##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938718_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1320639 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.70320958263386 33.0 31.0 34.0 30.0 34.0 2 31.673854096388187 33.0 31.0 34.0 30.0 34.0 3 31.67541319012993 33.0 31.0 34.0 28.0 34.0 4 35.22664179991656 37.0 35.0 37.0 33.0 37.0 5 35.29296499649033 37.0 35.0 37.0 33.0 37.0 6 35.419375014670926 37.0 35.0 37.0 33.0 37.0 7 35.36198915827868 37.0 35.0 37.0 33.0 37.0 8 35.358701355934514 37.0 35.0 37.0 33.0 37.0 9 36.88416062224423 39.0 37.0 39.0 33.0 39.0 10-11 36.76949113270167 39.0 37.0 39.0 32.5 39.0 12-13 36.66019063498806 39.0 37.0 39.0 32.0 39.0 14-15 37.62489635699082 40.0 37.0 41.0 32.0 41.0 16-17 37.566451164928495 40.0 37.0 41.0 32.0 41.0 18-19 37.67539993896894 40.0 37.0 41.0 32.0 41.0 20-21 37.6001783984874 40.0 37.0 41.0 32.0 41.0 22-23 37.611452864863146 40.0 37.0 41.0 32.0 41.0 24-25 37.51751614180711 40.0 37.0 41.0 32.0 41.0 26-27 37.2644288105985 40.0 36.5 41.0 31.5 41.0 28-29 37.06312853096115 39.5 36.0 41.0 31.0 41.0 30-31 36.81374319552883 39.0 35.5 41.0 31.0 41.0 32-33 36.54684096108021 39.0 35.0 41.0 30.5 41.0 34-35 36.22724794588075 39.0 35.0 41.0 30.0 41.0 36-37 35.917239306123776 38.0 35.0 40.0 30.0 41.0 38-39 35.64591989180995 38.0 35.0 40.0 29.5 41.0 40-41 35.47690171197428 38.0 35.0 40.0 28.5 41.0 42-43 35.44903868506079 38.0 35.0 40.0 27.5 41.0 44-45 35.205500897671506 38.0 35.0 41.0 26.5 41.0 46-47 34.732238333109954 38.0 35.0 40.0 23.5 41.0 48-49 34.346710569656054 38.0 34.0 40.0 23.0 41.0 50-51 33.14318295915841 37.0 32.5 39.0 20.0 40.5 52-53 33.192778268701744 37.5 33.0 39.5 20.0 40.5 54-55 33.717145260741205 38.0 33.5 40.0 20.0 41.0 56-57 33.677372847538194 38.0 34.0 40.0 19.5 41.0 58-59 33.52385284699301 38.0 33.5 40.0 18.5 41.0 60-61 33.144838975677686 37.0 33.0 40.0 14.0 41.0 62-63 32.6842990400859 36.0 32.5 39.5 10.5 41.0 64-65 32.19115064752745 36.0 32.0 39.0 8.0 41.0 66-67 31.7592801666466 35.0 31.0 39.0 4.5 40.5 68-69 31.39453552409099 35.0 31.0 38.0 2.0 40.0 70-71 30.87631025586856 35.0 31.0 37.5 2.0 39.5 72-73 30.359407074908436 35.0 30.0 37.0 2.0 39.0 74-75 29.915035448748675 35.0 30.0 36.0 2.0 39.0 76-77 29.396915432604974 34.0 29.0 36.0 2.0 37.5 78-79 28.956900409574455 34.0 29.0 35.0 2.0 37.0 80-81 28.52694756099131 34.0 29.0 35.0 2.0 37.0 82-83 28.142329205785984 34.0 27.5 35.0 2.0 36.0 84-85 27.805901537058954 34.0 27.0 35.0 2.0 36.0 86-87 27.351694142002472 34.0 26.0 35.0 2.0 35.5 88-89 26.78796779437833 33.5 25.0 35.0 2.0 35.0 90-91 26.226213976718846 33.0 24.0 35.0 2.0 35.0 92-93 25.826967096988653 33.0 23.0 35.0 2.0 35.0 94-95 25.36172754250026 33.0 19.5 35.0 2.0 35.0 96-97 24.643846653021757 32.0 8.5 35.0 2.0 35.0 98-99 23.854804757393957 32.0 2.0 35.0 2.0 35.0 100-101 21.463544163090745 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06754801601823957 1101 2 0.06128793844026248 1101 3 0.1850326512479974 1101 4 -0.08045807072727484 1101 5 -0.15941561722572573 1101 6 0.029017097524750568 1101 7 0.09050386366212848 1101 8 0.13732165909421923 1101 9 0.087470170971649 1101 10-11 0.026531928222475187 1101 12-13 0.025841636402304857 1101 14-15 0.2882615256673873 1101 16-17 0.29051082916085136 1101 18-19 0.15138806628849721 1101 20-21 0.2900157116576665 1101 22-23 0.21086522296996435 1101 24-25 0.2651000140680253 1101 26-27 0.09359457704473328 1101 28-29 0.239556391438434 1101 30-31 0.1464729654798731 1101 32-33 0.1649985856989744 1101 34-35 0.06848574853568579 1101 36-37 0.20226055070318694 1101 38-39 0.05581722601552741 1101 40-41 0.2281320298444811 1101 42-43 0.49488215109212774 1101 44-45 0.5776913544233366 1101 46-47 0.4824123854743618 1101 48-49 0.2691273482490004 1101 50-51 0.4602950960335832 1101 52-53 0.15965340748551426 1101 54-55 0.15511546641382523 1101 56-57 0.31637544122376937 1101 58-59 0.3497039931289052 1101 60-61 0.1151420970262933 1101 62-63 -0.00941636721461947 1101 64-65 0.09860648184974963 1101 66-67 -0.06275972787097217 1101 68-69 0.11608899662413563 1101 70-71 -0.018067633702063546 1101 72-73 -0.12274362084301771 1101 74-75 -0.24181578327367603 1101 76-77 -0.2415322444944401 1101 78-79 -0.4111240016846196 1101 80-81 -0.2845775980794478 1101 82-83 -0.2673449148529947 1101 84-85 -0.22056554401415696 1101 86-87 -0.42884445701148266 1101 88-89 -0.35601154010634417 1101 90-91 -0.47542678544821015 1101 92-93 -0.5173157229746401 1101 94-95 -0.4547218182196495 1101 96-97 -0.5386734775299935 1101 98-99 -0.5633791646409243 1101 100-101 -0.02189475656026474 1102 1 0.19538564175841344 1102 2 0.11641504600013164 1102 3 0.10330451160803733 1102 4 0.24351401079278645 1102 5 0.0792612275579927 1102 6 0.30331021778348344 1102 7 0.2802187337008917 1102 8 0.3123531684519065 1102 9 0.41478105537392196 1102 10-11 0.28026013920437975 1102 12-13 0.3504439050760553 1102 14-15 0.5379898317718883 1102 16-17 0.5968299416670959 1102 18-19 0.5838284363153718 1102 20-21 0.5381061256787376 1102 22-23 0.5556275354001059 1102 24-25 0.46793347819491515 1102 26-27 0.6079830576991796 1102 28-29 0.563400651979201 1102 30-31 0.5053704946706219 1102 32-33 0.5320350414313069 1102 34-35 0.4250278046104583 1102 36-37 0.695821940444219 1102 38-39 0.5508583665353726 1102 40-41 0.5264846255021283 1102 42-43 0.4575037296514992 1102 44-45 0.5581585252397261 1102 46-47 0.5495243447618918 1102 48-49 0.5682468697823779 1102 50-51 0.8309741524174967 1102 52-53 0.8519924719548513 1102 54-55 0.7625701048290807 1102 56-57 0.7638933361608764 1102 58-59 0.528205098929007 1102 60-61 0.6152497044553655 1102 62-63 0.5456072945294466 1102 64-65 0.48344215757683884 1102 66-67 0.5331529733727614 1102 68-69 0.6413844711669547 1102 70-71 0.6543206567072026 1102 72-73 0.43508437373499476 1102 74-75 0.4764477538598051 1102 76-77 0.5618784948458888 1102 78-79 0.5720737819590518 1102 80-81 0.5361008637882847 1102 82-83 0.5590409023689524 1102 84-85 0.6937397043747069 1102 86-87 0.47977840570283803 1102 88-89 0.4890979697745159 1102 90-91 0.2139712074651534 1102 92-93 0.37572249252558265 1102 94-95 0.33514168086479756 1102 96-97 0.1301084777148276 1102 98-99 0.2909596144606432 1102 100-101 0.5540505241702753 1103 1 -0.09206570999869967 1103 2 0.13245712889558092 1103 3 0.17920154478700567 1103 4 0.18526199845894098 1103 5 0.1829948719321166 1103 6 0.0838382172291503 1103 7 -0.023134963565567546 1103 8 0.15393601193448347 1103 9 0.24229199761965248 1103 10-11 0.14030448561451436 1103 12-13 0.19873529794598 1103 14-15 0.1930815849563956 1103 16-17 0.2767660782170793 1103 18-19 0.34380868830461253 1103 20-21 0.3234423615462063 1103 22-23 0.3206135039187785 1103 24-25 0.25727532665492703 1103 26-27 0.3091263635854702 1103 28-29 0.304201081585056 1103 30-31 0.5190122126320702 1103 32-33 0.5509544441405936 1103 34-35 0.5378114657299662 1103 36-37 0.49841689834976677 1103 38-39 0.5908209492518139 1103 40-41 0.41537723558862893 1103 42-43 0.5389966099739283 1103 44-45 0.6688311124601043 1103 46-47 0.6616231772040564 1103 48-49 0.8084375362488672 1103 50-51 0.7846696865954144 1103 52-53 0.7125056892638142 1103 54-55 0.6977400416585411 1103 56-57 0.7641364330185141 1103 58-59 0.6306822616826437 1103 60-61 0.5353782397534914 1103 62-63 0.3654840715617098 1103 64-65 0.26677010158950054 1103 66-67 0.2511450279742711 1103 68-69 0.1594152133918918 1103 70-71 0.37028594178302043 1103 72-73 0.3743667471918215 1103 74-75 0.265083986262713 1103 76-77 0.20940999350048273 1103 78-79 0.25705316780746656 1103 80-81 0.3933106199985126 1103 82-83 0.34991582008557387 1103 84-85 0.4532129752689116 1103 86-87 0.3581404756639195 1103 88-89 0.35624120636953904 1103 90-91 0.4619126794426123 1103 92-93 0.43966793720228026 1103 94-95 0.3626122032054546 1103 96-97 0.3170511160952607 1103 98-99 0.353849522187776 1103 100-101 0.6775570086779048 1104 1 0.17237116271137154 1104 2 0.17354085383187723 1104 3 0.06934743083612105 1104 4 0.2545500620436556 1104 5 0.19235429427870088 1104 6 0.13366104757023578 1104 7 0.09084158042115575 1104 8 0.13661176468239944 1104 9 0.24335747006463748 1104 10-11 0.19440817226074358 1104 12-13 0.249675479756867 1104 14-15 0.2813796851799779 1104 16-17 0.31359962799193397 1104 18-19 0.19659386674614154 1104 20-21 0.21379855288943617 1104 22-23 0.1531156571772243 1104 24-25 0.25415868029359245 1104 26-27 0.22228533876833723 1104 28-29 0.19335122506124236 1104 30-31 0.4160501992350092 1104 32-33 0.516027587987189 1104 34-35 0.5764289845514625 1104 36-37 0.5873782691163356 1104 38-39 0.6613674975673689 1104 40-41 0.6714783204738737 1104 42-43 0.590907431603803 1104 44-45 0.7472699666142262 1104 46-47 0.7128696126129981 1104 48-49 0.7858891269824895 1104 50-51 0.843334822551931 1104 52-53 0.7761277823649095 1104 54-55 0.6790864641256249 1104 56-57 0.687798400449644 1104 58-59 0.8151914080104987 1104 60-61 0.6984513077339827 1104 62-63 0.7363242663144547 1104 64-65 0.623359741850301 1104 66-67 0.9195549414422963 1104 68-69 0.6952491157209799 1104 70-71 0.7562307425006232 1104 72-73 0.6217633126888131 1104 74-75 0.6952620707775488 1104 76-77 0.6537962367450518 1104 78-79 0.528438482807978 1104 80-81 0.6084730180813338 1104 82-83 0.6218288507577903 1104 84-85 0.6776438679451537 1104 86-87 0.6069879803386122 1104 88-89 0.7926079564889186 1104 90-91 0.7766443142678305 1104 92-93 0.7603494825501294 1104 94-95 0.6535129569574814 1104 96-97 0.6902950389974514 1104 98-99 0.7311305257624063 1104 100-101 1.103932304890403 1105 1 0.1666332309997891 1105 2 0.20810009409981944 1105 3 0.2608959472075796 1105 4 0.220057762452889 1105 5 0.29948503834663853 1105 6 0.22226484355290665 1105 7 0.2302689698686251 1105 8 0.23789970331118582 1105 9 0.11899396433675236 1105 10-11 0.26965395611374987 1105 12-13 0.2713525445420544 1105 14-15 0.22522984516819378 1105 16-17 0.38001872572086626 1105 18-19 0.3574244220413689 1105 20-21 0.12071921479885361 1105 22-23 0.11297356443314754 1105 24-25 0.08140543255507282 1105 26-27 0.19755298990922654 1105 28-29 0.1710694628255922 1105 30-31 0.2606914864595993 1105 32-33 0.2272025530664621 1105 34-35 0.25831070930032496 1105 36-37 0.32103414494584115 1105 38-39 0.08892987512171402 1105 40-41 0.31439387704097754 1105 42-43 0.201250770337019 1105 44-45 0.2970459384812898 1105 46-47 0.5617744631344692 1105 48-49 0.5251546315590048 1105 50-51 0.6110690814516957 1105 52-53 0.6788445697479304 1105 54-55 0.6046991944573321 1105 56-57 0.3341061758324315 1105 58-59 0.4517866489746254 1105 60-61 0.4215355771211122 1105 62-63 0.45512098532873324 1105 64-65 0.3912027793504649 1105 66-67 0.3532630309999938 1105 68-69 0.3621383601392445 1105 70-71 0.21446143346229363 1105 72-73 0.2593894400813852 1105 74-75 0.05139260956074665 1105 76-77 -0.04323751028166711 1105 78-79 0.22187918898749714 1105 80-81 0.40352242030108343 1105 82-83 0.38811551751371454 1105 84-85 0.3476910388090033 1105 86-87 0.38348086900733946 1105 88-89 0.49504604751931325 1105 90-91 0.46569482770282633 1105 92-93 0.6193199795623414 1105 94-95 0.6356833075927462 1105 96-97 0.5144792552783137 1105 98-99 0.57056661749791 1105 100-101 0.8640169179063442 1106 1 0.019004860438251114 1106 2 0.048178629296042885 1106 3 0.09207864721647852 1106 4 0.24128006377886635 1106 5 0.26215301022525495 1106 6 0.30765462730301607 1106 7 0.2607530848459305 1106 8 0.18065132741014622 1106 9 0.1686090751314424 1106 10-11 0.10764563538757699 1106 12-13 0.3163790195798768 1106 14-15 0.4686616997557209 1106 16-17 0.49393647512016514 1106 18-19 0.41738815791394046 1106 20-21 0.2288981104115635 1106 22-23 0.28783173387338934 1106 24-25 0.2197408423619649 1106 26-27 0.22805490323128907 1106 28-29 0.136924450811712 1106 30-31 0.08838014891399126 1106 32-33 0.039974534022235275 1106 34-35 -0.022031018918958978 1106 36-37 0.04640568539257828 1106 38-39 0.10953034677785212 1106 40-41 0.3182616798353166 1106 42-43 0.24204290791822558 1106 44-45 0.3078189428418838 1106 46-47 0.20986336586651078 1106 48-49 0.09591862550757213 1106 50-51 0.29496157944937806 1106 52-53 0.2587279905897901 1106 54-55 0.1946003024042895 1106 56-57 0.33687548703648673 1106 58-59 0.3801499799348491 1106 60-61 0.3567629509753445 1106 62-63 0.27825772111229696 1106 64-65 0.1553157543437962 1106 66-67 0.27361098863993405 1106 68-69 0.14407025922263372 1106 70-71 0.09281975863805414 1106 72-73 0.11171212407177222 1106 74-75 -0.05560770191028652 1106 76-77 0.05463781231333442 1106 78-79 0.174655730769107 1106 80-81 0.23074332000741293 1106 82-83 0.1598884551305133 1106 84-85 0.29007445965086376 1106 86-87 0.5154850738662873 1106 88-89 0.6022210052482428 1106 90-91 0.5487096448248323 1106 92-93 0.6997907457810868 1106 94-95 0.9188304999038621 1106 96-97 1.0179705118414546 1106 98-99 1.061365429458398 1106 100-101 1.418436469388059 1107 1 0.12347093047040048 1107 2 0.2774893860950698 1107 3 0.20711905037922307 1107 4 0.2863252458103176 1107 5 0.13477443693479074 1107 6 -0.03237708700752506 1107 7 0.078256777608118 1107 8 0.166931629782205 1107 9 0.1992872400458623 1107 10-11 0.27823278457517375 1107 12-13 0.12330294867884106 1107 14-15 0.43159994373868216 1107 16-17 0.4445973709408335 1107 18-19 0.3922067781242049 1107 20-21 0.33989330982071664 1107 22-23 0.4852006408157834 1107 24-25 0.2406092931396202 1107 26-27 0.3438338588312533 1107 28-29 0.31899391818507894 1107 30-31 0.19379438994275233 1107 32-33 0.17128318377459806 1107 34-35 0.257154835629656 1107 36-37 0.1735018709864633 1107 38-39 0.0574156973730382 1107 40-41 0.1777097204991449 1107 42-43 0.050455346010153335 1107 44-45 0.1077402001187977 1107 46-47 0.27161721068361544 1107 48-49 0.1576985368812771 1107 50-51 0.34270247787669206 1107 52-53 0.3463507206271501 1107 54-55 0.0741755261422341 1107 56-57 0.12345392564974844 1107 58-59 0.2511797175315067 1107 60-61 0.20461680448129727 1107 62-63 0.017701851792637058 1107 64-65 0.2564985243498725 1107 66-67 0.12634363265765813 1107 68-69 0.1799622785118835 1107 70-71 0.233239212978134 1107 72-73 0.25144190076827044 1107 74-75 0.2424123258940405 1107 76-77 0.2196586655586188 1107 78-79 0.27332199195600637 1107 80-81 0.03820432878315572 1107 82-83 0.04916966983205029 1107 84-85 0.33942170962914275 1107 86-87 0.4167900308564718 1107 88-89 0.5767387739509999 1107 90-91 0.6998315928654968 1107 92-93 0.8177201923012021 1107 94-95 0.8906503799976981 1107 96-97 0.8676810644524515 1107 98-99 0.8896905445856866 1107 100-101 1.2303260775208251 1108 1 0.1426283898500138 1108 2 0.13878451164898564 1108 3 0.028361000157655525 1108 4 -0.06124934798584292 1108 5 0.22591771610760958 1108 6 0.20707815671477903 1108 7 0.24275308484592983 1108 8 0.1828630921160297 1108 9 0.08290319277850244 1108 10-11 0.2951456353875841 1108 12-13 0.3402790195798744 1108 14-15 0.39586464093218865 1108 16-17 0.4801276515907631 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92-93 -2.332307385613852 2316 94-95 -2.5607857052081364 2316 96-97 -3.1503799898770275 2316 98-99 -3.0946939570228587 2316 100-101 -2.8931272528100216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6652.0 3 1378.0 4 1125.0 5 1301.0 6 1564.0 7 1923.0 8 2371.0 9 2880.0 10 3686.0 11 4657.0 12 6652.0 13 8837.0 14 9277.0 15 11267.0 16 14241.0 17 17822.0 18 15805.0 19 10262.0 20 10355.0 21 10978.0 22 12005.0 23 13074.0 24 14516.0 25 16488.0 26 18950.0 27 22083.0 28 25875.0 29 30253.0 30 36155.0 31 43323.0 32 53569.0 33 67673.0 34 92826.0 35 150719.0 36 174437.0 37 219155.0 38 164477.0 39 22021.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.85688689129478 18.13262067813596 13.864357623335408 18.146134807233853 2 13.940481960058884 23.898304956196135 39.56454765334007 22.59666543040491 3 22.69967460911747 30.445859099450956 29.50253659224934 17.351929699182236 4 11.185224903548843 16.931415964439537 39.58467793617652 32.2986811958351 5 11.415308857861934 41.900127811628124 32.34400757762052 14.340555752889422 6 25.427528584726943 37.51577081842866 19.104027846163298 17.952672750681096 7 25.329145610405146 37.906659724235716 20.355187316603693 16.40900734875545 8 24.251579048843674 31.02435314219524 20.638420776570946 24.085647032390142 9 27.476828137310584 20.210004602902938 21.864471428257275 30.448695831529204 10-11 23.581630911056735 26.887346118097838 26.22743223505757 23.303590735787857 12-13 30.631577749281046 25.22236301967539 25.621258223372205 18.524801007671353 14-15 26.19196608035437 25.502764693835754 29.562161998164456 18.74310722764542 16-17 18.927551748750894 29.85948486643279 30.820766321928105 20.392197062888208 18-19 18.28234454808327 30.75022199285069 33.01967198184592 17.947761477220116 20-21 20.790352652432606 28.0142934907337 30.50416039967149 20.691193457162203 22-23 19.407433832514858 27.6541757432694 29.894648156722255 23.043742267493492 24-25 20.852741388136177 27.89546042198787 31.353221601122307 19.898576588753645 26-27 22.20487851575692 25.284502843223994 33.48584314156023 19.02477549945886 28-29 19.129744582028646 27.0711528273332 34.7749355805912 19.02416701004696 30-31 18.87886519652512 29.711704938946603 32.63737014762165 18.77205971690663 32-33 18.692690564895504 29.487707607423303 31.556973174234336 20.262628653446853 34-35 20.24401280702494 26.890725013326406 31.673792445496623 21.19146973415203 36-37 21.759490822738144 26.586922116798196 33.094583644360384 18.55900341610327 38-39 22.603990600338047 25.583640879409515 33.49366769215803 18.31870082809441 40-41 19.177139844366668 27.632133732533415 34.86479895525257 18.32592746784735 42-43 18.353044173459445 29.865570601083096 31.83743990612969 19.943945319327767 44-45 19.033654321175316 29.70077447500627 30.171615757032217 21.093955446786193 46-47 19.539848972564997 28.75947721857174 29.11504155724715 22.585632251616115 48-49 20.788038365355778 28.2105023888071 29.846195716834085 21.155263529003037 50-51 21.502752553010023 29.089339007195708 30.640301146478997 18.767607293315272 52-53 19.361850602087255 29.71660356045236 31.274862087392926 19.646683750067464 54-55 17.536327119473114 28.68421962776128 32.33986821787036 21.43958503489524 56-57 20.21192232149405 30.48391564600932 28.718757587721182 20.58540444477545 58-59 20.19651306129969 28.331158507746746 29.748233072924084 21.724095358029473 60-61 19.824923307583113 27.41950429242084 31.62860591072767 21.12696648926838 62-63 22.565398111645916 27.119990210898887 30.47986356060313 19.834748116852076 64-65 21.03676767154553 28.337120176811442 31.662484475969162 18.963627675673866 66-67 19.321353597608308 28.444193029156317 31.54994483816788 20.684508535067494 68-69 18.634585759538428 30.66169437191386 30.65078310220704 20.05293676634067 70-71 19.683196246865894 30.125592367512123 28.6563878745483 21.534823511073682 72-73 19.497696689908846 28.502844639282404 28.803350766870352 23.196107903938397 74-75 19.65622746553855 30.11560071813777 29.279319175593656 20.948852640730028 76-77 21.211023623842838 27.430368821103464 30.473225589322844 20.88538196573085 78-79 19.703579975119748 27.62616241470686 32.16502280183266 20.505234808340735 80-81 19.471362759407963 30.826392797169394 30.049085680866693 19.653158762555954 82-83 19.189686126604368 29.78160206624071 29.241402951902746 21.787308855252178 84-85 19.555141890399934 28.31681565326516 29.24452183986066 22.883520616474254 86-87 20.915503127683994 28.45390561462981 29.947707251818468 20.682884005867734 88-89 21.88323317335101 26.61064244786639 30.299066102547805 21.207058276234793 90-91 19.87086326217571 27.341976662630746 31.806488769437514 20.980671305756033 92-93 19.355429770507758 30.11160994301659 30.36037542908625 20.172584857389406 94-95 19.132943625710364 30.186804179523012 29.16876398103977 21.511488213726853 96-97 18.900491533600803 28.581449007143185 28.639730468202007 23.878328991054005 98-99 20.690887442052848 28.55516279946807 28.142376067037354 22.611573691441727 100-101 21.992485346577855 27.803533544193627 29.28385033631989 20.92013077290863 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12969.0 1 8083.5 2 5031.0 3 6087.5 4 4216.5 5 3715.0 6 6354.0 7 11832.5 8 16563.5 9 16104.5 10 11049.5 11 6885.0 12 5901.0 13 5714.5 14 5430.5 15 4892.0 16 4507.0 17 4260.5 18 4015.5 19 3967.0 20 4067.5 21 4318.5 22 4705.5 23 5212.5 24 5590.5 25 6173.5 26 7329.5 27 8371.5 28 9092.5 29 10156.0 30 11743.0 31 13303.0 32 15860.0 33 18837.5 34 21664.5 35 23787.5 36 27170.0 37 31034.0 38 31198.5 39 30134.5 40 29145.5 41 28486.5 42 27728.0 43 28147.0 44 31739.5 45 34500.5 46 38873.5 47 57596.5 48 136993.5 49 136790.0 50 63063.0 51 43291.5 52 28484.5 53 24183.5 54 23213.5 55 21416.5 56 19246.0 57 17367.5 58 15770.0 59 14523.5 60 13504.0 61 12247.0 62 10316.0 63 7740.5 64 5507.5 65 4296.5 66 3299.5 67 2605.0 68 2051.5 69 1493.5 70 1201.0 71 967.0 72 806.0 73 741.0 74 614.5 75 487.0 76 366.0 77 252.0 78 176.5 79 135.5 80 113.0 81 84.5 82 67.0 83 50.5 84 35.0 85 24.0 86 14.5 87 10.0 88 6.0 89 3.5 90 4.5 91 5.0 92 3.5 93 2.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2649474989001536 2 0.16264853604959417 3 0.1917253693098568 4 0.15871104821226695 5 0.23284182884194699 6 0.19233113666944562 7 0.2375365258787602 8 0.24473001327387725 9 0.14432407342203282 10-11 0.20172053074307209 12-13 0.20815680893870314 14-15 0.1687819305654308 16-17 0.28009168288987374 18-19 0.22935866652431133 20-21 0.19358053184859753 22-23 0.2384451769181434 24-25 0.22674629478608463 26-27 0.33616302411181254 28-29 0.3082977255707275 30-31 0.2846349380867898 32-33 0.18672778859324918 34-35 0.35153437086137845 36-37 0.38655529633760627 38-39 0.36701929899086727 40-41 0.3286287925769268 42-43 0.36202171827425966 44-45 0.37171399602768057 46-47 0.3773930650238256 48-49 0.39628543455100146 50-51 0.3886754820961671 52-53 0.3885997611762185 54-55 0.3830342735599963 56-57 0.36190813689433676 58-59 0.3363144659517097 60-61 0.3040952145135802 62-63 0.37027529854865715 64-65 0.4043118520655531 66-67 0.41025594428151824 68-69 0.4153292459180745 70-71 0.41430701349876836 72-73 0.40571268908460223 74-75 0.40075296882796885 76-77 0.38485157563876277 78-79 0.35861427687657266 80-81 0.36940450796924823 82-83 0.379588971702335 84-85 0.3807626459615383 86-87 0.3762572512245966 88-89 0.38443511057904545 90-91 0.4063563169041653 92-93 0.4046525962053218 94-95 0.4138148275191025 96-97 0.4141555716588712 98-99 0.4113160371607987 100-101 0.38435938965909683 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1320639.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.950343521222333 #Duplication Level Percentage of deduplicated Percentage of total 1 71.73655915681786 21.485345897771825 2 12.577387094260889 7.533941281450041 3 5.235131734958192 4.703819815225516 4 2.8446896351750652 3.4079772713901537 5 1.77251351380914 2.6543694317296302 6 1.1260493621328278 2.0235339130638885 7 0.7885146878085879 1.6531400039977617 8 0.5436394457165787 1.3025750520718749 9 0.4240065266932728 1.1429227016733465 >10 2.5411929671781035 14.226405159856506 >50 0.24999071472006631 5.165462612082174 >100 0.1319579917140531 7.253818559449695 >500 0.010384410085975258 2.0688675798671734 >1k 0.012917193033774104 8.279642494033013 >5k 0.0030393395373586123 6.262889167360161 >10k+ 0.002026226358239075 10.835289058977281 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 28773 2.178718029681086 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 24402 1.8477418885857528 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20637 1.5626526249792712 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 17064 1.2921017780029214 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14797 1.1204424524794436 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12841 0.9723323330599809 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12503 0.9467386621173537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11820 0.8950212737924595 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 9923 0.7513786886499642 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9580 0.7254064131075941 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8995 0.6811096749376627 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7766 0.5880486643208326 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7145 0.5410259730327516 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7141 0.5407230893529571 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5803 0.4394084984617295 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5309 0.4020023640071208 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5292 0.4007151083679946 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5228 0.39586896949128414 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5227 0.3957932485713356 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5152 0.39011417957519046 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4472 0.33862395401014206 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4417 0.33445930341296903 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4142 0.31363605042710385 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4023 0.30462526095322034 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3955 0.2994762383967155 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3781 0.286300798325659 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3695 0.2797887992100794 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3346 0.25336219814801775 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3286 0.2488189429511017 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3187 0.24132257187619022 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3182 0.24094396727644724 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3134 0.2373093631189144 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2915 0.22072648165017086 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2705 0.20482508846096475 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2618 0.1982373684254365 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2549 0.19301262494898302 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2443 0.18498620743443137 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2411 0.18256313799607615 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2407 0.18226025431628173 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2085 0.15787811809283234 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2073 0.1569694670534491 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2066 0.15643942061380892 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1922 0.14553560814121044 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1851 0.1401594228248598 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1827 0.13834212074609337 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1811 0.13713058602691575 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1759 0.13319309818958852 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1709 0.1294070521921585 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1699 0.1286498429926725 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1589 0.12032054179832642 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1526 0.11555012384156457 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1485 0.11244556612367196 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1422 0.1076751481669101 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1385 0.1048734741288119 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1372 0.10388910216948008 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1365 0.10335905572983986 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1361 0.10305617205004548 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1343 0.10169319549097067 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.572091994860064E-5 0.0 14-15 0.0 0.0 0.0 2.2716275984580194E-4 0.0 16-17 0.0 0.0 0.0 4.164650597173035E-4 0.0 18-19 0.0 0.0 0.0 5.679068996145048E-4 0.0 20-21 0.0 0.0 0.0 0.0010222324193061086 0.0 22-23 0.0 0.0 0.0 0.0029152554180211245 0.0 24-25 0.0 0.0 0.0 0.005906231755990849 0.0 26-27 0.0 0.0 0.0 0.009730138213395183 0.0 28-29 0.0 0.0 0.0 0.019876741486507667 0.0 30-31 0.0 0.0 0.0 0.04285804069090796 0.0 32-33 0.0 0.0 0.0 0.07996129146572228 0.0 34-35 0.0 0.0 0.0 0.1417495621437804 0.0 36-37 0.0 0.0 0.0 0.22954796882418282 0.0 38-39 0.0 0.0 0.0 0.33726097745106726 0.0 40-41 0.0 0.0 0.0 0.4608375188071835 0.0 42-43 0.0 0.0 0.0 0.5816502465851758 0.0 44-45 0.0 0.0 0.0 0.7142754378751498 0.0 46-47 0.0 0.0 0.0 0.889266483876366 0.0 48-49 0.0 0.0 0.0 1.0945837583169966 0.0 50-51 0.0 0.0 0.0 1.295092754340891 0.0 52-53 0.0 0.0 0.0 1.4901877045884606 0.0 54-55 0.0 0.0 0.0 1.6835789341371865 0.0 56-57 0.0 0.0 0.0 1.9122561123819606 0.0 58-59 0.0 0.0 0.0 2.216313466435566 0.0 60-61 0.0 0.0 0.0 2.6199816906815565 0.0 62-63 0.0 0.0 0.0 2.965496248407021 0.0 64-65 0.0 0.0 0.0 3.2512670002930397 0.0 66-67 0.0 0.0 0.0 3.5812587694290414 0.0 68-69 0.0 0.0 0.0 3.956455927774358 0.0 70-71 0.0 0.0 0.0 4.407146843308428 0.0 72-73 0.0 0.0 0.0 4.871581105813171 0.0 74-75 0.0 0.0 0.0 5.31750160339048 0.0 76-77 0.0 0.0 0.0 5.7374498254254185 0.0 78-79 0.0 0.0 0.0 6.231036642110372 0.0 80-81 0.0 0.0 0.0 6.77334987078225 0.0 82-83 0.0 0.0 0.0 7.318124029352457 0.0 84-85 0.0 0.0 0.0 8.016346632198504 0.0 86-87 0.0 0.0 0.0 8.74054151058692 0.0 88-89 0.0 0.0 0.0 9.343015010157961 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 680 0.0 39.070538 2 GTACGTG 90 5.6695717E-7 36.968716 1 GTGCGAA 75 3.1025993E-4 31.62493 9 GGGATAC 205 1.8189894E-12 30.085676 7 GATACGC 65 0.005882165 29.192242 9 TACGTGG 115 3.9015667E-6 28.878223 2 CGACCGT 50 4.9808834E-5 28.478651 18-19 GCTTATG 315 0.0 27.160688 1 CATGGGT 740 0.0 25.644756 4 CCTTTAG 280 0.0 25.463144 1 AGTACTC 630 0.0 24.097927 5 TTAGGCA 240 2.3283064E-10 23.7214 4 GGATACG 100 0.0016657807 23.720497 8 ACCTGGG 1105 0.0 23.61406 3 CATGGGG 2705 0.0 23.502161 4 ATGGGAG 2585 0.0 23.124922 5 AGTACTT 11750 0.0 22.846983 12-13 GAGTACT 11505 0.0 22.755848 12-13 CATATAG 275 5.2750693E-11 22.427504 3 GAGTACG 235 4.614776E-9 22.24865 1 >>END_MODULE