FastQCFastQC Report
Thu 26 May 2016
SRR938718_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938718_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320639
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA287732.178718029681086No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA244021.8477418885857528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206371.5626526249792712No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT170641.2921017780029214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147971.1204424524794436No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT128410.9723323330599809No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125030.9467386621173537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118200.8950212737924595No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA99230.7513786886499642No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG95800.7254064131075941No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT89950.6811096749376627No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT77660.5880486643208326No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG71450.5410259730327516No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC71410.5407230893529571No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58030.4394084984617295No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53090.4020023640071208No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG52920.4007151083679946No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52280.39586896949128414No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52270.3957932485713356No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51520.39011417957519046No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT44720.33862395401014206No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44170.33445930341296903No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG41420.31363605042710385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40230.30462526095322034No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA39550.2994762383967155No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG37810.286300798325659No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36950.2797887992100794No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33460.25336219814801775No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT32860.2488189429511017No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA31870.24132257187619022No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.24094396727644724No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31340.2373093631189144No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29150.22072648165017086No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27050.20482508846096475No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT26180.1982373684254365No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG25490.19301262494898302No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24430.18498620743443137No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24110.18256313799607615No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24070.18226025431628173No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC20850.15787811809283234No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA20730.1569694670534491No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT20660.15643942061380892No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19220.14553560814121044No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.1401594228248598No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT18270.13834212074609337No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC18110.13713058602691575No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC17590.13319309818958852No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17090.1294070521921585No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC16990.1286498429926725No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT15890.12032054179832642No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC15260.11555012384156457No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC14850.11244556612367196No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC14220.1076751481669101No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT13850.1048734741288119No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT13720.10388910216948008No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA13650.10335905572983986No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13610.10305617205004548No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG13430.10169319549097067No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6800.039.0705382
GTACGTG905.6695717E-736.9687161
GTGCGAA753.1025993E-431.624939
GGGATAC2051.8189894E-1230.0856767
GATACGC650.00588216529.1922429
TACGTGG1153.9015667E-628.8782232
CGACCGT504.9808834E-528.47865118-19
GCTTATG3150.027.1606881
CATGGGT7400.025.6447564
CCTTTAG2800.025.4631441
AGTACTC6300.024.0979275
TTAGGCA2402.3283064E-1023.72144
GGATACG1000.001665780723.7204978
ACCTGGG11050.023.614063
CATGGGG27050.023.5021614
ATGGGAG25850.023.1249225
AGTACTT117500.022.84698312-13
GAGTACT115050.022.75584812-13
CATATAG2755.2750693E-1122.4275043
GAGTACG2354.614776E-922.248651
Produced by FastQC (version 0.11.5)