41 28425.5 42 27832.5 43 28226.0 44 31580.0 45 34183.5 46 37863.0 47 56320.5 48 137031.0 49 136035.5 50 61923.0 51 45729.5 52 33337.5 53 27612.5 54 24340.5 55 21709.0 56 19597.0 57 17825.5 58 16291.0 59 15045.0 60 13870.5 61 12326.5 62 10310.5 63 7687.0 64 5309.0 65 4074.5 66 3126.0 67 2431.0 68 1824.5 69 1229.0 70 960.5 71 819.5 72 725.0 73 697.0 74 583.0 75 439.0 76 308.5 77 188.0 78 129.5 79 89.5 80 51.5 81 29.0 82 16.0 83 7.5 84 4.5 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.380757807918582 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.648355182263587E-4 16-17 0.0020051832094281448 18-19 1.1350093638272516E-4 20-21 3.7833645460908383E-5 22-23 0.0 24-25 0.0 26-27 7.566729092181677E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006545220664737151 36-37 0.016230633902729698 38-39 0.01104742447458525 40-41 0.006923557119346235 42-43 0.0035941963187862964 44-45 0.0018160149821236026 46-47 0.0014376785275145186 48-49 1.5133458184363353E-4 50-51 1.8916822730454194E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.2700187276545033E-4 60-61 5.675046819136258E-4 62-63 0.0 64-65 0.0 66-67 9.83674781983618E-4 68-69 7.188392637572593E-4 70-71 0.0020051832094281448 72-73 0.0 74-75 0.0 76-77 0.09666496415262094 78-79 0.035525793087792974 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.1479673873976127 88-89 0.322531827554244 90-91 0.2731210865822976 92-93 0.0 94-95 3.7833645460908383E-5 96-97 0.0 98-99 4.1617010006999227E-4 100-101 6.810056182963509E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1321575.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.319831163936538 #Duplication Level Percentage of deduplicated Percentage of total 1 69.85639177226396 19.783212207123206 2 13.40265812384564 7.59122030430542 3 5.524853406602465 4.693887470414443 4 3.0228266761726146 3.424237644282077 5 1.8615886628773912 2.635993831469305 6 1.1936104246780281 2.028170742143781 7 0.847230400377145 1.6795395326944569 8 0.6309026206227433 1.4293644557536958 9 0.46082602676372 1.1745463746506626 >10 2.7562427640202203 14.735661248457358 >50 0.26374731180931893 5.217142659321685 >100 0.14868978861222834 7.689410963441855 >500 0.010944849785371717 2.0538762695249293 >1k 0.01494906312148332 9.941810459968158 >5k 0.001868632890185415 3.5638170355445635 >10k+ 0.0026694755574077356 12.358108800904398 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 28203 2.1340446058679983 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 25135 1.9018973573198645 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18834 1.425117757221497 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 15740 1.191003159109396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15446 1.168756975578382 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14038 1.0622174299604639 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12973 0.981631765128729 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12526 0.9478084860866768 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 10537 0.7973062444431833 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10037 0.7594725989822749 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 9047 0.6845619809696764 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7914 0.598830940355258 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7282 0.5510092124926697 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 6950 0.5258876719066266 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5804 0.4391729565102246 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5092 0.385297845373891 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5052 0.38227115373701837 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4968 0.3759151012995857 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4828 0.3653216805705314 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4770 0.360932977697066 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4733 0.3581332879329588 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4488 0.33959480165711364 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4384 0.33172540340124473 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4345 0.3287743790552939 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4262 0.3224939939087831 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4128 0.31235457692525964 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3634 0.27497493520988214 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3599 0.27232658002761856 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3453 0.2612791555530333 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3382 0.2559067778975843 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3366 0.25469610124283526 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3139 0.23751962620358283 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3066 0.2319959139662902 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3027 0.22904488962033936 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2874 0.21746779410930142 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2692 0.20369634716153073 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2562 0.19385959934169458 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2509 0.18984923292283828 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2429 0.18379584964909293 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2328 0.17615345326598944 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2246 0.16994873541040048 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2183 0.16518169608232602 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2079 0.15731229782645706 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2043 0.15458827535327166 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2021 0.1529235949529917 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1929 0.14596220418818454 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1866 0.1411951648601101 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1865 0.14111949756918826 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1779 0.13461211054991204 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1710 0.12939106747630666 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1671 0.12644004313035584 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1641 0.12417002440270133 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1547 0.11705729905605056 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1421 0.10752322039990164 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1395 0.10555587083593439 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1369 0.10358852127196716 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1351 0.10222651003537445 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1347 0.1019238408716872 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.1350093638272515E-4 0.0 16-17 0.0 0.0 0.0 4.161701000699922E-4 0.0 18-19 0.0 0.0 0.0 6.810056182963509E-4 0.0 20-21 0.0 0.0 0.0 0.0015133458184363353 0.0 22-23 0.0 0.0 0.0 0.003253693509638121 0.0 24-25 0.0 0.0 0.0 0.006015549628284433 0.0 26-27 0.0 0.0 0.0 0.00960974594707073 0.0 28-29 0.0 0.0 0.0 0.018614153566766926 0.0 30-31 0.0 0.0 0.0 0.04290335395267011 0.0 32-33 0.0 0.0 0.0 0.08357452282314662 0.0 34-35 0.0 0.0 0.0 0.14887539488867452 0.0 36-37 0.0 0.0 0.0 0.24455668425931182 0.0 38-39 0.0 0.0 0.0 0.3619923197699715 0.0 40-41 0.0 0.0 0.0 0.49403174242854175 0.0 42-43 0.0 0.0 0.0 0.6186557705767739 0.0 44-45 0.0 0.0 0.0 0.7647693093468022 0.0 46-47 0.0 0.0 0.0 0.9509865123053931 0.0 48-49 0.0 0.0 0.0 1.1531695136484874 0.0 50-51 0.0 0.0 0.0 1.3659081020751755 0.0 52-53 0.0 0.0 0.0 1.5703232885004634 3.7833645460908383E-5 54-55 0.0 0.0 0.0 1.7809810264268013 7.566729092181677E-5 56-57 0.0 0.0 0.0 2.0213760096854134 7.566729092181677E-5 58-59 0.0 0.0 0.0 2.331498401528479 7.566729092181677E-5 60-61 0.0 0.0 0.0 2.755348731627036 7.566729092181677E-5 62-63 0.0 0.0 0.0 3.116546544842328 7.566729092181677E-5 64-65 0.0 0.0 0.0 3.410324801846282 7.566729092181677E-5 66-67 0.0 0.0 0.0 3.743336549193198 7.566729092181677E-5 68-69 0.0 0.0 0.0 4.12454836085731 7.566729092181677E-5 70-71 0.0 0.0 0.0 4.567088511813556 7.566729092181677E-5 72-73 0.0 0.0 0.0 5.0376633940563345 7.566729092181677E-5 74-75 0.0 0.0 0.0 5.499650038779487 7.566729092181677E-5 76-77 0.0 0.0 0.0 5.931445434424834 7.566729092181677E-5 78-79 0.0 0.0 0.0 6.4323250666818 7.566729092181677E-5 80-81 0.0 0.0 0.0 6.985188127802054 7.566729092181677E-5 82-83 0.0 0.0 0.0 7.53105196451204 7.566729092181677E-5 84-85 0.0 0.0 0.0 8.224013014774037 7.566729092181677E-5 86-87 0.0 0.0 0.0 8.970281671490456 7.566729092181677E-5 88-89 0.0 0.0 0.0 9.580273537256684 7.566729092181677E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 90 1.207809E-8 42.178417 2 TACCTGG 805 0.0 35.366997 2 ATAACGC 135 3.1286618E-10 35.14868 3 TAACGCA 155 1.371518E-9 30.613365 4 GCTTATG 335 0.0 29.86727 1 CATATAG 230 5.456968E-12 26.819973 3 CATTCCG 200 7.2759576E-10 26.097895 9 AGTACTC 675 0.0 26.010025 5 GTAAGCG 125 1.4551915E-11 24.674376 94-95 TCGAGTA 50 0.0016642365 23.725359 18-19 TGCACCG 125 2.2720637E-4 22.776346 5 GAGTACG 215 4.281901E-8 22.160654 1 CTTTGCG 280 6.730261E-11 22.03069 2 ACCTGGG 1220 0.0 21.78066 3 CATGGGG 2845 0.0 21.682226 4 GAGTACT 11570 0.0 21.65508 12-13 GTGCGAA 55 0.0028957338 21.568508 18-19 AGTACTT 11935 0.0 21.171734 12-13 CATGGGT 965 0.0 21.143845 4 ATGGGTA 450 0.0 21.089209 5 >>END_MODULE